CN105601398A - Novel raw material culture medium of gastrodia elata and armillaria mella and preparation method of novel raw material culture medium - Google Patents
Novel raw material culture medium of gastrodia elata and armillaria mella and preparation method of novel raw material culture medium Download PDFInfo
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- CN105601398A CN105601398A CN201610146460.4A CN201610146460A CN105601398A CN 105601398 A CN105601398 A CN 105601398A CN 201610146460 A CN201610146460 A CN 201610146460A CN 105601398 A CN105601398 A CN 105601398A
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Abstract
The invention discloses a novel raw material culture medium of gastrodia elata and armillaria mella and a preparation method of the novel raw material culture medium. The novel raw material culture medium is prepared from the following raw materials in parts by weight: 200 to 220 parts of straws, 2 to 6 parts of gypsum powder, 4 to 8 parts of soybean powder, 2 to 6 parts of wheat bran, 2 to 6 parts of sawdust and 8 to 12 parts of nutritional agents. The preparation method comprises the steps: crushing, mixing, carbonizing and immersing. Since the novel raw material culture medium is sufficient in raw material nutrients, after the special carbonization treatment, the effective nutrient supply time can be increased to more than one year; and the culture medium is used for supplying the nutrients so as to plant the gastrodia elata, so that the yield of the gastrodia elata can be effectively increased. The cultivated gastrodiae elata has the characteristics of thick shape, large size, good growth and stable quality; and the effective biological conversion rate can reach 30 to 40 percent.
Description
Technical field
The present invention relates to technique for cultivation of Gastrodia elata field, be specifically related to a kind of Tianma Mihuanjun novel formerMaterial culture medium and preparation method thereof.
Background technology
In the cultivating and growing process of rhizoma Gastrodiae, it includes generative propagation and two kinds of plantations of vegetative propagationMethod, in sexual reproduction process, in order to reduce the cultivation cost of rhizoma Gastrodiae, increases the product of rhizoma GastrodiaeAmount, except the halimasch material of high-quality need to be provided, making halimasch material is that gastrodin cultivation was plantedIn journey, providing outside sufficient nutrition, is also heavy to closing for germination rate, the survival rate of rhizoma Gastrodiae plantationWant.
In order to improve the output of rhizoma Gastrodiae, just must improve the bacterium rate that connects, how to improve that to connect bacterium rate be to carryHigh Output of Gastrodia elata key factor, the inventor, by a large amount of research and experiment, has proposed oneNovel raw material culture medium is cultivated close ring bacterium, for rhizoma Gastrodiae generative propagation protocorm has increasedMore Inoculation machine rate, thus reach the object that improves Output of Gastrodia elata, and can also solve traditionRhizoma Gastrodiae is cultivated the problem to vegetation ecological damages, gastrodin cultivation cost is high.
Summary of the invention
For solving the problems of the technologies described above, the invention provides the novel raw material of a kind of Tianma MihuanjunCulture medium and preparation method thereof.
The present invention is achieved by the following technical programs:
The novel raw material culture medium of a kind of Tianma Mihuanjun, it is made up of following weight portion raw material:Stalk 200-220 part, land plaster 2-6 part, soy meal 4-8 part, wheat bran 2-6 part, sawdust 2-6 part,Nutritional agents 8-12 part.
The novel raw material culture medium of described Tianma Mihuanjun is made up of following weight portion raw material: stalk205-215 part, land plaster 3-5 part, soy meal 5-7 part, wheat bran 3-5 part, sawdust 3-5 part, battalionSupport agent 9-11 part.
The novel raw material culture medium of described Tianma Mihuanjun is made up of following weight portion raw material: stalk210 parts, 4 parts of land plasters, 6 parts of soy meals, 4 parts, wheat bran, 4 parts, sawdust, 10 parts of nutritional agents.
Described stalk is at least one in maize straw, wheat stalk, rice straw.
That to be ardealite obtain through high-temperature calcination processing and ball-milling treatment described land plaster is anhydrousLand plaster.
Described high-temperature calcination processing is the temperature lower calcination at 200-250 DEG C by ardealiteAfter 60-80min.
Described ball-milling treatment is to be milled to putting into ball mill through the gypsum of calcination processing100-300 order obtains anhydrous plaster powder.
Described nutritional agents is to be prepared from by following parts by weight of raw materials raw material: 4 parts of cotton seed hulls, woodConsider 2 parts, 2 parts of corn flour, 1 part, green grass powder to be worth doing, after when preparation, cotton seed hulls and wood chip being pulverized with beautifulGround rice, green grass powder mix.
