CN105617364A

CN105617364A - Multielement nano compound wound care composition and preparing method thereof

Info

Publication number: CN105617364A
Application number: CN201511023419.XA
Authority: CN
Inventors: 潘华; 任艳芳; 李建会
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-12-29
Filing date: 2015-12-29
Publication date: 2016-06-01

Abstract

The invention discloses a multielement nano compound wound care composition and a preparing method thereof. The multielement nano compound wound care composition is prepared from the following components: rheum forrestii, Elaeagnus loureirii Champ., Lycogala epidendrum, Desmodium sinuatum Blume ex Baker, Barnyard grass roots, Eurya aurea (H. Leiv.) Hu & L. K. Ling, Cudrania cochinchinensis Kudo et Masam, Hypserpa nitida, grape seeds, herba leibnitziae anandriae, Anthriscus sylvestris leaf, Aristolochia kwangsiensis Chun et How [A. shukangii Chun et How], Pterocarpus indicus, callicarpa nudiflora, Eupolyphaga sinensis Walker nanopowder, hairyflower actinidia root and leaf nanopowder, spirulina extract, herba artemisiae scopariae extract, decavitamin, lycopene and bromelain. The multielement nano compound wound care composition is mainly used for nursing scars formed after burning scaling, wound or postoperation wound healing, is obvious in hemostasis inflammation-diminishing antibiosis and scar removing treating effects, has characteristics of reasonable compatibility of components, fast effect and low cost, can fast stop bleeding of a wound and has durable inflammation-diminishing and degerming actions and can fade the scars of the wound.

Description

A kind of polynary nanometer compound wound care composition and its preparation method

Technical field

The present invention relates to medical art, specifically relate to a kind of polynary nanometer compound wound care composition and its preparation method.

Background technology

Wound refers to the injured place broken, and refers to the skin of people or other animals, muscle, mucous membrane etc. more, and the degree that wound itself pollutes and later whether infection have very big relation; The wound of current Post operation, darker wound, generally all need to be wound around gauze etc., and such wound inside also comparatively easy breed bacteria, causes inflammation so that wound festers obstinate; After wound healing can scar, the cicatrization time more than two months after, want thoroughly to remove very difficult; The scar area that wound after particularly scalding stays is relatively big, and is difficult to desalination elimination.

For the process of the surface of a wound, skin wound is treated by clinical generally employing antibiotic medicine or some antiphlogistic drugs, though can protect from infection by microbiotic, antiphlogistic drug can suppress the inflammation of the surface of a wound, but wound still to be relied on the tissue of body self to repair, and microbiotic and antiphlogistic drug can delay the healing of wound. Wound healing is slow, brings much inconvenience can to the life of people, increase the misery of patient, but also add the economical load of patient.

Summary of the invention

The technical problem that the present invention solves is to provide a kind of polynary nanometer compound wound care composition and its preparation method, this polynary nanometer compound wound care composition be mainly used in nursing burn, scald, scar that wound or Post operation wound healing are formed, hemostasis, anti-inflammatory, antibacterial, scar of dispelling is evident in efficacy.

The technical scheme of the present invention is: a kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 42��70 parts, paving mountain swallow 40��65 parts, sebaceous cyst bacterium 40��60 parts, ox bar 37��55 parts, mouth, Root of Barnyardgrass 36��50 parts, gold leaf Eurya plant 35��50 parts, Root of Tricuspid Cudrania 32��48 parts, Herba Hypserpae Nitidae 30��45 parts, Semen Vitis viniferae 10��16 parts, Herba Leibnitziae 9��15 parts, 9��13 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 8��12 parts, 6��10 parts, red sandalwood, callicarpa nudiflora 5��9 parts, worm nano powder 3��6 parts, Root of Hairyflower Actinidia nano powder 3��5 parts, spirulina extract 1��3 part, Herba Artemisiae Scopariae extract 1��2 part, vitamin complex 0.02��0.1 part, Lyeopene 0.01��0.04 part, bromeline 0.1��0.2 part.

