KR101135957B1 - Concentrated extract from herb medicines, and healthy food and pharmaceutical composition for prevention and treatment of diabetic mellitus, pancreatitis, nephritis and diabetic nephropathy - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure, and a dietary supplement containing the yellow lotus, powder and pig pancreas and a method for producing the same.

According to the present invention, diabetes mellitus, pancreatitis, nephritis and diabetic renal failure may be reduced by reducing hyperglycemia due to diabetes and protecting the pancreas and kidneys from tissue damage caused by hyperglycemia, inflammation, and blood circulation disorders, which are causes of diabetes and diabetic complications. Prophylactic and therapeutic effects can be maximized, and side effects can be provided pharmaceutical compositions and dietary supplements.

Husband, acid, pig pancreas, diabetes mellitus, pancreatitis, nephritis, diabetic renal failure

Description

Extracted concentrates obtained from herbal medicines and health foods and pharmaceutical compositions for the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure PANCREATITIS, NEPHRITIS AND DIABETIC NEPHROPATHY}

The present invention relates to a composition for the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure, and more particularly to a pharmaceutical composition and dietary supplement containing a sulfur, acid and pig pancreas and a method for producing the same.

Diabetes mellitus is a metabolic disease characterized by hyperglycemia caused by insulin secretion or a deficiency of insulin action. It is caused by a lack of insulin or a decrease in the biological effects of insulin on target cells, leading to long-term complications such as kidney failure. It is a chronic degenerative disease accompanying. Diabetes causes various complications from the onset of the onset, including acute complications such as hypoglycemia, ketoacidosis, diabetic coma, and local systemic bacterial infections, diabetic nephropathy, hypertension, diabetic retinopathy and cataracts, neuropathy, and cardiovascular complications. It is accompanied by chronic complications.

Currently, the prevalence of diabetes has increased year by year due to the rapid change of the diet, which is about 5-10% in Korea. According to 2005 statistics, the number of diabetics exceeded 4 million, 8.3 out of 100, and 2025. It is estimated that the number will increase to about 6.8 million by year (Korea Diabetes Information Center, 2005). Diabetes is the third most serious disease in the world, the fourth most common cause of death in Korea, and its lifespan is reduced by 5-10 years due to various complications. Diabetes mellitus is a metabolic syndrome characterized by hyperglycemia caused by a deficiency in insulin action, insulin secretion, or both, resulting in chronic chronic hyperglycemia as well as lipid and protein metabolism, resulting in retinal, kidney, nerve, It causes complications such as cardiovascular system, which causes pain and fatal problems of lifespan. In recent years, while the number of diabetics is increasing, the importance of developing a cure for diabetes is emphasized in a situation in which continuous and proper treatment is difficult.

Diabetes treatment is roughly divided into diet, exercise therapy and drug therapy, and it is determined by the patient's condition. Diabetes treatment is almost impossible to cure after the onset, and the purpose of the secondary prevention is to improve symptoms and prevent acute complications. have. In other words, diabetes is more important than the disease itself, so prevention and treatment of complications is very important to maintain the blood sugar and prevent complications. Current antidiabetic agents include oral hypoglycemic agents such as sulfonate agents, biguanides, alpha-glucosidase inhibitors, thiazolidinediones, and the like. Insulin therapy, pancreas transplantation, herbal therapy, and alternative medicine are used. Diabetes mellitus is caused by a variety of pathogens, so the treatment also has to vary, and moreover, many classic treatments do not see satisfactory effects, so new treatments are required. Currently, many studies have been conducted on some insulin promoters, insulin potentiators, insulin resistance enhancers, drugs that exhibit insulin-like effects in target tissues, staphylosynthesis inhibitors, insulin antagonist inhibitors, drugs that delay carbohydrate absorption, and amylin analogs. However, many of them are still in insufficient stages to be used in the human body or have side effects due to toxicity.

