CN108142342B - Method for marking individual crabs by using electronic tag - Google Patents
Method for marking individual crabs by using electronic tag Download PDFInfo
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- CN108142342B CN108142342B CN201711424029.2A CN201711424029A CN108142342B CN 108142342 B CN108142342 B CN 108142342B CN 201711424029 A CN201711424029 A CN 201711424029A CN 108142342 B CN108142342 B CN 108142342B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/90—Sorting, grading, counting or marking live aquatic animals, e.g. sex determination
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K17/00—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations
- G06K17/0022—Methods or arrangements for effecting co-operative working between equipments covered by two or more of main groups G06K1/00 - G06K15/00, e.g. automatic card files incorporating conveying and reading operations arrangements or provisious for transferring data to distant stations, e.g. from a sensing device
Abstract
The invention discloses a method for marking crab individuals by using an electronic tag. The method of the invention can reliably and rapidly identify individual crabs, obviously reduce the identification cost, and can be applied to a plurality of stages of crab growth and development.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for individually marking crab living bodies by using a miniature radio frequency tag and managing aquaculture before and after marking.
Background
River crabs, swimming crabs, blue crabs and other crabs are the economic crabs mainly cultured in China. The crabs have wide breeding places in inland and coastal areas in China and the industrial production value is increased year by year. In the fields of crab genetic breeding, proliferation and releasing, and the like, individual marking needs to be carried out on partial crabs. The existing marking technology comprises physical marking, morphological marking, molecular marking and the like, and has some disadvantages: (1) the conventional physical marks comprise injection fluorescent gel, sticking labels, hanging labels, line codes and the like, the injection gel can be only used when the shells of individual crabs are relatively transparent in the early growth stage, and the individual can be marked only when the number of the marked combinations is limited; the sticking label and the hang tag can only mark the adult of which the adult does not exuviate, because the crabs belong to crustaceans, the growth of body length and weight is realized by the exuviation mode, and the external mark is easy to lose along with exuviation or limb breakage to cause the failure of marking individuals after the exuviation for many times in the life cycle; the line code mark can be used for batch marking, and is suitable for group marking which can not effectively distinguish living bodies and individuals. (2) Morphological marking, such as marking by cutting off a portion of the appendage or cutting the appendage into a particular shape, marks crabs in a limited number and can regenerate after exuviation and be difficult to distinguish. (3) The molecular marker needs expensive molecular biological equipment and biopsy taking for detection, is time-consuming and high in cost for distinguishing, cannot detect in real time and can only distinguish families or groups. At present, a plurality of research institutions, production units and distribution enterprises have no convenient and effective marking way for individual marking of living crabs. For example, breeding and breeding still need to be carried out in separate ponds, and the defects of large influence on environmental effect evaluation, low water utilization rate and high management cost exist. The electronic tag is applied to fishes, amphibious reptiles and some land animals, and because the circulating systems of the electronic tag are closed-tube type circulation, the tag implanted wound can heal quickly, and the survival state of the electronic tag cannot be seriously influenced due to excessive blood loss. The crabs are open-tube circulatory systems, important tissues and organs such as blood sinuses, hearts, thoracic nerve groups and the like are easy to be punctured and damaged by improper marker implantation to cause life threat, in addition, the daily movement and molting activity of the crabs depend on a closed 'hydraulic' system, wounds implanted by the labels are not easy to heal quickly like other animals, and in addition, bleeding causes 'hydraulic' pressure reduction to influence the movement and even the molting of the crabs. Therefore, the appropriate position and angle must be explored to minimize the risk of implanting the tag.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a method for marking the living bodies of crab individuals by using an electronic tag. The method can make the marked living crabs reach the individual distinguishing level on the premise of relative reliability and lower practical application cost, is not influenced by the growth and development of molting, and can identify the marked individual of each crab in real time at each growth and development stage after marking. In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
a method for marking individual crabs by using an electronic tag comprises the step of placing the electronic tag in a muscle box on the lateral side of a cephalothorax through a relatively weak part between the side steps and feet of the crabs.
