CN105565966A - Microbial fertilizer rich in nucleic acid and preparation method thereof - Google Patents

Abstract

The invention discloses a microbial fertilizer rich in nucleic acid. The microbial fertilizer is prepared from the following raw materials in parts by weight: 50-52 parts of mushroom bran, 2-3 parts of magnesium sulfate, 6-7 parts of quartz stone powder, 3-4 parts of shell powder, 8-9 parts of nucleic acid waste water, 25-30 parts of attapulgite, 5-5.5 parts of azotobacter chroococcum, 70-72 parts of phosphate buffer solution with pH of 7.0, 5-6 parts of sodium alginate, 4-5 parts of calcium chloride and 2-3 parts of chitosan. The mushroom bran is taken as a microbial carrier, so that more organic matters are provided for strain growth; after the mushroom bran is puffed, the hydroscopicity is enhanced, and a wet living environment is provided for microorganisms; the sodium alginate is adopted for embedding, so that the salt tolerance, acid and alkali tolerance, cold and heat tolerance and drug tolerance of the microorganisms can be enhanced; the chitosan is used for enveloping, so that the defect of low mechanical strength of the sodium alginate is overcome; moreover, the microbial fertilizer is rich in nucleic acid, so that the crop activity can be enhanced, and the yield is increased.

Description

A kind of microbial fertilizer being rich in nucleic acid and preparation method thereof

Technical field

The present invention relates to technical field of fertilizers, particularly relate to a kind of microbial fertilizer being rich in nucleic acid and preparation method thereof.

Background technology

Along with the develop rapidly of world economy, the consumption of the mineral fuel such as oil grows with each passing day, and the energy dilemma extreme shock caused thus fertilizer industry, and a large amount of production of chemical fertilizer and use harm ecotope balance and human health.And microbial fertilizer has high, the reproducible feature of fertilizer efficiency, the ecological agriculture advocated with modern agriculture, Organic farming are coincide.Microbial fertilizer carrier in the market has vermiculite, perlite, peat and fertilizer etc. that some become thoroughly decomposed, wherein using peat as generally.And peat is under special Marsh Wetland environment, plant residue through complicated and slowly biochemical action, to build up and ulmification is formed.It is non-renewable that it has short-term as the natural Mineral resources of one.Bacterium chaff is the residue waste material after Edible Fungi, and the traditional method of process bacterium chaff abandons or burns, and not only causes the wasting of resources, cause mould and pest infestation simultaneously, thus cause environmental pollution.Therefore, how effectively to utilize edible fungus bran, grown fat as treasured, become problem demanding prompt solution.

Summary of the invention

The object of the invention is exactly the defect in order to make up prior art, provides a kind of microbial fertilizer being rich in nucleic acid and preparation method thereof.

The present invention is achieved by the following technical solutions:

Be rich in a microbial fertilizer for nucleic acid, be made up of the raw material of following weight part:

Bacterium chaff 50-52, magnesium sulfate 2-3, quartz powder 6-7, oyster shell whiting 3-4, nucleic acid waste water 8-9, attapulgite 25-30, the phosphate buffered saline buffer 70-72 of azotobacter chroococcum 5-5.5, pH7.0, sodium alginate 5-6, calcium chloride 4-5, chitosan 2-3.

The described preparation method being rich in the microbial fertilizer of nucleic acid, comprises the following steps:

(1) by expanded for bacterium chaff twin-screw extruder rear cooling crush, crossing aperture is the sieve of 0.25mm, continuous sterilization 1 hour at 121 DEG C, dries;

(2) phosphate buffered saline buffer of pH7.0 and step (1) gained material are mixed, access azotobacter chroococcum, cultivates 7 days in the constant temperature biochemical cultivation case at 28 DEG C;

(3) calcium chloride is added sterile distilled water and be configured to the solution that concentration is 4%, obtain calcium chloride solution; Sodium alginate is added sterile distilled water heating for dissolving, be configured to the solution that concentration is 2%, to be cooled to adding step (2) gained material when 40 DEG C, abundant mixing, is then dropwise added dropwise in calcium chloride solution, leaves standstill crosslinked 1-1.5 hour, with 0.3mol/L glutaraldehyde solution process 1-1.5 hour, wash with the sodium chloride solution of 0.9% again, then solidify 24 hours at 4 DEG C, for subsequent use;

(4) chitosan is added water be mixed with the chitosan solution that concentration is 0.3%, then the coating process in chitosan solution of step (3) gained material was taken out after 0.5 hour, mix with leftover materials, add water and make material moisture reach 20%, granulation, to obtain final product.

