CN105284937B - A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application - Google Patents
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application Download PDFInfo
- Publication number
- CN105284937B CN105284937B CN201510860153.8A CN201510860153A CN105284937B CN 105284937 B CN105284937 B CN 105284937B CN 201510860153 A CN201510860153 A CN 201510860153A CN 105284937 B CN105284937 B CN 105284937B
- Authority
- CN
- China
- Prior art keywords
- radix angelicae
- angelicae sinensis
- fiber crops
- extract
- sinensis fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, and it is the extract of corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash.The present invention screens the activity of the anti-Radix Angelicae Sinensis fiber crops stomatosis of tens kinds of Chinese medicines by many experiments, it is preferred that go out the 4 taste Chinese medicines (corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash) with obvious anti-Radix Angelicae Sinensis fiber crops stomatosis activity, the present invention has screened the activity of the anti-Radix Angelicae Sinensis fiber crops stomatosis of different solvents betelnut extract simultaneously, as a result show, betel nut water extract has the activity of best anti-Radix Angelicae Sinensis fiber crops stomatosis.The present invention makes full use of natural resources of Chinese medicinal materials; it is preferred that corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash are raw material; the extract being prepared can be used for the biological pesticide for preparing green, efficient, less toxic anti-Radix Angelicae Sinensis fiber crops stomatosis, has important environmental protection meaning and economic value, is worthy of promotion and application.
Description
Technical field
The present invention relates to a kind of Chinese medical extract, and in particular to a kind of Chinese medical extract with anti-Radix Angelicae Sinensis fiber crops stomatosis activity
And its application.
Background technology
Radix Angelicae Sinensis (Angelica sinensis) is herbaceos perennial, belongs to samphire, is used as medicine with root, medicinal to go through
History is long, is China's rare traditional Chinese medicine, is known as the title of ten side's rules for doing division with a one-digit divisor on the abacus in TCM Gynecology clinic.Radix Angelicae Sinensis is sweet, pungent, warm, Return liver,
The heart, the spleen channel, have replenishing and activating blood, menstruction regulating and pain relieving, the effect of relaxing bowel.Volatile oil and water soluble ingredient are mainly contained, clinically
It is mainly used in the illnesss such as blood deficiency chlorosis, dizziness palpitaition, irregular menstruation, closed dysmenorrhea, asthenia cold abdominalgia, dry constipation of intestines, arthralgia pain due to rheumatism
Treatment.Radix Angelicae Sinensis main product has Min to return (Gansu Minxian County), cloud returns (cloud in China Gansu, Yunnan, Sichuan, Shanxi, Hubei and other places
South), point in (Sichuan) is returned in river.
In the main producing region of Radix Angelicae Sinensis medicinal material, the numb stomatosis incidence of disease is higher, it has also become the Major Diseases in Radix Angelicae Sinensis production of crude drugs, leads
Cause Radix Angelicae Sinensis medical material quanlity inferior, the significantly underproduction.The field experiment investigation in former years finds that Radix Angelicae Sinensis fiber crops stomatosis Field diseases are generally
84.8%, sick index is 56.4%, serious up to 100%.Radix Angelicae Sinensis fiber crops stomatosis occurs mainly in " patent medicine phase ", and symptom is in diseased plant
Aerial part unobvious, are mainly manifested in root.Root browning, become fiber crops, breach, sponge chaff corruption shape is presented in its cortex.Modern times grind
Study carefully and show, numb stomatosis is as a kind of insect pest caused by rot stem nematodes.
The prevention and controls of Radix Angelicae Sinensis fiber crops stomatosis mainly include cultural control, physical control and chemical prevention etc. at present, but
Mainly prevented and treated in actual production using chemical pesticide, wherein organophosphorus insecticides, such as legendary god of farming's pellet, phonamiphos, gram line pellet
Etc. the preventing and treating for being widely used in numb stomatosis, as a result cause medicinal material Pesticide Residues too high, environmental pollution is serious.In addition, these medicines
Long-term a large amount of uses of agent cause nematode to produce resistance to agricultural chemicals, are allowed to be more difficult to wipe out and treat insect pests and plant diseases, prevention effect is not good enough.
Therefore, a kind of efficient, low toxicity, low-residual, inexpensive and convenient use bion nematicide are developed
As it is current there is an urgent need to.A series of active principle of the invention by screening anti-nematode from Chinese medicinal material extracts, to
Reach the purpose for safely, effectively preventing and treating Radix Angelicae Sinensis fiber crops stomatosis.
The content of the invention
Goal of the invention：It is an object of the invention to make full use of natural resources of Chinese medicinal materials, anti-Radix Angelicae Sinensis fiber crops mouth is screened by many experiments
The Chinese medicine and its extract of sick activity, the present invention is green using corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash as traditional Chinese medicinal material raw materials, into
This is low, and activity is strong, has important environmental protection meaning.
Technical scheme：In order to realize the above object the technical scheme that the present invention takes is：
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, it is the extract of corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash.
Preferably, the Chinese medical extract of above-described anti-Radix Angelicae Sinensis fiber crops stomatosis, it is by corter pseudolaricis, kuh-seng, betel nut
Or Chinese prickly ash is crude drug, is obtained using the extraction of petroleum ether, ethyl acetate, n-butanol, ethanol or water.
As more preferred scheme, the Chinese medical extract of above-described anti-Radix Angelicae Sinensis fiber crops stomatosis, extracting method carries for backflow
Follow the example of, ultrasonic extraction, continuous circumfluence extraction method, extraction time is 1~3 time, every time 0.5~2 hour.
The Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis of the present invention answering in anti-Radix Angelicae Sinensis fiber crops stomatosis pesticidal preparations are prepared
With.
Anti-rot Ditylenchus dipsaci screening active ingredients experiment
First, the Chinese medicine screening experiment of anti-rot Ditylenchus dipsaci activity
1st, experimental method
Method of directly tagging is co-cultured using dimethyl sulfoxide (DMSO) (DMSO) solution and nematode solution of different Chinese medicinal material extracts.
Each medicinal material alcohol reflux extract 10mg is accurately weighed, is dissolved in DMSO sample mother liquor is made respectively.During experiment with
The test solution that DMSO is diluted to final concentration of 500 μ g/mL carries out contact toxicity measure.Draw appropriate nematode suspension and be injected separately into 96
In the sample well of porocyte culture plates, nematode about 20~40, supplement sterilized water to the μ L of cumulative volume 300 are contained per hole, often processing sets 3
Group repeats, and the DMSO and sterilized water to add respective concentration are used as control respectively.25 DEG C respectively cultivate the laggard line worm of 3d, 7d it is dead
Die the statistics with survival condition.Calculation formula is as follows：
Nemic death rate (%)=(verge of death borer population/confession examination nematode number) × 100%
Nematode corrected mortality (%)=(treatment group nemic death rate-control group nemic death rate)/(1-control group is dead
Rate) × 100%
2nd, experimental result
By investigating tens kinds of different Chinese medicine alcohol reflux extracts to the situations of tagging of rot stem nematodes, as a result table
It is bright, co-culture 3d after, 4 kinds of medicinal substances extracts (corter pseudolaricis, kuh-seng, betel nut, Chinese prickly ash) to the fatal rate of nematode more than 80%, wherein
Betelnut extract is best to the lethal effect of nematode, and fatal rate is up to 85.8%；After co-culturing 7 days, 4 kinds of medicinal substances extracts are to line
The fatal rate and correction fatal rate of worm are more than 90%, the action of contace poison significant effect to nematode, as shown in Figure 1.
2nd, the anti-rot Ditylenchus dipsaci screening active ingredients experiment of betel nut the different extracted parts
Based on the studies above result, the present invention carries out Bin using different solvents such as petroleum ether, ethyl acetate, n-butanol, water
Bulky extraction, each extract part is concentrated to dryness, and is dissolved in respectively and final concentration of 500 μ g/mL test solution is made in DMSO is entered line
Worm contact toxicity detects, the results showed that (such as Fig. 2), betel nut water extract part are most strong to the lethal effect of nematode, are co-culturing the 3rd
It when, fatal rate has just reached 95.8%.
In addition, the present invention has also investigated action of contace poison of the betel nut water extract of various concentrations to Radix Angelicae Sinensis fiber crops stomatosis nematode, such as
Fig. 3 test result indicates that, with the raising of betel nut water extract concentration, its action of contace poison enhancing, when the concentration of betel nut water extract reaches
To 125 μ g/mL, when being co-cultured 5 days with Radix Angelicae Sinensis fiber crops stomatosis nematode, its fatal rate to Radix Angelicae Sinensis fiber crops stomatosis nematode can reach
81.9%, its fatal rate to Radix Angelicae Sinensis fiber crops stomatosis nematode may be up to 97.2% when co-culturing 7 days, show good anti-Radix Angelicae Sinensis
The activity of numb stomatosis.
Beneficial effect：The Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis provided by the invention has advantages below：
The present invention screens the activity of the anti-Radix Angelicae Sinensis fiber crops stomatosis of tens kinds of Chinese medicines by many experiments, preferably goes out to have obvious anti-
The 4 taste Chinese medicines (corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash) of Radix Angelicae Sinensis fiber crops stomatosis activity, while the present invention has screened different solvents Bin
The activity of the anti-Radix Angelicae Sinensis fiber crops stomatosis of bulky extract, the results showed that, betel nut water extract has the work of best anti-Radix Angelicae Sinensis fiber crops stomatosis
Property.The present invention makes full use of natural resources of Chinese medicinal materials, and preferably corter pseudolaricis, kuh-seng, betel nut or Chinese prickly ash is raw material, and the extract being prepared can
For preparing the biological pesticide of green, efficient, less toxic anti-Radix Angelicae Sinensis fiber crops stomatosis, have important environmental protection meaning and
Economic value, it is worthy of promotion and application.
Brief description of the drawings
Fig. 1 is block diagram of four kinds of medicinal material ethanol extracts to rot stem nematodes action of contace poison.
Fig. 2 is block diagram of the betel nut the different extracted parts to rot stem nematodes action of contace poison.
Fig. 3 is block diagram of the water extract to rot stem nematodes action of contace poison of betel nut various concentrations.
Embodiment
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments be merely to illustrate the present invention and
Limitation the scope of the present invention is not used in, after the present invention has been read, various of equal value shapes of the those skilled in the art to the present invention
The modification of formula falls within the application appended claims limited range.
Embodiment 1
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, betel nut crude drug is taken, add betel nut medicinal material 10 times of volumes of weight
Water, refluxing extraction 3 times, 1 hour every time, it is concentrated to give the active betelnut extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 2
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, betel nut crude drug is taken, add the second of betel nut medicinal material 8 times of volumes of weight
Acetoacetic ester, refluxing extraction 2 times, 2 hours every time, it is concentrated to give the active betelnut extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 3
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, betel nut crude drug is taken, add betel nut medicinal material 12 times of volumes of weight
N-butanol, continuous circumfluence extraction 3 times, 1 hour every time, it is concentrated to give the active betelnut extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 4
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, betel nut crude drug is taken, add the stone of betel nut medicinal material 8 times of volumes of weight
Oily ether, continuous circumfluence extraction 2 times, 2 hours every time, it is concentrated to give the active betelnut extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 5
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, corter pseudolaricis crude drug is taken, add corter pseudolaricis medicinal material 10 times of bodies of weight
Long-pending water, refluxing extraction 3 times, 1 hour every time, it is concentrated to give the active corter pseudolaricis extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 6
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, kuh-seng crude drug is taken, add Sophora flavescens 10 times of volumes of weight
Water, refluxing extraction 2 times, 2 hours every time, it is concentrated to give the active shrubby sophora extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
Embodiment 7
A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis, Chinese prickly ash crude drug is taken, add Chinese prickly ash medicinal material 10 times of volumes of weight
Petroleum ether, refluxing extraction 3 times, 1 hour every time, it is concentrated to give the active pepper extract of anti-Radix Angelicae Sinensis fiber crops stomatosis.
The Chinese medical extract for the anti-Radix Angelicae Sinensis fiber crops stomatosis being prepared using the embodiment of the present invention 1~7, add auxiliary agent and breast is made
The agricultural formulations such as oil, suspending agent, wettable powder, pulvis, granula, aqua, mother liquor or female powder.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (1)
1. application of the corter pseudolaricis extract in anti-Radix Angelicae Sinensis fiber crops stomatosis pesticidal preparations are prepared, described corter pseudolaricis extract is second
Alcohol reflux extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510860153.8A CN105284937B (en) | 2015-11-30 | 2015-11-30 | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510860153.8A CN105284937B (en) | 2015-11-30 | 2015-11-30 | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105284937A CN105284937A (en) | 2016-02-03 |
| CN105284937B true CN105284937B (en) | 2018-03-09 |
Family
ID=55183451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510860153.8A Expired - Fee Related CN105284937B (en) | 2015-11-30 | 2015-11-30 | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105284937B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106105613A (en) * | 2016-06-24 | 2016-11-16 | 渭源县鹿鸣现代农业专业合作社 | A kind of prevention and controls of Radix Angelicae Sinensis fiber crops stomatosis |
| CN113439764B (en) * | 2021-08-20 | 2022-08-30 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Composition for green prevention and control of angelica sinensis mouth disease and method and application thereof |
| CN117562086B (en) * | 2024-01-15 | 2024-03-19 | 西南林业大学 | Pesticide for preventing and controlling spodoptera frugiperda |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007132224A2 (en) * | 2006-05-12 | 2007-11-22 | Plant Impact Plc. | Nematicidal composition |
| CN103583606A (en) * | 2013-11-21 | 2014-02-19 | 成都新朝阳作物科学有限公司 | Root-knot nematode control agent as well as preparing method and application thereof |
| CN104845624A (en) * | 2015-04-20 | 2015-08-19 | 山西年马肥业有限公司 | Natural plant biological composite nutrient solution for killing nematode and preparation method thereof |
| CN104855442A (en) * | 2015-05-15 | 2015-08-26 | 江苏徐淮地区淮阴农业科学研究所 | Medical fertilizer for preventing and controlling nematodes and application method |
-
2015
- 2015-11-30 CN CN201510860153.8A patent/CN105284937B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007132224A2 (en) * | 2006-05-12 | 2007-11-22 | Plant Impact Plc. | Nematicidal composition |
| CN103583606A (en) * | 2013-11-21 | 2014-02-19 | 成都新朝阳作物科学有限公司 | Root-knot nematode control agent as well as preparing method and application thereof |
| CN104845624A (en) * | 2015-04-20 | 2015-08-19 | 山西年马肥业有限公司 | Natural plant biological composite nutrient solution for killing nematode and preparation method thereof |
| CN104855442A (en) * | 2015-05-15 | 2015-08-26 | 江苏徐淮地区淮阴农业科学研究所 | Medical fertilizer for preventing and controlling nematodes and application method |
Non-Patent Citations (7)
| Title |
|---|
| "22种中草药提取物杀根结线虫活性";刘晟 等;《农药》;20090810;第48卷(第8期);第599页2.2-2.3小节、表1，第600-601页表3-4 * |
| "8种植物杀灭鱼类指环虫的活性部位研究";王高学 等;《西北农林科技大学学报（自然科学版）》;20080310;第36卷(第3期);第65页第1.2小节 * |
| "Insecticidal Activity of Five Chinese Medicinal Plants Against Plutella xylostella L. (Lepidoptera: Plutellidae)";Patcharaporn Vanichpakorn;《中国博士学位论文全文数据库 农业科技辑》;20100815(第08期);中文摘要 * |
| "土荆皮抗真菌化学成分研究";徐云辉 等;《中草药》;20120212;第43卷(第2期);第221页第2节 * |
| "植物提取物对卫矛尺蠖卵的毒杀效果";王元兰 等;《安徽农业科学》;20050130;第33卷(第1期);第37页1.2.1小节 * |
| "水提取法制备苦参配方颗粒的工艺研究";巨建云 等;《中药材》;20091025;第32卷(第10期);第1625页2.1小节、表1 * |
| "花椒提取物的杀虫活性研究";寇芸芸 等;《食品研究与开发》;20150410;第36卷(第7期);第2页1.5.1小节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105284937A (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104705354B (en) | A kind of gardens plant insecticide | |
| CN101690767B (en) | Compound pesticide for fishing and preparation method and application thereof | |
| CN105284937B (en) | A kind of Chinese medical extract of anti-Radix Angelicae Sinensis fiber crops stomatosis and its application | |
| CN101965848A (en) | Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof | |
| CN105475385B (en) | A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry | |
| CN109479904A (en) | Middle mountain China fir essential oil and its application in agricultural insect pests control | |
| CN105360168A (en) | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus | |
| CN104705349B (en) | The preparation at white lotus parthenium extract different activities position and its application as acaricidal activity material | |
| CN101069508A (en) | Farm chemical composition, preparing method and use | |
| CN103860663B (en) | A kind of control mycotic Chinese medicine preparation of aquiculture animal and preparation method | |
| CN101986844B (en) | Application of asarum extract in preventing and controlling agricultural mites | |
| CN109452320A (en) | The purposes of Fructus Caryophylli volatile oil | |
| CN103548884B (en) | Chinese pistache kills Tetranychus cinnabarinus agent and preparation method thereof | |
| CN106359458A (en) | Flos Inulae-containing insecticidal composition | |
| CN103315008B (en) | Preparation method of Suaeda salsa used as agricultural pesticide and application | |
| CN102988462B (en) | Chinese medicinal preparation for controlling parasites of aquatic animal as well as preparation method and application thereof | |
| CN103548886B (en) | Use of Ferula bungeanaemulsion oil or microemulsion as insecticide and acaricide | |
| CN101919416B (en) | Method for extracting insecticidal active material from traditional Chinese medicine feather cockscomb and application thereof | |
| CN105724450B (en) | A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry | |
| CN101396024A (en) | Dendrolimus punctatus cytoplasmic polyhedrosis virus(dpcpv) bacillus thuringiensis wettable powder and preparation method thereof | |
| CN105767024A (en) | Plant-sourced acaricide containing acalypha australis extract and preparation method thereof | |
| CN101584349A (en) | Castor bean extractive and preparation method and application thereof | |
| CN108295119A (en) | Special pest repellant of a kind of largemouth bass cultivation and preparation method thereof | |
| CN106305813A (en) | Insecticidal composition containing Polygonum hydropiper | |
| CN106070350A (en) | A kind of Pesticidal combination containing Radix Sophorae Flavescentis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180309 Termination date: 20191130 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |