CN105628552A - Fermentation detection method and device - Google Patents

Fermentation detection method and device Download PDF

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Publication number
CN105628552A
CN105628552A CN201510915262.5A CN201510915262A CN105628552A CN 105628552 A CN105628552 A CN 105628552A CN 201510915262 A CN201510915262 A CN 201510915262A CN 105628552 A CN105628552 A CN 105628552A
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China
Prior art keywords
fermentation
fermented product
buoyancy
sensor
lift heavy
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CN201510915262.5A
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CN105628552B (en
Inventor
付沈斌
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Guangzhou Guorui Scientific Instrument Co Ltd
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Guangzhou Guorui Scientific Instrument Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N9/00Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity
    • G01N9/08Investigating density or specific gravity of materials; Analysing materials by determining density or specific gravity by measuring buoyant force of solid materials by weighing both in air and in a liquid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/36Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Abstract

The invention provides a fermentation detection method. The fermentation detection method comprises a fermenter accommodating a fermentation object, and a rubber plug used for sealing is mounted in the opening of the fermenter; a mounting box is arranged on the opening of the fermenter; a circuit board and a sensor are mounted in the mounting box, a microcontroller is mounted on the circuit board, the sensor is connected with a rigid wire, the rigid wire penetrates the rubber plug and is connected with a hanging heavy by a flexible wire, and the hanging heavy is immersed in the fermentation object. A test method of a detector comprises the following steps: a hanging heavy buoyancy data acquisition step; a data processing step; a result display step. The hanging heavy is immersed in the fermenter, and changes of the buoyancy of the hanging heavy during fermentation of the fermentation object are acquired by the sensor and converted into changes of voltage to detect the fermentation degree of the fermentation object, so that automatic detection is realized, both manpower and material resources are saved, and the detection efficiency is greatly improved.

Description

A kind of fermentation detection method and detecting device
Technical field
The invention belongs to technical field of mechanical equipment, be specifically related to a kind of fermentation detection method and detecting device.
Background technology
Fermentation, refers to that the electronics that oxidation operation is discharged by microbial cell directly gives certain intermediate product of the non-complete oxidation of substrate itself, releases energy simultaneously and produce various different metabolite. Under fermentation conditions, Organic substance is partly oxidized, therefore, only discharges a fraction of energy. The oxidation of sweat is linked togather with organic reduction Rhizoma Nelumbinis. The Organic substance being reduced comes from the catabolism of Preliminary fermentation, namely need not provide electron acceptor in the external world. The modern definition to fermentation is: by the grown cultures of microorganism (or animal and plant cells) and chemical change, produce and accumulate the course of reaction of special metabolite in a large number.
Can there is certain change in change attenuation degree over time during the fermentation, needs sporadically to test attenuation degree to better control over sweat. The method of existing detection attenuation degree is all detect attenuation degree by the artificial fermentation liquid that gathers of timing, this method of testing labor intensive material resources, detection efficiency is low, is sporadically acquired fermented product fermented product causing certain pollution simultaneously, affects fermentation results.
Summary of the invention
The object of the invention thing overcomes the deficiencies in the prior art, it is provided that can automatically detect attenuation degree, and detection efficiency is high, free of contamination fermentation detection method and detecting device.
The technical scheme is that a kind of fermentation detection method, including the fermenter equipped with fermented product, the bung of described fermenter, less than the diameter of ladle body, is provided with the rubber stopper for sealing in described bung; Described bung is provided with mounting box, being provided with circuit board and sensor in described mounting box, described circuit board is provided with microcontroller, described sensor is connected to rigid line, described rigid line is connected to lift heavy thing through described rubber stopper by flexible cord, and described lift heavy thing is immersed in described fermented product; Wherein, the method for testing of this detector comprises the steps:
Lift heavy thing buoyancy data collection steps: described lift heavy thing is immersed in fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change along with fermented product concentration change in the buoyancy of described lift heavy thing, and described sensor is for gathering the size of described lift heavy thing buoyancy;
The switch process of buoyancy data: the size of described buoyancy changes into the size of voltage by sensor, then voltage data is transferred to again the microcontroller on described circuit board by described sensor;
Voltage data processes step: described microcontroller internal memory contains alcohol concentration calibration equation, and described microcontroller is by carrying out comparing calculation thus drawing the concentration of described ethanol by the voltage data of reception and calibration equation;
Fermentation results step display: the result of calculated described alcohol concentration is transferred on display screen and displays.
Preferably, described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition and statistics.
Preferably, described display screen is arranged on the upper surface of described mounting box.
Preferably, the side of described display screen is provided with air-lock cup, is provided with in air-lock cup in described air-lock cup.
Preferably, in described air-lock, cup has breather line with shape in described fermenter.
A kind of fermentation detecting device, including the fermenter equipped with fermented product, the bung of described fermenter, less than the diameter of ladle body, is provided with the rubber stopper for sealing in described bung; Described bung is provided with mounting box, being provided with circuit board and sensor in described mounting box, described circuit board is provided with microcontroller, described sensor is connected to rigid line, described rigid line is connected to lift heavy thing through described rubber stopper by flexible cord, and described lift heavy thing is immersed in described fermented product; Wherein, this detector also includes such as lower module:
Lift heavy thing buoyancy data acquisition module: described lift heavy thing is immersed in fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change, the size of lift heavy thing buoyancy described in described sensor acquisition along with fermented product concentration change in the buoyancy of described lift heavy thing;
The switch process of buoyancy data: the size of described buoyancy changes into the size of voltage by sensor, then voltage data is transferred to again the microcontroller on described circuit board by described sensor;
Voltage data processing module: described microcontroller internal memory contains alcohol concentration calibration equation, described microcontroller is by carrying out comparing calculation thus drawing the concentration of described ethanol by the voltage data of reception and calibration equation;
Fermentation results display module: the result of calculated described alcohol concentration is transferred on display screen and displays.
Preferably, described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition and statistics.
Preferably, described display screen is arranged on the upper surface of described mounting box.
Preferably, the side of described display screen is provided with air-lock cup, is provided with in air-lock cup in described air-lock cup.
Preferably, in described air-lock, cup has breather line with shape in described fermenter.
Beneficial effects of the present invention: present invention submergence in fermenter has lift heavy thing, by the change of buoyancy in described fermented product attenuation degree of lift heavy thing described in sensor acquisition, and the change of buoyancy is converted into voltage change thus detecting the attenuation degree of fermented product, described in whole test process, fermenter is all undertaken hermetically sealed by rubber stopper, thus without there being any pollution, the mode of this automatic detection saves manpower and materials simultaneously, substantially increases detection efficiency.
Accompanying drawing explanation
The overall structure schematic diagram of a kind of detecting device that ferments of Fig. 1 present invention.
The flow chart of steps of a kind of detection method of fermenting of Fig. 2 present invention.
The theory structure block diagram of a kind of detecting device that ferments of Fig. 3 present invention.