Further, a kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 30��58 parts, paving mountain swallow 45��60 parts, sebaceous cyst bacterium 45��55 parts, ox bar 42��50 parts, mouth, Root of Barnyardgrass 40��46 parts, gold leaf Eurya plant 38��47 parts, Root of Tricuspid Cudrania 36��44 parts, Herba Hypserpae Nitidae 32��43 parts, Semen Vitis viniferae 11��15 parts, Herba Leibnitziae 10��14 parts, 10��12 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 9��11 parts, 7��9 parts, red sandalwood, callicarpa nudiflora 6��8 parts, worm nano powder 4��5 parts, Root of Hairyflower Actinidia nano powder 3.5��4.5 parts, spirulina extract 1.5��2.5 parts, Herba Artemisiae Scopariae extract 1.2��1.8 parts, vitamin complex 0.04��0.08 part, Lyeopene 0.02��0.03 part, bromeline 0.13��0.17 part.

Further, described a kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 56 parts, paving mountain swallow 52.5 parts, sebaceous cyst bacterium 50 parts, ox bar 46 parts, mouth, Root of Barnyardgrass 43 parts, gold leaf Eurya plant 42.5 parts, Root of Tricuspid Cudrania 40 parts, Herba Hypserpae Nitidae 37.5 parts, Semen Vitis viniferae 13 parts, Herba Leibnitziae 12 parts, 11 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 10 parts, 8 parts, red sandalwood, callicarpa nudiflora 7 parts, worm nano powder 4.5 parts, Root of Hairyflower Actinidia nano powder 4 parts, spirulina extract 2 parts, Herba Artemisiae Scopariae extract 1.5 parts, vitamin complex 0.06 part, Lyeopene 0.025 part, bromeline 0.15 part.

Described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

Further, the preparation method of described spirulina extract is: spirulina adds 2��and the water soaking 3��10h of 5 times amount, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, add the distilled water relative to spirulina powder weight 10��20 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain, it is the ethanol of 90% to the mass concentration adding 1.3��1.5 times amount in filtrate, after mixing, 0��3 DEG C leaves standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, namely spirulina extract is obtained.

Further, the preparation method of described Herba Artemisiae Scopariae extract is: get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 3��6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, it is placed in whizzer 1600��3000r/min centrifugal 5��8 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.86��1.04 at 80 DEG C, and spraying dry makes powder, cross 200 order sieves, obtain Herba Artemisiae Scopariae extract.

Further, the preparation method of described worm nano powder and Root of Hairyflower Actinidia nano powder is: worm dry product and Root of Hairyflower Actinidia dry product are placed in respectively in dryer, at 50��60 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 50��500 ��m again, and then it is ground to 100��300nm through nanon ball-mill, obtain worm nano powder and Root of Hairyflower Actinidia nano powder.

A preparation method for polynary nanometer compound wound care composition, comprises the following steps:

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 10��18 times amount, heating and refluxing extraction 1��2h, filtration obtains filtrate, filtrate concentrate to water content be 10��30%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 3��6h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 100��300nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) get spirulina and add 2��the water soaking 3��10h of 5 times amount, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, add the distilled water relative to spirulina powder weight 10��20 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain, it is the ethanol of 90% to the mass concentration adding 1.3��1.5 times amount in filtrate, after mixing, 0��3 DEG C leaves standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, it is ground to 100��300nm again with nanon ball-mill, obtain spirulina extract, stand-by,

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 3��6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 1600��3000r/min centrifugal 5��8 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.86��1.04 at 80 DEG C, spraying dry makes powder, it is ground to 100��300nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 50��60 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 50��500 ��m again, and then it is ground to 100��300nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(5) vitamin complex, Lyeopene, bromeline, spirulina extract, Herba Artemisiae Scopariae extract, worm nano powder, Root of Hairyflower Actinidia nano powder is taken by proportioning, nanometer extract powder obtained with step (1) is uniformly mixed, and namely obtains described polynary nanometer compound wound care composition.

