CN105639633B - A kind of composite pawpaw glycoprotein ferment and its production method - Google Patents

A kind of composite pawpaw glycoprotein ferment and its production method Download PDF

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CN105639633B
CN105639633B CN201610048532.1A CN201610048532A CN105639633B CN 105639633 B CN105639633 B CN 105639633B CN 201610048532 A CN201610048532 A CN 201610048532A CN 105639633 B CN105639633 B CN 105639633B
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pawpaw
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CN105639633A (en
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黄丽
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Shandong Guohetang Pharmaceutical Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

A kind of composite pawpaw glycoprotein ferment, it is characterized in that, it is made of the primary raw material of following parts by weight: pawpaw skin 50-60 parts by weight, pawpaw seeds 5-10 parts by weight, dried orange peel 5-10 parts by weight, fruit of glossy privet 2-5 parts by weight, semen ziziphi spinosae 3-5 parts by weight, roselle 5-7 parts by weight, rock sugar 35-40 parts by weight, salt 3-5 parts by weight;The formula and technique that the present invention passes through original creation, realize the comprehensive utilization of pawpaw skin and pawpaw seeds, its discharge that can reduce the waste in pawpaw production process and the pollution to environment, also achieve the recycling of waste, improve economic benefit, its product sweet mouthfeel obtained simultaneously, unique flavor also have enriching yin and nourishing liver and kidney, defaecation and other effects.

Description

A kind of composite pawpaw glycoprotein ferment and its production method
Technical field
The invention belongs to field of food, it is related to a kind of composite pawpaw glycoprotein ferment and its production method.
Background technique
Pawpaw is known as the title of " hundred beneficial fruit kings ".At the book science of Chinese drug processing monograph " thunder earliest in the China in fiveth century of Christian era Public big gun toast opinion " in, just about the discussion of pawpaw effect, which, which carries it, " to adjust battalion to defend, to help essence derived from food ".As one in fruit Kind, nutritional ingredient rich in pawpaw, such as papain, Fructus Chaenomelis ferment, vitamin C, vitamin A.Pawpaw is the same of food When be also botanical medicine, it have integration of drinking and medicinal herbs reason.Pawpaw can be helped digest, absorb, can with decomposing protein, carbohydrate and The plant of this fatty three nutritious elements, especially clear stool expelling intestinal toxin and decomposition waist and belly fat significant effect, this is benefited from Enzyme abundant in pawpaw.
However, the skin and seed of pawpaw are all abandoned as leftover bits and pieces, and one side is unfavorable in modern industrial process Environmental protection, carbohydrate abundant is contained in above-mentioned leftover bits and pieces, and any discharge will lead to microbial growth and corruption, pollute environment, separately On the one hand, also leading to enterprise's production, the economic benefit is not high.For above-mentioned leftover bits and pieces, there is not yet effective processing side at present Formula.
Summary of the invention
It is difficult to efficiently use to solve the problems, such as that pawpaw produces leftover bits and pieces in the prior art, the present invention is intended to provide a kind of multiple The production method for closing ferment, using leftover bits and pieces-pawpaw skin of pawpaw production, pawpaw seeds as primary raw material, fermented obtained one kind Composite pawpaw glycoprotein ferment with multiple efficacies.
In order to solve the above technical problems, the present invention adopts the following technical scheme that.
A kind of composite pawpaw glycoprotein ferment, which is characterized in that be made of the primary raw material of following parts by weight: pawpaw skin 50-60 weight Measure part, pawpaw seeds 5-10 parts by weight, dried orange peel 5-10 parts by weight, fruit of glossy privet 2-5 parts by weight, semen ziziphi spinosae 3-5 parts by weight, roselle 5-7 parts by weight, rock sugar 35-40 parts by weight, salt 3-5 parts by weight.