The relative weakness between the side feet of the crab is the position between the chela on the same side of the crab and the first step foot, between the first step foot and the second step or between the third step foot and the fourth step foot.
In another preferred embodiment of the invention, the crab is not a freshly molted "soft shell crab" and the individual about to molt.
In another preferred embodiment of the present invention, the crab-type marker is one having a body weight of more than 1.5g, more preferably 3.0g to 5.0 g.
In another preferred embodiment of the present invention, the crabs are washed in circulating or running water and stopped eating for 1 day before the electronic tag is implanted, and they are sterilized in a container of sterilizing solution for 5 minutes to 10 minutes and the injection site is sterilized with 75% alcohol at the time of implanting the electronic tag.
In another more preferred embodiment of the invention, the disinfecting solution is diluted with 10% povidone-iodine disinfectant for use as specified.
In another preferred embodiment of the invention, after the electronic tag is implanted, the crabs are put into a sterilized water body for temporary rearing for more than 24 hours.
In another more preferred embodiment of the present invention, the crabs may be exposed to the water for 30 minutes to 1 hour before being put into the sterilized water body after the electronic tags are implanted for temporary rearing.
In another preferred embodiment of the invention, the crabs are fed a little after 24 hours after the implantation of the electronic tag.
In another preferred embodiment of the invention, the dissolved oxygen in the crab culture environment after the electronic tag is implanted is more than 5mg/L, and the water quality is stable.
In another preferred embodiment of the invention, the electronic tag is a radio frequency tag (RFID) encapsulated by a tube of electronic marker material coated with a slip-resistant biological coating on the outside of the tube.
In another preferred embodiment of the present invention, a method for marking crab individuals by using an electronic tag comprises the following steps:
1) providing an electronic tag, and selecting the specification of the electronic tag to be adaptive to the specification of the crab to be marked;
2) providing the crabs after the molting peak, and concentrating the crabs in circulating water or flowing water for flushing and stopping eating for 1 day;
3) fishing the crabs in the step 2) into a container containing disinfectant for disinfection for 5-10 minutes, disinfecting an injection part by using 75% alcohol cotton, implanting electronic tags between the chela and the first step feet, between the first step feet and the second step or between the third step feet and the fourth step feet on the same side of the crabs, and placing the crabs in a muscle box on the side surface of the cephalothorax.
4) After the electronic tag is implanted, the crab which is resistant to the exposure can be exposed for 30 minutes to 1 hour according to the environmental temperature and then put into a sterilized water body for temporary culture; and directly putting the crabs which are not resistant to the dry dew into a sterilized water body for temporary culture, and feeding a small amount of the crabs after 24 hours.
The method of the invention utilizes the micro electronic tag to implant the proper position in the crab body with certain specification for marking and then polyculture in the same pool, thus solving the problem that the prior art has no convenient and effective marking mode for individual marking of the crab living body, because the electronic tag can carry out more than hundreds of millions of nonrepetitive digital string codes, can carry out editing and encryption according to the definition rule, realizes mass individual marking, and can quickly respond and identify the specific individual code even in the living crab forming body due to good mark stress resistance and sensitive response. The method of the invention has the following outstanding advantages:
(1) the individual identification reliability and speed of the crabs are greatly improved, the identification cost is obviously reduced, and the method can be applied to a plurality of stages of crab growth and development.
(2) Under the condition of basically not influencing the final culture survival rate of the cultured marked crabs, the breeding of the selected crabs and the breeding of the control crabs can be realized in the same pool as early as possible, the number of ponds required by single culture and the culture management cost are greatly reduced, data before and after the growth and development of each crab can be obtained, and the reliability and the breeding efficiency of crab breeding evaluation are improved.
(3) The application range is wide: besides crab breeding application, batch code scanning identification can be realized for recaptured crabs during propagation and releasing evaluation, and the recapture rate can be rapidly counted and the releasing individual distribution, growth and development and other conditions can be evaluated according to the marked individual proportion.
Drawings
Fig. 1 shows reference patterns and specifications of an implanted electronic tag.
Fig. 2 is a schematic diagram of the part of the crab where the electronic tag is implanted, wherein 1 represents an injection part 1, 2 represents an injection part 2, 3 represents an injection part 3, 4 represents an indwelling position 1, 5 represents an indwelling position 2, and 6 represents an indwelling position 3.
Detailed Description
Aiming at the problem that a convenient and effective marking mode for individually marking crab living bodies does not exist in the prior art, the inventor of the application carries out intensive research, applies the electronic marking to the crab living body marking, and establishes a selection and implantation technology of a crab electronic tag and a breeding management method before and after marking. The method of the invention can reliably distinguish the living crab individuals with low cost. The present invention has been completed based on this finding.
The invention refers to the ability of tolerating the exposure in the air, that is, under the condition of proper temperature and humidity, the aquatic animals can leave the water body for a long time (more than 1 hour) without affecting the survival rate of the aquatic animals due to serious physiological stress caused by dryness, oxygen deficiency and the like.
Electronic tag selection
(1) The electronic mark material is mainly glass tube but not limited to radio frequency mark (RFID, style is shown in figure 1) packaged by glass tube, and biological coating can be added outside the tube to prevent slipping; (2) specification of the electronic tag: tags with different sizes are selected according to specifications of crabs to be marked, electronic tags with overlarge sizes are implanted to have adverse effects on survival and exuviation growth of the crabs, the more tiny tags have smaller influence, but the more tiny tags have higher price, and the marking specifications are reasonably selected according to the sizes of the crabs to be marked; (3) the electronic tag is a readable and writable chip, and can be read by a radio frequency reader, and the code information can be read by a read-write code integrated machine and can be rewritten, so that the electronic tag can be recycled, the marking cost is greatly reduced, the chip can be encrypted and locked, and the electronic tag can be safely applied to the fields of crab breeding, proliferation and releasing and the like; (4) the electronic label must be equipped with chip reader or reading coding information to distinguish individual, if self-coding, it must be equipped with read-write code integrated machine, and in the on-line computer software it can automatically rewrite electronic label information in batches according to serial number.
Marker crab selection
(1) The crabs to be implanted with the tags are healthy and strong in vitality, and the number of the appendage stumps is not more than 2; (2) the marked crabs can leave water for a short time without causing severe stress, and the 'soft-shell crabs' which just exuviate are not suitable to be selected, and the exuviation peak period of the crabs is avoided when large-scale label implantation is carried out in production; (3) the weight of the early marked crab larvae is more than 1.5g, 3.0 g-5.0 g is suitable, more than 10.0g is used for marking, and electronic tags with larger specifications can be used for further reducing the marking cost.
Electronic tag implantation mode
(1) An implantation part, wherein an electronic tag is implanted in a relatively weak part between the side and the feet of the crab by using a special syringe, so that the tag is left in a muscle box on the lateral surface of the cephalothorax (see figure 2); (2) the injection position can be between the chela and the first step foot (injection position 1), between the first step foot and the second step foot (injection position 2) or between the third step foot and the fourth step foot (injection position 3) according to the specification and the size of the crabs, and the injection can be performed at the left-right symmetrical positions; (3) the implantation depth and angle of the tag are as follows: the injection implantation depth should reach the body side muscle box (the remaining positions 1-3, the other side and the symmetrical positions) to prevent the molting and the falling out, and the implantation angle of the label should be careful to avoid injuring the substantive organs such as blood sinuses, abdominal nerve groups, heart, stomach and the like, otherwise, the marked crabs would die rapidly. The injection site of the implanted label and the indwelling site of the label are shown in figure 2. Before injection, the bulk electronic label should be soaked in 75% alcohol in advance for sterilization for more than 10 minutes, and the used injector should be used for multiple times and then used after timely sterilization. .
Electronic tag cultivation management before and after implantation
(1) Before the label is implanted: stopping eating the crabs for 1 day, and washing the crabs in circulating water or flowing water; if the temporary culture in still water is carried out, the sludge is flushed and then placed in a shallow water pool to keep aeration and change the water amount by more than 100 percent so as to clean the body surface and evacuate intestines and stomach, the temporary culture is carried out to avoid direct sunlight, and the environment temperature is preferably controlled between 10 ℃ and 25 ℃. Before the injection marking, fishing out the crabs to a container containing disinfectant (such as povidone iodine disinfectant, according to the indicated dosage) for disinfection for 5-10 minutes, properly inflating when the water level is high, and transferring the crabs to a waterless container after disinfection; the crabs which do not resist the dry dew can be transferred into the disinfected clean water body for standby, the crabs can be fished out and quickly kept well, and the injection part is wiped with 75 percent alcohol cotton for disinfection and then is implanted with a disinfection label. (2) After the label is implanted: and (3) drying the crab which is resistant to the dry dew for 30 minutes to 1 hour according to the ambient temperature after injection, and then putting the crab into the sterilized water body for temporary culture, wherein the crab which is not resistant to the dry dew is placed into the sterilized water body for temporary culture after the mark is implanted. After the label is implanted, dead individuals are removed in time by observing and observing within 24 hours, and the label is recovered for strict disinfection and then can be injected again for supplement; a small amount of feed can be fed after 24 hours, and the dissolved oxygen in the culture environment is kept above 5mg/L and the water quality is stable. And observing the marked crab groups for 48 to 96 hours, and then performing breeding arrangement according to the requirements. Before stocking, the marked crabs can be scanned one by one according to the marking order, and initial phenotype data information is measured and recorded.
The technical solution of the present invention will be further described with reference to specific examples, which should not be construed as limiting the technical solution of the present invention.
Example 1:
the individual marking of the river crab fries by using the electronic tag is carried out according to the following steps
1) Electronic tag selection: (1) the electronic mark material is a radio frequency mark packaged by a glass tube; (2) selecting crab seeds (crabs) with the specification of 12.8mm multiplied by 0.7mm, wherein the specification of the crabs to be marked is 3 g-5 g; (3) the electronic tag is a readable and writable chip, can be read by a radio frequency reader and uniformly coded by a code reading and writing integrated machine, and can clearly distinguish different groups and individuals, for example, "617181911110000-617181911110049" and "617181911210000-617181911210049" can respectively represent 50 male crabs in group 1 and 50 female crabs in group 1.
2) Marking crab selection: (1) the crabs to be implanted with the marks are healthy and have good vitality, and the number of the appendages and the stumps is not more than 2; (2) the marked crabs can leave water for a short time without causing severe stress, and the 'soft-shell crabs' which just exuviate and the individuals which are about to exuviate are removed; (3) the weight of the marked crabs is more than 1.5g and is mostly 3.0g to 5.0 g.
3) The electronic tag implantation mode comprises the following steps: (1) an injection position, wherein a miniature electronic tag is implanted in a relatively weak position (an injection position 1 and a symmetrical position thereof in figure 2) between the side chela and the first step leg of the crab by means of a special injector; (2) the implantation depth and angle of the tag are as follows: the injection implantation depth reaches the body side muscle box (the other side and the symmetrical position of the indwelling position 1), and the label implantation angle is slightly pushed to the back of the head, the chest and the nails of the crabs, so as to avoid injuring the abdominal nerve group and the heart and avoid puncturing and injuring the stomach of the crabs. (3) Adopting bulk electronic tags, soaking and disinfecting with 75% alcohol for 20 minutes before injection, placing each tag into 1.5mL centrifuge tubes filled with 75% alcohol according to sequence numbers before injection, arranging 50 centrifuge tubes in sequence, and facilitating operation; the injector should be soaked in alcohol for sterilization after each injection, and the sterilized label is placed after the alcohol cotton is wiped.
4) E, breeding management before and after the electronic tag is implanted: (1) before the label is implanted: stopping feeding the crabs for 1 day, washing mud, putting the crabs into a circulating water culture system, and temporarily culturing the crabs in the circulating water culture system; no direct sunlight exists, and the ambient temperature is about 18 ℃. Before marking the injection of the crabs, soaking the crabs for 10 minutes by 10 percent of povidone iodine disinfectant (0.05mL/L), transferring the crabs into a waterless container after disinfection, quickly fixing the crabs during injection, wiping the injection part by 75 percent of alcohol cotton, disinfecting, and implanting a disinfection label after injection. (2) After the label is implanted: after injection, the mixture is dried and exposed for 1 hour and then put into a sterilized water body for temporary culture. Dead individuals are observed and removed in time within 24 hours after the tags are implanted, and the tags are recovered and disinfected strictly, so that the injection can be performed again for supplement; a small amount of feed is fed after 24 hours, and the cultivation environment is kept sufficient in dissolved oxygen and stable in water quality. And carrying out single-box culture after observing the injected crab group for 48 to 96 hours. And (3) scanning the marked crabs one by one before stocking, measuring and recording initial phenotype data information, simultaneously performing single-breeding contrast with the same specification, and culturing until twice molting is completed.
By adopting the embodiment to carry out individual marking on the river crab seedlings (crabs) by utilizing the electronic tags, compared with the culture of a control group after single culture and 2 molting, the culture conditions are as follows:
TABLE 1 cultivation of electronically tagged and uninjected tagged crabs
The statistical culture data mean molting period and single molting weight gain rate can be known, and the difference of the growth data of the marker group and the control group is not obvious.
Example 2:
carrying out individual marking of the crabs and mixed culture of unmarked crabs in the same pool by using the electronic tags according to the following steps:
1) electronic tag selection: (1) the electronic mark material is a radio frequency mark packaged by a glass tube; (2) the specification of the crab seed selection electronic tag of which the specification is 3 g-5 g weight of the marked crabs is 12.8mm multiplied by 0.7 mm; (3) the electronic tag is a readable and writable chip, the recovery mark after the completion of the embodiment 1 is selected, and the recovery mark is recoded by using a read-write code integrated machine, so that different groups and individuals can be clearly distinguished, for example, "617181922110000-617181922110049" and "617181922210000-617181922210049" can respectively represent 50 female crabs and 50 female crabs in the same group.
2) Marking crab selection: (1) the crabs to be implanted with the marks are healthy and have good vitality, and the number of the appendages and the stumps is not more than 2; (2) the marked crabs can leave water for a short time without causing severe stress, and the 'soft-shell crabs' which just exuviate and the individuals which are about to exuviate are removed; (3) the weight of the marked crabs is more than 1.5g and is mostly 3.0g to 5.0 g.
3) The electronic tag implantation mode comprises the following steps: (1) an injection position, wherein a miniature electronic tag is implanted in a relatively weak position (an injection position 1 and a symmetrical position in figure 2) between the side chela and the first-step leg of the crab by a special injector; (2) the implantation depth and angle of the tag are as follows: the injection implantation depth reaches the body side muscle box (the other side and the symmetrical position of the indwelling position 1), the label implantation angle is slightly pushed to the back of the head, the chest and the nails of the crabs, so as to avoid injuring the abdominal nerve group and the heart and avoid puncturing and injuring the stomach of the crabs. (3) Adopting bulk electronic tags, soaking and disinfecting with 75% alcohol for 20 minutes before injection, placing each tag into 1.5mL centrifuge tubes filled with 75% alcohol according to sequence numbers before injection, arranging 50 centrifuge tubes in sequence, and facilitating operation; the used injector should be soaked in alcohol for sterilization after each injection, and the alcohol cotton is put into a sterilized label after wiping off excessive alcohol and residual hemolymph.
4) E, breeding management before and after the electronic tag is implanted: (1) before the label is implanted: stopping feeding the crabs for 1 day, washing mud, putting the crabs into a circulating water culture system, and temporarily culturing the crabs in the circulating water culture system; no direct sunlight exists, and the environmental temperature is about 20 ℃. Before marking the injection of the crabs, soaking the crabs in 0.05mL/L concentration povidone iodine (10%)' disinfectant for 10 minutes, transferring the crabs into a waterless container after disinfection, quickly keeping the crabs well during injection, wiping the injection part with 75% alcohol cotton, disinfecting, injecting and implanting a disinfected label. (2) After the label is implanted: after injection, the mixture is dried and exposed for 1 hour and then put into a sterilized water body for temporary culture. Dead individuals are observed and removed in time within 24 hours after the tags are implanted, and the tags are recovered and disinfected strictly, so that the injection can be performed again for supplement; a small amount of feed is fed after 24 hours, and the cultivation environment is kept sufficient in dissolved oxygen and stable in water quality. And (5) observing the crab injected population for 48 to 96 hours, and transferring the crab injected population into a polyculture pond. Before stocking, the marked crabs are scanned one by one, the initial phenotype data information is measured and recorded, the mixed breeding with the same quantity and specification is compared with the same pond stocking, the breeding density is 800 per 667 square meters, and the whole process is matched with feed for feeding.
When the electronic tags are used for individual marking of the river crab seedlings (crabs) in the above embodiment, compared with the control group cultivation, the cultivation condition and the individual differentiation cost of the grown crabs when being cultivated to be on the market or the individual differentiation cost of distributing the individuals to different families are as follows:
TABLE 2 electronic tags marking crab and contrast crab polyculture conditions
a, the contrast component adopts a kit method and a phenol-chlorine method respectively;
b, calculating the cost of each label of the patent marking group according to 5 times of repeated utilization and single use;
and c, the identification of the single body by fluorescence capillary electrophoresis and PAGE method is time-consuming.
The weight gain and survival rate of the marked crabs are slightly lower than those of control crabs, but the difference is not obvious, the development conditions of the marked crabs and the survival rate of the marked crabs are basically synchronous through anatomical observation, and the marked crabs are used as parents to breed without influence. The marking method of the present invention is significantly less expensive and time consuming for individual identification than other identification methods.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the present invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims and their equivalents.
Claims (10)
1. A method for marking individual crabs by using an electronic tag comprises the step of placing the electronic tag in a muscle box on the lateral side of a cephalothorax through a relatively weak part between the side steps and feet of the crabs.
2. The method of claim 1 wherein the relative weakness between the crab side footings is between the crab side chela and the first step foot, between the first step foot and the second step or between the third step foot and the fourth step foot.
3. The method of claim 1, wherein the crabs are not freshly molted "soft shell crabs" and individuals about to molt.
4. The method of claim 1, wherein the crab-type markers are crab-sized over 1.5 g.
5. The method according to claim 1, wherein the crabs are washed in circulating water or running water and stopped eating for 1 day before the electronic tag is implanted, and the injection part is sterilized with 75% alcohol for 5 to 10 minutes in a container of a sterilizing solution when the electronic tag is implanted.
6. The method of claim 1, wherein the crabs are placed in a sterilized water body for temporary rearing for more than 24 hours after the electronic tags are implanted.
7. The method of claim 6, wherein the crabs are allowed to dry for 30 minutes to 1 hour after being implanted with the electronic tag and before being placed in a sterilized water body for temporary rearing.
8. The method according to claim 6 or 7, wherein the crabs are fed a little after 24 hours of implanting the electronic tag.
9. The method according to claim 1, wherein the electronic tag is an electronic label material and is a radio frequency label packaged by a hard tube, and a biological coating for preventing slipping is coated outside the hard tube.
10. A method for individual marking of crabs using an electronic tag, comprising the steps of:
1) providing an electronic tag, and selecting the specification of the electronic tag to be adaptive to the specification of the crab to be marked;
2) providing the crabs after the molting peak, and concentrating the crabs in circulating water or flowing water for flushing and stopping eating for 1 day;
3) fishing the crabs in the step 2) into a container containing disinfectant for disinfection for 5-10 minutes, disinfecting an injection part by using 75% alcohol cotton, implanting electronic tags between the chela and the first step feet on the same side of the crabs, between the first step feet and the second step feet or between the third step feet and the fourth step feet, and placing the electronic tags in a muscle box on the side surface of the cephalothorax;
4) after the electronic tag is implanted, the crab which is resistant to the dew is dried and dew dried for 30 minutes to 1 hour according to the ambient temperature and then put into a sterilized water body for temporary culture; and directly putting the crabs which are not resistant to the dry dew into a sterilized water body for temporary culture, and feeding a small amount of the crabs after 24 hours.
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| CN101911921A (en) * | 2010-09-16 | 2010-12-15 | 厦门大学 | Individual fluorescence labeling method for early-stage newborn green crabs to adult green crabs |
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