CN114191499B - Traditional Chinese medicine composition for relieving liver and kidney injury - Google Patents

Traditional Chinese medicine composition for relieving liver and kidney injury Download PDF

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CN114191499B
CN114191499B CN202210106893.2A CN202210106893A CN114191499B CN 114191499 B CN114191499 B CN 114191499B CN 202210106893 A CN202210106893 A CN 202210106893A CN 114191499 B CN114191499 B CN 114191499B
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extract
chinese medicine
traditional chinese
cistanche
yam
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CN114191499A (en
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邢炎华
侯少平
杨敏
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Shaanxi Institute of International Trade and Commerce
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/64—Orobanchaceae (Broom-rape family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/80—Scrophulariaceae (Figwort family)
    • A61K36/804—Rehmannia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a traditional Chinese medicine composition for relieving liver and kidney injury and application thereof, wherein the traditional Chinese medicine composition consists of a traditional Chinese medicine extract and vitamin C, and the traditional Chinese medicine extract comprises the following components in parts by volume: 100-300 parts of cistanche extract, 100-300 parts of yam extract and 12-25 parts of rehmannia root extract; the cistanche extract, the yam extract and the rehmannia root extract are all water extracts; the ratio of the traditional Chinese medicine composition to the vitamin C is 10-50:1-2 in terms of mL/mg. The components of the formula are mutually cooperated, the prevention or alleviation effect on kidney and/or liver injury can be fully exerted, the product is high in safety and free of any toxic and side effects, and the product is an optimal nutritional health care product suitable for preventing and alleviating kidney and liver injury.

Description

Traditional Chinese medicine composition for relieving liver and kidney injury
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, relates to a traditional Chinese medicine compound health food, and in particular relates to a traditional Chinese medicine composition for relieving liver and kidney injury.
Background
Alcoholic liver injury (Alcoholic Liver Disease, ALD) is a complex metabolic disease caused by long-term overdrinking, a second liver disease that severely jeopardizes human health following viral hepatitis. Alcohol metabolites such as acetaldehyde, acetic acid and free radicals cause extensive oxidative damage to important organ tissues of the body such as the liver, kidneys and spleen. Excessive drinking mainly causes damage to liver, and if the disease condition is continuously developed, alcoholic hepatitis, alcoholic liver fibrosis, alcoholic liver cirrhosis and even liver cancer may be caused. Ethanol can also be metabolized by the kidneys, and excessive drinking causes acetaldehyde, free radicals and the like to accumulate in kidney cells and kidney interstitium, surpassing the metabolic load thereof, resulting in kidney injury, including renal nipple necrosis, infectious glomerulonephritis and acute renal failure. Alcohol can cause immune system disorder, resulting in spleen cell permeability enhancement, and thus, body immunity reduction. Excessive intake of wine can also damage the mesentery, cause greater mesenteric permeability, dysbacteriosis, endotoxin contact the liver, and cause liver inflammatory response.
Fructose is a hexose, an isomer of glucose, widely found in sugar cane, sugar beet, honey and most fruits, and is the sweet one of the monosaccharides. Along with the breakthrough of the industrial production technology of fructose, more and more fructose is widely used in various food additives, so that people ingest more fructose in daily life. At present, a plurality of clinical cases and experimental researches show that the great intake of fructose is related to factors such as blood uric acid rise, gouty nephropathy, insulin resistance metabolic syndrome and the like. And many clinical and experimental animal studies have found that intake of high fructose, either at one time or over a long period of time, results in elevated serum uric acid levels.
Cistanche salsa (Cistanche deserticola Ma), named Jiangyun, cunyun, cistanche salsa, charpy, etc., belongs to an endangered species. Cistanche deserticola belongs to tall herb, is 40-160 cm in height, and is mostly grown underground. Cistanche is mainly produced in Xinjiang, the inner Mongolia and the Allen, gansu and Ningxia are distributed. Modern pharmacological researches show that the phenylethanoid glycosides compounds acteoside contained in cistanche deserticola have the function of regulating the neuroendocrine system; d-mannitol and cistanche polysaccharide in cistanche deserticola can obviously increase spleen quality of mice and enhance cellular immunity; the cistanche deserticola extract can obviously reduce the content of Lipid Peroxide (LPO) in human bodies, and has obvious inhibition effect on the LPO generation of rabbit blood and mouse livers; the cistanche can prolong the swimming time of the mice, reduce the rise amplitude of serum creatine kinase after load exercise, and keep the ultrastructure of skeletal muscle of the mice normal after exercise, which indicates that the cistanche has the functions of enhancing physical strength and resisting fatigue; cistanche deserticola has effects of scavenging free radicals and resisting lipid peroxidation, and has anti-hepatitis effect. The scholars at home and abroad conduct extensive pharmacological activity research on cistanche deserticola, and find that the active ingredients of cistanche deserticola mainly comprise phenethyl alcohol glycoside and polysaccharide and mainly concentrate on cistanche deserticola carried in pharmacopoeia of the people's republic of China, and the used samples are also crude extracts of traditional Chinese medicines.
