CN105614156A

CN105614156A - Feed and additive for improving shrimp production performance

Info

Publication number: CN105614156A
Application number: CN201510972376.3A
Authority: CN
Inventors: 许梅
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-12-22
Filing date: 2015-12-22
Publication date: 2016-06-01

Abstract

The invention provides a feed additive for improving shrimp production performance. The feed additive for improving shrimp production performance comprises the following raw materials: Chinese herbal medicine feed additives capable of improving shrimp production performance, zinc methionine, ferrous methionine and glutamine. Fed on a feed containing the feed additive, the shrimps have better digestion and absorption capacities as well as stress-resistant performances; thus, the shrimps have better growth and development performances and maintain the best immune functions. Moreover, the additive causes no drug residue and drug resistance.

Description

Feedstuff and the additive of performance is produced for improving shrimp

Technical field

The present invention relates to field of feed, particularly relate to a kind of feedstuff and additive producing performance for improving shrimp.

Background technology

Crusta Penaeus seu Panulirus is thin, carapace is little, dressed fish is high, delicious meat, nutritious, extensively cultivate in China. But along with expansion and the aquifer cultivation ecological deterioration of intensive, large-scale cultivation, the disease of prawn is on the rise, and prawn culturing dealer's economic benefit significantly glides. Up to now, it has been found that have more than 20 kind of viral infection shrimps, there is no effectively preventing method, seriously govern the development of shrimp aquaculture industry.

In the last few years, shrimp culture disease was broken out frequently. For the preventing and treating of shrimps disease, traditional method is to use antibiotic etc, can quickly suppress or kill pathogen to a certain extent, effectively controls the generation of disease and spreads. But, in feedstuff, add antibiotic for a long time can cause animal autogenous infection, drug residue, generation Resistant strain, make the problems such as shrimp immunologic function degression. Seek antibiotic succedaneum, be always up the study hotspot of feed industry.

Chinese herbal medicine is natural, environmental protection, noresidue, also there is Anti-bacterium, antiviral, improve immunologic function and improve the effect such as body resistance against diseases, overcome the defect of chemicals and antibiotics, be widely used in culture fishery. Chinese herbal medicine contains the panimmunity active factorses such as abundant organic acid, alkaloid, polysaccharide class, can pass through affect non-specific immune systems, activate and induce the approach such as cytokine profiles raising organism immune power, and there is natural, efficient, low-residual, be not easily caused the advantages such as drug resistance. Feedstuff adds Chinese herbal medicine and can not only improve the resistance against diseases of cultivated animals, also can reach somatotrophic effect by raising cultivated animals digestive enzyme activity simultaneously. Chinese herbal medicine the growth promoting function of aquatic animal is likely to Chinese herbal medicine in containing multiple nutritional components, bioactive substance and promote digesting and assimilating of feedstuff Middle nutrition composition relevant by adjustment gut of shrimp digestive enzyme and the composition of intestinal microbial population.

The present invention provides a kind of Chinese herbal feed additive and the feedstuff comprising this Chinese herbal feed additive, by this feedstuff of feeding, make shrimp have to digest and assimilate preferably and the ability of anti-stress, thus obtain good growth performance and keep the immune function of the best, and it will not produce drug residue and drug resistance.

Summary of the invention

The technical problem to be solved is to provide a kind of feed additive and the feedstuff comprising this feed additive, by this feedstuff of feeding, make shrimp have to digest and assimilate preferably and the ability of anti-stress, thus obtain good growth performance and keep the immune function of the best, and it will not produce drug residue and drug resistance.

For solving above-mentioned technical problem, the present invention provides a kind of feed additive producing performance for improving shrimp, and its raw material includes for improving the shrimp production Chinese herbal feed additive of performance, zinc methionine, ferrous methionine and glutamine.

Wherein, the weight of each raw material respectively Chinese herbal feed additive 1kg, zinc methionine 50g, ferrous methionine 50g and glutamine 75g in described feed additive.

Wherein, the crude drug of described Chinese herbal feed additive includes: Rhizoma Coptidis, Semen Euryales, Herba Houttuyniae, Caulis Spatholobi, Herba Ephedrae, Gaultheria, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati), Herba Hyperici Sampsonii, Herba Stellariae Alsines, Fructus Pistaciae Verae, Fructus Mume, Flos Hibisci Rosae-Sinensis, Herba Apii graveolentis, Herba Eleusines Indicae, Radix Campanumoeae, Pericarpium Citri Reticulatae, Rhizoma Kaempferiae, Caulis Lonicerae, achillea millefolium, the Radix Stemonae, Rabdosia rubescens.

Wherein, the weight respectively Rhizoma Coptidis 50g ~ 60g of each crude drug, Semen Euryales 25g ~ 35g, Herba Houttuyniae 20g ~ 30g, Caulis Spatholobi 25g ~ 35g, Herba Ephedrae 30g ~ 40g, Gaultheria 15g ~ 25g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 10g ~ 20g, Herba Hyperici Sampsonii 20g ~ 30g, Herba Stellariae Alsines 15g ~ 25g, Fructus Pistaciae Verae 20g ~ 30g, Fructus Mume 20g ~ 30g, Flos Hibisci Rosae-Sinensis 15g ~ 25g, Herba Apii graveolentis 20g ~ 30g, Herba Eleusines Indicae 20g ~ 30g, Radix Campanumoeae 25g ~ 35g, Pericarpium Citri Reticulatae 20g ~ 30g, Rhizoma Kaempferiae 20g ~ 30g, Caulis Lonicerae 25g ~ 35g, achillea millefolium 20g ~ 30g, Radix Stemonae 10g ~ 20g and Rabdosia rubescens 20g ~ 30g in described Chinese herbal feed additive.