The production method of the composite pawpaw glycoprotein ferment includes the following steps:
(1) pawpaw skin, pawpaw seeds, dried orange peel, the fruit of glossy privet, semen ziziphi spinosae, roselle, rock sugar and salt are weighed by weight;
(2) weighed pawpaw seeds are smashed using beater and is slurried, filtrate A is then obtained after filter and remove residue, it is spare;It will claim The rock sugar taken is clayed into power spare;
(3) fruit of glossy privet, semen ziziphi spinosae mixing will be weighed, 5-8 times of mixture quality of water is added, is heated in closed container It boils and keeps boiling 20-25 minutes, cooled and filtered obtains liquor B;
(4) filtrate A, step made from step (2) are added in the pawpaw skin cleaning weighed pawpaw skin, and cleaning backward (3) liquor B made from, weighed dried orange peel, roselle, salt and smashed rock sugar powder, stir evenly, and are placed on It ferments 8-10 days in the environment of 25-27 degrees Celsius, stirring stirring is primary within during which every 2 days, so that fermenting mixture be made;
(5) high-speed stirred is carried out to fermenting mixture using blender, so that slurries be made, is made after homogenizer homogeneous Obtain the composite pawpaw glycoprotein ferment.
Further, it uses for convenience, it is cold composite pawpaw glycoprotein ferment made from above-mentioned steps (5) can also to be subjected to vacuum Jelly obtains composite pawpaw glycoprotein ferment powder after dry, pulverize, and capsule yet further is made in the composite pawpaw glycoprotein ferment powder or is pressed into Tablet or the form that tea bag is made.
Preferably, the composite pawpaw glycoprotein ferment is made of the primary raw material of following parts by weight: 55 parts by weight of pawpaw skin, pawpaw 8 parts by weight of seed, 8 parts by weight of dried orange peel, 3 parts by weight of the fruit of glossy privet, 4 parts by weight of semen ziziphi spinosae, 6 parts by weight of roselle, 35 weight of rock sugar Part, 4 parts by weight of salt.
Preferably, the composite pawpaw glycoprotein ferment is made of the primary raw material of following parts by weight: 58 parts by weight of pawpaw skin, pawpaw 5 parts by weight of seed, 7 parts by weight of dried orange peel, 4 parts by weight of the fruit of glossy privet, 5 parts by weight of semen ziziphi spinosae, 7 parts by weight of roselle, 40 weight of rock sugar Part, 5 parts by weight of salt.
In preparation method of the invention, the fermenting mixture being prepared for the first time can also be retained as microbial inoculum, rear It adds in mixed material to be fermented and ferments according to 0.5% amount (mass percentage) as strain in continuous production, thus The reproducibility of implementation method and the stability of fermentative microflora.
The invention has the advantages that and the utility model has the advantages that
First, the present invention is with the leftover bits and pieces in the productions such as pawpaw can, papaya tablet -- and pawpaw skin and pawpaw seeds are original Material production composite enzyme, which solve pawpaw skins and pawpaw seeds etc. in the prior art to be difficult to the problem efficiently used, realizes wood The comprehensive utilization of melon skin and pawpaw seeds improves the economic benefit of enterprise.
Second, in ferment preparation process of the invention, by the way of natural fermented, it is added to dried orange peel, dried orange peel is as one The product that kind is fermented for a long time has certain stable advantage fermentative microflora, in conjunction with the natural flora in pawpaw epidermis face, one Under the pressure condition and fermentation temperature control condition of determining brine concentration, the dominant bacterias such as saccharomycete, lactic acid bacteria are given birth to rapidly Composite pawpaw glycoprotein fermentation ferment is made to form effective fermentation in length.
Third uses in the fruit of glossy privet, roselle and semen ziziphi spinosae etc. in the preparation process of composite pawpaw glycoprotein ferment of the invention Medicine, wherein the fruit of glossy privet returns liver and kidney channel, has the effect of nourishing liver and kidney, improving eyesight black hair;Roselle has clearing away summerheat, beauty treatment, disappears Spot relieves the effect of alcohol, blood pressure lowering, Vitamin C disease and diuresis and other effects;Semen ziziphi spinosae is sweet in flavor, sour, mild-natured, can nourish conscience, calms the nerves, arrest sweating. Containing significant quantities of fat oil and protein, and contain sterol, triterpenes, jujuboside, vitamin C, have calmness, hypnosis, analgesia, Anticonvulsant action, the present invention obtain effect ingredient in semen ziziphi spinosae and the fruit of glossy privet by the method decocted, in combination with roselle, Three's is used cooperatively, and complements each other in effect, mutually cooperates with, so that obtained product has good enriching yin and nourishing Liver kidney function effect, is especially suitable for female consumer.
