CN105523810A - Seaweed azotobacter fertilizer preparation method - Google Patents
Seaweed azotobacter fertilizer preparation method Download PDFInfo
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- CN105523810A CN105523810A CN201511031121.3A CN201511031121A CN105523810A CN 105523810 A CN105523810 A CN 105523810A CN 201511031121 A CN201511031121 A CN 201511031121A CN 105523810 A CN105523810 A CN 105523810A
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- CN
- China
- Prior art keywords
- fertilizer
- azotobacter
- vinelandii
- marine alga
- seaweed
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The present invention relates to the field of preparation of fertilizers, and discloses a seaweed azotobacter fertilizer preparation method, fresh seaweed is chopped, and mixed into a nutritional supplement for even mixing to prepare a solid fermentation matrix, an azotobacter seed liquid is inoculated for solid fermentation, low-temperature drying and crushing to prepare a seaweed azotobacter fertilizer. By seaweed solid fermentation technique, azotobacter and a seaweed fertilizer are organically combined, active ingredients of the seaweed can be converted by the azotobacter, better plant growth-promoting effect is played, meanwhile the azotobacter in the seaweed azotobacter fertilizer can supplement nitrogen for plant roots by nitrogen fixation effect, during growth and reproduction process of the azotobacter, a large number of extracellular polysaccharides are secreted, and the extracellular polysaccharides are an adhesive for formation of a soil aggregate structure and maintaining of the stability of the aggregates, the soil compaction phenomenon caused by the long-term excessive application of inorganic fertilizers can be overcome, and long-term sustainable development of ecological agriculture can be promoted.
Description
Technical field
The present invention relates to fertilizer preparation field, particularly a kind of preparation method of marine alga vinelandii fertilizer.
Background technology
At present, agricultural products in China is polluted mainly agricultural chemicals, chemical fertilizer and is caused.20 beginnings of the century brought into use chemical fertilizer, and for increasing the output of agricultural-food, the supply of underwriter's based food has played vital role.But problem is also more and more many, a large amount of chemical fertilizer that uses causes soil compaction and fertility to decline, and agricultural product quality is declined, more seriously pollute agricultural-food and environment, make nitrate in soil, nitrite content exceeds standard, and and then cause body eutrophication, the deterioration of the ecological environment.Address these problems, realize agricultural-food No-harmful apple orchard, the required non-harmful new-type fertilizer of Application and Development.Recent two decades comes, and seaweed fertilizer in the world Application and Development is noticeable.Seaweed fertilizer is the forth generation fertilizer after farm organic fertilizer, chemical fertilizer, bio-fertilizer, and it has farm manure provides comprehensive nutritive ingredient, free from environmental pollution, can also culture fertility improvement soil effect; There is the quick-acting of chemical fertilizer simultaneously, there is bio-feritlizer and promote crop growth, improve output, the effect such as oil recovery enhancement and activating soil; In addition, can also expelling parasite, diseases prevention, cure the disease, improve crop drought resistance, the effect such as cold-resistant.Seaweed fertilizer is many national large scale applications in the world now, achieve very significant economic benefit, social benefit and ecological benefits.Utilize marine alga to combine with vinelandii, carry out making and the research aspect of marine alga vinelandii fertilizer, have not yet to see report.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of marine alga vinelandii fertilizer, it is by marine alga solid-fermented technique, vinelandii and marine alga fertilizer are combined, can by the conversion of the effective constituent in marine alga by vinelandii, play better Plant growth-promoting effect effect, vinelandii simultaneously in marine alga vinelandii fertilizer pass through nitrogen fixation, for root system of plant supplements nitrogen, a large amount of exocellular polysaccharides that vinelandii secrete in growth and breeding process, it is the tackiness agent forming soil aggregate and keep granule stability, can overcome because of the excessive soil compaction phenomenon used mineral manure and cause for a long time, thus promote that agroecological long-term sustainable develops.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the invention discloses a kind of preparation method of marine alga vinelandii fertilizer, preparation method is by after fresh marine alga chopping, be mixed into nutritious supplementary to mix, make solid fermentation matrix, Azotobacter seed liquor carries out solid fermentation, cryodrying, makes marine alga vinelandii fertilizer after pulverizing.
Wherein, marine alga is sea-tangle, Enteromorpha, wakame, and vinelandii are azotobacter chroococcum.
Wherein, nutritious supplementary is made up of flour, magnesium, calcium, and their weight percent respectively is 0.1-5.0%, 0.001-0.1%, 0.05-1% according to fresh seaweed weight.
Wherein, solid fermentation matrix is made up of marine alga and nutritious supplementary, after both mix according to the above ratio, and boiling 5-15 minute under the environment of 100 DEG C.
Wherein, solid fermentation process is: will be cultured to the vinelandii seed liquor of logarithmic phase, be seeded in above-mentioned solid fermentation matrix according to the ratio of weight percent 1-20%, keep solid fermentation matrix thickness within the scope of 20cm, the moisturizing of plastic covering film also prevents living contaminants, at 20-35 DEG C of temperature controlled fermentation 3-10 days.
The present invention has following beneficial effect:
1. in the present invention, the effective constituent of marine alga can not run off in solid fermentation, and particularly contained plant hormone can retain preferably; Sargassum polysaccharides can produce degraded to a certain degree after vinelandii fermentation, contributes to absorption and the growth promoting function of crop; Vinelandii in fertilizer can pass through nitrogen fixation, for root system of plant supplements nitrogen, simultaneously a large amount of exocellular polysaccharides of secreting in growth and breeding process of vinelandii, be the tackiness agent forming soil aggregate and keep granule stability, can overcome because of the excessive soil compaction phenomenon used mineral manure and cause for a long time.
2. the granulated fertilizer that the present invention obtains can ensure the activity of vinelandii effectively, extends preservation period, also improves effect, fertiliser production is with low cost, fermentation condition and equipment requirements low, technical process is easy and simple to handle, invests little, is applicable to the small-scale production of plantation producer and fertilizer source mill.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.
Embodiment 1
The invention discloses a kind of preparation method of marine alga vinelandii fertilizer, concrete steps are as follows:
Fresh for 1kg commercially available sea-tangle tap water is rinsed well repeatedly, after drying, is cut into the fragment of 1cm size; Separately get flour 4g, magnesium sulfate 0.1g, calcium carbonate 2g is dissolved in a small amount of tap water and mixes, make nutritious supplementary; Sea-tangle fragment is fully mixed with nutritious supplementary and makes solid fermentation matrix, 100 DEG C of boilings 15 minutes, cooling.
Preparation vinelandii seed liquor: primary inclined plane is cultivated and adopted Ah Xu shellfish substratum, this substratum forms: potassium primary phosphate 0.2g, magnesium sulfate 0.2g, sodium-chlor 0.2g, calcium carbonate 5g, glucose 10g, calcium sulfate 0.1g, agar 18g, water 1L, pH is 7.0, after cultivating 48 hours in 28 DEG C, inoculation secondary Ah Xu shellfish liquid nutrient medium, 36 hours can to growing logarithmic phase in 28 DEG C of shaking culture.
By the vinelandii seed liquor being cultured to logarithmic phase according to 6% ratio be seeded in above-mentioned solid fermentation matrix, after stirring, be laid in pallet by the thickness of 8cm, tight with double-deck polypropylene film capping, in 28 DEG C of solid fermentations 5 days, every day turns 3-4 time with aseptic shovel, and culture is mixed.
After having fermented, solid fermentation thing, after 50 DEG C of cryodryings, is crushed to 80 orders, makes marine alga vinelandii fertilizer.Get a certain amount of marine alga vinelandii fertilizer physiological saline to suspend, fully vibrate, by vinelandii plate count, vinelandii quantity can reach 60-100 hundred million/gram of dry weights, and culture is without unhappy smell.
Embodiment 2
Experiment purpose and method: in order to evaluate validity of the present invention and feasibility, the marine alga vinelandii fertilizer that the present embodiment prepares with embodiment 1 is for experimental subjects, iron wheat No. 1 wheat breed is grown impact research, blank group, common vinelandii group and marine alga vinelandii group are set, blank group will not be applied fertilizer, common vinelandii group bestows common vinelandii fertilizer, and marine alga vinelandii group bestows marine alga vinelandii fertilizer, and concrete operation method is as follows:
Adopt iron wheat No. 1 wheat breed, wheat seed is put into clear water and soaks 24h, use 0.1%mg/LHgCl
2solution disinfection 5min, after using distilled water flushing 3 times again, choosing wheat seed of uniform size is seeded in the soil incubation box of sterilization, common vinelandii fertilizer, marine alga vinelandii fertilizer is added respectively according to the amount of every mu of 1kg, the same operation of blank group but not fertilise, be put into illumination box to cultivate, every day waters at regular time and quantity, within the 12nd day after emerging, measures every physical signs.
Experimental result: long more blank group of the average seedling of common vinelandii group increases 5-8%, and root is long increases 2-6%; Long more blank group of the average seedling of marine alga vinelandii group increases 17-30%, and root is long increases 8-12%, and the marine alga vinelandii fertilizer prepared by method of the present invention has good Plant growth-promoting effect effect.
The above; be only the present invention's preferably embodiment, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.
Claims (5)
1. the preparation method of a marine alga vinelandii fertilizer, it is characterized in that: described preparation method is by after fresh marine alga chopping, be mixed into nutritious supplementary to mix, make solid fermentation matrix, Azotobacter seed liquor carries out solid fermentation, cryodrying, makes marine alga vinelandii fertilizer after pulverizing.
2. the preparation method of marine alga vinelandii fertilizer as claimed in claim 1, it is characterized in that: described marine alga is sea-tangle, Enteromorpha, wakame, described vinelandii are azotobacter chroococcum.
3. the preparation method of marine alga vinelandii fertilizer as claimed in claim 1 or 2, it is characterized in that: described nutritious supplementary is made up of flour, magnesium, calcium, their weight percent respectively is 0.1-5.0%, 0.001-0.1%, 0.05-1% according to fresh seaweed weight.
4. the preparation method of marine alga vinelandii fertilizer as claimed in claim 1, is characterized in that: described solid fermentation matrix is made up of marine alga and nutritious supplementary, after both mix according to the above ratio, and boiling 5-15 minute under the environment of 100 DEG C.
5. the preparation method of marine alga vinelandii fertilizer as claimed in claim 1, it is characterized in that: described solid fermentation process is: will the vinelandii seed liquor of logarithmic phase be cultured to, be seeded in above-mentioned solid fermentation matrix according to the ratio of weight percent 1-20%, keep solid fermentation matrix thickness within the scope of 20cm, the moisturizing of plastic covering film also prevents living contaminants, at 20-35 DEG C of temperature controlled fermentation 3-10 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511031121.3A CN105523810A (en) | 2015-12-31 | 2015-12-31 | Seaweed azotobacter fertilizer preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511031121.3A CN105523810A (en) | 2015-12-31 | 2015-12-31 | Seaweed azotobacter fertilizer preparation method |
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| CN105523810A true CN105523810A (en) | 2016-04-27 |
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| CN201511031121.3A Pending CN105523810A (en) | 2015-12-31 | 2015-12-31 | Seaweed azotobacter fertilizer preparation method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104496577A (en) * | 2014-12-25 | 2015-04-08 | 刘长生 | Composite microbial fertilizer and preparation method thereof |
| CN104817398A (en) * | 2015-04-28 | 2015-08-05 | 众德肥料（烟台）有限公司 | Basic-protein bio-organic fertilizer production technology |
| CN104926546A (en) * | 2015-07-16 | 2015-09-23 | 句容市容北茶文化有限公司 | Organic fertilizer for apocarya seedlings and preparation method of organic fertilizer |
| CN105085111A (en) * | 2015-08-12 | 2015-11-25 | 生之道生态农业科技股份有限公司 | Biological-organic fertilizer special for corn and preparation method thereof |
-
2015
- 2015-12-31 CN CN201511031121.3A patent/CN105523810A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104496577A (en) * | 2014-12-25 | 2015-04-08 | 刘长生 | Composite microbial fertilizer and preparation method thereof |
| CN104817398A (en) * | 2015-04-28 | 2015-08-05 | 众德肥料（烟台）有限公司 | Basic-protein bio-organic fertilizer production technology |
| CN104926546A (en) * | 2015-07-16 | 2015-09-23 | 句容市容北茶文化有限公司 | Organic fertilizer for apocarya seedlings and preparation method of organic fertilizer |
| CN105085111A (en) * | 2015-08-12 | 2015-11-25 | 生之道生态农业科技股份有限公司 | Biological-organic fertilizer special for corn and preparation method thereof |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160427 |
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| RJ01 | Rejection of invention patent application after publication |