CN107260815A - Prevent medicine that water mold is infected during different tooth schizothoracin hatch fish roe and preparation method thereof - Google Patents
Prevent medicine that water mold is infected during different tooth schizothoracin hatch fish roe and preparation method thereof Download PDFInfo
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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Abstract
The invention discloses a kind of medicine for preventing that water mold is infected during different tooth schizothoracin hatch fish roe, include the raw material of following weight proportion：0.6 1.8 parts of 0.7 2.4 parts of Chinese gall, 0.1 1.3 parts of rheum officinale, 0.1 2.1 parts of the fruit of a cubeb litsea tree, 0.6 1.8 parts of radix scutellariae and kuh-seng.The medicine of the present invention is efficient, safe, cheap, and water mold is infected during preventing different tooth schizothoracin hatch fish roe, and is had no toxic side effect.Present invention also offers the preparation method of the medicine.The present invention's prevents the medicine that water mold is infected during different tooth schizothoracin hatch fish roe to be made of Chinese medicine, and it has the advantages that efficient, safe, cheap, can effectively suppress the water mold during different tooth schizothoracin hatch fish roe and infect.
Description
Technical field
During different tooth schizothoracin hatch fish roe being prevented the present invention relates to fish culture disease prevention and cure field, particularly one kind
The medicine that water mold is infected.
Background technology
Different tooth schizothoracin quilt《Chinese species Red List in 2008》Within fish category in imminent danger, academy of agricultural sciences of area aquatic products is incited somebody to action
《Yarlung Zangbo River Main Commercial Fishes enhancement releasing technology and recruitment evaluation》Project is intended implementing ground as institute " 13 " planning
Great research project, as Yarlung Zangbo River Main Commercial Fishes, carry out different tooth schizothoracin artificial propagation has turned into inevitable.Different
In tooth schizothoracin artificial propagation hatching process, saprolegniasis harm is very serious, is to influence one of main factor of incubation rate.In ovum
In hatching process, infected for prevention water mold, generally employ a large amount of workman persons to choose dead ovum, expend substantial amounts of manpower and financial resources,
Cause heavy economic losses, find the method that preventing and treating water mold during hatch fish roe infects extremely urgent.
Prevent and treat fish-egg saprolegniasis using malachite green in the past, but malachite green is because with carcinogenic, teratogenesis, mutagenic
Danger, forbids using on aquatic products for 2002.At present, formalin, hydrogen peroxide, ozone etc. also be used to prevent and treat water mold in succession
Disease, but harm of the formalin to operating personnel is larger and environment can be adversely affected；Hydrogen peroxide use cost is higher；
Ozone is also required to corresponding preparation facilities, at the same using and accumulating it is all inconvenient, it is difficult to promote.Therefore, efficient, peace is still lacked at present
Entirely, cheap preventing and treating water mold medicine, it is difficult to the medicine vacuum filled up malachite green disabled and occurred.
Source is wide, with low cost because of it for Chinese herbal medicine, favored by numerous scientific research personnel the advantages of be not likely to produce drug resistance.Mesh
It is preceding also less using Chinese herbal treatment saprolegniasis in aquatic products clinical application.Carry out the research of screening of anti-saprolegnia Chinese herbal medicine not only
The clinical problem of saprolegniasis active drug shortage is advantageously accounted for, and is conducive to my area's medicament categories of increase to lay in and reduction water
Produce food safety risk.
The content of the invention
To solve problems of the prior art, it is an object of the invention to provide a kind of efficient, safe, cheap, it can prevent
Chinese medicine preparation that only water mold is infected during different tooth schizothoracin hatch fish roe and preparation method thereof.
To achieve the above object, the technical solution adopted by the present invention is：One kind prevents different tooth schizothoracin hatch fish roe process
The medicine that middle water mold is infected, includes the raw material of following weight proportion：0.7-2.4 parts of Chinese gall, 0.1-1.3 parts of rheum officinale, the fruit of a cubeb litsea tree
0.6-1.8 parts of 0.1-2.1 parts, 0.6-1.8 parts of radix scutellariae and kuh-seng.
Preferably, the raw material of following weight proportion is included：1.5 parts of Chinese gall, 0.4 part of rheum officinale, 0.6 part of the fruit of a cubeb litsea tree, radix scutellariae
1.5 parts, 1.2 parts of kuh-seng.
Source is wide, with low cost because of it for Chinese herbal medicine, favored by numerous scientific research personnel the advantages of be not likely to produce drug resistance.Mesh
It is preceding also less using Chinese herbal treatment saprolegniasis in aquatic products clinical application.Carry out the research of screening of anti-saprolegnia Chinese herbal medicine not only
The clinical problem of saprolegniasis active drug shortage is advantageously accounted for, and is conducive to my area's medicament categories of increase to lay in and reduction water
Produce food safety risk.
The present invention selects five kinds of Chinese medicines (Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, hardships using different tooth schizothoracin as research object
Ginseng) gradient experiment is set, from different fissure abdomen fish fertilized egg to incubation of membrane, soaked daily with without the Chinese medicine of species various concentrations
Bubble, calculates incubation rate, dead ovum rate, water mold fatal rate and other factors fatal rate, filters out in the special efficacy that prevention water mold is infected
Medicine and its concentration.The Chinese medicine of certain concentration is combined again, embryonated egg dipping experiment is carried out again, finally filtering out prevents different
The Chinese medicine preparation that water mold is infected during tooth schizothoracin hatch fish roe.
It is a kind of to prevent the preparation method of the medicine that water mold is infected during different tooth schizothoracin hatch fish roe, its step as described above
It is as follows：
A, Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng are weighed by proportioning；
B, the Chinese gall weighed, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng soaked respectively；
C, the Chinese gall by after immersion, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out first time decoction respectively；
D, the Chinese gall by after decoction, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out first time filtering respectively, obtain respectively
Decoction and solid medicinal material；
E, will filter for the first time after the obtained solid medicinal material of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng add water respectively
Second is carried out to decoct；
F, second is decocted after Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out second of filtering respectively, point
Qu get not decoction and solid medicinal material；
G, will second filter after the obtained solid medicinal material of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng add water respectively
Carry out third time decoction；
H, by third time decoct after Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out third time filtering respectively, point
Qu get not decoction and solid medicinal material；
I, three times are filtered after obtained whole decoctions of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng are fused to obtain finished product
Decoction.
Preferably, in stepb, Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng add the water immersion of 10 times of weight respectively,
Soak time is 0.8h-1.2h.
Plus the multiple dose water of material 10, medicine can be totally submerged, and active ingredient leaching rate is high.Soak 0.8h-1.2h,
The part that can be leached in active ingredient can completely be leached, overlong time active ingredient, which is leached, will not also increase, and immersion 0.8h-
1.2h can be by the scale precipitation on medicine.
Preferably, in step c, first time decocting time is 0.8h-1.2h, and decoction is decocted using big fire for the first time.
First time big fire decocting time is 0.8h-1.2h, can come out main most of extracts active ingredients.Time mistake
Long active ingredient can be destroyed.
Preferably, in step e, by the solid medicine of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng during second of decoction
Material adds the water of 10 times of weight respectively, and second of decocting time is 40min-50min, and decoction for the second time is used and is cooked by slow fire.
The time is cooked by slow fire for the second time for 40min-50min, it is therefore intended that ensure the feelings that principle active component is not destroyed
Under condition, the remaining extracts active ingredients of medicinal material are come out.
Preferably, in step g, by the solid medicine of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng when third time is decocted
Material adds the water of 10 times of weight respectively, and third time decocting time is 28min-32min, and decoction for the third time is used and is cooked by slow fire.
Third time is cooked by slow fire the time for 28min-32min, it is therefore intended that ensure the feelings that principle active component is not destroyed
Under condition, remaining extracts active ingredients are come out, and time unsuitable long, long waste of resource.
Preferably, in step i, finished product decoction is diluted with water, makes each drug concentration be respectively：Chinese gall
1.5g/L, rheum officinale 0.4g/L, fruit of a cubeb litsea tree 0.6g/L, radix scutellariae 1.5g/L and kuh-seng 1.2g/L.
The beneficial effects of the invention are as follows：
1st, from Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, five kinds of anti-water mold medicines of medicines making of kuh-seng, pure Chinese medicinal preparation, into
Sheet is low, has no toxic side effect, be not likely to produce drug resistance；
2nd, by scientific experiment method, picking out each drug concentration in rational material rate, the present invention is respectively：Five times
Have when sub- 1.5g/L, rheum officinale 0.4g/L, fruit of a cubeb litsea tree 0.6g/L, radix scutellariae 1.5g/L, kuh-seng 1.2g/L and preferably suppress water mold infection
Effect, can effectively reduce fatal rate of the water mold to fish-egg.
Embodiment
Embodiment
The present invention is that primary raw material is fabricated to the anti-of pure Chinese medicinal preparation from Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, kuh-seng
Water mold medicine, is rational proportion Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, the proportional amount of kuh-seng, the following experiment of design.
1st, test material
1.1 medicine
The medicines such as Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, kuh-seng, malachite green.
1.2 experiment material
Different fissure abdomen fish fertilized egg, purchase parent population hasten parturition artificial insemination acquisition.
1.3 test equipment
Big basin 27, incubation plate 27, small-sized pump of supplementing nutrition, anatomical lens, temperature agent, dissolved oxygen meter, ammonia nitrogen kit, nitrous examination
Agent box, title and electronic balance.
2nd, influence of the single medicinal material to the lethal fish-egg number of water mold
The preparation of 2.1 single decoctions
Weigh Chinese medicine Chinese gall in table 1, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae, kuh-seng respectively, respectively plus 3 times of amounts water immersion 1h,
1h is decocted, decoction is filtered out；Decoction 45min is added water, decoction is filtered out；Decoction 30min is added water, merges 3 filtrates, it is dense by table 1
Shorten the decoction of various concentrations gradient into.
2.2 experiment packets and processing
Parent population is bought, acquisition embryonated egg of hastening parturition.Incubating oosperm to primitive gut early stage starts to calculate rate of fertilization.It will be fertilized good
Embryonated egg be randomly divided into 27 groups, every group of 3 repetitions each repeat 500 ovum.If a blank control group is (without any medicine
Immersion is steeped) and a positive controls (daily with 0.2g/L malachite greens immersion 35min), remaining test group is respectively according to table 1
Handled, 35min is soaked daily.Daily record water temperature, hold oxygen, ammonia nitrogen, nitrous, calculate water mold lethal fish-egg number, other because
The lethal fish-egg number of element.Water temperature is controlled at 11-18 DEG C, holds oxygen control in more than 6.0mg/L.Until fry membrane.It is grouped according to the form below
Carry out：
Each test group of table 1 is grouped situation
3rd, influence of the Chinese medicine compound prescription to the lethal fish-egg number of water mold
According to influence result of the single medicinal material to the lethal fish-egg number of water mold, select the best single medicinal material concentration of effect and enter
Row combination, while setting blank group, positive drug group (malachite green) soaks 35min daily.Water temperature is recorded daily, hold oxygen, ammonia
Nitrogen, nitrous, calculate the lethal fish-egg number of water mold, the lethal fish-egg number of other factors.Water temperature is controlled at 11-18 DEG C, is held oxygen control and is existed
6.0mg/L more than.Until fry membrane.
4th, the statistics of seedling incubation rate
In process of the test, water mold is calculated daily and causes fish-egg number, the lethal fish-egg number of other factors, hatching is counted after off-test
Rate, dead ovum rate, water mold fatal rate, other factors fatal rate.
Incubation rate=hatching fry number/hatching fish-egg sum * 100%
Dead ovum rate=dead ovum number/hatching fish-egg sum * 100%
The lethal ovum number of water mold fatal rate=water mold/hatching fish-egg sum * 100%
The lethal ovum number of other factors fatal rate=other factors/hatching fish-egg sum * 100%
5th, result of the test
Influence of 5.1 single medicinal materials to the lethal fish-egg number of water mold
It can be seen from result of the test：1.5g/L Chinese galls, 0.4g/L rheum officinales, the 0.6g/L fruits of a cubeb litsea tree, 1.5g/L radix scutellariaes,
The single medicinal material of 1.2g/L kuh-sengs has preferable effect to reducing the lethal fish-egg of water mold.
Influence of the single medicinal material of table 2 to the lethal fish-egg number of water mold
Note：The identical lowercase of same column represents that difference is not notable (P ＞ 0.05) without letter, different lowercase letters
Significant difference (P ＜ 0.05).
The influence that 5.2 different pharmaceuticals are infected to water mold during hatch fish roe
The result of influence according to single medicinal material to the lethal fish-egg number of water mold can just think that drug concentration is：Chinese gall 1.5g/
Suppress water mold effect when L, rheum officinale 0.4g/L, fruit of a cubeb litsea tree 0.6g/L, radix scutellariae 1.5g/L, kuh-seng 1.2g/L preferably, so from phase
The medicine of concentration is answered to constitute Chinese medicine preparation, result of the test shows：Compared with blank group, Chinese medicine preparation group fish-egg water mold fatal rate pole
Significantly reduce (P ＜ 0.01), and there is no significant difference with malachite green group.Malachite green is generally acknowledged treatment water mold special efficacy
Medicine, but banning drugs have been listed in, this experiment is intended to find malachite green substitute.
The influence that the different pharmaceutical of table 3 is infected to water mold during hatch fish roe
Note：The identical lowercase of same column represents that difference is not notable (P ＞ 0.05) without letter, different lowercase letters
Significant difference (P ＜ 0.05).
6th, conclusion
When each drug concentration is respectively in Chinese medicine preparation：Chinese gall 1.5g/L, rheum officinale 0.4g/L, fruit of a cubeb litsea tree 0.6g/L, Huang
Play the role of preferably to suppress water mold infection when a kind of reed mentioned in ancient books 1.5g/L, kuh-seng 1.2g/L, can effectively reduce water mold to the lethal of fish-egg
Rate.
Embodiment described above only expresses the embodiment of the present invention, and it describes more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (8)
1. a kind of medicine for preventing that water mold is infected during different tooth schizothoracin hatch fish roe, it is characterised in that including following weight
The raw material of proportioning：0.7-2.4 parts of Chinese gall, 0.1-1.3 parts of rheum officinale, 0.1-2.1 parts of the fruit of a cubeb litsea tree, 0.6-1.8 parts of radix scutellariae and kuh-seng
0.6-1.8 parts.
2. the medicine that water mold is infected during different tooth schizothoracin hatch fish roe is prevented according to claim 1, it is characterised in that
Include the raw material of following weight proportion：1.5 parts of Chinese gall, 0.4 part of rheum officinale, 0.6 part of the fruit of a cubeb litsea tree, 1.5 parts of radix scutellariae and kuh-seng 1.2
Part.
3. a kind of prevent the preparation of the medicine that water mold is infected during different tooth schizothoracin hatch fish roe as claimed in claim 1 or 2
Method, it is characterised in that step is as follows：
A, Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng are weighed by proportioning；
B, the Chinese gall weighed, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng soaked respectively；
C, the Chinese gall by after immersion, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out first time decoction respectively；
D, the Chinese gall by after decoction, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out first time filtering respectively, and decoction is obtained respectively
With solid medicinal material；
E, will filter for the first time after the obtained solid medicinal material of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng add water respectively progress
Second of decoction；
F, second is decocted after Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out second of filtering respectively, take respectively
Obtain decoction and solid medicinal material；
G, will second filter after the obtained solid medicinal material of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng add water respectively progress
Third time is decocted；
H, by third time decoct after Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng carry out third time filtering respectively, take respectively
Obtain decoction and solid medicinal material；
I, three times are filtered after obtained whole decoctions of Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, radix scutellariae and kuh-seng are fused to obtain finished medicines
Liquid.
4. preparation method according to claim 3, it is characterised in that in stepb, Chinese gall, rheum officinale, the fruit of a cubeb litsea tree, Huang
A kind of reed mentioned in ancient books and kuh-seng add the water immersion of 10 times of weight respectively, and soak time is 0.8h-1.2h.
5. preparation method according to claim 4, it is characterised in that in step c, first time decocting time is 0.8h-
1.2h, and decoction is decocted using big fire for the first time.
6. preparation method according to claim 5, it is characterised in that in step e, when decocting for second by Chinese gall,
Rheum officinale, the fruit of a cubeb litsea tree, the solid medicinal material of radix scutellariae and kuh-seng add the water of 10 times of weight respectively, and second of decocting time is 40min-
50min, and second decoct using being cooked by slow fire.
7. preparation method according to claim 6, it is characterised in that in step g, when third time is decocted by Chinese gall,
Rheum officinale, the fruit of a cubeb litsea tree, the solid medicinal material of radix scutellariae and kuh-seng add the water of 10 times of weight respectively, and third time decocting time is 28min-
32min, and third time decocted using being cooked by slow fire.
8. preparation method according to claim 7, it is characterised in that in step i, adds water dilute to the progress of finished product decoction
Release, make each drug concentration be respectively：Chinese gall 1.5g/L, rheum officinale 0.4g/L, fruit of a cubeb litsea tree 0.6g/L, radix scutellariae 1.5g/L and kuh-seng
1.2g/L。
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108635409A (en) * | 2018-06-25 | 2018-10-12 | 四川农业大学 | It is a kind of to be used to treat Magnolia cortex P.E powder of saprolegniasis and preparation method thereof |
| CN112691184A (en) * | 2020-12-31 | 2021-04-23 | 西藏自治区农牧科学院水产科学研究所 | Bacteriostatic composition for inhibiting saprolegnia and preparation method thereof |
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| CN102283905A (en) * | 2011-07-08 | 2011-12-21 | 北京市水产技术推广站 | Chinese herbal medicinal compound preparation for preventing and treating fish saprolegniasis and application thereof |
| CN103109875A (en) * | 2013-02-26 | 2013-05-22 | 福建师范大学 | Method for preparing drug for preventing and treating freshwater fish disease from litsea cubeba essential oil and dithiocyano-methane |
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| CN102283905A (en) * | 2011-07-08 | 2011-12-21 | 北京市水产技术推广站 | Chinese herbal medicinal compound preparation for preventing and treating fish saprolegniasis and application thereof |
| CN103109875A (en) * | 2013-02-26 | 2013-05-22 | 福建师范大学 | Method for preparing drug for preventing and treating freshwater fish disease from litsea cubeba essential oil and dithiocyano-methane |
Non-Patent Citations (2)
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| NGUYEN HV: "Antibacterial activity of Litsea cubeba (Lauraceae, May Chang) and its effects on the biological response of common carp Cyprinus carpio challenged with Aeromonas hydrophila.", 《J APPL MICROBIOL.》 * |
| 李川: "大鲵水霉病中草药治疗试验", 《河北渔业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108635409A (en) * | 2018-06-25 | 2018-10-12 | 四川农业大学 | It is a kind of to be used to treat Magnolia cortex P.E powder of saprolegniasis and preparation method thereof |
| CN112691184A (en) * | 2020-12-31 | 2021-04-23 | 西藏自治区农牧科学院水产科学研究所 | Bacteriostatic composition for inhibiting saprolegnia and preparation method thereof |
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| CN107260815B (en) | 2020-07-03 |
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