CN102526346B - Health-care pharmaceutical formulation with immunity-enhancing and senility-delaying functions - Google Patents

Abstract

A health-care pharmaceutical formulation with immunity-enhancing and senility-delaying functions relates to health-care pharmaceuticals and comprises broccoli extractive 9-20g, taurine 0.4-1g, vitamin C 0.4-1g, vitamin E 0.4-1g, Chinese wolfberry fruit 9-15g, ginseng 4.5-9g, ginkgo leaf powder 9-12g, tea polyphenol 2.2-4g, microcrystalline cellulose 3.6-9g, dextrin 3.1-8g, pregelatinized starch 2.5-6g, powdered sugar 2.5-8g, povidone 0.4-1g and magnesium stearate 0.3-1g according to formulation proportion. A preparation process comprises the steps: smashing Chinese wolfberry fruit and ginseng pharmaceuticals respectively, using a sieve with 100-120 meshes to perform screening, respectively weighing raw materials and auxiliary materials according to a certain proportion to be mixed for 45-50 minutes and then performing tabletting and controlling tablet weight to be in the inner quality standard range; performing next working procedures including screening, polishing, inner packing and outer packing and finally performing inspection and warehousing according to the inner quality standard. The health-care pharmaceutical formulation with the immunity-enhancing and senility-delaying functions can enhance immunity of users, delay senility and improve human body immunity and life quality of the users, and has no side effect and untoward effect. By using a modern preparation technology and an advanced quality control method, products are safe, stable and controllable in quality.

Description

The health medicine of a kind of enhancing immunity, deferring senility

Technical field:

The present invention relates to the formula of health-care medicine of a kind of enhancing immunity, deferring senility.

Background technology:

This product is suitable for the extensive crowd of hypoimmunity.According to one of World Health Organization (WHO), global the survey showed that, and the people of whole world true health only accounts for 5%, through doctor, checks, diagnoses ill people also only to account for 20%, 75% people in sub-health state.In May, 2002, the crowd of CPMA inference China in sub-health state is 70% (approximately 800,000,000), and most people's hypoimmunity." in healthy energy deposits, heresy can not be done, and the institute of heresy gathers, and its gas must be empty ".Mean: while there is very strong resistance in human body, disease is impossible invade health, disease place of arrival, its resistance is certainly weak.The basic reason that people falls ill is that resistance (medically claiming immunity) is low.Modern medicine study proves, eliminates subhealth state, prevent disease, and the most effective way is exactly to strengthen the immunity of human body to disease.Therefore develop effect uniqueness, have no side effect, the health product with enhancing immunity function of high in technological content, pure natural, necessarily there is wide market prospect and social benefit preferably.China has entered aging society, and aging process is quite rapid, for ensureing the aged's who sharply increases health. to alleviate family and burden on society, the research of slow down aging has become current very urgent task.Modern medicine is about the research of old and feeble cause and mechanism, there is no at present final conclusion, though conventional defying age method has certain effect, but the relative unicity of mechanism of action is difficult to adapt to the old and feeble feature with whole body multisystem, multilevel change, in contrast to this, Traditional Chinese Medicine medication have effect comprehensively, less toxic side effect and other advantages.This problem is observed from clinical practice, the patient who finds nephrasthenia syndrome is no doubt many, but deficiency of both QI and YIN has also accounted for significant proportion, and usually with the sign of blood stasis, therefore propose thinking and the method for benefiting qi and nourishing yin activating blood circulation method slow down aging, and from the aspect such as theory, Clinical and experimental study, inquire into theoretical basis, clinical effectiveness and the mechanism of action of benefiting qi and nourishing yin activating blood circulation method, Ji provides new approach for slow down aging.In theoretical research, according to successive dynasties document to old and feeble understanding, simultaneously in conjunction with researcheres both at home and abroad about old and feeble research report and tutor's experiences in diagnosis and treatment, think traditional internal organs void not yet essence of complete reaction aging of idea that declines, deficiency of both qi and yin with blood stasis is also the main aspect of aging.Old and feeble commonly encountered diseases is because having: congenital defect, and vigour is lost and is filled; Eating and drinking without temperance, spleen fortune is lost strong; Feelings will is unsuccessful, stagnated fire impairment of YIN; Work and rest is uncomfortable, consumption impairment of YIN blood; Self-care is inconsiderate, interior raw blood stasis; Exopathogen invasion and attack, wound is just consuming the moon.These factors all can have influence on the mechanism of qi of body, cloudy liquid and blood situation, thereby cause deficiency of both qi and yin with blood stasis.Main pathogenesis changes to be had: deficiency of vital QI, involves the five internal organs; Deficiency of YIN fire is raw, and bones of the body collectively loses supports; Stagnation of blood stasis, channels is obstructed; Vital QI being weakened and pathogen being violent is mutually mixed.In old and feeble pattern of syndrome, the situation of the simple deficiency of vital energy or the deficiency of YIN and blood stasis is actually rare, but the folder of often mutually holding concurrently is for disease, reciprocal causation.Deficiency of both QI and YIN can cause the deficiency that essence, blood, body fluid generate, so that the five internal organs lose, supports and old and feeble, and deficiency of both QI and YIN can cause the pathologic products such as blood stasis simultaneously, aggravation senescence process; The deficiency of YIN is the important pathologic basis of blood stasis, after blood stasis forms, can consume cloudy liquid again, the aggravation deficiency of YIN.Blood stasis can cause internal organs malfunction, and the function of the raw cloudy liquid of causing is affected, so that cloudy liquid further loses; It is difficult multiple and hemorrhage that blood stasis easily causes network to damage, and hemorrhagely makes cloudy blood more consume wound; Blood stasis also can cause ascending or descending movement of vital Qi not normal, and it is smooth that gas loses a surname, and body fluid is compress normally, and cause Tianjin impairment of YIN, loses more very.Therefore, the deficiency of YIN can cause the stasis of blood, and the formation of blood stasis can increase the weight of the deficiency of YIN again, is also interactional between the two.Moreover deficiency of both QI and YIN can cause the generation of multiple Senile disease, and after disease occurs, can damage again Old Men, further cause human righteousness not enough, hypofunction, accelerates old and feeblely, and forms vicious cycle.In old and feeble process, exist the pathomechanisms such as the cloudy fEl that the deficiency of vital energy, the deficiency of YIN and blood stasis etc. cause is unbalance, internal organs lose and support, blockage of meridian, also exist changing in pathological products such as endogenous fire, blood stasis etc., so, regulating YIN and YANG, the virtual loss of supplementing nutrition, promoting blood circulation to remove obstruction in the collateral, the clear remaining heresy of holding concurrently should be the main aspects for the treatment of.Main method for the treatment of has: QI invigorating invigorating middle warmer, reinforcing body resistance; YIN nourishing fluid infusion, guards against taste greasy; Blood circulation promoting and blood stasis dispelling, is cautious use of and sends an expedition against; Rush down clearly pathogenic heat, protect cloudy relieving restlessness.During Selection drug, mainly take sweet temperature QI invigorating, sweet cold YIN nourishing, tonify deficiency righting, the eliminating evil reality of holding concurrently is principle, takes into account the medication plus-minus of less important disease simultaneously, and invigorating middle warmer is held concurrently and dredged, reside eliminating evil among righting, and be the method for giving consideration to both the incidental and fundamental.

According to market survey, although it is many to have in the market the health product of identical function, market demand is still huge, the Caulis et Folium Brassicae capitatae nutritious, effect is unique of in addition take be primary raw material exploitation product but seldom, so market has to be developed.This product has following characteristics: this formula for a product rationally, definite ingredients, effect be reliable; Take safe, without any side effects; Easy to carry, be convenient to preserve; Process technology is advanced, quality guaranteed (this product production and processing overall process is carried out in strict accordance with GMP standard).Caulis et Folium Brassicae capitatae in formula is the sharpest edges of this product; the contained nutrient substance of Caulis et Folium Brassicae capitatae is very abundant; not only containing protein, sugar, fat, mineral, vitamin C and carotene etc.; glucosinolate content is very abundant especially; take glucorphanin (Glucoraphanin) as main; its catabolite sulforaphen is the strongest active component of anticancer vigor of finding in vegetable up to now; the have clear improvement microenvironment of human body cell of tool; can improve body immunity, promote the effect of death of neoplastic cells.Therefore compared with other products, the health-care effect of this product is even better.

