CN101731167A - Marking method of selective breeding individual of turbot - Google Patents
Marking method of selective breeding individual of turbot Download PDFInfo
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- CN101731167A CN101731167A CN200910255819A CN200910255819A CN101731167A CN 101731167 A CN101731167 A CN 101731167A CN 200910255819 A CN200910255819 A CN 200910255819A CN 200910255819 A CN200910255819 A CN 200910255819A CN 101731167 A CN101731167 A CN 101731167A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a marking method of a selective breeding individual of turbot. The method comprises the following steps of: firstly, selecting a glue needle, a hangtag gun and a mark board, wherein the glue needle is 10mm and in a transverse H shape, the model number of the glue needle is MUXP-25, the model number of the hangtag gun is SAGA 70L, the mark board is made of Solisep white polyester composite paper, the sign board has a length of 2 centimeters and a width of 0.8 centimeter, and a mark code is a 5-bit identifier; secondly, selecting a turbot juvenile fish the body length of which is longer than 5 centimeters, and keeping the fish to have a swimming posture during marking; positioning an inserting point at a part having more muscles behind the left eye on the head of the fish body; reserving a length of 2-8 millimeters of the glue needle in vitro; and finally, putting the marked fish in a 10ppm florfenicol medicated bath for 2 hours, carrying out the medicated bath once each day and continuously carrying out the medicated bath for three days. The method has simple operation, low cost, high automation degree and smaller damage to the fish body. The retention rate of a mark is higher than 90 percent, and the effective recognition rate is higher than 90 percent. The normal activity of the turbot cannot be influenced, and the method can meet the requirements of the large-scale breeding and marking of the turbot.
Description
Technical field:
The invention belongs in fish breeding, population estimation, stock assessment and migration and distribution investigation field Mesichthyes individual mark technology, is that a kind of big quantity of identification that realizes in the turbot breeding detects the technical method of each bar fish.
Background technology:
Turbot (Scophthalmus maximus L.) is northeast, the Atlantic Ocean, the coastal distinctive a kind of flatfish in Europe.China introduced the turbot seed from Britain first in 1992, because the parent population of adopting in China's turbot seed production process is usually without the science seed selection, for many generations culture and inbreeding in addition, cause the germplasm degradation phenomena of offspring seed more serious, occurred that incubation rate and emergence rate reduce, proterties degradation phenomenas such as growth rate slows down, resistance variation, needed to carry out the selection breeding work of turbot for this reason.In the breeding of turbot, can follow the trail of the dispatching from the factory and sell situation such as whereabouts of colony source, father and mother's basis, pedigree, production performance and fish of every fish by mark, simultaneously when carrying out the detection relevant with economic characters, need with separate sources, in same environment until the turbot young of forming, be implemented in the detection under the same breeding environment, so that improve the accuracy that genetic parameter is estimated.' based on a kind of parent fish marking method of turbot selective breeding ' (application number: 200810083837.1) of application such as the Ma Aijun of Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science, be that material is made reference numerals and put into liquid nitrogen container and left standstill 3 minutes mainly, be placed on fish body tag position after the taking-up 2 minutes with red metal; Expense required for the present invention is low, easy and simple to handle, and effect significantly and be easy to observe; But this patent also requires to arrive certain laggard row labels of specification at fish length, and because the turbot growth is fast, it is high-visible to be difficult to assurance time length back reference numerals, thereby reduces effective discrimination of mark; And red metal to be that material is made reference numerals (the reference numerals difference that each is individual, thereby need the red metal number of molds of tape label numeral very big) cost higher.(application number: 200810018667.9), wherein the individual marking method of big quantity mainly is the PIT tagging chip to be injected into carry out individual mark in the fish body to the patent " a kind of method of marking tilapia individual " that " the fish tag Study on Technology progress " of the Zhang Tanglin of Inst. of Hydrobiology, Chinese Academy of Sciences etc. (Chinese aquatic science/2003 year/the 10th/the 3 phase of volume) and the Dong of China Aquatic Science Research Academy Fresh Water Fishery Research Center apply for outstanding person etc.; But be subjected to the influence of PIT tagging chip size, this labeling method requires at fish length to certain specification (generally being applied to the mark of fish adult); And the higher (price: a PIT tagging chip, homemade about 20 yuans of PIT tagging chip existing market price; External import is more than 30 yuans), cost is high too undoubtedly for the seed selection breeding of extensive turbot.