On the other hand, the limitation of Western medicine is that it is best to find out the root cause of the disease and not treat the symptoms. Therefore, Western medicine that only treats the result of the disease can increase the drug dependency of the patients. On the other hand, in Korean medicine, diabetes is called thirst, which is caused by excessive stress and nerve hypersensitivity, and is caused by overeating, drinking, excessive intake of fat, lack of kidney soundness, and lack of exercise. It is considered to be caused by heat radiation. In Chinese medicine, the cause of diabetes is regarded as crude, fever, and fire, and if the disease progresses for a long time, it replenishes the essence, water, and um, and eliminates fever. Treatment should be performed, depending on the patient's constitution or pathogenesis, symptoms will be appropriately treated. In addition, depending on the symptoms, Sogal is classified into upper (small), small (medium), calcined (下 消), and the symptoms are different. The small appeal is Daum, small and medium meals, and calcined multi urine ( It appears as a phenomena, and the prescription varies depending on the symptoms, such as Cheongshimyeon consonant (상 心 連 字 飮) in small and medium, Sangjipalmultang in small and medium size, and Shingihwa (하) in calcination. Use Thus, traditional Chinese medicine preparations have been used to treat human diseases and protect the body for a long time, and most of them are plant origins, mainly based on the compound prescription of herbal medicines, and are based on the prescription of herbal medicines such as Dongbobom. These herbal medicines are used as a preventive and therapeutic agent for various diseases, and the effects are compounded and the side effects are less and the interest is amplified due to the chemical side effects of many synthetic drugs that are currently developed from a bilateral perspective.

Sodanghwan (消 糖丸) is a prescription consisting of two Chinese herbs and the pancreas of pigs, one of the constituents of the medicinal herb (黄 련; Coptidis Rhizoma, Coptis japonica Makino) is a perennial plant belonging to the Ranunculaceae family It is native to medicinal herbs and is cultivated in Korea, Japan, China, etc., and in oriental medicine, it uses rhizome, and it is high, cold, non-toxic and deep. (心), liver (肝), stomach (胃), large intestine (大腸) and acts, such as blue-heat damp bath, cheongsimjebun (淸 心 除煩), detoxification (瀉火 解毒) efficacy Moist heat obesity, guto carbonic acid, saree, jaundice, high heat honeymoon, deepening star, heart fire It is used to treat diseases such as the heart, blood fever soil, the purpose, pain, sogal, and carbuncle. ¹⁾ Known. There are many experimental studies on sulfur, especially the strong antibacterial effect of berberin, anti-inflammatory, hemostasis, lowering blood pressure, anticancer activity, etc., and it has central nervous system suppression, nephritis treatment, bronchial smooth muscle dilation and hypoglycemic effect. ^2-4) .

One of the medicinal herbs of Sodanghwan is the medicinal herb of Dioscorea batatas Decne and D. japonica Thunb. It is a removed rhizome, distributed and cultivated all over Korea. Pesticides are flat, persimmon, secretory, pulmonary, nervous, etc. Insertion (補腎 澁 精). Chibi Hearing Culinary School, Old Buddha, Old Ruins, Sludge Hearing Well, Daeha, Urinary Bin, Heyeol Soup Efficacy of and so on. Basically, it is applied to the effect of flatness when the yangyang is weak or the nasal sound is weak due to the action of the bowel. In addition, it is effective in supplementing and converging pulmonary sinus, and it is highly effective in the insertion of dementia. (腎虛 遺精), lobster (, 下), urine bin (尿頻), sogal (消渴) ^{5) It is applied} . Experimental studies on caustic drugs reported hypoglycemic effect, prevention of NGF-induced neuropathy, gastric juice secretion effect, immune disease reduction, anti-inflammatory effect, tumor growth inhibition effect and osteoporosis effect in diabetic animal model ^{6 -9)} . The pancreas of pigs has been used in the private sector to overcome menopausal disorders and improve diabetes by eating pills with herbs in powder.

Reference

1. Compiled by the National Institute of Oriental Medicine for Compilation of Textbooks. Herbology. Younglim Party. 2007. Chapter 2; 218 to 200.

2.Yin J, Zhang H, Ye J. Traditional chinese medicine in treatment of metabolic syndrome. Endocr Metab Immune Disord Drug Targets. 2008; 8: 99-111.Review.

