CN102901795A - Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation - Google Patents
Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation Download PDFInfo
- Publication number
- CN102901795A CN102901795A CN2012103913991A CN201210391399A CN102901795A CN 102901795 A CN102901795 A CN 102901795A CN 2012103913991 A CN2012103913991 A CN 2012103913991A CN 201210391399 A CN201210391399 A CN 201210391399A CN 102901795 A CN102901795 A CN 102901795A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- real time
- concentration
- alcohol
- alcohol concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a simple method for determining alcohol concentration of a fermentation broth in real time during alcoholic fermentation, the theory that 1 mol CO2 is produced when 1 mol alcohol is produced during the alcoholic fermentation is utilized, and the quantity of production of CO2 is determined in real time during fermentation to determine the alcohol concentration of the fermentation broth. The determining method disclosed by the invention has the advantages of simplicity, rapidity for operation and accuracy in determination, and the alcohol concentration of the fermentation broth is reflected in real time.
Description
Technical field
The present invention relates to assay method, the method for fermentation liquor alcohol concentration in a kind of easy the real time measure alcoholic fermentation process of concrete steps.
Technical background
Ethanol claims again alcohol, as large chemical products be widely used in petrochemical complex, extract separate, the industry such as medicine, in recent years along with the petering out of fossil energy, ethanol as new forms of energy by year by year attention.
At present the mode of production of alcohol has two kinds of solid fermentation and liquid deep layer fermentings, and the former is mainly used in drinks and brewages, and the latter is the mode of production of main chemical industry, medical alcohol.Be that glucose is monose by degradable starch or cellulose in the alcoholic fermentation process, under oxygen-free environment, utilize alcoholic fermentation bacterial strain (the being mainly saccharomyces cerevisiae) anaerobic fermentation after the improvement to produce alcohol.Its chemical equation is:
Detection normal operation in the alcoholic fermentation process be high performance liquid chromatography, vapor-phase chromatography, by detecting in advance the alcohol standard specimen of concentration known, carry out concentration by peak area and calculate according to retention time is qualitative.This method is quantitatively accurate, simple to operate, but its checkout equipment is expensive, testing cost is high, detects simultaneously ceaselessly sampling and causes easily the fermentation tank microbiological contamination.In addition for middle-size and small-size laboratory and factory, the method that adopts chromatogram to detect is measured and must be introduced corresponding equipment, but the price of its apparatus expensive has caused the problem of detection difficult so that Some Enterprises, laboratory are hung back.
Summary of the invention
The object of the invention is to: the method for fermentation liquor alcohol concentration in a kind of easy the real time measure alcoholic fermentation process is provided, and method is simple and easy, and is swift to operate, measures accurately, reflects in real time alcohol concentration in the fermentation tank.
Technical solution of the present invention is: utilize that every generation 1mol alcohol produces 1mol CO simultaneously in the alcoholic fermentation process
2Principle, by CO in the real time measure sweat
2Generation measure alcohol concentration in the fermentation liquor; Concrete assay method may further comprise the steps:
1) according to the throwing amount in C source in the sweat to the theoretical CO that can produce in the sweat
2Calculate;
2) theory according to step 1) calculating gained produces CO
2The excessive alkali lye of molar weight configuration guarantee fully and the carbon dioxide generation neutralization reaction that produces, its OH
-Concentration is concentration known C
1, volume is V
1
3) in the alcoholic fermentation process gas exhaust duct is inserted the CO that also stirs the assurance generation in the alkali lye
2Can be in time and the abundant mixing of alkali lye and neutralization reaction occurs;
Volume aspirated V when 4) detecting in real time
2With the alkali lye of carbon dioxide generation neutralization reaction, and use known H
+Concentration C
3Acidometric titration to pH=7, read acid and use volume V
3
5) alcohol concentration C in the sweat
0Calculating be to calculate gained by following formula:
, wherein V is that fermentation cylinder for fermentation liquid is long-pending.
Wherein, the mass concentration g/L of alcohol is the volumetric molar concentration C of step 5) gained in the fermentation liquor
0Multiply by 46.
Wherein, the C source described in the step 1) refers to one or more potpourri of wheat, cassava, cornstarch class material or agricultural crop straw, careless class, forest biomass fiber raw material.
Wherein, the alkali step 2) refers to one or more potpourris in potassium hydroxide, NaOH, ammoniacal liquor, the calcium hydroxide.
Wherein, the acid described in the step 4) refers to one or more potpourris in hydrochloric acid, sulfuric acid, sulphurous acid, nitric acid, phosphoric acid, the carbonic acid.
Wherein, the CO that fermentation is produced
2Accounting is to be used for fermentation alcohol fully by calculating the C source, and conversion ratio is 100% CO that produces
2Molar weight realize.
Wherein, related concentration is volumetric molar concentration among the step 1-5.
Wherein, draw in the step 4 be used for the volume of neutralization reaction should be as far as possible little, can not cause large impact to result of calculation with the variation that guarantees volume, it is unchanged to be defaulted as the alkali lye volume in the computation process.
Characteristics of the present invention are: an easy method that detects in real time alcohol concentration in the ethanol fermentation tank is provided, and the method produces 1mol CO from the essence of alcoholic fermentation in the time of according to generation 1mol alcohol
2Principle, by the CO that produces in the acid-base neutralization reaction assay fermentation
2Amount calculate the amount of alcohol, the carbon dioxide that produces of preventing from fermenting ensures normally carrying out of sweat at the pH value of solution step-down stopping fermentation that the fermentation tank accumulation causes, and avoids introducing chromatographic equipment or CO
2The equipment expense that detector brings, simple and easy efficiently the real time measure fermentation tank alcohol concentration.
Embodiment
Further specify technical solution of the present invention below in conjunction with specific embodiment, these embodiment can not be interpreted as it is restriction to technical scheme.The high-efficient liquid phase chromatogram condition that uses among the embodiment is: shodex sugar sp0810 chromatographic column, and mobile phase: water, flow velocity 0.5ml/min, 85 ℃ of column temperatures, detecting device adopts the shodexR1-201H differential refraction detector.
Embodiment 1: adopt 7L fermentation tank liquid amount as 5L take 10% glucose as fermentation C source, anaerobic fermentation inserts outlet pipe the Ca that 1L concentration is 5.5mol/L (OH) simultaneously behind the inoculation saccharomyces cerevisiae
2Also stir with magnetic stirrer the bottom of solution; Fermentation 24h, 48h and 96h get respectively the 0.5ml aqueous slkali, dilute 10 times after the hydrochloric acid acid-base titration; Calculate mutually alcohol concentration from fermentation tank sampling feed liquor respectively when alcohol concentration is calculated, the results are shown in Table 1.
Embodiment 2: adopt 7L fermentation tank liquid amount as 5L as the C source take 20% preprocessing straw through 20kg steam pressurize processing 20min, add the cellulosic cellulase of 15u/g, anaerobic fermentation inserts outlet pipe the Na that 1L concentration is 5mol/L (OH) simultaneously behind the inoculation saccharomyces cerevisiae
2Also stir with magnetic stirrer the bottom of solution; Fermentation 24h, 48h and 96h get the 0.5ml aqueous slkali, dilute 10 times after the phosphoric acid acid-base titration; Carry out alcohol concentration calculating and calculate mutually alcohol concentration from fermentation tank sampling feed liquor respectively simultaneously, the results are shown in Table 2.
Embodiment 3: take 10% through the starch of gelatinization saccharification as the C source, adopting 7L fermentation tank liquid amount is 5L, behind the inoculation saccharomyces cerevisiae anaerobic fermentation simultaneously outlet pipe is inserted 1L concentration be 10mol/L ammonia spirit the bottom and stir with magnetic stirrer; Fermentation 24h, 48h and 96h get the 0.5ml aqueous slkali, dilute 10 times after the carbonic acid acid-base titration; Carry out alcohol concentration calculating and calculate mutually alcohol concentration from fermentation tank sampling feed liquor respectively simultaneously, the results are shown in Table 3.
Can find out that by above embodiment detection method of the present invention can reflect that the alcohol concentration in the sweat changes, real-time alcohol concentration suits to be used for measuring.
The invention discloses a kind of easy method that detects in real time alcohol concentration in the ethanol fermentation tank, comprise the CO that produces with in the aqueous slkali of preparing concentration known and the sweat
2React; In real time in the testing process, get the alkali lye that neutralization reaction occurs and carry out titration by the acid of concentration known, be titrated to pH=7, thereby calculate the concentration that alkali lye after the neutralization reaction occurs, and then calculate the molar weight of the alkali that neutralization reaction occurs, finally calculate CO
2Molar weight multiply by again 46 amounts that are alcohol, be alcohol concentration divided by fermentating liquid volume again.The method has been avoided detecting and has been used the expensive equipment such as chromatogram, carbon dioxide detector in the alcohol process, is suitable for especially the small-sized fermentation tank alcoholic fermentation and uses.
Claims (5)
1. the method for fermentation liquor alcohol concentration in the easy the real time measure alcoholic fermentation process is characterized in that: utilize that every generation 1mol alcohol produces 1mol CO simultaneously in the alcoholic fermentation process
2Principle, by CO in the real time measure sweat
2Generation measure alcohol concentration in the fermentation liquor; Concrete assay method may further comprise the steps:
1) according to the throwing amount in C source in the sweat to the theoretical CO that can produce in the sweat
2Molar weight is calculated;
2) theory according to step 1) calculating gained produces CO
2The excessive alkali lye of molar weight configuration guarantee fully and the carbon dioxide generation neutralization reaction that produces, its OH
-Concentration is known volumetric molar concentration C
1, volume is V
1Rise;
3) insert gas exhaust duct in the alkali lye and stirring in the alcoholic fermentation process, guarantee the CO that produces
2Can be in time and the abundant mixing of alkali lye and neutralization reaction occurs;
Volume aspirated V when 4) detecting in real time
2The alkali lye with carbon dioxide generation neutralization reaction that rises, and use known H
+Volumetric molar concentration C
3Acidometric titration to pH=7, read acid and use volume V
3Rise;
5) alcohol volumetric molar concentration C in the sweat
0Calculating be to calculate gained by following formula:
, it is long-pending that wherein V is upgraded to fermentation cylinder for fermentation liquid.
2. the method for fermentation liquor alcohol concentration in the easy the real time measure alcoholic fermentation process according to claim 1, it is characterized in that: the final mass concentration g/L of alcohol is the volumetric molar concentration C of step 5) gained in the fermentation liquor
0Multiply by 46.
3. the method for fermentation liquor alcohol concentration in the easy the real time measure alcoholic fermentation process according to claim 1 is characterized in that: the C source described in the step 1) refers to one or more potpourri of wheat, cassava, cornstarch class material or agricultural crop straw, careless class, forest biomass fiber raw material.
4. the method for fermentation liquor alcohol concentration in the easy the real time measure alcoholic fermentation process according to claim 1 is characterized in that: step 2) described in alkali refer to one or more potpourris in potassium hydroxide, NaOH, ammoniacal liquor, the calcium hydroxide.
