TWI440465B - Herbal extract mixture for reducing blood lipid and a combination thereof - Google Patents

Description

Chinese herbal medicine extract mixture for reducing blood fat and pharmaceutical composition thereof

The present invention relates to a Chinese herbal medicine extract mixture, in particular to a Chinese herbal medicine extract mixture for lowering blood fat and a pharmaceutical composition thereof.

After the social type of business is transferred from agriculture to commercial activities, due to the reduction of activity and changes in diet, modern people's caloric intake is too much, causing fat to accumulate in the body, causing modern people to have high blood lipids. Once the blood lipid level in the body is high, fat will accumulate in the body, and even lead to fatty liver or atherosclerotic arteriosclerosis, and then complicated by cardiovascular diseases such as stroke, arteriosclerosis or heart disease. It can be seen that hyperlipidemia is the main cause of cardiovascular disease, and it should be strictly controlled that blood lipids do not exceed the normal range to reduce the incidence of cardiovascular disease.

In addition to cultivating good living habits, minimizing caloric intake and increasing daily exercise, according to the Tzu Chi Hospital's 2004 Chinese Medicine Journal, "The Health of the Silver-Care" - Hyperlipidemia In the article "Chinese medicine", it is recommended that patients with hyperlipidemia can choose the appropriate natural Chinese herbal medicine according to their high blood lipid type to control the blood lipid content in the body.

According to the content of the article, tannin, scorpion and safflower have the effect of inhibiting fat accumulation, and there are many natural Chinese herbal medicines or foods with hypolipidemic functions such as oats, ganoderma, coral grass and acanthopanax. Or yam, etc. However, due to the low content of active ingredients in various natural Chinese herbal medicines or foods, even if hyperlipidemia patients consume these Chinese herbal medicines or foods every day, they cannot ensure that the intake of such Chinese herbal medicines or foods by the consumers is sufficient to inhibit blood lipid storage. The active ingredient of the accumulated cells.

Furthermore, in order to increase the active ingredient content of various natural Chinese herbal medicines, various active ingredients are further extracted to obtain herbal extracts thereof. For example, Taiwan Public Publication No. 200920390 "oil-soluble extract of herbal medicine with blood lipid regulation function" is selected from the group consisting of nutmeg, licorice, Chuanxiong, Schisandra, Shanzhi, Angelica, Danshen, Atractylodes, Sanqi, Rehmannia, Huaishan, Eucommia, A group consisting of ginkgo, tangerine peel, puerarin, huangjing, medlar, purslane, cassia seed and mixtures thereof, is extracted with an oil-soluble solvent to obtain a Chinese herbal oil-soluble extract. However, the combination of the oil-soluble extract of the Chinese herbal medicine has a wide variety, but it is impossible to confirm whether the active ingredients of the respective combinations of the Chinese herbal medicines interfere with each other or inhibit the blood lipid lowering effect.

The main object of the present invention is to provide a Chinese herbal medicine extract mixture for lowering blood fat, which is capable of effectively reducing the risk of cardiovascular and cardiovascular diseases.

A second object of the present invention is to provide a Chinese herbal medicine extract mixture for lowering blood fat, wherein each Chinese herbal medicine extract has a multiplier for inhibiting fat accumulation.

Still another object of the present invention is to provide a pharmaceutical composition for lowering blood fat, which comprises three kinds of herbal extracts capable of synergistically acting on each other to enhance the blood fat lowering effect.

In order to achieve the foregoing object, the technical content of the present invention comprises: a Chinese herbal medicine extract mixture for lowering blood fat, comprising: a weight percentage of 33.4 to 77.7% of coral grass extract; 11.14~33.3% of acanthopanax extract; and a percentage by weight of 11.14~33.3% of yam extract.

Wherein, the coral grass extract, the acanthopanax extract and the yam extract are preferably obtained by the following steps: an extraction step, using water as an extraction solvent, respectively for the coral grass sample, the acanthopanax sample And extracting the yam sample to obtain a coral grass extract, a acanthopanax extract and a yam extract, wherein the extraction solvent is respectively proportional to the weight ratio of the coral grass sample, the acanthopanax sample and the yam sample It is 1:8~1:12; in a concentration step, the coral grass extract, the acanthopanax extract and the yam extract are separately concentrated to obtain a coral grass extract and a acanthopanax extract. And a yam extract; and a mixing step, mixing the coral grass extract, the acanthopanax extract with the yam extract into the Chinese herbal extract mixture.