Tang LQ, Wei W, Chen LM, Liu S. Effects of berberine on diabetes induced by alloxan and a high-fat / high-cholesterol diet in rats. J Ethnopharmacol. 2006; 108: 109-15.

Liu WH, Hei ZQ, Nie H, Tang FT, Huang HQ, Li XJ, Deng YH, Chen SR, Guo FF, Huang WG, Chen FY, Liu PQ. Berberine ameliorates renal injury in streptozotocin-induced diabetic rats by suppression of both oxidative stress and aldose reductase. Chin Med J. 2008; 121: 706-12

5. Compiled Committee for Compilation of Teaching Materials for Korean Medicine. Herbology. Younglim Party. 2007. Chapter 17; 581-582.

6. Kang Dong-ho, Choi Sang-jin, Lee Tae-ho, Son Mi-won, Park Ji-ho, Kim Sun-yeo. Study on antidiabetic properties of powder (1). Korean Journal of Food and Nutrition. 2008; 21: 425-429.

7. Jeon Jeong Rye, Lee Ji Sun, Lee Chu Hee, Kim Jong Yeon, Kim Soon Dong, Nam Doo Hyun. Effect of Dioscin-Containing Ethanol Extracts on Gastrointestinal Function in Rat Model. Arch Pharm Res. 2006; 29: 348-353.

Zhao G, Kan J, Li A, Chen Z. Structural features and immunological activity of a polysaccharide from Dioscorea opposita Thunb roots. Carbohyd. Polumers. 2005; 61: 125-131.

9.Kim MJ, Kim HN, Kang KS, Baek NI, Kim DK, Kim YS, Jeon BH, Kim SH. Methanol extract of Dioscoreae Rhizoma inhibits pro-inflammatory cytokines and mediators in the synoviocytes of rheumatoid arthritis. Int Immunopharmacol. 2004; 4: 1489-97.

Accordingly, the present inventors have made intensive studies to develop a preventive and therapeutic agent for diabetes, pancreatitis, nephritis and diabetic renal failure using herbal medicines with low side effects. Effects, as well as increased insulin secretion, reduced triglyceride accumulation, reduced oral glucose load, decreased blood urea and reduced creatinine in blood, thus providing excellent effects in the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure. Confirmed that the present invention was completed.

Therefore, the main object of the present invention is to provide a pharmaceutical composition and health functional food and a method for producing the same, which have fewer side effects, and have an excellent effect in the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure. have.

According to one aspect of the present invention, the present invention provides a pharmaceutical composition for preventing and treating diabetes mellitus, pancreatitis, nephritis and diabetic renal failure, containing nasturtium, powder and pig pancreas.

In the composition of the present invention, it is preferable that the ratio of the above-mentioned yellow lotus, powder and pig pancreas is 1: 1: 1 by weight.

According to another aspect of the present invention, the present invention provides a dietary supplement for preventing and improving diabetes, pancreatitis, nephritis and diabetic renal failure comprising the composition.

According to another aspect of the present invention, the present invention provides a method for preparing a pharmaceutical composition for the prevention and treatment of diabetes mellitus, pancreatitis, nephritis and diabetic renal failure, characterized in that the mixture of sulfur, acid and pig pancreas is finely prepared and pill. to provide.

According to another aspect of the present invention, the present invention comprises the steps of preparing a powder by grinding a mixture of nasturtium, powder and pig pancreas; Eluting by adding water or ethanol to the powder; It provides a method for producing a pharmaceutical composition for preventing and treating diabetes mellitus, pancreatitis, nephritis and diabetic renal failure comprising the step of filtering and concentrating the eluate.

In the composition production method of the present invention, it is preferable to add the water or ethanol 10 to 30 times the weight of the powder.

In the composition production method of the present invention, it is preferable to use a vacuum concentration method as the concentration method.

In the composition preparation method of the present invention, after the step of filtering and concentrating the eluate, it is preferable to further comprise the step of freeze drying.

Hereinafter, the present invention will be described in more detail step by step.

As used in the present invention, Hunan (Coptidis Rhizoma, Coptis japonica Makino) is a perennial herbaceous plant belonging to Ranunculus (Ranunculaceae), which is native to China and is grown in Korea, Japan, China, etc. It is good to use rhizome (根莖).

