CN105659976B - The cryogenic treatment process of seeds of Gastrodia elata - Google Patents
The cryogenic treatment process of seeds of Gastrodia elata Download PDFInfo
- Publication number
- CN105659976B CN105659976B CN201610050198.3A CN201610050198A CN105659976B CN 105659976 B CN105659976 B CN 105659976B CN 201610050198 A CN201610050198 A CN 201610050198A CN 105659976 B CN105659976 B CN 105659976B
- Authority
- CN
- China
- Prior art keywords
- seed
- capsule
- rhizoma gastrodiae
- low
- seeds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000305491 Gastrodia elata Species 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000002775 capsule Substances 0.000 claims abstract description 41
- 238000009331 sowing Methods 0.000 claims abstract description 13
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims description 13
- 230000035784 germination Effects 0.000 claims description 12
- 239000002985 plastic film Substances 0.000 claims description 12
- 229920006255 plastic film Polymers 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 12
- 210000001161 mammalian embryo Anatomy 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000004698 Polyethylene Substances 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 8
- 229920000573 polyethylene Polymers 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 7
- 238000009395 breeding Methods 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 235000007466 Corylus avellana Nutrition 0.000 claims description 5
- 240000003211 Corylus maxima Species 0.000 claims description 5
- 239000004744 fabric Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 231100000252 nontoxic Toxicity 0.000 claims description 5
- 230000003000 nontoxic effect Effects 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 230000035800 maturation Effects 0.000 claims description 3
- 241000305492 Gastrodia Species 0.000 abstract description 7
- 230000007226 seed germination Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 3
- 230000015572 biosynthetic process Effects 0.000 abstract description 2
- 230000000284 resting effect Effects 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 5
- 239000000835 fiber Substances 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000005336 cracking Methods 0.000 description 3
- PUQSUZTXKPLAPR-KSSYENDESA-N 4-(beta-D-Glucopyranosyloxy) benzyl alcohol Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-KSSYENDESA-N 0.000 description 2
- 241000837768 Gastrodia elata f. glauca Species 0.000 description 2
- PUQSUZTXKPLAPR-UJPOAAIJSA-N Gastrodin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(CO)C=C1 PUQSUZTXKPLAPR-UJPOAAIJSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000010408 film Substances 0.000 description 2
- 229930193974 gastrodin Natural products 0.000 description 2
- PUQSUZTXKPLAPR-NZEXEKPDSA-N helicidol Natural products O([C@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-NZEXEKPDSA-N 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 206010010904 Convulsion Diseases 0.000 description 1
- 244000086443 Craterellus fallax Species 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 206010063006 Facial spasm Diseases 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000593922 Quercus acutissima Species 0.000 description 1
- 241001480066 Quercus aliena Species 0.000 description 1
- 240000008289 Quercus suber Species 0.000 description 1
- 235000016977 Quercus suber Nutrition 0.000 description 1
- 108010016634 Seed Storage Proteins Proteins 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 210000004700 fetal blood Anatomy 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000002631 hypothermal effect Effects 0.000 description 1
- 231100000862 numbness Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a kind of cryogenic treatment process of seeds of Gastrodia elata, capsule is gathered, clean after be put into freezer and carry out multi-period, multi-temperature low-temperature treatment, then carry out the sowing of seed.The present invention applies low-temperature treatment seeds of Gastrodia elata, has gastrodia seed germination rate high, plants the features such as numb (protocorm) formation rate is high, and seeds of Gastrodia elata is largely sprouted, and rhizoma Gastrodiae seedling is healthy and strong, and storage rate and survival rate are high；The method of the invention, which overcomes seeds of Gastrodia elata, needs the natural low temperature resting state of 4~6 months, effectively shortens the crop cycle of rhizoma Gastrodiae, and yield is high, profitable.
Description
Technical field
The present invention relates to a kind of cryogenic treatment process of seeds of Gastrodia elata.
