CN105639351A - Crocodile bone peptide drink capable of preventing and treating osteoporosis - Google Patents
Crocodile bone peptide drink capable of preventing and treating osteoporosis Download PDFInfo
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- CN105639351A CN105639351A CN201610029363.7A CN201610029363A CN105639351A CN 105639351 A CN105639351 A CN 105639351A CN 201610029363 A CN201610029363 A CN 201610029363A CN 105639351 A CN105639351 A CN 105639351A
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- crocodile
- bone peptide
- crocodile bone
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- osseocolla
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 89
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 81
- 208000001132 Osteoporosis Diseases 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 44
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- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 4
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- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
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- 241001465754 Metazoa Species 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 206010039984 Senile osteoporosis Diseases 0.000 description 2
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- SLAMLWHELXOEJZ-UHFFFAOYSA-N 2-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 description 1
- 206010000077 Abdominal mass Diseases 0.000 description 1
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- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 description 1
- 241000243684 Lumbricus Species 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 206010028347 Muscle twitching Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000025747 Rheumatic disease Diseases 0.000 description 1
- 241000447727 Scabies Species 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
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- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 150000001669 calcium Chemical class 0.000 description 1
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- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
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- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- FQTCUKQMGGJRCU-UHFFFAOYSA-N n,n-diacetylacetamide Chemical compound CC(=O)N(C(C)=O)C(C)=O FQTCUKQMGGJRCU-UHFFFAOYSA-N 0.000 description 1
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- 239000001509 sodium citrate Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention creatively provides a crocodile bone peptide drink capable of preventing and treating osteoporosis. The crocodile bone peptide drink is prepared from crocodile bone peptide, fructus chaenomelis lagenariae, Chinese wolfberry fruits and purified water. Compared with the prior art, the crocodile bone peptide drink has the advantages that crocodile bone peptide is adopted as the main raw material, and such Chinese herbal medicine as fructus chaenomelis lagenariae and Chinese wolfberry fruits is added, so that the compound crocodile bone peptide drink is processed and obtained, all effects supplement with one another, the human body can be helped to better absorb crocodile bone peptide, the efficacy of crocodile bone peptide can be achieved to the greatest extent, the production method is simple and easy to conduct, and if the crocodile bone peptide drink is drunk frequently, osteoporosis can be prevented and treated.
Description
Technical field
The invention belongs to health product technology field, particularly to a kind of crocodile bone peptide health promoting beverage with good prevention, treatment osteoporosis effect being main component with crocodile bone Gly-His-Lys.
Background technology
Crocodile is the most ancient reptile, has survived on earth more than 200,000,000 3 thousand ten thousand years, thus has had the title of " living fossil ". Crocodile Gu claims Chinese alligator dragon, Lumbricus, and Compendium of Material Medica once recorded its first acrid in the mouth, warm in nature, has removing blood stasis by becoming silted up, effect of detumescence and promoting granulation. " China's book on Chinese herbal medicine " the 26th volume load: the first sheet of Chinese alligator (Alligatorsinensis), medicine name Squama Alligator, has blood stasis dispelling, removing food stagnancy, insecticidal function, cures mainly abdominal mass and gather, metrorrhagia leukorrhagia, skin ulcer scabies, malignant boil, hemorrhoidal hamorrhage haemorrhoids.
Crocodile bone has the function such as strengthening the tendons and bones, life essence-filling, marrow-benefitting, and rheumatic ostalgia is effective in cure. Mice is had the stress function and hematopoietic function by crocodile osseocolla, can significantly improve mice tolerance in high temperature and low temperature environment, and crocodile osseocolla has the effect of enhancing body the stress function and increased platelets counts. Research shows, crocodile bone meal contains the materials such as substantial amounts of activated calcium and phosphorus, can be used for preventing and treating senile osteoporosis, children's's rickets etc. Therefore, the crocodile bone peptide beverage that crocodile bone collagen peptide is main component can prevent and treat senile osteoporosis, it addition, crocodile bone peptide and Chinese medicine ingredients compatibility can strengthen it treats osteoporotic effect.
Summary of the invention
In view of this, the invention is directed to a kind of crocodile bone peptide beverage with prevention and treatment osteoporosis, to meet the daily health caring demand of people.
For reaching above-mentioned purpose, the technical scheme of the invention is achieved in that
A kind of crocodile bone peptide beverage with prevention and treatment osteoporosis, the raw material of described crocodile bone peptide beverage comprises crocodile bone peptide, Fructus Chaenomelis, Fructus Lycii, pure water.