4th, in composite pawpaw glycoprotein ferment production of the invention for pawpaw skin and pawpaw seeds, roselle without high temperature at Reason, remain abundant enzyme and vitamin therein to greatest extent so that in final products the enzymes such as papain activity It is very high, have the function of good promotion digestion;Wherein microorganism abundant and its tunning simultaneously have good defaecation Effect, but it not will lead to serious diarrhea, defaecation that can be mild.In addition, passing through in product preparation process of the invention The proportion of the test and rock sugar that accurately match, in conjunction with the addition of dried orange peel, so that product taste of the invention is sour-sweet, flavor is outstanding, Receive the welcome and favorable comment of eater.
In conclusion the present invention realizes the comprehensive utilization of pawpaw skin and pawpaw seeds by the formula and technique of original creation, The discharge of the waste in pawpaw production process and the pollution to environment can be reduced, the recycling of waste is also achieved, mentions High economic benefit, while its product sweet mouthfeel obtained, unique flavor also have the function such as enriching yin and nourishing liver and kidney, defaecation Effect.
Specific embodiment:
Embodiment 1: a kind of composite pawpaw glycoprotein ferment is made of the primary raw material of following parts by weight: 55 parts by weight of pawpaw skin, wood 8 parts by weight of melon seeds, 8 parts by weight of dried orange peel, 3 parts by weight of the fruit of glossy privet, 4 parts by weight of semen ziziphi spinosae, 6 parts by weight of roselle, 35 weight of rock sugar Measure part, 4 parts by weight of salt.
The production method of the composite pawpaw glycoprotein ferment includes the following steps:
(1) pawpaw skin, pawpaw seeds, dried orange peel, the fruit of glossy privet, semen ziziphi spinosae, roselle, rock sugar and salt are weighed by weight;
(2) weighed pawpaw seeds are smashed using beater and is slurried, filtrate A is then obtained after filter and remove residue, it is spare;It will claim The rock sugar taken is clayed into power spare;
(3) fruit of glossy privet, semen ziziphi spinosae mixing will be weighed, 5-8 times of mixture quality of water is added, is heated in closed container It boils and keeps boiling 20 minutes, cooled and filtered obtains liquor B;
(4) filtrate A, step made from step (2) are added in the pawpaw skin cleaning weighed pawpaw skin, and cleaning backward (3) liquor B made from, weighed dried orange peel, roselle, salt and smashed rock sugar powder, stir evenly, and are placed on 25 It ferments 9 days in the environment of degree Celsius, stirring stirring is primary within during which every 2 days, so that fermenting mixture be made;
(5) high-speed stirred is carried out to fermenting mixture using blender, so that slurries be made, is made after homogenizer homogeneous Obtain the composite pawpaw glycoprotein ferment.
Embodiment 2: a kind of composite pawpaw glycoprotein ferment is made of the primary raw material of following parts by weight: 58 parts by weight of pawpaw skin, wood 5 parts by weight of melon seeds, 7 parts by weight of dried orange peel, 4 parts by weight of the fruit of glossy privet, 5 parts by weight of semen ziziphi spinosae, 7 parts by weight of roselle, 40 weight of rock sugar Part, 5 parts by weight of salt.