The coral grass sample, the acanthopanax sample and the yam sample may be selected as fresh, dried or processed objects that have not been dried or exposed.

The coral grass sample is preferably freeze-dried to obtain a dried coral grass.

The yam sample is preferably dried by heat to obtain a dried yam.

Preferably, the coral grass sample of the extraction step comprises a whole grass part of coral grass.

Preferably, the acanthopanax sample of the extraction step comprises a root or stem of Acanthopanax senticosus.

The yam sample of the extraction step preferably comprises a tuber part of yam.

The temperature of the extraction step is preferably from 60 to 90 °C.

The extraction step is preferably 8 to 10 hours of extraction.

A pharmaceutical composition for lowering blood fat, comprising: a herbal extract mixture according to any one of claims 1 to 10; and a pharmaceutically acceptable carrier or excipient for extracting the herbal extract The mixture is shaped into the pharmaceutical composition.

The dosage of the Chinese herbal medicine extract mixture is preferably 150-600 mg.

Among them, the type of the pharmaceutical composition is preferably a tablet, a capsule, a powder, a granule or a liquid.

The above and other objects, features and advantages of the present invention will become more <RTIgt;

The Chinese herbal medicine extract mixture for reducing blood fat of the present invention comprises a herbal extract from coral grass, acanthopanax senticosus and yam, and the coral grass, the acanthopanax senticosus and the yam herbal extract are mixed in a specific ratio, in particular A mixture of 33.4 to 77.7% by weight of coral grass extract, 11.14 to 33.3% by weight of Acanthopanax senticosus extract and 11.14 to 33.3% by weight of yam extract, mixed with a herbal extract mixture, can be effective Lowering total cholesterol (Total cholesterol, TC for short), Total triglyceride (TG) and Low-density lipoprotein cholesterol (LDL-C), and inhibiting fat cells Fat storage In order to reduce the risk of cardiovascular disease. Coral grass, acanthopanax or yam have the effect of lowering blood lipids, but from the perspective of traditional Chinese medicine, corals are herbally cool, not suitable for long-term use of patients with hyperlipidemia, because of symptoms that may cause digestive discomfort, if they can supplement With the effect of acanthopanax senticosus and yam spleen and stomach, it can reduce the chance of causing body cold by taking coral grass alone, and contribute to the improvement of blood fat lowering effect.

The Chinese herbal medicine extract mixture for reducing blood fat of the present invention can be applied to prepare a hypolipidemic pharmaceutical composition, and the Chinese herbal medicine extract mixture for reducing blood fat of the present invention is combined with a pharmaceutically acceptable carrier or excipient to form a medicine. A composition, which may be in the form of a tablet, a capsule, a powder, a granule or a liquid, and is provided for oral administration by an individual.

Referring to FIG. 1 , the coral grass extract, the acanthopanax extract and the yam extract in the Chinese herbal medicine extract mixture for reducing blood fat of the present invention are prepared by the following method, comprising: an extraction step S1, a Step S2 is concentrated and a mixing step S3 is performed.

The extraction step S1 uses water as an extraction solvent to extract a coral grass sample, a acanthopanax sample and a yam sample, respectively, to obtain a coral grass extract, a acanthopanax extract and a yam extract, wherein The weight ratio of the extraction solvent to the coral grass sample, the acanthopanax sample and the yam sample is 1:8 to 1:12. More specifically, the extraction solvent is extracted with the coral grass sample, the acanthopanax sample and the yam sample at a weight ratio of 1:5, respectively, to improve the extraction efficiency of the extraction solvent without excessive extraction. Solvent to reduce the solvent cost of the extraction step S1.

In this embodiment, the coral grass sample can be selected as the whole grass part of Eucheuma okamurai Yamada, wherein the polysaccharide in the coral grass sample is the main physiological active ingredient, and the molecular weight is 500 Dalton. It is a polymer of monosaccharides such as glucose and xylose, galactose, etc.; the acanthopanax senticosus sample can be selected as the root or stem of Acanthopanax senticosus , wherein the syringin in the acanthopanax senticosus sample is The main active ingredient; the yam sample line can be selected as the root part of Dioscorea alata L.cv.Tainong 1 or D.alata L.cv.Tainong 2, wherein the yam The saponin (Diosgenin) in the sample is the main active ingredient.

