CN105532529A - Shellfish pedigree breeding marking contrast method - Google Patents
Shellfish pedigree breeding marking contrast method Download PDFInfo
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- CN105532529A CN105532529A CN201510972978.9A CN201510972978A CN105532529A CN 105532529 A CN105532529 A CN 105532529A CN 201510972978 A CN201510972978 A CN 201510972978A CN 105532529 A CN105532529 A CN 105532529A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to the technical field of aquatic organism selective breeding marking, and relates to a marking contrast method of an offshore intermediate breeding and development stage during a shellfish pedigree breeding process. The invention specifically relates to a shellfish pedigree breeding marking contrast method. According to the method, an environment-friendly plastic pad is adopted as a material for a geometric-shaped marker; different geometric holes are formed in the marker by cutting; and marking can be realized with the holes and the color of the marker. According to the method, the cost is low, and the marking is simple and clear. The marker can be accurately identified under a situation of foreign body adhesion. The recognition degree is high, and the marker meets production needs. The marker is convenient to manufacture, and is easy to carry. The marker can be widely and repeatedly applied. The marker has long preservation time and high preservation rate. Marking is carried out by tying the marker on a cultivation cage with a plastic tie, such that physiological damage to the shellfish is prevented, and the marker is stable and is prevented from falling and loss. Therefore, the stability of the marker is high. A marking rate and readable rate of the marker are both 100%, such that shellfish pedigree breeding large-scale industrialization development requirements are met. The method has an important significance in shellfish industry breeding.
Description
Technical field
The invention belongs to aquatile selection and use labeling technique field, relate to the mark contrast method of marine Transitional culture and forming stage in shellfish family breeding process, be specifically related to a kind of shellfish family breeding mark contrast method.
Background technology
Selection and use is the effective ways of rearing new variety, in animals and plants breeding, obtains extensive success.Wherein, set up family and carry out the important means that systematic breeding is selection and use.At present, existing much about the report of system (family) seed selection both at home and abroad.Excellent family can by screening by generation, the mode of purifying and family's line cross in family, and the additive gene of economic characters in fixed group, increases the gene frequency in colony with economic worth, the final new varieties obtaining people's demand.And the structure of a large amount of family, often need to distinguish, to guarantee that planting matter information between family is not mixed, family labeling technique is arisen at the historic moment.At present, comparatively general family labeling technique mainly comprises cutting appendage, tatoo in shell-fish, deep tissues vital staining, line mark, fluorochrome mark, fluorescent board mark, eye mark, branding etc., cut the types such as fin, chip, closed loop plastic label mark comprising in fish, shellfish industry is as the highest aquaculture industry of China's output, its Breeding Scale constantly expands, in family labeling process, can not adopt in body or body surface marking method one by one individuality mark, and mainly adopt the OEM of external overall aquiculture mesh cage to mark, branding, cut or marker wrote mark, but in breeding process, particularly in marine site mariculture process, notice plate easily comes off loss, and on notice plate be marked at the washing away of seawater under easily fuzzy, difficult identification under fouling organism attachment condition, resolution and readable rate low, directly affect the result of shellfish family breeding.Not yet find at present to be suitable for scale breeding, comparatively convenient and swift and resolution is high, readable strong labeling method.
Summary of the invention
The object of the invention is: in shellfish family breeding process, by designing a kind of simple, convenient, practical shellfish family breeding notice plate, and the relative recording completing notice plate contrast statistical form forms the effective labeling method of system.
For achieving the above object, technical scheme of the present invention is: a kind of shellfish family breeding mark contrast method, and carry out family breeding mark with the notice plate of colored geometry, step is as follows:
(1) notice plate contrast statistical form is set up
Sequence is set up and flag sequence according to breeding family, each family sequence sets up family label and typing notice plate contrasts statistical form respectively, described family sequence and corresponding family establishment record coding, record coding comprises breeding time, breeding batch and family group number information.
Described record coding is: the shellfish breeding family selecting eight Arabic numerals corresponding method record different year different batches, and former four Arabic numerals represent the breeding time; Breeding batch is represented with the 5th Arabic numerals; Using last three Arabic numerals as family group number marker code, with family sequence pair answer mark shown in the hole of different geometries on notice plate corresponding.