The production method of the composite pawpaw glycoprotein ferment includes the following steps:
(1) pawpaw skin, pawpaw seeds, dried orange peel, the fruit of glossy privet, semen ziziphi spinosae, roselle, rock sugar and salt are weighed by weight;
(2) weighed pawpaw seeds are smashed using beater and is slurried, filtrate A is then obtained after filter and remove residue, it is spare;It will claim The rock sugar taken is clayed into power spare;
(3) fruit of glossy privet, semen ziziphi spinosae mixing will be weighed, 5-8 times of mixture quality of water is added, is heated in closed container It boils and keeps boiling 20-25 minutes, cooled and filtered obtains liquor B;
(4) filtrate A, step made from step (2) are added in the pawpaw skin cleaning weighed pawpaw skin, and cleaning backward (3) liquor B made from, weighed dried orange peel, roselle, salt and smashed rock sugar powder, stir evenly, and are placed on It ferments 8-10 days in the environment of 25-27 degrees Celsius, stirring stirring is primary within during which every 2 days, so that fermenting mixture be made;
(5) high-speed stirred is carried out to fermenting mixture using blender, so that slurries be made, is made after homogenizer homogeneous Obtain the composite pawpaw glycoprotein ferment.
Embodiment 3: safety testing
Acute toxicity test in mice: it is mixed using the composite pawpaw glycoprotein ferment of 1-2 of the embodiment of the present invention and the feed of mouse Closing feeding, (ratio of mix is 1g composite pawpaw glycoprotein ferment: 5g feed guarantees that daily composite pawpaw glycoprotein ferment intake is that about 6g is compound Fructus Chaenomelis ferment/kg weight, that is, adult daily intakes 300g composite pawpaw glycoprotein ferment), it is observed continuously 14 days, carries out acute poison Property test.Have no dead and undue toxicity reaction during test, off-test, Dissection test mouse, observation kidney, spleen, stomach, liver, Lung, the heart and intestines, are showed no abnormal change.According to acute toxicity criteria, the product of embodiment 1,2 belongs to nontoxic grade.
Murine chronic toxicity test: it is mixed using the composite pawpaw glycoprotein ferment of 1-2 of the embodiment of the present invention and the feed of mouse Closing feeding, (ratio of mix is 1g composite pawpaw glycoprotein ferment: 10g feed, guarantees that daily composite pawpaw glycoprotein ferment intake is about 3g multiple Fructus Chaenomelis ferment/kg weight is closed, that is, adult daily intakes 150g composite pawpaw glycoprotein ferment), as a result continuous feeding 98 days is shown Show: test mice behavioral activity is normal, and fur is smooth, and Normal-weight increases, and ingesting, it is normal to drink water, and hematology and blood biochemical refer to Mark fluctuates in the normal range, and marrow and routine urinalysis index fluctuate in the normal range, organizes internal organs no abnormality seen, whole For have no toxicity react.
By being tested above it is found that product of the invention is safe and nontoxic.
Embodiment 4: flavor evaluation test
The reference product that following and product of the present invention is closer to is set:
Control 1: the products material of pawpaw composite enzyme, opposite embodiment 1 does not add dried orange peel, remaining raw material and preparation side Method while example 1.
Control 2: the products material of pawpaw composite enzyme, opposite embodiment 1 does not add salt, remaining raw material and preparation side Method while example 1.
Invite 50 rucks as judging panel altogether in this trial, using ten point system evaluation method, respectively to reality Apply a 1-2 and more than the products of 2 comparative experimental examples score, provide corresponding score, test process using Blind Test (i.e. Judging panel is not aware which product used).Specific evaluation result see the table below 1:
1 Analyses Methods for Sensory Evaluation Results of table
Serial number Group Sensory evaluation average mark The overall evaluation
1 Embodiment 1 8.97 Sour-sweet suitable, the light flowers and fruits fragrance of taste
2 Embodiment 2 8.85 Sour-sweet suitable, the light flowers and fruits fragrance of taste
3 Comparison 1 7.05 Taste is compared with sweet tea, British plain spirits
4 Comparison 2 6.43 Mouthfeel is poor, slight breakdown of fruit taste
The result tested by the above sensory evaluation is it is found that product of the invention compares the taste and flavor of comparison 1, comparison 2 Tool is greatly improved, and has reacted dried orange peel and salt meaning creative in the preparation of product of the present invention, and Therebetween there is also certain synergistic effect, the good dominant microflora and certain density salt of exactly dried orange peel offer are made For the pressure of strain existence so that most suitable dominant microflora has obtained maximized growth, thus obtain good mouthfeel and Flowers and fruits fragrance, and also the reconciliation of exactly salt and rock sugar receives consumption so that fermented product sour-sweet taste of the invention is suitable The welcome of person.