More specifically, the Coral Grass Sample, Acanthopanax Senticosus and Yam Samples can be selected as fresh, dried or processed foods that have not been dried or exposed to enhance the active ingredient content of the three Chinese herbal medicine samples. For example, the coral grass sample of the present embodiment is preferably a dried product which has been subjected to freeze-drying treatment for 24 hours; the yam sample of the present embodiment is preferably a dried product which is treated by a heat baking method (hot baking at 50 ° C for 24 hours). This method of preparation contributes to the release of active saponins.

This embodiment is preferably carried out at a temperature of 40 to 60 ° C for 6 to 10 hours to ensure that the active ingredients of each Chinese herbal medicine sample are not destroyed by excessive temperature, and the active ingredients of each Chinese herbal medicine sample can be dissolved therein. In the extraction solvent, in addition, it is preferred to use an oscillating extraction to assist the active ingredients of each Chinese herbal medicine sample to be dissolved in the extraction solvent. Thereby, the active ingredients for lowering blood fat in coral grass, acanthopanax senticosus and yam can be extracted into the extraction solvent, so that each of the extracts can be prepared into a pharmaceutical composition for lowering blood fat.

In this embodiment, water is used as an extraction solvent, and the dried coral grass sample, the acanthopanax sample and the yam sample are respectively extracted at a temperature of 50 ° C, and the extraction time is 8 hours, preferably repeated extraction 3 And remove the remnants of the coral grass sample, the acanthopanax sample and the yam sample, respectively The coral grass extract, the acanthopanax extract and the yam extract are added. Thereby, the active ingredients for reducing blood fat in the coral grass, acanthopanax senticosus and yam can be separately extracted into the coral grass extract, the acanthopanax extract and the yam extract.

In the concentration step S2, the coral grass extract, the acanthopanax extract and the yam extract are respectively concentrated to obtain a coral grass extract, a acanthopanax extract and a yam extract. For example, the concentration step S2 can be selected by concentration under reduced pressure, evaporation or heat drying, but not limited thereto. In this embodiment, the coral grass extract, the acanthopanax extract and the extract solvent of the yam extract are removed by concentration under reduced pressure to obtain the coral grass extract, the acanthopanax extract and the yam extract, respectively. Thereby, the effect of the coral grass extract, the acanthopanax extract and the hypolipidemic active ingredient in the yam extract can be improved.

In the mixing step S3, the coral grass extract, the acanthopanax extract and the yam extract are mixed into a Chinese herbal medicine extract mixture, wherein the coral grass extract accounts for 33.4 to 77.7% by weight of the Chinese herbal medicine extract mixture. The extract of Acanthopanax senticosus is 11.14~33.3% and the extract of the yam is 11.14~33.3%. More specifically, when the weight percentage of the coral grass extract is 71.4%, the weight percentage of the extract of Acanthopanax senticosus and the extract of yam is 14.3%, respectively, and there is no mutual inhibition between the extracts of the Chinese herbal medicines. Moreover, each of the Chinese herbal medicine extracts can produce a better synergistic effect, and the effect of stabilizing blood lipids and inhibiting fat accumulation can be exerted. Thus, the Chinese herbal medicine extract mixture for lowering blood fat of the present invention can reduce the effect of TC, TG or LDL-C in blood, and inhibit the accumulation of lipids in fat cells, thereby achieving the effect of reducing the risk of cardiovascular diseases in the brain.

The Chinese herbal medicine extract mixture for reducing blood fat of the present invention may further form the Chinese herbal medicine extract mixture into a pharmaceutical composition, preferably an oral dosage form, such as a lozenge, by a pharmaceutically acceptable carrier or excipient. Capsules, powders, granules or liquid preparations; or the Chinese herbal medicine extract mixture for reducing blood fat of the present invention is combined with other foods or beverages, and is administered orally to the living body in an edible form.

In order to confirm that the Chinese herbal medicine extract mixture for reducing blood fat of the present invention has the functions of stabilizing blood lipid parameters and inhibiting accumulation of lipids by fat cells, the following tests are prepared according to the above method for preparing Chinese herbal extracts containing different proportions of coral grass, acanthopanax senticosus and yam. To illustrate the effect of the herbal extract mixture of the present invention on stabilizing blood TC, TG, high-density lipoprotein cholesterol (HDL-C) and LDL, and the ratio of Chinese herbal medicine extract mixture for inhibiting fat cells. The role of lipid accumulation.

