A kind of composite pawpaw glycoprotein ferment and production method thereof
Technical field
The invention belongs to field of food, relate to a kind of composite pawpaw glycoprotein ferment and production method thereof.
Background technology
Fructus Chaenomelis, have the title of " hundred benefit fruit kings ". Becoming book in China's science of Chinese drug processing monograph Lei Gong's Treatise on Preparation and Broiling of Materia Medica the earliest in fiveth century of Christian era, in connection with in the discussion of Fructus Chaenomelis effect, this secretary is carried it and " battalion can be adjusted to defend, help essence derived from food ". As the one in fruit, containing abundant nutritional labeling in Fructus Chaenomelis, such as carase, Fructus Chaenomelis ferment, vitamin C, vitamin A. Also being plant amedica while that Fructus Chaenomelis being food, it has the reason of integration of edible and medicinal herbs. Fructus Chaenomelis can be helped digest, absorb, it is also possible to the plant of decomposing protein, saccharide and this three nutritious elements of fat, and especially remove stubborn stool expelling intestinal toxin and decomposition waist and belly fat effect are notable, and this benefits from enzyme abundant in Fructus Chaenomelis.
But; in modern industrialization produces; skin and the seed of Fructus Chaenomelis are all abandoned as leftover bits and pieces; it is unfavorable for environmental conservation on the one hand; containing abundant saccharide in above-mentioned leftover bits and pieces, arbitrarily discharge will cause microbial growth and corruption, contaminated environment; on the other hand, its also lead to enterprise produce economic benefit not high. For above-mentioned leftover bits and pieces, there is not yet effective processing mode at present.
Summary of the invention
The problem that leftover bits and pieces is difficult to utilize is produced for solving Fructus Chaenomelis in prior art, it is desirable to provide the production method of a kind of composite enzyme, it is with the leftover bits and pieces Fructus Chaenomelis skin of Fructus Chaenomelis production, Semen Fructus Chaenomelis for primary raw material, fermented prepared a kind of composite pawpaw glycoprotein ferment with multiple efficacies.
For solving above technical problem, the present invention adopts the following technical scheme that.
A kind of composite pawpaw glycoprotein ferment, it is characterized in that, it is made up of the primary raw material of following weight portion: Fructus Chaenomelis skin 50-60 weight portion, Semen Fructus Chaenomelis 5-10 weight portion, Pericarpium Citri Reticulatae 5-10 weight portion, Fructus Ligustri Lucidi 2-5 weight portion, Semen Ziziphi Spinosae 3-5 weight portion, Calyx Hibisci Sabdariffae 5-7 weight portion, crystal sugar 35-40 weight portion, Sal 3-5 weight portion.
The production method of described composite pawpaw glycoprotein ferment comprises the steps:
(1) Fructus Chaenomelis skin, Semen Fructus Chaenomelis, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Semen Ziziphi Spinosae, Calyx Hibisci Sabdariffae, crystal sugar and Sal are weighed by weight;
(2) adopt beater that the Semen Fructus Chaenomelis weighed is smashed pulping, then after filter and remove residue, obtain filtrate A, standby; The crystal sugar weighed is clayed into power standby;
(3) Fructus Ligustri Lucidi, Semen Ziziphi Spinosae mixing will be weighed, add the water of mixture quality 5-8 times, and heat in hermetic container to seething with excitement and keeping boiling 20-25 minute, cooled and filtered, obtain liquor B;
(4) the Fructus Chaenomelis skin weighed is cleaned, and backward clean Fructus Chaenomelis skin adds filtrate A that step (2) prepares, the liquor B that step (3) prepares, the Pericarpium Citri Reticulatae weighed, Calyx Hibisci Sabdariffae, Sal and the crystal sugar powder after pulverizing, stir, and it is placed on environment bottom fermentation 8-10 days of 25-27 degree Celsius, every 2 days of period stirred stirring once, thus preparing fermenting mixture;
(5) adopting blender that fermenting mixture is carried out high-speed stirred, thus preparing serosity, after homogenizer homogenizing, namely preparing described composite pawpaw glycoprotein ferment.
Further, use in order to convenient, the composite pawpaw glycoprotein ferment that above-mentioned steps (5) prepares can also be carried out vacuum lyophilization, obtain composite pawpaw glycoprotein ferment powder after pulverizing, yet further this composite pawpaw glycoprotein ferment powder is made capsule or is pressed into tablet or makes the form of teabag.