The yam is a dried tuber of yam (Dioscorea opposita thunb) which is a perennial herb of the family Dioscoreaceae, and is widely distributed in hilly and shallow mountain areas such as northeast, north China, southeast and southwest in China, and also distributed in Korea and Japan, and forms a plurality of local varieties. The Chinese yam is divided into two major categories, namely edible and medicinal, and the edible Chinese yam is mainly used in the south provinces. The artificially planted Chinese yam has white meat color and sweet and powdery taste, is large in mass and hard in mass and is mostly eaten. The yam tuberous root contains rich starch, saponin, mucilage (mainly mannans, phytic acid and the like), choline, glycoprotein and various amino acids, and is a name for both medicine and food. The yam has the pharmacological effects of regulating immune function, improving digestion function, reducing blood sugar and blood fat, delaying aging, resisting tumor and mutation, promoting kidney regeneration and repair, and the like.
Rehmannia root is root tuber of rehmannia (Rehmannia glutinosa) belonging to the Scrophulariaceae, has the effects of nourishing yin, clearing heat, enriching blood and stopping bleeding, and is divided into dried rehmannia root, fresh rehmannia root and prepared rehmannia root, and the effects of the three are emphasized. The prior researches show that the main chemical components of the rehmannia root are iridoid glycoside, oligosaccharide, polysaccharide, amino acid and the like, and the rehmannia root is a substance basis for playing the drug effect of the rehmannia root. Rehmannia is widely used clinically, and has effects on immune, hematopoietic, cardiovascular and central nervous systems: 1. has bidirectional regulating effect on immune system: the fresh rehmannia root water extract can enhance spleen lymphocyte function of a yin deficiency model mouse, rehmannia root oligosaccharide can improve humoral and cellular immunity functions of a cyclophosphamide immunosuppressed mouse, and radix rehmanniae shows immunosuppression effect on a corticosterone yin deficiency model mouse; 2. enhancing hematopoietic function; 3. regulating blood pressure, protecting cardiovascular system: rehmannia root can reduce the ATPase activity of the heart and brain of a model rat, protect heart and brain tissues from ATP depletion and ischemia injury, and the Huai Di aqueous extract shows the functions of reducing acute hypertension and regulating blood pressure in two directions under cold conditions; 4. sedation, anti-aging: rehmannia root can inhibit the central nervous system to have sedative effect, and can inhibit lipid peroxidation and activities of NOS and SOD, regulate immunity and delay aging.
Vitamin C (Vitamin C, ascorbicacid) is L-ascorbic acid, and is a water-soluble Vitamin. Vitamin C in the food is absorbed by the upper segment of the human small intestine. Once absorbed, it is distributed to all water-soluble structures in the body, and there is about 1500mg of vitamin C in the vitamin C metabolic active pool in normal adult human body, and the maximum storage peak is 3000mg of vitamin C. Normally, most of vitamin C is decomposed into oxalic acid or combined with sulfuric acid in vivo by metabolism to generate ascorbic acid-2-sulfuric acid which is discharged from urine; the other part can be directly discharged from the body by urine. Vitamin C can enhance antibodies in human body, protect the activity of enzyme systems in human body, participate in detoxification, and promote wound healing and bone development. In recent years, it has been found that vitamin C has effects in preventing atherosclerosis, preventing common cold and iron deficiency anemia, improving stress ability, and resisting cancer.