Further, described polynary nanometer compound wound care composition is prepared into gelifying agent.

Further, the preparation method of described gelifying agent is:

(5) vitamin complex, Lyeopene, bromeline, spirulina extract, Herba Artemisiae Scopariae extract, worm nano powder, Root of Hairyflower Actinidia nano powder is taken by proportioning, nanometer extract powder obtained with step (1) is uniformly mixed, and namely obtains described polynary nanometer compound wound care composition;

(6) 160��200 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 0.7��1.5 part of laurocapram again, being stirred into gel, sterilization, get product gelifying agent.

Compared with prior art, the invention has the beneficial effects as follows:

(1) in the present invention's medicinal material used, husky seven, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora have heat clearing and inflammation relieving, hemostasia and promoting granulation, expelling pus and promoting granulation, the effect of promoting blood circulation to remove blood stasis, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, have antibacterial preferably, antiviral and stop blooding effect, effectively wound can be stopped blooding, and eliminate the bacterium on wound, wherein Fruit of Kwangsi Dutchmanspipe, red sandalwood also has good analgesic effect, can also the pain of reduction of patient, the main component of Semen Vitis viniferae is pycnogenols, there is the effect of anti-oxidant, anti-inflammatory preferably, whitening, scar of dispelling, can effectively desalinate the color of wound scar, and coordinate vitamin A, vitamins C, vitamin P to use, considerably increase whitening and the effect of scar of dispelling, callicarpa nudiflora, Lyeopene, bromeline, have good anti-inflammatory effects, it is possible to effectively avoids wound inflammation, and wherein Lyeopene also has effect of subsiding a swelling preferably, it is possible to eliminates the redness of wound, antibacterial, antipruritic, the anti-infection effect that can strengthen this medicine is used together by spirulina extract, Herba Artemisiae Scopariae extract, worm nano powder, Root of Hairyflower Actinidia nano powder.

(2) the polynary nanometer compound wound care composition compatibility of the present invention is reasonable, it is possible to while to wound quick-acting haemostatic powder, has lasting anti-inflammatory, degerming effect, and can desalinate the scar of wound, also have instant effect, feature that cost is low.

Embodiment

Embodiment 1:

A kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 42 parts, paving mountain swallow 40 parts, sebaceous cyst bacterium 40 parts, ox bar 37 parts, mouth, Root of Barnyardgrass 36 parts, gold leaf Eurya plant 35 parts, Root of Tricuspid Cudrania 32 parts, Herba Hypserpae Nitidae 30 parts, Semen Vitis viniferae 10 parts, Herba Leibnitziae 9 parts, 9 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 8 parts, 6 parts, red sandalwood, callicarpa nudiflora 5 parts, worm nano powder 3 parts, Root of Hairyflower Actinidia nano powder 3 parts, spirulina extract 1 part, Herba Artemisiae Scopariae extract 1 part, vitamin complex 0.02 part, Lyeopene 0.01 part, bromeline 0.1 part. Described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

This polynary nanometer compound wound care composition is prepared into gelifying agent, and preparation method is:

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 10 times amount, heating and refluxing extraction 1h, filtration obtains filtrate, filtrate concentrate to water content be 10%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 3h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 100nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) the water soaking 3h of the spirulina times amount that adds 2 is got, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, adding the distilled water relative to spirulina powder weight 10 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain is the ethanol of 90% to the mass concentration adding 1.3 times amount in filtrate, after mixing, 0 DEG C leaves standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, then it is ground to 100nm with nanon ball-mill, obtain spirulina extract, stand-by;

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 3��6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 1600r/min centrifugal 5 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.86 at 80 DEG C, spraying dry makes powder, it is ground to 100nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 50 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 50 ��m again, and then it is ground to 100nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(6) 160 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 0.7 part of laurocapram again, being stirred into gel, sterilization, get product gelifying agent.