China is engaged in research and the production of natural drug, there is advantageous condition, the rarity of Traditional Chinese Medicine, it has the clinical experience of several thousand, from current pharmaceutical research trend, from natural product, find new drug or find that guide is combined to thing and it is carried out to the important content that structural modification is drug development, along with the people that day by day seriously make of social environmental pollution more and more like pure natural, pollution-free, the food that do not add synthetics.Adding growth in the living standard also makes people have higher pursuit to the quality of life.Under this background, this formula uses the antioxidant functional food that high-tech means the free of contamination pure natural animals and plants of take are raw material to arise at the historic moment, and develop rapidly.

Summary of the invention:

The formula of health-care medicine that the object of this invention is to provide a kind of enhancing immunity, deferring senility, its spy, it can strengthen people's immunity, slow down aging, improve people's living standard, have no side effect and untoward reaction, and use modern preparation technique, advanced method of quality control, makes product accomplish safety, stable, quality controllable.

In order to solve the existing problem of background technology, the present invention is by the following technical solutions:

The health medicine of a kind of enhancing immunity, anti-senescence function; it is characterized in that; comprise Caulis et Folium Brassicae capitatae, taurine, vitamin C, vitamin E, Fructus Lycii, Radix Ginseng, Folium Ginkgo extract, tea polyphenols, microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate, each component ratio is: Caulis et Folium Brassicae capitatae 20g, taurine 1g, vitamin C 1g, vitamin e1 g, Fructus Lycii 15g, Radix Ginseng 9g, Folium Ginkgo extract 12g, tea polyphenols 4g, microcrystalline Cellulose 9g, dextrin 8g, pregelatinized Starch 6g, Icing Sugar 8g, polyvidone 1g, magnesium stearate 1g.

Its preparation technology is: first Fructus Lycii, Radix Ginseng medicine pulverized respectively, crossed 100-120 mesh sieve, get respectively supplementary material and weigh by a certain percentage, carry out supplementary material mixing, and incorporation time 45-50 minute, then carry out tabletting, within control strip focuses on inner quality standard.Carry out next procedure screening, polishing, inner packing, outer package, finally by inner quality standard check warehouse-in.

After above-mentioned processing technique, in medicine of the present invention, preferably active constituent content distributes as follows: 100g is containing the sweet 730mg of effective ingredient Radix Raphani sulfur, polysaccharide 3700mg, total saponins 1800mg.

The present invention finds through test, the medicament being mixed according to the above ratio has the effect of the enhancing body immunity slow down aging better than simple one or both compositions, and is overcome through the adverse effect that the proportioning of above-mentioned various composition specific proportions has beat all health-care effect and former each composition.

Especially more preferably, during this production, supplementary material mixing time 45-50 minute is row tabletting again, the results showed, the long or too short drug effect for last preparation of incorporation time of supplementary material has adverse effect.45-50 minute for guaranteeing that final drug effect has important function.

The present invention has following beneficial effect: immunity, the slow down aging that can strengthen people, the health level that improves people, has no side effect and untoward reaction, and uses modern preparation technique, advanced method of quality control, makes product accomplish safety, stable, quality controllable.

The specific embodiment:

Caulis et Folium Brassicae capitatae:

Caulis et Folium Brassicae capitatae raw material in formula is made through alcohol extraction process with our common vegetable Caulis et Folium Brassicae capitatae used in everyday.Caulis et Folium Brassicae capitatae meets < < Ministry of Public Health about the notice > > requirement of further standard healthy food material management as bread and cheese raw material, the nutrient substance that Caulis et Folium Brassicae capitatae is rich in it and the unique effects having, along with progressively going deep into of scientific research, more and more be subject to people's attention, and start to have obtained exploitation faster and application at field of food.Caulis et Folium Brassicae capitatae is not only containing protein, sugar, fat, mineral, vitamin C and carotene etc.; glucosinolate content is very abundant especially; take glucorphanin (Glucoraphanin) as main; its catabolite sulforaphen is the strongest active component of anticancer vigor of finding in vegetable up to now; the have clear improvement microenvironment of human body cell of tool; can improve body immunity, promote the effect of death of neoplastic cells.

External related documents has reported that Caulis et Folium Brassicae capitatae has enhancing immunity function, P.Thejass for example, in " probe into the immunocompetence of sulforaphen, a kind of from Brassica oleracea L. var. botrytis L. the isothiocyanate of natural extract " literary composition that G.Kuttan delivers on < < plant amedica (magazine) > >, experimental result shows: sulforaphen is improved the effect of the peritoneal macrophage of activate the phagocytic capacity.In addition the effect of sulforaphen also can greatly reduce the tumor necrosis factor increasing---and a lipopolysaccharide stimulates the macrophage producing.These results show, sulforaphen has the immunocompetent effect of adjusting.; According to Thejass P, " a kind of natural isothiocyanate that derives from Caulis et Folium Brassicae capitatae of sulforaphen increases the cytotoxicity of BALB/c mouse NK cell and antibody dependence by strengthening interleukin II and tumor necrosis factor α " literary composition report that Kuttan G delivers on < < ImmunopharmacologicaResearch and immune toxicity magazine > >: the research of sulforaphen on the cell-mediated immune impact of the BALB/c mouse of normal and Ehrlich ascites inoculated tumour.Sulforaphen significantly increases the NK cytoactive of normal and inoculated tumour animal.NK cytoactive in intact animal is than inoculated tumour cell animal Zao being observed more.The cytotoxicity that the antibody of the animal of the normal and inoculated tumour that sulforaphen is processed relies on is significantly strengthened than the animal of the inoculated tumour that does not have sulforaphen to process.In the animal of the normal and inoculated tumour of processing at sulforaphen, observe a kind of cytotoxicity of complementation mediation of early antibody dependence.Sulforaphen is processed interleukin II and tumor necrosis factor-gamma in the animal that has significantly increased normal and inoculated tumour.Sulforaphen is processed and has significantly been promoted splenocyte, medullary cell, thymocyte proliferation in addition.Show that sulforaphen has the effect that strengthens immunologic function; According to Thejass P, Kuttan G on < < ImmunopharmacologicaResearch and immune toxicity magazine > >, deliver " sulforaphen is to B16F-10 melanomainduction migrationproperty tumor inoculation the adjusting of response of C57BL/6 mouse cell mediated immunity " a literary composition report: sulforaphen is processed and has significantly been strengthened migrationthe NK cytoactive of property tumor inoculation animal, has significantly increased inoculation melanomaanimal in interleukin II and tumor necrosis factor α, sulforaphen has significantly reduced the level of proinflammatory Fine intracellular cytokine toxicity in the serum during neoplasm metastasis in addition, by the response of sulforaphen irritation cell mediated immunity, raise IL-2 and IFN-gamma, lower proinflammatory Fine intracellular cytokine toxicity IL-1beta, IL-6, TNF-alpha and GM-CSF.。

