CN103026998B - Externally-hung marker plate for artificial breeding of flatfish fry and marking method - Google Patents
Externally-hung marker plate for artificial breeding of flatfish fry and marking method Download PDFInfo
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- CN103026998B CN103026998B CN201310013644.XA CN201310013644A CN103026998B CN 103026998 B CN103026998 B CN 103026998B CN 201310013644 A CN201310013644 A CN 201310013644A CN 103026998 B CN103026998 B CN 103026998B
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- marker
- mark
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an externally-hung marker plate for artificial breeding of flatfish fry, which belongs to the technical field of culture and proliferation of marine biological resources. The externally-hung marker plate for the artificial breeding of the flatfish fry comprises an external marker end, a connecting line and an anchoring end, wherein the external marker end consists of a marker rod and an outer sleeve; encoding information is printed on the marker rod; the outer sleeve is tightly sleeved outside the marker rod; the marker end and the anchoring end are connected together by the connecting line; the connecting line is connected with one end of the marker end to form a straight line; and the other end of the connecting line is vertically connected with the anchoring end. The externally-hung marker plate for the artificial breeding of the flatfish fry is simple in structure, convenient to manufacture, light in weight and easy to operate; and the marker rod is coated by the transparent polypropylene outer sleeve and the outer sleeve is good in corrosion resistance, so that the information difficultly shed due to corrosion. The invention further provides a marking method used for the externally-hung marker plate for the artificial breeding of the flatfish fry. The externally-hung marker plate for the artificial breeding of the flatfish fry is anchored on the backs of the flatfish fry by using a special marking machine; the marked flatfish fry is high in survival rate; the plate-shedding rate is low; the externally-hung marker plate for the artificial breeding of the flatfish fry has small influence on the growth and the food intake amount of the marked flatfish fry; and the marking method is simple in operation and is applicable to marking of flatfish bodies on a large scale.
Description
Technical field
The invention belongs to living marine resources aquaculture technical field, relate to particularly a kind of flounder flounder class fingerlings artificial release sign board and the labeling method of external extension.
Background technology
In recent decades, the marine eco-environment that the day by day frequent and facing-port industry fast development of human social activity etc. brings is polluted constantly aggravation, cause China coastal seas living marine resources day by day to fail, marine ecosystems structure is increasingly fragile, and living marine resources quantity of the catch declines year by year.It is recover and supplementary living marine resources, maintain the main path of the ecological balance and bio-diversity that artificial fecundation is released, the biology of releasing is carried out to mark to be become and follows the trail of the only resource biological and assessment enhancement effect of releasing, and carries out the cultivation effect assessment of releasing and be country and fisheries management at different levels department and carry out the important evidence of fishery resources.At present, mainly comprise in body and two kinds of external tags for the denotation approach of marine fishes, wherein internal tag method complicated operation, dangerous high, detection need be by special instrument and equipment, economy and poor practicability.And external tag method simple to operate because having, observe directly perceived, economical and practical feature and widely apply, the mark of listing is denotation approach the most conventional in external tag technology.The general difficult drop-off of sign board, retention time is long, can be used for characterizing the fish of Different population, is easily found, and reclaims easy.
Flounder flounder class is that the master of the northern area of China marine fish culture supports kind, and wherein Cynoglossus semilaevis, lefteye flounder, halibut etc. are China's native country kind, are particularly suitable for stock enhancement and release.Flounder flounder class seed body thickness is less, height is narrower, and after metamorphosis completes, anophthalmia leans to one side to show consideration for the life of dwelling at the bottom of cultivating Chi Di battalion headquarter, and life habit is special.At present, conventional have anchor shape, ellipse, cylindrical board etc. for fresh water and seawater fish external tag board, is mainly used in the fingerling that mark specification is larger (the above fingerling of 20cm).In earlier stage, we easily penetrate fish body and cause compared with large wound and cause mortality in fish surface during for flounder flounder class small dimension seed (total length 7cm-11cm) mark, and de-board rate is higher.Meanwhile, because surface area and the weight of sign board are larger, affected the swimming behavior of mark fish, after having increased it and releasing at the probability of natural marine site prey.The external tagging research of listing of flounder flounder class of carrying out is both at home and abroad less, and has lethality and the high problem of de-board rate.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of for flounder flounder class fingerlings artificial release sign board and the labeling method of external extension, sign board of the present invention is anchored to flounder flounder class seed back, high, the de-board rate of mark fish survival rate is low, growth on mark fish and the impact of ingesting are little, and marking operation is simple, be labeled fish and be easy to identification, be applicable to the tagging of the flounder flounder classes such as Cynoglossus semilaevis, lefteye flounder, halibut.
The present invention realizes by following technical scheme:
A kind of for the release sign board of external extension of flounder flounder class fingerlings artificial, it is made up of external tag end, connecting line and anchored end, external tag end is by indicating that rod and outer tube form, on mark rod, be printed on coded message, outer tube is made up of transparent polypropylene material, and outer tube is closely wrapped in outside mark rod; Flag terminal and anchored end are linked together by connecting line, and connecting line is connected in alignment with one end of flag terminal, and the other end of connecting line is connected with anchored end is vertical;
Described sign board length 2.8-3.2cm, weight 0.02-0.034g;
Described mark rod length 1.2-1.6cm, weight 0.012-0.021g;
Described connecting line length 1.2-1.6cm;
Described anchored end length 0.6-0.8cm, anchored end diameter 0.5-0.8mm.
Further, described mark rod, connecting line and anchored end are all to be made by polyethylene material, safety non-toxic.
A kind of for the release labeling method of external extension sign board of flounder flounder class fingerlings artificial, concrete steps are as follows: be labeled fry and carry the previous day and stop eating, when marking operation, all fries all carry out dipping bath anesthesia with MS-222, marker uses after with 70% alcohol disinfecting, back, fish dorsal fin base portion below is selected to be labeled in the position of listing mark, marker and fish body are 45 ° of-60 ° of angles, after mark completes, extract fast gently marker out to point light pressure symbol location, sign board anchored end is anchored on fry health; After listing mark completes, mark fry continues to put back to indoor culture pond to be cultivated, and mark fish carries out soaking disinfection processing with 5mg/L terramycin, and mark fry is supported time 10-15d temporarily in indoor culture pond.
Described marker is the product of market sale and makes through transformation, comprises main frame, grappling ejection end, sign board holddown groove, striker, trigger five parts compositions.By the striker transformation of marker, diameter diminishes, and consistent with sign board anchored end diameter; Grappling is ejected to end and transform, by its reduced diameter, internal diameter is consistent with sign board anchored end diameter.In the time of listing operation, sign board is installed on to sign board holddown groove, cocking drives striker to touch sign board anchored end and sign board can be ejected fast by ejecting end.
The present invention's beneficial effect compared with prior art:
Sign board of the present invention is simple in structure, easy to make, and lightweight, marking operation is simple.Mark rod can mark the data messages such as problem title, numbering, and mark rod is outer coated with transparent polypropylene sleeve pipe, and sleeve pipe corrosion resistance and good ensures upper not perishable the coming off of information of mark rod.
The present invention is according to flounder flounder class body structure feature and life habit, development special mark board, be fixed on flounder flounder class seed back by special mark device, high, the de-board rate of mark fish survival rate is low, growth on mark fish and the impact of ingesting are little, marking operation is simple, is suitable for marking operation in enormous quantities.
The present invention adopts the special mark device mark of listing.Back, fish dorsal fin base portion below is selected to be labeled in the position of listing mark, and marker and fish body are 45 ° of-60 ° of angles, and sign board ejects and is anchored on fish body by marker grappling is most advanced and sophisticated, reaches the effect without wound mark.
By the flounder flounder class small dimension seed of sign board mark total length 7-9cm of the present invention, lefteye flounder seed is marked as motility rate and reaches more than 98%, and de-board rate is lower than 4%; The survival rate of labeling moiety smooth tongue sole offspring breed reaches more than 97%, and de-board rate is lower than 2%, and marking operation does not impact the growth that is labeled fish.As get rid of the accidental injury that manual operation brings, and the survival rate of mark fish can reach 100%, and mark effect is better.
Brief description of the drawings
Fig. 1: sign board structural representation of the present invention: 1, mark rod; 2, outer tube; 3, connecting line; 4, anchored end; A sign board length, B indicates excellent length, C connecting line length, D anchored end length, E anchored end diameter.
Embodiment
Narrate by reference to the accompanying drawings technology contents of the present invention below by two specific embodiments:
A kind of for the release sign board of external extension of flounder flounder class fingerlings artificial, as shown in Figure 1, it is made up of external tag end, connecting line 3 and anchored end 4, external tag end is by indicating that rod 1 and outer tube 2 form, on mark rod, be printed on coded message, outer tube is made up of transparent polypropylene material, and outer tube is closely wrapped in outside mark rod; Flag terminal and anchored end are linked together by connecting line, and connecting line is connected in alignment with one end of flag terminal, and the other end of connecting line is connected with anchored end is vertical;
Described sign board length A 2.8-3.2cm, weight 0.02-0.034g;
Described mark rod length B1.2-1.6cm, weight 0.012-0.021g;
Described connecting line length C 1.2-1.6cm;
Described anchored end length D0.6-0.8cm, anchored end diameter E0.5-0.8mm.
Further, described mark rod, connecting line and anchored end are all to be made by polyethylene material, safety non-toxic.
Embodiment mono-:
The 7-10 month in 2011 has been implemented the correlative study of sign board labeling moiety smooth tongue sole offspring breed of the present invention in Qingdao loyalty seawater product Co., Ltd, specific implementation process is as follows:
1, mark is selected with seed and is cultivated
Mark is the healthy seedling that the loyal seawater in Qingdao produces Co., Ltd's artificial culture with semi-smooth tongue sole offspring breed, and seed color and luster is normal, and size rule is neat, and bouncing is ingested good.
Select average length 8cm(body weight 3.2g-4.6g), 12cm(body weight 7.2g-9.7g), 16cm(body weight 18g-22g) seed for test.Utilize sign board of the present invention to carry out listing mark, sign board length is 2.8cm, weight 0.02g; Indicate excellent length 1.6cm, weight 0.012g; Connecting line length 1.2cm; Anchored end length 0.6cm, diameter 0.5mm.6 mark groups are set altogether, and every group uses seed 100 tails, and each mark group arranges a repetition.Mark is first 25m at volume with seed
3cement pit in support temporarily after 3d for test.Seed rearing condition: 21 DEG C-24 DEG C of water temperatures, salinity 26-29, pH7.8-8.2, more than dissolved oxygen 5mg/L.The nurturing period artifical compound feed of throwing something and feeding, feeding volume is the 2-3% of fish body weight, every day, cleaning was cultivated pond once.
2. flag operation method
Before on-test, all test fishes are carried the previous day and stop eating.Before flag operation, all experiment fishes all carry out dipping bath anesthesia with the MS-222 of 60mg/L.In the time of listing mark, carry out back listing mark operation with special mark device.Marker grappling tip is used after with 70% alcohol disinfecting.Back, fish dorsal fin base portion below is selected to be labeled in the position of listing mark, and marker and fish body are 45 ° of-60 ° of angles.Sign board ejects and is anchored on fish body by marker grappling is most advanced and sophisticated.After completing, mark to point light pressure symbol location and marker is left to fish body rapidly, sign board is anchored on experiment fish health.After mark, terramycin for Tagging fish (5mg/L) is carried out to soaking disinfection processing.
Described marker is the product of market sale and makes through transformation, comprises main frame, grappling ejection end, sign board holddown groove, striker, trigger five parts compositions (Chien Pey Industry CO., Ltd, Taiwan).By the striker transformation of marker, variation in diameter, consistent with sign board anchored end diameter; Grappling is ejected to end transforms, by its reduced diameter, internal diameter is consistent with sign board anchored end diameter, in the time of listing operation, sign board is installed on to sign board holddown groove, and cocking drives striker to touch sign board anchored end and sign board can be ejected fast by ejecting end.
3. supporting temporarily of Tagging fish
After listing mark completes, the fry of each group is placed in respectively 25m
2cement pit in support temporarily 15d, support temporarily condition: 22 DEG C-24 DEG C of water temperatures, salinity 28-30, pH7.8-8.2, more than dissolved oxygen 5mg/L, inflation, flowing water are cultivated, streamflow is 7-8 flow process.The artifical compound feed of throwing something and feeding during supporting temporarily, feeding volume is the 2-3% of fish body weight, every day clear pond once.During supporting temporarily, record lethality and the de-board rate of each experimental group, the swimming behavior of observation experiment fish and the situation of ingesting.
Support temporarily after 15d at indoor fiberglass tank, Cynoglossus semilaevis mark seed 180 tails of the total length 12cm of surviving are transferred to outdoor ponds (1 mu) and cultivate, in outdoor ponds, jointly cultivate in contrast with the non-marked seed of equal-specification with 180 tails simultaneously.The body length and the body weight that enter pond pre-test experiment fish go out pond after pond culture 60d, are recorded as motility rate and de-board rate, the growing state such as body length, body weight of random measurement 50 tails test fishes.During pond culture, temperature of cultivation 23-26 DEG C, more than dissolved oxygen 7mg/L, cultivating pool changes water approximately 30% every day.During cultivation, the artifical compound feed of throwing something and feeding.
4. result
After listing mark operation, the mark fish Yu Chidi that generally reposes does not move about, and does not ingest.The seed of total length 8cm starts to ingest gradually at 4d, and swimming behavior is normal, and the seed of total length 12cm swimming behavior after marking operation 2d is normal, starts to ingest at 3d, and food ration is passed basically identical with control group gradually in time.The seed of total length 16cm 2d after supporting temporarily recovers normal swimming behavior, and has part seed to start to ingest, and ingests normally at 3d.
With sign board of the present invention, the semi-smooth tongue sole offspring breed of different size is carried out to mark and support temporarily de-board rate and the survival rate after 15d: the seed of average length 16cm, being marked as motility rate is 100%, de-board rate is 0; The seed of average length 12cm: being marked as motility rate is 100%, de-board rate is 0.The seed of average length 8cm: being marked as motility rate is 94%, de-board rate is 4%.
After the outdoor ponds cultivation of 60d, go out pond counting, the survival rate of mark fish reaches 100%, and the growth indexes such as mark fish length, body weight and unmarked seed grow without significant difference (P>0.05), and mark is respond well.
Embodiment bis-:
The 7-11 month in 2010 produces Co., Ltd at Qingdao loyalty seawater and has implemented the correlative study of sign board mark lefteye flounder seed of the present invention, and specific implementation process is as follows:
1, mark fish is selected and experimental scheme
It is normal that mark is with lefteye flounder seed that the loyal seawater in Qingdao produces the color and luster that Co., Ltd cultivates, and size rule is neat, bouncing, and good artificial seed ingests.Seed average body is long is 7cm(body weight 4.9g-7.2g), 9cm(body weight 9.2g-13.4g) and 11cm(body weight 11.5g-15.8g).According to the difference of fry specification, use the sign board (table 1) of 2 kinds of specifications to carry out mark test, 6 mark groups are set altogether, every group of experiment fish 100 tails, every group arranges a repetition.Experiment with seed according to the difference of specification respectively at 4 20m
3cement pit in support temporarily after 3d for token test.Breeding condition during test is supported temporarily with fish: water temperature: 22 DEG C-23 DEG C, salinity: 28-30, pH7.8-8.4, dissolved oxygen content are more than 6mg/L.The manufactured feed of throwing something and feeding during supporting temporarily, feeding volume is 3% of fish body weight, every day, cleaning was cultivated pond once.
The specification of sign board for table 1 test
2, flag operation method
Mark starts the previous day, and mark is stopped eating with fry.Before flag operation starts, all test fishes all carry out dipping bath anesthesia with MS-222, and optimal dose is 80mg/L.In the time listing mark, carry out back listing mark operation with special mark device.Symbol method is with described in embodiment mono-.
3, supporting temporarily of Tagging fish
After the mark of listing completes, the test fish of each group is placed in respectively in the small-size glass steel water tank of diameter 1.2m, high 0.6m, and perennial draingage (day rate of water exchange 800%) is set, and to the disinfection of mark fish, uses 5mg/L terramycin to carry out dipping bath 1 hour.Support temporarily and cultivate 10d, behavior, survival rate and the de-board rate situation of observing during this time mark seed.During supporting temporarily, timing is carried out throwing something and feeding of manufactured feed and is cultivated the cleaning in pond.
Lefteye flounder 180 tails of the average length 9cm taking A sign board mark of surviving are transferred to careat as 25m
2cement pit in lefteye flounder seed (control group) mixed breed of the same specification of the not mark of equivalent amount.The body length and the body weight that enter pond pre-test experiment fish go out pond after industrial aquaculture 60d, record the growing state such as survival rate and body length, body weight, to assess the effect of the external mark of listing.During indoor culture, temperature of cultivation 22-24 DEG C, more than dissolved oxygen 6mg/L, day rate of water exchange approximately 300%, the manufactured feed of throwing something and feeding during this time.
4, result
After listing mark, the experiment fish Yu Chidi that generally reposes does not move about, and does not ingest.The seed of total length 7-9cm starts to ingest after 3-4d after with A and B sign board mark, and swimming behavior is normal, ingests actively.The seed of total length 11cm with two kinds of sign board marks after, can in 2-3d, ingest, swimming behavior is normal.
After A sign board mark, the de-board rate of lefteye flounder seed is 2-5%, and survival rate reaches 94-100%.After B sign board mark, de-board rate be only 4%-6%, and survival rate reaches 93%-97%, shows that the A that develops and B sign board are suitable for the tagging of lefteye flounder seed more than average length 7cm.
After indoor cement pit cultivation 45d, find, the growth indexes such as mark fish length, body weight are without significant difference compared with unmarked control group seed, and survival rate is 100%, and sign board comes off, and mark effect is better.
In actual application, the person skilled of this area completely can be in technical scheme of the present invention other parameter of choose reasonable, but the technical scheme substance of protecting with the present invention is identical, within still falling into protection scope of the present invention.
Claims (3)
1. one kind for the release sign board of external extension of flounder flounder class fingerlings artificial, it is made up of external tag end, connecting line (3) and anchored end (4), external tag end is by indicating that rod (1) and outer tube (2) form, on mark rod, be printed on coded message, outer tube is made up of transparent polypropylene material, and outer tube is closely wrapped in outside mark rod; Flag terminal and anchored end are linked together by connecting line, and connecting line is connected in alignment with one end of flag terminal, and the other end of connecting line is connected with anchored end is vertical; It is characterized in that
Described sign board length (A) 2.8-3.2cm, weight 0.02-0.034g;
Described mark rod length (B) 1.2-1.6cm, weight 0.012-0.021g;
Described connecting line length (C) 1.2-1.6cm;
Described anchored end length (D) 0.6-0.8cm, anchored end diameter (E) 0.5-0.8mm.
2. according to claim 1 a kind of for the release sign board of external extension of flounder flounder class fingerlings artificial, it is characterized in that described mark rod, connecting line and anchored end are all to be made by polyethylene material, safety non-toxic.
3. one kind for the release labeling method of sign board of external extension of flounder flounder class fingerlings artificial, it is characterized in that concrete steps are as follows: be labeled fry and carry the previous day and stop eating, when marking operation, all fries all carry out dipping bath anesthesia with MS-222, marker uses after with 70% alcohol disinfecting, back, fish dorsal fin base portion below is selected to be labeled in the position of listing mark, marker and fish body are 45 ° of-60 ° of angles, after mark completes, extract fast gently marker out to point light pressure symbol location, sign board anchored end is anchored on fry health; After listing mark completes, mark fry continues to put back to indoor culture pond to be cultivated, and mark fish carries out immersion treatment with 5mg/L terramycin, and mark fry is supported time 10-15d temporarily in indoor culture pond.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310013644.XA CN103026998B (en) | 2013-01-15 | 2013-01-15 | Externally-hung marker plate for artificial breeding of flatfish fry and marking method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310013644.XA CN103026998B (en) | 2013-01-15 | 2013-01-15 | Externally-hung marker plate for artificial breeding of flatfish fry and marking method |
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| CN103026998A CN103026998A (en) | 2013-04-10 |
| CN103026998B true CN103026998B (en) | 2014-08-27 |
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| CN201310013644.XA Expired - Fee Related CN103026998B (en) | 2013-01-15 | 2013-01-15 | Externally-hung marker plate for artificial breeding of flatfish fry and marking method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103947589B (en) * | 2014-04-29 | 2016-04-27 | 浙江省海洋水产研究所 | Shrimps flexible label releasing mark method |
| CN105638538B (en) * | 2016-03-30 | 2017-11-24 | 全国水产技术推广总站 | A kind of lefteye flounder ecology mark method for being used to release statistics |
| CN106614216A (en) * | 2016-11-30 | 2017-05-10 | 中国水产科学研究院南海水产研究所 | Method for marking marine shellfishes by means of waterproof labels |
| CN106962254A (en) * | 2017-03-17 | 2017-07-21 | 广东海洋大学 | A kind of sparid class batch denotation approach preferred based on mark effect quantitatively |
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|---|---|---|---|---|
| US4970988A (en) * | 1990-02-01 | 1990-11-20 | Rmc Environmental Services, Inc. | Method and apparatus for monitoring animal migration |
| CN102835339A (en) * | 2012-09-25 | 2012-12-26 | 湖南省水产科学研究所 | In-vitro mark for fish and mark gun thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010119312A (en) * | 2008-11-17 | 2010-06-03 | Chubu Electric Power Co Inc | Method for attaching ic tag |
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2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4970988A (en) * | 1990-02-01 | 1990-11-20 | Rmc Environmental Services, Inc. | Method and apparatus for monitoring animal migration |
| CN102835339A (en) * | 2012-09-25 | 2012-12-26 | 湖南省水产科学研究所 | In-vitro mark for fish and mark gun thereof |
Non-Patent Citations (2)
| Title |
|---|
| 殷禄阁等.牙鲆的增殖放流技术.《河北渔业》.2008,(第7期),第25-27、48页. |
| 牙鲆的增殖放流技术;殷禄阁等;《河北渔业》;20080731(第7期);第25-27、48页 * |
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