Alcohol and fructose intake brings about damage to the kidneys and livers, and specific medicines for treating kidney and liver diseases are lacking, so that medicines clinically used at present are beneficial to delaying the onset of the kidneys and/or livers or have the potential of alleviating symptoms, but most medicines have certain side effects, and when the purpose of treating the livers is achieved, the dosage, the treatment course, the safety and the side effects of the medicines are still required to be further studied. Meanwhile, clinical medicines for treating kidney and/or liver injury can further increase risks of adverse reactions in cardiovascular and digestive tracts and the like, and have certain side effects, and proper functional foods are not available in the market. Therefore, the functional Chinese herbal compound health food is developed to be suitable for preventing and relieving kidney and liver injury, and has great development significance and market demand.
Disclosure of Invention
The invention aims to develop a functional traditional Chinese medicine compound health food capable of preventing or relieving kidney and/or liver injury.
Based on the above-mentioned objects, the present invention provides a traditional Chinese medicine composition for alleviating liver and kidney injury to solve the need in the art.
In one aspect, the invention relates to a traditional Chinese medicine composition for relieving liver and kidney injury, which comprises a traditional Chinese medicine extract and vitamin C; the traditional Chinese medicine extract comprises the following components in parts by volume: 100-300 parts of cistanche extract, 100-300 parts of yam extract and 12-25 parts of rehmannia root extract; the cistanche extract, the yam extract and the rehmannia root extract are all water extracts; the ratio of the traditional Chinese medicine extract to the vitamin C is 10-50:1-2 in terms of mL/mg.
Further, in the traditional Chinese medicine composition provided by the invention, the preparation method of the cistanche extract or the yam extract or the rehmannia root extract comprises the following steps: pulverizing cistanche, yam or rehmannia root, sieving, extracting with water at 35-40 deg.C under ultrasonic condition for 2-4 times, extracting for 1-2 hr each time, and mixing the extracting solutions.
Further, in the traditional Chinese medicine composition provided by the invention, the steps of concentrating and removing sediment are further included before the extracting solutions are combined; in the preparation method of the cistanche extract, the concentration is that the ratio of the concentrated solution of the combined extract to the cistanche is (1-1.5) 1 in terms of mL g; in the preparation method of the yam extract, the concentration is that the ratio of the concentrated extract liquid after combination to the yam is (2.5-3) 1 in terms of mL g; in the preparation method of the rehmannia root extract, the concentration is that the ratio of the concentrated extract liquid after combination to the rehmannia root is (4-4.5) 1 in terms of mL/g.
The cistanche extract, the yam extract, the rehmannia extract and the vitamin C are compounded, and the prepared traditional Chinese medicine composition has excellent effect in preventing or relieving kidney and/or liver injury. The food containing the traditional Chinese medicine composition as an effective component achieves the aim of relieving the damage of the kidney and/or liver caused by alcohol or fructose. The invention thus further claims the use of said Chinese medicinal composition for the preparation of a health food for preventing or alleviating kidney and/or liver damage induced by the intake of chemicals comprising alcohol and fructose. Based on the application, the invention further claims the traditional Chinese medicine composition as a health food and a health food for preventing or relieving kidney and/or liver injury.
Compared with the prior art, the invention has the beneficial effects or advantages that:
the invention provides a traditional Chinese medicine composition for relieving liver and kidney injury, which comprises effective components of traditional Chinese medicine extracts and vitamin C, wherein the traditional Chinese medicine extracts comprise cistanche extract, yam extract and rehmannia root extract. The inventor conducts a pharmacodynamic test on the traditional Chinese medicine composition, and discovers that the traditional Chinese medicine composition is better than the single use effect of each component in preventing or relieving kidney and/or liver injury within a certain proportioning range, and shows that each component has a coordination and synergy effect.
Drawings
FIG. 1 shows the body weight change of rats after alcohol contamination.
FIG. 2 shows liver coefficients of rats after alcohol contamination.
FIG. 3 shows kidney coefficients of rats after alcohol challenge.
FIG. 4 shows ALT and AST activity in serum after alcohol exposure of rats.
FIG. 5 shows the GLU and BUN concentrations in serum after alcohol exposure in rats.
FIG. 6 shows CREA concentration in serum after alcohol exposure of rats.
Fig. 7 shows XO and ADA levels in liver after fructose exposure in rats.
Fig. 8 is MDA concentration in kidneys after fructose exposure in rats.
FIG. 9 shows SOD concentration in kidneys after fructose exposure in rats.
Figure 10 shows CAT concentration in kidneys after fructose exposure in rats.
In the above figures, group 1 is a blank control group; group 2 is a model control group, and the modeling is alcohol contamination or fructose contamination; group 3-6 is single dose group, group 3 is cistanche extract, group 4 is yam extract, group 5 is rehmannia extract, group 6 is vitamin C; groups 7 to 9 are groups of the traditional Chinese medicine extracts, group 7 is the traditional Chinese medicine extract shown in example 1, group 8 is the traditional Chinese medicine extract shown in example 2, and group 9 is the traditional Chinese medicine extract shown in example 3; groups 10 to 12 are test groups, group 10 is the Chinese medicinal composition shown in example 1, group 11 is the Chinese medicinal composition shown in example 2, and group 12 is the Chinese medicinal composition shown in example 3.
Detailed Description
The following describes the technical aspects of the present invention with reference to examples, but the present invention is not limited to the following examples.
Example 1
The embodiment provides a preparation test of a traditional Chinese medicine composition for relieving liver and kidney injury.
The cistanche deserticola used in the implementation is cistanche deserticola, and the origin is the Arena alliance of the inner Mongolia autonomous region; the yam is iron stick yam, the cultivation soil is a soil, and the production area is a city, a county and a temperature county of the Coke of Henan province; the rehmannia root is produced in Henan province and Jiaozhen city and Shang-Feng county.
Extract of traditional Chinese medicine: 100 parts of cistanche extract, 100 parts of yam extract and 12 parts of rehmannia root extract are mixed in parts by volume. 1mg of vitamin C is added into each 10mL of the traditional Chinese medicine composition, and the traditional Chinese medicine composition is obtained after uniform mixing.
The preparation method of cistanche extract comprises the following steps: weighing 50g of cistanche deserticola, crushing, sieving with a 150-mesh sieve, adding deionized water with 10 times of mass, and extracting for 2 hours at 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 50mL, centrifuging to remove precipitate, and obtaining herba cistanches extract.
The preparation method of the yam extract comprises the following steps: weighing 50g of Chinese yam, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 5 times that of the powder, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 125mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
The preparation method of the rehmannia root extract comprises the following steps: weighing 50g of rehmannia root, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 10 times that of the rehmannia root, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding 10 times of deionized water for the second time, and extracting at 40 ℃ for 2 hours by ultrasonic treatment; mixing the two extractive solutions, concentrating to 200mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
Example 2
The embodiment provides a preparation test of a traditional Chinese medicine composition for relieving liver and kidney injury.
The cistanche deserticola used in the implementation is cistanche deserticola, and the origin is the Arena alliance of the inner Mongolia autonomous region; the yam is iron stick yam, the cultivation soil is a soil, and the production area is a city, a county and a temperature county of the Coke of Henan province; the rehmannia root is produced in Henan province and Jiaozhen city and Shang-Feng county.
Extract of traditional Chinese medicine: 200 parts of cistanche extract, 200 parts of yam extract and 18 parts of rehmannia root extract are mixed according to the volume parts. 1.5mg of vitamin C is added into each 30mL of the traditional Chinese medicine composition, and the traditional Chinese medicine composition is obtained after uniform mixing.
The preparation method of cistanche extract comprises the following steps: weighing 50g of cistanche deserticola, crushing, sieving with a 150-mesh sieve, adding deionized water with 10 times of mass, and extracting for 2 hours at 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 62.5mL, centrifuging to remove precipitate, and obtaining herba cistanches extract.
The preparation method of the yam extract comprises the following steps: weighing 50g of Chinese yam, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 5 times that of the powder, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 137.5mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
The preparation method of the rehmannia root extract comprises the following steps: weighing 50g of rehmannia root, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 10 times that of the rehmannia root, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding 10 times of deionized water for the second time, and extracting at 40 ℃ for 2 hours by ultrasonic treatment; mixing the two extractive solutions, concentrating to 212.5mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
Example 3
The cistanche deserticola used in the implementation is cistanche deserticola, and the origin is the Arena alliance of the inner Mongolia autonomous region; the yam is iron stick yam, the cultivation soil is a soil, and the production area is a city, a county and a temperature county of the Coke of Henan province; the rehmannia root is produced in Henan province and Jiaozhen city and Shang-Feng county.
Extract of traditional Chinese medicine: mixing herba cistanches extract 300 parts, rhizoma dioscoreae extract 300 parts and rehmannia glutinosa extract 25 parts by volume. 2mg of vitamin C is added into each 50mL of the traditional Chinese medicine composition, and the traditional Chinese medicine composition is obtained after even mixing.