Domestic related documents has reported that Caulis et Folium Brassicae capitatae has enhancing immunity function, Ji Yubin for example, Chi Wenjie, Zou Xiang, in the < < Caulis et Folium Brassicae capitatae that Wang Zhiju delivers on the journal > > of < < Harbin University of Commerce (proceeding to science version), sulforaphen extracts, in separation and Anticancer Activities > > mono-literary composition, show that sulforaphen is to rodent hepatocarcinoma, breast carcinoma, pulmonary carcinoma, the esophageal carcinoma, front gastric cancer has obvious blocking effect especially to hepatocarcinoma, the esophageal carcinoma, front Effect on Gastric Cancer is obvious, experimental results show that the generation that they can Phase II (cancerigenic factor removing toxic substances) enzyme, thereby play antitumaous effect, these enzymes can be by destroying the active center of cancerigenic factor or the method that they and endogenous aglucon are combined, acceleration is excreted.Data proves, sulforaphen is by carcinogen metabolic regulation mechanism is worked.The research worker of U.S. Han Si John Hopkins University utilizes transgenic mouse experimental results show that: a kind of system of ubiquity in many animal bodies, the carcinogenesis that is used for resisting toxic chemical substance in food and environment.The performance of this effect, depends on the remarkable increase of Phase II enzyme quantity.This enzyme can be eliminated harmful chemical, suppresses its infringement DNA, thereby reaches anticancer effect.Two sulforaphen are the strongest Phase II enzyme inducers of finding up to now, according to the Zhou Jinlan of department of chemistry of the Central China University of Science and Technology, in the purification of Yu Kaichao main sulfur glycosides in < < applied chemistry > > (the 22nd the 10th phase of volume) delivers < < Semen Brassicae campestris and anti-tumor activity > > mono-literary composition, show: sulfur glycosides itself is kind of a more stable compound, but can be become isothiocyanate (ITC) by bacterial enzyme catalytic degradation in myrosase or gastrointestinal tract, the multi-products such as oxazolidine thione (OZT) and nitrile compounds.These catabolites have the antibacterial action of stronger Antifungi and microbial reproduction etc. and by induction ubiquinone reductase activity, block the carcinogenesis of carcinogen, according to the first middle school, Qinhuangdao, Hebei province, Hebei Normal University's chemistry and material science institute Shen tree virtue, Zhang Yingfeng, nightstool river was < < chemistry teaching > > (2009, the 7th phase) in the < < Caulis et Folium Brassicae capitatae of delivering on, in Anticancer > > mono-literary composition of thioglycoside, show that glucorphanin can induce body to produce II type detoxication enzyme-glutathione transferring enzyme and quinone reductase, this enzyme can produce resistance to many carcinogens, thereby play antitumaous effect, according to Beijing University of Technology's engineering constraint, learn, The Hong Kong Polytechnic University's applying biological and chemical science and technology are Wang Jiandong, Yuan Qipeng, it is as follows that the < < sulforaphen progress > > mono-that Qian Zhongming delivers at < < food and fermentation industries > > the article pointed out that sulforaphen acts on body process: the transcribing of (1) enzyme II gene.Transcribe and only produce the II type enzyme useful to human body, inhibitory enzyme I produces simultaneously, and enzyme I can produce cooperative effect with carcinogen, accelerates cancer and occurs.(2) enzyme II acts on the anticancer system of body self.By this system, can be achieved as follows regulation and control to metabolism: 1. block carcinogen and produce the required metabolic pathway of carcinogenic effect, stop carcinogen or its metabolite to enter target cell, play the carcinogenic effect of removing simultaneously; The division of anticancer and growth, blocking-up circulation approach; Promote the effect of the red carcinogen induction of other protein secreting ancestral that can kill cancerous cell cancer.2. the startup generation effect that the hydrolyzate of sinigrin may play a role to carcinogen, at the formation of cancer initial stage, gene is undergone mutation, also have in addition some inhibitory enzymes to there is dual resistance cancer or cancer suppressing function, Toxic is degraded, thereby play anticancer effect-become and press down cancer approach, according to Zhejiang Prov Industrial And Commercial University's food, be Wang Zhongying, in the < < thioglycoside that Wang Xiangyang delivers at the chemical > > (25 4 phases of volume in 2005) of < < life and catabolite isothiocyanate > > mono-literary composition thereof, show: the anticancer mechanism of isothiocyanate comprises two parts: (1) stops (comprising cytochrome p450 system) process of chemocarcinogenesis by suppressing Phase I enzyme, particularly PEITC and BITC can effectively suppress cancerigenic factor-DNA and add the formation with thing, thereby forming of the tumor that inhibition is induced by cancerigenic factor, (2) induction produces Phase III detoxication enzyme, such as glutathione transferase and UDPG aldehydic acid based transferase etc., thereby reduce the overactive carcinogenic intermediate product that cytochrome p450 system produces.Wherein to produce the ability of Phase III detoxication enzyme strong especially for SUL induction, is the natural inducer of the most effective Phase III detoxication enzyme that identifies up to now.Although the SUL of high concentration and fragrant alkane isothiocyanate have certain toxic action to human body, they,, in valid density (0.05～20umol), do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent kinds cancer.

With the Caulis et Folium Brassicae capitatae consumption in this formula, according to hiding naval, in the < < dregs of rapeseed cake that Zhang Keying delivers at < < feed industry > > (the 28th volume in 2007), in the harm of thioglycoside and detoxification Study on Measures > > mono-literary composition, show: pig, rabbit, fowl, fish and ruminant are respectively 0.78 to the tolerance level of thioglycoside in daily ration, 7.0, 5.4, 3.6 and 1.5～4.22umol/g, in < < applied chemistry > > (the 20th the 10th phase of volume) < < Semen Brassicae campestris, in the purification of main sulfur glycosides and anti-tumor activity > > mono-literary composition, show, mice sulfur glycosides least action dosage 30mg/kg, shows that sulfur glycosides has obvious inhibitory action to S180 tumor in Mice Body, in the < < thioglycoside that the chemical > > of < < life (25 4 phases of volume in 2005) delivers and catabolite isothiocyanate > > mono-literary composition thereof, show: although the SUL of high concentration and fragrant alkane isothiocyanate to human body have certain toxic action they in valid density (0.05～20umol), do not show 200～400 milligrams of the oral suggestions of toxicity human body, can effectively prevent kinds cancer.According to above different documents and materials, the glucorphanin in this formula Caulis et Folium Brassicae capitatae raw material of take is test raw material, by adult (50kg weighing machine) 34mg every day, tests.

1. 30 times, 10 times of human body recommended amounts are established in ear swelling method experiment, according to formula, calculate: every day, glucorphanin 22.48mg/kg, 8.16mg/kg were respectively high low dose group.During actual disposition gavage liquid, need the high low dose group 27.98mg/kg of sample glucorphanin, 9.33mg/kg, dosage 0.4ml/20g Mus weight/d.Every day gastric infusion, experimental result in Table 1, table 2.

Table 1 glucorphanin immunological enhancement: body weight organ index

Table 2 sulforaphen glucorphanin immunological enhancement: ear swelling

Experimental result, compares the high low dosage of glucorphanin body weight is not had a significant effect with blank group, but ear swelling experiment shows, high low dose group all has obvious immunological enhancement.

2. humoral immunization serum hemolysin experiment, the high 1.2mg/ml of glucorphanin; The low 0.4mg/ml of glucorphanin, dosage 0.4ml/20g/d, every day gastric infusion.The results are shown in Table 3 tables 4:

Table 3 sulforaphen glucorphanin immunological enhancement: body weight organ index

Table 4 sulforaphen glucorphanin immunological enhancement: serum hemolysin

Experimental result, compares with blank group, and positive drug Thymosin alpha 1 is influential to body weight, and the determination experiment of serum hemolysin shows its obviously enhancing body humoral immunization.All the other each group and normal group comparisons, to body weight and organ index all without remarkable effect.The serum hemolysin of the high low dosage of glucorphanin and antibody product are improved, p < 0.05, and low metering group also improves with respect to blank group.The determination experiment of serum hemolysin shows, just metering group of glucorphanin has certain enhancing immunity effect.

Taurine

Taurine (Taurine) chemistry 2 one taurine sulfur-containing amino acid by name.Recent study shows; taurine can promote the metabolism such as fat, protein and mineral element; there is the functions such as hepatic cholagogic, anti-hypercholesterolemia and atherosclerosis; and as antioxidant and membrane stabilizer protection body cell, and nerve, endocrine, reproduction and immune system etc. are had to regulating action.

1, the impact of taurine on MDA content, the active GSH of SOD and lipofuscin content in motion rat heart muscle

By table 5, known, power exhausts motion and can cause MDA content and lipofuscin content in rat heart muscle tissue significantly to raise, and the active and GSH content of SOD significantly declines; And MDA and lipofuscin content significantly reduce after exhausting motion compared with power in the visible rat heart muscle tissue of supplementation of taurine, after exhausting motion compared with power, the active and GSH content of SOD significantly improves.Explanation of force exhausts motion and can cause rat heart muscle to organize level of lipid and lipofuscin content to significantly improve, and taurine can antagonism power exhausts the raising of level of lipid and lipofuscin content in motion rear myocardium tissue.

The impact of table 5 taurine on MDA content, SOD activity, GSH and lipofuscin content in motion rat heart muscle tissue

Note: * is for to compare with A group P < 0.01; △ is for to compare with B group, P < 0.01

2, (by table 6, known, power exhausts motion and can cause MDA and lipofuscin content in rat brain tissue significantly to raise, and SOD activity and GSH content significantly decline in the impact of taurine, GSH active on MDA content in motion rat brain, SOD and lipofuscin content; And MDA and lipofuscin content significantly reduce after exhausting motion compared with power in the visible rat brain tissue of supplementation of taurine; active and the GSH content of SOD exhausts and significantly improves explanation of force after motion and exhaust motion and can cause rat brain to organize level of lipid and lipofuscin content to significantly improve compared with power, and taurine can antagonism power exhausts the motion raising of level of lipid and lipofuscin content in cerebral tissue afterwards.