5. the method for fermentation liquor alcohol concentration in the easy the real time measure alcoholic fermentation process according to claim 1, it is characterized in that: the acid described in the step 4) refers to one or more potpourris in hydrochloric acid, sulfuric acid, sulphurous acid, nitric acid, phosphoric acid, the carbonic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103913991A CN102901795A (en) | 2012-10-16 | 2012-10-16 | Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012103913991A CN102901795A (en) | 2012-10-16 | 2012-10-16 | Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102901795A true CN102901795A (en) | 2013-01-30 |
Family
ID=47574146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012103913991A Pending CN102901795A (en) | 2012-10-16 | 2012-10-16 | Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102901795A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628552A (en) * | 2015-12-10 | 2016-06-01 | 广州国睿科学仪器有限公司 | Fermentation detection method and device |
| CN109521154A (en) * | 2018-09-25 | 2019-03-26 | 齐鲁工业大学 | A kind of biomass ferment alcohol concentration detecting device and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08240585A (en) * | 1995-03-03 | 1996-09-17 | Yanmar Agricult Equip Co Ltd | Method and device for detection of deterioration of stocked grain |
| JPH1075766A (en) * | 1996-09-05 | 1998-03-24 | Nissin Electric Co Ltd | Fuzzy control in sake brewing |
| CN1525166A (en) * | 2003-02-26 | 2004-09-01 | 中国科学院沈阳应用生态研究所 | Soil ground respiration determination method and device thereof |
| CN102401823A (en) * | 2010-09-09 | 2012-04-04 | 上海海帝园艺有限公司 | Method for evaluating grease biodegradation |
-
2012
- 2012-10-16 CN CN2012103913991A patent/CN102901795A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08240585A (en) * | 1995-03-03 | 1996-09-17 | Yanmar Agricult Equip Co Ltd | Method and device for detection of deterioration of stocked grain |
| JPH1075766A (en) * | 1996-09-05 | 1998-03-24 | Nissin Electric Co Ltd | Fuzzy control in sake brewing |
| CN1525166A (en) * | 2003-02-26 | 2004-09-01 | 中国科学院沈阳应用生态研究所 | Soil ground respiration determination method and device thereof |
| CN102401823A (en) * | 2010-09-09 | 2012-04-04 | 上海海帝园艺有限公司 | Method for evaluating grease biodegradation |
Non-Patent Citations (1)
| Title |
|---|
| BRYCE RANKINE: "《酿造优质葡萄酒》", 30 April 2008, 中国农业大学出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628552A (en) * | 2015-12-10 | 2016-06-01 | 广州国睿科学仪器有限公司 | Fermentation detection method and device |
| CN105628552B (en) * | 2015-12-10 | 2019-12-13 | 广州国睿科学仪器有限公司 | fermentation detection method and detection device |
| CN109521154A (en) * | 2018-09-25 | 2019-03-26 | 齐鲁工业大学 | A kind of biomass ferment alcohol concentration detecting device and method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202830028U (en) | Measurable experimental device for preparing biogas via anaerobic fermentation | |
| CN102980865A (en) | Measurement method for seawater total nitrogen content | |
| CN103076321B (en) | A kind of Continuous Flow Analysis instrument detects the method for formaldehyde in smoke aqueous gel | |
| CN101995400A (en) | Surface enhanced Raman spectroscopy detection method of bisphenol A | |
| CN103743689B (en) | A kind of cozymase NAD or NADH reagent box for detecting content | |
| CN101348816B (en) | Use of low temperature boiling technology in alcohol production | |
| CN103776954A (en) | Device for determining soil breathing rate in laboratory and method for determining soil breathing rate | |
| CN102901795A (en) | Simple method for determining alcohol concentration of fermentation broth in real time during alcoholic fermentation | |
| CN101498713B (en) | Blood-gas reaction monitoring and control device | |
| CN101458241A (en) | Experimental method for detecting reaction speed of carbonate rock and hydrochloric acid | |
| CN202830039U (en) | Improved experimental device for preparing biogas via anaerobic fermentation | |
| CN103728396B (en) | Method for measuring specific methanogenic activity of sludge | |
| Gyalai-Korpos et al. | Evaluation of an online fermentation monitoring system | |
| CN101798603B (en) | Hydrolysis method of lignocellulosic biomass | |
| CN201429441Y (en) | Aluminium powder-evolved gas measurement apparatus | |
| CN107604041B (en) | The measuring method of methoxyl content in a kind of crudefiber crop pectin | |
| CN105784701A (en) | Testing system of nitrogen content of nitrocotton and detecting method of nitrogen content | |
| CN101858845B (en) | Direct-reading osmotic pressure tester and osmotic pressure testing method | |
| CN101799425A (en) | Portable test method of acid value of lipid | |
| CN203414454U (en) | On-line alkali concentration detection equipment | |
| CN202814754U (en) | Circulating sampling system of reaction still | |
| CN104655773A (en) | Method for determining acetic acid content in biomass preprocessing fluid | |
| CN204116322U (en) | A kind of slag has the hot sulks Body components on-Line Monitor Device of pressure | |
| CN105241878B (en) | A kind of aldehydes rapidly measuring device part based on capillarity and Nano Silver recognition principle and its preparation method and application | |
| CN101788462B (en) | Single-channel enzyme activity automatic detecting device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130130 |