The present invention also provides the preparation method of the novel raw material culture medium of above-mentioned Tianma Mihuanjun,The method comprises the following steps:
A, be pulverized and mixed: by stalk, wheat bran, sawdust powder be broken to after 100-300 order with land plaster,Soy meal, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 80-90 DEG C carbonization machine burn to tableWhen face surface is coke yellow;
C, immersion treatment: will put into the Fructus Hordei Germinatus syrup of 2-4% through the batch mixing piece of carbonization treatmentBe dipped to water suction and pull the cultivation of carrying out halimasch piece out after saturated, when cultivation by female bacterium and culture blockEducating bacterium method according to tradition cultivates.
Beneficial effect of the present invention is: in novel raw material culture medium prepared by the present invention due toMaterial nutrient component abundance, after special carbonization processing is processed, when it is effectively supportedBetween can bring up to more than 1 year, adopting basal culture medium to support to carry out the plantation of rhizoma Gastrodiae can be effectiveThe output of its rhizoma Gastrodiae of raising, and the rhizoma Gastrodiae cultivating has that thick shape is large, well-grown, matterMeasure stable feature, its effective biological conversion ratio can reach 30-40%, basal culture medium preparationMethod is simple, cost is low, also overcome traditional rhizoma Gastrodiae cultivates asking that vegetation ecological is damagedTopic, and by ardealite through processing process after for serving as the raw material of basal culture medium, effectively subtractLacked the stacking problem of ardealite, reduced the cost of culture medium, basal culture medium has goodAdsorption capacity, can absorb nutrient long-term preservation, for close ring bacteria growing provides guarantee, separatesTraditional rhizoma Gastrodiae of having determined is cultivated the problem that bacterium rate is low, yield poorly that connects.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is further described, but requirementDescribed in the scope of protection is not limited to.
Embodiment mono-
Formula: 200 parts, 2 parts of land plasters, 4 parts of soy meals, 2 parts, wheat bran, 2 parts, sawdust, battalionSupport 8 parts of agent.
Land plaster preparation: ardealite, after the temperature lower calcination 60min of 200 DEG C, is put into ball millingIn machine, be milled to 100 orders and obtain anhydrous plaster powder.
The novel raw material culture medium preparation of Tianma Mihuanjun:
A, be pulverized and mixed: stalk, wheat bran, sawdust powder are broken to after 100 orders and land plaster, largeBean powder, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 80 DEG C carbonization machine burn to surfaceWhen surface is coke yellow;
C, immersion treatment: the Fructus Hordei Germinatus syrup of putting into 2% through the batch mixing piece of carbonization treatment is soakedBubble pulls to absorbing water after saturated the cultivation of carrying out halimasch piece out, when cultivation, female bacterium and culture block is pressedEducating bacterium method according to tradition cultivates.
Embodiment bis-
Formula: 220 parts, 6 parts of land plasters, 8 parts of soy meals, 6 parts, wheat bran, 6 parts, sawdust, battalionSupport 12 parts of agent.
Land plaster preparation: ardealite, after the temperature lower calcination 80min of 250 DEG C, is put into ball millingIn machine, be milled to 300 orders and obtain anhydrous plaster powder.
The novel raw material culture medium preparation of Tianma Mihuanjun:
A, be pulverized and mixed: stalk, wheat bran, sawdust powder are broken to after 300 orders and land plaster, largeBean powder, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 90 DEG C carbonization machine burn to surfaceWhen surface is coke yellow;
C, immersion treatment: the Fructus Hordei Germinatus syrup of putting into 4% through the batch mixing piece of carbonization treatment is soakedBubble pulls to absorbing water after saturated the cultivation of carrying out halimasch piece out, when cultivation, female bacterium and culture block is pressedEducating bacterium method according to tradition cultivates.
Embodiment tri-
Formula: 205 parts of stalks, 3 parts of land plasters, 5 parts of soy meals, 3 parts, wheat bran, 3 parts, sawdust,9 parts of nutritional agents.
Land plaster preparation: ardealite, after the temperature lower calcination 60min of 200 DEG C, is put into ball millingIn machine, be milled to 100 orders and obtain anhydrous plaster powder.
The novel raw material culture medium preparation of Tianma Mihuanjun:
A, be pulverized and mixed: stalk, wheat bran, sawdust powder are broken to after 100 orders and land plaster, largeBean powder, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 80 DEG C carbonization machine burn to surfaceWhen surface is coke yellow;
C, immersion treatment: the Fructus Hordei Germinatus syrup of putting into 2% through the batch mixing piece of carbonization treatment is soakedBubble pulls to absorbing water after saturated the cultivation of carrying out halimasch piece out, when cultivation, female bacterium and culture block is pressedEducating bacterium method according to tradition cultivates.
Embodiment tetra-
Formula: 215 parts of stalks, 5 parts of land plasters, 7 parts of soy meals, 5 parts, wheat bran, 5 parts, sawdust,11 parts of nutritional agents.
Land plaster preparation: ardealite, after the temperature lower calcination 80min of 250 DEG C, is put into ball millingIn machine, be milled to 300 orders and obtain anhydrous plaster powder.
The novel raw material culture medium preparation of Tianma Mihuanjun:
A, be pulverized and mixed: stalk, wheat bran, sawdust powder are broken to after 300 orders and land plaster, largeBean powder, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 80-90 DEG C carbonization machine burn to tableWhen face surface is coke yellow;
C, immersion treatment: the Fructus Hordei Germinatus syrup of putting into 4% through the batch mixing piece of carbonization treatment is soakedBubble pulls to absorbing water after saturated the cultivation of carrying out halimasch piece out, when cultivation, female bacterium and culture block is pressedEducating bacterium method according to tradition cultivates.
Embodiment five
Formula: 210 parts of stalks, 4 parts of land plasters, 6 parts of soy meals, 4 parts, wheat bran, 4 parts, sawdust,10 parts of nutritional agents.
Land plaster preparation: ardealite, after the temperature lower calcination 70min of 225 DEG C, is put into ball millingIn machine, be milled to 200 orders and obtain anhydrous plaster powder.
The novel raw material culture medium preparation of Tianma Mihuanjun:
A, be pulverized and mixed: stalk, wheat bran, sawdust powder are broken to after 200 orders and land plaster, largeBean powder, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 85 DEG C carbonization machine burn to surfaceWhen surface is coke yellow;
C, immersion treatment: the Fructus Hordei Germinatus syrup of putting into 3% through the batch mixing piece of carbonization treatment is soakedBubble pulls to absorbing water after saturated the cultivation of carrying out halimasch piece out, when cultivation, female bacterium and culture block is pressedEducating bacterium method according to tradition cultivates.
Claims (9)
1. the novel raw material culture medium of Tianma Mihuanjun, is characterized in that: it by belowWeight portion raw material composition: stalk 200-220 part, land plaster 2-6 part, soy meal 4-8 part, wheat bran2-6 part, sawdust 2-6 part, nutritional agents 8-12 part.
2. the novel raw material culture medium of Tianma Mihuanjun according to claim 1, its featureBe: the novel raw material culture medium of described Tianma Mihuanjun is made up of following weight portion raw material: strawStalk 205-215 part, land plaster 3-5 part, soy meal 5-7 part, wheat bran 3-5 part, sawdust 3-5 part,Nutritional agents 9-11 part.
3. the novel raw material culture medium of Tianma Mihuanjun according to claim 1, its featureBe: the novel raw material culture medium of described Tianma Mihuanjun is made up of following weight portion raw material: straw210 parts of stalks, 4 parts of land plasters, 6 parts of soy meals, 4 parts, wheat bran, 4 parts, sawdust, 10 parts of nutritional agents.
4. according to the arbitrary described novel raw material culture medium of Tianma Mihuanjun of claim 1-3,It is characterized in that: described stalk is at least one in maize straw, wheat stalk, rice strawKind.
5. according to the arbitrary described novel raw material culture medium of Tianma Mihuanjun of claim 1-3,It is characterized in that: described land plaster is that ardealite obtains through high-temperature calcination processing and ball-milling treatmentAnhydrous plaster powder.
6. the novel raw material culture medium of Tianma Mihuanjun according to claim 5, its featureBe: described high-temperature calcination processing is the temperature lower calcination at 200-250 DEG C by ardealiteAfter 60-80min.
7. the novel raw material culture medium of Tianma Mihuanjun according to claim 5, its featureBe: described ball-milling treatment is to be milled to putting into ball mill through the gypsum of calcination processing100-300 order obtains anhydrous plaster powder.
8. according to the arbitrary described novel raw material culture medium of Tianma Mihuanjun of claim 1-3,It is characterized in that: described nutritional agents is to be prepared from by following parts by weight of raw materials raw material: cotton seed hulls4 parts, 2 parts of wood chips, 2 parts of corn flour, 1 part, green grass powder, when preparation pulverize cotton seed hulls and wood chipMix with corn flour, green grass powder afterwards.
9. the novel raw material of the Tianma Mihuanjun as described in as arbitrary in claim 1-3 are cultivatedThe preparation method of base, is characterized in that: the method comprises the following steps:
A, be pulverized and mixed: by stalk, wheat bran, sawdust powder be broken to after 100-300 order with land plaster,Soy meal, nutritional agents fully mix and obtain batch mixing;
B, carbonization treatment: batch mixing is put into forming press compacting and grow into 40cm, wide is 10cm,Height is the batch mixing piece of 10cm, by batch mixing piece put into temperature be 80-90 DEG C carbonization machine burn to tableWhen face surface is coke yellow;
C, immersion treatment: will put into the Fructus Hordei Germinatus syrup of 2-4% through the batch mixing piece of carbonization treatmentBe dipped to water suction and pull the cultivation of carrying out halimasch piece out after saturated, when cultivation by female bacterium and culture blockEducating bacterium method according to tradition cultivates.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416752A (en) * | 2016-09-30 | 2017-02-22 | 朱良君 | Artificial cultivation method for arimillaria mellea |
| CN106495810A (en) * | 2016-11-11 | 2017-03-15 | 贵州黔安源药科技发展有限公司 | A kind of Armillaria mellea culture base material for gastrodin cultivation and preparation method thereof |
| CN106538225A (en) * | 2016-11-30 | 2017-03-29 | 贵州黔聚隆农业科技开发有限公司 | A kind of rhizoma Gastrodiae mountain planting method |
| CN108277168A (en) * | 2018-03-30 | 2018-07-13 | 普安县红星村康泰中草药种植农民专业合作社 | A kind of Rhizoma Gastrodiae plantation halimasch culture medium and preparation method thereof |
| CN108812193A (en) * | 2018-06-04 | 2018-11-16 | 安徽亚泰药业有限公司 | A kind of gastrodin cultivation matrix and preparation method thereof |
| CN113099948A (en) * | 2021-03-29 | 2021-07-13 | 中国中医科学院中药研究所 | Soybean straw culture medium for culturing armillaria mellea and armillaria mellea production method |
| CN114287286A (en) * | 2022-02-22 | 2022-04-08 | 康县宁康生物科技有限公司 | Cultivation method of gastrodia elata armillaria mellea |
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| CN102498845A (en) * | 2011-10-14 | 2012-06-20 | 张本强 | Indoor three-dimensional tank type gastrodia tuber culture method |
| CN103798056A (en) * | 2013-06-09 | 2014-05-21 | 程立君 | Production method of gastrodia elata seed germinating strains |
| CN104541970A (en) * | 2014-12-18 | 2015-04-29 | 李志芳 | Outdoor tall gastrodia tuber cultivating method in northern area |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498845A (en) * | 2011-10-14 | 2012-06-20 | 张本强 | Indoor three-dimensional tank type gastrodia tuber culture method |
| CN103798056A (en) * | 2013-06-09 | 2014-05-21 | 程立君 | Production method of gastrodia elata seed germinating strains |
| CN104541970A (en) * | 2014-12-18 | 2015-04-29 | 李志芳 | Outdoor tall gastrodia tuber cultivating method in northern area |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416752A (en) * | 2016-09-30 | 2017-02-22 | 朱良君 | Artificial cultivation method for arimillaria mellea |
| CN106495810A (en) * | 2016-11-11 | 2017-03-15 | 贵州黔安源药科技发展有限公司 | A kind of Armillaria mellea culture base material for gastrodin cultivation and preparation method thereof |
| CN106538225A (en) * | 2016-11-30 | 2017-03-29 | 贵州黔聚隆农业科技开发有限公司 | A kind of rhizoma Gastrodiae mountain planting method |
| CN108277168A (en) * | 2018-03-30 | 2018-07-13 | 普安县红星村康泰中草药种植农民专业合作社 | A kind of Rhizoma Gastrodiae plantation halimasch culture medium and preparation method thereof |
| CN108812193A (en) * | 2018-06-04 | 2018-11-16 | 安徽亚泰药业有限公司 | A kind of gastrodin cultivation matrix and preparation method thereof |
| CN113099948A (en) * | 2021-03-29 | 2021-07-13 | 中国中医科学院中药研究所 | Soybean straw culture medium for culturing armillaria mellea and armillaria mellea production method |
| CN114287286A (en) * | 2022-02-22 | 2022-04-08 | 康县宁康生物科技有限公司 | Cultivation method of gastrodia elata armillaria mellea |
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Application publication date: 20160525 |