Further, to comprise following weight parts composed as follows for described raw material: crocodile bone peptide 0.5-2 part, Fructus Chaenomelis 5-10 part, Fructus Lycii 5-15 part, pure water 1000-2000 part.
Further, described crocodile bone peptide beverage has effect of prevention and releasing osteoporosis disease.
The another object of the invention is in that to propose the extracting method of a kind of crocodile bone peptide, and described extracting method comprises the steps of: (1) takes crocodile bone and scrapes the meat muscle of attachment on os purum, and boiling water steams 30��40min;
(2) steamed crocodile bone in step (1) is thinly sliced, after adding the NaOH solution immersion 4��6h of 0.05mol/l, be washed till neutrality with pure water;
(3), after then adding the sulfuric acid solution immersion 4��6h of 0.2%, it is washed till neutrality with pure water;
(4) by the crocodile osteocomma hot-water extraction after soda acid soaks and cleans, discarding precipitation and insoluble matter, obtain crocodile osseocolla supernatant, concentration obtains the thick glue goods of crocodile osseocolla after being evaporated;
(5) weigh the thick glue goods of crocodile osseocolla and add neutral protease enzymolysis;
(6) the crocodile osseocolla crude product after enzymolysis is inactivated in boiling water bath, centrifugal, take supernatant and obtain crocodile osseocolla polypeptide solution.
Further, the hot-water extraction condition in described step (4) is the centrifugal 20��30min that is placed in hot water by crocodile osteocomma 4000��6000rpm.
Further, the enzymatic hydrolysis condition in described step (5) is hydrolysis 5h in 45 DEG C of water-baths.
Further, the inactivation condition in described step (6) is inactivation 20min in boiling water bath, and centrifugal condition is centrifugal 20min.
The preparation method that present invention additionally comprises a kind of crocodile bone peptide beverage, described method is:
(1) weigh the Fructus Chaenomelis of formula ratio, Fructus Lycii water carries 2��3 times, merging filtrate after filtration, and precipitation refilters, and obtains supernatant;
(2) take supernatant in step (1) and the crocodile osseocolla polypeptide solution mixing prepared, add the pure water of formula ratio and be configured to subpackage, sterilizing after described crocodile bone peptide beverage.
Further, it is each 2 hours that the water in described step (1) puies forward process, and it is 8 hours that water carries the sedimentation time after filtration.
Further, the sterilized environment in described step (2) is 121 DEG C, 0.11MPa sterilizing 30min.
Relative to prior art, the crocodile bone peptide beverage described in the invention has the advantage that
Crocodile bone peptide beverage of the present invention adopts crocodile bone peptide to be primary raw material, and add the compound recipe crocodile bone peptide beverage that the Chinese herbal medicine such as Fructus Chaenomelis, Fructus Lycii is processed into, various effects are complementary, human body can be helped better to absorb crocodile bone peptide, the effect making crocodile bone peptide obtains maximum performance, manufacture method is simple, often drinks and can prevent and treat osteoporosis.
Detailed description of the invention
Unless otherwise indicated, term used herein is respectively provided with the implication that those skilled in the art's routine is understood, and for the ease of understanding the present invention, terms more used herein has been carried out following definitions.
Using in the specification and in the claims, odd number type " " and " this " include plural reference, clearly state unless the context otherwise. Such as, term " (one) cell " includes the cell of plural number, including its mixture.
All of Digital ID, for instance pH, temperature, time, concentration, including scope, is all approximation. It is to be understood that, although all add term " about " before all of Digital ID of always not clear and definite narration. Simultaneously it will also be understood that, although always not clear and definite narration, reagent described herein is only example, and its equivalent is known in the art.
Effect of the crocodile bone peptide beverage of the invention is described in detail below in conjunction with embodiment and different experiments example and coherent detection data.
Wherein, in embodiment, test material used and source thereof include:
1. animal:
Male mouse of kunming 20, body weight 30��35g, to raise in SPF rank Mice Residence, laboratory animal is provided by National Institute for Food and Drugs Control, the quality certification number: NO.11400500007331.
2. Experimental agents and reagent
Crocodile bone peptide beverage: liquid, is provided by Bai Shengkang bio tech ltd, Beijing.
Retinoic acid (retinoic acid): Shanghai Sheng Gong biological engineering company limited, article No.: A506728, lot number: 302-79-4, purity: > 98%.
EDTA: purchased from Shanghai Sheng Gong biological engineering company limited, article No.: A100105, lot number: 6381-92-6, rank: analytical pure, specification 500g.
Reduced glutathion (GSH): purchased from Shanghai Sheng Gong biological engineering company limited, article No.: A100399, lot number: 70-18-8, purity: > 98%, specification 5g.