The preparation method that present invention also offers above-mentioned feed additive, particularly as follows:

The first step, prepares described Chinese herbal feed additive;

Second step, sends into each raw material components in mixer in proportion and is uniformly mixed, it is thus achieved that compound, the compound rotary drum dryer of temperature 180 DEG C��220 DEG C is dried 2��3 minutes, the water content making compound is 5%��10%, prepares semi-finished product, is sieved by semi-finished product, the aperture that first time sieves is 1mm, obtain finished product, filter out the little granule more than 1mm and again pulverize, again sieve, mix with finished product above, it is thus achieved that feed additive.

Wherein, the described first step further particularly as follows:

A walks; Rhizoma Coptidis, Semen Euryales, Caulis Spatholobi, Herba Hyperici Sampsonii, Flos Hibisci Rosae-Sinensis, Radix Campanumoeae, Caulis Lonicerae, achillea millefolium, Rabdosia rubescens are respectively crushed into powder; stir mixing by weight; cross 20 mesh sieves; obtain mixture; adopt water boiling and extraction 1��2 time; for the mixture of every 1kg, water uses 6��8L, extracts 1��1.5 hour every time; sucking filtration; removing filtering residue, merging filtrate, the extracting solution obtained adopts Reduced Pressure Concentration Device at 70 DEG C��80 DEG C; vacuum is 0.09Mpa��0.095Mpa, and solution to be concentrated feed liquor flow velocity is 150��200ml min^-1When, concentrates, and the concentrated extract obtained carries out vacuum drying at 45 DEG C��55 DEG C, pulverizes, obtains the first powder;

B walks, and remaining components is respectively crushed into powder, stirs mixing by weight, cross 20 mesh sieves, it is thus achieved that mixture, add the ethanol that determining alcohol is 60% ~ 80% relative to its quality 5 ~ 8 times, being heated to reflux 2 ~ 4 hours, filter, filtering residue adds the ethanol that determining alcohol is 60% ~ 80% relative to mixture quality 2 ~ 4 times again, it is heated to reflux 2 ~ 4 hours, filters, filtrate is merged, heating concentration removes solvent, obtain and get dry extract, pulverize, obtain the second powder;

C walks, and a powder and b powder is mixed, it is thus achieved that Chinese herbal feed additive.

Present invention also offers a kind of feedstuff producing performance for improving shrimp, its raw material includes fish flour, bean cake, Semen arachidis hypogaeae dregs, meat meal tankage, flour, beer yeast, fish oil, dalcium biphosphate, soybean phospholipid, choline chloride, vitamin C, potassium chloride and feed additive.

Wherein, the weight of each raw material respectively fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg, feed additive 0.05kg in described feedstuff.

The preparation method that present invention also offers above-mentioned feedstuff, particularly as follows:

Each raw material pulverizing crosses mix homogeneously after 60 mesh sieves, makes 1.0mm diameter particle feedstuff, first toasts 2h in 90 DEG C of baking ovens, then drains in air-conditioned room, seals and save backup in-20 DEG C of refrigerators.

Beneficial effects of the present invention:

The present invention provides a kind of feed additive and the feedstuff comprising this feed additive, by this feedstuff of feeding, make shrimp have to digest and assimilate preferably and the ability of anti-stress, thus obtain good growth performance and keep the immune function of the best, and it will not produce drug residue and drug resistance.

Detailed description of the invention

The invention provides a kind of Chinese herbal feed additive for improving shrimp growth performance, its crude drug includes: Rhizoma Coptidis, Semen Euryales, Herba Houttuyniae, Caulis Spatholobi, Herba Ephedrae, Gaultheria, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati), Herba Hyperici Sampsonii, Herba Stellariae Alsines, Fructus Pistaciae Verae, Fructus Mume, Flos Hibisci Rosae-Sinensis, Herba Apii graveolentis, Herba Eleusines Indicae, Radix Campanumoeae, Pericarpium Citri Reticulatae, Rhizoma Kaempferiae, Caulis Lonicerae, achillea millefolium, the Radix Stemonae, Rabdosia rubescens.

Further, described Chinese herbal feed additive is only made up of above-mentioned raw materials medicine.

The weight of each crude drug respectively Rhizoma Coptidis 50g ~ 60g in described Chinese herbal feed additive, Semen Euryales 25g ~ 35g, Herba Houttuyniae 20g ~ 30g, Caulis Spatholobi 25g ~ 35g, Herba Ephedrae 30g ~ 40g, Gaultheria 15g ~ 25g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 10g ~ 20g, Herba Hyperici Sampsonii 20g ~ 30g, Herba Stellariae Alsines 15g ~ 25g, Fructus Pistaciae Verae 20g ~ 30g, Fructus Mume 20g ~ 30g, Flos Hibisci Rosae-Sinensis 15g ~ 25g, Herba Apii graveolentis 20g ~ 30g, Herba Eleusines Indicae 20g ~ 30g, Radix Campanumoeae 25g ~ 35g, Pericarpium Citri Reticulatae 20g ~ 30g, Rhizoma Kaempferiae 20g ~ 30g, Caulis Lonicerae 25g ~ 35g, achillea millefolium 20g ~ 30g, Radix Stemonae 10g ~ 20g and Rabdosia rubescens 20g ~ 30g.

It is preferred that, the weight of each crude drug respectively Rhizoma Coptidis 53g ~ 57g in described Chinese herbal feed additive, Semen Euryales 28g ~ 32g, Herba Houttuyniae 23g ~ 27g, Caulis Spatholobi 28g ~ 32g, Herba Ephedrae 33g ~ 37g, Gaultheria 18g ~ 22g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 13g ~ 17g, Herba Hyperici Sampsonii 23g ~ 27g, Herba Stellariae Alsines 18g ~ 22g, Fructus Pistaciae Verae 23g ~ 27g, Fructus Mume 23g ~ 27g, Flos Hibisci Rosae-Sinensis 18g ~ 22g, Herba Apii graveolentis 23g ~ 27g, Herba Eleusines Indicae 23g ~ 27g, Radix Campanumoeae 28g ~ 32g, Pericarpium Citri Reticulatae 23g ~ 27g, Rhizoma Kaempferiae 23g ~ 27g, Caulis Lonicerae 28g ~ 32g, achillea millefolium 23g ~ 27g, Radix Stemonae 13g ~ 17g and Rabdosia rubescens 23g ~ 27g.

