CN107963921A - A kind of Pleurotus eryngii fluid nutrient medium and cultural method for adding tea tree branch extracting solution - Google Patents
A kind of Pleurotus eryngii fluid nutrient medium and cultural method for adding tea tree branch extracting solution Download PDFInfo
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- CN107963921A CN107963921A CN201710894603.4A CN201710894603A CN107963921A CN 107963921 A CN107963921 A CN 107963921A CN 201710894603 A CN201710894603 A CN 201710894603A CN 107963921 A CN107963921 A CN 107963921A
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- tea tree
- tree branch
- extracting solution
- nutrient medium
- fluid nutrient
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of Pleurotus eryngii fluid nutrient medium and cultural method for adding tea tree branch extracting solution, using tea tree branch extracting solution as primary raw material, compound biogas slurry, agar, white sugar etc., which are mixed with, to be formed, compared to the prior art, the present invention prepares tea tree branch extracting solution using microwave extraction technology, takes short, short preparation period, the fluid nutrient medium production cycle is shortened, improves production efficiency；Containing itrogenous organic substances such as alkaloids in tea tree branch extracting solution, by preferred suitable additive amount, the carbon-nitrogen ratio of culture medium is moderate, and then promotes mycelial growth, then compatibility biogas slurry uses, and improves mycelia vigor, anti-miscellaneous bacteria disease ability enhancing；It is few that tea tree branch resource is used for component needed for Pleurotus eryngii production; it is equipped with simply, with short production cycle, the speed of bacterium germination is fast; cell age is consistent; fruiting is neat, and pollution rate is low, is conducive to scale and factorial praluction; for improving Pleurotus eryngii quality; increase tea grower's income, improve tea place added value, it may have very big realistic meaning.
Description
Technical field
The present invention relates to Pleurotus eryngii culture medium and technical field of cultivation, and in particular to a kind of apricot for adding tea tree branch extracting solution
Abalone mushroom fluid nutrient medium and cultural method.
Background technology
Pleurotus eryngii is a kind of precious all suitable macro fungi of food medicine, has aid digestion, reducing blood lipid, diuresis, improves human body
The functions such as immunity.More patterns using solid spawn culture at present, but the cycle length of this pattern production of hybrid seeds, cell age is inconsistent,
Fruiting is also irregular, is unfavorable for large-scale production, and in recent years, influence of the solid spawn cultivation lumber consumption to local ecology
Gradually highlight, gradual transition and upgrade finds new compost substitute for some areas；The cycle of the liquid spawn production of hybrid seeds is short,
The speed of bacterium germination is fast, and cell age is consistent, and fruiting is neat, and pollution rate is also low, is conducive to scale and factorial praluction, therefore use liquid
Body culture medium cultivation Pleurotus eryngii has important practical significance.
Contain suitable polysaccharide, itrogenous organic substance in tea tree branch（Amino acid, caffeine, catechin）With mineral matter etc. 600
Plurality of active ingredients, its chemical composition are similar to tea leaf.Tea tree branch is processed into the processing method of fluid nutrient medium more
It is rare, Liu Wen《The influence that various concentrations tea tree branch extracting solution grows agrocybe, dictyophora phalloidea, oyster mushroom, mushroom mycelium》It is middle by tea
Tree mushroom, dictyophora phalloidea, oyster mushroom, mushroom these four common edible mushroom parent species are inoculated into containing training made of various concentrations tea tree branch extracting solution
Support on base, by observing the growing state of hypha of edible fungus on culture medium, research tea tree branch extracting solution grows hypha of edible fungus
Influence.The result shows that tea tree branch extract concentration and growth potential, the speed of growth of mycelia have certain correlation；It is different dense
The tea tree branch extracting solution of degree plays the role of agrocybe to promote growth, has inhibitory action to mushroom；The branch extraction of high concentration tea tree
Liquid suppresses dictyophora phalloidea growth, plays the role of reducing the speed of growth to oyster mushroom but can promote growing way, and common tea tree branch extracting solution uses
Time-consuming for hot-water soak method, production cycle length.And also Pleurotus eryngii liquid is prepared without using tea tree branch extracting solution in the prior art
The research of culture medium.
The content of the invention
It is an object of the invention to provide it is a kind of add tea tree branch extracting solution Pleurotus eryngii fluid nutrient medium and cultural method,
With overcome the deficiencies in the prior art.
The object of the present invention is achieved like this：
A kind of Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution, it is characterised in that the fluid nutrient medium is to use tea tree
Branch extracting solution, biogas slurry, agar, white sugar, water are mixed with and form.
The fluid nutrient medium includes the material of following parts by weight：Tea tree branch extracting solution 90-100, biogas slurry 10-20,
Agar 8-10, white sugar 9-11, water 360-380.
The biogas slurry is to be derived from the anaerobic fermentation residues of normal operation methane-generating pit, filtered and after removing solid impurity
Liquid.
The tea tree branch extracting solution is made by following methods：
（1）Tea tree branch is crushed, is 1 according to solid-liquid ratio:15 addition extracting solutions stir and evenly mix first soak 16-18 it is small when, Ran Houjia
Heat boils 20-30min, is cooled to 75-80 DEG C, Microwave Extraction is carried out in Microwave Extraction instrument to seething with excitement, and obtains tea tree branch and slightly carries
Liquid, wherein the extraction conditions of the Microwave Extraction instrument are：Microwave frequency 2400MHZ, microwave power 500-600W, extraction time
8-10min；The extracting solution is a kind of, the volume fraction of ethanol wherein in ethanol water in water or ethanol water or ethanol
For 75-85%.
（2）Above-mentioned tea tree branch crude extract is first used into 4 layers of filtered through gauze, then is filtered, finally carries out gained filtrate very
Empty rotary evaporation is concentrated into the 40-60% of original volume, obtains tea tree branch extracting solution.
Utilize the method for the fluid nutrient medium cultivation Pleurotus eryngii, it is characterised in that comprise the following steps：
（1）Weigh tea tree branch extracting solution 90-100, biogas slurry 10-20, agar 8-10, white sugar 9-11, the water 360- of following parts by weight
380 are uniformly mixed configuration fluid nutrient medium；
（2）The good fluid nutrient medium of above-mentioned configuration is loaded into cultivating container；
（3）By step（2）In equipped with fluid nutrient medium cultivating container in 100-105 DEG C sterilize 20-30min, naturally cool to
Room temperature；
（4）First strain is inoculated into the culture dish equipped with PDA culture medium, 25 DEG C of progress rejuvenation cultures of constant temperature, then uses card punch
The 5mm inoculation blocks of identical cell age are punched from culture dish, are inoculated into step（3）Cultivating container in, be placed in it is nontoxic, pollution-free,
Cultivated in the cultivating chamber of no other strains.
The present invention has following beneficial effect：The present invention is compound biogas slurry, agar, white using tea tree branch extracting solution as primary raw material
Sugar etc., which is mixed with, to be formed, and compared to the prior art, the present invention prepares tea tree branch extracting solution using microwave extraction technology, takes
Short, short preparation period, shortens the fluid nutrient medium production cycle, improves production efficiency；The present invention utilizes tea tree branch extracting solution
For primary raw material, due to containing the itrogenous organic substances such as alkaloid in tea tree branch extracting solution, pass through preferred suitable additive amount, culture medium
Carbon-nitrogen ratio it is moderate, and then promote mycelial growth, then compatibility biogas slurry uses, and improves mycelia vigor, anti-miscellaneous bacteria disease ability increases
By force；The present invention is few for component needed for Pleurotus eryngii production by tea tree branch resource, is equipped with simple, with short production cycle, the speed of bacterium germination
It hurry up, cell age is consistent, and fruiting is neat, and pollution rate is also low, is conducive to scale and factorial praluction, for improving Pleurotus eryngii quality,
Increase tea grower's income, improve tea place added value, it may have very big realistic meaning.
Embodiment
A kind of Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution, it is characterised in that the fluid nutrient medium is to use
Tea tree branch extracting solution, biogas slurry, agar, white sugar, water are mixed with and form.
The fluid nutrient medium includes the material of following parts by weight：Tea tree branch extracting solution 90-100, biogas slurry 10-20,
Agar 8-10, white sugar 9-11, water 360-380.
The biogas slurry is to be derived from the anaerobic fermentation residues of normal operation methane-generating pit, filtered and after removing solid impurity
Liquid.
The tea tree branch extracting solution is made by following methods：
（1）Tea tree branch is crushed, is 1 according to solid-liquid ratio:15 addition extracting solutions stir and evenly mix first soak 16-18 it is small when, Ran Houjia
Heat boils 20-30min, is cooled to 75-80 DEG C, Microwave Extraction is carried out in Microwave Extraction instrument to seething with excitement, and obtains tea tree branch and slightly carries
Liquid, wherein the extraction conditions of the Microwave Extraction instrument are：Microwave frequency 2400MHZ, microwave power 500-600W, extraction time
8-10min；
（2）Above-mentioned tea tree branch crude extract is first used into 4 layers of filtered through gauze, then is filtered, finally by the progress vacuum rotation of gained filtrate
Turn to be concentrated by evaporation to the 40-60% of original volume, obtain tea tree branch extracting solution.
The extracting solution is a kind of, the volume fraction of ethanol wherein in ethanol water in water or ethanol water or ethanol
For 75-85%.
Utilize the method for the fluid nutrient medium cultivation Pleurotus eryngii, it is characterised in that comprise the following steps：
（1）Weigh tea tree branch extracting solution 90-100, biogas slurry 10-20, agar 8-10, white sugar 9-11, the water 360- of following parts by weight
380 are uniformly mixed configuration fluid nutrient medium；
（2）The good fluid nutrient medium of above-mentioned configuration is loaded into cultivating container；
（3）By step（2）In equipped with fluid nutrient medium cultivating container in 100-105 DEG C sterilize 20-30min, naturally cool to
Room temperature；
（4）First strain is inoculated into the culture dish equipped with PDA culture medium, 25 DEG C of progress rejuvenation cultures of constant temperature, then uses card punch
The 5mm inoculation blocks of identical cell age are punched from culture dish, are inoculated into step（3）Cultivating container in, be placed in it is nontoxic, pollution-free,
Cultivated in the cultivating chamber of no other strains.
Claims (6)
1. a kind of Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution, it is characterised in that the fluid nutrient medium is to use tea
Branch extracting solution, biogas slurry, agar, white sugar, water, which are mixed with, to be formed.
A kind of 2. Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution according to claim 1, it is characterised in that institute
The fluid nutrient medium stated includes the material of following parts by weight：It is tea tree branch extracting solution 90-100, biogas slurry 10-20, agar 8-10, white
Sugared 9-11, water 360-380.
3. a kind of Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution according to claim 1 or 2, its feature exist
In the biogas slurry is to be derived from the anaerobic fermentation residues of normal operation methane-generating pit, filtered and remove the liquid after solid impurity
Body.
4. a kind of Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution according to claim 1 or 2, its feature exist
In tea tree branch extracting solution is made by following methods：
（1）Tea tree branch is crushed, is 1 according to solid-liquid ratio:15 addition extracting solutions stir and evenly mix first soak 16-18 it is small when, Ran Houjia
Heat boils 20-30min, is cooled to 75-80 DEG C, Microwave Extraction is carried out in Microwave Extraction instrument to seething with excitement, and obtains tea tree branch and slightly carries
Liquid, wherein the extraction conditions of the Microwave Extraction instrument are：Microwave frequency 2400MHZ, microwave power 500-600W, extraction time
8-10min；
（2）Above-mentioned tea tree branch crude extract is first used into 4 layers of filtered through gauze, then is filtered, finally by the progress vacuum rotation of gained filtrate
Turn to be concentrated by evaporation to the 40-60% of original volume, obtain tea tree branch extracting solution.
A kind of 5. Pleurotus eryngii fluid nutrient medium for adding tea tree branch extracting solution according to claim 4, it is characterised in that institute
Extracting solution is stated to be a kind of in water or ethanol water or ethanol, the volume fraction of ethanol is 75-85% wherein in ethanol water.
6. utilize the method for the fluid nutrient medium cultivation Pleurotus eryngii described in claim 1-5 any one, it is characterised in that including
Following steps：
（1）Weigh tea tree branch extracting solution 90-100, biogas slurry 10-20, agar 8-10, white sugar 9-11, the water 360- of following parts by weight
380 are uniformly mixed configuration fluid nutrient medium；
（2）The good fluid nutrient medium of above-mentioned configuration is loaded into cultivating container；
（3）By step（2）In equipped with fluid nutrient medium cultivating container in 100-105 DEG C sterilize 20-30min, naturally cool to
Room temperature；
（4）First strain is inoculated into the culture dish equipped with PDA culture medium, 25 DEG C of progress rejuvenation cultures of constant temperature, then uses card punch
The 5mm inoculation blocks of identical cell age are punched from culture dish, are inoculated into step（3）Cultivating container in, be placed in it is nontoxic, pollution-free,
Cultivated in the cultivating chamber of no other strains.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710894603.4A CN107963921A (en) | 2017-09-28 | 2017-09-28 | A kind of Pleurotus eryngii fluid nutrient medium and cultural method for adding tea tree branch extracting solution |
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| CN201710894603.4A CN107963921A (en) | 2017-09-28 | 2017-09-28 | A kind of Pleurotus eryngii fluid nutrient medium and cultural method for adding tea tree branch extracting solution |
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| CN107963921A true CN107963921A (en) | 2018-04-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113039987A (en) * | 2021-03-25 | 2021-06-29 | 南阳琴溪湖农业开发有限公司 | Method for promoting rooting and survival of tea seedlings transplanted in dry alkaline land |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528117A (en) * | 2003-10-21 | 2004-09-15 | 中国科学院植物研究所 | Method for producing hedgehog fungus and special culture medium thereof |
| CN101265132A (en) * | 2008-04-25 | 2008-09-17 | 昆明理工大学 | Edible mushroom mother seed marsh liquid culture medium and mother seed manufacturing method |
| CN101317526A (en) * | 2007-06-06 | 2008-12-10 | 福建省农业科学院农业生态研究所 | Production and cultivation of glossy ganoderma with tea branch, tea, stem material |
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2017
- 2017-09-28 CN CN201710894603.4A patent/CN107963921A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528117A (en) * | 2003-10-21 | 2004-09-15 | 中国科学院植物研究所 | Method for producing hedgehog fungus and special culture medium thereof |
| CN101317526A (en) * | 2007-06-06 | 2008-12-10 | 福建省农业科学院农业生态研究所 | Production and cultivation of glossy ganoderma with tea branch, tea, stem material |
| CN101265132A (en) * | 2008-04-25 | 2008-09-17 | 昆明理工大学 | Edible mushroom mother seed marsh liquid culture medium and mother seed manufacturing method |
Non-Patent Citations (2)
| Title |
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| 刘文等: "不同浓度茶树枝提取液对茶树菇、竹荪、平菇、香菇菌丝生长的影响", 《世界科技研究与发展》 * |
| 郑生宏等: "茶树修剪作用与修剪枝的再利用", 《中国农业信息》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113039987A (en) * | 2021-03-25 | 2021-06-29 | 南阳琴溪湖农业开发有限公司 | Method for promoting rooting and survival of tea seedlings transplanted in dry alkaline land |
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Application publication date: 20180427 |
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