Summary of the invention:
The objective of the invention is: in the breeding of turbot, colony source, father and mother's basis, pedigree, the production of following the trail of every fish by mark shows and the dispatching from the factory and sell situation such as whereabouts of fish, simultaneously when carrying out the detection relevant with economic characters, the Different Individual and the family of raising together with are discerned, improve the accuracy that genetic parameter is estimated, the parent that reserves seed for planting for fish provides identity marks simultaneously, realizes turbot mating controllability, avoids inbreeding depression.
The technology used in the present invention method comprises: 1, the preparation before the mark; 2, the selection of fish; 3, the mark of fish body.
1, the preparation before the mark: choose plastic pin, mark card hanging gun and Sign Board, plastic pin is 10mm, tack, be " worker " font, model is: MUXP-25, prepare the gas stone of wooden dipper net, towel, the pond that fresh seawater is housed and inflation simultaneously, and be that 75% alcohol carries out alcohol disinfecting to mark card hanging gun, plastic pin, Sign Board, hand with concentration;
2, the selection of fish: turbot is the live body juvenile fish, and body is grown up in 5cm; Before the mark, turbot must be supported 1-2 days in first pond, and juvenile fish can not be taken food; During mark with wet towel with the whole embedding of its head;
3, fish body tag: before inserting the fish body with mark card hanging gun rifle pin, smear sterilization rifle pin with Mupirocin Ointment or erythromycin ointment, Sign Board is nested on the rifle pin then, the mark card hanging gun model is: SAGA 70L; When mark card hanging gun rifle pin inserts the fish body, plastic pin is penetrated in the muscle in the fish body, the insertion point at fish body head (head is towards operating personnel) left eye rear side muscle than many places, pin will carefully penetrate muscular tissue, do not run into bone or vein, smear wound, the plastic pin of threading a needle with Mupirocin Ointment or erythromycin ointment and should leave length 2～8mm, adjust mark and make it be the oblique fish body of miter angle rear end, the fish of mark is placed in the seawater pond of charger for the fish bulk-growth; The Florfenicol dipping is 2 hours behind the mark, dipping every day once, continuously dipping is three days.Florfenicol dipping concentration is 10ppm.
Be labeled as Sign Board: material is a shellfish enlightening board white polyester extrusion coating paper, and double-deck overlay film is shaped as: long 2 centimetres, wide 0.8 centimetre, rope hole directly is 3.0 millimeter; Printing identification code on the white polyester extrusion coating paper, is 5 identifier: first bit identifier is an English capitalization, and the 2-5 position is Arabic numerals, as: A0001-A9999.
Characteristics of the present invention are:
1, the present invention is simple to operate, and the automation height is less to the degree of injury of fish body, and the retention rate of mark is more than 90%, and effectively discrimination does not influence the normal activity of turbot more than 90%, can satisfy the needs of the seed selection breeding mark of extensive turbot;
2, the present invention can mark to the fish young and adult, and mark does not have influence to the growth of turbot;
3, used turbot individual mark cost is very low, and the cost of every fish is 0.2 yuan, and the fish that detects only needs at the larval stage mark once, can carry all the life, has both saved cost, has also reduced repeatedly mark to the injury of fish body.
Description of drawings:
Fig. 1: fish Sign Board schematic diagram;
Fig. 2: fish labeling method and process:
A: mark card hanging gun rifle pin is inserted muscular tissue;
B: adjust mark and make it be the oblique fish body of miter angle rear end.
Embodiment:
In conjunction with the accompanying drawings the technology of the present invention is described in detail below by example:
This experiment is carried out in Shandong Province Huanghai Aquatic Products Co.,Ltd., Haiyang City precious marine product field, and its technical method of the present invention comprises: 1, the preparation before the mark; 2, the selection of young turbot; 3, the mark of seedling body.
1, the preparation before the mark:
Choose a kind of little, tack, the plastic pin (10mm, model: MUXP-25, material are polyethylene) that is " worker " font, the mark card hanging gun (model: SAGA 70L) of the pin that carries a gun.The Sign Board that uses: material is shellfish enlightening board white polyester extrusion coating paper, and double-deck overlay film is shaped as: long 2 centimetres, wide 0.8 centimetre, rope hole directly is 3.0 millimeter.Printing identification code on the white polyester extrusion coating paper, is 5 identifier:: first bit identifier is an English capitalization, and the 2-5 position is Arabic numerals, as: A0001-A9999, see accompanying drawing 1.Prepare the gas stone of wooden dipper net, towel, the pond that fresh seawater is housed and inflation simultaneously, and mark card hanging gun, plastic pin, Sign Board are carried out disinfection with 75% alcohol.
2, the selection of young turbot:
Get 608 of the close turbot family live body juvenile fish of specification, body length about body weight 170g, is wherein prepared marks for 304 about 16cm, supports several days temporarily earlier cultivating the pond, makes the test fish adapt to growing environment behind the mark; Before the mark fish of supporting temporarily in the pond was stopped eating two days; For reduce and eliminate in labeling process fish body suffered coerce and owing to coerce and cause the too damage that causes of strenuous exercise of fish body, turbot must be before mark with towel with the whole embedding anesthesia of its head, afterwards juvenile fish is carried out mark.。
3, the mark of seedling body:
Under same breeding environment, fish are detected accurately, need the fish body itself to have a kind of distinctive identification marking.Fish body identification marking is more, comprises additives, insert, or the outside is injected into the fish body.This method has adopted external individual marking method of listing in turbot class breeding technique, discern each tail in the big quantity detection fish uniquely.
Concrete mark program is:
1), make fry keep the posture of swimming;
2), the insertion point at fish body head (head is towards operating personnel) left eye rear side muscle than many places;
3), before inserting the fish body with mark card hanging gun rifle pin, Mupirocin Ointment (or erythromycin ointment) is smeared sterilization rifle pin, Sign Board is nested on the rifle pin then;
4), mark card hanging gun rifle pin is inserted under the scale, pin will carefully insert muscular tissue, does not run into bone or vein, sees the A figure of accompanying drawing 2;
5), directly plastic pin is inserted in the fish body muscle, smear the wound of sterilizing after threading a needle with Mupirocin Ointment (or erythromycin ointment);
6), that plastic pin should leave 5mm is long, for the fish bulk-growth.The length of its plastic pin is to decide the length of reserving plastic pin according to the maximum growth of the fish body that is supposed to (promptly determining according to the age or the weight of results);
7), adjust mark and make it be the oblique fish body of miter angle rear end, see the B figure of accompanying drawing 2, and at once the fry of mark be placed in the seawater pond of charger;
8), behind the mark with Florfenicol 10ppm (10% water-solubility florfenicol powder) dipping 2 hours, dipping every day once, dipping is three days continuously.
Mark group and control group (unmarked) are each 304, are placed in the same pond and culture, and measure its body length, body weight growth indexes after 30 days and 90 days in initial, breed respectively, the parameters behind the statistics mark, and measure the long characteristic index of all sashimi (raw fish).
During whole mark fish and unmarked fish culture, in 304 tail tags note turbot, the survival rate of fish is 98.03%, and not infecting proportion is 96.05%, and the conservation rate of mark is 94.08%, effectively discrimination 100%.The turbot of mark group and control group is initial, the average body of 30 days and 90 days is long and body weight is as shown in table 1.The result of one-way analysis of variance shows that the turbot of mark group and control group is in 30,90 days growth, and there are not significant difference in body length and body weight.
Long and the body weight (mean+SD) of the body of table 1 mark turbot and control group
Can find out that from above-mentioned table mark fish and unmarked fish be in the variation of growth traits such as average weight, average body are long indifference almost, thereby by mark and the growth contrast of mark fish are not found that mark does not have influence to the growth of turbot.
2007 and 2008 have been carried out large-scale application in Shandong Province Huanghai Aquatic Products Co.,Ltd., Haiyang City choice seafood aquiculture field to the inventive method; annual mark turbot seed is more than 20,000; the retention rate of mark is more than 90%; effectively discrimination is more than 90%, and do not influence the normal activity of turbot.Show that simultaneously this invention is simple to operate, the automation height, less to the degree of injury of fish body, can satisfy the needs of extensive turbot breeding mark.
Claims (2)
1), the preparation before the mark 1. marking method of selective breeding individual of turbot comprises:; 2), the selection of fish; 3), the mark of fish body, it is characterized in that:
1), the preparation before the mark: choose plastic pin, mark card hanging gun and Sign Board, plastic pin is 10mm, tack, be " worker " font, model is: MUXP-25, prepare the gas stone of wooden dipper net, towel, the pond that fresh seawater is housed and inflation simultaneously, and be that 75% alcohol carries out alcohol disinfecting to mark card hanging gun, plastic pin, Sign Board, hand with concentration;
2), the selection of fish: turbot is the live body juvenile fish, and body is grown up in 5cm; Before the mark, turbot must be supported 1-2 days in first pond, and juvenile fish can not be taken food; During mark with wet towel with the whole embedding of its head;
3), fish body tag: before inserting the fish body with mark card hanging gun rifle pin, smear sterilization rifle pin with Mupirocin Ointment or erythromycin ointment, Sign Board is nested on the rifle pin then, the mark card hanging gun model is: SAGA 70L; When mark card hanging gun rifle pin inserts the fish body, plastic pin is penetrated in the muscle in the fish body, the insertion point at fish body head left eye rear side muscle than many places, pin will carefully penetrate muscular tissue, do not run into bone or vein, smear wound, the plastic pin of threading a needle with Mupirocin Ointment or erythromycin ointment and should leave length 2～8mm, adjust mark and make it be the oblique fish body of miter angle rear end, the fish of mark is placed in the seawater pond of charger for the fish bulk-growth; The Florfenicol dipping is 2 hours behind the mark, dipping every day once, continuously dipping is three days; Florfenicol dipping concentration is 10ppm.
2. a kind of marking method of selective breeding individual of turbot according to claim 1, it is characterized in that described Sign Board: material is a shellfish enlightening board white polyester extrusion coating paper, and double-deck overlay film is shaped as: long 2 centimetres, wide 0.8 centimetre, rope hole directly is 3.0 millimeter; Printing identification code on the white polyester extrusion coating paper, is 5 identifier: first bit identifier is an English capitalization, and the 2-5 position is Arabic numerals, as: A0001-A9999.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910255819A CN101731167A (en) | 2009-12-28 | 2009-12-28 | Marking method of selective breeding individual of turbot |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910255819A CN101731167A (en) | 2009-12-28 | 2009-12-28 | Marking method of selective breeding individual of turbot |
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| CN200910255819A Pending CN101731167A (en) | 2009-12-28 | 2009-12-28 | Marking method of selective breeding individual of turbot |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475189A (en) * | 2015-12-03 | 2016-04-13 | 大连海洋大学 | Individual marking method applied in echinoderm breeding process achieved through BLUP method |
| CN105532529A (en) * | 2015-12-23 | 2016-05-04 | 孙欣 | Shellfish pedigree breeding marking contrast method |
| CN112868585A (en) * | 2021-01-26 | 2021-06-01 | 浙江省淡水水产研究所 | Method suitable for large-scale breeding individual marking of red swamp crayfish families |
-
2009
- 2009-12-28 CN CN200910255819A patent/CN101731167A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105475189A (en) * | 2015-12-03 | 2016-04-13 | 大连海洋大学 | Individual marking method applied in echinoderm breeding process achieved through BLUP method |
| CN105532529A (en) * | 2015-12-23 | 2016-05-04 | 孙欣 | Shellfish pedigree breeding marking contrast method |
| CN112868585A (en) * | 2021-01-26 | 2021-06-01 | 浙江省淡水水产研究所 | Method suitable for large-scale breeding individual marking of red swamp crayfish families |
| CN112868585B (en) * | 2021-01-26 | 2023-01-03 | 浙江省淡水水产研究所 | Method suitable for large-scale breeding individual marking of red crayfish families |
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Open date: 20100616 |