Preferably, described composite pawpaw glycoprotein ferment is made up of the primary raw material of following weight portion: Fructus Chaenomelis skin 55 weight portion, Semen Fructus Chaenomelis 8 weight portion, Pericarpium Citri Reticulatae 8 weight portion, Fructus Ligustri Lucidi 3 weight portion, Semen Ziziphi Spinosae 4 weight portion, Calyx Hibisci Sabdariffae 6 weight portion, crystal sugar 35 weight portion, Sal 4 weight portion.
Preferably, described composite pawpaw glycoprotein ferment is made up of the primary raw material of following weight portion: Fructus Chaenomelis skin 58 weight portion, Semen Fructus Chaenomelis 5 weight portion, Pericarpium Citri Reticulatae 7 weight portion, Fructus Ligustri Lucidi 4 weight portion, Semen Ziziphi Spinosae 5 weight portion, Calyx Hibisci Sabdariffae 7 weight portion, crystal sugar 40 weight portion, Sal 5 weight portion.
In the preparation method of the present invention, the fermenting mixture that first time prepares can also be retained as microbial inoculum, subsequent production is added in mixed material to be fermented according to the amount (weight/mass percentage composition) of 0.5% as strain and ferments, thus realizing the repeatability of method and the stability of fermentative microflora.
The invention have the advantages that and beneficial effect:
First, the present invention with in the productions such as Fructus Chaenomelis canned food, papaya tablet leftover bits and pieces--Fructus Chaenomelis skin and Semen Fructus Chaenomelis are for raw material production composite enzyme, which solve the difficult problem that Fructus Chaenomelis skin and Semen Fructus Chaenomelis etc. in prior art are difficult to utilize, achieve the comprehensive utilization of Fructus Chaenomelis skin and Semen Fructus Chaenomelis, improve the economic benefit of enterprise.
Second, in the ferment preparation process of the present invention, adopting natural fermented mode, with the addition of Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae is as the product of a kind of long-term fermentation, it has certain stable advantage fermentative microflora, in conjunction with the natural flora in Fructus Chaenomelis epidermis face, when pressure condition and the fermentation temperature control of certain brine concentration so that the dominant bacteria such as yeast, lactic acid bacteria can mushroom out, thus forming effective fermentation, prepare composite pawpaw glycoprotein fermentation ferment.
3rd, the preparation process of the composite pawpaw glycoprotein ferment of the present invention have employed the Chinese medicines such as Fructus Ligustri Lucidi, Calyx Hibisci Sabdariffae and Semen Ziziphi Spinosae, wherein Fructus Ligustri Lucidi returns liver, kidney channel, has nourishing the liver and kidney, effect of improving eyesight black hair; Calyx Hibisci Sabdariffae has clearing away summer-heat, skin care, speckle removing, relieves the effect of alcohol, blood pressure lowering, Vitamin C are sick and the effect such as diuresis; Semen Ziziphi Spinosae sweet in the mouth, acid, property is put down, and can nourish conscience, calm the nerves, arresting sweating. Containing significant quantities of fat oil and protein, and containing sterol, triterpenes, Semen Ziziphi Spinosae saponin, vitamin C, there are calmness, hypnosis, analgesia, anticonvulsant action, the present invention obtains the functional component in Semen Ziziphi Spinosae and Fructus Ligustri Lucidi by the method decocted, in combination with Calyx Hibisci Sabdariffae, three with the use of, effect complements each other, mutually collaborative so that obtained product has good YIN nourishing and nourishing the liver and kidney effect, is especially suitable for female consumer.
4th, the composite pawpaw glycoprotein ferment of the present invention does not carry out high-temperature process for Fructus Chaenomelis skin and Semen Fructus Chaenomelis, Calyx Hibisci Sabdariffae in producing, remain abundant enzyme therein and vitamin to greatest extent so that in final products, the activity of the enzyme such as papain is significantly high, has the effect of good facilitating digestion;Simultaneously wherein abundant microorganism and tunning thereof, have good defecating feces excretion, but it be not result in serious diarrhoea, it is possible to gentle relieving constipation. It addition, in the product preparation process of the present invention, by the proportioning of the test of accurate proportioning and crystal sugar, in conjunction with the interpolation of Pericarpium Citri Reticulatae so that the product taste of the present invention is sour-sweet, and local flavor is outstanding, receive welcome and the favorable comment of eater.
In sum, the present invention is by the formula created and technique, achieve the comprehensive utilization of Fructus Chaenomelis skin and Semen Fructus Chaenomelis, its discharge that can reduce garbage in Fructus Chaenomelis production process and pollution to environment, also achieving the recycling of garbage, improve economic benefit, its products taste prepared is sour-sweet simultaneously, unique flavor, also has YIN nourishing and the effect such as nourishing the liver and kidney, relieving constipation.
