CN101579421A - Chinese herbal medicinal health capsule for assisting in preventing and treating alcoholic liver injury, and preparation method thereof - Google Patents
Chinese herbal medicinal health capsule for assisting in preventing and treating alcoholic liver injury, and preparation method thereof Download PDFInfo
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- CN101579421A CN101579421A CNA2009100330566A CN200910033056A CN101579421A CN 101579421 A CN101579421 A CN 101579421A CN A2009100330566 A CNA2009100330566 A CN A2009100330566A CN 200910033056 A CN200910033056 A CN 200910033056A CN 101579421 A CN101579421 A CN 101579421A
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Abstract
The invention discloses a Chinese herbal medicinal health capsule for assisting in preventing and treating alcoholic liver injury, and a preparation method thereof. The capsule is filled with dry extract powder of kudzuvine roots, kudzuvine flowers, semen hoveniae, penthorum, hawthorn fruits and liquorice added with vitamin C, vitamin E and vitamin B1/B6. Toxicology tests show that the produce is safe and non-toxic. Health function tests show that the product can lower the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, increase the activity of enzymes clearing free radicals in liver tissue and significantly reduce or prevent alcoholic liver injury caused by excessive drinking.
Description
Technical field
The present invention relates to the health food technology field, be specifically related to herbal health care capsule of a kind of assisting in preventing and treating alcoholic liver injury and preparation method thereof.
Background technology
China is a Chinese liquor consumption big country.In recent years, China is in rising trend because of the sickness rate of the alcoholic liver injury due to the long-term excessive consumption of alcohol.Alcoholic liver injury is meant long-term heavy drinking or contains the liver disease that alcoholic acid beverage causes, and comprises light disease alcoholic liver disease, alcoholic fatty liver, alcoholic hepatitis, alcoholic fibrosis, alcoholic cirrhosis.Alcoholic liver injury has become the main hepatic injury reason that is only second to viral hepatitis.
Alcoholic acid oxidative metabolism mainly carries out in liver, and influences the oxidative metabolism balance of liver, and simultaneously, ethanol can form reactive oxygen free radical by the excited oxygen molecule.Long-term heavy drinking can produce a large amount of reactive oxygen free radical in the hepatocyte, and lipid peroxidation strengthens, and makes cell membrane and endomembrane system structural deterioration, causes hepatocyte injury; A large amount of liver endoenzymes (ALT, AST) are released into blood, can make to remove carrying out property of free radical enzyme (GSH-Px, SOD) exhaustion, further increase the weight of hepatic injury.At present, the research report that utilizes the Chinese medicine Antialcoholic liver-protecting is on a small quantity arranged, as application number 01131515.6, as application number 200810233671.7, application number 200710126799.9).
Summary of the invention
The objective of the invention is: herbal health care capsule of a kind of assisting in preventing and treating alcoholic liver injury and preparation method thereof is provided, extracts Chinese herbal medicine effective ingredients with the extractum form and produce capsule, help efficacy exertion, and preparation technology's simplification, raw material is easy to get.
Technical scheme of the present invention is: add vitamin C, vitamin E, vitamin B in the dry extract of Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae
1, B
6, filled capsules is made; Wherein, dry extract is made by following ingredients weight parts: 12～20 parts of Radix Puerariaes, 6～12 parts of Flos puerariae lobataes, 8～16 parts of Semen Hoveniae (Fructus Hoveniae)s, 4～8 parts of Herba Lysimachiae Clethroids, 2～6 parts of Fructus Crataegis, 1～3 part in Radix Glycyrrhizae.
The preparation method of health-caring capsule may further comprise the steps:
(1), crosses 20～30 mesh sieves with Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae pulverize separately;
(2) get coarse powder in following ratio: Radix Puerariae 10-20 part, Flos puerariae lobatae 6-12 part, Semen Hoveniae (Fructus Hoveniae) 8-16 part, Herba Lysimachiae Clethroids 4-8 part, Fructus Crataegi 2-6 part, Radix Glycyrrhizae 1-3 part, mixing gets mixed powder, mass concentration 50% ethanol that adds 8～12 times of amounts of mixed powder weight, twice of 70 ℃ of reflux, extract,, each 1～3 hour, filter, merge filtrate twice;
(3) filtrate that merges being evaporated to relative density under 50～60 ℃ is 1.35～1.40, gets extractum;
(4) with the extractum drying under reduced pressure, pulverize, cross 80～120 mesh sieves, get dry extract;
(5), add 16 parts of vitamin Cs, 8 parts of vitamin Es, vitamin B with dry extract 1000 parts of meters by weight
10.2 part, vitamin B
60.2 part, mixing is distributed into the capsule of content 0.3～0.5g.