Mountain medicinal plant is a rhizome removed from the pericarp of Dioscorea batatas Decne and D. japonica Thunb. It is distributed and cultivated everywhere.

The composition of the present invention includes the sulfur and acid, as well as the pig pancreas, wherein the pig pancreas is preferably dried.

When preparing the composition of the present invention, it is preferable to mix the sulfur, acid and pig pancreas in a weight ratio of 1: 1: 0.2, and the mixture can be prepared in the form of a pill or extract by granulation. have.

When the extract is prepared, it is better to elute by adding water or ethanol as a solvent to the powder obtained by grinding the mixture. Of these, it is preferable to use water, and water or ethanol is 10 to 30 times based on the weight of the powder. More preferably, 20 times is added. If too little of the solvent is added, it is difficult to sufficiently extract the active ingredients of the sulfuric acid, the caustic agent and the pig pancreas, and if it is added too much, it is good to extract the active ingredient sufficiently, but it is appropriate when the filtration or vacuum concentration method is used. Because you can not.

When preparing an extract, it is preferable to add a solvent to the mixture powder, and then elute by heating in a shaker. It is preferable to filter the eluted eluate, in which case it is most preferable to use two-ply gauze or Whatman filter paper.

When water is used as a solvent, the extract may be used as it is, but it is preferable to use ethanol as a solvent or to concentrate to increase the efficacy of the extract. In addition, it is good to use the freeze-dried and stored at -20 ℃ for storage and convenience of use.

The composition of the present invention may be administered in a conventional formulation, and administration by oral administration is preferred because the effect is fast and side effects are avoided. Such conventional oral dosage forms and injectable forms may contain various excipients, carriers, diluents, and the like, which can be readily selected by those skilled in the art.

In addition, the effective amount of the composition can be determined within a wide range depending on the route of administration, the dosage form, the purpose of use, the degree of disease of the patient, and the like. In general, however, administration at a concentration of 30 to 500 mg / kg / day is effective in terms of the prevention and treatment effects of diabetes, based on the results proved by the present inventors.

In order to confirm the efficacy of the composition, the present inventors administered the composition to the normal group and the diabetic inducing group in animals (rats), and then the weight, blood sugar, insulin secretion, triglyceride in blood, blood urea, blood creatinine and oral sugar The change in load capacity was investigated.

Diabetes induces not only a rapid weight loss, but also a rapid increase in the concentration of glucose in the blood, a breakdown of the beta cells of the pancreas, a decrease in insulin secretion, an increase in triglyceride accumulation, an increase in urea concentration, Symptoms of creatinine accumulation

When the above symptoms are normalized by administration of the composition, which means that there is an effect on diabetes, by experimenting the composition of the present invention by protecting the pancreas and kidneys from tissue damage caused by hyperglycemia, inflammation, blood circulation disorders, etc. It has been shown to be effective in diabetes, pancreatitis, nephritis and diabetic renal failure.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Preparation of the Composition of the Invention

The medicinal herbs used in this experiment were purchased from Gwangmyeongdang Pharmaceutical Co., Ltd. (Ulsan, Korea) and tested and selected at the Department of Herbology, College of Oriental Medicine, Dongguk University.

Based on the weight, it was quantified and finely mixed so as to have a blending ratio of 1, 4, 1, and 0.2 of dried pig pancreas, and then a powder (sodan-sugar, SDH) and extract (so-tang-tang, SDT) were prepared using the powder. After adding 20 times the volume of distilled water based on the weight of the powder to the powder, eluted for 3 hours using a gas bath, it was filtered through two layers of gauze, concentrated under reduced pressure with a rotary evaporator and lyophilized. Prepared. The total yield of the extract was 34.8%, and the extract extracted immediately before the animal experiment was dissolved in physiological saline while being stored at low temperature.