Most preferably, the weight of each crude drug respectively Rhizoma Coptidis 55g, Semen Euryales 30g, Herba Houttuyniae 25g, Caulis Spatholobi 30g, Herba Ephedrae 35g, Gaultheria 20g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 15g, Herba Hyperici Sampsonii 25g, Herba Stellariae Alsines 20g, Fructus Pistaciae Verae 25g, Fructus Mume 25g, Flos Hibisci Rosae-Sinensis 20g, Herba Apii graveolentis 25g, Herba Eleusines Indicae 25g, Radix Campanumoeae 30g, Pericarpium Citri Reticulatae 25g, Rhizoma Kaempferiae 25g, Caulis Lonicerae 30g, achillea millefolium 25g, Radix Stemonae 15g and Rabdosia rubescens 25g in described Chinese herbal feed additive.

The preparation method that the present invention also provides for said herbal medicine feed additive, particularly as follows:

The first step; Rhizoma Coptidis, Semen Euryales, Caulis Spatholobi, Herba Hyperici Sampsonii, Flos Hibisci Rosae-Sinensis, Radix Campanumoeae, Caulis Lonicerae, achillea millefolium, Rabdosia rubescens are respectively crushed into powder; stir mixing by weight; cross 20 mesh sieves; obtain mixture; adopt water boiling and extraction 1��2 time; for the mixture of every 1kg, water uses 6��8L, extracts 1��1.5 hour every time; sucking filtration; removing filtering residue, merging filtrate, the extracting solution obtained adopts Reduced Pressure Concentration Device at 70 DEG C��80 DEG C; vacuum is 0.09Mpa��0.095Mpa, and solution to be concentrated feed liquor flow velocity is 150��200ml min^-1When, concentrates, and the concentrated extract obtained carries out vacuum drying at 45 DEG C��55 DEG C, pulverizes, obtains the first powder;

Second step, is respectively crushed into powder by remaining components, stirs mixing by weight, cross 20 mesh sieves, it is thus achieved that mixture, add the ethanol that determining alcohol is 60% ~ 80% relative to its quality 5 ~ 8 times, being heated to reflux 2 ~ 4 hours, filter, filtering residue adds the ethanol that determining alcohol is 60% ~ 80% relative to mixture quality 2 ~ 4 times again, it is heated to reflux 2 ~ 4 hours, filters, filtrate is merged, heating concentration removes solvent, obtain and get dry extract, pulverize, obtain the second powder;

3rd step, mixes the first powder and the second powder, it is thus achieved that Chinese herbal feed additive.

The present invention also provides for a kind of feed additive producing performance for improving shrimp, and its raw material includes Chinese herbal feed additive, zinc methionine, ferrous methionine and glutamine.

It is preferred that, described feed additive is only made up of above-mentioned raw materials.

The weight of each raw material respectively Chinese herbal feed additive 1kg, zinc methionine 50g, ferrous methionine 50g and glutamine 75g in described feed additive.

Zinc and ferrum are one of trace element affecting body immune system, zinc, ferrum are for immunocyte, particularly the growth of thymocyte cell, growth and maturation have important effect, and have great importance in maintaining human body immune function and preventing pathogenic bacteria, parasitic infestation. Zinc, due to the importance in nutrition and immunologic function, is extensively subject to people's attention. But the zinc in daily ration not necessarily all can be directly utilized by organism. Inorganic zinc element must first combine with suitable organic ligand in gastrointestinal tract, could be utilized by body, and the present invention, by joining in feed additive by zinc methionine and ferrous methionine, improves the immunologic function of shrimp, improves further and produces performance.

Glutamine plays very important effect in maintaining the normal physiological function of animal body, particularly under some stressed conditions, add external source glutamine and can be obviously improved animal intestinal 26S Proteasome Structure and Function integrity, strengthen digestion and absorption function, improve secretory volume and the activity thereof of immunocyte, strengthen the function of whole body immune system.

The preparation method that present invention also offers above-mentioned feed additive, it is specially to send in mixer each raw material components in proportion and is uniformly mixed, obtain compound, the compound rotary drum dryer of temperature 180 DEG C��220 DEG C is dried 2��3 minutes, the water content making compound is 5%��10%, prepare semi-finished product, semi-finished product are sieved, the aperture that first time sieves is 1mm, obtain finished product, filter out the little granule more than 1mm and again pulverize, again sieve, mix with finished product above, it is thus achieved that feed additive.

Each crude drug pharmacology is as follows:

Rhizoma Coptidis: bitter in the mouth, cold in nature, enter the heart, lung, gallbladder, stomach, large intestine channel, have heat clearing and damp drying, eliminating fire and detoxication, the effect of cooling blood for improving eyesight, be usually used in the fire for the treatment of removing pathogen in the heart warp, the dysphoria and insomnia that heart-fire is prosperous, and the high fever coma of epidemic febrile disease, the symptom such as vexed, thirsty.

Semen Euryales: sweet in the mouth, puckery, property is put down. Return spleen, kidney channel. There is supplementing the kidney to control the nocturnal, tonifying spleen and stopping diarrhea, the function of damp eliminating leukorrhagia stopping, cure mainly emission, diarrhea due to hypofunction of the spleen, insufficiency of the spleen nebulousurine, be with inferior card.