(A) Fat cell accumulation lipid test

In this example, 3T3-L1 fat cells (purchased from the Taiwan Hsinchu Food Industry Development Research Institute, BCRC 60159) were used as test platforms, and the ratios of different Chinese herbal extract mixtures and different concentrations of herbal extracts were observed. The inhibition of lipid accumulation in each group of cells.

More specifically, this example provides 11 sets of 3T3-L1 adipocytes (the number of cells in each group is 1×10 ⁴ cells/ml), and co-cultured for 24 hours at a temperature of 37 ° C and 5% carbon dioxide gas. Then, the co-cultures as shown in Table 1 (500 μg of the herbal extract mixture per ml of the culture solution) were added for 24 hours, and the lipid accumulation of each group of cells was observed by oil red staining, and the cells of each group were observed. The lipid accumulation was quantitatively determined, wherein the extraction solvent (water) was added as the control group in the A1-1 group, and no Chinese herbal extract mixture was added.

Comparing the 2a to 2k maps, the fat cells of the A1-1 group (Fig. 2a) were not co-cultured with any Chinese herbal extract mixture, and the area of the red staining of the fat cells of the A1-1 group was significantly larger than that of the other groups. The A1-2 to A1-4 groups were each added with 500 μg/ml of Coral Grass Extract (2b). Figure), 500μg/ml of Acanthopanax senticosus extract (Fig. 2c) or 500μg/ml of yam extract (Fig. 2d), although the amount of lipid in the fat cells is lower than that of the A1-1 group, The lipid content was still higher than the A1-5 to A1-11 groups.

Please refer to Figure 3 for the lipid quantitative bar graph of each group of fat cells, based on group A1-1 (percent lipid percentage 100%), wherein the fat of group A1-5 to group A-11 The lipid content in the cells was significantly lower than in the A1-1 to A1-4 groups. It can be seen that the herb extracting mixture for reducing blood fat in the present invention can effectively reduce the accumulation of lipids in fat cells by the synergistic action of coral grass, acanthopanax senticosus and yam extract.

In this embodiment, another group of 8T3-L1 fat cells (the number of cells in each group is 1×10 ⁴ cells/ml) is co-cultured for 24 hours at a temperature of 37 ° C and 5% carbon dioxide gas, and then added as After culturing the contents of different concentrations shown in Table 2 for 24 hours, the lipid accumulation of each group of cells was observed by oil red staining, and the lipid accumulation of each group of cells was quantified, wherein the extract of the A2-1 group was added. The solvent (water) was used as a control group, and no Chinese herbal extract mixture was added.

Please refer to Figure 4 for the lipid quantitative bar graph of each group of fat cells. Adding 100~700μg/ml herbal extract mixture can reduce the accumulated lipid content in fat cells.

Referring to Figures 5a to 5f and Figure 6, in this example, the fat cells of groups A2-1 and A2-6 were observed by oil red staining on days 3, 6 and 8 of the co-cultures. The accumulation of lipids in the fat cells.

As can be seen from Figures 5a to 5f and Figure 6, the present invention can achieve the effect of reducing the accumulation of lipids in fat cells by the interaction between coral grass extract, acanthopanax extract and yam extract, and The weight ratio of the Chinese herbal medicine extract mixture is 5:1:1, which is a coral seed extract, an extract of Acanthopanax senticosus and a yam extract, and the effect of reducing lipid accumulation in fat cells is better.

(B) High blood lipid animal model test

This example was tested in a Golden Syrian male hamster purchased from the National Laboratory Animal Breeding and Research Center, which was maintained at a temperature (25 ± 1 ° C) and illumination (12 hours of sunshine) maintained in a normal range. Allow hamsters to eat and drink freely, until four weeks old, start feeding high-fat diet (TestDiet® 5TJT, which contains 1% cholesterol), after 8 weeks of feeding (i.e., 12 weeks old), as a high-fat diet-induced hamster (HFD hamster) of the present example, was tested (B). In addition, in the test (B) of the present example, a group of normal hamsters were additionally provided as a control group (Group B0), and the feeding process was ingested until the age of 12 weeks.