Experimental Example 1. Effect of the present invention composition

In the present experimental example, the effect of the composition of the present invention was confirmed in the rats, and divided into the untreated group (normal group) and the diabetic induction group, and the diabetic induction group was treated with physiological saline treatment group, sodanghwan treatment group, and Sodangtang, respectively. Experiments were carried out divided into treatment group and glybenclamide (a type of diabetes treatment) treatment group.

end. Purchase and breeding of experimental animals

Male Sprague-Dawley rats (8 weeks old, 250-300 g) were purchased from Central Experimental Animal Co., Ltd. and adapted to laboratory environment (temperature 22 ± 3 ℃, humidity 50 ± 10 ℃) for about a week. After use it was used. Three to four rats were placed in each cage, and water and feed (solid feed: experimental rats, central experimental animals) were allowed to freely feed.

I. Diabetes animal model production

Streptozotocin (STZ) was dissolved in 0.01 M citrate buffer (pH 4.5) to a concentration of 45 mg / kg, and then intraperitoneally administered 0.1 ml per 100 g of rat body weight. After 72 hours of STZ injection and fasting for 12 hours, blood was collected from the tail, and glucose levels were measured and only rats with a concentration of 300 mg / dL or more were used for the experiment.

All. Drug administration in animal models of diabetes

Each experimental group composed of diabetic animal models adjusted the mean value of blood glucose levels between groups, and each group was treated with physiological saline, Sodanghwan (60 mg / kg), Sodangtang (100 mg / kg), and glibenclamide (1-3 mg / kg). Kg) was administered orally at 0.1 ml per 100 g body weight for 4 weeks. At this time, saline and sodanghwan (30mg / ㎏) was administered once a day for four weeks, sodangtang (100mg / ㎏) and glybenclamide was administered twice a day.

la. Weight measurement and blood collection

During the experiment, the weight change of the rat experimental group was measured every week, and the weight on day 0 was administered as the initial weight and the weight at 4 weeks as the final weight.

Once every week, all rats were fasted for 12 hours, and blood was collected from the tail vein, and serum was separated by centrifugation at 5,000 rpm for 10 minutes. The separated serum was stored at -20 ℃ and used as a sample for serum analysis. On the last autopsy day, Rompun? 0.2 mg / kg was injected into the abdominal cavity to sed, and Ketalar? 1 mg / kg was injected into the abdominal cavity for anesthesia, followed by an open abdominal blood collection. The collected blood was centrifuged within 1 hour to separate serum.

hemp. Effect on weight loss

As a result of observing the weight change of the experimental group of rats at 0, 2, and 4 weeks, as shown in FIG. 1, normal rats (Saline-C) administered with saline showed body weight at 4 weeks compared to the initial weight (276.3 ± 12.56 g). (338.7 ± 7.50g) increased normally, whereas STZ-induced diabetic rats (STZ-C) significantly lost weight at 4 weeks compared to their initial weight (273.3 ± 14.53g) (182.5 ± 41.33g). Sodanghwan group (SDH-60, 237.5 ± 8.66g), sodangtang group (SDT-100, 236 ± 15.3g) and glybenclamide group (Gliben-1, 213.7 ± 22.12g) had significant weight in diabetic rats. Increased ( p <0.05). Therefore, both Sodanghwan and Sodangtang have been shown to suppress weight loss in diabetic rats.

bar. Effects on Blood Sugar Changes

An enzyme reagent (Asan Pharmaceutical, Seoul) using a glucose oxidase reaction was used, and blood glucose was measured by colorimetric determination at 500 nm.

As a result, as shown in FIG. 2, the blood glucose of normal saline-administered rats (Saline-C) was measured at a normal range of 116.6 ± 51.98 mg / dL, and diabetic rats (STZ-C) were 388.01.3 ± 23.19. It was measured as high as mg / dl. On the other hand, in the group treated with Sodanghwan (SDH-60) and Sodang-tang (SDT-100), it was measured to be 291.0 ± 72.84 mg / dL and 203.7 ± 14.9 mg / dL, respectively ( p <0.01), resulting in a significant decrease in blood glucose levels. Appeared. On the other hand, even in the glybenclamide administration group (G-3) was measured to 152.8 ± 26.56 mg / ㎗ appeared to significantly reduce the blood sugar level. Therefore, both Sodanghwan and Sodangtang have been shown to improve diabetes by controlling hyperglycemia in diabetic rats.

four. Effect on insulin secretion

Rat insulin ELISA kit (Central Laboratory Animal, Japan) was used and insulin concentration was measured by measuring absorbance at 450 nm.