The preparation method of cistanche extract comprises the following steps: weighing 50g of cistanche deserticola, crushing, sieving with a 150-mesh sieve, adding deionized water with 10 times of mass, and extracting for 2 hours at 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 75mL, centrifuging to remove precipitate, and obtaining herba cistanches extract.
The preparation method of the yam extract comprises the following steps: weighing 50g of Chinese yam, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 5 times that of the powder, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding deionized water with the mass of 5 times for the second time, and extracting for 2 hours at the ultrasonic 40 ℃; mixing the two extractive solutions, concentrating to 150mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
The preparation method of the rehmannia root extract comprises the following steps: weighing 50g of rehmannia root, crushing, sieving with a 150-mesh sieve, adding deionized water with the mass being 10 times that of the rehmannia root, and extracting for 2 hours at the temperature of 40 ℃ by ultrasonic treatment; extracting twice, adding 10 times of deionized water for the second time, and extracting at 40 ℃ for 2 hours by ultrasonic treatment; mixing the two extractive solutions, concentrating to 225mL, centrifuging to remove precipitate, and obtaining rhizoma Dioscoreae extract.
Example 4
The embodiment provides an application test of a traditional Chinese medicine composition for relieving liver and kidney injury in relieving alcohol-induced visceral injury.
Male SD rats were obtained, and after 1 week of adaptive feeding, they were randomly divided into 5 groups, namely, a blank control group, a model control group, a single dose group, a traditional Chinese medicine extract group, and a test group, wherein the blank control group, the model control group, and the traditional Chinese medicine extract group were 7, and the single dose group was 28, and the test group was 21. The single dose group, the traditional Chinese medicine extract group and the test group are respectively and gastrically administrated with corresponding concentrations and corresponding medicines, and specific groups are shown in table 1, and the blank control group and the model group are gastrically irrigated with equal volume of distilled water for 30 days. After the completion of the stomach irrigation, the rats of each group were irrigated with alcohol (volume fraction: 50%) at a dose of 7g/kg except for a blank control group to which an equal volume of physiological saline was administered; after 16h, the rats were anesthetized with diethyl ether, the femoral artery was sampled, centrifuged at 3000r/min for 10min at 4 ℃, the supernatant was aspirated for storage, and the livers and kidneys were separated in time for weighing storage, and each index was measured.
TABLE 1 test grouping
Note that: the% of the stomach-filling medicine is volume fraction, and the diluent is distilled water.
After alcohol exposure, the rats in the model control group mostly show slow reaction, somnolence, reduced activity, listlessness, face wiping and the like, and some rats initially show overactivity and mania and then turn into a quiet and somnolence state, and individual rats show twitch. Compared with the model control group, the rats with the vitamin C in the stomach removed in the traditional Chinese medicine extract group and the single dose group have better abnormal behavior, but mostly show listlessness and somnolence; compared with the model control group, the mental state and the abnormal behavior of the rats in the traditional Chinese medicine composition group for stomach irrigation are further improved.
The body weight change, liver coefficient and kidney coefficient of the rats were measured, the body weight change was shown in fig. 1, the liver coefficient change was shown in fig. 2, and the kidney coefficient change was shown in fig. 3. As can be seen from FIG. 1, the initial body weight and the final body weight of each group of rats have no obvious difference (P > 0.05), which indicates that rats can not influence the body weight of the rats when the rats ingest the traditional Chinese medicine composition provided by the invention. As can be seen from fig. 2 and 3, compared with the blank control group, the liver coefficient and kidney coefficient of the model control group, the single dose group, the traditional Chinese medicine extract group and the test group are obviously increased (P < 0.05), but the liver coefficient and kidney coefficient of the test group are increased to a lower extent than those of the single dose group and the traditional Chinese medicine extract group (P < 0.05), and the liver coefficient and kidney coefficient of the single dose group and the traditional Chinese medicine extract group are increased to a lower extent than those of the model control group (P < 0.05), which indicates that the liver and kidney toxicity caused by the gastric lavage of alcohol can be effectively reduced by the ingestion of the traditional Chinese medicine composition provided by the invention by rats, and the application effect is better than that of the combination of each single dose and the traditional Chinese medicine extract.