Herba Houttuyniae: latin name houttuyniaeherba, acrid in the mouth, cold nature, enters lung, bladder warp, has heat-clearing and toxic substances removing, removing pus and relieving carbuncle, effect of inducing diuresis for treating stranguria syndrome, and for lung abscess vomiting pus, expectorant heat syndrome cough is breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, pyretic toxicity carbuncle sore, and snakeworm wants first-class card.

Caulis Spatholobi: latin name spatholobicaulis, bitter in the mouth, sweet, warm in nature, return liver, kidney channel, there is expansion blood vessel, replenishing and activating blood, regulating menstruation, effect of relaxing muscles and tendons and activating QI and blood in the collateral, curing mainly blood deficiency has the menoxenia of silt, and limbs pain is meciless, and soreness of the waist and knees, pain etc. are demonstrate,proved.

Herba Ephedrae: acrid in the mouth, micro-hardship, warm in nature, enter lung, bladder warp, have and send out cold inducing sweat, depressed lung-energy dispersing, the effect of inducing diuresis to remove edema, be mainly used in the exterior excess syndrome such as fever without perspiration of affection of exogenous wind-cold; Rheumatic arthralgia pain; Pathogenic cold attacking the lung or accumulation of heat in the lung are to cough with asthma QI rising in reverse order, edema, and dysuria etc. is demonstrate,proved.

Gaultheria: for root or the stem and leaf of the white strain tree of Ericaceae Gaultheria plant, acrid in the mouth, puckery, warm in nature, gas is fragrant, has effect of expelling wind and removing dampness, cures mainly rheumatic arthritis, and traumatic injury etc. is demonstrate,proved.

Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati): the flower of Rubiaceae Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) platymiscium Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati), sweet in the mouth, light, cool in nature, there is dissipating blood stasis hemostasis, regulating menstruation, blood pressure lowering, liver heat removing, invigorate blood circulation, effect of pain relieving, cure mainly hypertension, menoxenia, injured bone, skin infection. Root, stem: hemoptysis of pulmonary tuberculosis, stomachache, rheumatic arthritis, traumatic injury etc. is demonstrate,proved.

Herba Hyperici Sampsonii: for the herb of Guttiferae hypericum Herba Hyperici Sampsonii (nontoxic): bitter in the mouth, pungent, cold in nature, return liver, spleen channel, there is removing pathogenic heat from blood and toxic substance from the body, promoting blood flow to regulate menstruation, effect of dispelling wind and removing obstruction in the collateral, cure mainly various hemorrhage, enteritis and dysentery, acute mastitis, carbuncle furunculosis, scalds, venom, menoxenia, dysmenorrhea, leucorrhea, traumatic injury, rheumatic arthralgia, aphtha etc. is demonstrate,proved.

The cards such as Herba Stellariae Alsines: for the herb of Caryophyllaceae Stellaria plant Stellaria alsine Grim., acrid in the mouth, property is put down, and returns lung, spleen channel, has expelling wind and removing dampness, promoting blood circulation and detumescence, effect of removing toxic substances hemostasis, cure mainly flu, dysentery, rheumatic arthralgia, traumatic injury, venom, spit blood, traumatic hemorrhage.

Fructus Pistaciae Verae: for the fruit of Anacardiaceae pistache plant pistachio, acrid in the mouth, warm in nature, puckery, there is the kidney warming, effect of warm spleen, cure mainly waist of suffering from a deficiency of the kidney cold, sexual impotence, the card such as insufficiency of the spleen cold-dysentery.

Fructus Mume: sour in the mouth, property is put down, and returns liver, spleen, lung, large intestine channel, has astringing lung-QI and relieving cough, relieving diarrhea with astringents, hemostasis, the effect promoted the production of body fluid, cures mainly chronic cough more than, chronic diarrhea chronic dysentery, the cards such as hematuria is had blood in stool, metrorrhagia, deficiency-heat excessive thirst, sore and toxic.

Flos Hibisci Rosae-Sinensis: for the flower of Malvaceae Hibiscus plant Zhu Jin, sweet in the mouth, cold in nature, there is lung heat clearing, removing heat from blood, dampness removing, effect of removing toxic substances, cure mainly cough due to lung-heat, hemoptysis, metrorrhagia, leucorrhea, dysentery, reddish and whitish turbid urine, the card such as carbuncle skin infection.

Herba Apii graveolentis: for the whole herb with root of Umbelliferae apium plant Herba Apii graveolentis, sweet in the mouth, hardship pungent, micro-, cool in nature, return liver, stomach, lung meridian, there is suppressing the hyperactive liver, heat clearing away, dispel the wind, dampness removing, hemostasis, effect of removing toxic substances, cures mainly dizziness due to hyperactivity of liver-YANG, headache due to pathogenic wind-heat, cough, jaundice, urine leaching leads to, hematuria, bleeding not during menses, sore swollen toxin etc. is demonstrate,proved.

Herba Eleusines Indicae: for root or the herb of grass family yard grass platymiscium Herba Eleusines Indicae, sweet in the mouth, light, cool in nature, there is heat-clearing and toxic substances removing, dampness removing, effect of cool the blood dissipate blood stasis, curing mainly Sunstroke heating, infantile convulsion, encephalitis b, epidemic encephalitis, jaundice, stranguria; dysuria, dysentery, have blood in stool, urticaria, traumatic injury etc. is demonstrate,proved.

Radix Campanumoeae: latin name radixcampanumoeajavanicae, for the root of campanulaceae leopard platymiscium Campanumoea javanica Yu leopard, sweet in the mouth, property is put down, and returns lung, spleen channel, has invigorating the spleen and benefiting QI, tonifying the lung and stopping cough, effect of stimulating milk secretion, cures mainly labor void internal injury, and the deficiency of vital energy is weak, cardiopalmus, hyperhidrosis, diarrhea due to hypofunction of the spleen, leucorrhea, the cards such as milk is rare, insufficiency of the spleen cough.

Pericarpium Citri Reticulatae: warm in nature, acrid in the mouth, hardship; Return spleen, lung meridian, for diseases such as distension and fullness of the chest and abdomen. Pericarpium Citri tangerinae is pungent dissipates logical temperature, fragrant odour, is longer than and regulates the flow of vital energy, spleen lung can be entered, therefore scattered sluggishness of lung-QI can be gone, again can promoting the circulation of QI to alleviate the stagnation in middle-JIAO, gather around stagnant, chest and diaphragm feeling of fullness and the disease such as stagnation of QI in spleen and stomach, distension and fullness in the abdomen for lung qi, for retention of dampness in middle-JIAO, gastral cavity abdomen painful abdominal mass is swollen, have loose bowels in loose stool, and the card such as abundant expectoration cough.

Rhizoma Kaempferiae: latin name kaempferiaerhizoma, for the rhizome of Zingiberaceae Kaempferia plant Rhizoma Kaempferiae, warm in nature, acrid in the mouth, return stomach warp, there is warming middle-JIAO, help digestion, effect of pain relieving, cure mainly trusted subordinate's cold type of pain, stop dyspepsia, traumatic injury, toothache, diet does not disappear to wait and demonstrate,proves.

Caulis Lonicerae: sweet in the mouth, cold in nature, attach to the lung and stomach meridians, there is heat-clearing and toxic substances removing, effect of dispelling wind dredging collateral, cure mainly epidemic febrile disease heating, toxic-heat and blood stasis, carbuncle skin infection, beriberoid pyretic arthralgia, redness and swelling of joints burning pain etc. is demonstrate,proved.

Achillea millefolium: acrid in the mouth; Bitter; Property flat temperature, there is wind-expelling pain-stopping; Effect of promoting blood circulation and detoxication, cures mainly cold, fever; Wind syndrome of head pain; Toothache; Rheumatic arthralgia; Blood stasis amenorrhea; Abdominal part mass in the abdomen; Traumatic injury; Venom; Carbuncle sore tumefacting virus etc. are demonstrate,proved.

The Radix Stemonae: sweet in the mouth, hardship, slightly warm in nature, enters lung meridian, there is nourishing the lung to arrest cough, the effect of delousing parasite killing, Radix Stemonae temperature embellishes but not dry, and property is kept and do not walked, there is again the merit of using pungent drugs for dispersion and bitter drugs for purgation sending down the abnormal ascending QI, being mainly used in newly all for a long time coughing, be especially suitable to consumptive fever chronic cough person, the modern times are used for preventing and treating the cough of chronic bronchitis, the dry cough of pulmonary tuberculosis, pertussis etc. is demonstrate,proved.

Rabdosia rubescens: bitter in the mouth, sweet, cold nature, return lung, stomach, Liver Channel, there is heat-clearing and toxic substances removing, effect of promoting blood circulation and stopping pain, cure mainly the cards such as laryngopharynx swelling and pain, tonsillitis, headache due to common cold, tracheitis, chronic hepatitis, articular rheumatic pain, snake bite and insect sting.

Present invention also offers a kind of feedstuff producing performance for improving shrimp, its raw material includes: fish flour, bean cake, Semen arachidis hypogaeae dregs, meat meal tankage, flour, beer yeast, fish oil, dalcium biphosphate, soybean phospholipid, choline chloride, vitamin C, potassium chloride and feed additive.

Further, described feedstuff is only made up of above-mentioned raw materials.

The weight of each raw material respectively fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg, feed additive 0.05kg in described feedstuff.

Hereinafter adopting embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the process that realizes reaching technique effect can fully understand and implement according to this.

Embodiment 1 Chinese herbal feed additive 1

Rhizoma Coptidis 55g, Semen Euryales 30g, Caulis Spatholobi 30g, Herba Hyperici Sampsonii 25g, Flos Hibisci Rosae-Sinensis 20g, Radix Campanumoeae 30g, Caulis Lonicerae 30g, achillea millefolium 25g, Rabdosia rubescens 25g are respectively crushed into powder; stir mixing by weight; cross 20 mesh sieves; obtain mixture; adopt water boiling and extraction 2 times; mixture for every 1kg; water uses 7L; extract 1.5 hours, sucking filtration every time, remove filtering residue; merging filtrate; the extracting solution obtained adopts Reduced Pressure Concentration Device at 80 DEG C, and vacuum is 0.09Mpa, and solution to be concentrated feed liquor flow velocity is 200ml min^-1When, concentrates, the concentrated extract obtained carries out vacuum drying at 55 DEG C, pulverize, obtain the first powder, by Herba Houttuyniae 25g, Herba Ephedrae 35g, Gaultheria 20g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 15g, Herba Stellariae Alsines 20g, Fructus Pistaciae Verae 25g, Fructus Mume 25g, Herba Apii graveolentis 25g, Herba Eleusines Indicae 25g, Pericarpium Citri Reticulatae 25g, Rhizoma Kaempferiae 25g, Radix Stemonae 15g is respectively crushed into powder, stir mixing by weight, cross 20 mesh sieves, obtain mixture, add the ethanol that determining alcohol is 80% relative to its quality 7 times, it is heated to reflux 3 hours, filter, filtering residue adds the ethanol that determining alcohol is 80% relative to mixture quality 3 times again, it is heated to reflux 3 hours, filter, filtrate is merged, heating concentration removes solvent, obtain and get dry extract, pulverize, obtain the second powder, first powder and the second powder are mixed, obtain Chinese herbal feed additive 1.