Embodiment 5: edible test
On the basis of safety testing, product of the invention has carried out foretasting test in a certain range, foretastes examination It tests and mainly has chosen 10 female constipation patients, suggest that it eats product 5g of the invention daily, deactivated after adhering to 10 days, observation Thereafter the defecation situation in 15 days.According to reflection, wherein 9 persons of foretasting arrange with still being able to daily normal amount in 15 days after deactivating Just, no longer there is the case where constipation, 1 person of foretasting starts the symptom for continuing constipation occur on the 12nd day after deactivating, through again Constipation symptom disappears after eating product of the invention.Through to 10 the person's of foretasting it has been observed that user is opposite take before, gas Blood is more unobstructed, of pink and healthy-looking, and have part user react sleep and halitosis all obtained good improvement, express willingness after It is continuous to take product of the invention.
In addition, being tested through inventor, 50g is taken daily, adheres to taking 10 days, diarrhea phenomenon does not during which occur, Also without any adverse reaction.
Disclosed above is only specific embodiments of the present invention, but the present invention is not limited to this, the skill of any this field What art personnel can think variation, should all fall within the scope and spirit of the invention.

Claims (5)

1. a kind of composite pawpaw glycoprotein ferment, which is characterized in that be made of the primary raw material of following parts by weight: pawpaw skin 50-60 weight Part, pawpaw seeds 5-10 parts by weight, dried orange peel 5-10 parts by weight, fruit of glossy privet 2-5 parts by weight, semen ziziphi spinosae 3-5 parts by weight, roselle 5-7 Parts by weight, rock sugar 35-40 parts by weight, salt 3-5 parts by weight;
The production method of the composite pawpaw glycoprotein ferment includes the following steps:
(1) pawpaw skin, pawpaw seeds, dried orange peel, the fruit of glossy privet, semen ziziphi spinosae, roselle, rock sugar and salt are weighed by weight;
(2) weighed pawpaw seeds are smashed using beater and is slurried, filtrate A is then obtained after filter and remove residue, it is spare;It will be weighed Rock sugar is clayed into power spare;
(3) fruit of glossy privet, semen ziziphi spinosae mixing will be weighed, 5-8 times of mixture quality of water is added, is heated to boiling in closed container And boiling 20-25 minutes is kept, cooled and filtered obtains liquor B;
(4) filtrate A, step (3) made from step (2) are added in the pawpaw skin cleaning weighed pawpaw skin, and cleaning backward Liquor B, weighed dried orange peel, roselle, salt and smashed rock sugar powder obtained, stir evenly, and are placed on 25-27 It ferments 8-10 days in the environment of degree Celsius, stirring stirring is primary within during which every 2 days, so that fermenting mixture be made;
(5) high-speed stirred is carried out to fermenting mixture using blender, so that slurries be made, institute is obtained after homogenizer homogeneous The composite pawpaw glycoprotein ferment stated;
The composite pawpaw glycoprotein ferment is added to dried orange peel by the way of natural fermented, has certain stable advantage zymophyte Group, in conjunction with the natural flora in pawpaw epidermis face, under the pressure condition and fermentation temperature control condition of certain brine concentration, so that Dominant bacteria including saccharomycete, lactic acid bacteria can mushroom out, to form effective fermentation.
2. composite pawpaw glycoprotein ferment according to claim 1, which is characterized in that be made of the primary raw material of following parts by weight: 55 parts by weight of pawpaw skin, 8 parts by weight of pawpaw seeds, 8 parts by weight of dried orange peel, 3 parts by weight of the fruit of glossy privet, 4 parts by weight of semen ziziphi spinosae, roselle 6 Parts by weight, 35 parts by weight of rock sugar, 4 parts by weight of salt.
