CN107372273A - A kind of quick assay method for distinguishing Bao heat resistance - Google Patents
A kind of quick assay method for distinguishing Bao heat resistance Download PDFInfo
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- CN107372273A CN107372273A CN201710797269.0A CN201710797269A CN107372273A CN 107372273 A CN107372273 A CN 107372273A CN 201710797269 A CN201710797269 A CN 201710797269A CN 107372273 A CN107372273 A CN 107372273A
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- bao
- heart rate
- heat resistance
- distinguishing
- assay method
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
Abstract
A kind of quick assay method for distinguishing Bao heat resistance, is related to aquatic biological heat resistance measure.Abalone sample to be measured is temporarily supported in circulation, cleaning is fed and feeds；Shell attachment is removed, is dried；The accurate location of Bao heart is sought using infrared sensor, signal intensity is not less than 1V, sensor is fixed on Bao shell after determining position；Bao, which is put back in seawater, to recover, and using water-bath, heating treatment, until Bao heart rate declines, stops heating, Bao is transferred in normal seawater, record the change of Bao heart rate and corresponding temperature in real time；The heart rate of acquisition is handled with temperature data, it is then that the longitudinal axis draws Arrhenius curves using temperature as transverse axis, heart rate, linear regression is carried out to two sections of curves before and after knee of curve, obtains regression equation, simultaneous equations, the abscissa of intersection point is the heart rate ABT values of Bao；Randomly select healthy individuals to be used to determine, average for the heat tolerance for characterizing each colony.
Description
Technical field
The present invention relates to aquatic biological heat resistance measure, more particularly, to a kind of quick measure for distinguishing Bao heat resistance
Method.
Background technology
Since ancient times, Bao always is the rare food materials of China, occupies first of marine products eight delicacies.China is the eighties in last century
End has started the large-scale farming of Bao, and by the end of the year 2013, more than 100,000 tons, the direct economy output value has surpassed hundred for its annual production
Hundred million.Haliotis discus hannai Ino (Haliotis discus hannai) original is the main breed variety in northern China sea area, in recent years, with
The successful incubation of new varieties " Dalian No.1 " and the breakthrough renewal of cultural technique, it cultivates scope constantly south and expanded, present Fujian
The haliotis discus hannai Ino culture zone of largest domestic, its annual production up to national yield 80%.Although domestication is for many years in south,
The heat-resisting ability of haliotis discus hannai Ino is not still strong, and the event of summer mortality takes place frequently, and with global warming and cultivation ring
The continuous deterioration in border, this case will be more severe.Thus, the high temperature resistant new varieties (being) for cultivating Bao are particularly important, and
The accurate assessment method for wherein establishing the high temperature resistant character of Bao is the key of breed of variety.
The research of traditional Bao Naiwen characters be mostly cultivation data based on summer or using the methods of semilethal low temperature as
Evaluating method in the laboratory of representative, the former grows experimental period, and hydrology complicated condition in natural waterses, experiment easily by other because
Element influences, and can not provide accurate data, and, as assessment method in the laboratory of representative, generally to exist the methods of semilethal low temperature
Change water temperature in short time, complete assessment work using the survival rate of Bao as index, but damage of this method to Bao is very big, comments
After survey terminates, the strong individual of tolerance often can not also survive, and cause death of the experiment with Bao.Therefore, how in laboratory
Rapidly and accurately evaluate and test one of the heat resistance, the problem of turning into Bao breeder's urgent need to resolve of Bao.
1889, Svante Arrhenius proposed an Arrhenius equation, for illustrating that chemical reaction rate is normal
Relation between number and temperature change.In certain temperature range, it is proportionate between the metaboilic level and temperature of poikilotherm,
Thus Arrhenius equations are also applied for describing the relation between some physiological reactions and temperature.For example, Stillman etc.
(Stillman JH, 1996) calculates porcelain crabs using the relation between the heart rate of measure, oxygen demand and temperature
ABT (Arrhenius break temperatures)；Dong etc. (Dong and Williams, 2011) is by determining the heart
Relation between rate and temperature, the difference that two kinds of differences transfer ABT between female's relative is compared, and (Prusina et are quoted for Prusina
Al., 2014), as evaluation biology to one of standard of temperature tolerance.
Evaluating method based on heart rate has many advantages, such as, as experiment condition is controllable, the cycle is short, good economy performance, to individual nothing
Damage, thus, will be able to be the training of Bao Naiwen new varieties (being) if developing the assessment method of Bao heat resistance based on technique
Educate and huge facility is provided.
The content of the invention
It is an object of the invention to provide short, easy to operate, the good economy performance with the test and appraisal cycle, and abalone will not be used to experiment
Cause to damage, a kind of quick measure side for distinguishing Bao heat resistance for the advantages that corresponding assessment work can be completed in laboratory
Method.
The present invention comprises the following steps：
1) abalone sample to be measured is temporarily supported in circulation, feeds fresh fragrant plant mentioned in ancient texts or sea-tangle, in time clear up residual bait with
Excrement, feed；
2) by abalone sample to be measured, the attachment on its shell is removed, abalone shell is dried；
3) method that the heart rate measurement of Bao uses non-invasive, the accurate location of Bao heart is sought using infrared sensor,
Signal intensity is not less than 1V, infrared sensor is fixed on Bao shell using glue after determining position；
4) Bao for fixing infrared sensor is put back in clean seawater and recovered, for supplementing Bao in operation
The moisture of loss, then using water-bath, heating treatment is carried out with fixed rate, until Bao heart rate is remarkably decreased, stops heating, will
Bao is transferred in normal seawater, in continuous mode, records the change of Bao heart rate and corresponding temperature in real time；
5) heart rate of acquisition is handled with temperature data, is then that the longitudinal axis is drawn using temperature as transverse axis, heart rate
Arrhenius curves, linear regression is carried out to two sections of curves respectively before and after Arrhenius points of inflexion on a curve, obtained corresponding
Regression equation, two equations of simultaneous, the abscissa of intersection point is the heart rate ABT values of Bao；
6) in each tested colony, no less than 20 healthy individuals is randomly selected and are used to determine, are averaged for table
Levy the heat tolerance of each colony.
In step 1), the temperature in the circulation can be 16~25 DEG C, and salinity can be 30 ‰~35 ‰, concentration
Not less than 6mg/L；Nursing time can be 5~15 days, to ensure that abalone sample to be measured is in normal state.
In step 2), the drying can use blotting paper fully to dry abalone shell.
In step 4), the temperature of the seawater can be 16~24 DEG C, it is described put back in clean seawater recovered when
Between can be no less than 10min, the speed of the heating treatment can be 1 DEG C/(5~30) min, and the program of heating treatment can be in the heart of Bao
Rate stops when being less than 20~40 times/min, to ensure that tested sample can survive after evaluation and test terminates；The heart rate data that is obtained with
Temperature data should all carry out corresponding processing conversion, to meet the needs of Arrhenius equations.
The present invention has advantages below：
1) entirely experiment evaluation and test can be completed indoors, and condition is controllable, avoids the various emergency cases in natural waterses；
2) entirely the test and appraisal cycle is shorter, and the assessment work of a colony can be completed in 10 days, save it is a large amount of artificial and
Bait cost.
3) tested and assessed in more traditional laboratory, this method focuses on the change of Bao heart rate, real not using the survival of Bao as index
After testing, sample can survive, meanwhile, this method is in units of individual, and the two advantages to screen the excellent Bao of heat resistance
Individual is possibly realized, so as to provide huge benefit for breeding work.
Brief description of the drawings
Fig. 1 is the Arrhenius curve maps of Bao heart rate of the embodiment of the present invention.
Fig. 2 is the heart rate ABT values of Bao not of the same race of the embodiment of the present invention.In fig. 2, mark zero is haliotis discus hannai Ino；Δ is west
Family name Bao；Is western disk Bao.
Fig. 3 is the heart rate ABT values of haliotis discus hannai Ino different groups of the embodiment of the present invention.
Fig. 4 is the heart rate ABT values of haliotis discus hannai Ino difference family of the embodiment of the present invention.
Embodiment
Following examples will the present invention is further illustrated with reference to accompanying drawing.
The evaluating method of Bao heat resistance described in the invention is by the monitoring to Bao heart rate under condition of different temperatures
Realize, infrared sensor is adhered on shell, the surface of Bao cardiac position, then using the mode of water-bath, gradually changed
The temperature of Bao local environment, the situation of Bao changes in heart rate is recorded in real time, then by the fitting of Bao heart rate and relevant temperature data,
Calculate the ABT values of Bao heart rate, the pointer as Bao temperature tolerance.
As shown in the Arrhenius curves (referring to Fig. 1) of Bao heart rate, with the rise of water temperature, the heart rate of Bao, which is presented, first to be risen
The trend reduced rapidly after height, the heart rate of Bao is fitted with corresponding temperature respectively before and after flex point, two equation intersection points
Abscissa is required ABT values.
Specific embodiment given below.
Fig. 2 provides the heart rate ABT values of Bao not of the same race of the embodiment of the present invention.Fig. 3 provides haliotis discus hannai Ino of the embodiment of the present invention not
With the heart rate ABT values of colony.Fig. 4 provides the heart rate ABT values of haliotis discus hannai Ino difference family of the embodiment of the present invention.
Embodiment 1
Haliotis discus hannai Ino, Xi Shi Baos and western disk Bao Sanzhe ABT value significant differences (F(2,26)=10.861, p<0.001), its
The ABT values of middle haliotis discus hannai Ino are 30.00 ± 1.23 DEG C, and the ABT values of Xi Shi Baos have been higher by 1.3 DEG C of (p compared with haliotis discus hannai Ino<0.05),
The ABT values of western disk Bao improve 2.5 DEG C of (p compared with haliotis discus hannai Ino<0.01), this evaluating result is consistent with actual cultivation situation.Table
Bright heart rate ABT assay method can be used for distinguishing the heat tolerance difference between these three Baos.
Embodiment 2
Heat tolerance difference (the p between haliotis discus hannai Ino different breeding colony can be distinguished using the assay method of heart rate ABT values
<0.05), wherein, the heat tolerance of Yang Xia colonies (YX) is most strong, is significantly higher than Dongshan (DS), Japanese (RB) and red shell color
(Red) colony.
Embodiment 3
Heart rate ABT assay method is similarly applied to the screening operation of haliotis discus hannai Ino difference family heat tolerance difference
In.As shown above, the heart rate ABT values of the family of haliotis discus hannai Ino 201 are minimum, reach the level of signifiance (p with the difference of 245 familys<
0.05)。
Claims (7)
1. a kind of quick assay method for distinguishing Bao heat resistance, it is characterised in that comprise the following steps：
1) abalone sample to be measured is temporarily supported in circulation, feeds fresh fragrant plant mentioned in ancient texts or sea-tangle, clear up residual bait and excrement in time,
Feed；
2) by abalone sample to be measured, the attachment on its shell is removed, abalone shell is dried；
3) method that the heart rate measurement of Bao uses non-invasive, the accurate location of Bao heart is sought using infrared sensor, makes letter
Number intensity is not less than 1V, and infrared sensor is fixed on Bao shell using glue after determining position；
4) Bao for fixing infrared sensor is put back in clean seawater and recovered, is lost in operation for supplementing Bao
Moisture, then using water-bath, heating treatment is carried out with fixed rate, until Bao heart rate is remarkably decreased, stops heating, Bao is turned
Move in normal seawater, in continuous mode, record the change of Bao heart rate and corresponding temperature in real time；
5) heart rate of acquisition is handled with temperature data, is then that the longitudinal axis draws Arrhenius using temperature as transverse axis, heart rate
Curve, linear regression is carried out to two sections of curves respectively before and after Arrhenius points of inflexion on a curve, obtains corresponding regression equation,
Two equations of simultaneous, the abscissa of intersection point is the heart rate ABT values of Bao；
6) in each tested colony, no less than 20 healthy individuals is randomly selected and are used to determine, averaged every for characterizing
The heat tolerance of individual colony.
A kind of 2. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 1), institute
It is 16~25 DEG C to state the temperature in circulation, and salinity is 30 ‰~35 ‰, and concentration is not less than 6mg/L.
A kind of 3. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 1), institute
Nursing time is stated as 5~15 days.
A kind of 4. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 2), institute
State to dry and fully dried abalone shell using blotting paper.
A kind of 5. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 4), institute
The temperature for stating seawater is 16~24 DEG C.
A kind of 6. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 4), institute
State and put back in clean seawater the time recovered and be no less than 10min.
A kind of 7. quick assay method for distinguishing Bao heat resistance as claimed in claim 1, it is characterised in that in step 4), institute
The speed for stating heating treatment is 1 DEG C/(5~30) min, and the program of heating treatment is stopped when the heart rate of Bao is less than 20~40 times/min
Only.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110169379A (en) * | 2019-07-02 | 2019-08-27 | 漳州鹏丰水产科技有限公司 | A kind of high-efficiency breeding method of the good high-quality abalone fry of environmental suitability |
| CN112070401A (en) * | 2020-09-10 | 2020-12-11 | 中国海洋大学 | Novel rapid determination method for temperature resistance index ABT of bay scallop |
| CN113661956A (en) * | 2021-07-16 | 2021-11-19 | 厦门大学 | Rapid evaluation method for high-throughput abalone high-temperature resistance |
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| US20050011470A1 (en) * | 2002-11-08 | 2005-01-20 | Dataflow/Alaska, Inc. | System for uniquely identifying subjects from a target population |
| EP1499179B1 (en) * | 2002-05-02 | 2007-12-19 | Sintef Fiskeri Og Havbruk AS | Sliding bulkhead for sorting of live fish |
| CN105165675A (en) * | 2015-08-21 | 2015-12-23 | 中国海洋大学 | Method using heartbeat as breeding index of parent scallops for bay scallops |
| CN105191837A (en) * | 2015-08-21 | 2015-12-30 | 中国海洋大学 | Heartbeat index based method for rapidly assessing scallop resistance |
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| EP1499179B1 (en) * | 2002-05-02 | 2007-12-19 | Sintef Fiskeri Og Havbruk AS | Sliding bulkhead for sorting of live fish |
| US20050011470A1 (en) * | 2002-11-08 | 2005-01-20 | Dataflow/Alaska, Inc. | System for uniquely identifying subjects from a target population |
| CN105165675A (en) * | 2015-08-21 | 2015-12-23 | 中国海洋大学 | Method using heartbeat as breeding index of parent scallops for bay scallops |
| CN105191837A (en) * | 2015-08-21 | 2015-12-30 | 中国海洋大学 | Heartbeat index based method for rapidly assessing scallop resistance |
| CN105191836A (en) * | 2015-08-21 | 2015-12-30 | 中国海洋大学 | Scallop heartbeat index based rapid seed selection method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110169379A (en) * | 2019-07-02 | 2019-08-27 | 漳州鹏丰水产科技有限公司 | A kind of high-efficiency breeding method of the good high-quality abalone fry of environmental suitability |
| CN112070401A (en) * | 2020-09-10 | 2020-12-11 | 中国海洋大学 | Novel rapid determination method for temperature resistance index ABT of bay scallop |
| CN113661956A (en) * | 2021-07-16 | 2021-11-19 | 厦门大学 | Rapid evaluation method for high-throughput abalone high-temperature resistance |
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Application publication date: 20171124 |