Embodiment 2 Chinese herbal feed additive 2

Rhizoma Coptidis 53g, Semen Euryales 28g, Caulis Spatholobi 28g, Herba Hyperici Sampsonii 23g, Flos Hibisci Rosae-Sinensis 18g, Radix Campanumoeae 28g, Caulis Lonicerae 28g, achillea millefolium 23g, Rabdosia rubescens 23g are respectively crushed into powder; stir mixing by weight; cross 20 mesh sieves; obtain mixture; adopt water boiling and extraction 2 times; mixture for every 1kg; water uses 7L; extract 1.5 hours, sucking filtration every time, remove filtering residue; merging filtrate; the extracting solution obtained adopts Reduced Pressure Concentration Device at 80 DEG C, and vacuum is 0.09Mpa, and solution to be concentrated feed liquor flow velocity is 200ml min^-1When, concentrates, the concentrated extract obtained carries out vacuum drying at 55 DEG C, pulverize, obtain the first powder, by Herba Houttuyniae 23g, Herba Ephedrae 33g, Gaultheria 18g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 13g, Herba Stellariae Alsines 18g, Fructus Pistaciae Verae 23g, Fructus Mume 23g, Herba Apii graveolentis 23g, Herba Eleusines Indicae 23g, Pericarpium Citri Reticulatae 23g, Rhizoma Kaempferiae 23g, Radix Stemonae 13g is respectively crushed into powder, stir mixing by weight, cross 20 mesh sieves, obtain mixture, add the ethanol that determining alcohol is 80% relative to its quality 7 times, it is heated to reflux 3 hours, filter, filtering residue adds the ethanol that determining alcohol is 80% relative to mixture quality 3 times again, it is heated to reflux 3 hours, filter, filtrate is merged, heating concentration removes solvent, obtain and get dry extract, pulverize, obtain the second powder, first powder and the second powder are mixed, obtain Chinese herbal feed additive 2.

Embodiment 3 Chinese herbal feed additive 3

Rhizoma Coptidis 57g, Semen Euryales 32g, Caulis Spatholobi 32g, Herba Hyperici Sampsonii 27g, Flos Hibisci Rosae-Sinensis 22g, Radix Campanumoeae 32g, Caulis Lonicerae 32g, achillea millefolium 27g, Rabdosia rubescens 27g are respectively crushed into powder; stir mixing by weight; cross 20 mesh sieves; obtain mixture; adopt water boiling and extraction 2 times; mixture for every 1kg; water uses 7L; extract 1.5 hours, sucking filtration every time, remove filtering residue; merging filtrate; the extracting solution obtained adopts Reduced Pressure Concentration Device at 80 DEG C, and vacuum is 0.09Mpa, and solution to be concentrated feed liquor flow velocity is 200ml min^-1When, concentrates, the concentrated extract obtained carries out vacuum drying at 55 DEG C, pulverize, obtain the first powder, by Herba Houttuyniae 27g, Herba Ephedrae 37g, Gaultheria 22g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 17g, Herba Stellariae Alsines 22g, Fructus Pistaciae Verae 27g, Fructus Mume 27g, Herba Apii graveolentis 27g, Herba Eleusines Indicae 27g, Pericarpium Citri Reticulatae 27g, Rhizoma Kaempferiae 27g, Radix Stemonae 17g is respectively crushed into powder, stir mixing by weight, cross 20 mesh sieves, obtain mixture, add the ethanol that determining alcohol is 80% relative to its quality 7 times, it is heated to reflux 3 hours, filter, filtering residue adds the ethanol that determining alcohol is 80% relative to mixture quality 3 times again, it is heated to reflux 3 hours, filter, filtrate is merged, heating concentration removes solvent, obtain and get dry extract, pulverize, obtain the second powder, first powder and the second powder are mixed, obtain Chinese herbal feed additive 3.

The toxicity test analysis of embodiment 4 Chinese herbal feed additive

Acute toxicity

Experimental animal: white mice 60, Kunming kind, male and female half and half, body weight is 18��22g.

Packet and administration: be randomly divided into 4 groups, often group 15, is added in distilled water by Chinese herbal feed additive prepared by embodiment 1 subsequently, it is configured to 0.05g/ml solution, gavage is 3 groups of mices wherein, the 4th group of mouse stomach distilled water, fasting 6h before mice lavage administration. It is administered 3 times in 24h, every minor tick 6h, group single dose respectively 2000mg/kg, 4000mg/kg and the 6000mg/kg of 3 groups of gavage Chinese herbal feed additives, after administration, observes its intoxication conditions and animal dead number by the normal 7d that raises also continuously. LD is calculated by simplifying karber's method₅₀And 95% fiducial limit.

Result: white mice is administered after 3 times in the observation period of continuous 7d in 24h, and obvious poisoning symptom all do not occur in all white mice, the phenomena of mortality do not occur yet. When being administered accumulated dose and reaching 18000.0mg/kg, white mice does not occur death yet, it was shown that Chinese medicine powder chicken coccidia powder is to white mice per os LD₅₀> 18000.0mg/kg.

Conclusion: according to toxicology test principle, as the LD of the general acute toxicity test of tested material₅₀During > 10000.0mg/kg, it may not be necessary to carry out other toxicity tests again. By five grade standards of World Health Organization (WHO) acute toxicity grading criteria relevant to China, Chinese herbal feed additive is to white mice per os LD₅₀> 18000.0mg/kg, it was shown that it belongs to micro-poison level (actual nontoxic level) material.

Long term toxicity test

This test application SD rat 80, if matched group and 14.5g, 29g, 58g crude drug/kg dosage group, continuous gastric infusion 4 weeks, the impact on animal indices of the Chinese herbal feed additive of the observation present invention. Result of the test shows, successive administration 4 weeks, and the body weight of rat, food ration, peripheral hemogram, clotting time, electrocardiogram, organ index etc. are all had no significant effect by the Chinese herbal feed additive of the present invention; Blood biochemical ten binomial index determining result, is administered 4 weeks low dose group BiLi values higher than matched group (P < 0.05), it is possible to relevant with wherein three serum sample haemolysis; Low dose group, middle dosage group TP value are higher than matched group (P < 0.05), but all within normal physiologic range, other index compares no significant difference with matched group. Convalescent period each administration group indices measured value compares equal no significant difference with matched group. The each internal organs gross examination of skeletal muscle no abnormality seen of each group.