5,5 '-dithio double; two (2-nitrobenzoic acid): purchased from Shanghai Sheng Gong biological engineering company limited, lot number: 69-78-3, specification: 5g.
3. key instrument
Ultraviolet spectrophotometer: Japan's Shimadzu, model: UV-2201;
Electronic balance: Shanghai Ao Haosi Instrument Ltd., model: AR2202CN;
4. the compound method of agents useful for same includes:
Hydrazoic acid,sodium salt-phosphate buffer (PH7.0): Hydrazoic acid,sodium salt 16.3mg, disodium hydrogen phosphate 7.16g, sodium dihydrogen phosphate 3.12g, EDTA7.4mg, distilled water dissolves and is diluted to nearly 100ml, be adjusted to PH7.0 by the NaOH solution of 4.8mol/l, last constant volume in 100ml measuring bottle, Refrigerator store.
1.0mmol/LGSH solution: weigh GSH3.07mg, dissolves with Hydrazoic acid,sodium salt-phosphate buffer and is surely dissolved in 10ml measuring bottle, prepared before use.
1.0mmol/LDTNB nitrite ion: weigh DTNB20mg, trisodium citrate 500mg, dissolves with distilled water and constant volume is with 50ml measuring bottle, and brown bottle keeps in Dark Place in 4 DEG C of refrigerators.
Embodiment 1
A kind of crocodile bone peptide beverage with prevention and treatment osteoporosis, described crocodile bone peptide beverage is mainly by the component of following weight portion: crocodile osseocolla polypeptide 0.5 part, Fructus Chaenomelis 6 parts, Fructus Lycii 10 parts, 2000 parts of allotments of purified water form.
The preparation method of described crocodile bone peptide beverage is: first crocodile bone shaves the meat muscle of attachment, and boiling water steams 30min, shave, and the NaOH solution adding 0.05mol/l soaks 4h, and pure water is washed till neutrality. Adding 0.2% sulfuric acid solution again and soak 4h, pure water be washed till neutrality, by hot-water extraction, 4000rpm is centrifuged 20min, discards precipitation insoluble matter, obtains supernatant concentration and is evaporated and obtains the thick glue goods of crocodile osseocolla. Weigh the thick glue goods of crocodile osseocolla, add appropriate neutral protease, after 45 DEG C of water-baths are hydrolyzed 5h, boiling water bath inactivate 20min, centrifugal 20min, takes supernatant and be crocodile osseocolla polypeptide solution. Separately take Fructus Chaenomelis, Fructus Lycii water carries 2 times, each 2 hours, filters, merging filtrate, precipitates 8 hours, and after filtration, gained supernatant mixes with crocodile osseocolla polypeptide solution, adds the purified water preparation of formula ratio, subpackage 100ml/ bottle, 121 DEG C, 0.11MP sterilizing 30min.
The research of the treatment osteoporosis function of experimental example 1 crocodile bone peptide beverage
One, experimental technique:
1. the foundation of mice retinoic acid osteoporosis model and crocodile bone peptide beverage are fed
(1) foundation of retinoic acid osteoporosis model
20 male mouse of kunming prepare to set up osteoporosis model after SPF level receptacle diet stablizes three days, and 20 mices are randomly divided into three groups, respectively normal group, model group, crocodile bone peptide solution group and crocodile bone peptide beverage group. Except normal group, the retinoic acid that its excess-three group gavage every day gives 150mg/kg sets up osteoporosis model, and the modeling time continues two weeks.
(2) nursing of crocodile bone peptide beverage
After the osteoporosis model of Induced by Retinoic Acid has been set up, start to give crocodile bone peptide beverage to feed, wherein, normal group and model group mice freer drinking-water every day except normal diet, crocodile bone peptide solution group (contained crocodile bone peptide content is consistent to beverage) and crocodile bone peptide beverage group give the corresponding beverage of 7.5ml/20g volume respectively every day and feed except drinking water except normal diet, the time of feeding continues three weeks.