Rat serum alanine aminotransferase (alanine aminotransferase, ALT) activity, aspartate aminotransferase (aspartate aminotransferase, AST) activity, glucose (GLU) concentration, urea nitrogen (blood urea nitrogen, BUN) concentration and Creatinine (CREA) concentration were determined using an AU400 fully automated biochemical analyzer (new technology, inc. Of the family of the mansion, inc.). The reagents used for measuring the index in this example were all from the new technology Co., ltd. ALT activity changes and AST activity changes are shown in FIG. 4, GLU concentration changes and BUN concentration changes are shown in FIG. 5, and CREA concentration changes are shown in FIG. 6.
The elevation of ALT and AST activity is considered to be the most sensitive indicator of impaired liver function. As shown in fig. 4, the results of this example show that, after the alcohol lavage and the infection, the serum ALT and AST activities of the rats in the model group are significantly increased (P < 0.05) compared with the blank control group, and the results indicate that the preparation of the acute alcoholic injury model of the rats is successful. The results show that the disposable high-dose alcohol contamination can cause the rise of serum transaminase and cause acute liver function injury. The test result also shows that the traditional Chinese medicine composition provided by the invention can effectively reduce acute liver function injury caused by alcohol gastric lavage, and the application effect is better than that of the combination of each single dose and the traditional Chinese medicine extract (P is less than 0.05).
The concentration of GLU in serum is regulated by a number of factors, of which the kidneys also have a regulatory function, mainly by reabsorption of the tubular and collecting ducts, while BUN and CREA in serum are important indicators for clinical evaluation of renal function, indicating that renal function may be abnormal when the concentration of GLU, BUN and CREA in serum is elevated. As shown in fig. 5 and 6, the concentrations of GLU, BUN and CREA were significantly higher in the model control group than in the normal control group, suggesting that alcohol may cause damage to renal function in the rats, such as the reabsorption ability of the renal tubules and glomerular filtration function. Compared with a model group, the concentrations of GLU, BUN and CREA of rats in a single dose group, a traditional Chinese medicine extract group and a test group are reduced (P is less than 0.05), and the traditional Chinese medicine composition provided by the invention can effectively reduce acute kidney function injury caused by alcohol gastric lavage, and the application effect is better than that of the combination of each single dose and the traditional Chinese medicine extract (P is less than 0.05), so that the traditional Chinese medicine composition provided by the invention has a certain synergistic effect among the components.
Example 5
The embodiment provides an application test of a traditional Chinese medicine composition for relieving liver and kidney injury in relieving fructose-induced visceral injury.
Male SD rats were obtained, and after 1 week of adaptive feeding, they were randomly divided into 5 groups, namely, a blank control group, a model control group, a single dose group, a traditional Chinese medicine extract group, and a test group, wherein the blank control group, the model control group, and the traditional Chinese medicine extract group were 7, and the single dose group was 28, and the test group was 21. The single dose group, the Chinese medicine extract group and the test group were respectively administered with corresponding concentrations and corresponding medicines by gavage, and the specific group is as shown in table 1 in example 4, and the blank control group and the model group were gavaged with equal volume of distilled water. Rats were dosed daily with 10% fructose solution for molding, except for normal drinking water given to the placebo group. At the end of the intragastric period on day 40, rats were each transferred to a metabolic cage and then sacrificed after fasting without water on the last day.
The rat liver tissue was accurately weighed after washing with physiological saline, and was homogenized with physiological saline to prepare 10% liver, centrifuged at 4000rpm at 4℃for 10min, and the levels of Xanthine Oxidase (XO) and adenosine deaminase (Adenosine deaminase, ADA) in the liver tissue were measured according to the kit instructions. The kidney tissue of rat was accurately weighed after washing with physiological saline, and was homogenized with physiological saline to prepare 10% liver, centrifuged at 4000rpm at 4℃for 10min, and the levels of Malondialdehyde (MDA), superoxide dismutase (Superoxide dismutase, SOD) and Catalase (CAT) in the kidney tissue were measured according to the kit instructions. The kits used in this example were all from the institute of bioengineering, built in Nanjing. The XO level change and ADA level change are shown in fig. 7, the MDA concentration change is shown in fig. 8, the SOD concentration change is shown in fig. 9, and the CAT concentration change is shown in fig. 10.
