CN105557590B - A kind of Australia freshwater lobster in-vitro hatching method - Google Patents
A kind of Australia freshwater lobster in-vitro hatching method Download PDFInfo
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- CN105557590B CN105557590B CN201610028938.3A CN201610028938A CN105557590B CN 105557590 B CN105557590 B CN 105557590B CN 201610028938 A CN201610028938 A CN 201610028938A CN 105557590 B CN105557590 B CN 105557590B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to lobsters to cultivate field, discloses a kind of Australia freshwater lobster in-vitro hatching method.After obtaining fertilized eggs, first fertilized eggs are cleaned, after be put into in-vitro hatching disk and carry out artificial incubation.Prefilter bucket, ozonizer, heat-generating pipe and the aerator and separation net that in-vitro hatching disk is equipped with and water body matches, fertilized eggs are placed on separation net, in-vitro hatching pan bottom sets air stone manufacture water flow, fertilized eggs are enabled to keep pico- activity, ozone machine time system controls periodically disinfection, and fertilized eggs can hatch young shrimp by hatching in about 40-50 days.Using in-vitro hatching method of the present invention, the infection rate of fertilized eggs can be down to 10% hereinafter, the survival rate of hatching substantially increases the survival rate of incubating oosperm, can effectively improve shrimp seedling yield up to 90% or more, have significant economic benefit.
Description
Technical field
The present invention relates to lobsters to cultivate field, particularly relates to a kind of Australia freshwater lobster in-vitro hatching method.
Background technique
Australia freshwater lobster (Cherax quadricarinatus), scientific name are four ridge bare hull south huge legendary turtle shrimps also known as red chela chela
Shrimp originates in Australia, exactly likes marine lobster, is most rare one of economic freshwater shrimp species in the world at present.The shrimp body
Color breen, the outer tip end of the chela of mature hero shrimp has the scarlet band of a film quality, beautiful good-looking, therefore is known as red claw crayfish again.Australia
Freshwater lobster mates in vitro, lays eggs, and hatches shrimp seedling by female shrimp oogenesis, and can independently become young shrimp in parent, works as shrimp seedling
When being detached from parent, individual lengths can reach 0.8 centimetre, can directly launch pond culture.
And Australia freshwater lobster egg load is few, and can move about during oogenesis, and fertilized eggs is easily caused to fall or touch
Damage is bad, seriously affects the survival rate of incubating oosperm, it is difficult to ensure has enough seedling amounts to supply, seriously restricts Australia fresh water dragon
The development of shrimp aquaculture.Meanwhile once female shrimp is dead during oogenesis hatches, the intact fertilized eggs left are difficult to smoothly send out
It educates, causes the fertilized eggs of a large amount of health to be difficult to develop into young shrimp, cause largely to waste.
Summary of the invention
The invention proposes a kind of Australia freshwater lobster in-vitro hatching methods, fertilized eggs can be carried out in-vitro hatching, from
And various limitations of female shrimp oogenesis hatching are got rid of, the fertilized eggs that dead female shrimp is left can also be recycled, can effectively be mentioned
The survival rate of high incubating oosperm increases young shrimp yield.
The technical scheme of the present invention is realized as follows:
A kind of Australia freshwater lobster in-vitro hatching method, which comprises the following steps:
(1) in female shrimp after fertilization, female shrimp is caught during labor and is individually positioned on obstetric table, adjustment water temperature is 29-30 DEG C,
Temperature should promote 0.4-0.6 DEG C daily, promoted and arrive 34-36 DEG C；Collect the fertilized eggs that female shrimp gives birth to, cleaning；
(2) fertilized eggs after cleaning are put into in-vitro hatching disk and carry out in-vitro hatching, until hatching young shrimp；Wherein, in vitro
Prefilter bucket, ozonizer, heat-generating pipe and the aerator that incubation plate is equipped with and water body matches；In in-vitro hatching disk setting every
Off-network, fertilized eggs are placed on separation net, and in-vitro hatching pan bottom sets air stone manufacture water flow, and fertilized eggs is enabled to keep pico- activity,
It is periodically sterilized with ozone machine, periodically bad egg white is managed away.
Further, the fertilized eggs in the step (1) are that female shrimp gives birth to the still fertilized eggs of non-oogenesis and/or oogenesis hatching
The fertilized eggs that dead female shrimp leaves in the process.
Further, the method that the cleaning of fertilized eggs uses in the step (1) are as follows: with mass fraction be 2-4% salt water logging
Bubble 3-6 minutes.
Further, step (2) water temperature controls 27-29 DEG C.
Further, the separation net being arranged in step (2) the in-vitro hatching disk is 60-70 mesh, 4-6 lis of separation net separation disc bottom
Rice.
