CN105475184A - Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method - Google Patents
Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention discloses a lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method, and belongs to the technical field of aquaculture. According to the lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method, salinity is arranged to be 15-30 when a lateolabrax maculatus oosperm is hatched; and during a lateolabrax maculatus newly hatched larva period, the salinity is arranged to be 20-30, concentration of Ca<2+> in water is kept to be greater than 194.26 mg/L, and concentration of Mg<2+> in the water is kept to be greater than 556.67 mg/L. The lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method has the advantages that lateolabrax maculatus oosperm hatching rate is high, deformity rate is low and larva survival vitality is strong, and theoretical foundation is provided for performing lateolabrax maculatus oosperm hatching and early stage lateolabrax maculatus seedling desalination in estuary and saline-alkali soil regions.
Description
Technical field
The present invention relates to technical field of aquaculture, particularly relate to a kind of perch fertilized egg and newly hatched larvae spent and hatch and cultural method.
Background technology
Flower perch (Lateolabraxmaculatus) is under the jurisdiction of Osteichthyes (Osteichsthyes), (Perciformes), Sushi section (Serranidae), flower perch belong to (Lateolabrax) (Kim etc. to Perciformes, 2001), also known as spending perch in vain, perch fish, plate perch, cunner, seven-star perch, perch, stockaded village spend.Be distributed in China and Korea coastal, be Northeast Asia endemic species.
Bleeker in 1853 sets up flower perch and belongs to, and now this genus only has a kind, i.e. Lateolabraxjaponicus (CuvieretValenciennes).Nineteen fifty-seven, Katayama has found another kind that colored perch belongs to, i.e. high body perch L.latus.1995 ~ 1998 years, by Morphological comparison and genetic analysis, Yokogawa etc. find that Chinese sea bass and Japanese perch (L.japonicus) exist notable difference, and be divided into 2 kinds, the colored perch of China is called Lateolabraxsp. (spottedseabass).Calendar year 2001, Kim etc. when writing " ThemarinefishesofKorea " by Lateolabraxsp. (spottedseabass) called after L.maculatus.Liu Jinxian etc. (2006) utilize mitochondrial cytochrome b and Control region to carry out population structure analysis to Chinese sea bass and Lateolabrax japonicus shoal of fish body, Hu Zimin etc. (2007) utilize randomly amplified polymorphic DNA (RAPD) technology to carry out Genetic Differentiation to Chinese sea bass and Japanese perch, and it is 2 viewpoints not of the same race that acquired results supports Chinese sea bass and Japanese perch all further.
Flower perch is lower floor fish in the wide temperature of river mouth type, eurysalinity.Happiness inhabits river mouth salt-fresh water and to cross waters, and more with Ya Jiao flowing water district, does not form the habit of the long-range migration of jumpbogroup, but has the mobile phenomenon in local.Flower perch is predacious fish, and disposition is violent voracious, with fish, shrimp and shell-fish for food.Fast growth is had, the features such as meat is good in the Hekou Area of salt-fresh water, Low-salinity.Flower perch gonochorism, milter 2 partial maturation in age, 3 ages are all ripe, and raun 3 rheological properties is ripe, and 4 ages are all ripe.The reproduction water temperature of flower perch is at 13 ~ 21 DEG C, and Along The Coast of Shandong Province reproduction period is 9 ~ November, and optimum water temperature is 14 ~ 16 DEG C; The reproduction period of Fujian offshore is January in December to next year; The reproduction period of Japan perch is then November to next year 2, and optimum water temperature is 18 ~ 22 DEG C.In mating season, milter sexual gland is more first than raun ripe, and completes reproduction activity after can waiting for raun maturation.Flower perch belongs to the fish that in batches lay eggs, and can lay eggs 2 ~ 3 times in a breeding season, grows just can lay eggs for the second time after laying eggs for the first time through two weeks.Flower perch fertility is comparatively strong, absolute fish brood amount 5 ~ 2,300,000, and the colored perch fish brood amount of the long 500 ~ 600mm of body can reach 17.7 ~ 23.3 ten thousand, and the raun fish brood amount of the long 910mm of body can reach 209.4 ten thousand.The breeding waters of flower perch is distributed more widely, between shallow sea, the littoral cay in river mouth and the salt-fresh water intersection waters in nearly river mouth all can lay eggs.
Existing numerous scholar has carried out the research of colored perch artificial breeding technology aspect.Sun Guoying (1994), Zhang Chundan etc. (2005), (thank to phoenix just etc., 2012) the reproductive biology characteristic of colored perch is described from aspects such as Type of Spawning, egg-laying period, breeding waters, fertility and hermaphroditisms, and propose parent population selection, parent population domestication, accelerating cultivation, the correlation technique of artificial induced spawning and seed rearing.Wu Guangzong etc. (1984) describe the morphological feature of Bohai Sea Gulf flower perch early development stage, Li Mingyun etc. (1996,1997), Zhu Jun congruence (2000,2002) also reports morphological feature and the life habit in colored perch larva and juvenile stage.In salinity in the affecting of Lateolabrax japonicus embryo, Wang Yongxin etc. (1995), Hu Xiancheng etc. (1995,1997) have studied temperature, salinity respectively and grow Lateolabrax japonicus embryo and the impact of Larval.According to (1995) such as Wang Yongxins, flower perch fertilized egg is in the salinity range of 13 ~ 31, and flower perch all can hatch prelarva, but from incubation rate and abnormal rate, Low-salinity 13 ~ 16 and high salinity 31 unfavorable to growth.Being suitable for hatching salinity is 19 ~ 28, and the best is 22 ~ 25; Zhu Jun congruence (2002) report hatching salinity should control 26 ~ 27, and average percentage hatch rate reaches 74.3%; Li Mingyun etc. (1996) think that hatching optimal salinity is 27.2 ~ 29.8.Wang Yongxins etc. (1995) think that the salinity in the suitableeest hatching salinity and flower perch suitable waters is for a long time relevant.
Summary of the invention
The invention provides a kind of perch fertilized egg and newly hatched larvae spent to hatch and cultural method.
The present invention adopts following technical scheme:
Colored perch fertilized egg of the present invention and newly hatched larvae are hatched and cultural method, and when flower perch incubating oosperm, arranging salinity is 15 ~ 30; Flower perch newly hatched larvae period, arranging salinity is 20 ~ 30, and keeps Ca in water
2+concentration is greater than 194.26mg/L, Mg
2+concentration is greater than 556.67mg/L.
The adjustment of salinity is by preparing in proportion with seawater and running water.
Preferred: during flower perch incubating oosperm, arranging salinity is 20 ~ 30.
Preferred: flower perch newly hatched larvae period, arranging salinity is 25 ~ 30.
Good effect of the present invention is as follows:
To have colored perch hatchability of fertile eggs high for colored perch fertilized egg of the present invention and newly hatched larvae hatching and cultural method, the advantage that abnormal rate is low and prelarva survival activity is strong, for river mouth, area, saline land carry out spending perch incubating oosperm, early stage flower perch seedling desalination provides theoretical foundation.
Accompanying drawing explanation
Fig. 1 is the sink-float figure of flower perch fertilized egg in different salinity seawater.
Fig. 2 is the incubation rate and abnormal rate figure of spending perch fertilized egg under different salinity and ionic conditions;
Note: numerical value mean+SD represents, indicates the numeric representation significant difference (P<0.05) of different letter, otherwise represents difference not significantly (P > 0.05).
Fig. 3 is the SAI value figure of flower perch newly hatched larvae under different salinity and ionic conditions.
Embodiment
The following examples describe in further detail of the present invention.
Embodiment 1
1 experiment material
1.1 fertilized egg sources
Experiment is purchased from Guangdong Jiangmen aquatic farm with fertilized egg, for the parent population of ALONG COASTAL FUJIAN cage culture is supported temporarily through indoor, obtains after artificial induced spawning and insemination.Under water temperature 16 DEG C of conditions, fertilized egg is collected complete and is in primitive gut late period when being transported to Doumen District, Zhuhai City estuarine fishery research institute.Select normotrophic fertilized egg to test for salinity.
1.2 experimental water sources
Experiment seawater is Zhuhai coastal seawater, is transported to Doumen District, Zhuhai City estuarine fishery research institute by waterr transporting vehicle.Fresh water is the running water of waterworks, Zhuhai supply.Use CaCl respectively
2, MgCl
2, KCl (analyzing pure) adds potassium ion, calcium, magnesium ion in running water, calcium, magnesium, potassium ion to seawater concentration with obtain adding potassium running water, add the running water of calcium magnesium and add the running water of calcium magnesium potassium.Measure salinity with ATAGO hand-hold refractometer, with EDTA complexometric titration calcium, magnesium ion concentration, with sodium, the potassium concentration of Flame Atomic Absorption Spectrometry Determination, each experimental water ion concentration is as (table 1).Above experimental water is all through fully precipitating and testing for salinity after aeration.
2 experimental techniques
The sink-float test of fertilized egg under 2.1 different salinity conditions
Arrange 12 salinity gradients, its salinity is respectively: 20,21,22,23,23.4,24.3,25.2,26,27,28,29,30.Configure by a certain percentage with seawater and running water and obtain.Carry out the test of fertilized egg sink-float property with 500mL graduated cylinder, get 200, fertilized egg at every turn, after fertilized egg drops into graduated cylinder, stablize 10min, observe and record the existence of fertilized egg in different salinity seawater.
Table 1 experimental water ion concentration
Incubating oosperm experiment under 2.2 different salinity and ionic conditions
25,20,15,10,5 totally 5 salinity gradients are set, each salinity arrange A (normally), B (supplementing potassium ion to concentration of seawater), C (supplementing calcium, magnesium ion to concentration of seawater), D (supplementing calcium, magnesium, potassium ion to concentration of seawater) totally 4 different ion processing (wherein because B, D group Na of salinity 10 and 5
+: K
+lower, so do not arrange), seawater control group salinity is 30.Often group arrange 3 parallel, totally 51 polyethylene hatching cups.Wherein A process seawater and running water configure by a certain percentage; B process seawater configures by a certain percentage with the running water adding potassium; C process seawater configures by a certain percentage with the running water adding calcium magnesium; D process seawater configures by a certain percentage with the running water adding calcium magnesium potassium.Each processed group all demarcates salinity with ATAGO hand-hold refractometer, and its ion concentration is as (table 2).
Each processed group get 200 fertilized eggs put into 2000mL polyethylene hatching cup hatch, micro-inflation piles up death to avoid the fertilized egg in Low-salinity group to sink to the bottom, all processed group are placed in same 160cm × 70cm × 20cm square tank, hatching water temperature 16.5 ~ 17.5 DEG C is controlled with heating rod, pH7.9 ~ 8.0, indoor incandescent lamp keeps intensity of illumination to be 400 ~ 500LUX (Shanghai International Automobile City Tourist Festival student's federation industry JD-3 type illuminometer mensuration).After prelarva hatches, record incubation rate and newly hatched larvae abnormal rate (lopsided prelarva refer to afterbody bends, the individuality of spinal curvature).
Because the content of sodium ion in seawater is far above calcium, magnesium, potassium ion, this is tested in identical salinity not discuss and adds ion and the sodium ion total amount difference that causes, and the Na therefore caused only is discussed
+: K
+the change of ratio.
The survival activity test of newly hatched larvae under 2.3 different salinity and ionic conditions
From in the prelarva that hatches of each processed group of a upper joint experiment, the healthy newly hatched larvae of random selecting 50 tail put into corresponding salinity and ionic conditions 2000mL plastic cup (wherein remove healthy prelarva number extremely low 10A, 10C,
The ion concentration of table 2 different disposal group
5A, 5C group), cultivate without bait throwing in hydrostatic, draw dead prelarva every day 2 times (close tactile unresponsive for dead with glass bar), record dead prelarva number, during hungry death whole in prelarva, experiment terminates.Calculate newly hatched larvae survival activity (survivalactivityindex, SAI).
2.4 index determining
Testing index in this research mainly comprises incubation rate, abnormal rate, SAI value.
Incubation rate=rupture of membranes prelarva number/hatch total ovum number × 100%;
Abnormal rate=lopsided prelarva number/rupture of membranes prelarva number × 100%;
Prelarva number when N is on-test in formula, the accumulation death toll of prelarva when hi is i-th day, the number of days of k required for the whole death of prelarva.
2.5 data analysis
Use SPSS statistical software (19.0) to carry out ANOVA one-way analysis of variance to incubation rate, abnormal rate, SAI value, and carry out DUNCAN Multiple range test, with P < 0.05 for there being significant difference; Two-way analysis of variance is carried out to SAI value.All data mean ± standard deviation represents (Mean ± SD), finally maps with EXCEL2013.
3 results
The sink-float of flower perch fertilized egg under 3.1 different salinity conditions
The distribution of fertilized egg in different salinity seawater is different.Salinity lower than 23 time, fertilized egg all sink to bottom; When salinity is greater than 27, all float on water surface; Salinity is 23.4 ~ 26 time, and fertilized egg has part to suspend in water, is wherein all suspensions (Fig. 1) when salinity is 25.
3.2 different salinity become incubation rate and the abnormal rate of flower perch fertilized egg under condition with ion set
Fig. 2 shows the impact of salinity and the paired hatchability of fertile eggs of ion set and abnormal rate.The average percentage hatch rate of salinity 15 ~ 30 processed group is all greater than 90%; Salinity 10 processed group average percentage hatch rate is greater than 80%, and salinity 5 processed group average percentage hatch rate is 5.3%, but the newly hatched larvae of salinity 5 and 10 processed group is all dead in 24h after rupture of membranes; In same salinity, the paired incubation rate of ion set does not have a significant impact (P > 0.05).In the processed group of salinity 20 ~ 30, abnormal rate is all lower than 5%; The processed group of salinity 5, abnormal rate average out to 97.5%; The abnormal rate of salinity 10 ~ 15 processed group is 8.4% ~ 33.4%, in same salinity, the paired abnormal rate of ion set has a significant impact (P < 0.05), wherein the abnormal rate of B (supplementing potassium ion group) is significantly higher than A (normal group) and D (supplementing calcium, magnesium, potassium ion group), and the abnormal rate of C (supplementing calcium, magnesium ion group) is significantly lower than A and D process (P < 0.05).
The SAI value of flower perch newly hatched larvae under 3.3 different salinity and ionic conditions
The interaction of salinity, ion composition, salinity and ion composition all has pole significance impact (P < 0.01) (Fig. 3 and table 4) to the SAI value of newly hatched larvae.Salinity 30 and 25 processed group SAI value is maximum, and in salinity 15 ~ 25 processed group, SAI value reduces along with the reduction of salinity.The processed group intermediate ion composition of salinity 25 is not on the impact of SAI value significantly (P > 0.05); In salinity 15 and 20 processed group, the paired SAI value of ion set has a significant impact (P < 0.05).In the processed group of wherein salinity 20, the SAI value of A (normal group) and C (supplementing calcium, magnesium ion group) is higher than B (supplementing potassium ion group) and D (supplementary calcium, magnesium, potassium ion group); In the processed group of salinity 15, the SAI value of C group is the highest, the SAI value minimum (table 3) of B and D group.
Table 4 two-way analysis of variance salinity and ion set spend the impact of perch newly hatched larvae SAI value in pairs
The present invention find salinity lower than 23 time, flower perch fertilized egg all sinks to bottom water layer; When salinity is greater than 27, all float on water layer surface; Salinity is 23.4 ~ 26 time, and fertilized egg has part to suspend in water.It is 15 ~ 30 that flower perch fertilized egg is suitable for hatching salinity, and best hatching salinity is 20 ~ 30; The suitable salinity scope of flower perch newly hatched larvae is 20 ~ 30, and optimal salinity scope is 25 ~ 30.Work as Ca
2+≤ 194.26mg/L, Mg
2+the abnormal rate of colored perch incubating oosperm can be increased during≤556.67mg/L, reduce the vigor of prelarva.
Although illustrate and describe embodiments of the invention, for the ordinary skill in the art, be appreciated that and can carry out multiple change, amendment, replacement and modification to these embodiments without departing from the principles and spirit of the present invention, scope of the present invention is by claims and equivalents thereof.
Claims (4)
1. spend perch fertilized egg and newly hatched larvae to hatch and a cultural method, it is characterized in that: when flower perch incubating oosperm, arranging salinity is 15 ~ 30; Flower perch newly hatched larvae period, arranging salinity is 20 ~ 30, and keeps Ca in water
2+concentration is greater than 194.26mg/L, Mg
2+concentration is greater than 556.67mg/L.
2. flower perch fertilized egg as claimed in claim 1 and newly hatched larvae are hatched and cultural method, it is characterized in that: the adjustment of salinity is by preparing in proportion with seawater and running water.
3. flower perch fertilized egg as claimed in claim 1 and newly hatched larvae are hatched and cultural method, it is characterized in that: during flower perch incubating oosperm, arranging salinity is 20 ~ 30.
4. flower perch fertilized egg as claimed in claim 1 and newly hatched larvae hatching and cultural method, is characterized in that: flower perch newly hatched larvae period, and arranging salinity is 25 ~ 30.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510799023.8A CN105475184A (en) | 2015-11-18 | 2015-11-18 | Lateolabrax maculatus oosperm and newly hatched larva hatching and cultivation method |
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| Application Number | Priority Date | Filing Date | Title |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108064773A (en) * | 2017-12-21 | 2018-05-25 | 中国海洋大学 | A kind of method that saline-alkali water raises and train colored perch juvenile fish |
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| CN108064773A (en) * | 2017-12-21 | 2018-05-25 | 中国海洋大学 | A kind of method that saline-alkali water raises and train colored perch juvenile fish |
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