The mechanism of action of each Chinese herbal medicine of the present invention:
Radix Puerariae is the dry root of the perennial defoliation liana of pulse family Pueraria lobota (Pueraria lobata (Willd) Ohwi.), belong to that Ministry of Public Health announces be food be again the article of medicine; Supplement to the Herbal is called its " alcoholic intoxication ", modern study shows, Radix Puerariae contains mineral, aminoacid, vitamin and the flavone of multiple needed by human, its main effective ingredient puerarin can significantly be removed reactive oxygen free radical, has multiple pharmacological effect such as treatment cardiovascular and cerebrovascular disease, prevention of arterial sclerosis, antioxidation, Antialcoholic liver-protecting.
Semen Hoveniae (Fructus Hoveniae) is the seed of the drying and ripening of Rhamnaceae plant Semen Hoveniae (Fructus Hoveniae) (Hovenia duleis Thunb.), the sweet acid of nature and flavor, flat, nontoxic, the effect that clearing away heat and promoting diuresis, alcoholic intoxication are arranged, cure mainly diseases such as drunk, dysphoria with smothery sensation, thirsty, vomiting, difficulty in urination and defecation, Semen Hoveniae (Fructus Hoveniae) contains multiple compositions such as saponin, alkaloid, flavonoid, have relieve the effect of alcohol, protect the liver, pharmacological action such as anti peroxidation of lipid, inhibition nervus centralis.
Flos puerariae lobatae is the flower of Herba Gelsemii Elegantis, the sweet cold of nature and flavor, and Compendium of Material Medica claims " alcoholic intoxication is good for the stomach and is amusing ", can be used for relieving the effect of alcohol and treats inappetence, vomiting etc.
Herba Lysimachiae Clethroids is the dry aerial parts of Saxifragaceae penthorum plant Herba Lysimachiae Clethroids (Penthorum chinense Pursh.).Beginning is stated from Ming Dynasty's herbal for Relief of Famines, and its property is flat, bitter in the mouth, little suffering, has heat clearing away, dampness removing, detoxifies, invigorates blood circulation, effects such as suppressing the hyperactive liver, spleen invigorating.
Fructus Crataegi is the dry mature fruit of rosaceous plant Fructus Pyri Pashiae or Fructus Crataegi (Crateagus pinnatifida L.), sour in the mouth, sweet, slightly warm in nature, effects such as removing food stagnancyization stagnates, convergence dysentery relieving, activating blood circulation to dissipate blood stasis are arranged, Fructus Crataegi contains multivitamin, malic acid, Crataegolic acid, Fructus Crataegi terpenoid, flavonoid etc., have significant blood vessel dilating and hypotensive effect, the function that strengthens cardiac muscle, arrhythmia, blood lipid regulation and cholesterol level is arranged.
Effective ingredient licoflavone in the Radix Glycyrrhizae, glycyrrhizin, glycyrrhizic acid etc. have the significant protection hepatocyte and improve the effect of liver function.
Vitamin can replenish people's a large amount of vitaminss that consume of back alcohol metabolism institute of drinking, and simultaneously, VC, VE have stronger antioxidation, and VB1, VB6 have the effect of neuroprotective cell, the damage that the minimizing alcohol metabolism causes body.
The present invention has the following advantages: be monarch drug with Radix Puerariae, the Semen Hoveniae (Fructus Hoveniae) that has than strong solution bartender liver function 1,, with Flos puerariae lobatae, Herba Lysimachiae Clethroids is ministerial drug, with Fructus Crataegi, Radix Glycyrrhizae is assistant, the pharmacological action that rationally utilizes these Chinese herbal medicine alcoholic intoxication, suppressing the hyperactive liver to protect the liver is prepared and is had the clear and definite significantly herbal health care capsule of the assisting in preventing and treating alcoholic liver injury of health-care effect; 2, extract the Chinese herbal medicine active ingredient with the extractum form, each composition Synergistic helps efficacy exertion; 3, raw material is easy to get, and technology is simple.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.Should be understood that these embodiment just in order to demonstrate the invention, but not limit the scope of the invention by any way.
Example 1: prepare capsule according to following steps:
(1), crosses 30 mesh sieves with Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae pulverize separately;
(2) get a powder in following ratio: 12 parts of Radix Puerariaes, 6 parts of Flos puerariae lobataes, 8 parts of Semen Hoveniae (Fructus Hoveniae)s, 4 parts of Herba Lysimachiae Clethroids, 2 parts of Fructus Crataegis, 1 part in Radix Glycyrrhizae, the coarse powder mixing gets mixed powder, mass concentration 50% ethanol that adds 8 times of amounts of mixed powder weight, twice of 70 ℃ of reflux, extract,, each 3 hours, filter, merge filtrate twice;
(3) filtrate that merges being evaporated to relative density under 50 ℃ is 1.40, gets extractum;
(4) with the extractum drying under reduced pressure, pulverize, cross 120 mesh sieves, get dry extract;
(5), add 16 parts of vitamin Cs, 8 parts of vitamin Es, vitamin B with dry extract 1000 parts of meters by weight
10.2 part, vitamin B
60.2 part, mixing is distributed into the capsule of content 0.3g.
Example 2: prepare capsule according to following steps:
(1), crosses 25 mesh sieves with Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae pulverize separately;
(2) get coarse powder in following ratio: 16 parts of Radix Puerariaes, 9 parts of Flos puerariae lobataes, 12 parts of Semen Hoveniae (Fructus Hoveniae)s, 6 parts of Herba Lysimachiae Clethroids, 4 parts of Fructus Crataegis, 2 parts in Radix Glycyrrhizae, the coarse powder mixing gets mixed powder, mass concentration 50% ethanol that adds 10 times of amounts of mixed powder weight, twice of 70 ℃ of reflux, extract,, each 2 hours, filter, merge filtrate twice;
(3) filtrate that merges being evaporated to relative density under 55 ℃ is 1.38, gets extractum;
(4) with the extractum drying under reduced pressure, pulverize, cross 100 mesh sieves, get dry extract;
(5), add 16 parts of vitamin Cs, 8 parts of vitamin Es, vitamin B with dry extract 1000 parts of meters by weight
10.2 part, vitamin B
60.2 part, mixing is distributed into the capsule of content 0.4g.
Example 3: prepare capsule according to following steps:
(1), crosses 30 mesh sieves with Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae pulverize separately;
(2) get coarse powder in following ratio: 20 parts of Radix Puerariaes, 12 parts of Flos puerariae lobataes, 16 parts of Semen Hoveniae (Fructus Hoveniae)s, 8 parts of Herba Lysimachiae Clethroids, 6 parts of Fructus Crataegis, 3 parts in Radix Glycyrrhizae, the coarse powder mixing gets mixed powder, mass concentration 50% ethanol that adds 12 times of amounts of mixed powder weight, twice of 70 ℃ of reflux, extract,, each 3 hours, filter, merge filtrate twice;
(3) filtrate that merges being evaporated to relative density under 60 ℃ is 1.40, gets extractum;
(4) with the extractum drying under reduced pressure, pulverize, cross 120 mesh sieves, get dry extract;
(5), add 16 parts of vitamin Cs, 8 parts of vitamin Es, vitamin B with dry extract 1000 parts of meters by weight
10.2 part, vitamin B
60.2 part, mixing is distributed into the capsule of content 0.5g.
1 toxicology test
1.1 test material
1.1.1 experimental animal
Secondary Kunming kind white mice and SD rat.
1.1.2 main medicine and reagent
The herbal health care capsule of assisting in preventing and treating alcoholic liver injury.
1.2 test method
1.2.1 health-caring capsule is handled
The strip off capsule shells is got content, and is water-soluble, filters.
1.2.2 acute toxicity test (LD
50Mensuration)
Get 60 of body weight 18～22g test white mice, be divided into 6 groups at random, 10 every group, male and female half and half; Adopt karber's method to carry out the acute toxicity test design, according to the trial test result, in conjunction with the criterion as a result of health food toxicology test, if 10000mg/kg is BW, 14953mg/kg BW, 22361mg/kg BW, 5 dosage groups of 33437mg/kg BW and 50000mg/kg BW (dosage is all in conversion crude drug amount, down together) and a blank group; Each is organized white mice and adopts gastric infusion, and capacity is 20mL/kg BW, and successive administration 7d observes white mice spirit, appetite, drinking-water, activity and poisoning situation every day, and the mortality of record white mice.
