CN1449656A - Breeding technology for Megalobrama amblycephala pujiang No.1 model - Google Patents
Breeding technology for Megalobrama amblycephala pujiang No.1 model Download PDFInfo
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Abstract
The present invention provides a method for breeding improved varielies of fishes, and said method includes the following steps: a). cultivating the fishes to be bred and selected; b). in the period of fish growth selecting fishes with large body and good ratio of body length and body weight from fish group, in which its selection rate is less than 5/10000; c). cultivating selected fishes to make them reproduce next generation of fishes; and d). repeating step b and c for 3-10 times. Said invented method can effectively breed various fishes. The Megalobrana amblycephala pujiang No.1 model bred by said invented method not only is quick in growth, good in body form, high in purity, but also can meet the requirements for green food.
Description
Technical field
The present invention relates to the breeding technique of fish breeding, relate to No. 1 breeding technique of megalobrama amblycephala Pujiang particularly.
Background technology
Over more than 20 year, China's fish breeding kind research has obtained than good result, shows following several respects:
1. crossbreeding
By crossbreeding technology, from more than 100 group hybrid combinations, successively filtered out the tangible hybrid combination of following hybrid vigour: as rich carp (the red carp ♀ * scattered mirror carp of making the country prosperous
), carp (the red carp ♀ of pocket * Yuanjiang River carp of lotus unit
), triple-crossing carp (the carp ♀ of lotus unit * mirror carp
), high mountain carp (lotus look red carp ♀ * Xiangjiang wild carps
), the lotus carp (scattered mirror carp ♀ * red carp of making the country prosperous
), the good fortune longevity fish (Mozambique Tilapia mossambica ♀ * bolti
), Buddhist nun fish (bolti ♀ * Oreochromis aureus difficult to understand
) etc.
2. colony's seed selection
Colony's seed selection also claims to mix seed selection, is a kind of traditional selection.Generally need through the above preferred ability success of continuous six generations.China successively breeds the red carp of pocket, make the country prosperous red carp, Pengzhe fish etc.The seed selection of these fish lasts and all is no more than 10 years, and technology is single, only limits to phenotype.
3. chromosome set engineering breeding
China has obtained all female carps, hybridized prussian carp, Hunan cloud carp with chromosome set engineering breeding technique, Hunan cloud crucian carp etc., and wherein all female carps is that the gynogenesis technology combines with property commentaries on classics technology and gets; Hunan cloud carp and Hunan cloud crucian carp then are to utilize the distant hybridization of carp crucian carp to obtain allotetraploid, form by triploid carp or crucian carp between tetraploid and diplontic carp or crucian mating acquisition doubly again.
4. comprehensive breeding
The comprehensive breeding technology is meant carries out selection cross earlier, again in conjunction with technology such as artificial induction's gynogenesiss, makes merit in stable.China cultivates jian carp, clever carp, loose Pu pond crucian carp, high and cold carp etc.
5. genetic engineering breeding
China Aquatic Science Research Institute's Heilungkiang aquatic products research changes the chum salmon growth hormone gene over to carp.Inst. of Hydrobiology, Chinese Academy of Sciences changes the growth hormone gene of grass carp and carp over to carp, crucian.Now all in the safety pilot scale stage.
In the fish species of China's seed selection, examine (recognizing) fixed pass through also few through the former breeding of national aquatic products validation board so far, only have: the red carp of pocket, the red carp of making the country prosperous, jian carp, loose Pu carp, German mirror carp breeding line, the cold-resistant strain of the red carp of pocket, Pengzhe fish, loose Pu pond crucian carp, and No. 1, megalobrama amblycephala Pujiang.These kinds relate generally to carp and crucian carp.The kind from relating to no matter, still kind of a number is seen, all can not meet the needs of far away.Breeding remains the maximum weak link of China's culture fishery.
To what fish had been bred so far more than 20 kind arranged in the world.The Dao Naxun rainbow trout that the U.S. is arranged of having adopted colony's breeding technique to select, the Atlantic salmon of Norway etc.Adopted what the selection cross technology selected GIFT Tilapia mossambica etc. arranged.Yet, because the fish sexual maturity generally takes 3～4 years, add seed selection 4 generations needs 12～16 years, needed for 6～7 generations as reaching inheritance stability, need 18～28 years, so the seed selection cycle is long.
Megalobrama amblycephala originates in some large and medium-sized lakes in middle reaches, the Changjiang river, and nineteen fifty-five is found, and becomes China gradually and mainly culture one of object.Megalobrama amblycephala is the fresh-water fishes that quite are subjected to various places people favor, and is especially well-known at home and abroad because of the poem of Mao Zedong " just drink Yangtze River Water, eat blunt snout bream again ".In the freshwater aquiculture output in area, Shanghai, megalobrama amblycephala accounts for 15%.And in the whole nation, its output reached 510,000 tons in 2000, occupied the 6th of cultured freshwater fish.Since this fish is raised and train, comparatively serious degradation phenomena has appearred behind the megalobrama amblycephala cultured population of various places, and show as growth rate and slow down, sexual maturity is done sth. in advance, the elongated attenuation of the bodily form etc.
Therefore, breeding production presses for breeding.And the seed selection of breeding presses for the technology that the exploitation seed selection is effective and stable, the seed selection cycle is short; Tracking and monitoring is bred the technology of breeding genetic variation, sets up the technology of molecular genetic sign.
Summary of the invention
The purpose of this invention is to provide a kind of new fish breeding method, it is the integrated technology of colony's seed selection and biotechnology.
In a first aspect of the present invention, a kind of seed selection fish breeding method is provided, it is characterized in that it comprises step:
(a) culture the fish for the treatment of seed selection;
(b) between sashimi (raw fish) is long-term, from the shoal of fish, choose individual big, body weight gain than good fish, wherein selection rate is less than 5/10000ths;
(c) fish of selecting in the step (b) is cultured, made its breeding produce fish of future generation;
(d) repeating step (b) and (c) 3-10 time.
In a preference, selection rate is 0.00001-0.0005 in step (b).
In another preference, selection rate is for equaling 0.0004 in step (b).
In another preference, in step (c), choose the fish of fast growth more than 10%.
In another preference, in step (d) afterwards, also comprise step:
(e) make the stable and/or purifying of the piscinity shape of selecting by the gynogenesis method.
In another preference, less than 7.5%, average heterozygosity is less than 0.05 in polymorphic seat ratio for the population genetic degree of variation of the fish of seed selection; Average genetic resemblance degree is greater than 90% between the individuality of the fish of seed selection in colony.
In another preference, described fish is selected from down group: megalobrama amblycephala, bolti, Chinese soft-shelled turtle.
In another preference, also be included in step (c) afterwards, with the molecular genetic sign fish of selecting is identified.
In another preference, also be included in step (e) afterwards, with the molecular genetic sign fish of stable and/or purifying is identified.
In another preference, repeating step (b) and (c) 4-6 time in step (d).In another preference, also be included in reinforced cultivating in the step (d), shorten the seed selection period technique.
Description of drawings
Fig. 1 is the photo of a kind of megalobrama amblycephala of institute's seed selection in a kind of preference of the present invention.
Fig. 2 is the megalobrama amblycephala gynogenesis prime fish photo of institute's seed selection in the another kind of preference of the present invention.
Fig. 3-5 has shown that the fish to seed selection carries out the photo that the molecular genetic sign is identified.Wherein Fig. 3 has shown the molecular genetic marker of megalobrama amblycephala Pujiang No. 1 (1-8) and mud lake original seed (9-15) thereof; Fig. 4 has shown the molecular genetic marker of megalobrama amblycephala Pujiang No. 1 (9-16) and gynogenesis fishes (1-8) thereof; Fig. 5 has shown the molecular genetic marker of megalobrama amblycephala Pujiang No. 1 (9-16) and gynogenesis fishes (1-8) thereof.
Embodiment
Technology path of the present invention is that colony's seed selection and biotechnology height are integrated.Key has three: one, at the lower characteristics of megalobrama amblycephala genetic diversity, selection by superhigh intensity, fish is carried out continual seed selection, it is fast to select growth by generation, individual big and and the comparatively desirable fish of body length/weight ratio, to the 6th generation, obtain growth rate than the obvious raising of original seed (30%), the good breeding of build.The 2nd, with cytogenetics and molecular genetic techniques, the genetic variation degree of quantitative analysis breeding variety makes it inheritance stability and purifying by gynogenesis, sets up the distinctive molecular genetic marker of breeding variety.The 3rd, by reinforced cultivating, shorten 3 years fish breeding cycles.
The method of seed selection fish breeding of the present invention comprises: high strength colony continues breeding technique; Genetic variation monitoring and molecular genetic sign biotechnology; Hot housing is educated; Shorten aspects such as seed selection period technique:
(a) select to lay a foundation colony.From natural population, select the lay a foundation colony of a kind of colony preferably (for example growth is very fast, and build is better) as seed selection; Set up breeding line and control series;
(b) colony's seed selection and biotechnology height are integrated.At first, breeding line is operated according to the program of strictness according to the Quantitative Genetics principle; To the 6th generation (F
6), promptly growth rate is significantly improved, and build is more satisfactory, and genetic character is used molecular genetic techniques to set up its genetic marker, and by the gynogenesis technology it stablized and purifying when stablize.
(c) colony's breeding technique.Set up 2 parallel breeding lines.It breeds effectively big or small each about 100 groups of colonies.Between from the fry to the sexual maturing period, at 4～5cm summer flower (20～30 age in days), 12～15cm fingerling (120 age in days), 500g adult fish (2 age) and 750～1000g parent (3 age) stage, respectively by 5%, 10%, 12% and 50% the rate of selecting and remain, choose fish individual big, build good (body length/height ratio is 2.0～2.2) from the shoal of fish, always selection rate is 3/10000ths.Carry out repeatedly by above-mentioned steps.By for seed selection and use the randomised block design method that choosing system and control series are done tracking and testing and assessment.Comparatively good until the breeding line growth rate than be significantly improved (30%), build of control series, and during inheritance stability.So continue the seed selection of 6 generations.
(d) biotechnology.Use cytogenetics and molecular genetic techniques to measure the genetic variation of breeding variety.Set up the molecular genetic marker of breeding.Obtain the breeding of purifying by the gynogenesis technology.
(e) reinforced cultivating shortens the seed selection period technique.The fish sexual maturity generally needs 3～4 years.Use 6 generations of traditional selection seed selection will 18～24 years.The seed selection excessive cycle is the major reason that influences the fish breeding progress.The inventor finds in research process, by reinforced cultivating, megalobrama amblycephala 2 years also can be ripe and artificial induced spawning breed, thereby can shorten the seed selection cycle.Megalobrama amblycephala 6 generation seed selection 18 years in the original plan now shortens to 15 years.
In an example of the present invention, a little less than, the hereditary variability narrow and small at megalobrama amblycephala natural distribution district, characteristics such as heritability is low, according to the quantitative inheritance theory, carry out 3/10000ths high strength selection, adhered to long-term breeding 15 years, obtained growth rate and improved 30%, the effect of average annual seed selection effect 5.9% than original seed.On this basis, further use the chromosome operating technology, obtained the gynogenesis fishes of No. 1, Pujiang, make genetic character further stable, be able to purifying.
In an object lesson of the present invention, whole seed selection process has 2 Main Stage:
Colony's seed selection stage: be to select, obtain growth rate and obviously improve (30%), the good offspring of build by 15 years, the high-intensity colony in 6 generations, per generation 3/10000ths;
The biotechnology breeding stage: be from F
5Generation beginning is stablized and purifying by the gynogenesis means, and the purity (in the colony between individuality genetic resemblance degree) of the shoal of fish is further brought up to more than 94%.
In addition, the inventor also uses randomly amplified polymorphic DNA (randomly amplified polymorphicDNA abbreviates RAPD as) scientific discovery and has set up the distinctive molecular genetic marker of breeding, has set up discrimination method fast and accurately on this basis.
The fishing gear that selects with the inventive method has that growth is fast, build is good, purity is high and meet pollution-free food requires characteristics such as (based on the fresh forage of throwing something and feeding, manufactured feed are auxilliary, and disease is few, and medication is few).For example, in the seed selection of megalobrama amblycephala being carried out with the inventive method, a kind of breeding of acquisition, promptly the megalobrama amblycephala Pujiang has the following advantages for No. 1:
(1) growth is fast
No. 1, megalobrama amblycephala Pujiang is under general aqiuculrue condition, and then the stage, average daily gain 0.5g raised through 100 days (7～October), can reach 50～60g.Two age grade sections, average daily gain 3.0g raised through 200 days (5～October), can reach 500～600g.
(2) purity height
No. 1 population genetic degree of variation of megalobrama amblycephala Pujiang is: polymorphic seat ratio 5.88%, average heterozygosity 0.0258 (isodynamic enzyme measurement result); Average genetic resemblance degree 93.5% (DNA RAPD measurement result) between individuality in the colony.
Genetic distance is again than its parent (F in the colony of No. 1 gynogenesis fishes of Pujiang
6) colony in genetic distance reduce 46%, promptly be further purified.
(3) conform to the pollution-free food requirement
The megalobrama amblycephala Pujiang is a staple food with the grass No. 1, and disease is few, and medication is few.Conform to safety, hygienic conditions, pollution-free green food requirement.
Innovative point of the present invention is:
(1) it is integrated not see colony's seed selection and biotechnology height both at home and abroad as yet, promptly obtains obviously in seed selection
On the basis of effect, further by modern biotechnology stablized, the similar techniques of purifying.(1) (2) are guidance with the Quantitative Genetics theory, and 3/10000ths high strength is chosen in both at home and abroad
Still beyond example.(3) application cell heredity and molecular genetic techniques, the genetic variation degree of quantitative analysis breeding variety,
Set up the distinctive molecular genetic marker of breeding variety, make it further by the gynogenesis technology
Stablize and purifying, this integrated technology does not still have report in the fish breeding at home and abroad.
(4) by reinforced cultivating, the fish breeding cycle can be shortened effectively.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1
The lay a foundation acquisition of colony of fish to be selected
1985 and 1986, introduce megalobrama amblycephala original seed parent population from mud lake, Hubei Province (30,000 mu) for continuous two batches.The 1985 years end were introduced raun 15 tails, and milter 15 tails (surviving number) will be introduced raun 46 tails the end of the year 1986 again, milter 112 tails (surviving number).Half that raises up seed with this two batches of fish next years is breeding line.Adopt traditional colony's selection, set up two breeding lines respectively.Second half is mixed into control series.Introduced fingerling 2000 tails then in 1992 again from this lake, add control series, in case its gene pool is lost in the possibility of propagating artificially in the environment.
Embodiment 2
The seed selection of megalobrama amblycephala
In the breeding line that in embodiment 1, obtains, by generation good big individual of stalwartness and build of selecting and remain, per generation the rate of selecting and remain always from the fry to the parent population be about 3/10000ths.Obtained the 6th generation (F in 1998 and 1999
6).Pujiang is for No. 1 that growth vigor is obvious, the 6th generation (F of the seed selection of stabilization characteristics of genetics
6).
Test is mainly carried out in Shanghai Aquatic Products Univ. 9CN) Nanhui fish germplasm experiment station.Specialize in 5 in the fishpond that the megalobrama amblycephala seed selection uses, totally 16 mu, 24 (every 28m of concrete floor
2).In seed selection, carry out the comparative trial of growth performance by generation.Test method is to adopt the completely random block design, carries out the same pool (pond) of breeding line and control series and compare in number pond (pool).Cut the fin mark.Calculate the rate of body weight gain and the survival rate of an age, two ages and three age grade sections.Each comparative test repeated once in 1 year.Comparative trial continued to calendar year 2001 from 1986.
Embodiment 3
Evaluation to the breeding line fish
(1) growth rate
In Jiangsu, Zhejiang one band aqiuculrue environment (megalobrama amblycephala, silver carp, flathead, crucian carp etc. are raised together with, and per mu yield 500～750kg uses cobalt blue pigment and particulate material), No. 1 megalobrama amblycephala of Pujiang is the stage then, average daily gain 0.5g raised through 100 days (7～October), and body weight can reach 50～60g.Two age grade sections, average daily gain 3.0g raised through 200 days (5～October), and body weight can reach 500～600g.Three age grade sections, average daily gain 2.7g raised through 200 days (5～October), and body weight reaches more than the 1000g.
In the lake net culture in enclosure fish environment of separating (monoculture megalobrama amblycephala), throw in the 50g fingerling end of the year-beginning of the year, just reached more than the 500g before National Day, have the advantage (other megalobrama amblycephalas take 1～1.5 month evening and just reach same specification) in pollution-free food and season.
(2) body length/height ratio remains on 2.1～2.2, meat thick (Fig. 1).Body surface pearl color and luster is beautiful.
(3) hereditary capacity
A. karyotype formulas:
2N＝48，26m+18sm+4st，NF＝92。With the original seed unanimity.
B. population genetic degree of variation:
According to the isodynamic enzyme measurement result, calculating polymorphic seat ratio is 5.88%, and average heterozygosity is 0.0258.The result that measures of nineteen ninety is that the polymorphic seat of the megalobrama amblycephala ratio in Liangzi Lake, mud lake and South Lake (Wuhan) is 20%, and average heterozygosity is 0.0808～0.0851; And the megalobrama amblycephala of Hanjiang should be 13.3%, 0.0549 mutually.Show No. 1 existing big purifying of Pujiang.
C. average genetic resemblance degree between individuality in the colony: according to the RAPD measurement result is 93.5%.Wild population does not still have comparable data.
Embodiment 4
Pilot experiment
Be to detect the seed selection effect, the lake megalobrama amblycephala seed multiplication farm of separating of the national Jiangsu Province Changzhou in the Shanghai City Jiading District is hoped new aquatic products seed multiplication farm and established carries out pilot experiment.
(1) the Shanghai City Jiading District is hoped new aquatic products seed multiplication farm:
From 1994, the annual introduction about breeding variety parent 500 tails of the present invention, by 1998 update all the megalobrama amblycephala parent population of this field.Spawning rate that No. 1, Pujiang and emergence rate have improved more than 20% than the spawning rate and the emergence rate of original megalobrama amblycephala that uses.
(2) Jiangsu Province's Changzhou lake megalobrama amblycephala seed multiplication farm of separating
1998, this was listed in national aquatic products seed multiplication farm construction project.This selects the govern-house-variety of No. 1 megalobrama amblycephala of Pujiang for this for use.Began in 1998 to introduce.Played a large amount of breeding of beginning seed in 1999, the needs of supply lake, neighbouring area culture in enclosure and aqiuculrue.Because this breeding improves more than 20% than original used megalobrama amblycephala growth rate, to go on the market one month earlier, price is higher more than 20% than the joint back.
Embodiment 5
The foundation of pure lines
Chromosome set method of operating is routinely set up pure lines with the megalobrama amblycephala that obtains among the embodiment 2.1999 and 2000 are respectively from megalobrama amblycephala F
5And F
6Gynogenesis fishes 150 tails and 1500 tails (Fig. 2) have been produced.Calendar year 2001 has obtained son's generation of gynogenesis fishes again, and its genetic character is further stable.
Embodiment 6
The molecular genetic marker of No. 1 megalobrama amblycephala of Pujiang
Use the RAPD technology, synthesized many different primers, carry out DNA cloning.Find that primer S43 (GTCGCCGTGA), S8 (GTCCACACCGG) and S18 (CCACAGCAGT) are especially suitable for use as the primer of amplification No. 1 megalobrama amblycephala of Pujiang and gynogenesis fishes thereof.
Wherein, use primer S43 that the amplification of megalobrama amblycephala Pujiang No. 1 (1-8) and mud lake original seed (9-15) thereof is seen Fig. 3, the molecular genetic marker band is positioned at 1140bp, 990bp and 850bp place.
Use primer S8 that the amplification of megalobrama amblycephala Pujiang No. 1 (9-16) and gynogenesis fishes (1-8) thereof is seen Fig. 4, the molecular genetic marker band is positioned at the 1100bp place.
Use primer S18 that the amplification of megalobrama amblycephala Pujiang No. 1 (9-16) and gynogenesis fishes (1-8) thereof is seen Fig. 4, the molecular genetic marker band is positioned at the 860bp place.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after having read above-mentioned instruction content of the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. the method for a seed selection fish breeding is characterized in that it comprises step:
(a) culture the fish for the treatment of seed selection;
(b) between sashimi (raw fish) is long-term, from the shoal of fish, choose individual big, body weight gain than good fish, wherein selection rate is less than 5/10000ths;
(c) fish of selecting in the step (b) is cultured, made its breeding produce fish of future generation;
(d) repeating step (b) and (c) 3-10 time.
2. the method for claim 1 is characterized in that, selection rate is 0.00001-0.0005 in step (b).
3. method as claimed in claim 2 is characterized in that, selection rate is for equaling 0.0004 in step (b).
4. the method for claim 1 is characterized in that, chooses the fish of fast growth more than 10% in step (c).
5. the method for claim 1 is characterized in that, in step (d) afterwards, also comprises step:
(e) make the stable and/or purifying of the piscinity shape of selecting by the gynogenesis method.
6. as claim 1 or 5 described methods, it is characterized in that less than 7.5%, average heterozygosity is less than 0.05 in polymorphic seat ratio for the population genetic degree of variation of the fish of seed selection; Average genetic resemblance degree is greater than 90% between the individuality of the fish of seed selection in colony.
7. the method for claim 1 is characterized in that, described fish is selected from down group: megalobrama amblycephala, bolti, Chinese soft-shelled turtle.
8. the method for claim 1 is characterized in that, also is included in step (c) afterwards, with the molecular genetic sign fish of selecting is identified.
9. method as claimed in claim 5 is characterized in that, also is included in step (e) afterwards, with the molecular genetic sign fish of stable and/or purifying is identified.
10. the method for claim 1 is characterized in that, repeating step (b) and (c) 4-6 time in step (d).
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889561A (en) * | 2010-07-23 | 2010-11-24 | 广西壮族自治区水产研究所 | Selection method of new cold-resistant species of tilapia |
| CN102349462A (en) * | 2011-08-30 | 2012-02-15 | 吴江市水产养殖有限公司 | Breeding method for improved variety of fishes |
| CN102599079A (en) * | 2011-01-24 | 2012-07-25 | 华中农业大学 | Rapid high-efficiency method for constructing half-sib family of Megalobrama Terminalis |
| CN105145434A (en) * | 2015-09-12 | 2015-12-16 | 上海海洋大学 | Screening and combining method for megalobrama amblycephala advantage combining |
| CN105165679A (en) * | 2015-09-12 | 2015-12-23 | 上海海洋大学 | Establishing and breeding method for three matched breeding lines of megalobrama amblycephala |
| CN105475189A (en) * | 2015-12-03 | 2016-04-13 | 大连海洋大学 | Individual marking method applied in echinoderm breeding process achieved through BLUP method |
| CN110150185A (en) * | 2019-05-28 | 2019-08-23 | 中国三峡建设管理有限公司 | A kind of thickness jaw triangular bream seed method for massively culturing |
| CN111328740A (en) * | 2020-03-17 | 2020-06-26 | 中国水产科学研究院长江水产研究所 | Breeding method for rapidly growing silver carps and application thereof |
| CN111657186A (en) * | 2020-05-28 | 2020-09-15 | 湖南师范大学 | Method for cultivating natural gynogenesis megalobrama amblycephala |
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2002
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889561A (en) * | 2010-07-23 | 2010-11-24 | 广西壮族自治区水产研究所 | Selection method of new cold-resistant species of tilapia |
| CN102599079A (en) * | 2011-01-24 | 2012-07-25 | 华中农业大学 | Rapid high-efficiency method for constructing half-sib family of Megalobrama Terminalis |
| CN102599079B (en) * | 2011-01-24 | 2013-11-27 | 华中农业大学 | Method for constructing half-sib family of Megalobrama Terminalis |
| CN102349462A (en) * | 2011-08-30 | 2012-02-15 | 吴江市水产养殖有限公司 | Breeding method for improved variety of fishes |
| CN105145434A (en) * | 2015-09-12 | 2015-12-16 | 上海海洋大学 | Screening and combining method for megalobrama amblycephala advantage combining |
| CN105165679A (en) * | 2015-09-12 | 2015-12-23 | 上海海洋大学 | Establishing and breeding method for three matched breeding lines of megalobrama amblycephala |
| CN105475189A (en) * | 2015-12-03 | 2016-04-13 | 大连海洋大学 | Individual marking method applied in echinoderm breeding process achieved through BLUP method |
| CN110150185A (en) * | 2019-05-28 | 2019-08-23 | 中国三峡建设管理有限公司 | A kind of thickness jaw triangular bream seed method for massively culturing |
| CN110150185B (en) * | 2019-05-28 | 2021-08-10 | 中国三峡建设管理有限公司 | Large-scale cultivation method for megalobrama amblycephala fries |
| CN111328740A (en) * | 2020-03-17 | 2020-06-26 | 中国水产科学研究院长江水产研究所 | Breeding method for rapidly growing silver carps and application thereof |
| CN111657186A (en) * | 2020-05-28 | 2020-09-15 | 湖南师范大学 | Method for cultivating natural gynogenesis megalobrama amblycephala |
| CN111657186B (en) * | 2020-05-28 | 2022-02-18 | 湖南师范大学 | Method for cultivating natural gynogenesis megalobrama amblycephala |
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