Background technology
Rhizoma Gastrodiae (Gastrodia elata Blmue.) is the perennial symbiosis herbaceous plant of orchid family Gastrodia, mainly with stem tuber
It is used as medicine, rhizoma Gastrodiae cures mainly the diseases such as convulsion, extremity numbness, dizziness of having a headache, coronary heart disease, facial spasm, before more than 2,000 years just
Quilt《Sheng Nong's herbal classic》Top grade is classified as, its fresh goods rhizoma Gastrodiae is also one of dietotherapy dish.
Rhizoma Gastrodiae traditional cultivation area of China is more in the ground such as Zhaotong County, Yunnan, Hubei, Hunan, Shaanxi, Anhui, its natural range
For broadleaf deciduous woods distributed area, it is bacterium material that gastrodin cultivation, which is also required to broad leaf tree timber, with the fallen leaves species of Fagaceae Quercus acutissima category such as
Cork oak, oriental white oak etc. are preponderant strains material.Since cultivation rhizoma Gastrodiae needs to expend substantial amounts of bacterium material and timber, in recent years, with Yunnan
The forest reserves especially bacterium material broad-leaf forest timber in the rhizoma Gastrodiae tradition producing region such as Zhaotong, Hubei Province, Shan, Anhui, expensive is increasingly deficient, rhizoma Gastrodiae plantation institute
Need raw material more and more nervous, the yield and area of rhizoma Gastrodiae have the trend of increasingly atrophy.To explore rhizoma Gastrodiae in traditional producing region with outskirt
The possibility of domain plantation, will be distributed over the rhizoma Gastrodiae Introduced Cultivars in the NATURAL DISTRIBUTION such as Zhaotong County, Yunnan, Anhui, river, expensive, Shan region to traditional
Broad-leaf forest timber resources expands manual scale planting and the developing industrialization of rhizoma Gastrodiae compared with the area of horn of plenty on the south cultural area
New region has become the inexorable trend of Tianma industry development.Seeds of Gastrodia elata can normally be sprouted in original area after natural low temperature is handled
Hair, but during the area especially South Subtropical Area of China introducing and planting on the south rhizoma Gastrodiae tradition planting area, due to these areas
Domain reaches the hypothermic phase of 4-6 months or so without traditional gastrodin cultivation head of district under natural conditions, it is impossible to meets seeds of Gastrodia elata to low temperature
Demand, produced gastrodia seed germination rate is extremely low after artificial growth, less than 10%, even if sprouting formed kind fiber crops（Protocorm）
For conversion ratio also below 1%, kind Taro Aso's growing way is weak.
China carries out the history that rhizoma Gastrodiae artificial cultivation only has 30 years or so, in the past to seeds of Gastrodia elata research to lay particular emphasis on sprouting more
The research of bacterium（Peng Shumin, Chen Yuhui, Ao Xinyu halimasch sprout and grow to Zhaotong gastrodia elata f. glauca seed with bacterium material various combination
Influence Southwest University journal .2012,32（3）：47-54；Xu Jintang, Guo is along star rhizoma Gastrodiaes and Mycen osmundicola and halimasch nutrition
Relation and its application medical research communications .1991,20 in cultivation（10）：31-32）, and in terms of its seed low-temperature treatment
Research, be only limitted to 2-5 DEG C of constant temperature refrigerator and preserve seed, its purpose is also to extend Seed storage service life etc.（Wang Miaomiao, Zhang Yu,
Future -2011 year Chinese botanical gardens Annual Conference opinion is moved towards in Deng Lian, the big gastrodia elata f. glaucas seed Cord blood research botanical garden of Zhao's generation
Collected works .78-81).
The content of the invention
The technical problem to be solved in the present invention is overcome defect described above, there is provided one kind can improve gastrodia seed germination
The cryogenic treatment process of the seeds of Gastrodia elata of rate and the numb conversion ratio of kind.
