CN114747444A - Cultivation method for improving radish quality - Google Patents
Cultivation method for improving radish quality Download PDFInfo
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- CN114747444A CN114747444A CN202210474471.0A CN202210474471A CN114747444A CN 114747444 A CN114747444 A CN 114747444A CN 202210474471 A CN202210474471 A CN 202210474471A CN 114747444 A CN114747444 A CN 114747444A
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- Prior art keywords
- shepherd
- purse
- radish
- cultivation method
- fermentation
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- 241000220259 Raphanus Species 0.000 title claims abstract description 37
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 title claims abstract description 37
- 238000012364 cultivation method Methods 0.000 title claims abstract description 12
- 235000011305 Capsella bursa pastoris Nutrition 0.000 claims abstract description 24
- 240000008867 Capsella bursa-pastoris Species 0.000 claims abstract description 24
- 238000000855 fermentation Methods 0.000 claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 8
- 239000000758 substrate Substances 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 239000003337 fertilizer Substances 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 9
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 10
- 229910052793 cadmium Inorganic materials 0.000 abstract description 8
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract description 5
- 229930003268 Vitamin C Natural products 0.000 abstract description 5
- 239000000835 fiber Substances 0.000 abstract description 5
- 235000019154 vitamin C Nutrition 0.000 abstract description 5
- 239000011718 vitamin C Substances 0.000 abstract description 5
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 abstract description 4
- 239000002028 Biomass Substances 0.000 abstract description 3
- 238000009825 accumulation Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- YKYOUMDCQGMQQO-UHFFFAOYSA-L cadmium dichloride Chemical compound Cl[Cd]Cl YKYOUMDCQGMQQO-UHFFFAOYSA-L 0.000 description 2
- 229940118019 malondialdehyde Drugs 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
Abstract
The invention provides a cultivation method for improving radish quality, which comprises the following steps: when two true leaves of radish seedlings are unfolded, applying shepherd's purse leavening to roots; the shepherd's purse leavening is obtained by fermenting shepherd's purse whole plant serving as a fermentation substrate and aspergillus niger A46CGMCC No.3084 serving as zymophyte. The invention can well improve the biomass and the root weight of the radish; the crude fiber content can be obviously reduced, the protein content can be obviously improved, the malonaldehyde content can be obviously reduced, and the vitamin C content can be improved; the invention can also obviously reduce the accumulation of cadmium in the radish.
Description
Technical Field
The invention belongs to a crop cultivation technology, and relates to a cultivation method for improving the quality of radishes.
Background
Radish is 1-year or 2-year herbaceous plant of Brassicaceae, and is a vegetable crop with edible, processing and medicinal values. Radish can enhance the body immunity, remove heat toxin in vivo, promote qi circulation and promote digestion, and has edible and medicinal values because of containing mineral substances, amino acids, fructose, crude fiber, glucose, vitamin C, sucrose and a small amount of crude protein.
The growth quality indexes of radish include protein, vitamin C, organic acid, crude fiber, soluble sugar, malondialdehyde content and the like, and researchers are concerned about the content of relevant heavy metals (such as cadmium) in radish. A common means for improving the growth quality of radishes is a fertilization technology, for example, Chenmai et al study the influence of the fertilization technology on the quality of radishes in the paper 'influence of cultivation density and fertilization treatment on main agronomic traits and yield of fruit radishes'.
In the research field of reducing cadmium content of vegetable crops, exogenous hormone is applied as one of main means, but the application of exogenous hormone is easy to cause soil deterioration, and hormone remained on the surface of crops can cause health safety problems.
Therefore, how to develop a radish cultivation technique which is easy to apply, environmentally friendly and safe, can effectively improve the core quality index of radish and can reduce the content of common heavy metal cadmium is urgently needed in the field.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a radish cultivation technology which is easy to apply, environment-friendly and safe, can effectively improve the core quality index of radishes and can reduce the content of common heavy metal cadmium.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a cultivation method for improving the quality of radishes comprises the following steps:
when two true leaves of radish seedlings are unfolded, applying shepherd's purse leavening to roots; the shepherd's purse leavening is obtained by fermenting shepherd's purse whole plant serving as a fermentation substrate and aspergillus niger A46 CGMCC No.3084 serving as zymophyte.
In the invention, the Aspergillus niger A46 CGMCC No.3084 is obtained from the market, and the invention does not relate to the screening or discovery of new strains.
As an embodiment of the invention, during fermentation of the shepherd's purse leavening, during the initial fermentation, a fermentation substrate is smashed and added with water to be stirred until the solid content is 60-70% (w/w).
As an embodiment of the invention, during fermentation of the shepherd's purse leavening, the inoculation amount of Aspergillus niger A46 CGMCC No.3084 is 3% (w/w), and the bacterial liquid used during inoculation is the bacterial liquid growing to the plateau stage.
In one embodiment of the invention, the water content of the shepherd's purse leavening is 20-30% (w/w).
In one embodiment of the invention, when the shepherdspurse herb leavening is applied to roots, the fertilizing amount is 80-100 kg/hm 2。
As an embodiment of the invention, before the Capsella bursa-pastoris fermentation product is applied to roots and at the beginning of radish cultivation, a compound fertilizer with N, P, K content of 15% is applied to soil as a base fertilizer, and the application amount of the compound fertilizer is 150-200 kg/hm2。
As an embodiment of the present invention, the radish is a garden wildness green radish.
The invention has the beneficial effects that:
the invention can well improve the biomass and the root weight of the radish; the crude fiber content can be obviously reduced, the protein content can be obviously improved, the malonaldehyde content can be obviously reduced, and the vitamin C content can be improved; the invention can also obviously reduce the accumulation of cadmium in the radish.
Detailed Description
The present invention is described in detail below by way of examples, and it should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.
Example 1
1. Raw materials:
radish: weixian county green radish
Soil: from Sichuan agricultural university experiment base (without heavy metal)
Compound fertilizer: on the basis of the decomposed cow dung, the content of N, P, K is adjusted to be 15 percent;
Fermentation strain: aspergillus niger A46 CGMCC No.3084, commercially available
Capsella bursa-pastoris: obtained on the market
2. Experimental method
(1) Smashing Capsella bursa-pastoris, and adding water until the solid content is 70% (w/w) to serve as a fermentation substrate; carrying out liquid culture on Aspergillus niger A46 CGMCC No.3084, taking the bacterial liquid growing to a platform stage as an inoculated bacterial liquid, and adding the inoculated bacterial liquid into a fermentation substrate, wherein the inoculation amount is 3% (w/w); spreading with film, and fermenting for 3 days; adjusting the water content to 27% (w/w) after the fermentation is finished, and taking the obtained product as the shepherd's purse fermentation product for later use.
(2) Filling soil to perform pot experiment on the radishes, and uniformly mixing a compound fertilizer into the soil after filling the soil, wherein the using amount of the compound fertilizer is 150kg/hm2。
(3) After planting radish seedlings, when the radish seedlings expand two true leaves, applying shepherd's purse leavening to the roots, wherein the application amount is 80kg/hm2。
3. Results of the experiment
After the radish is mature, the root weight, the total biomass, the crude fiber content, the protein content, the malondialdehyde content and the vitamin C content are detected. Taking the scheme in the experimental method as an experimental group, and taking the shepherd's purse leavening without application as a control group. When relevant experimental data are taken, three times of repetition are carried out, and an average value is taken. The results of the experiments are shown in the following table.
TABLE 1
Example 2
Based on example 1, CdCl2 & 2.5H is added into tested soil2O is analyzed to be pure, so that the concentration of cadmium in the soil is 5mg kg-1. On the basis of the above, the experiment of example 1 was repeated to examine the cadmium content in radish, and when the experimental data were related, three times of repetition were performed and the average value was obtained, and the results are shown in table 2.
TABLE 2
Claims (7)
1. A cultivation method for improving the quality of radishes is characterized by comprising the following steps:
when two true leaves of the radish seedling are unfolded, applying the shepherd's purse fermentation product to roots; the shepherd's purse leavening is obtained by fermenting the whole plant of shepherd's purse as a fermentation substrate and Aspergillus niger A46 CGMCC No.3084 as zymocyte.
2. The cultivation method as claimed in claim 1, wherein during fermentation of the shepherd's purse fermentation product, during initial fermentation, a fermentation substrate is smashed, and water is added to stir until the solid content is 60-70% (w/w).
3. The cultivation method as claimed in claim 1, wherein during fermentation of the shepherd's purse fermentation product, the inoculation amount of Aspergillus niger A46 CGMCC No.3084 is 3% (w/w), and the bacterial liquid used during inoculation is the bacterial liquid growing to the plateau stage.
4. The cultivation method according to claim 1, wherein the water content of the shepherd's purse fermentation product is 20-30% (w/w).
5. The cultivation method according to claim 1, wherein the amount of fertilizer applied when the Capsella bursa-pastoris fermentation product is applied at root is 80 to 100kg/hm2。
6. The cultivation method according to claim 1, wherein a compound fertilizer containing N, P, K% 15% is applied to soil as a base fertilizer before the Capsella bursa-pastoris fermentation product is applied and at the beginning of radish cultivation, and the application amount of the compound fertilizer is 150-200 kg/hm2。
7. The cultivation method according to claim 1, wherein the radish is an Weixian green radish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210474471.0A CN114747444A (en) | 2022-04-29 | 2022-04-29 | Cultivation method for improving radish quality |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210474471.0A CN114747444A (en) | 2022-04-29 | 2022-04-29 | Cultivation method for improving radish quality |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114747444A true CN114747444A (en) | 2022-07-15 |
Family
ID=82332507
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210474471.0A Pending CN114747444A (en) | 2022-04-29 | 2022-04-29 | Cultivation method for improving radish quality |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114747444A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101044837A (en) * | 2007-04-30 | 2007-10-03 | 中国农业科学院油料作物研究所 | Method for breeding hybrid of rape or cruciferae vegetables by using wild leaf mustard sterile cytoplasm |
| WO2013015666A1 (en) * | 2011-07-25 | 2013-01-31 | Jaime Lopez Cervantes | Method of extracting sulforaphane by means of a lactic fermentation treatment in cruciferous plants |
| CN103342597A (en) * | 2013-06-27 | 2013-10-09 | 重庆拓阳科技有限公司 | Preparation method of special compound microbial fertilizer for wheat for improving soil and product thereof |
| CN105481604A (en) * | 2015-10-16 | 2016-04-13 | 青岛海能海洋生物技术有限公司 | Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof |
| CN106077054A (en) * | 2016-06-12 | 2016-11-09 | 陆晓辉 | A kind of formula reducing Radix Raphani absorption heavy metal cadmium |
| CN107787754A (en) * | 2017-11-10 | 2018-03-13 | 四川农业大学 | A kind of method for promoting tree tomato to grow and reduce its aerial part cadmium content |
| CN107827654A (en) * | 2017-11-28 | 2018-03-23 | 芜湖四高农业科技有限公司 | A kind of radish composite fertilizer for improving soil texture |
| CN108307964A (en) * | 2018-03-05 | 2018-07-24 | 中国中医科学院中药研究所 | A kind of fine cultivating soil modification method in ginseng farmland |
| CN109848205A (en) * | 2019-01-23 | 2019-06-07 | 四川农业大学 | A method of application S. photeinocarpum stalk reduces romaine lettuce Cd accumulation |
-
2022
- 2022-04-29 CN CN202210474471.0A patent/CN114747444A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101044837A (en) * | 2007-04-30 | 2007-10-03 | 中国农业科学院油料作物研究所 | Method for breeding hybrid of rape or cruciferae vegetables by using wild leaf mustard sterile cytoplasm |
| WO2013015666A1 (en) * | 2011-07-25 | 2013-01-31 | Jaime Lopez Cervantes | Method of extracting sulforaphane by means of a lactic fermentation treatment in cruciferous plants |
| CN103342597A (en) * | 2013-06-27 | 2013-10-09 | 重庆拓阳科技有限公司 | Preparation method of special compound microbial fertilizer for wheat for improving soil and product thereof |
| CN105481604A (en) * | 2015-10-16 | 2016-04-13 | 青岛海能海洋生物技术有限公司 | Compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer and preparation method and application thereof |
| CN106077054A (en) * | 2016-06-12 | 2016-11-09 | 陆晓辉 | A kind of formula reducing Radix Raphani absorption heavy metal cadmium |
| CN107787754A (en) * | 2017-11-10 | 2018-03-13 | 四川农业大学 | A kind of method for promoting tree tomato to grow and reduce its aerial part cadmium content |
| CN107827654A (en) * | 2017-11-28 | 2018-03-23 | 芜湖四高农业科技有限公司 | A kind of radish composite fertilizer for improving soil texture |
| CN108307964A (en) * | 2018-03-05 | 2018-07-24 | 中国中医科学院中药研究所 | A kind of fine cultivating soil modification method in ginseng farmland |
| CN109848205A (en) * | 2019-01-23 | 2019-06-07 | 四川农业大学 | A method of application S. photeinocarpum stalk reduces romaine lettuce Cd accumulation |
Non-Patent Citations (12)
| Title |
|---|
| 宋以玲等: "生物菌水溶肥对土壤微生物、土壤酶活性及萝卜产量和品质的影响", 《山东农业科学》 * |
| 巨鹏等: "沼气发酵残留物对萝卜品质的影响", 《安徽农业科学》 * |
| 张美霞等: "越冬栽培与冬贮‘潍县青’萝卜的品质比较", 《北方园艺》 * |
| 朱红梅等: "稻草型生物有机肥对萝卜的作用效果", 《生态环境学报》 * |
| 李银平等: "浒苔生物有机肥的制备及其对樱桃萝卜品质影响的研究", 《食品工业科技》 * |
| 杨卓等: "微生物对植物修复重金属污染土壤的促进效果", 《应用生态学报》 * |
| 桑姝丽: "潍县萝卜配套栽培技术研究概述", 《潍坊高等职业教育》 * |
| 汪李平等: "有机蔬菜——根菜类生产技术规程", 《长江蔬菜》 * |
| 王朋成等: "生物菌剂Bio-Green对蔬菜作物的促生长作用研究", 《安徽农学通报》 * |
| 胡丽萍等: "组配改良剂对铅胁迫萝卜营养品质及铅积累的影响", 《浙江农业学报》 * |
| 董越勇等: "有机肥料对兰溪小萝卜生长及根际土壤肥力的影响", 《浙江农业学报》 * |
| 赵文彬等: "瑞拉菌素对10种作物幼苗生长的影响", 《西北农业学报》 * |
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