2. mouse femur volume and calcium content of bone measure
(1) measurement in mouse femur volume and long and short footpath
Feeding crocodile bone peptide beverage, after 21 days, puts to death mice, peels off mouse femur, it is placed in baking oven dry, weighs dried mouse femur weight, record bone tuple evidence, femur dry weight is designated as B, is placed in by mouse femur in 1ml syringe subsequently, promotes nook closing member, femur is supported to syringe, then syringe is placed in the water of 4 DEG C, twitches nook closing member, until femur is immersed in water, stir syringe to be discharged by air simultaneously, promote nook closing member to make it prop up femur. Carry out labelling at syringe nook closing member boundary, be designated as L by the distance bottom vernier caliper measurement nook closing member to syringe, then weigh syringe gross weight (in syringe only moisture and bone), be recorded as C. Extracting nook closing member out, femur and water are removed syringe, then draws water to the scale place of labelling, and blotted by the water around syringe with filter paper, weigh, record syringe and water are heavily D. Femur is shared volume V=D+B-C in syringe, according to water volume weight conversion method in 4 DEG C of water: the volume of 1g water is 1cm3, femur weight can be scaled volume. The femur vernier caliper measurement taken out its grow crosswise footpath and horizontal minor axis, record result (detailed results is in Table 1).
Table 1 mouse femur volume and the change of long and short footpath meterological
(2) bone calcium is measured
Take mouse femur, after 10%HCl soaks 7 days for tri-times, collect soak 15ml, 15ml soak is diluted to 100ml. Take wherein 30ml soak and be placed in conical flask, it is added thereto to 1ml triacetamide and 5ml ammonia buffer, then being neutralized to PH by the NaOH solution of 0.005mol/L is 6.5��7, instill 2��3 black T of network as indicator, it is blue for starting to be titrated to solution with the EDTA standard solution of 0.01mol/L, the volume of record now EDTA solution used. Calcium content (mg)=C in soakEDTA��VEDTA��MCa(calcium atom amount) �� 100/30, record result of calculation (referring to table 2).
Table 2 mouse femur calcium content of bone meterological change (x �� s)
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3. GSHPx content and tissue GSH assay in Mouse whole blood
(1) Mouse whole blood GSHPx assay
Crocodile bone peptide beverage is fed after terminating, and mice is carried out eyeball and takes blood, and every 50 �� l are standby.
GSH standard curve determination: take 1.0mMGSH standard solution 0,0.2,0.4,0.6,0.8 and 1.0ml respectively, then often manages each addition 8.0ml Metaphosphoric acid precipitated liquid, and uses ddH2O is settled to 10ml, to obtain the concentration respectively GSH titer of 0,20,40,60,80 and 100 ��M. The titer of variable concentrations is sequentially placed in cuvette, then all adds 2.5ml0.32MNa2HPO4After mixing with 0.5mlDTNB nitrite ion, measure the absorbance under 422nm wavelength with ultraviolet spectrophotometer, make GSH standard curve.
Take 10 �� l Mouse whole blood, use ddH2It is standby that O is diluted to 1.0ml. Sample cell is sequentially added into 0.4ml1.0mMGSH, 0.4ml diluting blood sample liquid, after 5min is hatched in 37 DEG C of water-baths, adds the H2O2 of 0.2ml preheating, continue water-bath 3min, after adding 4.0ml Metaphosphoric acid precipitated liquid, 3000rpm, centrifugal 10min, abandons precipitation and takes 2ml supernatant, add 2.5ml0.32MNa2HPO4After mixing with 0.5mlDTNB nitrite ion, measure the absorbance under 422nm wavelength with ultraviolet spectrophotometer.As blank determination non-enzymatic pipe absorbance, except using 0.4mlddH2O replaces diluting blood sample liquid all the other components outer consistent with sample cell. Also needing as blank to measure one group of blank tube, blank tube only adds 0.4mlddH2O, 1.6ml Metaphosphoric acid precipitated liquid, 2.5ml0.32MNa2HPO4With 0.5mlDTNB nitrite ion. Three groups measured after calculate serum SOD Px unit of activity number. Mus serum SOD Px unit of activity number=log (ODNon-enzymatic pipe-ODBlank tube)-log(ODSample cell-ODBlank tube)/3min/0.004ml (result is in Table 3).
GSHPx meterological change (x �� s) in table 3 Mouse whole blood
_
(2) mouse tissue GSH assay
Take tissue liver, kidney and heart after sacrifice, measure wherein GSH content. Each tissue rejects fat and connective tissue after cleaning with pre-cooling volume normal saline, blot with filter paper, on ice, tissue is shredded, with 9ml0.25M sucrose-0.01MTris buffer by 1g tissue homogenate, the centrifugal 30min of 8000g is changed after the centrifugal 20min of 500g, take tissue supernatant, also according to DTNB Direct Determination GSH content (result is in Table 4).
Table 4 mice respectively organize in GSH content meterological change (x �� s) (mol/g wet tissue)
Two, interpretation
1. as shown in table 1, the femur volume of osteoporosis model group mice and grow crosswise, minor axis is significantly less than normal group, and the mouse femur volume of crocodile bone peptide solution group and crocodile bone peptide beverage group and grow crosswise, minor axis relatively model group increases all to some extent, but crocodile bone peptide beverage group is substantially better than crocodile bone peptide solution group.