In order to solve the problems, such as techniques described above, the cryogenic treatment process of seeds of Gastrodia elata of the present invention, including following step
Suddenly：
（1）The collection of capsule：The rhizoma Gastrodiae capsule of maturation is cut with sharp scissors；
（2）The processing of capsule：Shady place is laid with clean nontoxic food-grade polyethylene plastic film in room, by step
（1）The rhizoma Gastrodiae capsule gathered is pending with being laid in after soft cloth clean surface on film；It is or the capsule after cleaning is artificial
Separate seed to be put into the test tube of cleaning, clogged with tampon, it is pending；
（3）The low-temperature treatment of seed：Clean food-grade polyethylene plastic film is laid with freezer, by step（2）Institute
The clean capsule collected is laid on plastic film, and thickness is no more than 1cm；Or by step（2）It has collected the test tube of capsule seed
The bundled vertical mouth of pipe is put in freezer upwards, carries out low-temperature treatment, i.e.,：Successively regulate and control temperature of ice house for 8 DEG C keep 48 it is small when, 6
DEG C keep 48 it is small when, 4 DEG C keep 48 it is small when, 3 DEG C keep 48 it is small when, 1-3 DEG C store 55-65 days；During low-temperature treatment in freezer
Humidity is 60~70%, and keeps ventilation state；Seed by low-temperature treatment is in spindle, and long 0.6~1mm, wide by 0.14~
0.2 mm, plants skin white translucent, is made of parenchyma cell, no endosperm, embryo is in filbert or dark brown；
（4）The sowing of seed：Through step（3）Rhizoma Gastrodiae capsule after low-temperature treatment takes out seed or with the seed in test tube
Seed, i.e., be uniformly sprinkling upon on the leaf for having cultivated Germination Strain Mycen osmundicola, Bian Sabian is mixed uniformly, and seed dosage is 0.5- by inoculation
0.8 Ke/㎡；The leaf with Germination Strain Mycen osmundicola after inoculation is put into wooden case, sows the gradual shape of 20-30 days protocorms
Into embryo, which dashes forward to break in the seed coat, to be started to germinate, and 30-40 days protocorms of sowing have mamillary bud to protrude bud by piece projection, vegetative propagation corm
By piece continued growth, the production that breeding and seedling material carries out rhizoma Gastrodiae can be used as at this time.
The present invention manually meets its athermobiosis characteristic, to improve it by carrying out Cryogenic Temperature Swing processing to seeds of Gastrodia elata
Seed germination rate and kind fiber crops shorten crop cycle into rate.The method of the invention, the seed low-temperature treatment phase is by original 4-6
The moon shortens to 70 days or so, effectively shortens the crop cycle of rhizoma Gastrodiae, the rhizoma Gastrodiae seedling of breeding is healthy and strong, storage rate and survival rate
Height, gastrodia seed germination rate plant numb survival rate more than 70% up to more than 80%, the method from natural environment temperature limitation,
Self-sufficiency for the higher area kind fiber crops of temperature on the south rhizoma Gastrodiae tradition planting area and propagating materials etc. all has great importance,
Rhizoma Gastrodiae originate in area by this technology handle also can need not overwintering, obtain kind of a fiber crops as early as possible, solve seeds of Gastrodia elata seed collecting after
Without natural low temperature sowing germination rate is low, kind fiber crops are into the problems such as rate is few, the numb propagating materials of kind lacks.
Beneficial effects of the present invention：The present invention applies low-temperature treatment seeds of Gastrodia elata, has gastrodia seed germination rate high, kind is numb
The features such as (protocorm) formation rate is high, seeds of Gastrodia elata is largely sprouted, and rhizoma Gastrodiae seedling is healthy and strong, and storage rate and survival rate are high；The present invention
The method, which overcomes seeds of Gastrodia elata, needs the natural low temperature resting state of 4~6 months, effectively shortens the crop cycle of rhizoma Gastrodiae,
Yield is high, profitable.