"Feeding high-fat animals with different extracts"

In the present example, four groups of HFD hamsters of 12 weeks old (8 replicates in each group) were obtained in the above manner, and fed for 8 weeks under the feeding conditions as shown in Table 3, and their body weight changes were recorded. More specifically, the foods as shown in Table 3 were fed daily by the administration tube, wherein the group B1 was fed only with water; the HFD hamsters of groups B2, B3 and B4 were fed different doses respectively ( 1.5, 3.0 and 6.0 g / kg body weight / day) Chinese herbal medicine extract mixture, wherein the herbal extract mixture in the present embodiment comprises coral grass extract, acanthopanax extract and yam extract in a weight ratio of 5:1:1. Things.

Please refer to the fourth table, which is the blood lipid parameters of the groups B0 to B4 of the present embodiment after 8 weeks, respectively. The blood lipid parameters are triglyceride (TG), total cholesterol (TC), and high-density lipoprotein cholesterol. (HDL-C) and low density lipoprotein cholesterol (LDL-C).

As can be seen from the fourth table, the TC, TG, and LDL-C values of the B2 to B4 groups decreased after 8 weeks, compared with the B1 group, and the body weight of the B2 to B4 groups was compared with the B1 group. Significant differences ( p < 0.05), and the lipid parameters were positively correlated with the concentration of the Chinese herbal extract mixture. The intake of the experimental hamster ingestion of the Chinese herbal extract mixture was converted to the recommended daily intake of 60 kg of adults, which was 150-600 mg. It can be seen that the concentration of the herbal extract mixture of the present invention is 1.5 to 6.0 g/kg/day, which has the effect of lowering the blood lipid parameters and the weight of the experimental hamster.

In summary, the Chinese herbal medicine extract mixture for reducing blood fat of the present invention combines the activities of three different natural plants - coral grass, acanthopanax senticosus and yam. Ingredients, the three Chinese herbal extracts inhibit lipid accumulation in fat cells, and help to inhibit blood lipid parameters (such as TG, TC, and LDL-C) in hyperlipidemia animals, and the specific ratio combinations of the Chinese herbal extract mixtures are not only not mutually Inhibition, on the contrary, has synergistic effects and adds the effects of reducing blood lipids.

In summary, an effective dosage (e.g., 150 to 600 mg) of the herbal extract mixture of the present invention is combined with at least one pharmaceutically acceptable carrier or excipient to form a pharmaceutical composition, the herbal extract mixture It is prepared by the method as shown in Fig. 1. It is preferred to administer 150-600 mg/kg body weight per unit body weight, and the administration time can last for 8 weeks to achieve stable blood lipid parameters. And control the effect of fat cells in the body to accumulate fat. In addition, the main active ingredient of the pharmaceutical composition for reducing blood fat of the present invention is derived from natural herbs - coral grass, acanthopanax senticosus and yam, so that the organism is mild and non-toxic, and the user does not need to worry about the The pharmaceutical composition has hepatotoxicity and can increase the user's willingness to take the pharmaceutical composition.

Thereby, the Chinese herbal medicine extract mixture for reducing blood fat of the present invention achieves the effect of effectively reducing the risk of cardiovascular and cardiovascular diseases by stabilizing blood lipid parameters and inhibiting accumulation of lipids by fat cells.

The Chinese herbal medicine extracting mixture for reducing blood fat of the invention comprises the extracts of coral grass, acanthopanax senticosus and yam, which has the effect of inhibiting the accumulation of lipids of fat cells to achieve the effect of effectively lowering blood fat.

The pharmaceutical composition for lowering blood fat of the present invention comprises an extract of coral grass, acanthopanax senticosus and yam, which can synergistically effect each other to achieve an effect of effectively improving blood fat lowering effect.

Although the present invention has been disclosed using the above preferred embodiments, it is not In order to limit the scope of the present invention, it is still within the spirit and scope of the present invention that various modifications and changes to the above-described embodiments are still within the technical scope of the present invention. The scope defined in the patent application is subject to change.

S1‧‧‧ extraction step

S2‧‧‧ Concentration step

S3‧‧‧ mixing step

Fig. 1 is a block diagram showing the preparation steps of the coral grass extract, the acanthopanax extract and the yam extract in the Chinese herbal medicine extract mixture for reducing blood fat.

Figure 2a: Oil red staining of fatty cells in group A1-1 of this example.

Figure 2b: Oil red staining of fat cells in Group A1-2 of this example.

Figure 2c: Oil red staining of the fat cells of Group A1-3 of this example.

Figure 2d: Oil red staining of fat cells of Group A1-4 of this example.

Figure 2e: Photograph of the fat red staining of the fat cells of Group A1-5 of this example.

Figure 2f: Oil red staining of fat cells of Group A1-6 of this example.

Figure 2g: Oil red staining of fat cells of Group A1-7 of this example.

Figure 2h: Oil red staining of fat cells of Group A1-8 of this example.

Figure 2i: Oil red staining of fat cells of Group A1-9 of this example.

Fig. 2j: Photograph of oil red staining of fat cells in the group A1-10 of this example.

Figure 2k: Oil red staining of fat cells of Group A1-11 of this example.

Fig. 3 is a bar graph showing the relative percentage of lipid accumulation in the groups A1-1 to A1-11 of the present Example.

Fig. 4 is a bar graph showing the relative percentage of lipid accumulation in the groups A2-1 to A2-8 of the present Example.

Fig. 5a: Photograph of oil red staining of fat cells after 3 days of culture in the A2-1 group of the present Example.

Figure 5b: Photograph of oil red staining of fat cells after 6 days of culture in Group A2-1 of this example.

Fig. 5c: Photograph of oil red staining of fat cells after 8 days of culture in the A2-1 group of the present Example.

Figure 5d: Oil red staining of fat cells after 3 days of culture in Group A2-5 of this example.

Figure 5e: Oil red staining of fat cells after 6 days of culture in Group A2-5 of this example.

Fig. 5f is a photograph of oil red staining of fat cells after 8 days of culture in Group A2-5 of the present Example.

Fig. 6 is a bar graph of the absorbance OD _{500 of the} groups A2-1 and A2-6 of the present embodiment after different culture days.

S1‧‧‧ extraction step

S2‧‧‧ Concentration step

S3‧‧‧ mixing step

Claims (9)

A Chinese herbal medicine extract mixture for lowering blood fat, comprising: a coralweed extract of 33.4 to 77.7% by weight; a scutellaria extract of 11.14 to 33.3% by weight; and a weight percentage of 11.14 to 33.3% Yam extract; wherein the coral grass extract, the acanthopanax extract and the yam extract are obtained by the following steps: an extraction step using water as an extraction solvent, respectively, on coral grass samples, thorns Five samples and yam samples were extracted to obtain a coral grass extract, a acanthopanax extract and a yam extract, wherein the extraction solvent and the coral grass sample, the acanthopanax sample and the yam sample respectively The weight ratio is 1:8~1:12; in a concentration step, the coral grass extract, the acanthopanax extract and the yam extract are respectively concentrated to obtain a coral grass extract and a thorn Adding the extract and a yam extract; and mixing the coral grass extract, the acanthopanax extract and the yam extract into the Chinese herbal medicine extract mixture; wherein the extracting step Shan Hucao whole plant parts of the sample line comprises Shan Hucao Acanthopanax the sample extraction system comprising the steps of Acanthopanax root or stem, and the yam sample extraction system comprising the steps of earthnuts yam. The Chinese herbal medicine extract mixture for lowering blood fat as described in claim 1, wherein the coral grass sample, the acanthopanax sample and the yam sample are fresh, dried or processed products which are not dried or exposed. Chinese herbal medicine extract for lowering blood fat as described in item 2 of the patent application scope The mixture was taken, and the coral grass sample was freeze-dried to obtain a dried coral grass. The Chinese herbal medicine extract mixture for lowering blood fat as described in claim 2, wherein the yam sample is dried by heat to obtain a dried yam. The Chinese herbal medicine extract mixture for lowering blood fat as described in claim 1, wherein the temperature of the extraction step is 40 to 60 °C. The Chinese herbal medicine extract mixture for lowering blood fat as described in claim 1, wherein the extraction step is extraction for 6 to 10 hours. A pharmaceutical composition for lowering blood fat, comprising: a herbal extract mixture as described in claim 1; and a pharmaceutically acceptable carrier or excipient to form the herbal extract mixture into the medicine combination. The pharmaceutical composition for lowering blood fat as described in claim 7 wherein the dosage of the Chinese herbal medicine extract mixture is 150-600 mg. A pharmaceutical composition for lowering blood fat as described in claim 7, wherein the pharmaceutical composition is in the form of a tablet, a capsule, a powder, a granule or a liquid.