Further, water depth is 15-20 centimetres in step (2) the in-vitro hatching disk.
Further, in the step (2), water velocity is the 1/3-2/3 of water inlet or discharge outlet water speed.
Further, in the step (2), disinfection in ozone machine every 0.5-1.5 days is primary, and each disinfecting time is 30-50 points
Clock；The frequency for clearing up bad ovum is that every 1-2 days cleaning is primary.
Further, in the step (2), the time of the in-vitro hatching is 40-50 days.
Beneficial effects of the present invention:
In-vitro hatching method of the present invention be avoided that female shrimp embrace walk about when incubating cause fertilized eggs to fall, breakage etc. is asked
Topic, considerably increases the survival rate of fertilized eggs.Meanwhile during incubating oosperm, manufacture water flow keeps the pico- of fertilized eggs
Movement simulates the natural incubation condition of fertilized eggs, and periodically carries out disinfection in conjunction with ozonizer, can effectively prevent fertilized eggs
The bacteriums such as water mold are infected, the survival rate of hatching is improved.Using in-vitro hatching method of the present invention, the infection rate of fertilized eggs
10% can be down to hereinafter, the survival rate of hatching substantially increases the survival rate of incubating oosperm, shrimp seedling yield up to 90% or more
It greatly increases, there is significant economic benefit.
In-vitro hatching method of the present invention can be used for the fertilized eggs fallen when hatching female shrimp oogenesis and dead mother
The fertilized eggs that shrimp is left increase shrimp seedling yield to reduce the waste of fertilized eggs.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical solution in the present invention is clearly and completely described, it is clear that
Described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, every other embodiment obtained by those of ordinary skill in the art without making creative efforts belong to
The scope of protection of the invention.
Embodiment 1
A kind of Australia freshwater lobster in-vitro hatching method, comprising the following steps:
(1) in female shrimp after fertilization, development of fertilized ova degree is concluded according to the animation of female shrimp, catches female shrimp point during labor
It is not placed on obstetric table, adjustment water temperature is 29 DEG C, and temperature should promote 0.4 DEG C daily, is when laying eggs when being promoted to 34 DEG C
Between；Collect the fertilized eggs that female shrimp gives birth to, be that 2% salt water is impregnated 3 minutes and cleaned with mass fraction, can be put into after cleaning from
Body incubation plate carries out in-vitro hatching；
(2) fertilized eggs after cleaning are put into in-vitro hatching disk and carry out in-vitro hatching, until paying young shrimp；Wherein incubate in vitro
Change prefilter bucket, ozonizer, heat-generating pipe and the aerator that disk is equipped with and water body matches；Prefilter bucket can be to incubating in vitro
The water body changed in disk is filtered, sterilizes, and keeps the stabilization of water quality in in-vitro hatching disk, and aerator provides for the development of fertilized eggs
Sufficient oxygen；Water depth is 15 centimetres in in-vitro hatching disk, is heated using heat-generating pipe to water body, controls water temperature 27
℃；The separation net of 60 mesh of setting, fertilized eggs are placed on separation net in in-vitro hatching disk, and separation net tries to get to the heart of a matter 4 apart from in-vitro hatching disk
Centimetre, in-vitro hatching pan bottom sets air stone manufacture water flow, and water velocity is in-vitro hatching disk water inlet or discharge outlet water speed
1/3, enable fertilized eggs keep pico- activity, primary with disinfection in ozone machine every 0.5 day, each disinfecting time is 30 minutes, daily
Primary bad ovum is cleared up, fertilized eggs can hatch young shrimp by hatching in 40 days.
Embodiment 2
A kind of Australia freshwater lobster in-vitro hatching method, comprising the following steps:
(1) in female shrimp after fertilization, development of fertilized ova degree is concluded according to the animation of female shrimp, catches female shrimp point during labor
It is not placed on obstetric table, adjustment water temperature is 30 DEG C, and temperature should promote 0.6 DEG C daily, is when laying eggs when being promoted to 36 DEG C
Between；Collect female shrimp give birth to but the fertilized eggs that leave of female shrimp dead during the fertilized eggs of non-oogenesis and oogenesis, use quality
Score is that the immersion of 4% salt water is cleaned for 6 minutes, and in-vitro hatching disk can be put into after cleaning and carries out in-vitro hatching；
(2) prefilter bucket, ozonizer, heat-generating pipe and the aerator that in-vitro hatching disk is equipped with and water body matches；Before
The water body in in-vitro hatching disk can be filtered, sterilize by setting filter vat, keep the stabilization of water quality in in-vitro hatching disk, aerator
Sufficient oxygen is provided for the development of fertilized eggs；Water body is 20 centimetres deep in in-vitro hatching disk, is heated using heat-generating pipe to water body, control
29 DEG C of water temperature processed；The separation net of 70 mesh of setting, fertilized eggs are placed on separation net in in-vitro hatching disk, and separation net distance is in vitro
Incubation plate is tried to get to the heart of a matter 6 centimetres, in-vitro hatching pan bottom set air stone manufacture water flow, water velocity be in-vitro hatching disk water inlet or
The 2/3 of discharge outlet water speed enables fertilized eggs keep pico- activity, primary with disinfection in ozone machine every 2 days, and each disinfecting time is 50 points
Clock, clears up primary bad ovum for every 2 days, and fertilized eggs can hatch young shrimp by hatching in 50 days.
Embodiment 3
A kind of Australia freshwater lobster in-vitro hatching method, comprising the following steps:
(1) in female shrimp after fertilization, development of fertilized ova degree is concluded according to the animation of female shrimp, catches female shrimp point during labor
It is not placed on obstetric table, adjustment water temperature is 28 DEG C, and temperature should promote 0.5 DEG C daily, is when laying eggs when being promoted to 35 DEG C
Between；The fertilized eggs that dead female shrimp leaves during collection oogenesis are that the immersion of 3% salt water is cleaned for 5 minutes with mass fraction,
It can be put into in-vitro hatching disk after cleaning and carry out in-vitro hatching；
(2) prefilter bucket, ozonizer, heat-generating pipe and the aerator that in-vitro hatching disk is equipped with and water body matches；Before
The water body in in-vitro hatching disk can be filtered, sterilize by setting filter vat, keep the stabilization of water quality in in-vitro hatching disk, aerator
Sufficient oxygen is provided for the development of fertilized eggs；Water body is 18 centimetres deep in in-vitro hatching disk, is heated using heat-generating pipe to water body, control
28 DEG C of water temperature processed；The separation net of 65 mesh of setting, fertilized eggs are placed on separation net in in-vitro hatching disk, and separation net distance is in vitro
Incubation plate is tried to get to the heart of a matter 5 centimetres, in-vitro hatching pan bottom set air stone manufacture water flow, water velocity be in-vitro hatching disk water inlet or
The 1/2 of discharge outlet water speed enables fertilized eggs keep pico- activity, primary with disinfection in ozone machine every 1 day, and each disinfecting time is 40 points
Clock daily manages away bad egg white, and fertilized eggs can hatch young shrimp by hatching in 45 days.
Embodiment 4
A kind of Australia freshwater lobster in-vitro hatching method, comprising the following steps:
(1) in female shrimp after fertilization, development of fertilized ova degree is concluded according to the animation of female shrimp, catches female shrimp point during labor
It is not placed on obstetric table, adjustment water temperature is 30 DEG C, and temperature should promote 0.5 DEG C daily, is when laying eggs when being promoted to 35 DEG C
Between；Collect female shrimp give birth to but the fertilized eggs that leave of female shrimp dead during the fertilized eggs that do not embrace and oogenesis, with quality point
Number is that the immersion of 3% salt water is cleaned for 5 minutes, and in-vitro hatching disk can be put into after cleaning and carries out in-vitro hatching；
(2) prefilter bucket, ozonizer, heat-generating pipe and the aerator that in-vitro hatching disk is equipped with and water body matches；Before
Water body in in-vitro hatching disk can be filtered, sterilize by setting filter vat, keep the stabilization of water quality in in-vitro hatching disk, and aerator is
The development of fertilized eggs provides sufficient oxygen；Water body is 20 centimetres deep in in-vitro hatching disk, is heated using heat-generating pipe to water body, control
28 DEG C of water temperature；The separation net of 60 mesh of setting, fertilized eggs are placed on separation net in in-vitro hatching disk, and separation net distance is incubated in vitro
Change disk to try to get to the heart of a matter 5 centimetres, in-vitro hatching pan bottom sets air stone manufacture water flow, and water velocity is in-vitro hatching disk water inlet or row
The 1/2 of mouth of a river water speed enables fertilized eggs keep pico- activity, primary with disinfection in ozone machine every 1 day, and each disinfecting time is 40 points
Clock daily manages away bad egg white, and fertilized eggs can hatch young shrimp by hatching in 43 days.
In-vitro hatching method hatching effect of the present invention is investigated
Randomly choose 140 fertilizations will just before giving birth female shrimp, be allocated as 7 groups, every group 20, experimental group in embodiment 1-4 according to mentioning
The in-vitro hatching method of confession is hatched；Control group 1 is hatched naturally by female shrimp oogenesis, and control group 2 is hatched naturally by female shrimp oogenesis
And ozone machine is combined to sterilize, control group 3 carries out in-vitro hatching according to the method for embodiment 4, but sterilizes without ozone machine.To
After hatching young shrimp, the survival rate and infection conditions of hatching are counted respectively.The results are shown in Table 1 for it.