Embodiment
The present invention is described in further detail with reference to embodiment.
The cryogenic treatment process of seeds of Gastrodia elata of the present invention, comprises the following steps：
（1）The collection of capsule：The rhizoma Gastrodiae capsule of maturation is cut with sharp scissors；
（2）The processing of capsule：Shady place is laid with clean nontoxic food-grade polyethylene plastic film in room, by step
（1）The rhizoma Gastrodiae capsule gathered is pending with being laid in after soft cloth clean surface on film；It is or the capsule after cleaning is artificial
Separate seed to be put into the test tube of cleaning, clogged with tampon, it is pending；
（3）The low-temperature treatment of seed：Clean food-grade polyethylene plastic film is laid with freezer, by step（2）Institute
The clean capsule collected is laid on plastic film, and thickness is no more than 1cm；Or by step（2）It has collected the test tube of capsule seed
The bundled vertical mouth of pipe is put in freezer upwards, carries out low-temperature treatment, i.e.,：Successively regulate and control temperature of ice house for 8 DEG C keep 48 it is small when, 6
DEG C keep 48 it is small when, 4 DEG C keep 48 it is small when, 3 DEG C keep 48 it is small when, 1-3 DEG C store 55-65 days；During low-temperature treatment in freezer
Humidity is 60~70%, and keeps ventilation state；Seed by low-temperature treatment is in spindle, and long 0.6~1mm, wide by 0.14~
0.2 mm, plants skin white translucent, is made of parenchyma cell, no endosperm, embryo is in filbert or dark brown；
（4）The sowing of seed：Through step（3）Rhizoma Gastrodiae capsule after low-temperature treatment takes out seed or with the seed in test tube
Seed, i.e., be uniformly sprinkling upon on the leaf for having cultivated Germination Strain Mycen osmundicola, Bian Sabian is mixed uniformly, and seed dosage is 0.5- by inoculation
0.8 Ke/㎡；The leaf with Germination Strain Mycen osmundicola after inoculation is put into wooden case, sows the gradual shape of 20-30 days protocorms
Into embryo, which dashes forward to break in the seed coat, to be started to germinate, and 30-40 days protocorms of sowing have mamillary bud to protrude bud by piece projection, vegetative propagation corm
By piece continued growth, the production that breeding and seedling material carries out rhizoma Gastrodiae can be used as at this time.
Embodiment one：
（1）Gather capsule：It is in long oval that rhizoma Gastrodiae, which connects maturescent capsule, and coat color is dimmed to tarnish, and longitudinal furrow is recessed
Fall into obvious, refer to and pinch slightly soft, capsule lower part longitudinal joint line is the appropriate time for gathering rhizoma Gastrodiae capsule when just cracking, and when collection is used
Sharp scissors cuts capsule；Seed after rhizoma Gastrodiae capsule natural cracking, should not collect and handle, and the kind of part natural cracking
Sub- germination percentage reduces speed.
（2）Handle capsule：Shady place is laid with clean nontoxic food-grade polyethylene plastic film in room, by step（1）
The rhizoma Gastrodiae capsule soft cloth wiping gathered is laid on the very thin film of material after removing silt, the dust on surface etc., is waited pending；
（3）Low-temperature treatment seed：Clean nontoxic food-grade polyethylene plastic film is laid with freezer, by step（2）
Collected clean capsule is laid on plastic film, and thickness is no more than 1cm, and it is small for 8 DEG C of holdings 48 to regulate and control temperature of ice house successively
When, 6 DEG C keep 48 it is small when, 4 DEG C keep 48 it is small when, 3 DEG C keep 48 it is small when, 1-3 DEG C store 60 days；During low-temperature treatment in freezer
Humidity is 60~70%, and keeps ventilation state；Seed by low-temperature treatment is in spindle, and long 0.6~1mm, wide by 0.14~
0.2 mm, plants skin white translucent, is made of parenchyma cell, no endosperm, embryo is in filbert or dark brown；
（4）The sowing of seed：Through step（3）Rhizoma Gastrodiae capsule after low-temperature treatment takes out seed, and seed is uniformly sprinkling upon
On the leaf for cultivating Germination Strain Mycen osmundicola, Bian Sabian is mixed uniformly, and seed dressing is indoors or lee side carries out, and seed dosage is 0.5-
0.8 Ke/㎡；The leaf with Germination Strain Mycen osmundicola after inoculation is put into wooden case, 2-3 lis of leaf thickness after inoculation
Rice, then spreads the leaves of 3 cm thicks or so, and temperature is maintained at 22 DEG C -25 DEG C, and humidity sows 20-30 days protocorms 60% or so
Stem gradually forms, and embryo, which dashes forward to break in the seed coat, to be started to germinate, and 30-40 days protocorms of sowing begin with obvious mamillary bud and dashed forward by piece
Rise, vegetative propagation corm protrudes bud by piece continued growth, can be used as the production that breeding and seedling material carries out rhizoma Gastrodiae at this time.Seed is broadcast
Environment temperature is 22 DEG C -25 DEG C during kind, and the leaf relative humidity after inoculation is 60% or so.
Embodiment two：
（1）Gather capsule：With embodiment one；
（2）Handle capsule：By step（1）After the rhizoma Gastrodiae capsule soft cloth clean surface gathered, manual removal capsule, this
When capsule in seed be in cheese color, seed, which is scattered, easily to be shaked out, by the seed separated be put into cleaning test tube in, with tampon plug
Firmly, seed is not only preserved, but also respiratory ventilation can be kept, it is pending；
（3）Low-temperature treatment seed：By step（2）The bundled vertical mouth of pipe of test tube that have collected capsule seed is put in freezer upwards
It is interior, successively regulate and control temperature of ice house for 8 DEG C keep 48 it is small when, 6 DEG C keep 48 it is small when, 4 DEG C keep 48 it is small when, 3 DEG C keep 48 it is small when,
1-3 DEG C is stored 60 days；Humidity is 60~70% in freezer during low-temperature treatment, and keeps ventilation state；By the kind of low-temperature treatment
Son is in spindle, and long 0.6~1mm, wide 0.14~0.2 mm, plant skin white translucent, be made of parenchyma cell, no endosperm,
Embryo is in filbert or dark brown；
（4）The sowing of seed：Through step（3）The seed in test tube after low-temperature treatment, which is uniformly removed, is cultivating Germination Strain purple
On the leaf of the small mushroom of beanstalk, Bian Sabian is mixed uniformly, and seed dressing is indoors or lee side carries out, and seed dosage is 0.5-0.8 grams/㎡；Will
The leaf with Germination Strain Mycen osmundicola after inoculation is put into wooden case, and 20-30 days protocorms of sowing gradually form, and embryo is broken through
Kind skin starts to germinate, and 30-40 days protocorms of sowing begin with obvious mamillary bud by piece projection, and vegetative propagation corm protrudes luxuriant quilt
Piece continued growth, can be used as the production that breeding and seedling material carries out rhizoma Gastrodiae at this time.Environment temperature is 22 DEG C -25 when seed is sowed
DEG C, by means of the leaf relative humidity after kind 60% or so.
It these are only some embodiments of the present invention, as long as having used techniques described above scheme, this hair should all be fallen into
Bright protection domain.