1.2.3Ames test
With mouse typhus salmonella bacteria histidine defect type mutant TA97, TA98, TA100 and TA102 are test organisms, adopt the plate method of mixing to carry out Salmonella reversion test, use through the inductive rat of Polychlorinated biphenyls (PCB mixture) to prepare liver homogenate as external activation system (S
9), test is divided into adds the S of metabolic activation system
9Do not add the two big group parallel tests of metabolic activation system; 7 test group are established in each big group test respectively, and wherein the A group is the solvent control group; B, C, D, E organize to being tried the thing group, and each is organized health-caring capsule dosage and is respectively 1000mg/ ware, 500mg/ ware, 250mg/ ware, 100mg/ ware; The F group is for returning the change matched group naturally; G organizes positive matched group; The test plate through 37 ℃ hatch 48h after, count every ware recovery mutation colony number.
1.2.4 mouse marrow cell micro nuclear test
Get 50 of body weight 24～26g test white mice, be divided into 5 groups at random, 10 every group, male and female half and half; The A group is the solvent control group; B, C, D organize to being tried the thing group, and each is organized health-caring capsule dosage and is respectively 2.5g/kg BW, 5.0g/kg BW, 10.0g/kg BW; E organizes positive matched group, irritates stomach by 40mg/kg BW dosage and gives cyclophosphamide; Being tried thing gives by 20mL/kg BW capacity filling stomach, give medicine for the second time behind the 24h of administration for the first time, put to death animal behind the 6h of administration for the second time, get the bone marrow of sternum smear, Giemsa dyeing, 1000 polychromatic erythrocyte numbers of every animal of observation under oily mirror, and the polychromatic erythrocyte number of micronucleus wherein appears in record.
1.2.5 mouse sperm deformity test
Get 50 of the male white mouses of body weight 25～30g, be divided into 5 groups at random, 10 every group, the A group is the solvent control group; B, C, D organize to being tried the thing group, and each is organized health-caring capsule dosage and is respectively 2.5g/kg BW, 5.0g/kg BW, 10.0g/kg BW; E organizes positive matched group, irritates stomach by 50mg/kg BW dosage and gives cyclophosphamide; Tried thing and cyclophosphamide and give by 20mL/kg BW capacity filling stomach, 5d continues to feed 30d afterwards continuously, puts to death animal then, gets the epididymal sperm smear, Yihong dyeing, 1000 complete sperms of observation under every animal high power lens, and record teratospermia number.
1.2.630 it feeding trial
Get 80 of the SD rats of body weight 80～90g, be divided into 4 groups at random, 20 every group, male and female half and half; The A group is matched group, irritates stomach by 10mL/kg BW capacity and gives normal saline; B, C, D organize to being tried the thing group, and each is organized health-caring capsule dosage and is respectively 5.0g/kg BW, 10.0g/kg BW, 20.0g/kg BW, irritates stomach respectively by 10mL/kg BW capacity and gives; Feed 30d continuously; Observe performance, behavior, poisoning symptom, death condition, body weight, the food utilization of animal every day; When finishing, experiment measures hemoglobin (Hb), red blood cell count(RBC) (RBC), numeration of leukocyte (WBC); Serum glutamic pyruvic transminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), serum urea nitrogen (BUN), T-CHOL (TCH), triglyceride (TG), creatinine (CRE), blood glucose (GLU), serum albumin (ALB), total protein (TP); Organ weights and coefficient also carry out check pathological section to organs such as liver,spleen,kidney, gastrointestinal, testis and ovaries.
1.2.7 statistical method
Adopt SPSS11.0 for windows statistical package, measurement data adopts one wayanvoa, and the numeration data adopts Norparamitric Tests.
1.3 result of the test
1.3.1 acute toxicity test
Observe through 7d, situation such as the spirit of Total Test white mice, appetite, drinking-water and matched group are more no abnormal, also do not have dead generation, the results are shown in Table 1; Determine white mice per os filling stomach health-caring capsule 50000mg/kg BW in view of the above, be equivalent to 117 times of human body recommended amounts 425mg/kg BW, intoxicating phenomenon still do not occur; Press the toxicological evaluation standard, can measure median lethal dose(LD 50) (LD
50), promptly can be considered LD
50＞50000mg/kg BW; The result shows that the Chinese herbal medicine hepatoprotective health-caring capsule of this project development belongs to actual non-toxic type material.