The label that in figure, each component names is corresponding: fermenter-1, rubber stopper-2, mounting box-3, circuit board-4, sensor-5, microcontroller-6, rigid line-7, flexible cord-8, lift heavy thing-9, display screen-10, air-lock cup-11, cup-12 in air-lock, breather line-13.
Detailed description of the invention
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described further
As depicted in figs. 1 and 2, a kind of fermentation detection method, including the fermenter 1 equipped with fermented product, the bung of described fermenter 1, less than the diameter of ladle body, is provided with the rubber stopper 2 for sealing in described bung; Described bung is provided with mounting box 3, circuit board 4 and sensor 5 are installed in described mounting box 3, described circuit board 4 is provided with microcontroller 6, described sensor 5 is connected to rigid line 7, described rigid line 7 is connected to lift heavy thing 9 through described rubber stopper 2 by flexible cord 8, and described lift heavy thing 9 is immersed in described fermented product; Wherein, the method for testing of this detector comprises the steps:
Lift heavy thing buoyancy data collection steps: described lift heavy thing is immersed in described fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change along with fermented product concentration change in the buoyancy of described lift heavy thing, and described sensor is for gathering the size of described lift heavy thing buoyancy;
The switch process of buoyancy data: the size of described buoyancy changes into the size of voltage by sensor place, then voltage data is transferred to again the microcontroller on described circuit board by described sensor;
Voltage data processes step: described microcontroller internal memory contains alcohol concentration calibration equation, and described microcontroller is by carrying out comparing calculation thus drawing the concentration of described ethanol by the voltage data of reception and alcohol concentration calibration equation;
Fermentation results step display: the result of calculated described alcohol concentration is transferred on display screen 10 and displays.
Described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition and statistics, detect mainly by artificial the carrying out periodically extracting fermented product, result according to detection sets up the function model of an attenuation degree and time, thus obtaining alcohol concentration calibration equation, the calibration equation of different fermented products is different from.
Described display screen 10 is arranged on the upper surface of described mounting box 3. The side of described display screen 10 is provided with air-lock cup 11, is provided with in air-lock cup 12 in described air-lock 11 glasss, and in described air-lock, cup 12 has breather line 13 with shape in described fermenter. Having air-flow to pass through in described mounting box 3 and air-lock cup 11, described breather line 13 makes the air pressure in described fermenter 1 consistent with the air pressure in described air-lock cup 11, but does not have environmental contaminants and entered in described fermenter by described breather line 13.
As shown in figures 1 and 3, a kind of fermentation detecting device, including the fermenter 1 equipped with fermented product, the bung of described fermenter 1, less than the diameter of ladle body, is provided with the rubber stopper 2 for sealing in described bung; Described bung is provided with mounting box 3, circuit board 4 and sensor 5 are installed in described mounting box 3, described circuit board 4 is provided with microcontroller 6, described sensor 5 is connected to rigid line 7, described rigid line 7 is connected to lift heavy thing 9 through described rubber stopper 2 by flexible cord 8, and described lift heavy thing 9 is immersed in described fermented product; Wherein, this detector also includes such as lower module:
Lift heavy thing buoyancy data acquisition module: described lift heavy thing is immersed in described fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change along with fermented product concentration change in the buoyancy of described lift heavy thing, and described sensor is for gathering the size of described lift heavy thing buoyancy;
The modular converter of buoyancy data: the size of described buoyancy changes into the size of voltage by sensor place, then voltage data is transferred to again the microcontroller on described circuit board by described sensor;
Voltage data processing module: described microcontroller internal memory contains alcohol concentration calibration equation, described microcontroller is by carrying out comparing calculation thus drawing the concentration of described ethanol by the voltage data of reception and calibration equation;
Fermentation results display module: the result calculating obtained described alcohol concentration is transferred on display screen 10 and displays.
Described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition and statistics, detect mainly by artificial the carrying out periodically extracting fermented product, result according to detection sets up the function model of an attenuation degree and time, thus obtaining alcohol concentration calibration equation, the calibration equation of different fermented products is different from.
Described display screen 10 is arranged on the upper surface of described mounting box 3, and the side of described display screen 10 is provided with air-lock cup 11, is provided with in air-lock cup 12 in described air-lock cup 11, and in described air-lock, cup 12 has breather line 13 with shape in described fermenter. Having air-flow to pass through in described mounting box 3 and air-lock cup 11, described breather line 13 makes the air pressure in described fermenter 1 consistent with the air pressure in described air-lock cup 11, but does not have environmental contaminants and entered in described fermenter by described breather line 13.
When using the present invention, fermented product being placed in fermentation cask fermentation, lift heavy thing is immersed in fermented product, then rubber stopper is stoppered, mounting box is arranged on described fermenter, and lift heavy thing is connected with the sensor in described mounting box, mounting box is installed air-lock cup. After fermented product starts fermentation, described sensor can sense the size of lift heavy thing buoyancy, and the size that buoyancy data are converted into voltage is sent on circuit board, being compared by described voltage swing and circuit board internal calibration formula and draw the result of attenuation degree, described result is intuitively shown by display screen.
The announcement of book and instruction according to the above description, above-mentioned embodiment can also be modified and revise by those skilled in the art in the invention. Therefore, the invention is not limited in detailed description of the invention disclosed and described above, some modifications and changes of invention be should also be as falling in the scope of the claims of the present invention. Although additionally, employ some specific terms in this specification, but these terms are intended merely to convenient explanation, and the present invention does not constitute any restriction.

Claims (10)

1. a fermentation detection method, including the fermenter equipped with fermented product, it is characterised in that be provided with the rubber stopper for sealing in described bung; Described bung is provided with mounting box, being provided with circuit board and sensor in described mounting box, described circuit board is provided with microcontroller, described sensor is connected to rigid line, described rigid line is connected to lift heavy thing through described rubber stopper by flexible cord, and described lift heavy thing is immersed in described fermented product; Wherein, the method for testing of this detector comprises the steps:
Lift heavy thing buoyancy data collection steps: described lift heavy thing is immersed in fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change, the size of lift heavy thing buoyancy described in described sensor acquisition along with fermented product concentration change in the buoyancy of described lift heavy thing;
The switch process of buoyancy data: the size of the buoyancy collected changes into the size of voltage by described sensor, then the voltage data after conversion is transferred to the microcontroller on described circuit board again by sensor;
Voltage data processes step: described microcontroller internal memory contains alcohol concentration calibration equation, and described microcontroller is by carrying out comparing calculation thus drawing the concentration of described ethanol by the voltage data of reception and alcohol concentration calibration equation;
Fermentation results step display: the result of calculated described alcohol concentration is transferred on display screen and displays.
2. a kind of fermentation detecting method as claimed in claim 1, it is characterised in that described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition, statistics and calculating.
3. a kind of fermentation detecting method as claimed in claim 1, it is characterised in that described display screen is arranged on the upper surface of described mounting box.
4. fermentation detecting method as claimed in claim 1 a kind of, it is characterised in that the side of described display screen is provided with air-lock cup, is provided with in air-lock in described air-lock cup cup.
5. a kind of fermentation detecting method as claimed in claim 1, it is characterised in that in described air-lock, cup has breather line with shape in described fermenter.
6. a fermentation detecting device, including the fermenter equipped with fermented product, it is characterised in that be provided with the rubber stopper for sealing in described bung; Described bung is provided with mounting box, being provided with circuit board and sensor in described mounting box, described circuit board is provided with microcontroller, described sensor is connected to rigid line, described rigid line is connected to lift heavy thing through described rubber stopper by flexible cord, and described lift heavy thing is immersed in fermented product; Wherein, this detector also includes such as lower module:
Lift heavy thing buoyancy data acquisition module: described lift heavy thing is immersed in described fermented product, change along with fermented product attenuation degree, the density of fermented product can change, therefore can there is corresponding change, the size of lift heavy thing buoyancy described in described sensor acquisition along with fermented product concentration change in the buoyancy of described lift heavy thing;
The modular converter of buoyancy data: the size of described buoyancy changes into the size of voltage by sensor, then the voltage data after conversion is transferred to the microcontroller on described circuit board again by described sensor;
Voltage data processing module: described microcontroller internal memory contains alcohol concentration calibration equation, described microcontroller is by carrying out comparing calculation by the alcohol concentration calibration equation of the voltage data of reception Yu storage thus drawing the concentration of described ethanol;
Fermentation results display module: the result of calculated alcohol concentration is transferred on display screen and displays.
7. a kind of fermentation detecting device as claimed in claim 6, it is characterised in that described alcohol concentration calibration equation is by obtaining after carrying out substantial amounts of data acquisition, statistics and calculating.
8. a kind of fermentation detecting device as claimed in claim 6, it is characterised in that described display screen is arranged on the upper surface of described mounting box.
9. fermentation detecting device as claimed in claim 6 a kind of, it is characterised in that the side of described display screen is provided with air-lock cup, is provided with in air-lock in described air-lock cup cup.
10. a kind of fermentation detecting device as claimed in claim 6, it is characterised in that in described air-lock, cup has breather line with shape in described fermenter.
CN201510915262.5A 2015-12-10 2015-12-10 fermentation detection method and detection device Active CN105628552B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108841480A (en) * 2018-06-29 2018-11-20 贵州省仁怀市水码头酿酒作坊 Installation for fermenting

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0517545U (en) * 1991-08-19 1993-03-05 関東科学サービス株式会社 Sample lifting tool for solid specific gravity measuring device
CN2681123Y (en) * 2004-03-16 2005-02-23 王鸿昌 Buoyancy type on-line digital alcohol content detecting device
CN2926236Y (en) * 2006-02-08 2007-07-25 河北科技大学 Filling supplement feeder of fermenter
CN201097192Y (en) * 2007-09-03 2008-08-06 吴行友 Distilled spirit online detection dosage control device
CN102796663A (en) * 2012-07-19 2012-11-28 南京荣欣化工有限公司 Ventilating fermentation tank
CN202661349U (en) * 2012-03-14 2013-01-09 雅化集团内蒙古柯达化工有限公司 Automatic monitoring system for solution concentration variation
CN102901795A (en) * 2012-10-16 2013-01-30 熊鹏 Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation
CN103134738A (en) * 2011-11-22 2013-06-05 李红 On-line alcohol degree testing method in liquor processing process

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0517545U (en) * 1991-08-19 1993-03-05 関東科学サービス株式会社 Sample lifting tool for solid specific gravity measuring device
CN2681123Y (en) * 2004-03-16 2005-02-23 王鸿昌 Buoyancy type on-line digital alcohol content detecting device
CN2926236Y (en) * 2006-02-08 2007-07-25 河北科技大学 Filling supplement feeder of fermenter
CN201097192Y (en) * 2007-09-03 2008-08-06 吴行友 Distilled spirit online detection dosage control device
CN103134738A (en) * 2011-11-22 2013-06-05 李红 On-line alcohol degree testing method in liquor processing process
CN202661349U (en) * 2012-03-14 2013-01-09 雅化集团内蒙古柯达化工有限公司 Automatic monitoring system for solution concentration variation
CN102796663A (en) * 2012-07-19 2012-11-28 南京荣欣化工有限公司 Ventilating fermentation tank
CN102901795A (en) * 2012-10-16 2013-01-30 熊鹏 Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108841480A (en) * 2018-06-29 2018-11-20 贵州省仁怀市水码头酿酒作坊 Installation for fermenting
CN108841480B (en) * 2018-06-29 2022-02-01 贵州省仁怀市水码头酿酒作坊 Fermentation device

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