2. as shown in table 2, the bone calcium content of femur of osteoporosis model group mice decreases compared to normal group, and after drinking crocodile bone peptide beverage, Mouse Bone calcium content gos up to some extent compared to model group, and crocodile bone peptide beverage group is substantially better than crocodile bone peptide solution group.
3. as shown in table 3, after retinoic acid modeling, the glutathion peroxidase level in model group Mouse whole blood and vigor are decreased obviously compared with normal group, drink crocodile bone peptide beverage than drinking crocodile bone peptide solution and more can promote content and the activity level of osteoporosis Mouse whole blood glutathion peroxidase body.
4. as shown in table 4, in osteoporosis model group mouse liver, kidney and heart tissue, all there is reduction in various degree than normal group in the content of reduced glutathion, after drinking crocodile bone peptide solution and crocodile bone peptide beverage, the reduced glutathion level in Mouse Liver, kidney and heart has raising in various degree compared to model group, but crocodile bone peptide beverage group improves degree and is substantially better than crocodile bone peptide solution group.
The foregoing is only the preferred embodiment of the invention; not in order to limit the invention; within all spirit in the invention and principle, any amendment of making, equivalent replacement, improvement etc., should be included within the protection domain of the invention.
Claims (10)
1. a crocodile bone peptide beverage with prevention and treatment osteoporosis, it is characterised in that: the raw material of described crocodile bone peptide beverage comprises crocodile bone peptide, Fructus Chaenomelis, Fructus Lycii, pure water.
2. crocodile bone peptide beverage according to claim 1, it is characterised in that: it is composed as follows that described raw material comprises following weight parts: crocodile bone peptide 0.5-2 part, Fructus Chaenomelis 5-10 part, Fructus Lycii 5-15 part, pure water 1000-2000 part.
3. crocodile bone peptide beverage according to claim 1, it is characterised in that: described crocodile bone peptide beverage has effect of prevention and releasing osteoporosis disease.
4. the extracting method of crocodile bone peptide according to claim 1, it is characterised in that described extracting method comprises the steps of: (1) takes crocodile bone and scrapes the meat muscle of attachment on os purum, and boiling water steams 30��40min;
(2) steamed crocodile bone in step (1) is thinly sliced, after adding the NaOH solution immersion 4��6h of 0.05mol/l, be washed till neutrality with pure water;
(3), after then adding the sulfuric acid solution immersion 4��6h of 0.2%, it is washed till neutrality with pure water;
(4) by the crocodile osteocomma hot-water extraction after soda acid soaks and cleans, discarding precipitation and insoluble matter, obtain crocodile osseocolla supernatant, concentration obtains the thick glue goods of crocodile osseocolla after being evaporated;
(5) weigh the thick glue goods of crocodile osseocolla and add neutral protease enzymolysis;
(6) the crocodile osseocolla crude product after enzymolysis is inactivated in boiling water bath, centrifugal, take supernatant and obtain crocodile osseocolla polypeptide solution.
5. the extracting method of crocodile bone peptide according to claim 4, it is characterised in that: the hot-water extraction condition in described step (4) is the centrifugal 20��30min that is placed in hot water by crocodile osteocomma 4000��6000rpm.
6. the extracting method of crocodile bone peptide according to claim 4, it is characterised in that: the enzymatic hydrolysis condition in described step (5) is hydrolysis 5h in 45 DEG C of water-baths.
7. the extracting method of crocodile bone peptide according to claim 4, it is characterised in that: the inactivation condition in described step (6) is inactivation 20min in boiling water bath, and centrifugal condition is centrifugal 20min.
8. the preparation method of crocodile bone peptide beverage according to claim 1, it is characterised in that described method is:
(1) weigh the Fructus Chaenomelis of formula ratio, Fructus Lycii water carries 2��3 times, merging filtrate after filtration, and precipitation refilters, and obtains supernatant;
(2) take supernatant in step (1) and the crocodile osseocolla polypeptide solution mixing prepared, add the pure water of formula ratio and be configured to subpackage, sterilizing after described crocodile bone peptide beverage.
9. the preparation method of crocodile bone peptide beverage according to claim 8, it is characterised in that it is each 2 hours that the water in described step (1) puies forward process, it is 8 hours that water carries the sedimentation time after filtration.
10. the preparation method of crocodile bone peptide beverage according to claim 8, it is characterised in that the sterilized environment in described step (2) is 121 DEG C, 0.11MPa sterilizing 30min.
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