1.3.2Ames result of the test
Salmonella reversion test the results are shown in Table 2; Result of the test shows: returning naturally of (1) each bacterial strain negative control becomes the bacterium colony number average in normal allowed band; (2) health-caring capsule is to Salmonella typhimurium TA97, TA98, TA100, TA102 four strain test strains, when adding and do not add S9, each dosage group is returned and is become the bacterium colony number average above 2 times of negative control clump counts, does not also have dose-response relationship, and the Salmonella reversion test result is negative.
1.3.3 mouse marrow cell micro nuclear test
Mouse marrow cell micro nuclear test the results are shown in Table 3; The result shows, tried the micronuclear rates and the negative control group of each dosage group male and female sex of thing and more all do not had significant difference, being tried does not have significant difference between each dosage group of thing yet, tried each dosage group of thing and compared difference extremely significantly (P＜0.01) with positive controls, illustrated that to be tried thing bone marrow micronucleus test result negative.
Table 3 health-caring capsule mouse marrow cell micro nuclear test result
Annotate:
*Difference extremely significantly (P＜0.01) is compared in expression with negative control group
1.3.4 mouse sperm deformity test
The mouse sperm deformity result of the test sees Table 4; As shown in Table 4, tried the mouse sperm deformity rate of each dosage group of thing and organized the poor significant difference that all do not have with feminine gender, being tried does not have significant difference between each dosage group of thing yet, tried each dosage group of thing and compared difference extremely significantly (P＜0.01) with positive controls, can be judged that to be tried Chinese herb compound negative to the mouse sperm teratogenesis.
Table 4 health-caring capsule mouse sperm deformity test result of the test
Annotate:
*Difference extremely significantly (P＜0.01) is compared in expression with negative control group
1.3.530 it feeding trial
Various dose health-caring capsule feeding rat is after 30 days, and the rat hair is normal, no abnormal behavior performance, the no phenomena of mortality; Each dosage group body weight, food utilization and matched group more all do not have significant difference; To rat serum conventional index, blood biochemistry index inspection the results are shown in Table 5, table 6, the result shows: tried the rat serum conventional index of each dosage group of thing and blood biochemical investigation result and compare with matched group and all do not have significant difference.
After 30 days, the liver/body of each dosage group, spleen/body, kidney/body, testis/hierarchy number see Table 7 with health-caring capsule feeding rat, and the result shows and compares does not all have significant difference; Rat liver,spleen,kidney, gastrointestinal, testis, ovary are dissected, find that each each organ appearance color of dosage group male and female rat, size are normal, do not see obviously ooze out, pathological changes such as hypertrophy, edema, atrophy.The microscopic examination result shows that high dose group and normal control group compare, and the result all in the normal morphology scope, does not see that obvious toxic pathology changes.
Table 5 health-caring capsule is to the influence of rat serum conventional index
1.4 brief summary
1.4.1 acute toxicity test is to estimate medicine animal body is had the important indicator of free of toxic effects, result of the test shows that health-caring capsule is to the nontoxic level in the true border of kind white mice per os toxicity, secondary Kunming of two kinds of sexes.
1.4.2 three mutagenicity tests (Salmonella reversion test, mouse marrow cell micro nuclear test, mouse sperm deformity test) are mainly used in to estimate and are tried the genetoxic of thing and whether have potential carcinogenesis, result of the test shows that three results are all negative for this of health-caring capsule.
1.4.330 in it feeding trial, health-caring capsule does not all make significant difference to rat growthing development, every routine blood test index and blood parameters, substantive pathological change does not all take place in organ-tissues such as high dose group and rats in normal control group liver,spleen,kidney, gastrointestinal, testis and ovary, and result of the test shows the Chinese herbal medicine hepatoprotective health-caring capsule safety non-toxic of this project development.
2 effect evaluation tests
2.1 test material
2.1.1 tried health product
The herbal health care capsule of assisting in preventing and treating alcoholic liver injury.
2.1.2 experimental animal
The healthy male SD rat of cleaning level, body weight 160～180g.
2.1.3 main agents
Positive control medicine (FUFANG BIEJIA RUANGAN PIAN), commercially available 56 ° of Red Star strong, colourless liquor distilled from sorghum Chinese liquor, triacylglycerol (TG), glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), malonaldehyde (MDA), paddy Guang order peptide peroxidase (GSH-Px) are measured test kit, and other reagent are the analytical pure level.
