CN105494054A - Method for potato virus-free breeding - Google Patents
Method for potato virus-free breeding Download PDFInfo
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- CN105494054A CN105494054A CN201410496918.XA CN201410496918A CN105494054A CN 105494054 A CN105494054 A CN 105494054A CN 201410496918 A CN201410496918 A CN 201410496918A CN 105494054 A CN105494054 A CN 105494054A
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Abstract
The invention relates to a method for potato virus-free breeding. The method comprises steps: screening a substrate and disinfecting; cleaning and disinfecting a seedbed or a seedling bed and a net shed; transplanting; about one month after planting of virus-free seedlings, cutting seedling tips to produce cutting seedlings, and performing normalized control. The method does not have special requirements on varieties, and is especially suitable to be used for mini species. The method greatly reduces production cost, and improves breeding speed. Survival rate of the cutting seedlings reaches up to more than 95%, and yield is greatly increased, and generally the method increases yield by more than 50%. The method reduces application of pesticides, and is beneficial for protecting ecological environment and ensuring food safety.
Description
Technical field
The present invention relates to crops and breed field, particularly relate to potato detoxicating and breed field.
Background technology
Potato is a kind of special crop, because the harm kind sexual involution of virus is very fast, therefore can not reserve seed for planting in warm area, otherwise by the significantly underproduction.Recently, developed country is universal virus-free seed potato in potato produces, and be multiplied than the traditional planting patterns output of employing, benefit significantly improves.Though China begins one's study from the seventies in last century, promotes virus-free seed potato; But apply area so far and only have about 10%, studying carefully its main cause is that virus-free seed potato production exists technical bottleneck.Average per unit area yield only reaches 1/3rd of European and American developed countries though China is potato producing country maximum in the world! On the one hand, existing potato generally adopts the stripping and slicing of potato block to sow, and this makes cost very high; On the other hand, although it is a kind of more advanced mating system that existing mist training method potato detoxicating is bred, but this method can not make full use of the spaces such as greenhouse, cost is still very high, simultaneously will to gathering in the crops toxicity-removing white potato in time, need a large amount of human users, virtually turn increase management cost, reduce production efficiency.Therefore, the problem of breeding solving virus-free seed potato is that current China popularizes virus-free seed potato application, promotes the key issue that the potato level of production improves.
Summary of the invention
The present invention, to solve the problem in above-mentioned background technology, makes full use of the growth and development characteristics of the lower rule of plant stem apex virus concentration and potato, provides a kind of method that potato detoxicating is bred.
Technical problem solved by the invention realizes by the following technical solutions:
The invention provides a kind of method that potato detoxicating is bred, the method is carried out according to the following steps:
1) screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the transplanting medium of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
2) cleaning of seedbed or seedling furrow and net canopy and sterilization: before transplanting 2-3 days to net canopy space disinfection, kill various worm's ovum, and at laid inside cloth or plastic foil, be layered on described cloth or plastic foil after matrix sterilization;
3) transplant: select optimum transplanting to transplant opportunity, and when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection; Now to note not damaging root system and cauline leaf, avoid courses of infection lethal.
4) detoxic seedling field planting about month, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, and the number of times after this surely watered according to the actual conditions of seedling every day and amount, control the wet poison of air by micro-spray or adopt plastics Small plastic shed to improve air wet malicious;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling transplant after in 15-20 days, in net canopy and Small plastic shed, temperature should control within 30 degrees Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 7-10 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 15-20 days;
(5) fertilizer controls: after detoxic seedling field planting 3-5 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly.
In the present invention, wherein said transplanting medium, for have good compacting covering effect to stolon, especially, is vermiculite.
Transplant the matrix of detoxic seedling, be usually can transplant successfully with the key of growth of seedling quality
In the present invention, wherein 1) the thorough disinfection method described in, i.e. 28-35 days before transplanting, water with formaldehyde 100 times of liquid of 38%-45% and drench matrix in seedbed or seedling furrow, then covering with plastic film 7-10 days, within after taking off film 10-15 days, transplant detoxic seedling, period digs matrix at least 1-2 time again, and formaldehyde odor is volatilized as early as possible.Be generally in the transplanting previous moon, use formaldehyde 100 times of liquid of percent 40.
In the present invention, wherein 2) cloth described in or plastic foil need sterilization and disinsection before use, and the stromal thickness on described cloth or plastic foil is 7-9 cm.Optimum thickness is 8 centimetres.The cloth used is color cloth.
Before transplanting, the sterilization of cleaning seedbed or seedling furrow and net canopy, is to provide one of requisite measure of potato tissue culture transplantation of seedlings survival rate.If do not carried out disinfection, then the tender detoxic seedling of the children grown under aseptic conditions easily infects various germ after transplanting, and causes death.
In the present invention, wherein 2) described in, net canopy space is carried out disinfection, use flolimat or phoxim baythion 1000 times of liquid.
In the present invention, wherein 3) transplanting described in is opportunity, after the expansion of potato virus-free plantlet subculture is numerous, about 18-20 days are grown in blake bottle, now height of seedling 6-8 centimetre, every young plant has root 5-8 bar, and the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter.
If seedling is too short for vegetative period in blake bottle, then seedling is thin and delicate short and small, unmanageable after transplanting, easily dead.On the contrary, in blake bottle, vegetative period is long, then seedling is too high, this section easy when transplanting, and root system is long, and browning is aging, is unfavorable on the contrary surviving.
In the present invention, wherein 3) root described in carries out disinfection, and uses potassium permanganate dilution, root-inducing powder and bactericide mixed diluting liquid to do root and disinfects.
In method described in the present invention, the whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, regularly, especially, within 7-10 days, carries out injection sterilization, insecticide; Especially, carbendazim 800, Sandofan 1000 times of liquid, Avermectin 1500 times of liquid, aphid difficult to understand 1500 times of liquid etc. can be used, be successively used alternatingly.
In the present invention, wherein 4) illumination described in controls, specifically refer to the sunshade net drawing 1-2 layer shading rate more than 60% outside net canopy, according to the recovery situation of seedling and survival rate after 10-15 days, progressively strengthen illumination, until seedling complete reform of nature environmental condition the dismountable sunshade measure in rear of looking sturdy.
If find virus, then carry out Viral diagnosis to being used for cutting the potato virus-free plantlet that point breeds, if find virus, then detoxic seedling no longer squamous subculture, even destroys, and field seed selection again, carries out stem apex stripping.Now, check farm record, see cultivation alleviates whether have careless mistake, to correcting.
Maintenance water balance and humidity of controlling well are the cores of potato virus-free plantlet transplanting and management technology.Air appropriateness is low, then detoxic seedling is easily wilted; And water supply in media is too much unsuitable, if water supply in media is too many, not only ventilative bad, impact is taken root, and easily causes rotten dead seedling.Therefore, while guarantee air humidity is enough large, the ventilative situation that transplanting medium is good should be guaranteed as far as possible.
Illumination controls also very important.If illumination is excessively strong, the chlorophyll of seedling can be made to be damaged, and photosynthesis weakens, and leaf chlorosis, turns to be yellow or turns white, and seedling is survived and delays; Illumination is simultaneously crossed and transpiration effect can be made by force to strengthen, and make water balance contradiction more sharp-pointed, the easy dehydration of plant is wilted dead.
Meanwhile, because potato is ice-cold crop, the accommodation for temperature is comparatively wide, if but ground temperature is lower, and slow seedling is comparatively slow, and seedling growth retardation, then have certain influence to survival rate; And at summer high temperature, after detoxic seedling is transplanted, the temperature controlled well in net canopy, temperature is unsuitable too high, because hot and humid environment very easily infects death, meanwhile, temperature is too high, and the transpiration of plant is strengthened, and easy dehydration causes death, thus affects transplanting survival rate.
