CN113661956B - Rapid evaluation method for high-throughput abalone high-temperature resistance - Google Patents
Rapid evaluation method for high-throughput abalone high-temperature resistance Download PDFInfo
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- 238000011156 evaluation Methods 0.000 title claims abstract description 44
- 241000237891 Haliotidae Species 0.000 claims abstract description 53
- 239000013535 sea water Substances 0.000 claims abstract description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000000034 method Methods 0.000 claims abstract description 20
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 16
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K61/00—Culture of aquatic animals
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- A01K61/51—Culture of aquatic animals of shellfish of gastropods, e.g. abalones or turban snails
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
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- A—HUMAN NECESSITIES
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Abstract
A rapid evaluation method for high-throughput abalone high-temperature resistance relates to the technical field of aquatic organisms. The device for evaluating the high-temperature resistance of the abalones comprises a culture box, a seawater temperature regulator, an oxygen generator, a protein separator, a temperature monitor, a water pump and an attachment plate. The physical characteristics that the abalones have wide and developed abdomens, the abdomens have strong suction force and can be firmly adsorbed on the smooth surface are utilized, the time that the foot muscles gradually lose adsorption capacity under continuous high-temperature stress of the abalones is taken as an indication, and the tolerance capacity of different abalones to high water temperature is evaluated. The method is simple and convenient to operate, large in number of single test samples, short in evaluation period, and free from damage to tested abalones due to evaluation work, and the abalone samples subjected to evaluation can be used for carrying out subsequent breeding work, so that the method is beneficial to improving the breeding efficiency of high-temperature-resistant varieties of abalones. The influence of the environment on the tested abalone sample can be avoided, the test result precision is high, and the evaluation work of the high-temperature resistance of the abalone can be carried out in a large quantity.
Description
Technical Field
The invention relates to an evaluation technology for high-temperature resistance of abalones in the technical field of aquatic organisms, in particular to a high-throughput quick evaluation method for high-temperature resistance of abalones, which distinguishes different varieties or different culture groups of abalones.
Background
China is the first major country of abalone culture in the world, the culture yield accounts for more than 85% of the global yield, in 2019, the abalone yield in China exceeds 18 million tons, wherein Fujian is taken as the main culture area of abalones in China, the annual yield of the abalones in Fujian province accounts for 82.7% of the national yield, the annual yield value is about 200 million yuan, the abalone culture has been developed into the first mariculture variety of the output value in the Fujian province, and the main culture variety is the haliotis discus hannai originally produced from Liaoning and Shandong coasts. The Haliotis discus hannai is originally a temperate species in a Huang Bohai sea area of a temperate zone, the high temperature resistance of the Haliotis discus hannai is improved after genetic improvement, fujian is moved to the south to develop cultivation, the Fujian abalone industry is rapidly developed, but limited by the original ecological habit of the Haliotis discus hannai and global warming and the like, sudden death of the cultured Haliotis discus hannai due to high temperature intolerance occurs in part of the cultured sea areas in summer every year, and the method becomes one of the main bottlenecks which disturb the healthy development of the Haliotis discus Haliotis culturing industry at present. Therefore, the cultivation of new species (line) of high temperature resistant abalone is very important, and how to quickly and accurately evaluate the high temperature resistance of the cultivated line in the cultivation process of the high temperature resistant variety is very important.
The traditional evaluation method for the high-temperature resistance of the abalones is mostly based on summer culture data or a laboratory evaluation method represented by methods such as semi-lethal temperature and the like, the summer culture data in the natural sea area is used as an index, the evaluation period is long, the hydrological conditions in the natural sea area are complex, the evaluation result is easily influenced by other factors, accurate data cannot be provided, and the reliability is low; the laboratory evaluation method represented by methods such as a semi-lethal temperature and the like generally needs to change the water temperature in a short time and finish the evaluation work by taking the survival rate of the abalones as an index, but the method has great damage to the abalones, individuals with strong tolerance often cannot survive after the evaluation is finished, so that the experimental abalones die, and although relevant evaluation data is obtained, breeding individuals cannot be obtained for carrying out subsequent breeding work.
Chinese patent CN 107372273A discloses a method for determining the temperature resistance of a quickly distinguished abalone, which comprises temporarily culturing a sample of an abalone to be evaluated under a suitable water temperature condition, fixing an infrared sensor on a shell of the abalone corresponding to the accurate position of the heart of the abalone by using a non-invasive method, determining the heart rate of the abalone measured in a non-invasive manner, recording Bao Xinlv under different temperature conditions in real time, obtaining an Arrhenius curve, and calculating the ABT value of each sample, thereby determining the difference of the heat tolerance of each sample. In addition, when the operation steps are carried out, the tested abalone sample needs to be separated from the originally adapted culture seawater environment, when the operation is improper, the sample is frightened by operations such as prying and the like, or the activity of the tested abalone is influenced due to exposure, and if the subsequent clean seawater recovery process is not proper, the measured result is very easy to deviate. In addition, the method needs to use an infrared sensor to be adhered to the abalone shell, the number of samples which can be tested by the same operator each time is limited by the number of the sensors, the adhering proficiency, the time for putting back into clean seawater to recover and the like, only dozens of samples can be tested each time, and the evaluation work of one group needs about 10-20 days. The operation process is still more complicated, error evaluation results are easy to occur, and the large-scale breeding work of the high-temperature resistant abalone variety is not facilitated. Therefore, how to evaluate the high temperature resistance of the abalone rapidly, accurately and in high flux in a laboratory becomes one of the problems that abalone breeders need to solve urgently.
The abalone possesses wide and developed ventral foot, the foot muscle structure makes it capable of being adsorbed firmly to smooth rock surface in deep underwater place, and the adsorption force has high strength. Under the continuous high-temperature stress, the foot muscles gradually lose the adsorption capacity and are separated from attachments, so the attachment capacity of the abalones is taken as an important index for judging the health state of the abalones.
Disclosure of Invention
The invention aims to provide the evaluation device for developing the high-temperature resistance of the abalones, which has the advantages of simple and convenient operation, large number of single test samples, short evaluation period, no damage to tested abalones caused by evaluation work, capability of developing subsequent breeding work by using the evaluated abalones samples and the like.
The invention also aims to provide a rapid evaluation method for high-throughput abalone high-temperature resistance, which is beneficial to improving the breeding efficiency of high-temperature resistant varieties of abalones.
The device for evaluating the high-temperature resistance of the abalones comprises a culture box, a seawater temperature regulator, an oxygen generator, a protein separator, a temperature monitor, a water pump and an attachment plate; the culture box is connected with the protein separator, the water pump and the seawater temperature regulator through a PVC water pipe; an oxygen generator is arranged between the protein separator and the water pump and is used for filling sufficient oxygen into the culture seawater; the device comprises a culture box, a water pump, a protein separator, a temperature controller, a temperature detector, a temperature probe and a temperature sensor, wherein seawater is filled in the culture box and used for culturing an abalone to be tested, the water pump pushes the seawater to circulate, the effluent seawater is treated by the protein separator and is regulated by the seawater temperature controller to a temporary culture water temperature and then flows into the culture box again, the temperature detector is connected with one end of the temperature probe, and the other end of the temperature probe is arranged below the seawater liquid level and used for monitoring the temperature in the culture box. At least two lifting ropes for fixing the attachment plates are arranged at the top of the culture box and used for pulling the attachment plates to be vertical from the plane.
The aquaculture tank can adopt a conventional aquaculture aquarium, and different sizes can be selected according to the number of the abalone samples to be evaluated.
The attachment plate can be made of an acrylic plastic plate, and the surface of the plate is smooth; the tail part of the attachment plate is provided with a through hole for inserting the lifting rope so as to be beneficial to hanging the lifting rope.
A method for rapidly evaluating high-throughput abalone high-temperature resistance comprises the following steps:
1) Selecting abalones of different types or varieties, labeling the abalones to be evaluated, measuring Bao Yangpin growth parameters, temporarily culturing in a culture box, setting water temperature and salinity, feeding fresh gracilaria, asparagus or kelp, replacing one fourth of fresh seawater every day, timely cleaning residual baits and excrement, and feeding for 5-15 days to ensure that the abalones to be evaluated are in a normal state;
in the step 1), setting the water temperature and the salinity, controlling the water temperature to be 18-22 ℃, controlling the salinity to be 30-35, controlling the concentration of dissolved oxygen to be not less than 6mg/L and controlling the pH to be not less than 8.0; the water temperature is preferably 20 ℃.
2) When temporarily culturing to the 7 th day, putting the attachment plate for evaluation into the bottom of the culture box, gradually raising the temperature of the attachment plate for evaluation from 18 ℃ at a speed of 1 ℃/h, vertically suspending the attachment plate attached with the abalones in the culture seawater at 28 ℃, and then continuously raising the temperature of the culture seawater to a final stress temperature of 30-34 ℃;
in step 2), the final stress temperature is preferably 32 ℃.
3) Starting timing when the water temperature reaches the final stress temperature, and recording the time that each individual loses the adhesive capacity and falls off from the adhesive plate, namely the time for maintaining the adsorption capacity at high temperature; transferring the individuals falling off after losing the adhesive ability into fresh seawater for recovery, wherein the initial water temperature of the fresh seawater is 14-18 ℃, the recovery time is 3 days, the temperature is raised by 1 ℃ every day, and the death condition within 3 days is recorded; the whole evaluation process is carried out in a dark room, and when a light source is needed for observing and moving the abalone sample, the wavelength of the used light source is 550-600 nm; statistical analysis of the data was performed using span 22, plotted using GraphPad Prism 8 and R-package.
The invention discloses a high-flux and convenient-to-operate method for evaluating the high-temperature resistance of abalones based on the specific attachment capacity of the abalones different from other main-cultured shellfish, which utilizes the physical characteristics that the abalones have wide and developed ventral legs, have strong suction force of the ventral legs and can be firmly adsorbed on a smooth surface, and evaluates the high-temperature resistance of different abalones by taking the time that the foot muscles gradually lose the adsorption capacity and can be separated from attachments as an indication under the continuous high-temperature stress of the abalones. The method has the advantages of simple and convenient operation, large number of single test samples, short evaluation period, no damage to tested abalones due to evaluation work, capability of developing subsequent breeding work of the abalones samples after evaluation, contribution to improving the breeding efficiency of high-temperature resistant varieties of the abalones, and the like. The method can avoid the influence of the environment on the tested abalone sample, has high precision of the test result, and can carry out the evaluation work of the high temperature resistance of the abalone in a large quantity.
Drawings
FIG. 1 is a schematic structural view of a device for evaluating the resistance of a fire-resistant abalone.
FIG. 2 is a graph comparing the duration of heat stress attachment (HAD) of southern and northern Haliotis discus hannai ino.
FIG. 3 is a graph showing a comparison of heat stress attachment duration (HAD) of Haliotis discus hannai, and Haliotis discus hannai.
Detailed Description
The following examples will further illustrate and describe the technical solutions of the present invention with reference to the accompanying drawings. Commercially available products may be used unless otherwise specified.
As shown in figure 1, the evaluation device for carrying out the high temperature resistance of the abalone comprises a culture box (12), a seawater temperature regulator (1), an oxygen generator (2), a protein separator (3), a temperature monitor (5), a water pump (13) and an attachment plate (8); at least two lifting ropes (7) for fixing the attachment plates are arranged at the top of the culture box (12) and used for pulling the attachment plates to be vertical from the plane; the culture box (12) is connected with the protein separator (3), the water pump (13) and the seawater temperature regulator (1) through a PVC water pipe (11); an oxygen generator (2) is arranged between the protein separator (3) and the water pump (13) and is used for filling sufficient oxygen into the culture seawater; the aquaculture device is characterized in that seawater (10) is filled in the aquaculture box (12) and used for aquaculture of abalones to be tested and evaluated (9), a water pump (13) pushes the seawater to circulate, the flowing-out seawater (10) is firstly treated by a protein separator (3) and then regulated to a temporary aquaculture water temperature by a seawater temperature controller (1) and then flows into the aquaculture box (12) again, the temperature detector (5) is connected with one end of a temperature probe (4), and the other end of the temperature probe (4) is arranged below a seawater liquid level (6) and used for monitoring the temperature in the aquaculture box.
The breeding tank (12) can adopt a conventional breeding aquarium, and different sizes can be selected according to the number of samples of the abalones to be evaluated.
The attachment plate (8) can be made of an acrylic plastic plate, and the surface of the plate is smooth. The tail part of the attachment plate (8) is correspondingly provided with a through hole for inserting the lifting rope (7) so as to be beneficial to hanging.
The embodiment of the rapid evaluation method for the high-temperature resistance of the high-flux abalone comprises the following steps:
1) When carrying out the high temperature resistance assessment of the abalones, selecting different types or varieties of abalones, labeling samples of the abalones (9) to be assessed, measuring the growth parameters of Bao Yangpin, temporarily culturing the abalones in a culture tank, wherein the culture tank is a conventional culture aquarium and can select different sizes according to the number of the samples of the abalones to be assessed. Controlling the water temperature in the culture box at 20 ℃, controlling the salinity at 30-35, filling sufficient oxygen into culture seawater by an oxygen generator (2), ensuring that the concentration of dissolved oxygen is not lower than 6mg/L and the pH is not lower than 8.0, feeding fresh gracilaria, asparagus or kelp, replacing one fourth of fresh seawater every day, cleaning residual bait and excrement in time, feeding and feeding for 5-15 days to ensure that the tested abalone is in a normal state;
the method comprises the steps that an abalone to be tested is cultured in a culture box (12), seawater (10) is filled in the culture box, the seawater is pushed by a water pump to circulate, the effluent seawater (10) is treated by a protein separator (3) at first, is regulated to temporary culture water temperature by a seawater temperature controller (1) and then flows into the culture box (12) again, the temporary culture water temperature is controlled at 20 ℃, the salinity is 30-35, the concentration of dissolved oxygen is not lower than 6mg/L, the pH is not lower than 8.0, fresh gracilaria, asparagus or kelp are fed, one fourth of fresh seawater is replaced every day, residual bait and excrement are cleaned in time, and the abalone is cultured for 5-15 days to ensure that the tested abalone is in a normal state.
The protein separator can be selected from the manufacturers and models comprising: ansu ger environmental protection equipment ltd, model: GEER-DB5.
The oxygen generator can select manufacturers: shandong remote graphic Environment technology, inc., model No.: YT-9000-10P.
2) When temporarily culturing to the 7 th day, placing the attachment plate (8) for evaluation at the bottom of the culture box (12), gradually raising the temperature from 20 ℃ at a rate of 1 ℃/h after the tested abalones gradually crawl to the attachment plate for evaluation, vertically suspending the attachment plate with the abalones in the culture seawater at the temperature of 28 ℃, and then continuously raising the temperature of the culture seawater to the final stress temperature of 32 ℃.
3) Counting time from the water temperature reaching 32 ℃, and recording the time when each individual loses the adhesive capacity and falls off from the adhesive plate, namely the time for maintaining the adsorption capacity at high temperature, namely heat stress adhesion duration (HAD); the individuals lost the ability to adhere were transferred to fresh seawater at an initial water temperature of 16 ℃ for 3 days, allowed to rise 1 ℃ per day, and their death status was recorded for 3 days. The whole evaluation process is carried out in a dark room, and when a light source is needed for observing and moving the abalone sample, the wavelength of the used light source is 550-600nm. Statistical analysis of the data was performed using span 22, plotted using GraphPad Prism 8 and R-package.
Specific examples are given below.
Example 1:
and (3) evaluating the high temperature resistance of the southern population of the haliotis discus hannai (code number: YX) and the northern population of the haliotis discus hannai (code number: DL), and comparing the high temperature resistance of the two populations.
Taking 50 samples of the abalone in the south population of the haliotis discus hannai and 50 samples of the abalone in the north population of the haliotis discus hannai respectively, sticking a label on each abalone shell, temporarily culturing the samples in a high-temperature resistance capacity evaluation device of the abalones, controlling the average shell length of the southern population of the haliotis discus hannai to be 5.3cm, controlling the average shell length of the northern population of the haliotis discus hannai to be 5.5cm, controlling the temperature of seawater in the evaluation device to be 20 ℃, controlling the salinity of the seawater to be 33, controlling the dissolved oxygen concentration to be 6.2mg/L and controlling the pH to be 8.1, feeding fresh asparagus, replacing one fourth of the fresh seawater every day, timely cleaning residual baits and excrement, feeding for 10 days normally, and confirming that the tested abalones are in a normal state; and (3) temporarily culturing until the 7 th day, putting the attachment plate for evaluation into the bottom of the tank, gradually raising the temperature of the tested abalone from 20 ℃ at a speed of 1 ℃/h, vertically suspending the attachment plate attached with the abalone in the culture water body at 28 ℃, and then continuously raising the water temperature in the culture tank to 32 ℃.
The time from the time of reaching 32 ℃ was counted, and the time at which each individual lost the adhesive ability and dropped from the adhesive plate, that is, the time at which the adsorption ability was maintained at high temperature (HAD), was recorded. And (3) moving the falling individual losing the adhesive ability to fresh seawater at 16 ℃ in time for recovery, carrying out recovery observation on the individual in a period of 3 days, wherein the recovery time is 3 days, the temperature rises by 1 ℃ every day, and recording the death condition within 3 days. The whole evaluation is carried out in a dark room, and when a light source is needed for observing and moving the abalone sample, the wavelength of the used light source is 550-600nm. Statistical analysis of the data was performed using span 22, plotted using GraphPad Prism 8 and R-package.
The HAD measurements of the southern and northern haliotis discus hannai populations are shown in fig. 2. As can be seen from fig. 2, the south population is higher than the north population on HAD50 (YX: 4.96h dl 3.49h), also 5.49 ± 2.47h significantly higher than the north population 4.06 ± 2.49h on average of HAD (P =0.019 straw 0.05); the north-south population has larger difference in the coefficient of variation, and the numerical value is YX:47.85% less than DL:68.88%, which reflects that the southern population is more uniform and more concentrated in falling after long-time southern high-temperature domestication; although the initial dropping time is within 1h, the maximum attachment duration of the southern population (0.72-11.27 h) is obviously higher than that of the northern population (0.34-10.02), which indicates that the southern population has higher high-temperature resistance and has high-temperature breeding potential.
Example 2:
the high temperature resistance of Haliotis discus hannai (code number: DD), haliotis diversicolor (code number: FF) and Haliotis discus hannai (code number: FF) are evaluated and compared.
Taking 60 samples of Haliotis discus hannai, haliotis diversicolor and Haliotis diversicolor, sticking a label on each shell of the Haliotis diversicolor and temporarily culturing in a culture box of an evaluation device for high temperature resistance of the Haliotis diversicolor, wherein the average shell length of the Haliotis diversicolor is 7.8cm, the average shell length of the Haliotis diversicolor is 8.1cm, the average shell length of the Haliotis diversicolor is 7.4cm, the temperature of seawater in the evaluation device is 20 deg.C, the salinity of the seawater is 32, the dissolved oxygen concentration is 6.5mg/L, the pH is 8.1, feeding fresh thallus Gracilariae, replacing one fourth of fresh seawater every day, cleaning up residual bait and feces in time, normally feeding for 9 days, and confirming that the abalone to be tested is in a normal state; and (3) temporarily culturing until the 7 th day, putting the attachment plate for evaluation into the cylinder bottom, gradually raising the temperature at the speed of 1 ℃/h from 20 ℃ after the tested abalones gradually creep to the attachment plate for evaluation, vertically suspending the attachment plate attached with the abalones in the culture water body at the temperature of 28 ℃, and then continuously raising the water temperature in the culture box to 33 ℃.
The time from when the water temperature reached 33 ℃ was counted, and the time at which each individual lost the adhesive ability and dropped from the adhesive plate, that is, the time at which the adsorption ability was maintained at high temperature (HAD), was recorded. And (3) timely transferring the individuals lost the adhesive ability to fresh seawater at 17 ℃ for recovery, carrying out recovery observation on the individuals in a period of 3 days, wherein the recovery time is 3 days, the temperature rises by 1 ℃ every day, and recording the death condition within 3 days. The whole evaluation process is carried out in a dark room, and when a light source is needed for observing and moving the abalone sample, the wavelength of the used light source is 550-600nm. Statistical analysis of the data was performed using SPASS 22, plotted using GraphPad Prism 8 and packet R.
The HAD of Haliotis discus hannai (DD), haliotis Diversicolor (DF), and Haliotis diversicolor (FF) at 32 deg.C is shown in FIG. 3.DD the longest adsorption time (10.97 h) was at a minimum at the initial drop time (0.42 h), HAD50 (4.95 h), average drop time (5.11. + -. 2.40 h); DF is better than the other two Bao Da in initial falling time (0.79 h), HAD50 (7.47 h), average adsorption time (7.26 +/-3.53 h) and coefficient of variation (0.49); the HAD mean (6.89 ± 3.23 h) and median (6.89 h) values of FF were close, significantly higher than DD (P =0.015 straw 0.05).
As can be seen from fig. 3, haliotis discus hannai (DD) has the minimum of the initial falling time (0.42 h), HAD50 (4.95 h), average falling time (5.11 ± 2.40 h), and the longest adsorption time (10.97 h); the initial falling time (0.79 h) of the Haliotis Diversicolor (DF), the HAD50 (7.47 h), the average adsorption time (7.26 +/-3.53 h) and the coefficient of variation (0.49) are all higher than those of the other two types Bao Da; the HAD mean value (6.89 ± 3.23 h) and median number (6.89 h) of haliotis diversicolor (FF) were close and significantly higher than haliotis diversicolor (DD) (P =0.015 stra @ -0.05). The result shows that the haliotis discus hannai (DD) has the lowest high temperature resistance, the hybrid species haliotis Discus (DF) has higher high temperature resistance, and the heterosis is shown.
The invention is not limited to the examples described above, but rather, variations and equivalent modifications are possible within the true spirit and scope of the invention.
Claims (5)
1. A rapid evaluation method for high-throughput abalone high-temperature resistance is characterized by comprising the following steps:
1) Selecting abalones of different types or varieties, labeling the abalones to be evaluated, measuring Bao Yangpin growth parameters, temporarily culturing in a culture box, setting water temperature and salinity, feeding fresh gracilaria, asparagus or kelp, replacing one fourth of fresh seawater every day, timely cleaning residual baits and excrement, and feeding for 5-15 days to ensure that the abalones to be evaluated are in a normal state;
2) When temporarily culturing is carried out for 7 days, the attachment plate for evaluation is placed at the bottom of the culture box, after the tested abalones gradually crawl to the attachment plate for evaluation, the temperature is gradually increased from 18 ℃ at the speed of 1 ℃/h, the attachment plate attached with the abalones is vertically suspended in culture seawater at 28 ℃, and then the culture seawater is continuously increased to the final stress temperature of 30-34 ℃;
3) Starting timing when the water temperature reaches the final stress temperature, and recording the time that each individual loses the adhesive capacity and falls off from the adhesive plate, namely the time for maintaining the adsorption capacity at high temperature; transferring the individuals lost the adhesive ability into fresh seawater for recovery, wherein the initial water temperature of the fresh seawater is 14-18 ℃, the recovery time is 3 days, the temperature rises by 1 ℃ every day, and the death condition within 3 days is recorded; the whole evaluation process is carried out in a dark room, and when a light source is needed for observing and moving the abalone sample, the wavelength of the used light source is 550-600 nm; statistical analysis of the data was performed using span 22, plotted using GraphPad Prism 8 and R-package.
2. The method for rapidly evaluating the high-throughput abalone temperature resistance as claimed in claim 1, wherein in step 1), the water temperature and salinity are set, the water temperature is controlled at 20 ℃, the salinity is 30-35, the dissolved oxygen concentration is not less than 6mg/L, and the pH is not less than 8.0.
3. The method for rapidly evaluating the high-throughput abalone temperature resistance according to claim 1, wherein in step 1), the cultivation tank is a conventional cultivation aquarium sold in the market, and different sizes are selected according to the number of samples of abalones to be evaluated.
4. The method for rapidly evaluating the high-throughput abalone temperature resistance according to claim 1, wherein in step 2), the attachment plate is made of acrylic plastic plate, and the surface of the plate is smooth.
5. The method for rapidly evaluating the high temperature resistance of high-throughput abalone according to claim 1, wherein in step 2), the final stress temperature is 32 ℃.
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