Detailed description of the invention:
Embodiment 1: a kind of composite pawpaw glycoprotein ferment, is made up of the primary raw material of following weight portion: Fructus Chaenomelis skin 55 weight portion, Semen Fructus Chaenomelis 8 weight portion, Pericarpium Citri Reticulatae 8 weight portion, Fructus Ligustri Lucidi 3 weight portion, Semen Ziziphi Spinosae 4 weight portion, Calyx Hibisci Sabdariffae 6 weight portion, crystal sugar 35 weight portion, Sal 4 weight portion.
The production method of described composite pawpaw glycoprotein ferment comprises the steps:
(1) Fructus Chaenomelis skin, Semen Fructus Chaenomelis, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Semen Ziziphi Spinosae, Calyx Hibisci Sabdariffae, crystal sugar and Sal are weighed by weight;
(2) adopt beater that the Semen Fructus Chaenomelis weighed is smashed pulping, then after filter and remove residue, obtain filtrate A, standby; The crystal sugar weighed is clayed into power standby;
(3) Fructus Ligustri Lucidi, Semen Ziziphi Spinosae mixing will be weighed, add the water of mixture quality 5-8 times, and heat in hermetic container to seething with excitement and keeping boiling 20 minutes, cooled and filtered, obtain liquor B;
(4) the Fructus Chaenomelis skin weighed is cleaned, and backward clean Fructus Chaenomelis skin adds filtrate A that step (2) prepares, the liquor B that step (3) prepares, the Pericarpium Citri Reticulatae weighed, Calyx Hibisci Sabdariffae, Sal and the crystal sugar powder after pulverizing, stir, and it is placed on the environment bottom fermentation 9 days of 25 degrees Celsius, every 2 days of period stirred stirring once, thus preparing fermenting mixture;
(5) adopting blender that fermenting mixture is carried out high-speed stirred, thus preparing serosity, after homogenizer homogenizing, namely preparing described composite pawpaw glycoprotein ferment.
Embodiment 2: a kind of composite pawpaw glycoprotein ferment, is made up of the primary raw material of following weight portion: Fructus Chaenomelis skin 58 weight portion, Semen Fructus Chaenomelis 5 weight portion, Pericarpium Citri Reticulatae 7 weight portion, Fructus Ligustri Lucidi 4 weight portion, Semen Ziziphi Spinosae 5 weight portion, Calyx Hibisci Sabdariffae 7 weight portion, crystal sugar 40 weight portion, Sal 5 weight portion.
The production method of described composite pawpaw glycoprotein ferment comprises the steps:
(1) Fructus Chaenomelis skin, Semen Fructus Chaenomelis, Pericarpium Citri Reticulatae, Fructus Ligustri Lucidi, Semen Ziziphi Spinosae, Calyx Hibisci Sabdariffae, crystal sugar and Sal are weighed by weight;
(2) adopt beater that the Semen Fructus Chaenomelis weighed is smashed pulping, then after filter and remove residue, obtain filtrate A, standby; The crystal sugar weighed is clayed into power standby;
(3) Fructus Ligustri Lucidi, Semen Ziziphi Spinosae mixing will be weighed, add the water of mixture quality 5-8 times, and heat in hermetic container to seething with excitement and keeping boiling 20-25 minute, cooled and filtered, obtain liquor B;
(4) the Fructus Chaenomelis skin weighed is cleaned, and backward clean Fructus Chaenomelis skin adds filtrate A that step (2) prepares, the liquor B that step (3) prepares, the Pericarpium Citri Reticulatae weighed, Calyx Hibisci Sabdariffae, Sal and the crystal sugar powder after pulverizing, stir, and it is placed on environment bottom fermentation 8-10 days of 25-27 degree Celsius, every 2 days of period stirred stirring once, thus preparing fermenting mixture;
(5) adopting blender that fermenting mixture is carried out high-speed stirred, thus preparing serosity, after homogenizer homogenizing, namely preparing described composite pawpaw glycoprotein ferment.