Claims (1)
1. a kind of cryogenic treatment process of seeds of Gastrodia elata, it is characterised in that the treating method comprises following steps：
（1）The collection of capsule：The rhizoma Gastrodiae capsule of maturation is cut with sharp scissors；
（2）The processing of capsule：Shady place is laid with clean nontoxic food-grade polyethylene plastic film in room, by step（1）Institute
The rhizoma Gastrodiae capsule of collection is pending with being laid in after soft cloth clean surface on film；Or by the capsule manual removal after cleaning
Go out seed to be put into the test tube of cleaning, clogged with tampon, it is pending；
（3）The low-temperature treatment of seed：Clean food-grade polyethylene plastic film is laid with freezer, by step（2）It is collected
Clean capsule be laid on plastic film, thickness is no more than 1cm；Or by step（2）The test tube that have collected capsule seed is bundled
The vertical mouth of pipe is put in freezer upwards, carries out low-temperature treatment, i.e.,：Successively regulate and control temperature of ice house for 8 DEG C keep 48 it is small when, 6 DEG C guarantor
Hold 48 it is small when, 4 DEG C keep 48 it is small when, 3 DEG C keep 48 it is small when, 1-3 DEG C store 55-65 days；Humidity in freezer during low-temperature treatment
For 60~70%, and keep ventilation state；Seed by low-temperature treatment is in spindle, long 0.6~1mm, wide by 0.14~0.2
Mm, plants skin white translucent, is made of parenchyma cell, no endosperm, embryo is in filbert or dark brown；
（4）The sowing of seed：Through step（3）Rhizoma Gastrodiae capsule after low-temperature treatment takes out seed or is connect with the seed in test tube
Kind, i.e., seed is uniformly sprinkling upon on the leaf for having cultivated Germination Strain Mycen osmundicola, Bian Sabian is mixed uniformly, and seed dosage is 0.5-
0.8 Ke/㎡；The leaf with Germination Strain Mycen osmundicola after inoculation is put into wooden case, sows the gradual shape of 20-30 days protocorms
Into embryo, which dashes forward to break in the seed coat, to be started to germinate, and 30-40 days protocorms of sowing have mamillary bud to protrude bud by piece projection, vegetative propagation corm
By piece continued growth, the production that breeding and seedling material carries out rhizoma Gastrodiae can be used as at this time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610050198.3A CN105659976B (en) | 2016-01-26 | 2016-01-26 | The cryogenic treatment process of seeds of Gastrodia elata |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610050198.3A CN105659976B (en) | 2016-01-26 | 2016-01-26 | The cryogenic treatment process of seeds of Gastrodia elata |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105659976A CN105659976A (en) | 2016-06-15 |
| CN105659976B true CN105659976B (en) | 2018-04-17 |
Family
ID=56302716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610050198.3A Expired - Fee Related CN105659976B (en) | 2016-01-26 | 2016-01-26 | The cryogenic treatment process of seeds of Gastrodia elata |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105659976B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105471A (en) * | 2016-06-20 | 2016-11-16 | 潜山县富源科技有限公司 | A kind of preprocess method of seeds of Gastrodia elata |
| CN106900506A (en) * | 2017-03-07 | 2017-06-30 | 西南交通大学 | A kind of seeds of Gastrodia elata prolongs long-life storage practice |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1038743A (en) * | 1988-06-24 | 1990-01-17 | 中国医学科学院药用植物资源开发研究所 | Mycen osmundicola and gastrodia seed germination culture technique |
| CN104115634A (en) * | 2013-04-24 | 2014-10-29 | 谭德寅 | Novel gastrodia elata planting and cultivating method |
| CN104127708A (en) * | 2014-07-23 | 2014-11-05 | 陕西理工学院 | Rhizome gastrodiae freshness keeping method |
-
2016
- 2016-01-26 CN CN201610050198.3A patent/CN105659976B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1038743A (en) * | 1988-06-24 | 1990-01-17 | 中国医学科学院药用植物资源开发研究所 | Mycen osmundicola and gastrodia seed germination culture technique |