Embodiment 2:

A kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 30 parts, paving mountain swallow 45 parts, sebaceous cyst bacterium 45 parts, ox bar 42 parts, mouth, Root of Barnyardgrass 40 parts, gold leaf Eurya plant 38 parts, Root of Tricuspid Cudrania 36 parts, Herba Hypserpae Nitidae 32 parts, Semen Vitis viniferae 11 parts, Herba Leibnitziae 10 parts, 10 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 9 parts, 7 parts, red sandalwood, callicarpa nudiflora 6 parts, worm nano powder 4 parts, Root of Hairyflower Actinidia nano powder 3.5 parts, spirulina extract 1.5 parts, Herba Artemisiae Scopariae extract 1.2 parts, vitamin complex 0.04 part, Lyeopene 0.02 part, bromeline 0.13 part. Described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 12 times amount, heating and refluxing extraction 1.2h, filtration obtains filtrate, filtrate concentrate to water content be 15%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 4h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 100nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) the water soaking 5h of the spirulina times amount that adds 3 is got, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, adding the distilled water relative to spirulina powder weight 13 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain is the ethanol of 90% to the mass concentration adding 1.3 times amount in filtrate, after mixing, 1 DEG C leaves standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, then it is ground to 150nm with nanon ball-mill, obtain spirulina extract, stand-by;

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 3 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 2000r/min centrifugal 6 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.9 at 80 DEG C, spraying dry makes powder, it is ground to 150nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 52 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 100 ��m again, and then it is ground to 150nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(6) 170 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 0.9 part of laurocapram again, being stirred into gel, sterilization, get product gelifying agent.

Embodiment 3:

A kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 56 parts, paving mountain swallow 52.5 parts, sebaceous cyst bacterium 50 parts, ox bar 46 parts, mouth, Root of Barnyardgrass 43 parts, gold leaf Eurya plant 42.5 parts, Root of Tricuspid Cudrania 40 parts, Herba Hypserpae Nitidae 37.5 parts, Semen Vitis viniferae 13 parts, Herba Leibnitziae 12 parts, 11 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 10 parts, 8 parts, red sandalwood, callicarpa nudiflora 7 parts, worm nano powder 4.5 parts, Root of Hairyflower Actinidia nano powder 4 parts, spirulina extract 2 parts, Herba Artemisiae Scopariae extract 1.5 parts, vitamin complex 0.06 part, Lyeopene 0.025 part, bromeline 0.15 part. described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 14 times amount, heating and refluxing extraction 1.5h, filtration obtains filtrate, filtrate concentrate to water content be 20%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 3��6h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 200nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) the water soaking 6.5h of the spirulina times amount that adds 3.5 is got, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, add the distilled water relative to spirulina powder weight 15 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain, it is the ethanol of 90% to the mass concentration adding 1.4 times amount in filtrate, after mixing, 1.5 DEG C leave standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, it is ground to 200nm again with nanon ball-mill, obtain spirulina extract, stand-by,

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 3��6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 2300r/min centrifugal 6.5 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.95 at 80 DEG C, spraying dry makes powder, it is ground to 200nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 55 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 275 ��m again, and then it is ground to 200nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(6) 180 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 1.1 parts of laurocaprams again, being stirred into gel, sterilization, get product gelifying agent.