2.1.4 key instrument
722 type grating spectrophotometers, TGL-16G type High speed refrigerated centrifuge, XS-212 type biologic photomicroscope.
2.2 test method
2.2.1 health-caring capsule is handled
The strip off capsule shells is got content, and is water-soluble, filters, and makes 50mg/mL solution.
2.2.2 animal grouping and processing
Will 100 SD rat adaptabilities be divided into 5 groups at random after raising 5d: 10 of (1) normal group, by every 15g raisings that feed intake every day, ad lib, drinking-water are given with other and organize isopyknic normal saline filling stomach every afternoons with conventional feed; (2) model group is 15, (conventional feed, cholesterol, Semen Maydis oil, pyrazoles were by 80: 1: 18.5: 0.5 mixed with the modeling feedstuff, again granulate behind the microwave heating) raise, ad lib, drinking-water, irritate stomach by 10mL/kg BW to Chinese liquor simultaneously, every morning and afternoon are respectively once, the Chinese liquor of giving mass concentration 20% the 1st～the 2nd week earlier is (with 56 ° of Red Star strong, colourless liquor distilled from sorghum Chinese liquor and distilled water preparation, down together), the the 3rd～the 4th week was given the Chinese liquor of mass concentration 40%, rose in the 5th week directly with 56 ° of Red Star strong, colourless liquor distilled from sorghum Chinese liquor until off-test; (3) positive controls is 15, on the basis of model group, irritates stomach at 12 o'clock at noon every day isopyknic FUFANG BIEJIA RUANGAN PIAN [1g/ (kgd)] aqueous solution again; (4) the large and small dosage group of health-caring capsule is each 15, and on the basis of model group, irritate the solution that stomach gives the preparation of Chinese herbal medicine hepatoprotective health-caring capsule by 20mL/kg BW, 10mL/kg BW respectively at 12 o'clock at noon every day again; Each is organized rat and plays the 1st day, the 4th weekend, the 8th weekend, the 12nd weekend respectively at modeling and weigh, and extracts femoral vein blood and put to death behind fasting at the 12nd weekend 16h, leaves and takes the liver of respectively organizing rat respectively and is used for detecting.
2.2.3 observation index and method
(1) pathological change and lipidosis situation are observed in liver organization HE dyeing, and reference literature is judged steatosis degree integration; (2) detection of ALT and AST: get separation of whole blood serum, respectively with ALT and AST activity in reitman-frankel method and the colorimetry detection serum; (3) GSH-Px activity and TG, MDA Determination on content in the hepatic tissue: get an amount of liver organization, make 10% liver homogenate, get supernatant after centrifugal, press the biochemical reagents box and measure GSH-Px activity and TG, MDA content with after the cold saline flushing.
2.2.4 statistical method
Measurement data represents with x ± s that all numerical variables all adopt SPSS11.0 to carry out one factor analysis of variance (One-way ANOVA), relatively adopts the t check between group, determines that with P＜0.05 difference has statistical significance.
2.3 result of the test
2.3.1 animal ordinary circumstance
After 4 weeks of on-test, normal rats is smooth by hair, and is active, weight increase; It is loose fluffy and disorderly by hair that all the other respectively organize rat, and tarnish is movable few, lethargy, and urine colour cast Huang, the part rat defecates rare soft, and weight increase is slow than normal group, but compares with model group, and part is tried thing group rat symptom and is taken a turn for the better; Normal rats is all survived, and all the other each group has 12 rats deaths, wherein 3 because of liquid to pour into trachea dead immediately; Die from pulmonary venous pleonaemia and pulmonary infection for 5, due to aspiration pneumonitis; 2 because of the flatulence perforation; 2 unknown causes; Each body weight change and liver index of organizing rat sees Table 8.
Table 8 is respectively organized relatively (x ± s) of rat liver index and body weight change
Annotate: compare with normal group, △ represents P＜0.01; Compare with model group,
*Expression P＜0.05,
*Expression P＜0.01.
2.3.2 hepatic tissue pathology changes
Normal rat liver tunicle is smooth, the quality softness, and the lobules of liver structural integrity is not seen steatosis and inflammatory cell infiltration; The enlargement of model group rat liver outward appearance, the lobules of liver structural deterioration, visible point-like or focal necrosis, visible significant quantities of fat degeneration in the hepatocyte, hepatic tissue has the inflammatory cell infiltration of more amount; Positive controls and tried thing and respectively organize the rat fat degeneration and compare obviously with model group with the inflammation degree and alleviate, the lobules of liver structure is normal substantially, and the steatosis integration is starkly lower than model group, sees Table 9.
2.3.3 respectively organize the comparison of liver tissues of rats TG content
Compare with normal group, the model group rat liver TG content (P＜0.01=that significantly raises, heavy dose of Chinese herbal medicine hepatoprotective health-caring capsule can significantly suppress rat liver TG content rising (P＜0.01=, obvious inhibition is also arranged during smaller dose, and (P＜0.05=sees Table 9; Illustrate that Chinese herbal medicine hepatoprotective health-caring capsule can protect hepatocyte, promote the metabolism of liver, alcoholic liver injury is had protective effect fat.
Table 9 is respectively organized relatively (x ± s) of liver tissues of rats TG content and steatosis integration
Annotate: compare with normal group, △ represents P＜0.01; Compare with model group,
*Expression P＜0.05,
*Expression P＜0.01; Compare ▲ expression P＜0.05 with positive controls.
2.3.4 respectively organize the comparison of rat blood serum ALT, AST activity level
Model group rat blood serum ALT, AST activity are significantly higher than normal group, and ALT, the AST activity of respectively organizing rat blood serum after employing medicine and the intervention of Chinese herbal medicine hepatoprotective health-caring capsule have remarkable reduction than model group, see Table 10; The large and small dosage group difference of Chinese herbal medicine hepatoprotective health-caring capsule is remarkable (P＞0.05) not.
Table 10 is respectively organized relatively (x ± s) of rat blood serum ALT, AST activity level
Annotate: compare with normal group, △ represents P＜0.01; Compare with model group,
*Expression P＜0.05,
*Expression P＜0.01; Compare ▲ expression P＜0.05 with positive controls.
2.3.5 respectively organize the comparison of liver tissues of rats peroxidating index
With normal group relatively, model group liver tissues of rats MDA content significantly raises, the GSP-Px activity level (P＜0.01=that significantly descends; And compare with model group, positive controls, the large and small dosage group of Chinese herbal medicine hepatoprotective health-caring capsule liver tissues of rats MDA content all obviously reduce, the GSP-Px activity level obviously raises, and the heavy dose of group of Chinese herbal medicine hepatoprotective health-caring capsule liver tissues of rats GSP-Px activity level can see Table 11 near normal level; The large and small dosage group difference of Chinese herbal medicine hepatoprotective health-caring capsule is remarkable (P＞0.05) not, illustrates that Chinese herbal medicine hepatoprotective health-caring capsule can be by removing free radical, lipoid peroxidization resistant the liver protecting.
Table 11 is respectively organized relatively (x ± s) of rat blood serum MDA, GSP-Px activity level
Annotate: compare with normal group, △ represents P＜0.01; Compare with model group,
*Expression P＜0.05,
*Expression P＜0.01.
2.4 brief summary
Alcoholic liver injury is clinical common hepatic injury.Long-term heavy drinking can produce a large amount of reactive oxygen free radical in the hepatocyte, and lipid peroxidation strengthens, and makes cell membrane and endomembrane system structural deterioration, causes hepatocyte injury; A large amount of liver endoenzymes (ALT, AST) are released into blood, can make to remove carrying out property of free radical enzyme (GSH-Px, SOD) exhaustion, further increase the weight of hepatic injury.
Test shows that ALT, AST are active in the alcoholic liver injury rat blood serum significantly raises, and its liver index, hepatic tissue TG content, MDA content, hepatic tissue steatosis degree all are significantly higher than normal rats, and the GSH-Px enzymatic activity significantly reduces; After adopting Chinese herbal medicine hepatoprotective health-caring capsule to intervene, ALT and AST activity, liver index, hepatic tissue TG content significantly reduce than model group in the rat blood serum, the hepatic tissue pathology degeneration is clearly better, and illustrates that Chinese herbal medicine hepatoprotective health-caring capsule has the protection hepatocyte, alleviates the effect of hepatocyte fat accumulation; The result also shows, Chinese herbal medicine hepatoprotective health-caring capsule can significantly reduce the accumulation of the lipid peroxidation end-product MDA that metabolism causes in liver by ethanol, make GSH-Px enzymatic activity rebound significantly, illustrate that Chinese herbal medicine hepatoprotective health-caring capsule can remove free radical effectively, suppress lipid peroxidation, thereby improve the hepatic tissue oxidation resistance.
Comprehensive above the analysis; the pharmacological action that the present invention has rationally utilized Chinese herbal medicine alcoholic intoxication, suppressing the hyperactive liver such as Radix Puerariae, Semen Hoveniae (Fructus Hoveniae), Flos puerariae lobatae, Herba Lysimachiae Clethroids to protect the liver; the alcoholic liver injury rat had significant protective effect; its protection mechanism is that effective ingredient has and improve to remove free radical enzyme (GSH-Px, SOD) active function in the Chinese herbal medicine, can suppress the hepatic injury due to the lipid peroxidation of free radical mediated.
Claims (2)
1, the herbal health care capsule of assisting in preventing and treating alcoholic liver injury is characterized in that: add vitamin C, vitamin E, vitamin B in the dry extract of Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae
1, B
6, filled capsules is made; Wherein, dry extract is made by following ingredients weight parts: 12～20 parts of Radix Puerariaes, 6～12 parts of Flos puerariae lobataes, 8～16 parts of Semen Hoveniae (Fructus Hoveniae)s, 4～8 parts of Herba Lysimachiae Clethroids, 2～6 parts of Fructus Crataegis, 1～3 part in Radix Glycyrrhizae.
2, the capsular preparation method of herbal health care of the described assisting in preventing and treating alcoholic liver injury of a kind of claim 1 is characterized in that it may further comprise the steps:
(1), crosses 20～30 mesh sieves with Radix Puerariae, Flos puerariae lobatae, Semen Hoveniae (Fructus Hoveniae), Herba Lysimachiae Clethroids, Fructus Crataegi, Radix Glycyrrhizae pulverize separately;
(2) get coarse powder in following ratio: Radix Puerariae 10-20 part, Flos puerariae lobatae 6-12 part, Semen Hoveniae (Fructus Hoveniae) 8-16 part, Herba Lysimachiae Clethroids 4-8 part, Fructus Crataegi 2-6 part, Radix Glycyrrhizae 1-3 part, the coarse powder mixing gets mixed powder, mass concentration 50% ethanol that adds 8～12 times of amounts of mixed powder weight, twice of 70 ℃ of reflux, extract,, each 1～3 hour, filter, merge filtrate twice;
(3) filtrate that merges being evaporated to relative density under 50～60 ℃ is 1.35～1.40, gets extractum;
(4) with the extractum drying under reduced pressure, pulverize, cross 80～120 mesh sieves, get dry extract;
(5), add 16 parts of vitamin Cs, 8 parts of vitamin Es, vitamin B with dry extract 1000 parts of meters by weight
10.2 part, vitamin B
60.2 part, mixing is distributed into the capsule of content 0.3～0.5g.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100330566A CN101579421A (en) | 2009-06-11 | 2009-06-11 | Chinese herbal medicinal health capsule for assisting in preventing and treating alcoholic liver injury, and preparation method thereof |
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| CNA2009100330566A CN101579421A (en) | 2009-06-11 | 2009-06-11 | Chinese herbal medicinal health capsule for assisting in preventing and treating alcoholic liver injury, and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969588A (en) * | 2017-10-30 | 2018-05-01 | 陈茂锐 | A kind of sugar-free sweet Antialcoholic liver-protecting coralhead plant stem with leaf plant beverage and preparation method thereof |
| CN108634315A (en) * | 2018-05-22 | 2018-10-12 | 湖北康科德生物科技有限公司 | One kind is relieved the effect of alcohol soft capsule and preparation method thereof |
| CN110179854A (en) * | 2019-07-08 | 2019-08-30 | 重庆霏洋环保科技股份有限公司 | A kind of formula reducing alcohol side effect preparation |
-
2009
- 2009-06-11 CN CNA2009100330566A patent/CN101579421A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107969588A (en) * | 2017-10-30 | 2018-05-01 | 陈茂锐 | A kind of sugar-free sweet Antialcoholic liver-protecting coralhead plant stem with leaf plant beverage and preparation method thereof |
| CN108634315A (en) * | 2018-05-22 | 2018-10-12 | 湖北康科德生物科技有限公司 | One kind is relieved the effect of alcohol soft capsule and preparation method thereof |
| CN110179854A (en) * | 2019-07-08 | 2019-08-30 | 重庆霏洋环保科技股份有限公司 | A kind of formula reducing alcohol side effect preparation |
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Open date: 20091118 |