The impact of table 6 taurine on MDA content, SOD activity, GSH and lipofuscin content in motion rat brain tissue

Note: * is for to compare with A group P < 0.01; △ is for to compare with B group, P < 0.01

3, the impact of taurine on DM rat body weight kidney weight, urine amount, blood glucose, serum uric acid level and urinary albumin excretion

DM rat body weight is compared obvious reduction with matched group, kidney/weight ratio obviously raises.Taurine treatment group kidney/weight ratio slightly declines, but difference not statistically significant.Compare with matched group, DM rat blood sugar level obviously raises, taurine treatment group blood sugar level and DM rat indifference.DM rat serum uric acid level and urinary albumin excretion be apparently higher than normal control, and the DM rat serum uric acid level that gives taurine declines, and with normal control indifference, urinary albumin excretion also reduces, but still higher than normal control.

The impact of table 7 taurine on rat body weight/kidney weight, urine amount, blood sugar level and urinary albumin excretion

Note: for comparing with diabetic groups, * P < 0.01; For comparing with normal group, △ P < 0.05, △ △ P < 0.014,

4, the impact of taurine on rat silicosis oxidant and anti-oxidant system index

DM renal cortex of rats SOD and GSH-Px activity are all starkly lower than matched group (P < 0.05), and serum and renal cortex MDA level are apparently higher than matched group (P < 0.05); Give the DM rat SOD of taurine and GSH-Px activity apparently higher than untreated DM rat and matched group (P < 0.05), serum and renal cortex MDA level are starkly lower than DM rat (P < 0.01, P < 0.05), but still higher than matched group, serum MDA level is still higher than matched group (P < 0.01); Renal cortex MDA level is also higher than matched group, difference not statistically significant.

The impact of table 8 taurine on rat silicosis oxidant and anti-oxidant system index

Note: for comparing with diabetic groups, * P < 0.01; For comparing with normal group, △ P < 0.05, △ △ P < 0.014,

5, the impact of taurine on aged male rats NOS, NO, GSH mono-Px and GSH

Taurine and antiperspirant alanine have no significant effect (P > 0.05) to senile rat serum NO S vigor and NO content.Taurine group rat blood serum GSH mono-Px vigor significantly improves (P < 0.05) than matched group, Beta-alanine group; GSH content significantly improves (P < 0.05) than matched group, than Beta-alanine group extremely significantly raise (P < 0.01).Beta-alanine group rat blood serum GSH mono-Px vigor is compared with matched group and is not obviously changed (P > 0.05), and GSH content is than the obvious reduction of matched group (P < 0.05)

The impact of table 9 taurine on aged male rats serum NO level S, NO, GSH mono-Px and GSH level

6, the impact of taurine on aged male rats MDA, SOD, CAT and total antioxidant capacity (T mono-AOC)

Compare with matched group, taurine group rat blood serum MDA content significantly reduces (P < 0.01), T mono-AOC significantly strengthens (P < 0.01), CAT vigor significantly raises (P < 0.05), and SOD vigor does not have significant change (P > 0.05); Beta-alanine group rat blood serum MDA content significantly raise (p < 0.01), T mono-AOC and SOD vigor significantly reduce (P < 0.01), and CAT vigor significantly reduces (P < 0.05).Compare with p mono-alanine group; taurine group rat blood serum MDA content extremely significantly reduces (P < 0.01); T mono-AOC and CAT activity extremely significantly strengthen (P < 0.01), and SOD vigor significantly improves (P < 0.05).

The impact of table 10 taurine on aged male rats serum NO level S, NO, GSH mono-Px and GSH level

NOS is the synthetic key enzyme of NO, and its energy catalyzing levorotatory arginine (L mono-Agr) and molecular oxygen reaction generate NO so also commonly use NOS vigor and indirectly reflect synthesizing of endogenous NO.NO is a kind of free radical, and the excessive generation of NO can cause hypotension shock, cell injury, until multiple pathological lesions such as the nitrous phthalein of nucleus, deaminizating oxidation, destruction DNA structures.The cytotoxicity of NO is also embodied in and can suppresses energy metabolism, disturbs glycolysis, Rhizoma Sparganii acid circulation and oxidative phosphorylation.The generation of numerous disease all with NO extremely relevant in body.The research of Xu Zhengrong etc. shows: taurine has no significant effect river to dying silicious dust lung tissue of rats NOS expression.Feng Ying etc., to adding taurine in all ages and classes male rat drinking-water, find that taurine has no significant effect river to serum NO content.This results show, active and NO content does not have a significant effect to aged male rats NOS for taurine and taurine transporter inhibitor (β mono-alanine), and this shows that taurine may not bring into play antioxidation by NOS mono-NO approach.GSH mono-Px is the reduction reaction of catalytic reduction type GSH to hydroperoxides specifically, it is generally acknowledged that it can remove harmful peroxide metabolite in cell, blocking-up lipid peroxidation chain reflex, thus play the effect of Cell protection membrane structure and telotism.GSH is a kind of low molecule free radical scavenger, can remove LOOH, the substrate of GSH or GPX and two kinds of enzymes of CST, by these two kinds of enzyme hydroperoxide decompositions essential, and can stablize containing the enzyme of coloured glaze base and prevent hemoglobin and the oxidated damage of other cofactors, the important use of play prevention, recovery in the processes such as atherosclerosis, coronary heart disease, anti-aging, antitumor, anti-alzheimer disease, treating.The size of T mono-AOC can reflect the body Antioxidant Enzymes state of non-enzyme system to the compensatory capacity of external irritant and body Radical Metabolism of unifying, and is the good index of reflection body anti-oxidation function.SOD, as antioxidase in body, is oxygen free radical scavenger in body, and Cell protection is avoided damage, to maintaining Radical Metabolism balance, plays an important role.CAT can decompose hydroxyl free radical makes its lowering of concentration, thus the stable interior environment of protection body cell and cell orthobiosis.MDA is the main metabolites of machine interior free yl and unsaturated fatty acid generation lipid peroxidation, often be used to indirectly to reflect the variation of the Radical Metabolism of body, it has serious damaging action as the main metabolites of body inner lipid peroxidization to cell.

Vitamin C, vitamin E

Vitamin c: vitamin C claims again ascorbic acid, is water soluble antioxidant, is also that in blood plasma, the most effective antioxidant is easily oxidized, and copper and the ferrum of meeting air, heat, light, alkaline matter, oxidase and denier all can accelerate its Oxidative demage.As a kind of powerful antioxidant, its maximum characteristic is reproducibility, eliminates the toxicity of harmful oxygen-derived free radicals by reduction.Its antioxidation show can with O2.、HOO。And OH.Reaction, generates half hydroascorbic acid rapidly, removes singlet oxygen, reduced sulphur free radical, and its antioxidation relies on reversible dehydrogenation reaction to complete.Because it is hydrogen donor, also can make oxidized vitamin E and sulfydryl revert to reduced form, this is its indirect antioxidation.The report vitamin c interior antioxidation actions such as Dai De are better than external, and it may bring into play antioxidation by other indirect approach in vivo, and is not only direct removing oxygen-derived free radicals.The not only Wheat Protein of disorderly C of supporting one's family, also has prooxidant effect under certain condition.The enol form hydroxyl of ascorbic acid has acidity, under Transition-Metal Ions (especially ferrum and copper) exists, can produce OH mono-, but ascorbic acid when removing OH mono-can produce again half hydroascorbic acid anion free radical, when half hydroascorbic acid anion free radical (A mono-) can not be removed in time, can bring out a series of free radical lock reactions, thereby make the unsaturated fatty acid generation lipid peroxidation in body, body is damaged, and the method that now reduces the c of the stockaded village detrimental effect of supporting one's family mainly contains the consumption and the unit interval that 1. regulate and control vitamin c and cheats concentration; 2. vitamin E is provided; 3. GSH is provided; 4. aminoacid is provided.The rope c that supports one's family can be used for flu, defying age, cancer-resisting, resisting hypertension, antiallergic, cataract etc. clinically 1.With respect to supporting one's family, grasp E; vitamin C is stronger from the free radical ability in the smog of contaminated air and Nicotiana tabacum L. to eliminating; cut the reports such as coral 50 μ mol/L vitamin Cs to pieces and can protect rat lymphocyte; alleviate the oxidative stress that CSS (Cigarette smoke solution) causes; but the joint study group of He Laban hospital of Haifa, Israel Polytechnics finds smoking meeting and makes the vitamin C in saliva change performance; become healthy harmful material, team of institute also needs further exploration to this.Marvin's great waves etc. are reported in vitamin C chronic administration; during rat acute stress state, Brain Tissue SOD Vigor has no obvious change; prompting vitamin C has very strong anti-radical action, and base car can be eliminated the infringement of radical pair neurocyte, thereby has neuroprotective.