Uric acid synthesis in vivo is mainly performed in the liver, xanthine oxidase and adenylate deaminase are key enzymes responsible for uric acid synthesis in purine metabolism, uric acid level in serum can be remarkably reduced only by inhibiting XO activity, and diseases such as hyperuricemia and the like can be effectively improved. Thus, the enzymatic activity of XO and ADA can have a significant impact on serum uric acid levels. As shown in fig. 7, the single dose group, the chinese herb extract group, and the test group reduced serum uric acid levels by reducing expression of enzymes of XO and ADA by decreasing uric acid synthesis, as compared to the model control group. Moreover, rats can effectively reduce liver function damage caused by fructose after taking the traditional Chinese medicine composition provided by the invention, and the application effect is superior to that of the combination of each single dose and the traditional Chinese medicine extract (P is less than 0.05), which shows that the traditional Chinese medicine composition provided by the invention has a certain synergistic effect among the components.
Hyperuricemia develops due to high fructose diets promoting the synthesis of uric acid in large amounts in the body. In the way of synthesizing uric acid in vivo, a large amount of superoxide anions are generated to cause the formation of free radicals, and the excessive generation of the superoxide anions can destroy the normal physiological order in vivo, so that lipid peroxidation, protein oxidative denaturation and DNA molecular crosslinking of biological membranes of organisms are caused, and oxidative stress damage occurs. MDA is an intermediate product of lipid peroxidation and can severely disrupt cellular components, structure and function in amounts that can reflect the extent of lipid peroxidation in vivo. The in vivo antioxidant enzyme system comprises SOD and CAT, and the level of the SOD and CAT can well measure the oxidative stress injury degree of viscera. As shown in fig. 8-10, MDA activity was significantly increased in the model control group, single dose group, traditional Chinese medicine extract group, test group, and SOD and CAT activity were significantly decreased (P < 0.05) compared to the blank control group; MDA activity was significantly increased in the single dose group, the traditional Chinese medicine extract group, the test group, and SOD and CAT activity were significantly reduced (P < 0.05) compared to the model control group; MDA activity in the traditional Chinese medicine extract group, the test group is significantly increased, SOD and CAT activity is significantly reduced (P < 0.05) compared with the single dose group; compared with the traditional Chinese medicine extract group, the MDA activity in the test group is obviously increased, and the SOD and CAT activities are obviously reduced (P < 0.05). The result shows that the traditional Chinese medicine composition provided by the invention can obviously improve the antioxidation capability in the kidney, reduce lipid peroxidation in vivo, protect rat kidney injury caused by high fructose diet, and has a certain synergistic effect among the components.
The present invention may be better implemented as described above, and the above examples are merely illustrative of preferred embodiments of the present invention and not intended to limit the scope of the present invention, and various changes and modifications made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the present invention without departing from the spirit of the design of the present invention.

Claims (1)

1. The traditional Chinese medicine composition for relieving liver and kidney injury is characterized by comprising a traditional Chinese medicine extract and vitamin C;
the traditional Chinese medicine extract consists of the following components in parts by volume: 100-300 parts of cistanche extract, 100-300 parts of yam extract and 12-25 parts of rehmannia root extract; the cistanche extract, the yam extract and the rehmannia root extract are all water extracts;
the ratio of the traditional Chinese medicine extract to the vitamin C is 10-50:1-2 in terms of mL in mg;
the preparation method of the cistanche extract or the yam extract or the rehmannia root extract comprises the following steps:
pulverizing cistanche, yam or rehmannia root, sieving, extracting with water for 2-4 times under the ultrasonic condition of 35-40 ℃ for 1-2 hours each time, and combining the extracting solutions of each time;
the step of concentrating and removing sediment is also carried out before the extracting solutions are combined; in the preparation method of the cistanche extract, the concentration is that the ratio of the concentrated solution of the combined extract to the cistanche is (1-1.5) 1 in terms of mL g;
in the preparation method of the yam extract, the concentration is that the ratio of the concentrated extract liquid after combination to the yam is (2.5-3) 1 in terms of mL g;
in the preparation method of the rehmannia root extract, the concentration is that the ratio of the concentrated extract liquid after combination to the rehmannia root is (4-4.5) 1 in terms of mL.
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106074984A (en) * 2016-08-26 2016-11-09 北京致成生物医学科技有限公司 A kind of the root bark of persimmon tree medicine drink and its preparation method and application

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106074984A (en) * 2016-08-26 2016-11-09 北京致成生物医学科技有限公司 A kind of the root bark of persimmon tree medicine drink and its preparation method and application

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
大鼠运动性肾脏缺血再灌注损伤及肉苁蓉配伍维生素C的保护作用;周俊飞等;《山东体育学院学报》;20140430;第30卷(第2期);70-74 *

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