As shown in FIG. 3, the normal control group (Saline-C) administered with saline was measured to 0.43 ± 0.019 mg / dL, while the diabetic rat (STZ-C) decreased to 0.26 ± 0.120mg / dL. It was. Meanwhile, the Sodanghwan-administered group (SDH-60) was measured to be 0.27 ± 0.021 mg / dL, and the Sodangtang-administered group (SDT-100) was measured to be 0.36 ± 0.03mg / dL, and the glybenclamide-treated group (G-1) was 0.479 ±. Measured at 0.014 mg / dl, insulin concentrations were significantly increased in Sodanghwan, Sodangtang, and Glibenclamide treated groups. Thus, Sodanghwan and Sodangtang have been shown to improve insulin secretion as well as glybenclamide.

Ah. Effect on triglycerides

The concentration of tryglyceride in serum was measured using TG-S kit (Asan Pharmaceutical, Seoul) as a kinetic alkaline picrate method.

As a result, as shown in Figure 4, the control group (Saline-C) administered with saline was measured as 116.6 ± 51.9 mg / ,, diabetic rats (STZ-C) was increased to 234.9 ± 20.88 mg / ㎗. Meanwhile, 165.1 ± 32.86mg / dl and 199.4 ± 13.22mg / dl in sodanghwan (SDH-60) and sodangtang (SDT-100), respectively, and 235.3 ± 27.43mg / dl in glybenclamide treated group (G-1). Was measured. Therefore, administration of Sodanghwan and Sodangtang significantly inhibited the increase of triglyceride in diabetic rats.

character. Effect on elements in the blood

Using ^{Quanti Chrom TM Urea Assay Kit (BioAssay} Systems, USA) to measure the blood urea (BUN) concentration as a colorimetric assay method.

As shown in FIG. 5, the concentration of serum BUN was measured to 40.53 ± 5.13 mg / dL in saline-C, and diabetic rats (STZ-C) were measured to be 115.7 ± 13.86mg / dL. It became. In addition, the concentrations of BUN in the case of Sodanghwan (SDH-60) and Sodangtang (SDT-100) were 128.4 ± 16.12mg / dL and 131.7 ± 7.91mg / dL, and glybenclamide (G-1). In 126.2 ± 17.16 ㎎ / ㎗ did not decrease the concentration of BUN. In other words, the administration of Sodanghwan, Sodangtang and Glibenclamide did not appear to affect the accumulation of BUN due to diabetes complications.

car. Effects on creatinine in the blood

Creatinine Assay Kit (BioAssay Systems) was used to measure the concentration of creatinine in the blood by colorimetric analysis.

As a result, as shown in Figure 6, the concentration of serum creatinine was measured to 0.42 ± 0.02 mg / 에서는 in the saline control group (Saline-C), diabetic rats (STZ-C) was measured as high as 1.62 ± 0.35 mg / ㎗ It became. In the case of administration of Sodanghwan (SDH-60), 0.53 ± 0.13mg / dL and Sodangtang (SDT-100) were 0.47 ± 0.12mg / dL ( p <0.01). The number decreased. On the other hand, the glybenclamide administration group (G-1) was 2.22 ± 0.37 mg / ㎗ did not appear to decrease the creatinine level. Therefore, administration of Sodanghwan and Sodangtang has been shown to reduce the accumulation of creatinine due to diabetes.

Ka. Effect on Oral Glucose Load Capacity

Oral glucose tolerance test is a commonly used method of diagnosing diabetes. It is a method to check high blood sugar by administering sugar into the body and collecting blood by measuring blood glucose levels. In humans, 2 hours after eating fasting glucose Diabetes is diagnosed when blood glucose does not fall to normal and is 200 mg / dl or more. Therefore, when the composition of the present invention is administered in the present experimental example, the effect on the oral glucose load capacity was confirmed.