1, VE is on being bred as the impact of the clear immune indexes of Sanguis Anas domestica

By table 10 know can, it is higher that serum IgM, IgG, T3 and IL-2 concentration be take respectively III and Group IV (VE addition as 15 and 20IU/kg), minimum is the 1st group, but difference not significantly (P > 0.05).From table, data also can be found out, IgM, IgG, T3 and IL 1 can have rising in various degree along with the increase of VE addition, but the too high trend that presents reduction of addition.And level of serum cortisol the highest be the 1st group, lower is III and Group IV, has reduced by 10.94% and 13.90% respectively than the 1st group, but difference not significantly (P > 0.05).From table, data can be found out, each organizes level of serum cortisol along with the increase of VE addition has reduction in various degree.

The VE of table 11 varying level is on being bred as the impact of the clear immune indexes of Sanguis Anas domestica

2, VE is on being bred as the impact of duck Serum antioxidant indices

As can be seen from Table 4, the activity of serum T-AOC and GSH-Px is higher with III and Group IV respectively, but each group difference not significantly (P > 0.05).The activity of SOD in serum, the 1st group has been reduced by 37.97% and 40.31% than III and Group IV respectively, Group II has reduced respectively 32.85% and 35.39% than III and Group IV, significant difference (P < 0.05), but with Group V and Group VI difference not significantly (P > 0.05).In table, data can be found out, the activity of serum T-ACC, SOD and GSH-Px has rising in various degree along with the increase of VE addition, but too much its activity of addition has the trend of reduction.What in serum, the content of MDA was the highest is the 1st group, lower is III and Group IV (15 and 20IU/kg), than work group, reduced respectively 7.39% and 11.86%, but difference is significantly (P > 0.05) not, the content of serum MDA reaches minimum and then with the increase of addition, is the trend rising gradually when adding 20IU/kg VE.

The VE of table 12 varying level is on being bred as the impact of duck Serum antioxidant indices

3, VE is on being bred as the impact of the dirty Antioxidant Indexes of duck liver

From table 5, the activity of liver T-AoC, SoD and GSH-Px is higher with III and Group IV, but there was no significant difference (P > 0.05) between each group.In table, data can be found out, the activity of liver T-AoC, SOD and GSH-Px has rising in various degree along with the increase of VE addition, but too much its activity of addition has the trend of reduction.The content of MDA in liver, wherein Group IV (20IU/kg) has significantly reduced by 60.23% (P < 0.05) than the 1st group (OIU/kg), but each group of Group IV and other is compared difference not significantly (P > 0.05), the content that simultaneously also can find out liver MDA reaches minimum and then with the increase of addition, is the trend rising gradually when adding 20IU/kg VE.

The VE of table 13 same level is on being bred as the impact of the dirty Antioxidant Indexes of duck liver

Fructus Lycii

The mature fruit of lycium barbarum is rich in special bioactive functions composition, as kryptoxanthin, cryptoxanthin, physalin, betanin, scopolamine, taurine, r aminobutyric acid, lycium barbarum polysaccharide etc. anoxia and fatigue are the physiological and biochemical procedures of animal body complexity, about the test of Fructus Lycii anoxia enduring and antifatigue effect has been reported, also have evidence Folium Lycii tea, boxthorn seed oil can significantly strengthen recessed the crying of hypoxia-bearing capability of mice]. wherein, lycium barbarum polysaccharide is considered to the main functional health factor of Fructus Lycii, is also a maximum component in Fructus Lycii efficacy study report, has and protects the liver, high temperature resistance, radioprotective, anti peroxidation of lipid, slow down aging, anti-cancer and inhibiting tumor, blood sugar lowering, immunomodulating and fatigue-resisting function. there are some researches show, why Fructus Lycii has anoxia enduring and anti-fatigue ability, owing to itself containing complicated medicinal ingredient. the evidence of Wei Zhiqing etc., taurine and r aminobutyric acid all can improve the anti-anoxia ability of animal. according to another report, and lycium barbarum polysaccharide, betanin, the compositions such as B-carotene are also the active substance of Fructus Lycii oxygen lack resistant function. the medical value of lycium barbarum, develop new anti-hypoxia, anti-fatigue Chinese medicine provides test basis.

1, the impact of Fructus Lycii water decoction on the mice airtight anoxia time-to-live

Fructus Lycii water decoction affects result of the test in Table the different difference extremely significantly (P < 0.01) that represents of same column capitalization in 14. tables 14 to the mice airtight anoxia time-to-live, the different significant differences (P < 0.05) that represent of lower case. as shown in Table 1, the Fructus Lycii water decoction of doses has obvious oxygen lack resistant function to mice. between the Fructus Lycii water decoction group of 2 dosage of height, reached difference utmost point significant level (P < 0.01), between high dose Fructus Lycii water decoction gavage group and matched group, difference has also reached utmost point significant level (P < 0.01).

The impact of table 14 Fructus Lycii water decoction on the mice airtight anoxia time-to-live

2, Fructus Lycii water decoction is on the swim impact of physical exhaustion time of mice

What Fructus Lycii water decoction was swum the physical exhaustion time to mice affects result of the test in Table the different difference extremely significantly (P < 0.01) that represents of same column capitalization in 15. tables 15, the different significant differences (P < 0.05) that represent of lower case. as shown in Table 15, the Fructus Lycii water decoction of doses has obvious antifatigue effect to mice. between the Fructus Lycii water decoction group of 2 dosage of height, reached difference utmost point significant level (P < 0.01), between 2 dosage groups of height and matched group, difference has also reached utmost point significant level (P < 0.01).

Table 15 Fructus Lycii water decoction is on the swim impact of physical exhaustion time of mice

Show, in the test of mice airtight anoxia, between high dose Fructus Lycii water decoction gavage group and matched group, difference has reached utmost point significant level (P < 0.01). mice, swim in physical exhaustive cropping technique, between the Fructus Lycii water decoction gavage group of 2 dosage of height and matched group, difference has reached utmost point significant level (P < 0.01). and test shows, the Fructus Lycii water decoction of doses has the effect of significant anoxia and fatigue-resisting to mice. and Fructus Lycii water decoction is to the significant anti-hypoxia of mice, the antifatigue effect abundant bioactive substance contained with Fructus Lycii is relevant. and Many researchers is studied wolfberry fruit extract and active component thereof, lycium barbarum polysaccharide (lycium barbarum polysaccharide, LBP) improving the adaptive capacity of body to sports load, resisting fatigue generation and the elimination aspect accelerating fatigue have fairly obvious effect. and the test group that Luo Qiong etc. are divided into 5 various dose by sterling LBP is filled with hello mice, proof LBP can significantly increase the lactic acid dehydrogenase total activity of mice, the metabolite of body while effectively removing strenuous exercise, by increasing the deposit of glycogen, for providing more energy, body reaches the object of resisting fatigue, thereby the generation of delay fatigue, the elimination accelerating fatigue. find in the test of impact of activities of antioxidant enzymes on oxidative Ability of Sport Training Mouse endurance and body observing LBP, LBP can obviously improve the activity of glutathione peroxidase GSH-Px in the SOD activity of oxidative Ability of Sport Training Mouse blood plasma and muscular tissue and hepatic tissue, thereby be conducive to the removing of active oxygen, the generation of delayed motion fatigue and athletic injury. the motion of body mainly carrys out energy supply by glycolysis, main metabolites is blood lactic acid, prolonged exercise exhausts sugar deposit, cause blood lactic acid to be piled up, to cause tired major reason, therefore improve sugar deposit significant to improving motor capacity. after the energy balance is destroyed, along with the adaptability reduction of body movement load and the reinforcement of protein and the effect of nitrogen-containing compound catabolism, blood urea nitrogen increases just more obvious. and LBP can improve the energy metabolism of body just, accelerate the decomposition energy supply of hepatic glycogen, reduce the decomposition of protein and nitrogen-containing compound, thereby reduction blood urea nitrogen, there is antifatigue effect.

