CN105481604A - Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof - Google Patents

Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof Download PDF

Info

Publication number
CN105481604A
CN105481604A CN201510890997.7A CN201510890997A CN105481604A CN 105481604 A CN105481604 A CN 105481604A CN 201510890997 A CN201510890997 A CN 201510890997A CN 105481604 A CN105481604 A CN 105481604A
Authority
CN
China
Prior art keywords
parts
liquid fertilizer
compound
biological organic
organic liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510890997.7A
Other languages
Chinese (zh)
Inventor
程玉山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HINON BIOTECHNOLOGY
Original Assignee
HINON BIOTECHNOLOGY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HINON BIOTECHNOLOGY filed Critical HINON BIOTECHNOLOGY
Priority to CN201510890997.7A priority Critical patent/CN105481604A/en
Publication of CN105481604A publication Critical patent/CN105481604A/en
Pending legal-status Critical Current

Links

Landscapes

  • Fertilizers (AREA)

Abstract

The invention provides a compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer, which comprises main raw materials of seaweed, kelp, Ganoderma applanatum, Coprinus comatus, pollen, shoestring fungus, Gynostemma pentaphylla, spirulina, Hericium erinaceus, cicada fungus, saffron, Isaria cicadae Miquel, Ganoderma lucidum, and seaweed dietary fiber. The present invention also provides a preparation method of the organic liquid fertilizer and application thereof. The liquid fertilizer can effectively improve the crop root growth environment, promote the root growth, facilitate the absorption of mineral nutrition in soil by the roots, prevent aging of crops, improve yield and quality of the crops, and enhance the disease resistance of crops. For the aspect of poultry feed, due to the role of photosynthetic bacteria, the liquid fertilizer can eliminate odor of feces, and purify the sludge, wastewater and waste residues in pigpen, cattle pen and sheep pen, so that the liquid fertilizer produced from farming can reach the use standards, improve the nutritional effect of liquid fertilizer on plant, and prevent influenza in poultry.

Description

Biological organic liquid fertilizer of the active beneficial mother bacterial liquid of a kind of compound EM and its preparation method and application
Technical field
The invention belongs to microbial liquid bacterial manure field, particularly relate to biological organic liquid fertilizer of the active beneficial mother bacterial liquid of a kind of compound EM and its preparation method and application.
Background technology
In recent years, along with the raising of agricultural productive force, domestic microbiological fertilizer market is also more and more active, many microbial fertilizers come into the market, wherein with fixing nitrogen, dissolving phosphor and dissolving potassium, single bacterium multifunctional type most representativeness, the development experience of China's microbial preparation nitragin, bacterial fertilizer (bacterial manure) are to the transition of microbial fertilizer, dressed seed by pulse family Inoculant, bacterial classification and develop into the base manure of various farm crop, some microorganisms, owing to producing active substance, are also used as foliar applied fertilizer sometimes.China's microbial fertilizer research and development and Applicative time comparatively western developed country, starting is late, and product development is in the starting stage, and effect and stability are all difficult to the requirement reaching large scale application.
At present, there is genetically engineered bacterial manure both at home and abroad, be used as base manure and the organo-mineral complexing bacterial manure topdressed, the microbial fertilizer of biological organic fertilizer, non-grass high-area carbon highdensity bacterium powder type microbial inoculant agent fertilizer and other several functions types and title.In future, organic fertilizer decomposing microbial inoculum, soil remediation microbial inoculum, nitragin and phosphorus-solubilizing bacteria agent and the special product such as function microbial inoculum, biological organic fertilizer, drought resisting microbial inoculum of the cash crop such as fruit tree, vegetables, will become emphasis and focus that research and development promote.Along with the development of biotechnology and the infiltration of new and high technology, microbial-bacterial fertilizer has good production application prospect.Fertilizer must coordinate bacterial manure to use could to accelerate decomposition organic in fertilizer, is more conducive to Crop, reduces nutrient loss.Visible microbial-bacterial fertilizer has started to play a role in agriculture production, achieves certain economic benefit and social benefit, tentatively enters the regular suitability for industrialized production stage.But statistic data display, western countries' organic fertilizer consumptions such as the current U.S., Japan, Britain have accounted for 50 parts of Fertilizer application total amount, but China's organic fertilizer usage quantity accounting is less than 10 parts; For market scale, within 2013, China's Production of manure industrial market scale breaks through 9,000 hundred million yuan, and organic fertilizer market scale is less than 80,000,000,000 yuan.
Microorganism fertilizer mixes mainly with a kind of, tens kinds or tens kinds of microbial bacterias in the world, extract bacterial strain rank relatively low, its effect is single, limited use, product has peculiar smell simultaneously, a lot of product stock also needs cryopreservation, a lot of product in use complex operation, time-consuming, bring many restrictions aborning.Therefore, market being badly in need of one can effectively overcome the above problems, and effective microbe bacterium is many, can provide fast, convenient, effective green product for agriculture production.
Summary of the invention
Single in order to solve microorganism fertilizer microbial bacteria in prior art, extract bacterial strain rank relatively low, its effect is single, limited use, product has peculiar smell simultaneously, in use complex operation, time-consuming, bring the problem of many restrictions aborning, the invention provides a kind of effective microbe bacterial classification many, the agricultural output because a large amount of use chemical fertilizer and agricultural chemicals cause and quality decline can be solved, barrier of the soil, nutrient loss and the air caused, soil, the problem that water etc. pollute, can effectively improve crop root growth environment again, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of the compound EM promoting root system to produce.
The present invention is achieved through the following technical solutions:
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of a kind of compound EM, described component, with parts by weight, comprising: marine alga 4-8 part, kelp (sea-tangle) 7-11 part, cypress bacterium 7-11 part, Coprinus comatus 3-5 part, pollen 5-7 part, armillaria mellea 5-7 part, the blue 6-10 part of glue stock, spirulina 10-14 part, Hericium erinaceus (Bull. Ex Fr.) Pers. 1-3 part, cicada fungus 2-4 part, style and stigma of Saffron Crocus 8-12 part, hat cicada 5-9 part, local tyrant's Ganoderma 12-16 part, seaweed diet fiber 2-6 part.
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM described above, comprising: marine alga 6 parts, kelp 9 parts, cypress bacterium 9 parts, Coprinus comatus 4 parts, pollen 6 parts, armillaria mellea 6 parts, blue 8 parts of glue stock, spirulina 12 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 2 parts, cicada fungus 3 parts, style and stigma of Saffron Crocus 10 parts, hat cicada 7 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts.
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM described above, contains: marine alga 4 parts, kelp 11 parts, cypress bacterium 8 parts, Coprinus comatus 5 parts, pollen 5 parts, armillaria mellea 7 parts, blue 7 parts of glue stock, spirulina 13 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 3 parts, cicada fungus 2 parts, style and stigma of Saffron Crocus 9 parts, hat cicada 8 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts.
Present invention also offers the preparation method of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of a kind of compound EM, comprise the following steps:
Get the raw materials ready: with parts by weight, take marine alga, kelp, cypress bacterium, Coprinus comatus, pollen, armillaria mellea, glue stock orchid, spirulina, Hericium erinaceus (Bull. Ex Fr.) Pers., cicada fungus, style and stigma of Saffron Crocus, hat cicada, local tyrant's Ganoderma, seaweed diet fiber main raw material, for subsequent use;
By raw material mixing and stirring, filter after low temperature 60-70 DEG C of boiling 2-4h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
Present invention also offers the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of a kind of described compound EM in increasing crop yield, poultry cultivation, the application improved in ecological environment of soil.
Marine alga of the present invention, kelp, cypress bacterium, Coprinus comatus, pollen, armillaria mellea, glue stock orchid, spirulina, Hericium erinaceus (Bull. Ex Fr.) Pers., cicada fungus, style and stigma of Saffron Crocus, hat cicada, local tyrant's Ganoderma, seaweed diet fiber, be commercially available prod.
Beneficial effect of the present invention is mainly reflected in the following aspects:
(1) compared with prior art, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM of the present invention, as novel, highly enriched, high efficiency microbiological composite bacterial agent, there is obvious volume increase, synergy and resistance against diseases, to farm crop and agroecological environment, there is active effect, be conducive to the use reducing chemical fertilizer.
(2) raw material sources of the present invention are extensive, and formula is simple, and preparation method is simple, applies in the diet of described crop soil or described poultry during application, easy to use.
(3) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM of the present invention, has obvious fixed nitrogen, phosphorus decomposing and potassium decomposing, strengthens beneficial microbe colony, improve ecological environment of soil, strengthen the effect of improving activity of root system.
(4) use the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM of the present invention, be conducive to environment purification, the ight soil produce poultry farming and waste water have obvious cleaning action, are conducive to environment purification.
(5) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM of the present invention, has obvious promoter action to plant husbandry and aquaculture, has very high economic benefit and Ecological Society effect.
(6) reasonable ratio: by the kind in screening prescription and concrete proportioning, obtain beneficial microbe colony the most rationally, the strongest liquid organic fertilizer of effect, each composition play each other complement each other, the technique effect of synergy.
Embodiment
Below in conjunction with embodiment, further illustrate content of the present invention.Should be appreciated that enforcement of the present invention is not limited to the following examples, any pro forma accommodation make the present invention and/or change all will fall into scope.
In the present invention, if not refer in particular to, all parts, per-cent are weight unit, and all equipment and raw material etc. all can be buied from market or the industry is conventional.Method in following embodiment, if no special instructions, is the ordinary method of this area.
Embodiment 1
According to the biological organic liquid fertilizer formula of the active beneficial mother bacterial liquid of compound EM, claim marine alga 6kg, kelp 9kg, cypress bacterium 9kg, Coprinus comatus 4kg, pollen 6kg, armillaria mellea 6kg, glue stock blue 8kg, spirulina 12kg, Hericium erinaceus (Bull. Ex Fr.) Pers. 2kg, cicada fungus 3kg, style and stigma of Saffron Crocus 10kg, hat cicada 7kg, local tyrant's Ganoderma 14kg, seaweed diet fiber 4kg.Raw material is stirred well to and mixes, filter after low temperature 60 DEG C of boiling 2h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
Embodiment 2
According to the biological organic liquid fertilizer formula of the active beneficial mother bacterial liquid of compound EM, take marine alga 4kg, kelp 11kg, cypress bacterium 8kg, Coprinus comatus 5kg, pollen 5kg, armillaria mellea 7kg, glue stock blue 7kg, spirulina 13kg, Hericium erinaceus (Bull. Ex Fr.) Pers. 3kg, cicada fungus 2kg, style and stigma of Saffron Crocus 9kg, hat cicada 8kg, local tyrant's Ganoderma 14kg, seaweed diet fiber 4kg.By raw material mixing and stirring, filter after low temperature 70 DEG C of boiling 3.5h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
Embodiment 3
According to the biological organic liquid fertilizer formula of the active beneficial mother bacterial liquid of compound EM, take marine alga 8kg, kelp 7kg, cypress bacterium 11kg, Coprinus comatus 3kg, pollen 5kg, armillaria mellea 7kg, glue stock blue 9kg, spirulina 11kg, Hericium erinaceus (Bull. Ex Fr.) Pers. 3kg, cicada fungus 2kg, style and stigma of Saffron Crocus 8kg, hat cicada 9kg, local tyrant's Ganoderma 14kg, seaweed diet fiber 4kg.By raw material mixing and stirring, filter after low temperature 65 DEG C of boiling 4h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
The result of use of experimental example 1 pig farm cultivation
Pig farm cultivation faces much production and utilizes the problem of aspect, first liable to illness the and communicate illness mutually of pig, and the easy contaminate environment of the waste residue such as ight soil and urine, waste water, harmless treatment is improper easily causes air, environment and water pollution.The use of microorganism liquid fertilizer of the present invention effectively can avoid the problems referred to above, for clean utilization and solution environmental pollution provide effective way.
Using method:
The biological organic liquid fertilizer of active for compound EM in embodiment 1 beneficial mother bacterial liquid is added in tap water by porkling every 50ml every day; The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM in 100ml-200ml embodiment 1 is sprayed by every 100 square metres above pigsty; In pigsty purification tank, add the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM in embodiment 1, form liquid fertilizer.
Porkling feeds the impact that the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is grown up on porkling:
The biological organic liquid fertilizer product of the active beneficial mother bacterial liquid of compound EM in embodiment 1 is fed to porkling, the hair color of pig is shinny, plump and sturdy, mean body weight increases by more than 10%, contain than yeast in this and the photosynthetic bacterium in the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM in embodiment 1, the protein content of the high price such as soybean feed is higher, and various amino acid ratio more balances, digestibility is higher relevant, contain abundant carotene in photosynthetic bacterium simultaneously, various vitamin b6 usp (B2, B6, B12, D, E) particularly B12, facilitate the bone growth of pig, be conducive to it to grow, anti-stress, cold-resistant, anti-fatty liveranti-fatty liver and preventing cancer.
Pigsty sprays the impact of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM in embodiment 1 on porkling diseases prevention and living environment:
By the effect to the biological organic liquid fertilizer product of the active beneficial mother bacterial liquid of compound EM in pigsty uppermost spray embodiment 1, stench in circle obviously alleviates, some circles are improved substantially, this is relevant containing photosynthetic bacterium with the biological organic liquid fertilizer product of the active beneficial mother bacterial liquid of compound EM, because containing the performance decomposing odor pollutant in photosynthetic bacterium; From contrast, spraying the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM effectively can prevent cold pig, and this is mainly relevant containing antiviral substance with photosynthetic bacterium.
Pig manure after the biological organic liquid fertilizer purification of the active beneficial mother bacterial liquid of compound EM and liquid fertilizer are on the impact of vegetable growth:
(1) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is on the impact of radish percentage of germination and root system development
One, materials and methods
Select sieve of the same size to foretell to plant, after cleaning with clear water, after urinating 10 times of diluents and the biological organic liquid fertilizer process of the active beneficial mother bacterial liquid of pig urine interpolation compound EM with irrigation water, pig respectively, 10 times of diluents are soaked seed 3 hours to radish, put into the culture dish with gauze more respectively, 100, each culture dish, put into 25 DEG C of illumination box light culture, keep moisture with respective handling solution respectively, after 5 days, survey percentage of germination.Root length is surveyed after 8 days.
Two, irrigation water, pig urine and process water germinate and the impact of root system development on radish
Table 1 result shows, relative to radish percentage of germination, and the percentage of germination that irrigation water and the pig urine process water two after the biological organic liquid fertilizer process of the active beneficial mother bacterial liquid of compound EM process all 100%, and undressed pig urine percentage of germination is only 81%.Root growth aspect, compared with contrast (irrigation water), the process of pig urine obviously affects root growth, and taproot and root total length reduce by 45.7% and 56.0% respectively; Process water after the biological organic liquid fertilizer process of the active beneficial mother bacterial liquid of compound EM then obviously promotes the growth of radish root system, and taproot and total root length increase by 11.4% and 11.4% respectively.
Draw from the above results, the dilution of stock of pig urine obviously hinders the germination of seed and the growth of root system; After adding the biological organic liquid fertilizer product of the active beneficial mother bacterial liquid of compound EM, adding of photosynthetic bacterium does not only hinder the germination of seed and the growth of root system, when not affecting seed germination, significantly facilitate the growth of root system especially, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of visible compound EM obviously facilitates the vigor of root system.
Table 1 irrigation water, pig are urinated and process water germinates on radish and the impact of root system development
(2) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is on the impact of vegetable growth
One, materials and methods
Spinach is planted in booth, take chemical fertilizer as contrast, different B OD value after the biological organic liquid fertilizer process of the active beneficial mother bacterial liquid of pig urine compound EM is different treatment, 4 process are established in test, often process application quantity of nitrogen phosphorus potassium consistent, wherein different B OD value is the aerobic pollution content such as the organism of the process water of pig urine after the process of compound EM active beneficial mother bacterial liquid biology organic liquid fertilizer
Two, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is on the impact of spinach growth
Draw from table 2, same application quantity of nitrogen phosphorus potassium, use the process cauline leaf of chemical fertilizer, root and complete stool fresh weight all minimum, what organic big porker urinated uses the content significantly improving spinach cauline leaf, root and complete stool fresh weight, improve 15.1 times, 19.9 times and 16.0 times respectively, wherein along with the minimizing of BOD value, cauline leaf, root and complete stool fresh weight are all multiplied, the increase of the biological organic liquid fertilizer consumption of the active beneficial mother bacterial liquid of compound EM is described, pig urine BOD value obviously reduces, and is the principal element improving spinach output.
After the active beneficial mother bacterial liquid of table 2 compound EM biological organic liquid fertilizer process pig urine, different B OD value is on the impact of spinach growth
In sum, the biological organic liquid fertilizer effect of the active beneficial mother bacterial liquid of compound EM of the present invention is summed up:
1) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM promotes the growth of porkling, and preventing disease is particularly particularly evident to flu preventive and therapeutic effect simultaneously;
2) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM effectively can reduce the BOD value of pig manure urine, has obvious cleaning action, effectively can remove stench, activation nutrient, improve utilising efficiency;
3) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM can eliminate the impact that the ammonia of pig urine and hydrogen sulfide germinate on radish, obviously promotion radish root growth.
The utilizing status in experimental example 2 poulty house
Egg is rich in cholesterol, nutritious, and its proteinaceous amino acid ratio meets Human Physiology needs, and utilization ratio, up to more than 98%, is one of daily edible food of the mankind.The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM effectively can shift to an earlier date laying period and improve laying rate, and egg quality is improved significantly.
Using method:
Feed: laying hen tap water adds the biological organic liquid fertilizer (reaching 0.01-0.02% for standard with the photosynthetic bacterium in tap water) of the active beneficial mother bacterial liquid of 10-20 milliliter compound EM by every 100 premium on currency, drinks 30 days;
Spray: every 100 square meter hen houses, with being sprayed at after the dilution of the active beneficial mother bacterial liquid of compound EM biological organic liquid fertilizer 100-200 milliliter around on ground and wall.
Chicken feeds the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM to the impact of growth and development of chickens:
By feeding the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM to chicken, first the feed intake of chicken significantly improves.Yolk that chicken lays eggs increases, and yolk index reaches 47-49%; Ovum blue-stain obtains stocky, and the blue or green coefficient of ovum reaches 80-84.5; The content of the carotene in yolk and Vitamin A increases by 20%, and cholesterol level reduces.In the whole spawning time, lay eggs about 7 days in advance, the whole spawning time extends 2-3 month, and spawning rate increases by more than 10%, and rate of broken eggs reduces.The raising of all these egg yield and qualities, may with photosynthetic bacterium in the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM in containing higher than yeast, the content of the high price such as soybean feed protein, wherein various amino acid ratio compares balance, digestibility is higher relevant, for promotion red blood cell development and maturation, prevention pernicious anemia, safeguard that neural system is healthy, promote carbohydrate, the vitamin B12 of fat and protein metabolism, photosynthetic bacterium provides in the biological organic liquid fertilizer product of the active beneficial mother bacterial liquid of compound EM just, the active effect of visible microbial-bacterial fertilizer in laying hen nursing.
Feed and spray the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, in chicken house, stench alleviates, and the virus of chicken incidence of particularly catching a cold obviously reduces, and the performance that this and photosynthetic bacterium in product contain antiviral substance and peculiar decomposition odor pollutant is relevant.
In sum, the biological organic liquid fertilizer effect of the active beneficial mother bacterial liquid of compound EM of the present invention is summed up:
1) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM can significantly improve laying rate of chicken more than 12%, and rate of broken eggs reduces, and in yolk, the content of carotene and Vitamin A increases by 23% simultaneously, reduces cholesterol level;
2) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM obviously can strengthen the ability of chicken resist the disease, can effective preventing cold;
3) by spraying the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, can obviously alleviate chicken house stench, can effectively purify chicken house environment.
Experimental example 3 is result of use on tomato and eggplant
Materials and methods:
Test arrangement carries out in booth.At annual every 666.7m 2use cow dung 20m 3basis is arranged ck (contrast, water), water and execute the micro-fertilizer of the compound EM biological organic liquid fertilizer of active beneficial mother bacterial liquid (the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of 10kg compound EM dilutes 1000 times and waters at twice and execute, be separated by for twice 15 days, before wherein first time is arranged in field planting) two test process, often process and repeat for 3 times, every community 133.3m 2.Soil is Shajiang black soil.
The impact that the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM grows on tomato and eggplant:
Table 3 test-results shows, and adopts root to water and executes the biological organic liquid fertilizer microbial-bacterial fertilizer of the active beneficial mother bacterial liquid of compound EM, can significantly improve the output of tomato and eggplant, improve 21.3% and 9.3% respectively; From photosynthesis, the use of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, significantly improves the photosynthesis of tomato and eggplant equally, respectively than contrast raising 40.1% and 34.9%.Visible, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is significantly improving tomato and eggplant is photosynthetic while, also the output of tomato and eggplant is significantly improved, this and the biological organic liquid fertilizer of the beneficial mother bacterial liquid of compound EM activity promote nutrients release in cow dung and soil, make the nutrient that vegetables energy is timely, balanced, abundance obtains needed for growth relevant.
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of table 3 compound EM is to tomato, eggplant yield (kg/666.7m 2) and photosynthesis (umol.m -2.s -1) impact
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is on the impact of tomato and eggplant quality and disease resistance:
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM significantly improves the Vc content of tomato and eggplant, improves 10.7% and 5.8% respectively; Soluble solid aspect improves 21.9% and 13.3% respectively; Nitrate content also significantly improves 5.2% and 14.0%; Soluble sugar impact then not obvious (see table 4).Disease resistance aspect, do not execute the tomato process (ck contrasts) of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM the diseased plant of late blight reaches 36 strains (by every 666.7m 2note); and the process tomato of using the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM does not obtain late blight; tomato late blight is one of important disease on tomato; protecting field, open country all can occur; the time harm that continuous rainy weather is many is serious, causes that stem rots, plant wither and fruit browning look when falling ill serious, has a strong impact on output; visible, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM can significantly improve the ability of the anti-late blight of tomato.The results are shown in Table 4.
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of table 4 compound EM is on the impact of tomato and eggplant quality and disease resistance
In sum, the biological organic liquid fertilizer effect of the active beneficial mother bacterial liquid of compound EM of the present invention is summed up:
1) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM significantly improves tomato and eggplant yield, improves 21.3% and 9.3% respectively;
2) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM significantly improves the quality of tomato and eggplant, and Vc and soluble solid obviously increase;
3) nitrate content increase shows that the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM obviously promotes the release that cow dung and Nitrogen In Soils attainment are divided, promote to absorb crop nitrogen, thering is provided foundation for reducing chemical nitrogen fertilizer use, being conducive to the pollution avoiding the excessive use of nitrogenous fertilizer to bring;
4) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is conducive to the disease resistance improving tomato, particularly enhances the resistance against diseases of tomato to late blight.
The result of use of experimental example 4 on horticulture and flower
Owing to watering water excess, overfrequency, basin soil is long-term excessively wet, soil anoxic, and the respiration of root is obstructed, and physiological function weakens, and resistibility declines, anerobe wantonly act under the environment of anoxic, produces obnoxious flavour, poisons root system; Flower of Arabian Jasmine is happiness acid soil plant, can adapt to acid soil for a long time, and water flowers mainly with tap water, river, well water in the north, the salt and alkali component in water, causes jasmine growing way weak, and branches and leaves turn to be yellow; Long-term not soil removal and replacement, frequently waters or drenches with rain, and nutrient loss less than supplementing, defines again " starving yellow ".Above-mentioned some be potted Arabian jasmine flower yellow leaf or plant main causes of death, utilize the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM by carrying out fillings root test yellow leaf, the frequently Flower of Arabian Jasmine that dies on one's deathbed, to judge its impact on diseased plant Flower of Arabian Jasmine vital movement.
Materials and methods:
The diseased plant Flower of Arabian Jasmine (plant height 35 centimetres) select yellow leaf, frequently dying on one's deathbed, fill with root 3 times with the biological organic liquid fertilizer microbial inoculum of the active beneficial mother bacterial liquid of 1000 times of compound EM by 150 milliliters points, every basin, management of watering is by daily management.Measure its photosynthetic change by the photosynthetic determinator of CIRAS-1, in the change of auxiliary visual inspection diseased plant jasmine, and the change of leaf growth judges that the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is on the impact of Flower of Arabian Jasmine.The results are shown in Table 5.
Use the impact that the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM grows diseased plant Flower of Arabian Jasmine:
By using the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM to diseased plant Flower of Arabian Jasmine, within 7 days, there is obvious change with rear blade, blade by yellow, turn green gradually, sprouting starts to become green, the green intensification further of rear blade in 15 days, within 30 days, rear blade recovers normal, leaf look dark green, grows vigorous, and blade obviously increases than original blade, visible plant vital activity strengthens, improve root system ecotope, activating soil nutrient with the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, promote that the absorption of root system to nutrient is relevant.
The biological organic liquid fertilizer of the active beneficial mother bacterial liquid of table 5 compound EM is on the impact of Flower of Arabian Jasmine leaf photosynthesis and leaf area
In sum, the biological organic liquid fertilizer effect of the active beneficial mother bacterial liquid of compound EM of the present invention is summed up:
1) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM is by root system effect, obviously promotes the vital movement of Flower of Arabian Jasmine plant, and be conducive to leaf growth and grow, blade area increases about 1 times;
2) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM promotes Flower of Arabian Jasmine plant leaf photosynthesis, and photosynthetic raising, more than 2 times, provides the matter and energy of vital movement.
3) the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM makes the Flower of Arabian Jasmine frequently died on one's deathbed be lived again.
Although contriver has done comparatively detailed elaboration to technical scheme of the present invention and has enumerated; be to be understood that; for one, this area those skilled in the art; amendment is made to above-described embodiment or adopts equivalent replacement scheme; this is apparent to those skilled in the art; these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.

Claims (9)

1. the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, it is characterized in that: with parts by weight, comprise following component: marine alga 4-8 part, kelp 7-11 part, cypress bacterium 7-11 part, Coprinus comatus 3-5 part, pollen 5-7 part, armillaria mellea 5-7 part, the blue 6-10 part of glue stock, spirulina 10-14 part, Hericium erinaceus (Bull. Ex Fr.) Pers. 1-3 part, cicada fungus 2-4 part, style and stigma of Saffron Crocus 8-12 part, hat cicada 5-9 part, local tyrant's Ganoderma 12-16 part, seaweed diet fiber 2-6 part.
2. the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 1, it is characterized in that, comprising: marine alga 6 parts, kelp 9 parts, cypress bacterium 9 parts, Coprinus comatus 4 parts, pollen 6 parts, armillaria mellea 6 parts, blue 8 parts of glue stock, spirulina 12 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 2 parts, cicada fungus 3 parts, style and stigma of Saffron Crocus 10 parts, hat cicada 7 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts.
3. the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 1, it is characterized in that, contain: marine alga 4 parts, kelp 11 parts, cypress bacterium 8 parts, Coprinus comatus 5 parts, pollen 5 parts, armillaria mellea 7 parts, blue 7 parts of glue stock, spirulina 13 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 3 parts, cicada fungus 2 parts, style and stigma of Saffron Crocus 9 parts, hat cicada 8 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts.
4. a preparation method for the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM, is characterized in that, comprise the following steps:
Get the raw materials ready: with parts by weight, take marine alga 4-8 part, kelp 7-11 part, cypress bacterium 7-11 part, Coprinus comatus 3-5 part, pollen 5-7 part, armillaria mellea 5-7 part, the blue 6-10 part of glue stock, spirulina 10-14 part, Hericium erinaceus (Bull. Ex Fr.) Pers. 1-3 part, cicada fungus 2-4 part, style and stigma of Saffron Crocus 8-12 part, hat cicada 5-9 part, local tyrant's Ganoderma 12-16 part, seaweed diet fiber 2-6 part, for subsequent use;
Filter after above-mentioned raw materials is fully mixed rear low temperature boiling, concentrate to obtain the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM.
5. the preparation method of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 4, is characterized in that, by raw material mixing and stirring, filter after 60-70 DEG C of boiling 2-4h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
6. the preparation method of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 5, is characterized in that,
Take marine alga 6 parts, kelp 9 parts, cypress bacterium 9 parts, Coprinus comatus 4 parts, pollen 6 parts, armillaria mellea 6 parts, blue 8 parts of glue stock, spirulina 12 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 2 parts, cicada fungus 3 parts of, Xi Red spend 10 parts, hat cicada 7 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts;
By above-mentioned raw materials mixing and stirring, filter after 60 DEG C of boiling 2h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
7. the preparation method of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 5, is characterized in that,
Take marine alga 4 parts, kelp 11 parts, cypress bacterium 8 parts, Coprinus comatus 5 parts, pollen 5 parts, armillaria mellea 7 parts, blue 7 parts of glue stock, spirulina 13 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 3 parts, cicada fungus 2 parts of, Xi Red spend 9 parts, hat cicada 8 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts;
By above-mentioned raw materials mixing and stirring, filter after 70 DEG C of boiling 3.5h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
8. the preparation method of the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of compound EM according to claim 5, is characterized in that,
Take marine alga 8 parts, kelp 7 parts, cypress bacterium 11 parts, Coprinus comatus 3 parts, pollen 5 parts, armillaria mellea 7 parts, blue 9 parts of glue stock, spirulina 11 parts, Hericium erinaceus (Bull. Ex Fr.) Pers. 3 parts, cicada fungus 2 parts of, Xi Red spend 8 parts, hat cicada 9 parts, local tyrant's Ganoderma 14 parts, seaweed diet fiber 4 parts;
By above-mentioned raw materials mixing and stirring, filter after 65 DEG C of boiling 4h, concentrated, the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of obtained compound EM.
9. the biological organic liquid fertilizer of the active beneficial mother bacterial liquid of the compound EM that one of claim 1-3 is described is in increasing crop yield, poultry cultivation, the application improved in ecological environment of soil.
CN201510890997.7A 2015-10-16 2015-12-04 Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof Pending CN105481604A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510890997.7A CN105481604A (en) 2015-10-16 2015-12-04 Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2015106772058 2015-10-16
CN201510677205 2015-10-16
CN201510890997.7A CN105481604A (en) 2015-10-16 2015-12-04 Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN105481604A true CN105481604A (en) 2016-04-13

Family

ID=55668929

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510890997.7A Pending CN105481604A (en) 2015-10-16 2015-12-04 Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN105481604A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105850466A (en) * 2016-04-28 2016-08-17 平顶山市绿禾农业科技开发有限公司 Soybean planting method capable of increasing yield through plant hormone
CN106068971A (en) * 2016-05-26 2016-11-09 温立刚 Special efficacy Herba Rosmarini Officinalis cultural method
CN111052985A (en) * 2019-12-31 2020-04-24 北京大道东圣生物科技有限公司 Myxomycete hypha biological extract and preparation method and application thereof
CN114747444A (en) * 2022-04-29 2022-07-15 四川农业大学 Cultivation method for improving radish quality

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1463950A (en) * 2002-06-06 2003-12-31 北京绿源晨光生物工程技术开发有限公司 Nuisance free biological organic fertilizer and method for preparing same
JP2004075470A (en) * 2002-08-20 2004-03-11 Hideko Yamaguchi Thermoplastic organic composition
CN101041601A (en) * 2007-04-30 2007-09-26 山东省农业科学院土壤肥料研究所 Foliage fertilizer containing glossy ganoderma leach and preparation method thereof
CN103859215A (en) * 2014-03-11 2014-06-18 山东新希望六和集团有限公司 Feed for laying hens in egg producing period and preparation method of feed
CN104068243A (en) * 2014-05-13 2014-10-01 范治淮 Ecological crucian carp feed and preparation method thereof
CN104187038A (en) * 2014-07-18 2014-12-10 张守进 Easily digestible piglet feed and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1463950A (en) * 2002-06-06 2003-12-31 北京绿源晨光生物工程技术开发有限公司 Nuisance free biological organic fertilizer and method for preparing same
JP2004075470A (en) * 2002-08-20 2004-03-11 Hideko Yamaguchi Thermoplastic organic composition
CN101041601A (en) * 2007-04-30 2007-09-26 山东省农业科学院土壤肥料研究所 Foliage fertilizer containing glossy ganoderma leach and preparation method thereof
CN103859215A (en) * 2014-03-11 2014-06-18 山东新希望六和集团有限公司 Feed for laying hens in egg producing period and preparation method of feed
CN104068243A (en) * 2014-05-13 2014-10-01 范治淮 Ecological crucian carp feed and preparation method thereof
CN104187038A (en) * 2014-07-18 2014-12-10 张守进 Easily digestible piglet feed and preparation method thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105850466A (en) * 2016-04-28 2016-08-17 平顶山市绿禾农业科技开发有限公司 Soybean planting method capable of increasing yield through plant hormone
CN106068971A (en) * 2016-05-26 2016-11-09 温立刚 Special efficacy Herba Rosmarini Officinalis cultural method
CN111052985A (en) * 2019-12-31 2020-04-24 北京大道东圣生物科技有限公司 Myxomycete hypha biological extract and preparation method and application thereof
CN114747444A (en) * 2022-04-29 2022-07-15 四川农业大学 Cultivation method for improving radish quality

Similar Documents

Publication Publication Date Title
CN101225007B (en) Special biological fish-fertilizer for aquaculture and preparation method thereof
CN107098784A (en) Biological organic fertilizer and preparation method thereof
US9150461B2 (en) Bioorganic agent for treating plants (variants)
CN102150538B (en) Selenium-enriched paddy seedling and cultivation method thereof and produced organic selenium-enriched rice
CN103392642B (en) Method for breeding mandarin fish by utilizing microorganic feed
CN102696458A (en) Edible fungus compost, production method thereof and edible fungus culture process
CN105085120A (en) Special organic fertilizer suitable for tea tree planting
CN104844313A (en) Method for preparing dedicated bio-organic fertilizer for selenium-enriched cherry tomatoes
CN102150537A (en) Selenium-rich vegetable seedlings, cultivation method thereof and vegetables produced rich in organic selenium
CN102584393A (en) Special organic and inorganic compound fertilizer for loquat tree and preparation method for special organic and inorganic compound fertilizer
CN104788237B (en) A kind of wheat selenium-rich zinc-iron microbial fermentation fertilizer and its preparation method and application
CN103120137A (en) Method of cultivating silver carps and bighead carps with organic fertilizer coordinated with biological fish fertilizer
CN105638302A (en) Nutrient soil for organic camellia oleifera culture
CN105481604A (en) Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof
CN109293438A (en) A kind of flower nutrient solution containing marine oligosaccharide
CN101935244B (en) Salt fertilization of water hyacinth fermentation liquor and application thereof
CN104609912A (en) A composite biological bacterial fertilizer based on a novel composite biological live bacterial preparation and a preparing method thereof
CN102150527A (en) Selenium-enriched herbal fruit seedling, breeding method thereof, and produced organic selenium-enriched fruit
CN102150528A (en) Se-enriched tea seedling, culturing method thereof and organic Se-enriched tea product
CN109845593A (en) A method of Chinese little greens quality is improved using earthworm and Chinese little greens mixing breeding
CN103355234B (en) A kind of method adopting microbial-bacterial fertilizer Isolated from Diseased Scophthalmus maximus
CN111226742B (en) Rice seedling raising substrate and preparation method thereof
CN105104041A (en) Production method of selenium-enriched rice
CN106748155A (en) A kind of organic mineral substance multi-element compound fertilizer and preparation method thereof
CN111470897A (en) Selenium-rich high-calcium rice foliar fertilizer and preparation method and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20160413

RJ01 Rejection of invention patent application after publication