Embodiment 4:

A kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 58 parts, paving mountain swallow 60 parts, sebaceous cyst bacterium 55 parts, ox bar 50 parts, mouth, Root of Barnyardgrass 46 parts, gold leaf Eurya plant 47 parts, Root of Tricuspid Cudrania 44 parts, Herba Hypserpae Nitidae 43 parts, Semen Vitis viniferae 15 parts, Herba Leibnitziae 14 parts, 12 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 11 parts, 9 parts, red sandalwood, callicarpa nudiflora 8 parts, worm nano powder 5 parts, Root of Hairyflower Actinidia nano powder 4.5 parts, spirulina extract 2.5 parts, Herba Artemisiae Scopariae extract 1.8 parts, vitamin complex 0.08 part, Lyeopene 0.03 part, bromeline 0.17 part. Described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 16 times amount, heating and refluxing extraction 1.8h, filtration obtains filtrate, filtrate concentrate to water content be 25%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 5h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 300nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) the water soaking 8h of the spirulina times amount that adds 4 is got, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, adding the distilled water relative to spirulina powder weight 17 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain is the ethanol of 90% to the mass concentration adding 1.5 times amount in filtrate, after mixing, 2 DEG C leave standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, then it is ground to 250nm with nanon ball-mill, obtain spirulina extract, stand-by;

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 2600r/min centrifugal 7 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 1.0 at 80 DEG C, spraying dry makes powder, it is ground to 250nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 58 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 400 ��m again, and then it is ground to 250nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(6) 190 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 1.3 parts of laurocaprams again, being stirred into gel, sterilization, get product gelifying agent.

Embodiment 5:

A kind of polynary nanometer compound wound care composition, organize part meter by weight, comprise following composition: husky 7 70 parts, paving mountain swallow 65 parts, sebaceous cyst bacterium 60 parts, ox bar 55 parts, mouth, Root of Barnyardgrass 50 parts, gold leaf Eurya plant 50 parts, Root of Tricuspid Cudrania 48 parts, Herba Hypserpae Nitidae 45 parts, Semen Vitis viniferae 16 parts, Herba Leibnitziae 15 parts, 13 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 12 parts, 10 parts, red sandalwood, callicarpa nudiflora 9 parts, worm nano powder 6 parts, Root of Hairyflower Actinidia nano powder 5 parts, spirulina extract 3 parts, Herba Artemisiae Scopariae extract 2 parts, vitamin complex 0.1 part, Lyeopene 0.04 part, bromeline 0.2 part. Described vitamin complex is formulated according to the weight ratio of 1:3:2 by vitamin A, vitamins C, vitamin P, it is possible to promote wound healing.

(1) husky seven are taken by proportioning, paving mountain swallow, sebaceous cyst bacterium, ox Ba Zui, Root of Barnyardgrass, gold leaf Eurya plant, Root of Tricuspid Cudrania, Herba Hypserpae Nitidae, Semen Vitis viniferae, Herba Leibnitziae, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe, red sandalwood, callicarpa nudiflora, clean, dry, and said medicine was pulverized respectively 1200 order sieves, obtain Chinese medicine powder mix, it is placed in multi-function extractor, add the pure water of 18 times amount, heating and refluxing extraction 2h, filtration obtains filtrate, filtrate concentrate to water content be 30%, the ethanol of mass concentration 50% is added by the volume ratio of 1:3, stir evenly, leave standstill 6h, get supernatant liquor, decompression recycling ethanol, it is condensed into medicinal extract, spraying dry makes extract powder, it is ground to 300nm again with nanon ball-mill, obtain a nanometer extract powder, stand-by,

(2) the water soaking 10h of the spirulina times amount that adds 5 is got, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, adding the distilled water relative to spirulina powder weight 20 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain is the ethanol of 90% to the mass concentration adding 1.5 times amount in filtrate, after mixing, 3 DEG C leave standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, then it is ground to 300nm with nanon ball-mill, obtain spirulina extract, stand-by;

(3) get oriental wormwood pulverize after cross 40 orders sieve, then add relative to the mass concentration of oriental wormwood powder 6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filter, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, being placed in whizzer 3000r/min centrifugal 8 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 1.04 at 80 DEG C, spraying dry makes powder, it is ground to 300nm with nanon ball-mill again, obtains Herba Artemisiae Scopariae extract, stand-by;

