CN108064773A - A kind of method that saline-alkali water raises and train colored perch juvenile fish - Google Patents
A kind of method that saline-alkali water raises and train colored perch juvenile fish Download PDFInfo
- Publication number
- CN108064773A CN108064773A CN201711391885.2A CN201711391885A CN108064773A CN 108064773 A CN108064773 A CN 108064773A CN 201711391885 A CN201711391885 A CN 201711391885A CN 108064773 A CN108064773 A CN 108064773A
- Authority
- CN
- China
- Prior art keywords
- salinity
- perch
- water
- flower
- saline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000269799 Perca fluviatilis Species 0.000 title claims abstract description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000000366 juvenile effect Effects 0.000 title claims abstract description 13
- 239000003513 alkali Substances 0.000 title claims abstract description 8
- 239000013535 sea water Substances 0.000 claims abstract description 19
- 239000008399 tap water Substances 0.000 claims abstract description 13
- 235000020679 tap water Nutrition 0.000 claims abstract description 13
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000009395 breeding Methods 0.000 claims abstract description 9
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 229910001425 magnesium ion Inorganic materials 0.000 claims abstract description 9
- 238000012545 processing Methods 0.000 abstract description 25
- 235000013601 eggs Nutrition 0.000 abstract description 23
- 230000002159 abnormal effect Effects 0.000 abstract description 16
- 230000012447 hatching Effects 0.000 abstract description 15
- 230000004083 survival effect Effects 0.000 abstract description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 7
- 239000011780 sodium chloride Substances 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000002689 soil Substances 0.000 abstract 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 14
- 239000011575 calcium Substances 0.000 description 14
- 150000002500 ions Chemical class 0.000 description 11
- 229910052791 calcium Inorganic materials 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 239000013589 supplement Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 229910001414 potassium ion Inorganic materials 0.000 description 8
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 7
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 description 7
- 229910001424 calcium ion Inorganic materials 0.000 description 7
- 239000011777 magnesium Substances 0.000 description 7
- 229910052749 magnesium Inorganic materials 0.000 description 7
- 230000001681 protective effect Effects 0.000 description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241001417495 Serranidae Species 0.000 description 3
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229910001415 sodium ion Inorganic materials 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 241000252234 Hypophthalmichthys nobilis Species 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 241000276618 Perciformes Species 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 230000036732 histological change Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- SWHAQEYMVUEVNF-UHFFFAOYSA-N magnesium potassium Chemical compound [Mg].[K] SWHAQEYMVUEVNF-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 238000007492 two-way ANOVA Methods 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000321429 Epinephelus itajara Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001275923 Gymnocypris Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000755266 Kathetostoma giganteum Species 0.000 description 1
- 241000269794 Lateolabrax Species 0.000 description 1
- 241001287943 Lateolabrax maculatus Species 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 208000000875 Spinal Curvatures Diseases 0.000 description 1
- 241000722085 Synanceia horrida Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000003926 complexometric titration Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000000705 flame atomic absorption spectrometry Methods 0.000 description 1
- 210000001647 gastrula Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000019600 saltiness Nutrition 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of methods that saline-alkali water raises and train colored perch juvenile fish.In flower perch incubating oosperm, it is 20~30 to set salinity；In flower perch newly hatched larvae period, it is 25~30 to set salinity, and the adjusting of salinity is prepared in proportion by using seawater with tap water, and keeps Ca in water2+Concentration is more than 194.26mg/L, Mg2+Concentration is more than 556.67mg/L, and pH is 6.5~7.5, breeding water body basicity≤20mmol/L.The beneficial effects of the invention are as follows propose a kind of as the saline and alkaline water body in the north to introduce a fine variety the application of colored perch and cultural technique method, the northern marine site flower perch breeding suitable salinity of tool and the setting of pH processing, flower perch fertilized eggs and the newly hatched larvae hatching of the present invention and cultural method, which have, spends perch hatchability of fertile eggs high, abnormal rate is low and prelarva survival activity is strong, and theoretical foundation is provided for the salt-soda soil cultivation of early stage flower perch seed.