Embodiment 3: safety testing
Acute toxicity test in mice: adopt the feedstuff of composite pawpaw glycoprotein ferment and the mice of embodiment of the present invention 1-2 to carry out mix and feed that (ratio of mix is 1g composite pawpaw glycoprotein ferment: 5g feedstuff, ensure that every day, composite pawpaw glycoprotein ferment intake was about 6g composite pawpaw glycoprotein ferment/kg body weight, namely be equivalent to adult and daily intake 300g composite pawpaw glycoprotein ferment), Continuous Observation 14 days, carries out acute toxicity test. Have no dead and undue toxicity's reaction, off-test, Dissection test mice during test, observe kidney, spleen, stomach, liver, lung, the heart and intestinal, be showed no abnormal change. By standard of acute toxicity, the product of embodiment 1,2 belongs to nontoxic level.
Murine chronic toxicity test: adopt the feedstuff of composite pawpaw glycoprotein ferment and the mice of embodiment of the present invention 1-2 to carry out mix and feed that (ratio of mix is 1g composite pawpaw glycoprotein ferment: 10g feedstuff, ensure that every day, composite pawpaw glycoprotein ferment intake was about 3g composite pawpaw glycoprotein ferment/kg body weight, namely be equivalent to adult and daily intake 150g composite pawpaw glycoprotein ferment), feed 98 days continuously, result shows: test mice behavioral activity is normal, fur is smooth, Normal-weight increases, drinking-water of ingesting is normal, hematology and blood parameters all fluctuate within normal range, bone marrow and routine urinalysis index all fluctuate within normal range, tissue internal organs no abnormality seen, generally speaking toxicity reaction is had no.
By testing above it can be seen that the product of the present invention is safe and nontoxic.
Embodiment 4: flavor evaluation is tested
The reference product that product following and of the present invention is closer to is set:
Comparison 1: Fructus Chaenomelis composite enzyme, the products material of its relative embodiment 1 is without Pericarpium Citri Reticulatae, and all the other raw materials and preparation method be example 1 simultaneously.
Comparison 2: Fructus Chaenomelis composite enzyme, the products material of its relative embodiment 1 is without Sal, and all the other raw materials and preparation method be example 1 simultaneously.
This test has invited 50 rucks as judging panel altogether, adopt ten point system evaluation method, respectively the product of embodiment 1-2 and above 2 comparative experimental example being marked, provide corresponding mark, test process adopts Blind Test (namely judging panel is not aware that to use which product). Concrete evaluation result is shown in table 1 below:
Table 1 Analyses Methods for Sensory Evaluation Results
Sequence number |
Group |
Sensory evaluation average mark |
The overall evaluation |
1 |
Embodiment 1 |
8.97 |
Taste is sour-sweet properly, light flowers and fruits fragrance |
2 |
Embodiment 2 |
8.85 |
Taste is sour-sweet properly, light flowers and fruits fragrance |
3 |
Contrast 1 |
7.05 |
Taste is sweeter, British plain spirits |
4 |
Contrast 2 |
6.43 |
Mouthfeel is poor, slight breakdown of fruit taste |
The result tested from above sensory evaluation, the product of the present invention compares contrast 1, the taste and flavor tool of contrast 2 is greatly improved, it has reacted the meaning that Pericarpium Citri Reticulatae and Sal are creative in the preparation of product of the present invention, and there is also therebetween certain synergism, Pericarpium Citri Reticulatae provides just the dominant microflora of high-quality and the pressure that certain density Sal is survived as strain, most suitable dominant microflora is made to obtain maximized growth, thus obtaining good mouthfeel and flowers and fruits fragrance, and the mediation of also Sal and crystal sugar just, the sour-sweet taste of fermented product making the present invention is suitable, receive the welcome of consumer.
Embodiment 5: edible test
On the basis of safety testing, the product of the present invention has carried out foretasting test in certain scope, foretastes test and mainly have chosen 10 female constipation patients, advises the product 5g of its edible present invention every day, disable after adhering to 10 days, the defecation situation in observing 15 days thereafter.According to reflection, wherein it still is able to normal amount every day ground defecation in after disabling 15 days of 9 persons of foretasting, no longer there is the situation of constipation, 1 person of foretasting symptom starting to continue constipation occur for the 12nd day after disabling, after the product of the edible present invention again, constipation symptom disappears. Through to 10 persons's of foretasting it has been observed that before user takes relatively, its QI and blood is more unobstructed, of pink and healthy-looking, and there are the reaction sleep of part user and halitosis to be obtained for good improvement, express willingness and continue to take the product of the present invention.
It addition, test through inventor, every day takes 50g, adheres to taking 10 days, and diarrhoea phenomenon does not occur in period, also without any untoward reaction.
The specific embodiments of the invention that are only disclosed above, but the present invention is not limited to this, the changes that any person skilled in the art can think of, all should drop within protection scope of the present invention.