CN105560548A - Plant source compound preparation special for apostichopus japonicus reversed pond medicated bath - Google Patents
Plant source compound preparation special for apostichopus japonicus reversed pond medicated bath Download PDFInfo
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- CN105560548A CN105560548A CN201610022948.6A CN201610022948A CN105560548A CN 105560548 A CN105560548 A CN 105560548A CN 201610022948 A CN201610022948 A CN 201610022948A CN 105560548 A CN105560548 A CN 105560548A
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- radix
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- dipping
- morinae bulleyanae
- radix morinae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
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- Insects & Arthropods (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a plant source compound preparation special for an apostichopus japonicus reversed pond medicated bath. The plant source compound preparation is low in cost and convenient to use, resists stress reaction, restrains bacteria and is free of medicine residues. The preparation is prepared from, by mass, 40-50% of isomaltose hypgather, 9-11% of pericarpium granati, 9-11% of fructus schizandrae, 9-11% of radix scutellariae, 9-10% of radix angelicae dahuricae, 9-10% of earthworms, 4-5% of smoked plum and 1-2% of gallnut.
Description
Technical field
The present invention relates to a kind of Radix Morinae Bulleyanae dipping of falling pond preparation, especially a kind of with low cost, easy to use, alternative antibiotic, the compound botanical preparation being exclusively used in the Radix Morinae Bulleyanae dipping bath of falling pond strengthening Radix Morinae Bulleyanae immunity, alleviate stress.
Background technology
In order to provide good growing environment to culturing stichopus japonicus, prevent disease, Radix Morinae Bulleyanae to be fallen pond by usual needs every 15 ~ 20 days, pull out from Yuan Chizhong by Radix Morinae Bulleyanae, after dipping sterilization is concentrated to Radix Morinae Bulleyanae, then Radix Morinae Bulleyanae is moved to at the bottom of pool wall, pond and adherance etc. carry out in the culturing pool of thorough cleaning and sterilizing.Because pond easily causes Radix Morinae Bulleyanae stress, therefore dipping must adopt stimulates less preparation to Radix Morinae Bulleyanae.Although more existing Chinese herbal and crude drugs preparationses etc. prevent and treat Radix Morinae Bulleyanae disease non-harmful product at present, be all only limited to the control of disease in apostichopus japonicus culture process.As being applied to the Radix Morinae Bulleyanae dipping of falling pond, because its concentration is higher, Radix Morinae Bulleyanae is to the reason such as some Chinese herbal medicine is more responsive, cause stimulating comparatively greatly Radix Morinae Bulleyanae, Radix Morinae Bulleyanae not only can be caused to occur telling the stress such as intestinal, health contraction, and the normal growth of Radix Morinae Bulleyanae can be affected.For this reason, what the dipping of falling pond at present adopted is all antibiotic less to Radix Morinae Bulleyanae zest.Although antibiotic can kill part pathogenic bacterium, Reusability can cause Radix Morinae Bulleyanae drug disposition to remain and Resistant strain occurs, has both reduced the immunity of Radix Morinae Bulleyanae, and has directly endangered again the health of eater.Therefore, with the dipping of plant source preparation substitute antibiotics in the process of falling pond, be industry look forward to the difficult problem that can solve, be also avoid from source using antibiotic key link.
Summary of the invention
The present invention is the above-mentioned technical problem in order to solve existing for prior art, provides a kind of antibiotic with low cost, alternative, alleviates the stress brought in pond, strengthen the compound botanical preparation being exclusively used in the Radix Morinae Bulleyanae dipping bath of falling pond of Radix Morinae Bulleyanae immunity.
Technical solution of the present invention is: a kind of compound botanical preparation being exclusively used in the Radix Morinae Bulleyanae dipping of falling pond, it is characterized in that being made up of oligomeric isomaltose, Pericarpium Granati, Fructus Schisandrae Chinensis, Radix Scutellariae, the Radix Angelicae Dahuricae, Pheretima, Fructus Mume, Galla Chinensis, the mass percent of each raw material is as follows: oligomeric isomaltose 40 ~ 50%, Pericarpium Granati 9 ~ 11%, Fructus Schisandrae Chinensis 9 ~ 11%, Radix Scutellariae 9 ~ 11%, the Radix Angelicae Dahuricae 9 ~ 10%, Pheretima 9 ~ 10%, Fructus Mume 4 ~ 5%, Galla Chinensis 1 ~ 2%.
Instant component collocation is reasonable, synergism between each component, and tool has the following advantages:
(1) there is effect of antibacterial sterilization, alternative antibiotic dipping, avoid antibiotics residues and Resistant strain in Radix Morinae Bulleyanae body to occur;
(2) stress of Radix Morinae Bulleyanae in the process of falling pond is alleviated, phenomenons such as appearance activity behind pond is abnormal, health tightens, tell intestinal, change skin of avoiding Radix Morinae Bulleyanae to fall;
(3) immunity of Radix Morinae Bulleyanae can be improved, be conducive to resisting various virulence factor;
(4) degradable under field conditions (factors), on water body physical and chemical index without impact;
(5) addition is few, easy to use;
(6) raw material supply is stablized, with low cost.
Accompanying drawing explanation
Fig. 1 is experimental example gene involved in immunity expression schematic diagram of the present invention.
Detailed description of the invention
Embodiment 1:
The mass percent of each raw material is as follows: oligomeric isomaltose 50%, Pericarpium Granati 9%, Fructus Schisandrae Chinensis 9%, Radix Scutellariae 9%, the Radix Angelicae Dahuricae 9%, Pheretima 9%, Fructus Mume 4%, Galla Chinensis 1%.
Add 10 times of volume water after being mixed by raw material, slow fire boiling 30min after soaking 20min, eight layers of filtered through gauze collect medicinal liquid; Medicinal residues add 10 times of water again, slow fire boiling 30min, and eight layers of filtered through gauze collect medicinal liquid; Repeat again once, three gained medicinal liquids are merged and is concentrated into 200g/L, make water preparation.
Embodiment 2:
Oligomeric isomaltose 50%, Pericarpium Granati 9%, Fructus Schisandrae Chinensis 9%, Radix Scutellariae 9%, the Radix Angelicae Dahuricae 9%, Pheretima 9%, Fructus Mume 4%, Galla Chinensis 1%.
Each raw material powder is broken into 200 order powder, makes powder.
Embodiment 3:
The mass percent of each raw material is as follows: oligomeric isomaltose 40%, Pericarpium Granati 11%, Fructus Schisandrae Chinensis 11%, Radix Scutellariae 11%, the Radix Angelicae Dahuricae 10%, Pheretima 10%, Fructus Mume 5%, Galla Chinensis 2%.
Add 10 times of volume water after being mixed by raw material, slow fire boiling 30min after soaking 20min, eight layers of filtered through gauze collect medicinal liquid; Medicinal residues add 10 times of water again, slow fire boiling 30min, and eight layers of filtered through gauze collect medicinal liquid; Repeat again once, three gained medicinal liquids are merged and is concentrated into 200g/L, make water preparation.
Experimental example:
1. laboratory test:
Test is carried out in the plastic box of 50X30X20cm, and Radix Morinae Bulleyanae size is every 500 grams 200, and experimental group and matched group respectively arrange 3 repetitions.Experimental group shares 115 Radix Morinae Bulleyanaes, is to be added water by the water preparation of embodiment 1 to carry out concentrated dipping bath 15min by the concentration of 0.2g/L to Radix Morinae Bulleyanae; Matched group uses sand filter seawater dipping bath 15min, and other process is identical with experimental group.
90 Radix Morinae Bulleyanaes are assigned randomly in 3 plastic boxes by 1.1, normally feed 30 days, observe growing state.
Experimental result is as follows:
| Initial average weight (g) | Final average weight (g) | |
| Experimental group | 36.35 | 42.25 |
| Matched group | 36.59 | 42.41 |
Result shows: experimental group Radix Morinae Bulleyanae and matched group Radix Morinae Bulleyanae body weight there was no significant difference.
2. cytophagy activity test: get 4 Radix Morinae Bulleyanae Radix Morinae Bulleyanae injection carmine 200ul after medicinal liquid soaks, after 6h, get wherein 3 Radix Morinae Bulleyanae coelomic fluids, add the mixing of equivalent anticoagulant, counted under microscope coelomocyte sum and the cell quantity engulfed, often group chooses 9 visual field countings under mirror, and ensures the coelomocyte sum > 100 of counting, calculates phagocytic rate.
| Phagocytic rate (%) | |
| Experimental group | 0.3786 |
| Matched group | 0.3209 |
Result shows: through medicinal liquid soak after, experimental group coelomocyte of Apostichopus japonicus phagocytic rate higher than matched group, significant difference between two groups.
3. get 21 Radix Morinae Bulleyanaes respectively after dipping completes 10min, 30min, 1h, 4h, 12h and 24h relative quantification method measure 4 kinds of gene involved in immunity expressions.NADH is selected to be reference gene.
Result as shown in Figure 1.
Result shows, 4 kinds of gene involved in immunity present all at short notice first to rise and drop to stable trend afterwards, and peak value appears at 1h, illustrates that the present invention has the effect improving Radix Morinae Bulleyanae immunity.
2. industrial experimentation
2.1 experiments are implemented in Dalian Bao Fa precious marine product company limited.Radix Morinae Bulleyanae size is every 500 grams 1000, and 12.5kg stichopus japonicus seedling, experimental group and each 3 ponds of matched group are placed in every pond.Experimental group is that embodiment 1 water preparation is carried out concentrated dipping by the concentration of 0.2g/L to Radix Morinae Bulleyanae, matched group uses the ceftriaxone sodium of same concentrations to carry out dipping, put in cement culturing pool after each group of equal dipping 15min and normally raise, 12 days afterwards fall pond take weight result as following table, experimental group and matched group there was no significant difference.
2.2 second time are produced checking and are carried out in Dalian Bao Fa precious marine product company limited, and experimental group is this patent embodiment 1, embodiment 2 adds water water decoction dipping group, powder dipping group that 0.2g/L in proportion makes, matched group is isoconcentration ceftriaxone sodium dipping group.Wherein water decoction dipping group 20 culturing pools, powder dipping group 5 culturing pools, matched group 20 culturing pools.Stichopus japonicus is concentrated dipping 15min, put in culturing pool, observe Stichopus japonicus state.
Result:
(1) use embodiment of the present invention water decoction and powder to carry out the Stichopus japonicus energy of dipping comparatively by force, after splashing on mesh sheet, dispersion is very fast.
(2) Continuous Observation 20 days experimental grouies and matched group are all told intestinal, are changed the symptoms such as skin, ingest normal.
2.3 third times produced checking and carry out at Zhuanghe Xinhua aquatic farm.Experimental group use this patent embodiment 2,3 to add water powder dipping group, water decoction dipping group that 0.2g/L in proportion makes, matched group is ceftriaxone sodium+" pul is emerging " dipping group, wherein two experimental grouies and each 3 culturing pools of matched group.
Result:
(1) use water decoction of the present invention and powder to carry out the Stichopus japonicus energy of dipping comparatively by force, within 15min, gather plastic tub upper strata in a large number, matched group Stichopus japonicus, energy is relatively poor, and in 15min, most Stichopus japonicus is also piled up at plastic tub bottom.
(2) Continuous Observation 15 days experimental grouies and matched group are all told intestinal, are changed the symptoms such as skin, ingest normal.
The above results shows, the present invention effectively can alleviate the stress state of Radix Morinae Bulleyanae, suppress the pathogen such as vibrio active, certain Radix Morinae Bulleyanae immune level can be improved, prevent Radix Morinae Bulleyanae from telling intestinal, changing the disease generations such as skin, and Radix Morinae Bulleyanae active state is better than using antibiotic dipping effect, on Radix Morinae Bulleyanae growth without impact, can use by substitute antibiotics completely.
Claims (1)
1. one kind is exclusively used in the compound botanical preparation of the Radix Morinae Bulleyanae dipping of falling pond, it is characterized in that being made up of oligomeric isomaltose, Pericarpium Granati, Fructus Schisandrae Chinensis, Radix Scutellariae, the Radix Angelicae Dahuricae, Pheretima, Fructus Mume, Galla Chinensis, the mass percent of each raw material is as follows: oligomeric isomaltose 40 ~ 50%, Pericarpium Granati 9 ~ 11%, Fructus Schisandrae Chinensis 9 ~ 11%, Radix Scutellariae 9 ~ 11%, the Radix Angelicae Dahuricae 9 ~ 10%, Pheretima 9 ~ 10%, Fructus Mume 4 ~ 5%, Galla Chinensis 1 ~ 2%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610022948.6A CN105560548A (en) | 2016-01-14 | 2016-01-14 | Plant source compound preparation special for apostichopus japonicus reversed pond medicated bath |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610022948.6A CN105560548A (en) | 2016-01-14 | 2016-01-14 | Plant source compound preparation special for apostichopus japonicus reversed pond medicated bath |
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| CN105560548A true CN105560548A (en) | 2016-05-11 |
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| CN201610022948.6A Pending CN105560548A (en) | 2016-01-14 | 2016-01-14 | Plant source compound preparation special for apostichopus japonicus reversed pond medicated bath |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582662A (en) * | 2017-08-29 | 2018-01-16 | 大连海洋大学 | The quick Chinese herbal formula for repairing apostichopus japonicus body-wall skin tissue |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322760A (en) * | 2008-08-06 | 2008-12-17 | 大连水产学院 | Compound Chinese herbal medicine formulation for treating causative pathogen skin ulcerative syndrome of Apostichopus japonicus |
-
2016
- 2016-01-14 CN CN201610022948.6A patent/CN105560548A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101322760A (en) * | 2008-08-06 | 2008-12-17 | 大连水产学院 | Compound Chinese herbal medicine formulation for treating causative pathogen skin ulcerative syndrome of Apostichopus japonicus |
Non-Patent Citations (1)
| Title |
|---|
| 李华等: "几种中草药对海水养殖中常见病原菌的抗菌作用", 《大连海洋大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107582662A (en) * | 2017-08-29 | 2018-01-16 | 大连海洋大学 | The quick Chinese herbal formula for repairing apostichopus japonicus body-wall skin tissue |
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Application publication date: 20160511 |