STZ-induced diabetic rats were treated with saline and drugs for 4 weeks, and then fasted for at least 12 hours, and then 1 ml of sodanghwan and glybenclamide were administered. After 10 minutes, glucose (1 g / kg / mL) was orally administered, and blood was collected from the tail vein at 0, 30, 60, 90, and 120 minutes, serum was isolated, and the concentration of glucose in serum was measured. It was measured (oral glucose tolerance test, OGTT).

As a result, as shown in Figure 7, in the control group administered saline (Saline-C) 114 ± 7.4 mg / ㎗, 133.9 ± 16.7 mg / in order of 0 minutes, 30 minutes, 60 minutes, 90 minutes, 120 minutes ㎗, 139.2 ± 17.6mg / dl, 119.5 ± 11.5mg / dl, 119.01 ± 9.64mg / dl, and 464.2 ± 46.3mg / dl, 544.1 ± 66.4mg / dl, 549.2 ± in diabetic rats (STZ-C). 43.02 mg / dL, 536.1 ± 54.3 mg / dL, and 530.0 ± 45.2 mg / dL were measured to maintain hyperglycemia even after 120 minutes of glucose concentration in serum. Meanwhile, the Sodanghwan-administered group (SDH-60) was measured as 269.6 ± 98.6mg / dl, 390.0 ± 79.7mg / dl, 419.1 ± 55.6mg / dl, 402.01 ± 84.7mg / dl, and 392.2 ± 73.3mg / dl. The initial blood glucose level was decreased and significantly decreased ( p <0.001) after 120 minutes. Glybenclamide-treated group (G-1) also had an initial blood sugar reduction of 357.1 ± 23.1 mg / dL, 431.0 ± 40.2mg / dL, 429.1 ± 55.1mg / dL, 419.8 ± 50.7mg / dL, and 400.7 ± 57.7 mg / dL. After 120 minutes, blood glucose levels were significantly decreased ( p <0.001). Thus, the administration of Sodanghwan has been shown to significantly reduce the glucose tolerance increased by diabetes.

All the experimental results were expressed as mean ± standard deviation (SD) for three replicates. Statistical analysis was determined by Student t- test of the GraphPad program, and was found to be significant when the p value was 0.05 or less. .

As described above, according to the present invention, diabetes mellitus, pancreatitis, nephritis by reducing the hyperglycemia caused by diabetes and protecting the pancreas and kidneys from tissue damage caused by hyperglycemia, inflammation, and blood circulation disorders that are the causes of diabetes and diabetic complications And it can maximize the prevention and treatment effect of diabetic renal failure, can provide a pharmaceutical composition and health functional food with less side effects.

Figure 1 is a graph showing the measurement of the weight of each experimental group. [Saline-C, normal saline administration to normal rats; STZ-C, physiological saline administered to diabetic rats; SDH-60, Sodanghwan administration in diabetic rats (60 mg / kg); SDT-100, Sodang-tang administration in diabetic rats (100 mg / kg); Gliben-1, administration of glybenclamide to diabetic rats (1 mg / kg) as indicated in the drawings below]

Figure 2 is a graph showing the measurement of blood sugar in each experimental group. [G-3, administration of glybenclamide to diabetic rats (3 mg / kg)]

Figure 3 is a graph showing the measurement of insulin concentration in the blood of each experimental group. [G-1, administration of glybenclamide to diabetic rats (1mg / kg)]

Figure 4 is a graph showing the measurement of the concentration of triglycerides in the blood of each experimental group.

5 is a graph showing the measurement of the concentration of urea in the blood of each experimental group.

Figure 6 is a graph showing the measurement of creatinine concentration in the blood of each experimental group.

7 is a graph showing oral glucose tolerance measured in each experimental group.

Claims (8)

A pharmaceutical composition for preventing and treating diabetes mellitus, pancreatitis, nephritis and diabetic renal failure, which contains only nasturtium, powder, and pig pancreas as an active ingredient. A health functional food for preventing and improving diabetes, pancreatitis, nephritis and diabetic renal failure, which contains only nasturtium, powder and pig pancreas as active ingredients. The composition according to claim 1, wherein the ratio of the sulfur, acid and pork pancreas is 1: 1: 0.2 by weight. delete delete delete delete delete

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