Description
Technical field
The invention belongs to technical field of aquaculture, are related to a kind of method that saline-alkali water raises and train colored perch juvenile fish.
Background technology
Flower perch (Lateolabraxmaculatus) is subordinate to Perciformes (Perciformes), Sushi sections (Serranidae), flower
Perch category (Lateolabrax) is commonly called as perch, jewfish, and tool growth is fast, disease is few, adapts to the features such as wide.It is supported by initial stage port, pond
Cultivation, develops to the high density intensive culture mode such as modern seawater cage, batch production.In recent years northern China is coastal has broken through flower
The full artificial breeding technology difficulty of perch, this will increase northern area flower perch seed supply, and then drive north Coast and internal water
Domain perch aquaculture flourishes.Flower perch early stage likes river mouth salt-fresh water waters of dwelling, in the Hekou Area of salt-fresh water, Low-salinity
The features such as tool growth is fast, and meat is good.Chinese inland waters degree of saltiness distribution of water resources is irregular, the euryhalinous biological characteristics of flower perch
Property be just adapted with it, but fish early development stage is more sensitive to extraneous environmental change, easily causes irreversible influence, therefore
Carrying out assessment to the growth performance of flower perch early stage is particularly important.
The typical water body of different saline and alkalines relatively embodies a concentrated reflection of in the province of northwest：Such as the Qinghai Lake in Qinghai Province, Xinjiang
Ebinur Lake, Ulungur Lake, Bosten, Chai Wo-pu Lake, Dai Hai, Hulun nur, Da Lihu, the Ulansuhai Nur in the Inner Mongol.More than area
Be water body mostly based on brackish water, breed variety is single, and slow-growing, mainly cultivates crucian, graining, Gymnocypris prezewalskii etc.
Traditional fish.And perch is spent as wide temperature, eurysaline fishes, it can be as the high added value fish that these waters are raised and train.This patent with
Yellow River System is served as theme, and China's saline and alkaline water body of typical case is counted.Statistical result showed, each typical case's water body can divide three classes：It is small
In the weak base type water body (pond, reservoir, lake) of 5mmol/L；5~18mmol/L moderate alkaline water body (estuary, lake)；Greatly
In the high-alkali lake type water bodys of 18mmol/L.Statistics is carried out to each water salinity to know, though each water body basicity, ion composition and total amount are deposited
In larger difference, but its overall salinity is not less than 15 ‰.It is now recognized that 10mmol/L can be used as silver carp
The basicity Danger Indexes of (Hypophthalmichehys molitrix), flathead (Aristichthys nobilis) breeding water,
And more by force the basicity Danger Indexes of perch is spent to have not been reported resistance.
The content of the invention
It is an object of the invention to provide a kind of methods that saline-alkali water raises and train colored perch juvenile fish.
The technical solution adopted in the present invention is in flower perch incubating oosperm, and it is 20~30 to set salinity；Flower perch just incubates
In prelarva period, it is 25~30 to set salinity, and the adjusting of salinity is prepared in proportion by using seawater with tap water, and is kept in water
Ca2+Concentration is more than 194.26mg/L, Mg2+Concentration is more than 556.67mg/L, and pH is 6.5~7.5, and breeding water body basicity≤
20mmol/L。
Further, flower perch transplanting specification is average weight per more than tail 0.8g.
The beneficial effects of the invention are as follows propose a kind of application and cultural technique side that colored perch is introduced a fine variety as the saline and alkaline water body in the north
Method has the setting of northern marine site flower perch breeding suitable salinity and pH processing, flower perch fertilized eggs of the invention and newly hatched larvae hatching
Have flower perch hatchability of fertile eggs high with cultural method, abnormal rate is low strong with prelarva survival activity, saline and alkaline for early stage flower perch seed
Ground cultivation provides theoretical foundation.
Description of the drawings
Fig. 1 is hatching rate and abnormal rate figure of the colored perch fertilized eggs under the conditions of different salinity (a) and pH value (b)；
Fig. 2 is that different salinity handles initial weight 0.10607g/ tails specification flower perch juvenile fish changes of body mass；
Fig. 3 is that different salinity handles initial weight 0.84341g/ tails specification flower perch juvenile fish changes of body mass；
Fig. 4 is that different salinity handles initial weight 10.94173g/ tails specification flower perch juvenile fish changes of body mass；
Fig. 5 be I age flower perch juvenile fish through different salinity (0 ‰, 12 ‰, 30 ‰, 45 ‰) cultivate 30d after histological change.
Specific embodiment
The present invention is described in detail With reference to embodiment.
Embodiment 1
1 experiment material
1.1 fertilized eggs sources
Experiment is purchased from Guangdong Jiangmen aquatic farm with fertilized eggs, is that the parent population of ALONG COASTAL FUJIAN cage culture is temporary through interior
It supports, obtained after artificial induced spawning and insemination.Under the conditions of 16 DEG C of water temperature, fertilization ovum collecting finishes and is transported to Zhuhai City Doumen District river mouth
Primitive gut late period is in during fisheries research laboratory.Normotrophic fertilized eggs are selected to test for salinity.
1.2 experimental water sources
Experiment is Zhuhai coastal seawater with seawater, and Zhuhai City Doumen District estuarine fishery research institute is transported to by waterr transporting vehicle.It is light
Water is the tap water of Zhuhai waterworks supply.CaCl is used respectively2、MgCl2, KCl (analysis pure) added in tap water potassium from
Son, calcium and magnesium ion, calcium, magnesium, potassium ion to seawater concentration with obtain addition potassium tap water, add calcium and magnesium tap water and
Add the tap water of calcium and magnesium potassium.Salinity is measured with ATAGO hand-hold refractometers, with EDTA complexometric titration calcium and magnesium ions
Concentration, with the sodium of Flame Atomic Absorption Spectrometry Determination, potassium concentration, each experimental water ion concentration such as (table 1).More than it is real
It tests and is all tested with water after fully precipitation and aeration for salinity.Table 1 is colored perch newly hatched larvae in not bait throwing in, different salinity condition
Under survival rate and survival activity index, note：The numerical value that different letters are indicated in same row represents significant difference (P ＜ 0.05),
Similarly hereinafter.
Table 1
2 experimental methods
The sink-float experiment of fertilized eggs under the conditions of 2.1 different salinities
12 salinity gradients are set, and salinity is respectively：20、21、22、23、23.4、24.3、25.2、26、27、28、29、
30.It is configured and obtained by a certain percentage with seawater and tap water.With 500mL graduated cylinders carry out fertilized eggs sink-float property experiment, take every time by
Smart 200, ovum stablizes 10min after putting into graduated cylinder from fertilized eggs, observes and records presence of the fertilized eggs in different salinity seawater
State.
Incubating oosperm is tested under 2.2 different salinities and ionic conditions
Totally 5 salinity gradients, each salinity set A (normal), B (supplement potassium ions to seawater for setting 25,20,15,10,5
Concentration), C (supplement calcium and magnesium ion to concentration of seawater), D (supplement calcium, magnesium, potassium ion to concentration of seawater) totally 4 different ions
Processing is (wherein because B, D group Na of salinity 10 and 5+：K+Than relatively low, so being not provided with), seawater control group salinity is 30.Every group
Setting 3 is parallel, totally 51 polyethylene hatching cups.Wherein A processing is configured by a certain percentage with seawater with tap water；B processing is used
Seawater configures by a certain percentage with adding the tap water of potassium；C processing seawater is matched somebody with somebody by a certain percentage with adding the tap water of calcium and magnesium
It puts；D processing is configured by a certain percentage with seawater with adding the tap water of calcium and magnesium potassium.Each processing group uses ATAGO hand-held refractive powers
Instrument demarcates salinity, ion concentration such as (table 2).
Table 2 is survival rate and survival activity index of the colored perch newly hatched larvae under not bait throwing in, condition of different pH；
Each processing group take 200 fertilized eggs be put into 2000mL polyethylene hatching cup in hatch, it is micro- inflation to avoid Low-salinity
Fertilized eggs in group sink to the bottom accumulation death, and all processing groups are placed in same 160cm × 70cm × 20cm square sinks, with heating
Stick control 16.5~17.5 DEG C of water temperature of hatching, pH7.9~8.0, indoor incandescent lamp keep intensity of illumination for 400~500LUX (on
Extra large Jiading student's federation industry JD-3 types illumination photometer measures).After prelarva hatches, hatching rate and newly hatched larvae abnormal rate (deformity son are recorded
Fish refers to afterbody bending, the individual of spinal curvature).
Since the content of sodium ion in seawater is far above calcium, magnesium, potassium ion, do not discuss in the identical salinity of this experiment add from
Son and caused by sodium ion total amount difference, only discuss therefore caused by Na+：K+The variation of ratio.2.3 different salinities and ionic conditions
The survival activity experiment of lower newly hatched larvae
It is tested from upper one section in the prelarva that each processing group hatches, randomly selects 50 tail health newly hatched larvaes and be put into corresponding salt
The 2000mL plastic cups of degree and ionic conditions (wherein remove extremely low 10A, 10C, 5A, 5C group of healthy prelarva number), and no bait throwing in is quiet
Water planting is educated, daily to draw dead prelarva 2 times (tactile unresponsive for death with glass bar parent), records dead prelarva number, starvation is extremely
Experiment terminates when prelarva is all dead.Calculate newly hatched larvae survival activity (survivalactivityindex, SAI).
2.4 index determining
Testing index in this research mainly includes hatching rate, abnormal rate, SAI values.
Hatching rate=total ovum number × 100% of rupture of membranes prelarva number/hatching；
Abnormal rate=deformity prelarva number/rupture of membranes prelarva number × 100%；
2.5 data analysis
ANOVA one-way analysis of variances are carried out to hatching rate, abnormal rate, SAI values with SPSS statistical softwares (19.0), and
DUNCAN Multiple range tests are carried out, are to have significant difference with P ＜ 0.05；Two-way analysis of variance is carried out to SAI values.All data
(Mean ± SD) is represented with average ± standard deviation, is finally mapped with EXCEL2013.
3 results
The sink-float of flower perch fertilized eggs under the conditions of 3.1 different salinities
Distribution of the fertilized eggs in different salinity seawater is different.When salinity is less than 23, fertilized eggs all sink to bottom
Portion；When salinity is more than 27, water surface is all floated on；For salinity at 23.4~26, fertilized eggs have part to suspend in water, wherein
It is whole suspensions when salinity is 25, as shown in Figure 1, different upper case or lower case letters are noted in Fig. 1 represents significant difference (P<
0.05)。
The hatching rate and abnormal rate of flower perch fertilized eggs under the conditions of 3.2 different salinities and ion composition
Fig. 2 shows the influence of salinity and ion composition to hatchability of fertile eggs and abnormal rate.Same number of days, different salinity
Between, different letters represent significant difference (P<0.05).Between same salinity, different number of days, * represents significant difference (P<
0.05).The average percentage hatch rate of 15~30 processing group of salinity is both greater than 90%；10 processing group average percentage hatch rate of salinity is more than 80%,
5 processing group average percentage hatch rate of salinity is 5.3%, but interior whole is dead for 24 hours after rupture of membranes for the newly hatched larvae of 5 and 10 processing group of salinity
It dies；In same salinity, ion composition does not have a significant impact to hatching rate (P ＞ 0.05).In the processing group of salinity 20~30,
Abnormal rate is below 5%；The processing group of salinity 5, abnormal rate average out to 97.5%；The abnormal rate of 10~15 processing group of salinity is
8.4%~33.4%, in same salinity, ion composition has a significant impact to abnormal rate (P ＜ 0.05), wherein B (supplement potassium
Ion group) abnormal rate be significantly higher than A (normal group) and D (supplement calcium, magnesium, potassium ion group), C's (supplement calcium and magnesium ion group)
Abnormal rate is substantially less than A and D processing (P ＜ 0.05).
The SAI values of flower perch newly hatched larvae under 3.3 different salinities and ionic conditions
Salinity, ion composition and have pole conspicuousness shadow at salinity from molecular reciprocation to the SAI values of newly hatched larvae
It rings (P ＜ 0.01) (Fig. 3 and table 4).In Fig. 3 between same salinity, different number of days, different letters represent significant difference (P<
0.05).Between same number of days, different salinity, * represents significant difference (P<0.05).30 and 25 processing group SAI values of salinity are maximum,
In 15~25 processing group of salinity, SAI values are reduced with the reduction of salinity.The processing group intermediate ion of salinity 25 is formed to SAI values
Influence not significantly (P ＞ 0.05)；In 15 and 20 processing group of salinity, ion forms (the P ＜ that have a significant impact to SAI values
0.05).Wherein in the processing group of salinity 20, the SAI values of A (normal group) and C (supplement calcium and magnesium ion group) are higher than B (supplement potassium
Ion group) and D (supplement calcium, magnesium, potassium ion group)；In the processing group of salinity 15, the SAI value highests of C groups, the SAI values of B and D groups
Minimum (table 3).
The influence of 4 two-way analysis of variance salinity of table and ion composition to flower perch newly hatched larvae SAI values
Fig. 4 is that different salinity handles initial weight 10.94173g/ tails specification flower perch juvenile fish changes of body mass；
Note：Between same salinity, different number of days, different letters represent significant difference (P<0.05).Same number of days, different salt
Between degree, * represents significant difference (P<0.05).Fig. 5 is that (0 ‰, 12 ‰, 30 ‰, 45 is ‰) foster through different salinity for I age flower perch juvenile fish
Grow the histological change after 30d.
Present invention discover that when salinity is less than 23, flower perch fertilized eggs all sink to water layer bottom；When salinity is more than 27, all
Float on water layer surface；For salinity at 23.4~26, fertilized eggs have part to suspend in water.Flower perch fertilized eggs suitable for hatching salinity be
15~30, optimal salinity of hatching is 20~30；The suitable salinity scope of flower perch newly hatched larvae is 20~30, and optimal salinity scope is
25~30.Work as Ca2+≤194.26mg/L、Mg2+The abnormal rate of colored perch incubating oosperm can be increased during≤556.67mg/L, reduced
The vigor of prelarva.
Advantage also resides in technical solution of the present invention compared with prior art：
1st, a large amount of freezen protectives of seven band stonefish sperms and application are realized, it can be in breeding early period or later stage using this method
Mature sperm freezen protective is got up spare or from domestic other local sperms of the freezen protective seven with grouper, transported
Nursery.Solve the problems, such as that semen volume is insufficient in seven band grouper artificial propagations.
2nd, spermatozoa diluent ingredient is simple, and freezen protective is at low cost, and dilution only has 3 kinds of compositions：Glucose 60g/L,
NaCl 10g/L、NaHCO3 0.5g/L。
3rd, freezen protective amount is big, sperm motility is high, and the 0.25ml straws of refrigerating capacity from the prior art increase to 2.0ml jellies
Deposit pipe, the frozen sperm vigor that is averaged is increased to 72.92% from 10%.It is suitable for establishing sperm freezing storehouse, in fish production, heredity
It is promoted and applied in breeding and preserving seed.
4th, freezing and storing method is simple, easy to spread, can directly carry out freezen protective operation at the scene, reach practicality
Change horizontal, the fertility rate and hatchability of frozen sperm respectively reaches 68.08%, 76.83%, and the rate of fertilization of fresh spermatozoa and incubates
Rate is respectively 69.87 ± 6.05%, 64.33 ± 4.04%, and there was no significant difference (P ＞ 0.05) with fresh essence for frozen sperm.
The above is only the better embodiment to the present invention, not makees limit in any form to the present invention
System, any simple modification that every technical spirit according to the invention makes embodiment of above, equivalent variations and modification,
Belong in the range of technical solution of the present invention.
Claims (2)
1. a kind of method that saline-alkali water raises and train colored perch juvenile fish, it is characterised in that：In flower perch incubating oosperm, setting salinity is
20~30；In flower perch newly hatched larvae period, it is 25~30 to set salinity, and the adjusting of salinity is matched somebody with somebody in proportion by using seawater with tap water
System, and keep Ca in water2+Concentration is more than 194.26mg/L, Mg2+Concentration is more than 556.67mg/L, and pH is
6.5~7.5, breeding water body basicity≤20mmol/L.
2. the method for colored perch juvenile fish is raised and train according to a kind of saline-alkali water described in claim 1, it is characterised in that：The colored perch transplanting
Specification is average weight per more than tail 0.8g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711391885.2A CN108064773A (en) | 2017-12-21 | 2017-12-21 | A kind of method that saline-alkali water raises and train colored perch juvenile fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711391885.2A CN108064773A (en) | 2017-12-21 | 2017-12-21 | A kind of method that saline-alkali water raises and train colored perch juvenile fish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108064773A true CN108064773A (en) | 2018-05-25 |
Family
ID=62158712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711391885.2A Pending CN108064773A (en) | 2017-12-21 | 2017-12-21 | A kind of method that saline-alkali water raises and train colored perch juvenile fish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108064773A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122380A (en) * | 2019-06-04 | 2019-08-16 | 利津县双瀛水产苗种有限责任公司 | A kind of north waters calico bass artificial breeding method |
| CN114680064A (en) * | 2022-04-24 | 2022-07-01 | 中国水产科学研究院黑龙江水产研究所 | Adaptive culture method for fish in northeast saline-alkali pond |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3773802A (en) * | 1969-09-05 | 1973-11-20 | Basf Ag | N-arylsulfonyl-n'-2-tetrahydrodicyclopentadienylureas |
| CN1404708A (en) * | 2001-12-29 | 2003-03-26 | 中国科学院长春地理研究所 | Method for utilizing carbonate type bogged heavy saliue-alkali lake for fishery industry |
| CN1465231A (en) * | 2002-10-12 | 2004-01-07 | 中国科学院长春地理研究所 | High yields fish culturing method for carbonate type heavy salinized small reservoir |
| CN101675729A (en) * | 2008-09-16 | 2010-03-24 | 中国水产科学研究院东海水产研究所 | Method for cultivating and artificial breeding plateau saline-alkali water area northern pike |
| CN105475184A (en) * | 2015-11-18 | 2016-04-13 | 中国海洋大学 | Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method |
-
2017
- 2017-12-21 CN CN201711391885.2A patent/CN108064773A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3773802A (en) * | 1969-09-05 | 1973-11-20 | Basf Ag | N-arylsulfonyl-n'-2-tetrahydrodicyclopentadienylureas |
| CN1404708A (en) * | 2001-12-29 | 2003-03-26 | 中国科学院长春地理研究所 | Method for utilizing carbonate type bogged heavy saliue-alkali lake for fishery industry |
| CN1465231A (en) * | 2002-10-12 | 2004-01-07 | 中国科学院长春地理研究所 | High yields fish culturing method for carbonate type heavy salinized small reservoir |
| CN101675729A (en) * | 2008-09-16 | 2010-03-24 | 中国水产科学研究院东海水产研究所 | Method for cultivating and artificial breeding plateau saline-alkali water area northern pike |
| CN105475184A (en) * | 2015-11-18 | 2016-04-13 | 中国海洋大学 | Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method |
Non-Patent Citations (2)
| Title |
|---|
| 杨明秋等: "温度、盐度和pH值对豹纹鳃棘鲈早期发育的影响", 《热带生物学报》 * |
| 樊国平: "盐碱水域养殖鲈鱼技术初探", 《内陆水产》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122380A (en) * | 2019-06-04 | 2019-08-16 | 利津县双瀛水产苗种有限责任公司 | A kind of north waters calico bass artificial breeding method |
| CN114680064A (en) * | 2022-04-24 | 2022-07-01 | 中国水产科学研究院黑龙江水产研究所 | Adaptive culture method for fish in northeast saline-alkali pond |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Poltermann et al. | Life under Arctic sea ice–reproduction strategies of two sympagic (ice-associated) amphipod species, Gammarus wilkitzkii and Apherusa glacialis | |
| CN105379651A (en) | Method for salinity domestication of lateolabraxjaponicus fry | |
| Crosbie et al. | Impact of thermoclines on the vertical distribution of salmon lice larvae | |
| Villanueva et al. | Embryonic life of the loliginid squid Loligo vulgaris: comparison between statoliths of Atlantic and Mediterranean populations | |
| Bir et al. | The effects of different water quality parameters on zooplankton distribution in major river systems of Sundarbans Mangrove | |
| James | A comparison of roe enhancement of the sea urchin Evechinus chloroticus in sea-based and land-based cages | |
| CN108064773A (en) | A kind of method that saline-alkali water raises and train colored perch juvenile fish | |
| Kirkpatrick et al. | Salinity tolerance and osmoregulation of a prawn, Palaemon affinis Milne Edwards (Caridea: Palaemonidae) | |
| CN105532524B (en) | A kind of grade classification and preferred method of the resistance to ammonia nitrogen performance of Marsupenaeus japonicus shrimp seedling | |
| Marin E et al. | Age, growth and fecundity of the silver mullet, Mugil curema (Pisces: Mugilidae), in coastal areas of Northeastern Venezuela | |
| Takahashi et al. | Variability in growth rates of Japanese jack mackerel Trachurus japonicus larvae and juveniles in the East China Sea–effects of temperature and prey abundance | |
| FOGARTY et al. | Metapopulation dynamics of coastal decapods | |
| Hutchings et al. | Life-history strategies of Umbrina robinsoni (Sciaenidae) in warm-temperate and subtropical South African marine reserves | |
| Yang et al. | Nonlethal sperm colleclion and cryopreservation in the eastern oyster Crassostrea virginica | |
| Rey et al. | Contrasting oceanographic conditions during larval development influence the benthic performance of a marine invertebrate with a bi-phasic life cycle | |
| Paulsen et al. | Growth rate and nutritional status of wild and released reared juvenile turbot in southern Kattegat, Denmark | |
| Warren-Myers et al. | Enriched stable isotope marking of hatchery trout via immersion: A method to monitor restocking success | |
| Hajji et al. | Reproductive biology of the black goby, Gobius niger (Teleostei: Gobiidae), in the Gulf of Gabes (Tunisia, Central Mediterranean) | |
| Johnston et al. | Life history variation within and among naturalized rainbow trout populations of the Laurentian Great Lakes | |
| CN105475184A (en) | Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method | |
| Ofor et al. | Effect of water hardness on fertilisation and hatching success of Clarias gariepinus (Burchell, 1822) and Heterobranchus longifilis Valenciennes, 1840 eggsfertilised with C. gariepinus sperm | |
| Charan-Dixon | Sea cucumber fisheries in the Kingdom of Tonga: regeneration biology, ecology, and environmental chemistry | |
| Ridho | Reproduction aspect of tiger fish (Datnioides polota hamilton) in musi river South Sumatera | |
| CN115152670B (en) | Domestication method of salt-tolerant mandarin fish fries | |
| Rizal et al. | Biology reproduction and its role in the conservation of goldband goatfish (Upeneus moluccensis (Bleeker, 1855)) in Rancabuaya Water, Indonesia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180525 |