(4) worm dry product is placed in dryer, at 60 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, pulverize into, with supper micron mill, the fine powder that particle diameter is 500 ��m again, and then it is ground to 300nm through nanon ball-mill, obtain worm nano powder, Root of Hairyflower Actinidia dry product is prepared Root of Hairyflower Actinidia nano powder according to same technique, for subsequent use;

(6) 200 parts of carbomers being added suitable quantity of water swelling, adjust ph to 7.0, polynary nanometer compound wound care composition step (5) obtained adds wherein, add 1.5 parts of laurocaprams again, being stirred into gel, sterilization, get product gelifying agent.

Clinical application typical case:

1, Zhu, female, 10 years old, scalding area was bigger, skin surface has more bubble, surface irregularity, uses care composition of the present invention to be applied in affected part after treatment, it may also be useful to after three courses for the treatment of, wound healing is formed a scab, and after continuing to use four courses for the treatment of, the scar after coming off of forming a scab be can't see substantially.

2, fourth so-and-so, female, 25 years old, carried out widening the view angle operation, and after art after using care composition of the present invention to be applied in three courses for the treatment of of wound, wound healing is formed a scab, and incrustation come off after substantially there is no scar.

3, king so-and-so, female, 34 years old, forehead is injured, and wound relatively big relatively dark needs is sewed up, and uses care composition of the present invention to be applied in incrustation after two courses for the treatment of of affected part after stitching, after continuing to use a course for the treatment of, incrustation come off after scar paler colour, continue to use after two courses for the treatment of, scar is not seen substantially.

Last it is noted that above embodiment is only in order to illustrate the technical scheme of the present invention, it is not intended to limit; Although with reference to previous embodiment to invention has been detailed description, it will be understood by those within the art that: the technical scheme described in previous embodiment still can be modified by it, or wherein part technology feature is carried out equivalent replacement; And these amendments or replacement, do not make the spirit and scope of the essence disengaging embodiment of the present invention technical scheme of appropriate technical solution.

Claims

1. a polynary nanometer compound wound care composition, it is characterized in that, organize part meter by weight, comprise following composition: husky 7 42��70 parts, paving mountain swallow 40��65 parts, sebaceous cyst bacterium 40��60 parts, ox bar 37��55 parts, mouth, Root of Barnyardgrass 36��50 parts, gold leaf Eurya plant 35��50 parts, Root of Tricuspid Cudrania 32��48 parts, Herba Hypserpae Nitidae 30��45 parts, Semen Vitis viniferae 10��16 parts, Herba Leibnitziae 9��15 parts, 9��13 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 8��12 parts, 6��10 parts, red sandalwood, callicarpa nudiflora 5��9 parts, worm nano powder 3��6 parts, Root of Hairyflower Actinidia nano powder 3��5 parts, spirulina extract 1��3 part, Herba Artemisiae Scopariae extract 1��2 part, vitamin complex 0.02��0.1 part, Lyeopene 0.01��0.04 part, bromeline 0.1��0.2 part.

2. a kind of polynary nanometer compound wound care composition as claimed in claim 1, it is characterized in that, organize part meter by weight, comprise following composition: husky 7 30��58 parts, paving mountain swallow 45��60 parts, sebaceous cyst bacterium 45��55 parts, ox bar 42��50 parts, mouth, Root of Barnyardgrass 40��46 parts, gold leaf Eurya plant 38��47 parts, Root of Tricuspid Cudrania 36��44 parts, Herba Hypserpae Nitidae 32��43 parts, Semen Vitis viniferae 11��15 parts, Herba Leibnitziae 10��14 parts, 10��12 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 9��11 parts, 7��9 parts, red sandalwood, callicarpa nudiflora 6��8 parts, worm nano powder 4��5 parts, Root of Hairyflower Actinidia nano powder 3.5��4.5 parts, spirulina extract 1.5��2.5 parts, Herba Artemisiae Scopariae extract 1.2��1.8 parts, vitamin complex 0.04��0.08 part, Lyeopene 0.02��0.03 part, bromeline 0.13��0.17 part.

3. a kind of polynary nanometer compound wound care composition as claimed in claim 1, it is characterized in that, organize part meter by weight, comprise following composition: husky 7 56 parts, paving mountain swallow 52.5 parts, sebaceous cyst bacterium 50 parts, ox bar 46 parts, mouth, Root of Barnyardgrass 43 parts, gold leaf Eurya plant 42.5 parts, Root of Tricuspid Cudrania 40 parts, Herba Hypserpae Nitidae 37.5 parts, Semen Vitis viniferae 13 parts, Herba Leibnitziae 12 parts, 11 parts, wild chervil leaf, Fruit of Kwangsi Dutchmanspipe 10 parts, 8 parts, red sandalwood, callicarpa nudiflora 7 parts, worm nano powder 4.5 parts, Root of Hairyflower Actinidia nano powder 4 parts, spirulina extract 2 parts, Herba Artemisiae Scopariae extract 1.5 parts, vitamin complex 0.06 part, Lyeopene 0.025 part, bromeline 0.15 part.

4. a kind of polynary nanometer compound wound care composition as claimed in claim 1, it is characterized in that, the preparation method of described spirulina extract is: spirulina adds 2��and the water soaking 3��10h of 5 times amount, pulverize after oven dry, cross 40 order sieves, obtain spirulina powder, add the distilled water relative to spirulina powder weight 10��20 times, carry out microwave treatment, arranging microwave frequency is 560-700MHz, treatment time is 30min, the reaction solution cold filtration that will obtain, it is the ethanol of 90% to the mass concentration adding 1.3��1.5 times amount in filtrate, after mixing, 0��3 DEG C leaves standstill refrigeration 24h, centrifugation, the throw out spraying dry that will obtain, namely spirulina extract is obtained.

5. a kind of polynary nanometer compound wound care composition as claimed in claim 1, it is characterized in that, the preparation method of described Herba Artemisiae Scopariae extract is: gets and crosses 40 order sieves after oriental wormwood is pulverized, then add relative to the mass concentration of oriental wormwood powder 3��6 times of weight be 70% aqueous acetone solution refluxing extraction 2h, filtering, filtrate is for subsequent use; Filter residue is the aqueous acetone solution refluxing extraction 1h of 70% again by mass concentration, filter, merge the filtrate of twice, it is placed in whizzer 1600��3000r/min centrifugal 5��8 minutes, then by the supernatant liquor silica gel column chromatography of gained, gained chromatographic solution is evaporated to the thick cream that relative density is 0.86��1.04 at 80 DEG C, and spraying dry makes powder, cross 200 order sieves, obtain Herba Artemisiae Scopariae extract.

6. a kind of polynary nanometer compound wound care composition as claimed in claim 1, it is characterized in that, the preparation method of described worm nano powder and Root of Hairyflower Actinidia nano powder is: worm dry product and Root of Hairyflower Actinidia dry product are placed in respectively in dryer, at 50��60 DEG C of oven drying at low temperatures to water content lower than 5%, taking-up is cooled to room temperature, first it is crushed to 50 orders with pulverizer, the fine powder that particle diameter is 50��500 ��m is pulverized into again with supper micron mill, and then it is ground to 100��300nm through nanon ball-mill, obtain worm nano powder and Root of Hairyflower Actinidia nano powder.

7. prepare the method for the polynary nanometer compound wound care composition as described in claim 1��6 for one kind, it is characterised in that, comprise the following steps:

8. prepare the method for polynary nanometer compound wound care composition as claimed in claim 7 for one kind, it is characterised in that, described polynary nanometer compound wound care composition is prepared into gelifying agent.

9. prepare the method for polynary nanometer compound wound care composition as claimed in claim 7 for one kind, it is characterised in that, the preparation method of described gelifying agent is: