CN110693736A

CN110693736A - Pure natural plant essential oil sterilization hand sanitizer and preparation method thereof

Info

Publication number: CN110693736A
Application number: CN201810749251.8A
Authority: CN
Inventors: 许旭东; 朱霄伟; 马国需; 朱乃亮; 王滨
Original assignee: Triangle Mountain Beijing Biotechnology Co ltd; Xinyang Triangle Mountain Biotechnology Co ltd; Institute of Medicinal Plant Development of CAMS and PUMC
Current assignee: Triangle Mountain Beijing Biotechnology Co ltd; Xinyang Triangle Mountain Biotechnology Co ltd; Institute of Medicinal Plant Development of CAMS and PUMC
Priority date: 2018-07-10
Filing date: 2018-07-10
Publication date: 2020-01-17
Anticipated expiration: 2038-07-10
Also published as: CN110693736B

Abstract

A pure natural plant essential oil sterilization hand sanitizer and a preparation method thereof. The hand sanitizer comprises: 1.0-1.5% of rhizoma atractylodis volatile oil, 0.8-1.2% of cassia twig volatile oil, 0.1-0.2% of sodium chloride, 0.5-0.7% of hydroxymethyl propyl cellulose, 0.1-0.2% of disodium EDTA (ethylene diamine tetraacetic acid), 6-7% of sodium methyl cocoyl taurate, 3-4% of cocamide DEA, 8-10% of cocamidopropyl betaine, 3-4% of sodium lauryl ether sulfate and 8-10% of glycerol. The hand sanitizer product is non-toxic, harmless, green and environment-friendly, has no stimulation and side effect on a human body, and has a quick and lasting killing effect on common harmful bacteria such as staphylococcus aureus, candida albicans, escherichia coli, streptococcus and the like.

Description

Pure natural plant essential oil sterilization hand sanitizer and preparation method thereof

Technical Field

The invention relates to a hand sanitizer prepared from pure natural plant extract or essential oil.

Background

Along with the improvement of living standard of people and the more and more extensive demand on outdoor health activities. Many people utilize holidays and are exposed to the elements. Outdoor diet becomes an inevitable behavior. In view of the limitations of outdoor conditions, it is sometimes difficult to find an adequate and clean water source, and eating activities often have hygienic concerns. Therefore, various hand washing solutions have been produced. The main function of the hand sanitizer is to clean and protect skin, and some specific components can perform the functions of disinfection and sterilization.

At present, the domestic known hand sanitizer mostly takes triclosan and trichlorocarbanilide as main components, wherein the triclosan and the trichlorocarbanilide are broad-spectrum antibacterial agents and have high-efficiency inhibition and sterilization effects on gram-positive bacteria, gram-negative bacteria, fungi, yeasts and the like. However, the two main components not only have the risk of destroying the immune system of the human body, but also are difficult to degrade in the natural environment, and easily cause pollution to the environment. Therefore, the research and development of the bacteriostatic, degerming or bactericidal hand sanitizer which is natural, harmless to human bodies, environment-friendly and pollution-free is particularly important.

Disclosure of Invention

The invention aims to provide a hand sanitizer with sterilization, disinfection and mild decontamination.

According to a first aspect of the present invention, there is provided a hand sanitizer comprising: 1.0-1.5% of rhizoma atractylodis volatile oil, 0.8-1.2% of cassia twig volatile oil, 0.1-0.2% of sodium chloride, 0.5-0.7% of hydroxymethyl propyl cellulose, 0.1-0.2% of disodium EDTA (ethylene diamine tetraacetic acid), 6-7% of sodium methyl cocoyl taurate, 3-4% of cocamide DEA (coconut diethanolamide), 8-10% of cocamidopropyl betaine, 3-4% of sodium lauryl ether sulfate and 8-10% of glycerol.

According to a preferred embodiment of the present invention, the hand sanitizer may comprise: 1.0 to 1.2 percent of rhizoma atractylodis volatile oil, 0.8 to 1.0 percent of cassia twig volatile oil, 0.1 to 0.2 percent of sodium chloride, 0.5 to 0.7 percent of hydroxymethyl propyl cellulose, 0.1 to 0.2 percent of disodium EDTA, 6 to 7 percent of methyl cocoyl taurate, 3 to 4 percent of cocamide DEA, 8 to 10 percent of cocamidopropyl betaine, 3 to 4 percent of sodium lauryl ether sulfate and 8 to 10 percent of glycerol.

According to a further preferred embodiment of the present invention, the hand sanitizer may comprise: rhizoma atractylodis volatile oil: 1.0-1.5% of cassia twig volatile oil: 0.8% -1.2%, garden balsam stem oil: 0.1 to 0.3 percent of sodium chloride, 0.1 to 0.2 percent of sodium chloride, 0.5 to 0.7 percent of hydroxymethyl propyl cellulose, 0.1 to 0.2 percent of EDTA disodium, 6 to 7 percent of methyl cocoyl sodium taurate, 3 to 4 percent of cocamide DEA, 8 to 10 percent of cocamidopropyl betaine, 3 to 4 percent of sodium lauryl ether sulfate and 8 to 10 percent of glycerol. The addition of a small amount of garden balsam stem (herb of Chinese medicinal herbs) oil can further shorten the killing time and improve the sterilization effect, which is related to the synergistic effect brought by the permeability of the garden balsam stem (herb of Chinese medicinal herbs) and the auxiliary sterilization performance of the garden balsam stem (herb of Chinese medicinal herbs).

According to an embodiment of the present invention, the balance of the above hand sanitizer is preferably purified water.

According to a second aspect of the invention, there is provided a method of producing a hand sanitizer as described above, comprising:

mixing purified water and EDTA disodium uniformly and heating to about 85 ℃;

then adding sodium chloride, hydroxymethyl propyl cellulose, sodium methyl cocoyl taurate, cocamide DEA, cocamidopropyl betaine, sodium lauryl ether sulfate and glycerol respectively;

stirring to fully dissolve the mixture, and cooling to normal temperature; and

and finally adding the atractylodes rhizome oil, the cassia twig oil and the optional garden balsam stem oil, and uniformly stirring.

The production method according to the present invention, wherein the atractylodes rhizome oil and/or the cassia twig oil and/or the garden balsam stem oil is preferably continuously prepared by the following processes:

adding rhizoma Atractylodis rhizome powder and/or ramulus Cinnamomi powder and/or caulis et folium Gaultheriae Yunnanensis powder into an extractor equipped with a screw conveyor;

heating a first liquid storage tank filled with purified water to generate steam;

condensing the vapor into liquid water by a cooler, and introducing the liquid water into an extractor to immerse rhizoma atractylodis rhizome powder and/or cassia twig powder and/or garden balsam stem powder;

when the liquid level in the extractor is higher than the highest point of a siphon pipe connecting the extractor and the first liquid storage tank, the extracting solution in the extractor automatically enters the first liquid storage tank through the siphon pipe;

when the extract in the extractor turns colorless, starting a screw conveyor in the extractor to discharge rhizoma atractylodis rhizome wet powder and/or cassia twig wet powder and/or garden balsam stem wet powder out of the extractor, and simultaneously adding (fresh) rhizoma atractylodis rhizome powder and/or cassia twig powder and/or garden balsam stem powder into the extractor;

stopping heating the first liquid storage tank and simultaneously starting the second liquid storage tank to continue the steps; and

separating the extractive solution in the first liquid storage tank to obtain rhizoma Atractylodis oil and/or ramulus Cinnamomi oil and/or caulis et folium Gaultheriae Yunnanensis oil.

According to a preferred embodiment of the present invention, the vegetable oil extraction process is performed after mixing the rhizome powder of atractylodes rhizome and the powder of cinnamomi ramulus and optionally the powder of speranskia tuberculata in proportion. In this case, since the active plant components undergo a high-temperature reaction bonding process during extraction, a stronger synergistic bactericidal effect can be formed.

The volatile oil of rhizoma Atractylodis of the present invention can be extracted from dried rhizome of Atractylodes lancea or Atractylodes chinensis of Atractylodes of Compositae. The rhizoma Atractylodis volatile oil mainly contains volatile substances such as beta-eucalyptol, atractylodin, atractylone, atractylol, elemene oleyl alcohol, etc. Not only can kill mycobacterium tuberculosis, pseudomonas aeruginosa and the like in the air to a certain extent, but also has strong killing effect on escherichia coli and staphylococcus aureus.

The (cinnamon twig) volatile oil of the invention can be extracted from the dried tender branches of cinnamon belonging to the family lauraceae. The cassia twig volatile oil mainly contains volatile substances such as benzaldehyde, phenylpropyl aldehyde, trans-cinnamic aldehyde, cinnamic aldehyde and the like, and has remarkable antibacterial, sedative, analgesic and other effects.

The inventor finds that the main active ingredients of the atractyl and the like in the rhizoma atractylodis volatile oil and the main active ingredient of the cinnamaldehyde and the like in the cassia twig volatile oil have synergistic bactericidal effects, the atractyl and the cinnamaldehyde are combined in a specific proportion, the total dosage is reduced, and meanwhile, the atractyl and the cinnamaldehyde have obvious killing and inhibiting effects on staphylococcus aureus, streptococcus and anaerobic streptococcus.

Although the invention can also be practiced with conventional extraction techniques such as CO₂Supercritical (or subcritical) fluid extraction is used to extract plant (volatile) oils such as rhizoma Atractylodis volatile oil and/or ramulus Cinnamomi volatile oil, but the present invention preferably achieves rapid industrial scale extraction of plant oils by the following extraction system.

Thus, according to a third aspect of the present invention, there is also provided a vegetable (volatile) oil extraction system comprising:

an extractor having a charging port and a discharging port, in which a screw conveyor is provided;

the first liquid storage tank is selectively communicated with the extractor through a first gas-liquid part loop and a first siphon part loop respectively;

the second liquid storage tank is selectively communicated with the extractor through a second gas-liquid part loop and a second siphon part loop respectively;

a cooler disposed on the first and second gas-liquid portion circuits adjacent the extractor.

According to the extraction system, the first gas-liquid partial loop comprises a first vertical pipeline communicated with the top of the first liquid storage tank, a common vertical pipeline communicated with the top of the extractor, and a horizontal pipeline communicated with the first vertical pipeline and the extractor and provided with a valve; the second gas-liquid part loop comprises a second vertical pipeline communicated with the top of the second liquid storage tank, a common vertical pipeline communicated with the top of the extractor, and a horizontal pipeline communicated with the second vertical pipeline and the extractor and provided with a valve; and the first siphon part loop and the second siphon part loop are respectively provided with a valve.

According to the extraction system of the invention, the first siphon portion circuit and the second siphon portion circuit respectively have an upward bent portion and their highest point is lower than the top of the extractor.

The pure natural plant essential oil sterilization hand sanitizer can kill bacteria and eliminate bacterial plaque; the product has mild decontamination effect, can be used for skin disinfection, and can effectively inhibit infection of mold and fungus. The hand sanitizer product is non-toxic, harmless, green and environment-friendly, has no stimulation and side effect on a human body, and has a quick and lasting killing effect on common harmful bacteria such as staphylococcus aureus, candida albicans, escherichia coli, streptococcus and the like.

The invention also uses a high-efficiency (uninterrupted) vegetable oil extraction system, thereby greatly improving the production efficiency of the vegetable oil and realizing the industrialized mass production of the pure natural plant essential oil sterilization hand sanitizer. This is incomparable with conventional laboratory extraction techniques.

According to the formula of the hand sanitizer, a plurality of natural surfactants, foaming agents and emulsifiers are organically combined, so that the hand sanitizer has the excellent sterilization performance, excellent washing and decontamination capability and comfortable skin feeling after use.

Drawings

Fig. 1 is a schematic diagram of a vegetable oil extraction system (apparatus) according to the present invention.

Detailed Description

Vegetable oil extraction

The invention designs a rapid (uninterrupted) vegetable oil extraction system to meet the requirements of vegetable oil raw materials required in the industrial production of the hand sanitizer. In the present invention, the vegetable oil, the essential oil and the volatile oil are the same, and are the extracts of the corresponding plants mentioned below.

Referring to fig. 1, the vegetable oil extraction system of the present invention includes first and

second storage tanks

10 and 20, a cooler 30, and an extractor 40.

The first tank 10 has a drain pipe 11 on the bottom thereof, on which a valve 12 is mounted, the top thereof being communicated with the top of the extractor 40 through a first gas-liquid portion circuit, and the upper side thereof being communicated with the bottom of the extractor 40 through a first siphon portion circuit 50. The first gas-liquid partial circuit comprises a first vertical pipe 13 communicating with the top of the first tank 10, a common vertical pipe 33 communicating with the top of the extractor 40, and a horizontal pipe communicating the two and provided with a valve 14. The first siphon part circuit 50 is provided with a valve 51. As shown in fig. 1, the first siphon portion circuit 50 has an upwardly bent portion and its highest point is below the top of the extractor 40.

Similarly, the second tank 20 has a drain 21 on the bottom to which a valve 22 is mounted, the top communicating with the top of the extractor 40 through a second liquid-gas portion circuit, and the upper side communicating with the bottom of the extractor 40 through a second siphon portion circuit 60. The second gas-liquid portion circuit comprises a second vertical line 23 communicating with the top of the second tank 20, a common vertical line 33 communicating with the top of the extractor 40, and a horizontal line communicating the two and provided with a valve 24. The second siphon part circuit 60 is provided with a valve 61. As shown in fig. 1, first siphon portion circuit 60 also has an upwardly bent portion with its highest point below the top of extractor 40.

In fig. 1, the first gas-liquid partial circuit and the second gas-liquid partial circuit are shown with a common vertical line 33, the common vertical line 33 passing through the cooler 30. In an alternative embodiment, the first and second gas-liquid partial circuits may also not have a common vertical line 33, but rather have a vertical line through the cooler 30, in which case the

valves

14 and 24 may be omitted.

An auger 44 is mounted in the extractor 40 to convey (dried) plant particles or powder fed from the top feed port 41 of the extractor 40 along the length of the extractor (horizontally as shown) to the vicinity of the extractor discharge port 42. The powder particles in the screw conveyor 44 will be in direct contact with the liquid in the extractor 40.

The working principle of the above-described extraction system is briefly described below.

First, plant granules or powder such as rhizome granules of atractylodes lancea or cassia twig or speranskia herb granules are added through the feed port 41 of the extractor 40, and the screw conveyor 44 is started to lay the plant granules in the vicinity of the discharge port 42 of the extractor 40 in the horizontal direction of the extractor 40.

Valve 12 and valve 22 are closed and then purified water, which occupies about two thirds of the tank volume, is added to first reservoir 10 and second reservoir 20, respectively.

Closing valve 24 and valve 61;

valves

14 and 51 are opened.

The chiller 30 is started.

The first liquid storage tank 10 is heated until and the pure water therein is kept boiling continuously to form water vapor continuously.

The water vapor formed in the first storage tank 10 passes upwardly through the first vertical pipe 13 and the horizontal pipe and then downwardly through the common vertical pipe 33, and is changed into condensed water while passing through the cooler 30, and is introduced into the extractor 40.

When the liquid level in the extractor 40 exceeds the highest point of the first siphon portion circuit 50, the extracted liquid therein will be returned to the first liquid storage tank 10 through the first siphon portion circuit 50.

When the extraction liquid passing through the first siphon portion circuit 50 becomes substantially colorless, the heating of the first liquid storage tank 10 is stopped, and the auger 44 is activated to discharge the wet plant powder therein out of the extractor 40 through the discharge port 42.

Simultaneously or immediately with the above-described discharge action of the screw conveyor 44, further (fresh) plant particles are added through the feed opening 41 of the extractor 40, still through the screw conveyor 44 up to the vicinity of the discharge opening 42 of the extractor 40.

Closing valve 14 and valve 51;

valves

24 and 61 are opened.

The second reservoir 20 is heated to perform the same extraction process.

The valve 12 is opened to discharge the extract in the first reservoir tank 10 through the drain pipe 11 and then separated into distilled water and vegetable (extract) oil.

As described above, the first reservoir tank 10 and the second reservoir tank 20 are operated alternately and cooperate with the rapid transfer/discharge of the screw conveyor 44, thereby achieving the (uninterrupted) rapid industrial-scale extraction of the vegetable oil.

Preparation of hand sanitizer

Mixing purified water and EDTA disodium uniformly and heating to about 85 ℃;

stirring to fully dissolve the mixture, and cooling to normal temperature; and

and adding vegetable oil such as rhizoma Atractylodis oil and ramulus Cinnamomi oil, stirring, and packaging.

Hand sanitizer comparative examples and examples with different components and proportions

Comparative example 1

Rhizoma atractylodis volatile oil: 1.0%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerol 8%, purified water: and (4) the balance.

Comparative example 2

Rhizoma atractylodis volatile oil: 1.5%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerol 10%, purified water: and (4) the balance.

Comparative example 3

Cassia twig essential oil: 0.8%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerol 8%, purified water: and (4) the balance.

Comparative example 4

Cassia twig essential oil: 1.2%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerol 10%, purified water: and (4) the balance.

Example 1

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 0.8%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerol 8%, purified water: and (4) the balance.

Example 2

Rhizoma atractylodis volatile oil: 1.5% and cassia twig volatile oil: 1.2%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerol 10%, purified water: and (4) the balance.

Example 3

Rhizoma atractylodis volatile oil: 1.5% and cassia twig volatile oil: 0.8%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerol 8%, purified water: and (4) the balance.

Example 4

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 1.2%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerol 10%, purified water: and (4) the balance.

Example 5

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 1.0%, sodium chloride 0.15%, hydroxymethyl propyl cellulose 0.6%, disodium EDTA 0.15%, sodium methyl cocoyl taurate 6.5%, cocamide DEA 3.5%, cocamidopropyl betaine 8.5%, sodium lauryl ether sulfate 3.5%, glycerin 8.5%, purified water: and (4) the balance.

Example 6

Rhizoma atractylodis volatile oil: 1.1% and cassia twig volatile oil: 0.9%, sodium chloride 0.15%, hydroxymethyl propyl cellulose 0.6%, disodium EDTA 0.15%, sodium methyl cocoyl taurate 6.5%, cocamide DEA 3.5%, cocamidopropyl betaine 9.5%, sodium lauryl ether sulfate 3.5%, glycerin 9.5%, purified water: and (4) the balance.

Example 7

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 1.1%, sodium chloride 0.15%, hydroxymethyl propyl cellulose 0.6%, disodium EDTA 0.15%, sodium methyl cocoyl taurate 6.5%, cocamide DEA 3.5%, cocamidopropyl betaine 8.5%, sodium lauryl ether sulfate 3.5%, glycerin 8.5%, purified water: and (4) the balance.

Example 8

Rhizoma atractylodis volatile oil: 1.1% and cassia twig volatile oil: 0.8%, sodium chloride 0.15%, hydroxymethyl propyl cellulose 0.6%, disodium EDTA 0.15%, sodium methyl cocoyl taurate 6.5%, cocamide DEA 3.5%, cocamidopropyl betaine 9.5%, sodium lauryl ether sulfate 3.5%, glycerin 9.5%, purified water: and (4) the balance.

Example 9

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 0.8%, medicated leaven oil 0.1%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerin 8%, purified water: and (4) the balance.

Example 10

Rhizoma atractylodis volatile oil: 1.5% and cassia twig volatile oil: 1.2%, medicated leaven oil 0.1%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerin 10%, purified water: and (4) the balance.

Example 11

Rhizoma atractylodis volatile oil: 1.5% and cassia twig volatile oil: 0.8%, medicated leaven oil 0.3%, sodium chloride 0.1%, hydroxymethyl propyl cellulose 0.5%, disodium EDTA 0.1%, sodium methyl cocoyl taurate 6%, cocamide DEA 3%, cocamidopropyl betaine 8%, sodium lauryl ether sulfate 3% -4%, glycerin 8%, purified water: and (4) the balance.

Example 12

Rhizoma atractylodis volatile oil: 1.0% and cassia twig volatile oil: 1.2%, medicated leaven oil 0.2%, sodium chloride 0.2%, hydroxymethyl propyl cellulose 0.7%, disodium EDTA 0.2%, sodium methyl cocoyl taurate 7%, cocamide DEA 4%, cocamidopropyl betaine 10%, sodium lauryl ether sulfate 4%, glycerin 10%, purified water: and (4) the balance.

Example 13

The other steps are the same as example 5, except that the rhizoma atractylodis volatile oil and the cassia twig volatile oil are prepared by mixing rhizoma atractylodis rhizome powder and cassia twig powder in proportion and then extracting.

Example 14

The other steps are the same as example 11, except that the atractylodes rhizome volatile oil, the cassia twig volatile oil and the medicinal herb leaven oil are prepared by mixing the atractylodes rhizome powder, the cassia twig powder and the medicinal herb leaven powder in proportion and then extracting.

Quantitative sterilization test of suspension

(1) Three strains of staphylococcus aureus, streptococcus and anaerobic streptococcus are selected to respectively prepare the bacterial suspension for experiments according to the disinfection technical specification, and the concentration is 1 x 108cfu/ml to 5 x 108 cfu/ml.

(2) Taking a large sterile test tube for a disinfection test, firstly adding 0.5ml of test bacterial suspension, then adding 0.5ml of organic interference substance, uniformly mixing, placing in a water bath at 20 +/-1 ℃ for 5min, sucking 4.0ml of the hand sanitizer prepared in each example and comparative example by using a sterile pipette, injecting into the hand sanitizer, rapidly mixing uniformly and immediately timing.

(3) And (3) respectively sucking 0.5ml of mixed solution of the test bacteria and the hand sanitizer into 4.5ml of sterilized neutralizer after the test bacteria and the hand sanitizer interact for each preset time, and uniformly mixing.

(4) Adding neutralizer into the mixed solution of test bacteria and hand sanitizer for 10min, sucking 1.0ml of sample solution, measuring the number of viable bacteria by viable bacteria culture counting method, and inoculating 2 plates to each tube of sample solution. If the number of colonies growing on the plate is large, serial 10-fold dilution can be performed, and viable bacteria culture counting can be performed.

(5) Meanwhile, the diluted solution is used for replacing the hand sanitizer, and a parallel test is carried out to be used as a positive control.

(6) Culturing all test samples in an incubator at 37 ℃, and culturing the bacterial propagules for 48 hours to observe the final result; the bacterial spores were cultured for 72h to observe the final result.

(7) The test was repeated 3 times, and the viable bacteria concentration (cfu/ml) of each group was calculated and converted to a logarithmic value (N), and then the killing logarithmic value was calculated as follows:

log Kill (KL) log of mean viable bacteria concentration of control group (No) -log of viable bacteria concentration of test group (Nx)

The test results are shown in table 1.

Table 1: quantitative sterilization test result of suspension

The results of the sterilization tests show that:

the high-concentration atractylodes oil and cassia twig oil have strong killing effect on staphylococcus aureus when being used independently, the killing log-rank (KL) can even reach 5.14, but the killing log-rank (KL) on streptococcus and anaerobic streptococcus is poor (both are lower than 5.0). The screening results of the examples 1 to 8 show that the combination of the two has a synergistic effect on the main active ingredients in the rhizoma atractylodis volatile oil and the cassia twig volatile oil, and the dosage of the rhizoma atractylodis volatile oil and the cassia twig volatile oil is reduced, and the rhizoma atractylodis volatile oil and the cassia twig volatile oil can obviously kill staphylococcus aureus, streptococcus and anaerobic streptococcus. For example, in the quantitative bactericidal test of the suspensions in comparative examples 1 to 4, the comprehensive average killing logarithm values of three bacteria of the rhizoma atractylodis volatile oil hand sanitizer with the concentrations of 1.0% and 1.5% are respectively 3.93 and 4.41, and the comprehensive average killing logarithm values of three bacteria of the cassia twig volatile oil hand sanitizer with the concentrations of 0.8% and 1.2% are respectively 4.13 and 4.45; when the two are mixed for use, the comprehensive average killing logarithm value of the three bacteria is more than 5.0 no matter how the concentration is adjusted, and the fact that the atractylodes rhizome oil and the cassia twig oil have the synergistic bactericidal effect is fully shown. Examples 9-12 show that the addition of a small amount of herbal oil further synergistically enhanced the bactericidal effect.

In addition, 20 men and women with normal physical conditions and ages of 18-60 are selected to be subjected to skin irritation test, the testers use the liquid soap of example 10 to wash hands before meals for 3 times every day for 5 minutes, other chemical products are not contacted during the test period, no additional hand skin care is carried out, and statistics of the results after one week, two weeks and one month show that: on the premise of no skin care and maintenance, no matter men or women and people at all ages, the hand sanitizer has no side effect on human bodies, namely, the skin has no phenomena of irritation, itching and redness, and the skin feels comfortable and refreshing after use.

Claims

1. A hand sanitizer comprising: 1.0 to 1.5 percent of rhizoma atractylodis volatile oil, 0.8 to 1.2 percent of cassia twig volatile oil, 0.1 to 0.2 percent of sodium chloride, 0.5 to 0.7 percent of hydroxymethyl propyl cellulose, 0.1 to 0.2 percent of disodium EDTA, 6 to 7 percent of methyl cocoyl taurate, 3 to 4 percent of cocamide DEA, 8 to 10 percent of cocamidopropyl betaine, 3 to 4 percent of sodium lauryl ether sulfate and 8 to 10 percent of glycerol.

2. The hand sanitizer of claim 1 comprising: 1.0 to 1.2 percent of rhizoma atractylodis volatile oil, 0.8 to 1.0 percent of cassia twig volatile oil, 0.1 to 0.2 percent of sodium chloride, 0.5 to 0.7 percent of hydroxymethyl propyl cellulose, 0.1 to 0.2 percent of disodium EDTA, 6 to 7 percent of methyl cocoyl taurate, 3 to 4 percent of cocamide DEA, 8 to 10 percent of cocamidopropyl betaine, 3 to 4 percent of sodium lauryl ether sulfate and 8 to 10 percent of glycerol.

3. The hand sanitizer of claim 1 comprising: rhizoma atractylodis volatile oil: 1.0-1.5% of cassia twig volatile oil: 0.8% -1.2%, garden balsam stem oil: 0.1 to 0.3 percent of sodium chloride, 0.1 to 0.2 percent of sodium chloride, 0.5 to 0.7 percent of hydroxymethyl propyl cellulose, 0.1 to 0.2 percent of EDTA disodium, 6 to 7 percent of methyl cocoyl sodium taurate, 3 to 4 percent of cocamide DEA, 8 to 10 percent of cocamidopropyl betaine, 3 to 4 percent of sodium lauryl ether sulfate and 8 to 10 percent of glycerol.

4. A hand sanitizer according to any one of claims 1 to 3, wherein the balance is purified water.

5. A method of producing the hand sanitizer of claim 1 comprising:

mixing purified water and EDTA disodium uniformly and heating to about 85 ℃;

stirring to fully dissolve the mixture, and cooling to normal temperature; and

finally adding the atractylodes oil and the cassia twig oil, and uniformly stirring.

6. The production method according to claim 5, wherein the atractylodes rhizome oil and/or the cassia twig oil is continuously prepared by the following processes:

adding rhizoma Atractylodis rhizome powder and/or ramulus Cinnamomi powder into an extractor equipped with a screw conveyor;

condensing the vapor into liquid water by a cooler, and introducing the liquid water into an extractor to immerse rhizoma atractylodis rhizome powder and/or cassia twig powder;

when the extracting solution in the extractor is colorless, starting a screw conveyor in the extractor to discharge rhizoma atractylodis rhizome wet powder and/or cassia twig wet powder out of the extractor, and simultaneously adding rhizoma atractylodis rhizome powder and/or cassia twig powder into the extractor;

separating the extractive solution in the first liquid storage tank to obtain rhizoma Atractylodis oil and/or ramulus Cinnamomi oil.