3, serolysin test, antibody generates test and shows: middle and high dosage group is compared with matched group, middle and high dosage group antibody product significantly increases, high dose group hemolysis plaque obviously increases, high dose group auricle swelling degree and middle and high dosage group lymphocyte transformation test absorbance difference significantly increase, the phagocytic index of high dose group monokaryon one macrophage significantly increases, and high dose group NK cytoactive significantly increases.Cellular immune function: each organizes mice auricle swelling increases gradually with dosage, and high dose group swelling is compared difference with matched group significance, and middle and high dosage group adds with the absorbance difference difference that does not add ConA significance.

Table 16 delayed allergy DTH and lymphocyte transformation test

4, humoral immune function:

Table 17 is visible, and middle and high dosage group antibody product significantly increases, and hemolysis plaque digital display work increases.

Table 17 serum hemolysin and hemolytic plaque test

5, macrophage function: macrophage phagocytic index all increases with dosage value added, and the increase of high dose group phagocytic index also there were significant differences; NK cytoactive increases with dosage, and particularly high dose is active increases significantly.

Table 18 carbon is cleaned up macrophage phagocytic exponential sum NK cytoactive detection

Select ICR mice, from different angles such as cellular immunization, humoral immunization, nonspecific immunities, study respectively, obtain more consistent result.Result shows that Fructus Lycii can obviously strengthen macrophage phagocytic function.Macrophage plays an important role in body nonspecific immunity and specific immunity, and it has process antigen, submission antigen, engulfs the function of pathogen.Also secrete some media and participate in body specific immune response and immunomodulating.After bone-marrow-derived lymphocyte is stimulated by antigen (SBRC), be divided into plasma cell and produce antibody, when again accepting same antigen, haemolysis forms macroscopic plaque, this hemolysis plaque number can reflect antibody forming cell's number substantially, so hemolysis plaque number is more, illustrate that antibody forming cell's number is more.Structure is offered sacriffices to the gods or the spirits of the dead the hemolysis plaque showed increased that sub-high dose group can make mouse boosting cell, showing that structure is offered sacriffices to the gods or the spirits of the dead can obviously strengthen the humoral immunization of mice, T lymphocyte is mainly born cellular immune function, and it and delayed allergy, transplant rejection, tumour immunity etc. have substantial connection.In the mouse lymphocyte conversion test and delayed allergy (DTH) of ConA induction, high dose group is compared with matched group, has significant difference in this experiment.Show that structure offers sacriffices to the gods or the spirits of the dead the cellular immunity that son can strengthen mice.Structure is offered sacriffices to the gods or the spirits of the dead son the immunologic function of mice is had to obvious regulating action, and along with the increase of dosage, its function has enhancing trend.

Radix Ginseng

Radix Ginseng in formula is included and in < < Ministry of Public Health, about the notice > > adnexa 2 of further standard healthy food material management, be can be used for the article list of health food, the more bibliographical information of Radix Ginseng Radix Ginseng there is enhancing immunity function, in " research that Folium Ginseng Saponin regulates chicken immuological function " literary composition of delivering on < < Agriculture of Anhui science > > such as people such as Luo Yanqiu, experiment conclusion is thought: Folium Ginseng Saponin can promote the generation of chicken body internal specific antibody and the phagocytic activity of activated mononuclear-macrophage system, " ginsenoside's immunomodulating and Studies On Antiviral Activities " literary composition that Chu Xiuling delivers on < Chinese veterinarian < medical magazine > > is thought: ginsenoside can promote the growth of Immune Organs of Body, thereby directly affects immunity of organisms, ginsenoside also can affect each stage of hematopoietic cell CFU-GM and hematopoietic stem cell, thereby strengthens the function of immunocyte, improves the resistance of body, in rich " impact of Ginsenoside Rg3 on the immunologic function " literary composition that waits people to deliver on the journal > > of < < China Medicine University of imperial court, test and show: Ginsenoside Rg3 can obviously improve mice carbon clearance speed, immune organ weight, serum hemolysin content, splenocyte transforms and N K is active, illustrates that Ginsenoside Rg3 has the effect that improves immunologic function, in " ginsenoside Rg1's immunologic enhancement " literary composition that the people such as Ren Jiehong deliver on < < new Chinese medicine and clinical pharmacology > >, experimental result shows: ginsenoside Rg1 can increase the weight of mouse immune organ and the phagocytic function of macrophage, improves the content of IL-2, complement C3, C4 in rat blood serum.Illustrate that Rg1 can improve laboratory animal immunity of organisms, play the effect that improves body defense function, have immunologic enhancement.

1, the impact of PDS on experimental remodeling ventricle rat plasma Ang II, ET content and myocardium Ang II content

With sham operated rats comparison, reconstruction model group rat plasma Ang II, ET content and myocardium Ang II content all obviously increase (P < 0.01), rat plasma Ang II, ET content and myocardium Ang II horizontal abnormality while showing remodeling ventricle.PDS can significantly reduce rat plasma Ang II, ET content and myocardium Ang II content (P < 0.01), and its effect is suitable with positive drug captopril, in Table 19.

The impact of table 19PDS on experimental remodeling ventricle rat rat plasma Ang II, ET content and myocardium Ang II content

2, the impact of PDS on experimental remodeling ventricle rat plasma ALD, ANP content

With relatively reconstruct model group rat plasma ALD and all obviously increases (P < 0.01) of ANP content of sham operated rats.Rat plasma ALD and ANP horizontal abnormality while showing remodeling ventricle.PDS can significantly reduce rat plasma ALD and ANP content (P < 0.01), and its effect is suitable with positive drug captopril, in Table 20.

The impact of table 20PDS on experimental remodeling ventricle rat rat plasma slurry ALD and AANP content

3, the impact of PDS on experimental remodeling ventricle rat blood serum MDA content and SOD, GSH-Px activity

Compare with sham operated rats, reconstruction model group Content of MDA obviously increases (P < 0.01), and active obviously reduce (the P < 0.01) of SOD, GSH-Px can produce oxygen-derived free radicals and cause myocardial damage while showing remodeling ventricle.PDS can obviously reduce Content of MDA (P < 0.01) and improve SOD in serum, GSH-Px active (P < 0.05), its effect is suitable with positive drug captopril, show that its Free radicals injury that ventricular remodeling in rats is caused has obvious antagonism, in Table 21.

The impact of table 21PDS on experimental remodeling ventricle rat blood serum MDA content, SOD and GSH-Px activity

Originally studies show that, ventricular remodeling after myocardial infarction group rat plasma Ang II and ALD content, myocardium Ang II content obviously raises, and shows renin angiotensin aldosterone system increased activity.Ang II, as the effector molecule of renin angiotensin aldosterone system, can directly promote the synthetic of myocardial cell RNA and protein, promotes fibrocyte propagation, causes remodeling ventricle.And excessive ALD activates and enters nucleus after corresponding receptor and be combined with specific DNA sequence, regulate transcribing of a series of ALD response genes, regulate and control the synthetic of some protein, performance genome effect, stimulating cellular growth and collagen remodeling, also can cause myocardial hypertrophy and myocardial fibrosis.In addition, this experimental model group rat plasma ET and ANP content also obviously raise, and this is consistent with the result of bibliographical information.PDS can significantly reduce blood plasma ET, ANP, ALD content, blood plasma and myocardium Ang II content, thereby prompting PDS suppresses renin-angiotensin-aldosterone system reduces vaso-excitor material level, may be that its mechanism of bringing into play anti-remodeling ventricle effect is when remodeling ventricle, because ventricle wall tension force increases, relative myocardial ischemia anoxia, a large amount of mesostates is piled up, free radical (as MDA) is produced and increase, in body, Antioxidant Enzymes (SOD and GSHPx) activity is suppressed simultaneously.Originally studies show that, after rat myocardial infarction model 4 weeks, Content of MDA increases, and SOD, GSH-Px are active, obviously reduce, prompting body oxidation/antioxidative enzyme system balance has been broken, and in body, oxygen is freely removed scarce capacity, cause myocardial cell injury, fibroblast proliferation, myocardial fibrosis, remodeling ventricle forms.After continuous 4 weeks intraperitoneal administrations of PDS, rat vivo oxidation and antioxidative poised state significantly improve, and the effect of pointing out PDS to delay remodeling ventricle development may be relevant with the oxidation resistance of its enhancing cardiac muscle.

Semen Ginkgo

Semen Ginkgo is Ginkgoaceae Ginkgo deciduous tree for many years, has very high development and application values.Yet mesosperm of Ginkgo biloba is mostly dropped as waste material in the course of processing, cause the wasting of resources and environmental pollution.Therefore, be necessary the effective ingredient of mesosperm of Ginkgo biloba to study, so that comprehensive utilization gingko resource is preserved the ecological environment.Many studies confirm that in recent years, Flavonoid substances has the ability that suppresses lipid peroxidation and remove multiple free radical, is a kind of comparatively desirable Natural antioxidant.Research is found to contain Flavonoid substances in mesosperm of Ginkgo biloba extracting solution, and has obvious antioxidant activity.This research adopts respectively the methods such as microwave, ultrasonic, ethanol and flooding to extract the Flavonoid substances in mesosperm of Ginkgo biloba, measured the antioxidant activity of different extracting solution in lipovitellinin polyunsaturated fatty acid peroxidating system, and remove ultra-oxygen anion free radical, hydroxy radical and hexichol for the ability of bitterness free acyl radical, measured the total antioxidant capacity of different extracting solution, for the comprehensive development and utilization of mesosperm of Ginkgo biloba provides theoretical foundation simultaneously.

1, the impact on lipovitellinin PUFA peroxidating system antioxidant activity

1.1 incubative time incubations are to bring out the snperoxiaized important step of lipovitellinin.This research is on the basis of bibliographical information, and the 0.1200mg/mL rutin solution of first take adds in lipovitellinin PUFA peroxidating system as antioxidant, the relation of having observed incubative time and antioxidant activity.Result (Fig. 1) demonstration, at initial period, rutin is in rising trend to the suppression ratio of lipovitellinin PUFA peroxidating system.When reaction system incubation 30min, rutin is maximum to the suppression ratio of lipovitellinin PUFA peroxidating system, is 46.09%; Along with the continuation of incubative time extends, suppression ratio declines rapidly, tends towards stability to 4h.Therefore in follow-up test, using 37 ℃ of incubation 30min as the reaction condition of evaluating mesosperm of Ginkgo biloba extracting solution antioxidant activity.

1.2 different extracting solution are to being diluted to by volume P1 and the P22 kind extracting solution of variable concentrations, and the antioxidant activity of vitamin C and rutin solution compares, and the results are shown in Figure 2.As shown in Figure 2,4 kinds of solution of variable concentrations all show obvious antioxidant activity, and its antioxidant activity is with the increase of concentration (presenting dose-effect relationship) in rising trend.Wherein, the highest with the antioxidant activity of undiluted alcohol extract (P1), the suppression ratio of lipovitellinin PUFA peroxidating system is reached to 50.69%, Microwave Water extract (P2) takes second place, and suppression ratio is 34.12%.The Antioxidation Mechanism of mesosperm of Ginkgo biloba extracting solution relates to two aspects: the one, and Flavonoid substances can generate complex with the Fe2+ with promotion peroxidation, thereby reduces the catalytic activity of Fe2+; The 2nd, in the middle of Flavonoid substances can offer hydrogen, free radical RO-and ROO etc., self change stable phenolic group free radical into, slows down the speed of free free chain reaction, thereby play the further effect of oxidation of lipovitellinin that suppresses.And alcohol extract to the suppression ratio (50.69%) of lipovitellinin PUFA peroxidating system far away higher than the standard rutin solution (23.42%) of approximate concentration, the multiple Flavonoid substances that mesosperm of Ginkgo biloba extracting solution contains is described, and there is cooperative effect between other antioxidants.

2, Cd single and with the impact of SOD Combined Stress on Semen Ginkgo activity

The Semen Ginkgo SOD activity change of normal growth is relatively mild, whole trophophase is in comparatively stable level. but after Cd processes 30d, 50d compared with the control the SOD activity of each level of Cd be all the trend of rising. and after being increased to 82d with the increase of coercing concentration, SOD activity reaches peak, Cd150, Cd100, Cd50 are significantly higher than 110.99%, 66.37%, 42.88% of concurrent control, but all obviously decline subsequently, high concentrations of Cd 150 declines the most remarkable, and during to 128d, SOD activity is only the 71.04%. of contrast

In each level of Cd, affect main manifestations for promote or coordinate repression to SOD activity adding of pb: as added after Pb in Cd varying level, when W1, after Stress treatment to 82d Semen Ginkgo SOD activated water on average higher than contrast, but it is not remarkable with the level difference of corresponding single Cd effect, when W2 and W3, SOD activated water on average reaches significant difference higher than contrast and single Cd to coercing 82d, and high concentrations of Cd 150 amplification are particularly evident, and Pb shows as collaborative trend to the effect of cd.Along with the prolongation of the time of coercing, each SOD activity of processing declines gradually, and obviously declines with the raising of stress level later, and to 128d, all significantly lower than contrast and corresponding single Cd level, adding of pb shows as inhibitory action to the effect of SOD activity.

3, DE single and with the impact of pb Combined Stress on Semen Ginkgo CAT activity

The Semen Ginkgo CAT activity change of normal growth is relatively mild, and whole trophophase is substantially in metastable level, 30d, 50d after Cd processes, the CAT activity change rule of each level of Cd is not obvious, lower than or approach the control level of the same period, illustrating that cd processes does not still bring out the defense reaction of CAT, H ₂o ₂the free radical accumulation causing is also not obvious, but after this with the increase of coercing concentration, the CAT activity of different disposal sharply rises, and reaches peak to CAT activity after 82d, and Cd150, Cd100, Cd50 are significantly higher than 102.54%, 82.54%, 33.71% of concurrent control.After 82d. the CAT activity that different Cd process significantly declines. and high concentrations of Cd 150 declines.

Tea polyphenols

Folium Camelliae sinensis has the plantation of several thousand and drinks history in the world. and having tea makes for good health is accepted by people, to have tea and can prevent some diseases to be also confirmed by experiments. research shows, in Folium Camelliae sinensis, to the most useful healthy material, be to there is the very tea polyphenols of strong anti-oxidation ability. it can effectively remove oxygen-derived free radicals and Lipid Free Radicals, prevention lipid peroxidation, and have and suppress tumor and occur, the functions such as slow down aging. with regard to tea polyphenols, remove oxygen-derived free radicals and one elaboration of antioxidative molecule mechanism do herein, exploitation and utilization that hope promotes the further investigation of tea polyphenols antioxidation mechanism and expands tea polyphenols with this, serve the healthy and long-lived of the mankind.

1, the scavenging action of tea polyphenols to oxygen-derived free radicals

Oxygen generates water process in reduction and produces superoxide anion hydrogen peroxide hydroxyl radical free radical and singlet oxygen. these active oxygens play an important role in lipid peroxidation process. a kind of antioxidant can antioxidation and antioxidation efficiency how, very important character is exactly to see its scavenging action to oxygen-derived free radicals in different systems.

2 tea polyphenols are removed the synergism of oxygen-derived free radicals

Tea polyphenols mainly comprises 4 kinds of monomer components, these 4 kinds of monomers have synergism and best removing ratio to the removing of oxygen-derived free radicals. with chemoluminescence method and ESR commercial measurement, show, generally have compared with the catechin of polyphenol hydroxyl and there is stronger oxidation resistance, the position of phenolic hydroxyl group and gallic acid esterification also have a great impact the people such as .Jovanovic [6] to its oxidation resistance and with radiolysis, have measured tea polyphenols and found mensuration antioxidant activity technology, measured the antioxidation gesture of tea polyphenols, all having obtained similar result. catechin combination of two has collaborative scavenging action to ultra-oxygen anion free radical, when EGCG and ECG exist simultaneously, the synergistic function of removing ultra-oxygen anion free radical is maximum, secondly be ECG and EC combination and EC and EGC combination. the potentiation of combination of two, neither form catechin clearance rate simply add and, relation neither multiply each other, but be obviously better than the combination of two kinds of catechins with the combination potentiation that the molar concentration of catechin is the positive correlation .3 kind catechin of height, and synergistic effect is consistent with relation and the combination of two of concentration, combination potentiation with EGCG+ECG+EC is the most obvious. according to surveying and determination, in fresh Folium Camelliae sinensis, the general proportions of these 4 kinds of catechins is EGCG: ECG: EGC: EC=5: 2: 2: although 1. the ratio of EGCG has comparative advantage, but its synergistic effect is not as natural combination [5]. this results suggest, usining catechin during as antioxidant, to keep its antioxidant effect of original catechin ratio best. to be perhaps Camellia sinensis form through selection for many years for this, it is the result that Camellia sinensis is evolved, also be the natural defense system that Camellia sinensis opposing nature adverse circumstance forms. Liu Zhongli professor laboratory research tea polyphenols in homogeneous phase solution micelle human ldl and erythrocyte membrane non-oxidizability and with the synergism of vitamin E. find that tea polyphenols is all effective antioxidant in above-mentioned system, its non-oxidizability is relevant to the micro environment of the penetrance of film and medium with the oxidation-reduction potential molecule of molecule. and they have distinguished measurements and calculations, and tea polyphenols and vitamin E suppress the time speed of peroxidization and measure the factor, and in chain growth the kinetic chain length with the chain inhibition phase. the antioxidant activity of finding EGCG and ECG is close from vitamin E. but the response feature of the two is different in SDS and CTAB micelle. in CTAB micelle their inhibitory stage and measure the factor all than SDS in greatly, but reaction rate is lower. this shows that micellar surface electric charge measures and studies show that the tea polyphenols antioxidant activity .ESR that plays an important role, tea polyphenols and vitamin E have synergism when removing water free radical at micelle and biofilm surface removing Lipid Free Radicals and cutting off free chain reaction

The scavenging action of the oxygen-derived free radicals that 3, tea polyphenols produces myocardial ischemia-reperfusion

We have studied tea polyphenols scavenging action to oxygen-derived free radicals in myocardial ischemia-reperfusion system. and ischemical reperfusion injury is to detect a good model organizing ischemia-reperfusion to produce oxygen-derived free radicals, be commonly used to check medicine at tissue ischemia Reperfusion injury damaging the spleen and stomach to the removing of oxygen-derived free radicals with to myocardium protective effect. adopt isolated rat heart, by Longerf method, heart is carried out to ischemia-reperfusion. result shows simple ischemia, oxygen-derived free radicals is not high, and after ischemia-reperfusion, oxygen-derived free radicals increases by 188% left and right. in infusion liquid, adding tea polyphenols again, oxygen-derived free radicals obviously reduces, and there is obvious concentration dependence.

4, the inhibitory action of tea polyphenols to Peroxynitrite oxidation activity

NO and ultra-oxygen anion free radical have very high reaction rate constant (6.4109mol L-1 s-1), form the extremely strong material Peroxynitrite (ONOO-) of oxidisability, oxidable cell membrane lipid and protein, cause the generation of cell injury and disease. under alkali condition, Peroxynitrite is more stable, once protonated, decompose to produce immediately hydroxyl and NO2 free radical. in a lot of pathological states, there is this type of reaction. with the research of spin trapping technique find tea polyphenols the inhibitory action of ONOO-oxidation activity is obviously greater than the people such as Quercetin and vitamin C [13] .Pannala study discovery ECG and EGCG to the removing successful of Peroxynitrite higher than EC and EGC.

Microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate

Microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate is tablet processing adjuvant, for the operability of the tablet course of processing, we have selected food additive: microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate is tablet processing adjuvant, according to the Zhu Yu of Guangzhou Inst of Chemistry, Chinese Academy of Sciences qin, Tang Liegui, in one one kinds of multi-functional macromolecular material > > mono-literary compositions of < < microcrystalline Cellulose that Pan Songhan delivers on the 3rd phase at < < Chemical Industry in Guangzhou > > the 21st volume in 1993, show: microcrystalline Cellulose can be used as antiplastering aid.Microcrystalline Cellulose addition is 15% of formula, meet GB2760 < < food additive and use sanitary standard > >, microcrystalline Cellulose toxicity ADI does not do particular provisions (FAO/WHO, 1994); Make like this its whole formula have more reasonability and science.

The health medicine of a kind of enhancing immunity, anti-senescence function; it is characterized in that; comprise Caulis et Folium Brassicae capitatae, taurine, vitamin C, vitamin E, Fructus Lycii, Radix Ginseng, Folium Ginkgo extract, tea polyphenols, microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate, each component ratio is: Caulis et Folium Brassicae capitatae 20g, taurine 1g, vitamin C 1g, vitamin e1 g, Fructus Lycii 15g, Radix Ginseng 9g, Folium Ginkgo extract 12g, tea polyphenols 4g, microcrystalline Cellulose 9g, dextrin 8g, pregelatinized Starch 6g, Icing Sugar 8g, polyvidone 1g, magnesium stearate 1g.

This pharmaceutical formulation ratio is: Caulis et Folium Brassicae capitatae 9-20g, taurine 0.4-1g, vitamin C 0.4-1g, vitamin E 0.4-1g, Fructus Lycii 9-15g, Radix Ginseng 4.5-9g, Folium Ginkgo extract 9-12g, tea polyphenols 2.2-4g, microcrystalline Cellulose 3.6-9g, dextrin 3.1-8g, pregelatinized Starch 2.5-6g, Icing Sugar 2.5-8g, polyvidone 0.4-1g, magnesium stearate 0.3-1g.

First Fructus Lycii, Radix Ginseng medicine are pulverized respectively, crossed 100-120 mesh sieve, get respectively supplementary material and weigh by a certain percentage, carry out supplementary material mixing, incorporation time 45-50 minute, then carry out tabletting, within control strip focuses on inner quality standard.Carry out next procedure screening, polishing, inner packing, outer package, finally by inner quality standard check warehouse-in.

Through verification experimental verification; wherein, in above-mentioned recipe ratio, preferably embodiment one is: Caulis et Folium Brassicae capitatae 9g, taurine 0.4g, vitamin C 0.4g, vitamin E 0.4g, Fructus Lycii 9g, Radix Ginseng 4.5g, Folium Ginkgo extract 9g, tea polyphenols 2.2g, microcrystalline Cellulose 3.6g, dextrin 3.1g, pregelatinized Starch 2.5g, Icing Sugar 2.5g, polyvidone 0.4g, magnesium stearate 0.3g.

Preferably another embodiment is: Caulis et Folium Brassicae capitatae 20g, taurine 1g, vitamin C 1g, vitamin e1 g, Fructus Lycii 15g, Radix Ginseng 9g, Folium Ginkgo extract 12g, tea polyphenols 4g, microcrystalline Cellulose 9g, dextrin 8g, pregelatinized Starch 6g, Icing Sugar 8g, polyvidone 1g, magnesium stearate 1g.

After above-mentioned processing technique, in medicine of the present invention, preferably active constituent content distributes as follows: 100g is containing the sweet 730mg of effective ingredient Radix Raphani sulfur, polysaccharide 3700mg, total saponins 1800mg.Effective ingredient in Fructus Lycii is polysaccharide, and the effective ingredient in Radix Ginseng is saponin, and the effective ingredient in Folium Ginkgo is flavone.

The present invention finds through test, the medicament being mixed according to the above ratio has the effect of the enhancing body immunity slow down aging better than simple one or both compositions, and is overcome through the adverse effect that the proportioning of above-mentioned various composition specific proportions has beat all health-care effect and former each composition.

Especially more preferably, during this production, supplementary material mixing time 45-50 minute is row tabletting again, the results showed, the long or too short drug effect for last preparation of incorporation time of supplementary material has adverse effect.45-50 minute for guaranteeing that final drug effect has important function.

More than this specific embodiment several raw materials are mutually auxiliary in whole formula deposits mutually, bring out the best in each other, make full side present good effect, meet the composite reason of Chinese medicine, product has obvious enhancing immunity and obvious deferring senility through function test proof this product.

Claims (1)

1. the health medicine of an enhancing immunity, anti-senescence function; it is characterized in that; comprise Caulis et Folium Brassicae capitatae, taurine, vitamin C, vitamin E, Fructus Lycii, Radix Ginseng, Folium Ginkgo extract, tea polyphenols, microcrystalline Cellulose, dextrin, pregelatinized Starch, Icing Sugar, polyvidone, magnesium stearate, each component ratio is: Caulis et Folium Brassicae capitatae 20g, taurine 1g, vitamin C 1g, vitamin e1 g, Fructus Lycii 15g, Radix Ginseng 9g, Folium Ginkgo extract 12g, tea polyphenols 4g, microcrystalline Cellulose 9g, dextrin 8g, pregelatinized Starch 6g, Icing Sugar 8g, polyvidone 1g, magnesium stearate 1g.