Embodiment 5 feed additive

The Chinese herbal feed additive 1 1kg embodiment 1 prepared, zinc methionine 50g, ferrous methionine 50g and glutamine 75g send in mixer and are uniformly mixed, it is thus achieved that compound, the compound rotary drum dryer of temperature 200 DEG C is dried 3 minutes, the water content making compound is 5%, prepares semi-finished product, is sieved by semi-finished product, the aperture that first time sieves is 1mm, obtain finished product, filter out the little granule more than 1mm and again pulverize, again sieve, mix with finished product above, it is thus achieved that feed additive.

Embodiment 6 feedstuff 1

Chinese herbal feed additive 0.05kg prepared by fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg, embodiment 1 is pulverized respectively, cross mix homogeneously after 60 mesh sieves, make 1.0mm diameter particle feedstuff, in 90 DEG C of baking ovens, first toast 2h, then drain in air-conditioned room, obtain feedstuff 1 ,-20 DEG C of refrigerators seal and saves backup.

Embodiment 7 feedstuff 2

Feed additive 0.05kg prepared by fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg, embodiment 5 is pulverized respectively, cross mix homogeneously after 60 mesh sieves, make 1.0mm diameter particle feedstuff, in 90 DEG C of baking ovens, first toast 2h, then drain in air-conditioned room, obtain feedstuff 2 ,-20 DEG C of refrigerators seal and saves backup.

Comparative example 1 feedstuff 3

Fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg are pulverized respectively, cross mix homogeneously after 60 mesh sieves, make 1.0mm diameter particle feedstuff, in 90 DEG C of baking ovens, first toast 2h, then drain in air-conditioned room, obtain feedstuff 3 ,-20 DEG C of refrigerators seal and saves backup.

Embodiment 7 raises situation

Laboratory animal and feeding and management

After prawn raises and train 7d, randomly selecting specification consistent, physically fit, the prawn of weight (8.0 �� 0.2) g is tested. Experiment is divided into 3 process groups, often process group 4 repetition, is often repeated as a bite net cage (2.0m �� 1.0m �� 1.2m), totally 12 mouthfuls of net cages, is respectively placed in 2 mouthfuls of water mud sumps (5m �� 3m �� 1.5m), and each net cage puts shrimp 80 tail. During cultivation, day feeding volume is the 4%��5% of shrimp body weight, and be adjusted accordingly according to weather and the situation of searching for food, make the throwing amount of raising that the maintenance of each net cage is basically identical, it is 3 times/day of (6:00 that frequency is raised in throwing, 12:00 and 18:00), inflate round the clock, change water 1/3 every 3d, during experiment, water temperature is 24��29 DEG C, dissolved oxygen > 4.5mg/L, ammonia nitrogen��0.2mg/L, nitrite��0.1mg/L, culture experiment totally 42 days, matched group uses the feedstuff 3 of comparative example 1 preparation, tests 1 group and uses embodiment 6 to prepare feedstuff 1, tests 2 groups of feedstuffs 2 using embodiment 7 preparation.

Testing index and method

After culture experiment terminates, shrimp body hunger 24h, weighs by net cage and counts, calculating following index.

Rate of body weight gain (WG, %)=(opisthosoma quality-first weight) �� 100/ is weight just

Survival rate (SR, %)=off-test Shi Xia mantissa �� 100/ test

Start Shi Fang shrimp mantissa

Feed coefficient (FCR)=food ration/(opisthosoma quality-first weight+dead shrimp weight)

Serum non-specific immunity index

Every net cage takes 5 tail shrimps, inserts heart extracting blood lymph sample (10% Disodium oxalate. makees anticoagulant) after prawn head cuirass, and a sample merged into by the hemolymph sample of 3 tail shrimps, and 5000r/min is centrifuged 10min, takes serum standby in 80 DEG C of freezings, measures following index.

The colorimetric method for determining that phenol oxidase (PO) is substrate with L-3,4 dihydroxyphenylalanine, lysozyme (LSZ) adopts turbidimetry, superoxide dismutase (SOD) adopts hydroxylamine assay, malonaldehyde (MDA) adopts thiobarbituricacid��-(TBA) method, and test kit is all purchased from Nanjing and builds up biological study institute. Alkali phosphatase (ALP) adopts AMP buffer method, and test kit is purchased from Mairui Biological Medical Electronic Co., Ltd., Shenzhen, and instrument adopts and steps auspicious BS array of biochemical analyser (stepping auspicious BS-200).

Muscular components and cathepsin

Every net cage takes 6 tail shrimps, gathers full intestinal, hepatopancrease, harmonization of the stomach muscle, and the corresponding tissue of 3 tail shrimps merges into a sample, and 80 DEG C of freezings are standby, are determined as follows index.

Muscular components

Moisture adopts 105 DEG C of oven drying methods (GB6435-86), and crude protein adopts Kjeldahl method (GB/T6432-94), and crude fat adopts chloroform-methanol method, and ash adopts 550 DEG C of calcination methods (GB/T6438-92).

Collagen of muscle

Adopting Nanjing to build up biological study institute test kit (acid hydroxyproline method), hydroxyproline produced oxide under the effect of oxidant presents aubergine with dimethylaminobenzaldehyde effect, the depth calculation content according to its colour generation.

Cathepsin

Adopt Forint phenol method, proteases for decomposing casein, generate the aminoacid containing phenolic group and react with Folin-Phenol reagent au bleu, enzyme activity can be predicted from the blue depth. At 40 DEG C, caseinhydrolysate per minute produces 1 microgram tyrosine, is defined as 1 protease activity unit of force.