The in-vitro hatching method hatching effect of the present invention of table 1 is investigated
As can be seen from Table 1, the in-vitro hatching method that 4 embodiments of the invention provide, survival rate and infection rate are obvious
Higher than 3 control groups.Control group 2 is hatched using female shrimp oogenesis and ozone machine is combined to sterilize, and the infection rate of fertilized eggs is substantially reduced,
Survival rate increases, but is still below 4 embodiments, illustrates the method using ozonization, fertilized eggs can be effectively reduced
Infection rate, but due to the presence of various unfavorable factors in female shrimp oogenesis hatching process, for example, fertilized eggs are fallen, female shrimp is dead
It dies, fertilized eggs have been seriously affected the raising of fertilized eggs survival rate by rupture etc. is hit during travelling.Control group 3 using from
The method of body hatching, but due to not having to carry out ozone machine disinfection, the germs such as fertilized eggs infection water mold are high-incidence, and infection rate is obvious
Higher than 4 embodiment processing groups and other two control group, the survival rate of fertilized eggs is not only below 4 embodiment groups, relatively
In other 2 control groups, also there is different degrees of reduction.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (2)
1. a kind of Australia freshwater lobster in-vitro hatching method, which comprises the following steps:
(1) in female shrimp after fertilization, female shrimp being caught during labor and is individually positioned on obstetric table, adjustment water temperature is 29-30 DEG C, temperature
0.4-0.6 DEG C should be promoted daily, promoted and arrived 34-36 DEG C；The fertilized eggs that female shrimp gives birth to are collected, are 2-4% salt water with mass fraction
It impregnates 3-6 minutes and is cleaned；The fertilized eggs are the fertilized eggs and/or oogenesis hatching process that female shrimp gives birth to still non-oogenesis
The fertilized eggs that female shrimp of middle death leaves；
(2) fertilized eggs after cleaning are put into in-vitro hatching disk and carry out in-vitro hatching, until hatching young shrimp；Wherein, in-vitro hatching
Prefilter bucket, ozonizer, heat-generating pipe and the aerator that disk is equipped with and water body matches；Setting isolation in in-vitro hatching disk
Net, fertilized eggs are placed on separation net, and in-vitro hatching pan bottom sets air stone manufacture water flow, and water velocity is water inlet or draining
The 1/3-2/3 of saliva speed enables fertilized eggs keep pico- activity, primary with disinfection in ozonizer every 0.5-1.5 days, sterilizes every time
Time is 30-50 minutes；Periodically bad egg white is managed away, the frequency for clearing up bad ovum is that every 1-2 days cleaning is primary；Water temperature control
27-29 DEG C processed, 15-20 centimetres of water depth in in-vitro hatching disk, brooding time is 40-50 days.
2. a kind of Australia freshwater lobster in-vitro hatching method as described in claim 1, it is characterised in that: the step (2) from
The separation net being arranged in body incubation plate is 60-70 mesh, 4-6 centimetres of separation net separation disc bottom.
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| CN107568122A (en) * | 2017-09-21 | 2018-01-12 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambius clarkii embryonated egg in-vitro hatching method |
| CN110326568A (en) * | 2019-08-15 | 2019-10-15 | 中国水产科学研究院淡水渔业研究中心 | A kind of Procambius clarkii early stage shrimp ovum in-vitro breeding method |
| CN112544511B (en) * | 2020-12-09 | 2024-06-25 | 中国科学院水生生物研究所 | Device and method for in-vitro hatching of fertilized eggs of crayfish |
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| CN101720684B (en) * | 2008-11-03 | 2012-02-08 | 中国水产科学研究院淡水渔业研究中心 | Method for in vitro incubation of macrobrachium nipponense |
| CN102217567A (en) * | 2011-06-01 | 2011-10-19 | 中国水产科学研究院淡水渔业研究中心 | In-vitro culture technology for fertilized ova of beautiful white shrimps |
| CN103004656A (en) * | 2012-12-17 | 2013-04-03 | 南充市营渔水产科技有限公司 | Intensified artificial breeding method for Australian crayfishes |
| CN103314908A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Isolated breeding technique for cherax quadricarinatus eggs |
| CN104823883A (en) * | 2015-04-07 | 2015-08-12 | 高邮市阳光特种水产专业合作社 | Macrobranchium rosenbergii larvae breeding method |
| CN204811523U (en) * | 2015-07-31 | 2015-12-02 | 淮海工学院 | White shrimp embryo separation hatching apparatus of spine end |
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