Advantage of the present invention is: the application adopts bacterium chaff as microbe carrier, higher compared to its organic content of conventional carriers peat, can be growth and more organicly provide, and bacterium chaff is through expanded, structure organization is loosened, improve water-absorbent, moistening living environment can be provided for microorganism attached thereto, and when cultivating, add phosphate buffered saline buffer maintenance neutrality, sodium alginate is adopted to embed, the salt tolerance of microorganism can be improved, resistance to acids and bases, cold-resistant hot and resistance, again with chitosan coated, overcome the weakness that sodium alginate physical strength is little, in addition, nucleic acid is rich in the present invention, crop activity can be strengthened, improve its nutrient delivery function, delay crop to decay, increase yield.

Embodiment

Be rich in a microbial fertilizer for nucleic acid, be made up of the raw material of following weight part (kilogram):

The phosphate buffered saline buffer 70 of bacterium chaff 50, magnesium sulfate 2, quartz powder 6, oyster shell whiting 3, nucleic acid waste water 8, attapulgite 25, azotobacter chroococcum 5, pH7.0, sodium alginate 5, calcium chloride 4, chitosan 2.

The described preparation method being rich in the microbial fertilizer of nucleic acid, comprises the following steps:

(1) by expanded for bacterium chaff twin-screw extruder rear cooling crush, crossing aperture is the sieve of 0.25mm, continuous sterilization 1 hour at 121 DEG C, dries;

(2) phosphate buffered saline buffer of pH7.0 and step (1) gained material are mixed, access azotobacter chroococcum, cultivates 7 days in the constant temperature biochemical cultivation case at 28 DEG C;

(3) calcium chloride is added sterile distilled water and be configured to the solution that concentration is 4%, obtain calcium chloride solution; Sodium alginate is added sterile distilled water heating for dissolving, be configured to the solution that concentration is 2%, to be cooled to adding step (2) gained material when 40 DEG C, abundant mixing, is then dropwise added dropwise in calcium chloride solution, leaves standstill crosslinked 1 hour, with 0.3mol/L glutaraldehyde solution process 1 hour, wash with the sodium chloride solution of 0.9% again, then solidify 24 hours at 4 DEG C, for subsequent use;

(4) chitosan is added water be mixed with the chitosan solution that concentration is 0.3%, then the coating process in chitosan solution of step (3) gained material was taken out after 0.5 hour, mix with leftover materials, add water and make material moisture reach 20%, granulation, to obtain final product.

The microbial fertilizer being rich in nucleic acid of the present invention is used for plant common agricultural experiment, selects 5 mu of planting bases, use fertilizer of the present invention; as experimental group, common fertilizer is executed in 5 mu of bases of control group routinely planting requirement, and other condition is identical; experiment proves; when the farm crop of experimental group plantation reach equal agricultural output again, greatly reduce Fertilizer application amount, raw material is easy to get simultaneously; raw materials cost is lower; save labour, protection of the environment, on year-on-year basis economic raising 23.8%.

Claims (2)

1. be rich in a microbial fertilizer for nucleic acid, it is characterized in that being made up of the raw material of following weight part:

Bacterium chaff 50-52, magnesium sulfate 2-3, quartz powder 6-7, oyster shell whiting 3-4, nucleic acid waste water 8-9, attapulgite 25-30, the phosphate buffered saline buffer 70-72 of azotobacter chroococcum 5-5.5, pH7.0, sodium alginate 5-6, calcium chloride 4-5, chitosan 2-3.

2. the preparation method being rich in the microbial fertilizer of nucleic acid according to claim 1, is characterized in that comprising the following steps:

(1) by expanded for bacterium chaff twin-screw extruder rear cooling crush, crossing aperture is the sieve of 0.25mm, continuous sterilization 1 hour at 121 DEG C, dries;

(2) phosphate buffered saline buffer of pH7.0 and step (1) gained material are mixed, access azotobacter chroococcum, cultivates 7 days in the constant temperature biochemical cultivation case at 28 DEG C;

(3) calcium chloride is added sterile distilled water and be configured to the solution that concentration is 4%, obtain calcium chloride solution; Sodium alginate is added sterile distilled water heating for dissolving, be configured to the solution that concentration is 2%, to be cooled to adding step (2) gained material when 40 DEG C, abundant mixing, is then dropwise added dropwise in calcium chloride solution, leaves standstill crosslinked 1-1.5 hour, with 0.3mol/L glutaraldehyde solution process 1-1.5 hour, wash with the sodium chloride solution of 0.9% again, then solidify 24 hours at 4 DEG C, for subsequent use;

(4) chitosan is added water be mixed with the chitosan solution that concentration is 0.3%, then the coating process in chitosan solution of step (3) gained material was taken out after 0.5 hour, mix with leftover materials, add water and make material moisture reach 20%, granulation, to obtain final product.