3. composite pawpaw glycoprotein ferment according to claim 1, which is characterized in that be made of the primary raw material of following parts by weight: 58 parts by weight of pawpaw skin, 5 parts by weight of pawpaw seeds, 7 parts by weight of dried orange peel, 4 parts by weight of the fruit of glossy privet, 5 parts by weight of semen ziziphi spinosae, roselle 7 Parts by weight, 40 parts by weight of rock sugar, 5 parts by weight of salt.
4. composite pawpaw glycoprotein ferment according to claim 1, which is characterized in that use for convenience, further by step (5) composite pawpaw glycoprotein ferment made from carries out vacuum freeze drying, and composite pawpaw glycoprotein ferment powder is obtained after crushing, yet further should Composite pawpaw glycoprotein ferment powder is made capsule or is pressed into tablet or the form of tea bag is made.
5. a kind of method for producing any one of claim 1-3 composite pawpaw glycoprotein ferment, which is characterized in that including walking as follows It is rapid:
(1) pawpaw skin, pawpaw seeds, dried orange peel, the fruit of glossy privet, semen ziziphi spinosae, roselle, rock sugar and salt are weighed by weight;
(2) weighed pawpaw seeds are smashed using beater and is slurried, filtrate A is then obtained after filter and remove residue, it is spare;It will be weighed Rock sugar is clayed into power spare;
(3) fruit of glossy privet, semen ziziphi spinosae mixing will be weighed, 5-8 times of mixture quality of water is added, is heated to boiling in closed container And boiling 20-25 minutes is kept, cooled and filtered obtains liquor B;
(4) filtrate A, step (3) made from step (2) are added in the pawpaw skin cleaning weighed pawpaw skin, and cleaning backward Liquor B, weighed dried orange peel, roselle, salt and smashed rock sugar powder obtained, stir evenly, and are placed on 25-27 It ferments 8-10 days in the environment of degree Celsius, stirring stirring is primary within during which every 2 days, so that fermenting mixture be made;
(5) high-speed stirred is carried out to fermenting mixture using blender, so that slurries be made, institute is obtained after homogenizer homogeneous The composite pawpaw glycoprotein ferment stated;
The composite pawpaw glycoprotein ferment is added to dried orange peel by the way of natural fermented, has certain stable advantage zymophyte Group, in conjunction with the natural flora in pawpaw epidermis face, under the pressure condition and fermentation temperature control condition of certain brine concentration, so that Dominant bacteria including saccharomycete, lactic acid bacteria can mushroom out, to form effective fermentation.
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CN106360701A (en) * 2016-10-14 2017-02-01 广东盆地号生物产业有限公司 Roselle enzyme and preparing method thereof
CN109874897A (en) * 2019-04-03 2019-06-14 海南酵老师生物科技有限公司 A kind of Noni fruit fermentation fruit tea and preparation method thereof

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CN102233018A (en) * 2010-04-20 2011-11-09 李晓青 Pawpaw leaven of carica papaya and rosaceae plants as well as preparation method and application thereof
CN104962529A (en) * 2015-06-19 2015-10-07 广西大学 Method for manufacturing fruit ferment
CN105029432A (en) * 2015-06-22 2015-11-11 谢嘉源 Brewing technology of composite enzyme using chaenomeles sinensis as main material
CN105124579A (en) * 2015-09-09 2015-12-09 南宁荣港生物科技有限公司 Preparation method of compound liquid enzymes
CN105166898A (en) * 2015-09-09 2015-12-23 南宁荣港生物科技有限公司 Solid enzyme preparation method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102233018A (en) * 2010-04-20 2011-11-09 李晓青 Pawpaw leaven of carica papaya and rosaceae plants as well as preparation method and application thereof
CN104962529A (en) * 2015-06-19 2015-10-07 广西大学 Method for manufacturing fruit ferment
CN105029432A (en) * 2015-06-22 2015-11-11 谢嘉源 Brewing technology of composite enzyme using chaenomeles sinensis as main material
CN105124579A (en) * 2015-09-09 2015-12-09 南宁荣港生物科技有限公司 Preparation method of compound liquid enzymes
CN105166898A (en) * 2015-09-09 2015-12-23 南宁荣港生物科技有限公司 Solid enzyme preparation method

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