(2) notice plate is set up
Corresponding each family sequence, set up the notice plate of a geometry, contrast statistical form with notice plate and carry out corresponding record, described notice plate is colored, it is batch corresponding that the color of notice plate and mark contrast breeding in record sheet, and namely the family sequence of same batch marks with same color notice plate; On notice plate, the hole of diverse location cutting different geometries is in a certain direction as marker code, the hole of diverse location, different geometries represents different quantity ranks, and it is corresponding that the quantity representated by marker code and notice plate contrast the family group number that the breeding family sequential recording in statistical form encodes.
The described geometry setting up the notice plate of a geometry does not comprise the holohedral symmetry geometric figures such as circular.
Described notice plate selects environment-friendly type plastic backing plate to be material.
The hole of described diverse location cutting different geometries in a certain direction on notice plate is as marker code, its geometry can adopt circle and semicircle, or vee, circular and semicircular indentations office card punch completes, the vee scissors cutting at notice plate edge.
(3) notice plate tying-up is in the enterprising row labels of aquiculture mesh cage
In the seed outbound stage, same family seed uses same notice plate, notice plate makes multiple according to family sequence seed quantity and input adherance quantity, and every sheet adherance makes a notice plate, and seed outbound stage and adherance are together put into nursery mesh bag and marked.
After seed loads cylinder mould, be tying-up instrument, penetrated by band line by notice plate inner circular aperture with Plastic Bandage, be connected with mark family cylinder mould lifting rope, after connecting, band penetrates buckle, completes mark.
The present invention, in shellfish family breeding process, is marked shellfish breeding family by the notice plate setting up notice plate contrast statistical form and the simple readability of making.The different family sequence of the method clear resolution of energy, make shellfish breeding mark convenient and swift and resolution is high, readable strong, facilitate the estimation of later stage family breeding value and correlated inheritance parameter, after breeding marks 180 days, add up family notice plate, mark rate and the readable rate of its family are 100%, and family notice plate is to shellfish growth, no effect on survival, directional cross when being beneficial to breeding, not only can prevent inbreeding depression but also can improve the Main Economic Characters of offspring fast.
The invention has the beneficial effects as follows: adopt environmentally-friendly plastic backing plate as the material of geometry notice plate, on notice plate, cutting different geometries hole and notice plate color are as mark mode, with low cost, mark simple and clear, under foreign matter adhesion condition, also can accurate discrimination, resolution is high, meets Production requirement; And notice plate is easy to make, portable, can extensively, reuse, mark the holding time on, storage rate is high; Notice plate tying-up in the enterprising row labels of aquiculture mesh cage, can not be caused shellfish physiological damage by use Plastic Bandage, and mark stablizes difficult drop-off, loss, stable high.Mark rate of the present invention and readable rate are 100%, meet scale shellfish family breeding industries demand for development, significant to the stock breeding of shellfish industry.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention 1 notice plate;
In figure: 1. each circular hole represents 1,2. each half bore represents 5, and 3. each half bore represents 10; 4. each triangle represents 50, and 5. each triangle represents 100.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
For applicant's Patinopecten yessoensis family in spring breeding in 2013 experiment, introduce shellfish family breeding mark contrast method, step is as follows:
(1) notice plate contrast statistical form is set up
Patinopecten yessoensis family in 2013 breeding test in January 11 kind shellfish binning phase formally, lay eggs February 22 through indoor conditionting culture, ejaculation, insemination, then family is set up, flag sequence, family breeding in 2013 experiment has amounted to the family breeding experiment of batch, set up family 200 groups and 200 groups of familys are marked, namely corresponding 200 groups of family sequences are set up record coding and are comprised the breeding time, breeding batch marks with breeding quantity, family sequential recording is encoded to: the shellfish breeding family selecting eight Arabic numerals corresponding method record different year different batches, four Arabic numerals represented the breeding time in the past, in the present embodiment, the time is 2013, represent breeding batch with the 5th Arabic numerals, in the present embodiment only one batch, be 1, using last three Arabic numerals as reference numerals code, be 001 to 200 in this experiment, with family sequence pair answer mark shown in the hole of different geometries on notice plate corresponding.
Corresponding each family sequence sets up family label, namely sets up family label 200.The record coding that mark family sequence is set up and family label typing notice plate contrast statistical form respectively, make it family label corresponding with experiment sequence number, refer to table 1, and collect breeding scallop Parent according to different family label, record breeding family mating strategy and Parent dominant phenotype characteristic index: the information such as shell is high, shell is long, shell is wide, weight in wet base.Record family Parent information 400 in this experiment, separately count in Parent information table.
(2) notice plate is set up
Breeding family is after hatching, seed selection, larva cultivation, juvenile mollusk are cultivated, and then marine Transitional culture is carried out in outbound.Before the outbound of Patinopecten yessoensis experiment family, corresponding each family sequence sets up the notice plate of a geometry, contrast statistical form with notice plate and carry out corresponding record, notice plate adopts environment-friendly type plastic backing plate to be raw material, this time test only one batch, family 200 groups, therefore notice plate selects same color, the different colours of notice plate represents different batches, selects 200 notice plates making same color-blueness.For clear mark, in the seed outbound stage, same family seed uses same notice plate, and notice plate makes multiple according to family sequence seed quantity and input adherance quantity, every sheet adherance makes a notice plate, goes out seed storehouse stage and adherance and together puts into nursery mesh bag and mark.On notice plate, the hole of diverse location cutting different geometries is in a certain direction as marker code, the hole of diverse location, different geometries represents different quantity ranks, it is corresponding that quantity representated by marker code and notice plate contrast the family group number that the breeding family sequential recording in statistical form encodes, in the present embodiment, this stage at least makes 200 notice plates, correspondingly on family notice plate statistical form, records seed outbound mesh bag quantity.
The length of the plastic base plate raw material in the present embodiment is 29.8cm, and wide is 19.8cm, is cut out by plastic base plate grow into 4.95cm, the wide rectangular piece for 3.725cm, i.e. notice plate according to 4 row 8 row modes; As shown in Figure 1, this notice plate adopts circular port and semicircle, vee carry out punching mark, the half round cut office card punch at circular port and notice plate edge completes, the vee scissors cutting at notice plate edge; The notice plate of corresponding 1-5 group family, beat 1-5 circular hole respectively along notice plate left upper portion and mark, the order of magnitude that each circular hole represents is 1, to see in Fig. 1 1.; The notice plate of the family of corresponding 6-10 group, first half round cut is beaten at notice plate left side edge, the order of magnitude of this position half round cut representative is 5, to see in Fig. 1 2., then beat circular hole along notice plate left upper portion, the quantity sum making the quantity of circular hole representative on notice plate face add the representative of left side semicircle breach is 6-10; When in family sequence, family group number marker code is greater than 10, mark along beating half round cut with notice plate upper edge, the order of magnitude that this position half round cut represents is 10, to see in Fig. 1 3.; When in family sequence, family group number marker code is greater than 50, on the right side of notice plate, cutting vee marks, and the order of magnitude of this position triangle breach representative is 50, to see in Fig. 1 4.; When in family sequence, family group number marker code is greater than 100, mark at notice plate lower edges cutting vee, the order of magnitude of this position triangle breach representative is 100, to see in Fig. 1 5.; Can obtaining Fig. 1 notice plate according to this, to represent family sequence be 20131200, and make a concrete analysis of as on notice plate, 5 circular ports represent 5, left side edge 1 half round cut represents 5,5+5=10; Notice plate upper edge is along there being 4 half round cut to represent 40; Right edge represents 50 along 1 vee, and lower edges 1 vee represents 100, and therefore, 10+40+50+100=200, it is 20131200 that this notice plate represents family sequence code.
The record coding of the mark contrast record sheet that Fig. 1 family sequence pair is answered is 20131200, represent first breeding in 2013 No. 200 family sequence, namely family query note is encoded for this reason, and its last 3 reference numerals codes mark family sequence set number with corresponding notice plate and mark identical; Conveniently check inquiry.
(3) notice plate tying-up is in the enterprising row labels of aquiculture mesh cage
After seed loads cylinder mould, be tying-up instrument, penetrated by band line by notice plate inner circular aperture with Plastic Bandage, be connected with mark family cylinder mould lifting rope, after connecting, band penetrates buckle, completes mark; Aquiculture mesh cage marks through notice plate, uses band tying-up, can accomplish not come off under natural environmental condition, nondiscolouring, can identification, can use for a long time in shellfish family seed rearing process.
Within 2013, applicant carries out Patinopecten yessoensis extensive family breeding experiment in spring, and seed, through fertilization, hatching, is developed to D type larva; Later stage cultivates through floating larvae, and the metamorphosis of shell top occurs eyespot to about 200um, starts to throw in adherance; Eyebot larvae, through settlement and metamorphosis, is grown to juvenile mollusk gradually; Juvenile mollusk at indoor growing to about 600um, be moved to 60 order mesh bags indoor and in the greater coasting area of off-lying sea Transitional culture.For mark different experiments family, prevent from mixing between family, before seed outbound, carry out experiment notice plate successively, notice plate is put into outbound mesh bag, and be labeled seed and be jointly transferred to mariculture, now, carry out notice plate contrast statistical form relative recording simultaneously; Mid-June, seed is backhauled and carries out a point seedling, is cleaned out by notice plate, jointly carries out changing bag with mark family seed; In July, when seed grows to about 1cm, family seed is entered cage, after entering cage, notice plate uses Plastic Bandage to carry out tying-up, labelling experiment family, and the later stage falls the operations such as cage, the work such as carry out the recovery of notice plate, increase, cleaning, supplement.Notice plate is up to 4 years service life, and mark rate 100% can produce breakage under natural environment, but because making easily, can now do existing benefit, and with low cost, and average unit cost, less than 0.1 yuan, therefore can extensively be promoted the use of.
Table 1. notice plate contrast statistical form
Claims (5)
1. a shellfish family breeding mark contrast method, it is characterized in that, carry out family breeding mark with the notice plate of colored geometry, step is as follows:
(1) notice plate contrast statistical form is set up
Sequence is set up and flag sequence according to breeding family, each family sequence sets up family label and typing notice plate contrasts statistical form respectively, described family sequence and corresponding family establishment record coding, record coding comprises breeding time, breeding batch and family group number information;
(2) notice plate is set up
Corresponding each family sequence, set up the notice plate of a geometry, contrast statistical form with notice plate and carry out corresponding record, described notice plate is colored, it is batch corresponding that the color of notice plate and mark contrast breeding in record sheet, and namely the family sequence of same batch marks with same color notice plate; On notice plate, the hole of diverse location cutting different geometries is in a certain direction as marker code, the hole of diverse location, different geometries represents different quantity ranks, and it is corresponding that the quantity representated by marker code and notice plate contrast the family group number that the breeding family sequential recording in statistical form encodes;
(3) notice plate tying-up is in the enterprising row labels of aquiculture mesh cage
In the seed outbound stage, same family seed uses same notice plate, notice plate makes multiple according to family sequence seed quantity and input adherance quantity, and every sheet adherance makes a notice plate, and seed outbound stage and adherance are together put into nursery mesh bag and marked;
After seed loads cylinder mould, be tying-up instrument, penetrated by band line by notice plate inner circular aperture with Plastic Bandage, be connected with mark family cylinder mould lifting rope, after connecting, band penetrates buckle, completes mark.
2. a kind of shellfish family breeding mark contrast method according to claim 1, it is characterized in that, described record coding is: the shellfish breeding family selecting eight Arabic numerals corresponding method record different year different batches, and former four Arabic numerals represent the breeding time; Breeding batch is represented with the 5th Arabic numerals; Using last three Arabic numerals as family group number marker code, with family sequence pair answer mark shown in the hole of different geometries on notice plate corresponding.
3. a kind of shellfish family breeding mark contrast method according to claim 1, it is characterized in that, the described geometry setting up the notice plate of a geometry, does not comprise holohedral symmetry geometric figure.
4. a kind of shellfish family breeding mark contrast method according to claim 1, it is characterized in that, described notice plate selects environment-friendly type plastic backing plate to be material.
5. a kind of shellfish family breeding mark contrast method according to claim 1, it is characterized in that, the hole of described diverse location cutting different geometries in a certain direction on notice plate is as marker code, its geometry can adopt circle and semicircle, or vee, circular and semicircular indentations office card punch completes, the vee scissors cutting at notice plate edge.
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| CN201510972978.9A CN105532529B (en) | 2015-12-23 | 2015-12-23 | A kind of shellfish family breeding marks contrast method |
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Cited By (4)
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| CN108450378A (en) * | 2018-01-24 | 2018-08-28 | 广西壮族自治区海洋研究所 | A kind of construction method of Shi Shi Le selection and breeding family |
| CN113287553A (en) * | 2021-06-29 | 2021-08-24 | 广西海洋研究所有限责任公司 | Marettes margaritifera marking and measuring breeding cage |
| CN114973912A (en) * | 2022-05-16 | 2022-08-30 | 海南大学 | Label for experiment and experiment labeling method |
| CN115053840A (en) * | 2022-05-24 | 2022-09-16 | 广西海洋研究所有限责任公司 | Construction method of buried shellfish dynamic germplasm resource library |
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