Beneficial effect of the present invention is:
1. pair kind is without particular/special requirement, is particularly useful for miniature kind;
2. greatly reduce production cost, improve the speed of breeding;
3. cuttage seeding survival rate is up to more than 95%, greatly adds output, general volume increase more than 50%;
4. can reduce the applications of pesticide, to protecting ecology, environmental benefits, favourable to ensuring food safety.
Embodiment
Embodiment 1:
The method that potato detoxicating is bred, the method is carried out according to the following steps:
1. the screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the vermiculite of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
2. the cleaning of seedbed and net canopy and sterilization: transplant first 2 days to net canopy space disinfection, kill various worm's ovum, and at laid inside cloth, after matrix sterilization, be layered on described cloth;
3. transplant: select optimum transplanting to transplant opportunity, namely in blake bottle, grow 18 days, now height of seedling 6-8 centimetre, every young plant has root 5-8 bar, and the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter.And when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection;
4. detoxic seedling field planting one month, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, and the number of times after this surely watered according to the actual conditions of seedling every day and amount, adopt plastics Small plastic shed to improve the wet poison of air;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling is transplanted in latter 15 days, and in net canopy and Small plastic shed, temperature should control between 30-20 degree Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 7 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 15 days;
(5) fertilizer controls: after detoxic seedling field planting 3 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly.
The whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, regularly, namely within 7-10 days, carries out injection sterilization, insecticide; Especially, use carbendazim 800, Sandofan 1000 times of liquid, Avermectin 1500 times of liquid, aphid difficult to understand 1500 times of liquid etc., be successively used alternatingly.
Embodiment 2:
The method that potato detoxicating is bred, the method is carried out according to the following steps:
1. the screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the transplanting medium of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
2. the cleaning of seedling furrow and net canopy and sterilization: transplant first 3 days to net canopy space disinfection, kill various worm's ovum, and at laid inside plastic foil, be layered on described plastic foil after matrix sterilization;
3. transplant: select optimum transplanting to transplant opportunity, i.e. height of seedling 6-8 centimetre, time every young plant has a root 5-8 bar, now the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter.And when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection;
4. detoxic seedling field planting 30 days, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, the number of times after this surely watered according to the actual conditions of seedling every day and amount, and controlling the wet poison of air by micro-spray, to improve air wet malicious;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling is transplanted in latter 20 days, and in net canopy and Small plastic shed, temperature should control between 25-18 degree Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 10 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 20 days;
(5) fertilizer controls: after detoxic seedling field planting 5 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly.
Wherein 1) the thorough disinfection method described in, namely in transplanting first 30 days, the 100 times of liquid of the formaldehyde with 42% watered and drench matrix, then covering with plastic film 10 days in seedling furrow, and within after taking off film 15 days, transplant detoxic seedling, period digs matrix 2 times again, and formaldehyde odor is volatilized as early as possible.
Wherein 2) plastic foil described in needs sterilization and disinsection before use, and the stromal thickness on described plastic foil is 7 cm.
Wherein 2) described in, net canopy space is carried out disinfection, use flolimat.
Wherein 3) root described in carries out disinfection, and uses potassium permanganate dilution, root-inducing powder and bactericide mixed diluting liquid to do root and disinfects.
The whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, within every 7 days, carries out injection sterilization, insecticide; With carbendazim 800, Sandofan 1000 times of liquid, Avermectin 1500 times of liquid, aphid difficult to understand 1500 times of liquid, be successively used alternatingly.
Embodiment 3:
The method that potato detoxicating is bred, the method is carried out according to the following steps:
1. the screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the transplanting medium of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
2. the cleaning of seedling furrow and net canopy and sterilization: transplant first 2 days to net canopy space disinfection, kill various worm's ovum, and at the colored cloth of laid inside, be layered on described colored cloth after matrix sterilization;
3. transplant: select optimum transplanting to transplant opportunity, and when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection;
4. detoxic seedling field planting 32 days, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, and the number of times after this surely watered according to the actual conditions of seedling every day and amount, control the wet poison of air by micro-spray or adopt plastics Small plastic shed to improve air wet malicious;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling is transplanted in latter 18 days, and in net canopy and Small plastic shed, temperature should control between 30-18 degree Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 9 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 18 days;
(5) fertilizer controls: after detoxic seedling field planting 4 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly.
Wherein said transplanting medium, for have good compacting covering effect to stolon, is vermiculite.
Wherein 1) the thorough disinfection method described in, namely in transplanting first 30 days, the 100 times of liquid of the formaldehyde with 40% watered and drench matrix in seedbed or seedling furrow, then covering with plastic film 8 days, within after taking off film 13 days, transplant detoxic seedling, period digs matrix at least 1-2 time again, and formaldehyde odor is volatilized as early as possible.
Wherein 2) the colored cloth described in needs sterilization and disinsection before use, and the stromal thickness on described colored cloth is 7-9 cm.
Wherein 2) described in, net canopy space is carried out disinfection, use flolimat.
Wherein 3) transplanting described in is opportunity, potato virus-free plantlet subculture expand numerous after, in blake bottle, grow 19 days, now height of seedling 6-8 centimetre, every young plant has root 5-8 bar, and the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter.
Wherein 3) root described in carries out disinfection, and uses potassium permanganate dilution, root-inducing powder and bactericide mixed diluting liquid to do root and disinfects.
In described method, the whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, regularly, especially, within 8 days, carries out injection sterilization, insecticide; Especially, use carbendazim 800, Sandofan 1000 times of liquid, aphid difficult to understand 1500 times of liquid, be successively used alternatingly.
Wherein 4) illumination described in controls, specifically refer to the sunshade net drawing 1 layer of shading rate 60% outside net canopy, according to the recovery situation of seedling and survival rate after 10 days, progressively strengthen illumination, until seedling complete reform of nature environmental condition the dismountable sunshade measure in rear of looking sturdy.
Embodiment 4:
The method that potato detoxicating is bred, the method is carried out according to the following steps:
1. the screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the transplanting medium of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
2. the cleaning of seedbed and net canopy and sterilization: transplant first 3 days to net canopy space disinfection, kill various worm's ovum, and at laid inside plastic foil, be layered on described plastic foil after matrix sterilization;
3. transplant: select optimum transplanting to transplant opportunity, and when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection;
4. detoxic seedling field planting 31 days, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, and the number of times after this surely watered according to the actual conditions of seedling every day and amount, control the wet poison of air by micro-spray or adopt plastics Small plastic shed to improve air wet malicious;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling is transplanted in latter 19 days, and in net canopy and Small plastic shed, temperature should control between 28-20 degree Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 9 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 17 days;
(5) fertilizer controls: after detoxic seedling field planting 5 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly.
Wherein 1) the thorough disinfection method described in, namely in transplanting first 33 days, the 100 times of liquid of the formaldehyde with 42% watered and drench matrix, then covering with plastic film 9 days in seedbed and seedling furrow, and within after taking off film 13 days, transplant detoxic seedling, period digs matrix again, and formaldehyde odor is volatilized as early as possible.
Wherein 2) plastic foil described in needs sterilization and disinsection before use, and the stromal thickness on described plastic foil is 7 centimetres.
Wherein 2) described in, net canopy space is carried out disinfection, use flolimat and phoxim baythion 1000 times of liquid.
Wherein 3) transplanting described in is opportunity, potato virus-free plantlet subculture expand numerous after, in blake bottle, grow about 17 days, now height of seedling 6-8 centimetre, every young plant has root 5-8 bar, and the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter.
Wherein 3) root described in carries out disinfection, and uses potassium permanganate dilution, root-inducing powder and bactericide mixed diluting liquid to do root and disinfects.
In described method, the whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, regularly carries out injection sterilization, insecticide; Use carbendazim 800, Avermectin 1500 times of liquid, aphid difficult to understand 1500 times of liquid, be successively used alternatingly.
Wherein 4) illumination described in controls, specifically refer to the sunshade net drawing 2 layers of shading rate 75% outside net canopy, according to the recovery situation of seedling and survival rate after 11 days, progressively strengthen illumination, until seedling complete reform of nature environmental condition the dismountable sunshade measure in rear of looking sturdy.
Above embodiment, as long as ensure that humidity remains on more than 85%, within general 7-10 days, can take root, survival rate is up to more than 95%.Every 20 days, carry out sampling Detection to cutting the potato set that point breeds; If find that there is the malicious seedling of band, must trace band toxogen because of, propose solution and also destroy this collection of seedling.
Through experiment, in potato miniature seed production process, every strain detoxic seedling cost is 0.25 yuan, and speed of breeding in the past is slow, and growth cycle is 90-120 days, virtually adds production cost.Method of the present invention, not only decrease the consumption of potato virus-free plantlet, and the group training room area of breeding potato virus-free micro-seed use can reduce 80%, growth cycle is 70 days, what improve virus-free seed potato breeds speed, reduces the production cost of virus-free seed potato, volume increase 50%, cost declines 30%, and virus-free seed potato price can be made significantly to reduce.By demonstration area (20000 square metres) and the 0.30 yuan of calculating of every virus-free miniature potato seed cost, the comparable existing routine techniques of this new technology is adopted to save cost about 900,000 yuan; If produce potato virus-free micro-seed 300,000,000 Granule Computing at present per year by China, all adopt this new technology to produce, cost-saved about 2,700 ten thousand yuan every year, the fund turnover period of manufacturing enterprise can be made about to shorten one times simultaneously
And the existing potato planting area of China more than 8,000 ten thousand mu (this be statistics area, nearly 100,000,000 mu of real area possibility), only have an appointment and 10% have employed real virus-free seed potato, if all adopt virus-free seed potato to produce, potato more than 4,000 ten thousand tons can be increased production every year, be equivalent to produce more food more than 8,000,000 tons, this is to ensureing that the staple food supply of China is significant; If potato price, by 1.00 yuan of/kilogram of calculating, namely can be peasant and increases income more than 40,000,000,000 yuan! Application virus-free seed potato can also reduce potato disease, not only saves agricultural chemicals and drops into, the more important thing is and decrease environmental pollution because decreasing the applications of pesticide, protect ecology, be more conducive to food security.
Utilize technical solutions according to the invention, or those skilled in the art being under the inspiration of technical solution of the present invention, designing similar technical scheme, and reach above-mentioned technique effect, is all fall into protection scope of the present invention.
Claims (9)
1. the potato detoxicating method of breeding, it is characterized in that, the method is carried out according to the following steps:
The screening of matrix and sterilization: select that there is certain water conservation, fertilizer-preserving ability, the transplanting medium of easily sterilization, then to its thorough disinfection, to kill all pathogenic bacteria;
The cleaning of seedbed or seedling furrow and net canopy and sterilization: transplant first 2-3 days to net canopy space disinfection, kill various worm's ovum, and at laid inside cloth or plastic foil, be layered on described cloth or plastic foil after matrix sterilization;
Transplant: select optimum transplanting to transplant opportunity, and when detoxic seedling bottle outlet, the medium above it be cleaned up, and its root is carried out disinfection;
Detoxic seedling field planting about one month, cut seedling point production cuttage seeding, and carry out standardization:
(1) moisture controls: start to water to matrix permeable before and after transplanting, and the number of times after this surely watered according to the actual conditions of seedling every day and amount, control the wet poison of air by micro-spray or adopt plastics Small plastic shed to improve air wet malicious;
(2) illumination controls: will have sunshade condition, based on scattered light to new detoxic seedling of transplanting;
(3) temperature controls: detoxic seedling transplant after in 15-20 days, in net canopy and Small plastic shed, temperature should control within 30 degrees Celsius;
(4) Ventilation Control: detoxic seedling field planting is after 7-10 days, and start to ventilate according to the situation of taking root, ventilating opening is ascending, can remove plastic foil after 15-20 days;
(5) fertilizer controls: after detoxic seedling field planting 3-5 days start to water sour water once, and after taking root, available 1 times of MS solution irrigates, and along with the increase of seedling age, use the MS solution of about 1.5 times, and according to the growing way of detoxic seedling, two kinds of solution are used alternatingly, and in good time teeming calcium water;
Wherein said cut sharp size be two leaves wholeheartedly;
2. the potato detoxicating as claimed in claim 1 method of breeding, it is characterized in that, wherein said transplanting medium, for have good compacting covering effect to stolon, especially, is vermiculite;
3. the potato detoxicating as claimed in claim 1 method of breeding, it is characterized in that, wherein 1) the thorough disinfection method described in, i.e. 28-35 days before transplanting, water with formaldehyde 100 times of liquid of 38%-45% and drench matrix, then covering with plastic film 7-10 days in seedbed or seedling furrow, within after taking off film 10-15 days, transplant detoxic seedling, period digs matrix at least 1-2 time again, and formaldehyde odor is volatilized as early as possible;
4. the cloth the potato detoxicating as claimed in claim 1 method of breeding, is characterized in that, wherein 2) or plastic foil need sterilization and disinsection before use, and the stromal thickness on described cloth or plastic foil is 7-9 cm;
5. the potato detoxicating as claimed in claim 1 method of breeding, is characterized in that, wherein 2), net canopy space is carried out disinfection, use flolimat or phoxim baythion 1000 times of liquid;
6. the potato detoxicating as claimed in claim 1 method of breeding, it is characterized in that, wherein 3) transplanting described in is opportunity, after the expansion of potato virus-free plantlet subculture is numerous, in blake bottle, grow about 18-20 days, now height of seedling 6-8 centimetre, every young plant has root 5-8 bar, the color of the tip of a root is the vigorous yellow-white of cell division, and maximum leaf area is 0.45-0.50 square centimeter;
7. the root the potato detoxicating as claimed in claim 1 method of breeding, is characterized in that, wherein 3) carries out disinfection, and uses potassium permanganate dilution, root-inducing powder and bactericide mixed diluting liquid to do root and disinfects;
8. the potato detoxicating as claimed in claim 1 method of breeding, it is characterized in that, in described method, the whole growth cycle of detoxic seedling all will be prevented and treated damage by disease and insect, regularly, especially, within 7-10 days, carries out injection sterilization, insecticide; Especially, carbendazim 800, Sandofan 1000 times of liquid, Avermectin 1500 times of liquid, aphid difficult to understand 1500 times of liquid etc. can be used, be successively used alternatingly;
9. the potato detoxicating as claimed in claim 1 method of breeding, it is characterized in that, wherein 4) illumination described in controls, specifically refer to the sunshade net drawing 1-2 layer shading rate more than 60% outside net canopy, according to the recovery situation of seedling and survival rate after 10-15 days, progressively strengthen illumination, until seedling complete reform of nature environmental condition the dismountable sunshade measure in rear of looking sturdy.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856908A (en) * | 2016-12-26 | 2017-06-20 | 新昌县钧国生物技术有限公司 | High efficiency, low cost potato virus-free plantlet and original silkworm egg production technology |
| CN106962158A (en) * | 2017-03-27 | 2017-07-21 | 张掖市农业科学研究院 | A kind of potato expands numerous matrix and preparation method thereof |
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2014
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856908A (en) * | 2016-12-26 | 2017-06-20 | 新昌县钧国生物技术有限公司 | High efficiency, low cost potato virus-free plantlet and original silkworm egg production technology |
| CN106962158A (en) * | 2017-03-27 | 2017-07-21 | 张掖市农业科学研究院 | A kind of potato expands numerous matrix and preparation method thereof |
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