CN105519598A - Agent for preventing soft rot of Chinese cabbage - Google Patents
Agent for preventing soft rot of Chinese cabbage Download PDFInfo
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- CN105519598A CN105519598A CN201610102608.4A CN201610102608A CN105519598A CN 105519598 A CN105519598 A CN 105519598A CN 201610102608 A CN201610102608 A CN 201610102608A CN 105519598 A CN105519598 A CN 105519598A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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Abstract
The invention provides an active pharmaceutical ingredient for preventing soft rot of Chinese cabbage. The active pharmaceutical ingredient comprises a plant complex extracted component and streptomycin sulfate. By significant reduction of use of chemical antibiotics, drug residue is less, pollution to the environment is low, and control effect on soft rot is improved highly.
Description
Technical field
The present invention relates to biological pesticide technical field, particularly relating to a kind of medicament of the soft rot for preventing and treating Chinese cabbage.
Background technology
Chinese cabbage is rich in vitamin and mineral matter, can stir-fry and eat, make soup, pickled.Generally cultivate all over China.Chinese cabbage is known as " kings of hundred dishes ", has detoxication, and long-term edible being very beneficial for digests with healthy.Chinese cabbage occupies critical role in the vegetable basket project of northern China, and it can regulate dull season, stabilize dish valency.
Soft rot of cabbage is the disease that Chinese cabbage cultivation area generally occurs, and the popular time incidence of disease 30% ~ 40%, causes the significantly underproduction, and serious causes total crop failure.Soft rot of cabbage is not only serious in field harm, stores and transports and also can cause rotten in sales process, and loss greatly.
Current soft rot of cabbage is mainly based on chemical control.The prevention and controls of soft rot of cabbage is at premorbid or their early stage, to hocket foliage spray with dilutions such as streptomycin sulphate, new phytomycins.Although the effect of chemical control is very desirable, due to long-term use chemical pesticide, many side effects may be caused, the health of people is worked the mischief.
Along with improving constantly of living standards of the people, the continuous enhancing of consumption idea, the Chinese cabbage of production high-quality, low toxicity, low-residual meets needed for people's daily life, has seemed very important.This makes chemical control can not meet the requirement in epoch, find economical, effectively, the method for safe prevention vegetables germ and medicament become agricultural chemicals worker question of common concern.
Botanical Pesticide belongs to the one of biological agricultural chemicals, because of its active component easily degrade, residual low, the favor that the less pollution of environment is subject to day by day to people.
The application, by a large amount of screenings, obtains a kind of composite vegetable medicament, prepares with typical chemical antibiotics medicament, and obtain a kind of medicament for preventing and treating harm soft rot of cabbage, it substantially reduce the number the use of chemobiotic.Medicament residue is few, little to the pollution of environment, substantially increases the control efficiency of soft rot.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of medicament for preventing and treating harm soft rot of cabbage, and it substantially reduce the number the use of chemobiotic, and medicament residue is few, little to the pollution of environment, substantially increases the control efficiency of soft rot.
For solving the problems of the technologies described above, the invention provides a kind of active constituents of medicine for preventing and treating harm soft rot of cabbage, it comprises vegetalitas compound extract component and streptomycin sulphate.
Wherein, in the described active constituents of medicine for preventing and treating harm soft rot of cabbage, the weight portion of vegetalitas compound extract component and streptomycin sulphate is respectively vegetalitas composite insecticidal extract component 20 weight portion ~ 35 weight portion, streptomycin sulphate 2 weight portion ~ 3 weight portion.
Wherein, the raw medicinal material of described vegetalitas compound extract component comprises honeysuckle, erigeron breviscapus, persimmon leaf, the coptis, giant knotweed, radix ampelopsis, fructus ulmi, Chinese actinidia root, paniculate spotflower herb, rock spring grass, ivy, rabbit hair wormwood artemisia and Aster ageratoides.
Wherein, in described vegetalitas compound extract component, the weight portion of each raw medicinal material is respectively honeysuckle 30 weight portion ~ 45 weight portion, erigeron breviscapus 10 weight portion ~ 20 weight portion, persimmon leaf 15 weight portion ~ 25 weight portion, the coptis 35 weight portion ~ 50 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, radix ampelopsis 15 weight portion ~ 25 weight portion, fructus ulmi 10 weight portion ~ 20 weight portion, Chinese actinidia root 10 weight portion ~ 25 weight portion, paniculate spotflower herb 10 weight portion ~ 20 weight portion, rock spring grass 15 weight portion ~ 20 weight portions, ivy 10 weight portion ~ 15 weight portion, rabbit hair wormwood artemisia 10 weight portion ~ 20 weight portion and Aster ageratoides 10 weight portion ~ 20 weight portion.
The preparation method of described vegetalitas compound extract component is specially and gets each raw medicinal material, pulverize respectively, mix, 3h is extracted with the alcohol heat reflux that the determining alcohol relative to medicinal material mixture 10 ~ 12 times of quality is 90%, 3h is extracted again with the alcohol heat reflux that the determining alcohol of 8 ~ 10 times of quality is 90%, merge extract, being concentrated into relative density at 60 DEG C is the lotion of 1.15 ~ 1.20.
Wherein, the described medicament for preventing and treating harm soft rot of cabbage can be prepared into water dispersible granules, wetting powder, suspending agent, missible oil or microemulsion.
The present invention also provides a kind of wetting powder for preventing and treating harm soft rot of cabbage, and it comprises above-mentioned composite insecticide active component, dispersant, filler and wetting agent.
Wherein, in described wetting powder, the percentage by weight of each raw material is composite insecticide active component 30% ~ 60%, dispersant 1% ~ 10%, wetting agent 2% ~ 10%, and filler supplies 100%.
Present invention also offers the preparation method of above-mentioned wetting powder, each raw material is mixed in a mixer, mix again after airslide disintegrating mill is pulverized, namely can be made into wetting powder of the present invention.
Beneficial effect of the present invention:
The invention provides a kind of medicament for preventing and treating harm soft rot of cabbage, it substantially reduce the number the use of chemobiotic, and medicament residue is few, little to the pollution of environment, substantially increases the control efficiency of soft rot.
Embodiment
The invention provides a kind of active constituents of medicine for preventing and treating harm soft rot of cabbage, it comprises vegetalitas compound extract component and streptomycin sulphate.
In the described active constituents of medicine for preventing and treating harm soft rot of cabbage, the weight portion of vegetalitas compound extract component and streptomycin sulphate is respectively vegetalitas composite insecticidal extract component 20 weight portion ~ 35 weight portion, streptomycin sulphate 2 weight portion ~ 3 weight portion, further preferred plant composite insecticidal extract component 30 weight portion, streptomycin sulphate 2 weight portion.
This place's streptomycin sulphate can use commercially available streptomycin sulphate, and amount of application is in pure streptomycin sulphate.
The raw medicinal material of described vegetalitas compound extract component comprises honeysuckle, erigeron breviscapus, persimmon leaf, the coptis, giant knotweed, radix ampelopsis, fructus ulmi, Chinese actinidia root, paniculate spotflower herb, rock spring grass, ivy, rabbit hair wormwood artemisia and Aster ageratoides.
Further preferably, described vegetalitas compound extract component is only prepared from by above-mentioned raw materials medicinal material.
In described vegetalitas compound extract component, the weight portion of each raw medicinal material is respectively honeysuckle 30 weight portion ~ 45 weight portion, erigeron breviscapus 10 weight portion ~ 20 weight portion, persimmon leaf 15 weight portion ~ 25 weight portion, the coptis 35 weight portion ~ 50 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, radix ampelopsis 15 weight portion ~ 25 weight portion, fructus ulmi 10 weight portion ~ 20 weight portion, Chinese actinidia root 10 weight portion ~ 25 weight portion, paniculate spotflower herb 10 weight portion ~ 20 weight portion, rock spring grass 15 weight portion ~ 20 weight portions, ivy 10 weight portion ~ 15 weight portion, rabbit hair wormwood artemisia 10 weight portion ~ 20 weight portion and Aster ageratoides 10 weight portion ~ 20 weight portion.
Further preferably, in described vegetalitas compound extract component, the weight portion of each raw medicinal material is respectively honeysuckle 35 weight portion ~ 40 weight portion, erigeron breviscapus 15 weight portion ~ 20 weight portion, persimmon leaf 15 weight portion ~ 20 weight portion, the coptis 40 weight portion ~ 45 weight portion, giant knotweed 25 weight portion ~ 30 weight portion, radix ampelopsis 15 weight portion ~ 20 weight portion, fructus ulmi 10 weight portion ~ 15 weight portion, Chinese actinidia root 15 weight portion ~ 20 weight portion, paniculate spotflower herb 15 weight portion ~ 20 weight portion, rock spring grass 15 weight portion ~ 20 weight portions, ivy 10 weight portion ~ 15 weight portion, rabbit hair wormwood artemisia 10 weight portion ~ 15 weight portion and Aster ageratoides 15 weight portion ~ 20 weight portion.
Most preferably, in described vegetalitas compound extract component, the weight portion of each raw medicinal material is respectively honeysuckle 40 weight portion, erigeron breviscapus 15 weight portion, persimmon leaf 20 weight portion, the coptis 45 weight portion, giant knotweed 25 weight portion, radix ampelopsis 20 weight portion, fructus ulmi 15 weight portion, Chinese actinidia root 20 weight portion, paniculate spotflower herb 15 weight portion, rock spring grass 20 weight portions, ivy 10 weight portion, rabbit hair wormwood artemisia 15 weight portion and Aster ageratoides 15 weight portion.
Each raw medicinal material pharmacology is as follows:
Honeysuckle: latin name loniceraejaponicaeflos, taste is sweet, cold in nature, enters the heart, lung, stomach, large intestine channel, there is clearing heat and detoxicating effect, doublely to remove heat from the blood and relieve diarrhea, to be mainly used in affection of exogenous wind-heat, the hot poison of warm heat disease is comparatively contained, or when warm disease is still not descending at the part of the body cavity above the diaphragm housing the heart and lungs from the beginning of heat evil; The malicious diarrhea of heat, the lower blood of stool; Heat temperature is demonstrate,proved; The sore carbuncle that hot poison gathers from the beginning of, lift the heat person of swelling and ache, and other skin infections.
Erigeron breviscapus: pungent, the micro-hardship of taste, warm in nature, the thoughts of returning home, Liver Channel.There is expelling cold and relieving exterior syndrome, dispel rheumatism, effect of activating collaterals to relieve pain.For cold headache, toothache, stomachache, rheumatalgia pain, the paralysis that cerebrovas-cularaccident causes, osteomyelitis.
Persimmon leaf: taste is micro-sweet, bitter, cool in nature.Enter lung channel, energy clearing and antitussive, hemostasis, promotes the production of body fluid.For cough with lung heat; Bleeding from five sense organs or subcutaneous tissue, spitting of blood, spits blood, has blood in stool, uterine bleeding or menorrhalgia, hematuria, and purple plague purpura; Dysphoria with smothery sensation is thirsty.
The coptis: bitter, cold in nature, enter the heart, lung, courage, stomach, large intestine channel, have heat-clearing and damp-drying drug, purging intense heat and detonicating, the effect of cooling blood for improving eyesight, cures mainly damp and hot ruffian full, vomiting acid regurgitation, rush down dysentery, jaundice, unconsciousness due to high fever, heart-fire hyperactivity, dysphoria and insomnia, blood-head tells nosebleed, hot eyes, toothache, quenches one's thirst, and carbuncle swells furunculosis, eczema, wet sore, duct such as to be suppurated at the card.
Giant knotweed: mildly bitter flavor, cold nature, returns liver, courage, lung channel, has clearing heat and detoxicating, normalizing gallbladder to cure jaundice, expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum effect, for joint pain, jaundice with damp-heat pathogen, through closing, leprosy lump in the abdomen, coughing with a lot of sputum, scald, injury from falling down, the cards such as carbuncle sore tumefacting virus.
Radix ampelopsis: latin name AmpelopsisRadix, bitter, cold nature, the thoughts of returning home, stomach warp, have clearing heat and detoxicating, the carbuncle that disappears dissipating bind, expelling pus and promoting granulation effect, cure mainly the cards such as ulcer carbuncle on the back, furunculosis, burn and scald.
Fructus ulmi: taste is pungent, bitter, warm in nature.Return spleen, stomach warp, there is effect of killing insect and eliminating accumulation, for infantile malnutrition, roundworm disease, enterobiasis.
Chinese actinidia root: latin name Actinidiaarguta (Sieb. & Zucc) Planch.exMiq., taste is sour, puckery, cool in nature, have clearing heat and detoxicating, effect of clearing heat and promoting diuresis, preventing tumor is anticancer, dispel rheumatism, diuresis is stopped blooding, removing toxicity for detumescence, the cards such as indigestion, vomiting, arthralgia due to wind-dampness, treating rheumatic ostealgia, Alimentary Tract Cancer, tumor in digestive tract, large carbuncle sore furuncle, treating rheumatic ostealgia and jaundice.
Paniculate spotflower herb: warm in nature, taste is pungent, nontoxic, detoxification and promoting urination, relieving cough and relieving asthma, swelling and pain relieving.For malaria, toothache, enteritis, dysentery, cough, asthma, pertussis, pulmonary tuberculosis, the cards such as venomous snake bite is controlled in external application, dog bite, carbuncle furuncle.
Rock spring grass: be the herb of Aspleniaceae spleen wort tiger tail maidenhair spleenwort, bitter, sweet, cool in nature, have clearing heat and detoxicating, flat liver, effect of diuresis, cures mainly jaundice with damp-heat pathogen, cough with lung heat, child convulsion, difficult urination, the cards such as felon.
Ivy: taste is pungent, bitter, property is put down, and returns liver, spleen, lung channel, has and dispel the wind, dampness removing, and blood, and effect of removing toxic substances, cures mainly arthralgia due to wind-dampness, headache, dizzy, hepatitis, traumatic injury, abscess of throat, and carbuncle swells the cards such as streamer, snake bite and insect sting.
Rabbit hair wormwood artemisia: be the herb of composite family line Dendranthema Filifolium sibiricum, bitter, cold in nature, have clearing heat and detoxicating, anti-inflammation, calm the nerves relieving convulsion, effect of regulating menstruation, hemostasis, cure mainly the high heat of infectious disease, heartbeat, insomnia, neurasthenia, menorrhalgia, the cards such as irregular menstruation.
Aster ageratoides: be herb or the root of composite family Aster plant three arteries and veins aster, bitter, pungent, cool in nature, have clearing heat and detoxicating, the effect of the cool blood that eliminates the phlegm, cure mainly cold, fever, tonsillitis, bronchitis, hepatitis, enteritis, dysentery, heat pouring, blood-head, the cards such as worm snake bite.
The preparation method of described vegetalitas compound extract component is specially and gets each raw medicinal material, pulverize respectively, mix, 3h is extracted with the alcohol heat reflux that the determining alcohol relative to medicinal material mixture 10 ~ 12 times of quality is 90%, 3h is extracted again with the alcohol heat reflux that the determining alcohol of 8 ~ 10 times of quality is 90%, merge extract, being concentrated into relative density at 60 DEG C is the lotion of 1.15 ~ 1.20.
The described medicament for preventing and treating harm soft rot of cabbage can be prepared into water dispersible granules, wetting powder, suspending agent, missible oil or microemulsion, is preferably prepared into wetting powder.
The present invention also provides a kind of wetting powder for preventing and treating harm soft rot of cabbage, and it comprises above-mentioned composite insecticide active component, dispersant, filler and wetting agent.
Further preferably, described wetting powder is only prepared from by above-mentioned raw materials.
In described wetting powder, the percentage by weight of each raw material is composite insecticide active component 30% ~ 60%, dispersant 1% ~ 10%, wetting agent 2% ~ 10%, and filler supplies 100%.
Further preferably, in described wetting powder, the percentage by weight of each raw material is composite insecticide active component 40% ~ 60%, dispersant 2% ~ 6%, wetting agent 4% ~ 7%, and filler supplies 100%.
Present invention also offers the preparation method of above-mentioned wetting powder, each raw material is mixed in a mixer, mix again after airslide disintegrating mill is pulverized, namely can be made into wetting powder of the present invention.
Described filler is the mixture of one or more in bentonite, imvite, common brick, clay, slag kaolin, diatomite, white carbon, calcium dodecyl benzene sulfonate.
Described dispersant can be the mixture of one or more in polycarboxylate, lignosulfonates.
Described wetting agent can be sulphate, one or more mixing of sulfonate.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 vegetalitas composite insecticidal extract component
Extracting honeysuckle 40g, erigeron breviscapus 15g, persimmon leaf 20g, coptis 45g, giant knotweed 25g, radix ampelopsis 20g, fructus ulmi 15g, Chinese actinidia root 20g, paniculate spotflower herb 15g, the rock spring grass 20g, ivy 10g, rabbit hair wormwood artemisia 15g and Aster ageratoides 15g, pulverize respectively, mix, 3h is extracted with the alcohol heat reflux that the determining alcohol of 3kg is 90%, 3h is extracted again with the alcohol heat reflux that the determining alcohol of 2.5kg is 90%, merge extract, being concentrated into relative density at 60 DEG C is the lotion of 1.20.
Embodiment 2 composite insecticidal active component
Get 20g streptomycin sulphate and (get the streptomycin sulphate wetting powder of 72% of North China pharmaceutical Co. Ltd production, converting by former medicine 20g streptomycin sulphate and take corresponding weight) the vegetalitas composite insecticidal extract component prepare with 300g embodiment 1 is uniformly mixed, acquisition composite insecticidal active component.
Embodiment 3 wetting powder 1
Composite insecticidal active component 500g prepared by embodiment 2, dispersant lignin calcium carbonate 60g, wetting agent CB-102 (diisooctyl succinate sulfonate) 40g, filler diatomite 300g, white carbon 100g mix in a mixer, mix again after airslide disintegrating mill is pulverized, namely can be made into wetting powder.
Embodiment 4 wetting powder 2
Vegetalitas composite insecticidal extract component 500g prepared by embodiment 1, dispersant lignin calcium carbonate 60g, wetting agent CB-102 (diisooctyl succinate sulfonate) 40g, filler diatomite 300g, white carbon 100g mix in a mixer, mix again after airslide disintegrating mill is pulverized, namely can be made into wetting powder.
The indoor independent mensuration of embodiment 5
Test medicine: North China pharmaceutical Co. Ltd produce 72% streptomycin sulphate wetting powder, embodiment 3 prepare wetting powder 1, embodiment 4 prepare wetting powder 2.
For examination pathogen
Erwinia carotorora (Erwiniacarotorora) plants disease laboratory by plant protection institute of Qingdao Agricultural University to be provided.
Indoor biometrics method
Indoor biometrics adopts inhibition zone method.Through prerun, finally determine that 100mg/L, 75mg/L, 50mg/L, 40mg/L, 25mg/L five concentration process established by the streptomycin sulphate wetting powder of 72%, 100mg/L, 75mg/L, 50mg/L, 40mg/L, 25mg/L five concentration process established by wetting powder 1,150mg/L, 125mg/L, 100mg/L, 75mg/L, 50mg/L five concentration process established by wetting powder 2, be all contrast with sterile water, often process repetition 3 times.
For the preparation of examination bacterial classification
Will for examination bacterial classification before the test switching activation in 2 days to be placed in 28 DEG C of insulating boxs for subsequent use.
The preparation of bacterial suspension
Bacterial classification cultured in test tube is extracted test tube tampon near alcolhol burner place, pour 10mL sterile water into, with the transfer needle of sterilizing, the germ on inclined-plane is scraped gently, make spore suspension, being poured into by this spore suspension is equipped with in the sterilizing triangular flask of several glass marbles in advance again, cross bacteriological filtration liquid with sterile gauze after shake 5min, put in another sterilizing triangular flask, namely make required bacterium liquid.
With the preparation of bacterium culture medium
By beef extract-peptone agar medium melt, to be cooled to 45 ~ 50 DEG C time, aseptically pour into rapidly in sterilizing culture dish together with 0.2mL bacterial suspension, every ware 15mL medium, shakes up rear cooled and solidified.With the card punch punching after sterilizing, 1mL liquid is dripped in circular hole, is placed in 28 DEG C of insulating boxs and cultivates 2d, measure 2 diameters of inhibition zone with slide measure by right-angled intersection method, average.With antibacterial circle diameter square for ordinate, the logarithm (1gc) of drug concentration is abscissa, obtains virulence curve, obtains EC50 with method of least squares.
The synergistic effect of pharmacy mix is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC≤80 are antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect:
Toxicity index (TI)=(EC of standard agent
50the EC of/reagent agent
50) × 100;
The actual toxicity index of mixture (ATI)=(EC that standard agent is alone
50the EC of/mixture
50) × 100;
Theoretical toxicity index (TTI)=ATI (A) × A percentage+ATI (the B) × B percentage in mixture in mixture of mixture;
Co-toxicity coefficient (CTC)=[the theoretical toxicity index (TTI) of the actual toxicity index of mixture (ATI)/mixture] × 100;
If CTC≤80, Mixed Pharmacy is antagonism; If 80 < CTC < 120, Mixed Pharmacy is summation action; If CTC > 120, Mixed Pharmacy is synergistic effect.
The toxicity test index of table 1 different agents compares
Embodiment 6 field control effectiveness test
Crop varieties: Jiangzhou, Qingdao Chinese cabbage.
Environmental condition: be selected in pollution-free vegetable production base for examination plot, selected plot crop-planting kind is comparatively single, and that a situation arises is comparatively serious for Chinese cabbage disease.Plot, each experimental plot is smooth, and implantation time, growth situation, Cultivate administration method and the measures situation of plant are all consistent.
Test medicine: North China pharmaceutical Co. Ltd produce 72% streptomycin sulphate wetting powder, embodiment 3 prepare wetting powder 1, embodiment 4 prepare wetting powder 2, clear water (blank/CK).
Liquid proportion: streptomycin sulphate wetting powder 4000 times of liquid of test employing 72%, wetting powder 14000 times of liquid prepared by embodiment 3, wetting powder 24000 times of liquid prepared by embodiment 4.
Community is arranged: the area of community is 70m
2, establish 5 process altogether, each process repetition 3 times, therefore establish 15 experimental plots altogether, the selection of dispenser community is adopted to the mode of randomized arrangement.Community words spoken by an actor from offstage colza planting distance is 35cm × 50cm (spacing in the rows × line-spacing) from arrangement, and plant spacing is converted into 3700 strains/667m
2, and be provided with more than 10m protection row in experimental plot surrounding.
Test process: 1) processing procedure: after Chinese cabbage final singling Yu after entering bag heart stage, dispenser process is carried out to the plant in each experimental plot respectively.Carry out drug solution preparing according to operation instruction and select the sunny community of calm weather to randomized arrangement to spray corresponding liquid or clear water, continuous use twice, and the time interval is 7 days.Utilize knapsack sprayer to carry out routine spraying to Chinese cabbage leaf tow sides in wherein dispenser process, even spray liquid is to Chinese cabbage phyllopodium part is drenched.
2) prevent and treat the medicament data of other damage by disease and insect: at duration of test, affect result of the test for preventing interaction between medicine, do not use other medicaments in examination.
Investigation method, time and number of times
1) investigation method: before dispenser, first carries out investigation records to the plant situation of catching an illness in experimental plot, understands its state of an illness radix.Add up the incidence of Chinese cabbage in experimental plot respectively after 7 days in first time and second time dispenser process, adopt diagonal five point sampling in fact-finding process, often some quantity of sampling quantity is 15 strains.Record its plant sum, infected plant number and coincident with severity degree of condition, calculate its diseased plant rate, disease index and control efficiency, and its significant difference is analyzed.In addition, after dispenser process, answer close observation and record Chinese cabbage and whether occur bad poisoning phenomenon.
Disease index for reference, is divided into 5 grades: 0 grade: without scab with disease scale standard altogether; 1 grade: lesion area accounts for less than 5% of whole leaf area; 2 grades: lesion area accounts for 6% ~ 10% of whole leaf area; 3 grades: lesion area accounts for 11% ~ 20% of whole leaf area; 4 grades: lesion area accounts for 21% ~ 50% of whole leaf area; 5 grades: lesion area accounts for more than 50% of whole leaf area.
Control time and number of times: three investigation are carried out in test altogether: before dispenser first, do first time investigation, record plant sum and incidence; Second time investigation was done, catch an illness quantity and poisoning situation after the dispenser first of record plant before secondary dispenser; Within the 7th day after secondary dispenser, do third time investigation, catch an illness quantity and poisoning situation after the dispenser of record plant secondary.
Drug effect computational methods
Diseased plant rate (%)=diseased plant number/investigate total strain number × 100;
Disease index (%)=(∑ diseased plant at different levels number × typical value at different levels)/(the highest typical value × investigate total strain number) × 100;
Control efficiency (%)=(check plot diseased plant rate-treatment region diseased plant rate)/diseased plant rate × 100, check plot [
Results and analysis
Different disposal is on the impact of crop safety: in process of the test, respectively process community plant strain growth situation to field observe.Range estimation display: after dispenser, the abnormal conditions such as leaf abscission, wilting variable color, developmental deformity do not occur plant, illustrate thus under set experimental condition, institute's with medicament does not produce bad poisoning impact to Chinese cabbage.
Different disposal, on the impact of Chinese cabbage output, the results are shown in Table 2.
Table 2 different disposal is on the impact of Chinese cabbage output
In process of the test, related data is gathered and record, obtained the effect of each chemical control soft rot of cabbage by process.As can be seen from Table 2, use wetting powder 1 and wetting powder 2 to carry out the community prevented and treated, the growth situation of Chinese cabbage and output are all better than streptomycin sulphate, and the production-increasing function that wetting powder 1 shows is the most remarkable.
Different disposal control efficiency affects, and the results are shown in Table 3.
Table 3 different disposal control efficiency affects
Reagent agent in table 3 carries out dispenser process to test region Chinese cabbage respectively, though all reach certain control efficiency, degree varies with bag heart stage within the scope of operation instruction specified volume after final singling.Control efficiency after the dispenser of streptomycin secondary is poor compared with first time, and reason is to test in the process of preventing and treating soft rot of cabbage before plot, and the use of streptomycin is comparatively frequent, and this germ has created certain drug resistance.And wetting powder 1 and wetting powder 2 after final singling with bag heart stage administered twice after control efficiency all better, control efficiency is good, shows good growth promoting function.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (9)
1., for preventing and treating an active constituents of medicine for harm soft rot of cabbage, it is characterized in that, comprise: vegetalitas compound extract component and streptomycin sulphate.
2., as claimed in claim 1 for preventing and treating the active constituents of medicine of harm soft rot of cabbage, it is characterized in that: the weight portion of described vegetalitas compound extract component and described streptomycin sulphate is respectively vegetalitas composite insecticidal extract component 20 weight portion ~ 35 weight portion, streptomycin sulphate 2 weight portion ~ 3 weight portion.
3., as claimed in claim 1 or 2 for preventing and treating the active constituents of medicine of harm soft rot of cabbage, it is characterized in that: the raw medicinal material of described vegetalitas compound extract component comprises honeysuckle, erigeron breviscapus, persimmon leaf, the coptis, giant knotweed, radix ampelopsis, fructus ulmi, Chinese actinidia root, paniculate spotflower herb, rock spring grass, ivy, rabbit hair wormwood artemisia and Aster ageratoides.
4. the active constituents of medicine for preventing and treating harm soft rot of cabbage as described in claims 1 to 3, it is characterized in that: in described vegetalitas compound extract component, the weight portion of each raw medicinal material is respectively honeysuckle 30 weight portion ~ 45 weight portion, erigeron breviscapus 10 weight portion ~ 20 weight portion, persimmon leaf 15 weight portion ~ 25 weight portion, the coptis 35 weight portion ~ 50 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, radix ampelopsis 15 weight portion ~ 25 weight portion, fructus ulmi 10 weight portion ~ 20 weight portion, Chinese actinidia root 10 weight portion ~ 25 weight portion, paniculate spotflower herb 10 weight portion ~ 20 weight portion, rock spring grass 15 weight portion ~ 20 weight portions, ivy 10 weight portion ~ 15 weight portion, rabbit hair wormwood artemisia 10 weight portion ~ 20 weight portion and Aster ageratoides 10 weight portion ~ 20 weight portion.
5. the active constituents of medicine for preventing and treating harm soft rot of cabbage as described in Claims 1-4, it is characterized in that: the preparation method of described vegetalitas compound extract component is specially and gets each raw medicinal material, pulverize respectively, mix, 3h is extracted with the alcohol heat reflux that the determining alcohol relative to medicinal material mixture 10 ~ 12 times of quality is 90%, 3h is extracted again with the alcohol heat reflux that the determining alcohol of 8 ~ 10 times of quality is 90%, merge extract, being concentrated into relative density at 60 DEG C is the lotion of 1.15 ~ 1.20.
6., for preventing and treating a wetting powder for harm soft rot of cabbage, it is characterized in that: comprise composite insecticide active component, dispersant, filler and the wetting agent described in claim 1 to 5.
7. described in claim 6 for prevent and treat harm soft rot of cabbage wetting powder, it is characterized in that: the percentage by weight of each raw material is composite insecticide active component 30% ~ 60%, dispersant 1% ~ 10%, wetting agent 2% ~ 10%, and filler supplies 100%.
8. the preparation method of wetting powder described in claim 6 or 7, is characterized in that: mixed in a mixer by each raw material, mixes again, namely can be made into wetting powder of the present invention after airslide disintegrating mill is pulverized.
9. the application of composite insecticide active component described in Claims 1-4 in control harm soft rot of cabbage.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610102608.4A CN105519598A (en) | 2016-02-25 | 2016-02-25 | Agent for preventing soft rot of Chinese cabbage |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610102608.4A CN105519598A (en) | 2016-02-25 | 2016-02-25 | Agent for preventing soft rot of Chinese cabbage |
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| CN201610102608.4A Pending CN105519598A (en) | 2016-02-25 | 2016-02-25 | Agent for preventing soft rot of Chinese cabbage |
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Application publication date: 20160427 |