| CN104115634A (en) * | 2013-04-24 | 2014-10-29 | 谭德寅 | Novel gastrodia elata planting and cultivating method |
| CN104127708A (en) * | 2014-07-23 | 2014-11-05 | 陕西理工学院 | Rhizome gastrodiae freshness keeping method |
Non-Patent Citations (1)
| Title |
|---|
| 地道栽天麻的冬季麻种保存;王敬俊;《食用菌》;19851227;参见第1段和倒数第二段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105659976A (en) | 2016-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fabbri et al. | Olive propagation manual | |
| CN103460943B (en) | Elite breeding method for tea-oil trees | |
| JP2011142903A (en) | Method for raising seedlings of peony | |
| CN104322290A (en) | Litchi tree grafting method | |
| CN107873286A (en) | A kind of mating system of passion fruit female parent seedling | |
| Menzel | Propagation of lychee: a review | |
| CN104904447B (en) | Intelligence development Seed storage and seed seedling Cultivating techniques | |
| CN104322284A (en) | Psidium guajave tree grafting method | |
| CN107087502A (en) | The double sprout tillers grafting seeding cultivating methods of tomato | |
| CN105659976B (en) | The cryogenic treatment process of seeds of Gastrodia elata | |
| CN104904555A (en) | Bonsai manufacturing method of seedling red maple | |
| CN104429486A (en) | Method for culturing immature seeds of processed tomatoes into seedlings | |
| CN114080962B (en) | Bletilla striata seedling growth promoting method based on Piriopsis cumingii | |
| CN103782808B (en) | The engrafting and cultivating method of the anti-blue or green eggplant anvil of Solanum eggplant and tobacco grafting eggplant | |
| CN102017850B (en) | Oil tea double embryo stem anvil grafting nutritive cup breeding method | |
| CN101595832B (en) | Method for water culture of zamioculcas zamiifolia | |
| CN108157044A (en) | A kind of olive green wood cutting method for culturing seedlings | |
| CN208675840U (en) | A kind of shiny-leaved yellowhorn nursery stock cuttage seedbed | |
| CN107926672B (en) | Method for promoting bodhi water culture rooting | |
| CN105850418A (en) | Pruning plantation method for pepper | |
| Al-Jabbari et al. | Comparison of rooting situations for Salakhani and Zivzik pomegranates under different Iba doses. | |
| CN206042897U (en) | A kind of Fructus Litchi fruit tree seedling culture necktie type ring cutting vertical duct-blade | |
| CN110583267A (en) | Bread fruit bud grafting seedling method | |
| CN112544366B (en) | Cultivation method of passion fruit seedlings | |
| CN109105019A (en) | A kind of Moringa engrafting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Sun Yongyu Inventor after: Yang Guangyun Inventor after: Wang Xiang Inventor after: Gao Chengjie Inventor after: Qiu Weidong Inventor after: Li Kun Inventor before: Yang Guangyun Inventor before: Sun Yongyu Inventor before: Wang Xiang Inventor before: Gao Chengjie Inventor before: Qiu Weidong Inventor before: Li Kun |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180327 Address after: 666599 Zhenyuan County Yunnan Pu'er Engelmann Town Green Sea Shengyuan Hall Applicant after: Zhenyuan Sheng Tang Bio Science and Technology Development Co., Ltd. Applicant after: Resources Insect Institute, Chinese Academy of Forestry Sciences Address before: 666599 Simao city of Yunnan province Zhenyuan County town of Pu'er City Enle green sea Shengyuan Hall Applicant before: Zhenyuan Shengyuantang Biological Technology Development Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 666599 Shengyuan Hall of Green Sea Road, Enle Town, Zhenyuan County, Puer City, Yunnan Province Co-patentee after: Resources Insect Institute, Chinese Academy of Forestry Sciences Patentee after: Zhenyuan Shengyuantang Biological Technology Development Co.,Ltd. Address before: 666599 Shengyuan Hall of Green Sea Road, Enle Town, Zhenyuan County, Puer City, Yunnan Province Co-patentee before: Resources Insect Institute, Chinese Academy of Forestry Sciences Patentee before: Zhenyuan Sheng Tang Bio Science and Technology Development Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180417 Termination date: 20200126 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |