CN105475189B - Individual marking method in echinoderm BLUP method breeding process - Google Patents
Individual marking method in echinoderm BLUP method breeding process Download PDFInfo
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- CN105475189B CN105475189B CN201510874116.2A CN201510874116A CN105475189B CN 105475189 B CN105475189 B CN 105475189B CN 201510874116 A CN201510874116 A CN 201510874116A CN 105475189 B CN105475189 B CN 105475189B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
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Abstract
Individual marking method of the present invention, solve the problem of mark is difficult to echinoderm on traditional breeding method, individual mark easily can be made to echinoderms such as sea cucumber, sea urchins, in addition to can be with the growth traits of monitoring individual, simultaneously can also the feed coefficient etc. of recording individual the character that can not be recorded is marked using conventional fluorescent, and this mode, compared to traditional fluorescence labeling, its cost is also more cheap, it is also easier to distinguish chosen individual in seed selection.The appearance of this method, allowing echinoderm to carry out breeding using BLUP methods becomes possibility.It can be said that it possesses a variety of advantages, it is particularly suitable for popularization and application in the art, its market prospects is very wide.
Description
Technical field
The present invention relates to a kind of labeling method, individual mark side in particularly a kind of echinoderm BLUP method breeding process
Method.
Background technology
BLUP is BLUP（Best Linear Unbiased Prediction）, it is animals and plants selection
The individual breeding value method of estimation being most widely used in breeding, it can significantly improve the degree of accuracy of individual breeding value estimation, from
And significantly improve genetic progress.Since the seventies in last century, BLUP methods start to be widely used in the selection and use of livestock and poultry animal,
Turn into the conventional method of livestock and poultry animal selection and use in recent years.Although the selection and use research development of aquatic livestock is later,
Since recent two decades, BLUP methods are in the aquatic livestock such as bony fish such as Atlantic salmon, Tilapia mossambica and Penaeus Vannmei, spot
Preferably applied in the shell-fish such as section prawn.But for the species such as sea cucumber, sea urchin, BLUP methods are dfficult to apply to
Breeding is actual, and reason is that the species such as sea cucumber, sea urchin can not realize readable as what is implemented on the species such as bony fish and shell-fish
Individual mark（Fluorescence labeling etc.）, this causes the breeding value estimated can not be as seed selection according to applied to seed selection operation.Cause
This is in the cultivation of the echinoderms such as sea cucumber, sea urchin, breeding field, and the highly desirable one kind that finds can carry out individual mark to it
Method.
The content of the invention
The present invention is with low cost, the appearance in order to solve the above-mentioned deficiency present in prior art, propose that one kind is simple to operate
Easily distinguish the individual individual marking method chosen.
The present invention technical solution be：Individual marking method in a kind of echinoderm BLUP method breeding process, its
It is characterised by：Described method is followed the steps below：
A. when in breeding population sea cucumber body weight reach that more than 1g, sea urchin body weight reach more than 0.1g, to the sea of each family
Ginseng or sea urchin carry out grab sample, obtain family sample,
B. in units of individual, by each family sample be placed in plastics cultivation mesh bag in, mesh bag specification be more than or equal to 10cm ×
15cm, more than mesh 1mm, and escape from cultivated animals,
C. it is placed with mesh bag in centrifuge tube, pipe and places individual in the record mesh bag that taken notes with a pencil on 1 template scraps of paper, the scraps of paper
Family numbering and individual numbering, mesh bag plug-in plastic nameplate records family individual in mesh bag with marking pen on label and numbers
And individual numbering,
D. the plastic frame that mesh bag can be supported is put into mesh bag, the diameter of each side section of plastic frame is less than
5mm, and mesh bag mouthful is sealed using plastic fastener,
E. multiple mesh bags are connected in plastic ties, mesh bag spacing is 1-2 times of mesh bag width, and every plastic ties are entered
Line flag, while order of the mesh bag in plastic ties is recorded,
F. on two relative side walls of culturing pool, setting is multipair to match and the hook below the water surface, and same
Hook on the wall of side is in array, and each pair hook all makes a mark, and the distance between adjacent two hook is more than on the wall of the same side
The width of mesh bag,
G. the two ends of plastic ties are suspended on a pair of hooks respectively, stretched plastic rope makes its holding level, plastic ties with
Each pair links up with random pair,
When h. needing bait throwing in or measurement, plastic ties are taken out from water, the plastic fastener at mesh bag mouthful is pulled open, bait is carried out
That expects is fed, or individual in mesh bag is taken out, and character measurement and note are carried out to individual after recording its family numbering and individual numbering
Record,
I. periodically plastic ties and hook again random pair are adjusted, and records adjustment result, adjustment time interval is less than
30 days,
J. cultivated using recirculated water,
When k. choosing seeds, by the individual breeding value of production performance test result calculations, find breeding value and reach breeding objective
The family number and individual of individual are numbered, according to the pair record and mesh bag of plastic ties and the last time of hook in plastic ties
Journal can find the individual from culturing pool, and carry out offspring and breed.
In described Step d, attachment base is put into mesh bag.
The present invention compared with the existing technology, has the following advantages that：
Individual marking method of the present invention, solves the problem of being difficult to mark to echinoderm on traditional breeding method, energy
It is enough that individual mark easily is made to echinoderms such as sea cucumber, sea urchins, in addition to can be with the growth traits of monitoring individual, simultaneously
Can also the feed coefficient etc. of recording individual mark the character that can not be recorded using conventional fluorescent, and this mode compared to
Traditional fluorescence labeling, its cost is also more cheap, is also easier to distinguish chosen individual in seed selection.This method goes out
Existing, allowing echinoderm to carry out breeding using BLUP methods becomes possibility.It can be said that it possesses a variety of advantages, especially fit
Together in popularization and application in the art, its market prospects is very wide.
Brief description of the drawings
Fig. 1 is the view of the culturing pool used in the embodiment of the present invention.
Embodiment
Below in conjunction with the embodiment of the brief description of the drawings present invention.As shown in Figure 1：
Individual marking method in a kind of echinoderm BLUP method breeding process, is followed the steps below：
When sea urchin body weight reaches more than 0.1g in breeding population, grab sample is carried out to the sea urchin of 50 familys, respectively taken
Sample number is 50.
In units of individual, each family sea urchin is placed in plastics cultivation mesh bag, mesh bag specification is 15cm × 20cm, mesh
For 1mm.
Place to place in the centrifuge tube that specification is 1.5ml, pipe in mesh bag and taken notes with a pencil on 1 template scraps of paper, the scraps of paper
Record on family numbering and individual numbering individual in mesh bag, the plug-in plastic nameplate of mesh bag, label with marking pen record mesh bag
The family numbering and individual numbering of body.
Plastic frame is put into mesh bag, is supported, to obtain the requisite space of sea urchin activity, this frame of plastic
The circular cross-section on each side of frame, its a diameter of 4mm, and sealed mesh bag mouthful using plastic fastener.
It is random 50 mesh bags are selected from all 2500 plastics mesh bags to be series at the 1st plastic ties, mesh bag spacing 15cm,
The plastic ties are labeled as with S1, and relative position of the mesh bag on rope is recorded according to mesh bag internal labeling（It is suitable i.e. in plastic ties
Sequence）.Altogether using 50 plastic ties, using the method series connection mesh bag of the 1st plastic ties, second rope is labeled as S2, other ropes
Mark the like.
20 tons of breeding pools are chosen, part is respectively uniformly distributed 64 hooks, shape below its two relative side wall water surface
It is distributed into 64 hooks on 64 pairs of hooks, and each side wall in array, on the wall of the same side between adjacent two hook
Distance is 20cm, and each pair hook does same mark（It is designated as G1-G64）.
All plastic ties two ends for connecting mesh bag are hung in breeding pool on a pair of hooks, stretching makes it keep water
It is flat, plastic ties and the 50 hook random pairs selected at random.
During bait throwing in, plastic ties are taken out from water, mesh bag slide fastener is pulled open, sea-tangle is fed.
Individual in mesh bag was taken out one by one every 30 days, after record family numbering and individual numbering, character is carried out to individual
Measurement and record.After measurement, to plastic ties and hook random pair adjustment again.
Cultivated using recirculated water, make other physical and chemical indexs of breeding water in addition to hydraulic pressure, illumination completely the same.
Production performance test result is analyzed using computer, individual breeding value is calculated, to all living individuals
Breeding value carry out ranking, record preceding 100 individual family numbers and individual numbered, it is demonstrated experimentally that according to plastic ties with linking up with
The journal in plastic ties of pair record and mesh bag of last time find the individual extremely convenient from breeding pool, should
100 individuals are used as the parent of family of future generation.
Claims (2)
1. individual marking method in a kind of echinoderm BLUP method breeding process, it is characterised in that：Described method according to
Lower step is carried out：
A. when in breeding population sea cucumber body weight reach that more than 1g, sea urchin body weight reach more than 0.1g, sea cucumber to each family or
Sea urchin carries out grab sample, obtains family sample,
B. in units of individual, each family sample is placed in plastics cultivation mesh bag, mesh bag specification is more than or equal to 10cm × 15cm,
More than mesh 1mm, and escape from cultivated animals,
C. it is placed with mesh bag and family individual in the record mesh bag that taken notes with a pencil on 1 template scraps of paper, the scraps of paper is placed in centrifuge tube, pipe
System's numbering and individual numbering, mesh bag plug-in plastic nameplate record family numbering individual in mesh bag and individual on label with marking pen
Body is numbered,
D. the plastic frame that mesh bag can be supported is put into mesh bag, the diameter of each side section of plastic frame is less than 5mm,
And mesh bag mouthful is sealed using plastic fastener,
E. multiple mesh bags are connected in plastic ties, mesh bag spacing is 1-2 times of mesh bag width, and enters rower to every plastic ties
Note, while order of the mesh bag in plastic ties is recorded,
F. on two relative side walls of culturing pool, setting is multipair to match and the hook below the water surface, and the same side wall
On hook be in array, each pair hook all make a mark, and on the wall of the same side it is adjacent two hook the distance between be more than mesh bag
Width,
G. the two ends of plastic ties are suspended on a pair of hooks respectively, stretched plastic rope makes its holding level, plastic ties and each pair
Link up with random pair,
When h. needing bait throwing in or measurement, plastic ties are taken out from water, the plastic fastener at mesh bag mouthful is pulled open, bait is carried out
Feed, or individual in mesh bag taken out, character measurement and record are carried out to individual after recording its family numbering and individual numbering,
I. periodically plastic ties and hook again random pair are adjusted, and record adjustment result, adjustment time interval is less than 30 days,
J. cultivated using recirculated water,
When k. choosing seeds, by the individual breeding value of production performance test result calculations, the individual that breeding value reaches breeding objective is found
Family number and individual number, according to the order in plastic ties of pair record and mesh bag of plastic ties and the last time of hook
Record can find the individual from culturing pool, and carry out offspring and breed.
2. individual marking method in echinoderm BLUP method breeding process according to claim 1, it is characterised in that：
In described Step d, attachment base is put into mesh bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510874116.2A CN105475189B (en) | 2015-12-03 | 2015-12-03 | Individual marking method in echinoderm BLUP method breeding process |
Applications Claiming Priority (1)
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| CN201510874116.2A CN105475189B (en) | 2015-12-03 | 2015-12-03 | Individual marking method in echinoderm BLUP method breeding process |
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| Publication Number | Publication Date |
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| CN105475189A CN105475189A (en) | 2016-04-13 |
| CN105475189B true CN105475189B (en) | 2017-11-07 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642060B (en) * | 2018-05-30 | 2019-10-11 | 中国海洋大学 | Application of the LAS1 gene in saponin(e high yield type sea cucumber breeding |
| CN110278899B (en) | 2019-07-17 | 2022-02-25 | 漳澳国际有限公司 | Single shellfish multi-bag floating-sinking type aquaculture frame |
| CN113287553A (en) * | 2021-06-29 | 2021-08-24 | 广西海洋研究所有限责任公司 | Marettes margaritifera marking and measuring breeding cage |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4133294A (en) * | 1977-04-27 | 1979-01-09 | University Of Delaware | Process for marking molluscs |
| CN1449656A (en) * | 2002-04-08 | 2003-10-22 | 李思发 | Breeding technology for Megalobrama amblycephala pujiang No.1 model |
| CN101091458A (en) * | 2007-07-08 | 2007-12-26 | 李成林 | Method for breeding fast growing bechedemer |
| CN201557455U (en) * | 2009-11-23 | 2010-08-25 | 大连水产学院 | Sea urchin individual marking device |
| CN101731167A (en) * | 2009-12-28 | 2010-06-16 | 中国水产科学研究院黄海水产研究所 | Marking method of selective breeding individual of turbot |
| CN102106294A (en) * | 2011-02-12 | 2011-06-29 | 大连圣贤水产养殖有限公司 | Deep water well greenhouse type sea cucumber breeding method |
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