Data statistics

Experimental data with mean+SD (�� SD) represent, adopting SPSS17.0 software that data carry out single factor test variance statistic analysis, group difference adopts Duncan multiple comparisons, and significant level is P < 0.05.

Result

Growth performance compares, and result is in Table 1.

Growth performance is affected by table 1 Chinese herbal feed additive

As it can be seen from table 1 adopt Chinese herbal feed additive provided by the invention can improve the weight (P < 0.05) of prawn, feed coefficient reduces (P < 0.05).

Serum non-specific immunity index, result is in Table 2.

The impact on serum non-specific immunity index of table 2 Chinese herbal feed additive

From table 2 it can be seen that adopt Chinese herbal feed additive provided by the invention can significantly improve SOD, LSZ, ALP, PO activity (P < 0.05) of prawn, the function of enhancing human body immunity.

Proteinase activity, result is in Table 3.

The impact on protease of table 3 Chinese herbal feed additive

From table 3 it can be seen that adopt Chinese herbal feed additive provided by the invention can improve the activity (P < 0.05) of erepsin, pepsin regulating liver-QI pancreatic protease.

Muscular components affects, and result is in Table 4.

Muscular components is affected by table 4 Chinese herbal feed additive

From table 4, it can be seen that adopt Chinese herbal feed additive provided by the invention can improve the content of collagen protein and crude protein, reduce the content of moisture, ash and crude fat.

All above-mentioned primary these intellectual properties of enforcement, do not set this new product of enforcement and/or the new method that limit other forms. Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status. But, all modifications or transformation belong to the right of reservation based on new product of the present invention.

The above, be only presently preferred embodiments of the present invention, is not the restriction that the present invention makees other form, and any those skilled in the art are changed or be modified as the Equivalent embodiments of equivalent variations possibly also with the technology contents of the disclosure above. But every without departing from technical solution of the present invention content, according to any simple modification, equivalent variations and remodeling that above example is made by the technical spirit of the present invention, still fall within the protection domain of technical solution of the present invention.

Claims

1. the feed additive producing performance for improving shrimp, it is characterised in that: raw material includes for improving the shrimp production Chinese herbal feed additive of performance, zinc methionine, ferrous methionine and glutamine.

2. feed additive as claimed in claim 1, it is characterised in that: the weight of each raw material respectively Chinese herbal feed additive 1kg, zinc methionine 50g, ferrous methionine 50g and glutamine 75g in described feed additive.

3. feed additive as claimed in claim 1 or 2, it is characterised in that: the crude drug of described Chinese herbal feed additive includes Rhizoma Coptidis, Semen Euryales, Herba Houttuyniae, Caulis Spatholobi, Herba Ephedrae, Gaultheria, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati), Herba Hyperici Sampsonii, Herba Stellariae Alsines, Fructus Pistaciae Verae, Fructus Mume, Flos Hibisci Rosae-Sinensis, Herba Apii graveolentis, Herba Eleusines Indicae, Radix Campanumoeae, Pericarpium Citri Reticulatae, Rhizoma Kaempferiae, Caulis Lonicerae, achillea millefolium, the Radix Stemonae, Rabdosia rubescens.

4. the feed additive as described in claims 1 to 3, it is characterized in that: the weight of each crude drug respectively Rhizoma Coptidis 50g ~ 60g in described Chinese herbal feed additive, Semen Euryales 25g ~ 35g, Herba Houttuyniae 20g ~ 30g, Caulis Spatholobi 25g ~ 35g, Herba Ephedrae 30g ~ 40g, Gaultheria 15g ~ 25g, Flos Ixorae Chinensis (Cacumen et Folium Clerodendri paniculati) 10g ~ 20g, Herba Hyperici Sampsonii 20g ~ 30g, Herba Stellariae Alsines 15g ~ 25g, Fructus Pistaciae Verae 20g ~ 30g, Fructus Mume 20g ~ 30g, Flos Hibisci Rosae-Sinensis 15g ~ 25g, Herba Apii graveolentis 20g ~ 30g, Herba Eleusines Indicae 20g ~ 30g, Radix Campanumoeae 25g ~ 35g, Pericarpium Citri Reticulatae 20g ~ 30g, Rhizoma Kaempferiae 20g ~ 30g, Caulis Lonicerae 25g ~ 35g, achillea millefolium 20g ~ 30g, Radix Stemonae 10g ~ 20g and Rabdosia rubescens 20g ~ 30g.

5. the preparation method of feed additive described in Claims 1-4, it is characterised in that including:

The first step, prepares described Chinese herbal feed additive;

6. the preparation method of feed additive described in claim 1 to 5, it is characterised in that:

The described first step further specifically,

7. the feedstuff producing performance for improving shrimp, it is characterised in that: raw material includes fish flour, bean cake, Semen arachidis hypogaeae dregs, meat meal tankage, flour, beer yeast, fish oil, dalcium biphosphate, soybean phospholipid, choline chloride, vitamin C, potassium chloride and feed additive.

8. the feedstuff described in claim 7, it is characterised in that: the weight of each raw material respectively fish flour 2.3kg, bean cake 2.0kg, Semen arachidis hypogaeae dregs 1.0kg, meat meal tankage 0.5kg, flour 2.7kg, beer yeast 0.3kg, fish oil 0.2kg, dalcium biphosphate 0.2kg, soybean phospholipid 0.15kg, choline chloride 0.06kg, vitamin C 0.015kg, potassium chloride 0.05kg, feed additive 0.05kg in described feedstuff.

9. the preparation method of feedstuff described in claim 7 or 8, it is characterised in that:

Described raw material pulverizing is crossed mix homogeneously after 60 mesh sieves, makes 1.0mm diameter particle feedstuff, in 90 DEG C of baking ovens, first toast 2h, then drain in air-conditioned room ,-20 DEG C of refrigerators seal and saves backup.