CN105403545B - 三维中的亚衍射极限图像分辨率 - Google Patents

三维中的亚衍射极限图像分辨率 Download PDF

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CN105403545B
CN105403545B CN201510702700.XA CN201510702700A CN105403545B CN 105403545 B CN105403545 B CN 105403545B CN 201510702700 A CN201510702700 A CN 201510702700A CN 105403545 B CN105403545 B CN 105403545B
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light
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image
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CN105403545A (zh
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庄小威
黄波
维尔弗雷德·M·巴泰斯
王文芹
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Harvard University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N13/00Stereoscopic video systems; Multi-view video systems; Details thereof
    • H04N13/20Image signal generators
    • H04N13/204Image signal generators using stereoscopic image cameras
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458Fluorescence microscopy
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/16Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/36Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361Optical details, e.g. image relay to the camera or image sensor
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/36Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365Control or image processing arrangements for digital or video microscopes
    • G02B21/367Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58Optics for apodization or superresolution; Optical synthetic aperture systems
    • HELECTRICITY
    • H04ELECTRIC COMMUNICATION TECHNIQUE
    • H04NPICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N7/00Television systems
    • H04N7/18Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6419Excitation at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6421Measuring at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Signal Processing (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CN201510702700.XA 2007-12-21 2008-12-19 三维中的亚衍射极限图像分辨率 Active CN105403545B (zh)

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Publication number Priority date Publication date Assignee Title
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
DE102007045897A1 (de) * 2007-09-26 2009-04-09 Carl Zeiss Microimaging Gmbh Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe
WO2009085218A1 (en) * 2007-12-21 2009-07-09 President And Fellows Of Harvard College Sub-diffraction limit image resolution in three dimensions
US8693742B2 (en) 2008-12-17 2014-04-08 The Regents Of The University Of Colorado Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function
EP2449379B1 (en) 2009-07-02 2017-05-17 Sloan-Kettering Institute for Cancer Research Fluorescent silica-based nanoparticles
US12161734B2 (en) 2009-07-02 2024-12-10 Sloan-Kettering Institute For Cancer Research Multimodal silica-based nanoparticles
CN102574405B (zh) * 2009-10-16 2014-11-05 巴斯夫欧洲公司 具有窄带的标记物
DE102009060793A1 (de) * 2009-12-22 2011-07-28 Carl Zeiss Microlmaging GmbH, 07745 Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten
EP2372347A1 (en) * 2010-03-26 2011-10-05 Institut Pasteur Method to increase the number of detectable photons during the imaging of a biological marker
US8620065B2 (en) * 2010-04-09 2013-12-31 The Regents Of The University Of Colorado Methods and systems for three dimensional optical imaging, sensing, particle localization and manipulation
WO2011152523A1 (ja) * 2010-06-03 2011-12-08 株式会社ニコン 顕微鏡装置
DE102010036709A1 (de) 2010-07-28 2012-02-02 Leica Microsystems Cms Gmbh Einrichtung und Verfahren zur mikroskopischen Bildaufnahme einer Probenstruktur
WO2012039636A2 (en) * 2010-09-24 2012-03-29 Christian Soeller "3d localisation microscopy and 4d localisation microscopy and tracking methods and systems"
US10024796B2 (en) 2010-10-29 2018-07-17 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
DE102010044013A1 (de) 2010-11-16 2012-05-16 Carl Zeiss Microimaging Gmbh Tiefenauflösungsgesteigerte Mikroskopie
FR2978254B1 (fr) 2011-07-21 2014-01-24 Imagine Optic Methode et dispositif optique pour la localisation d'une particule en super resolution
JP6042887B2 (ja) * 2011-07-24 2016-12-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ポリクロイックミラーチェンジャー付き蛍光顕微鏡
EP2737356A1 (en) * 2011-07-25 2014-06-04 Mad City Labs, Inc. Active-feedback positional drift correction in a microscope image using a fiduciary element held on a nanopositioning stage
DE102011053232B4 (de) 2011-09-02 2020-08-06 Leica Microsystems Cms Gmbh Mikroskopische Einrichtung und mikroskopisches Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten
US10001622B2 (en) * 2011-10-25 2018-06-19 Sanford Burnham Medical Research Institute Multifunction autofocus system and method for automated microscopy
DE102011055294B4 (de) * 2011-11-11 2013-11-07 Leica Microsystems Cms Gmbh Mikroskopische Einrichtung und Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten in einer Probe
US9291562B2 (en) * 2011-11-15 2016-03-22 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Method and apparatus for tracking a particle, particularly a single molecule, in a sample
US9693034B2 (en) 2011-12-13 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University Apparatus and method for localizing objects for distance and/or in three dimensions using a spiral point spread function
WO2013090360A2 (en) 2011-12-15 2013-06-20 President And Fellows Of Harvard College High resolution dual-objective microscopy
JP2013152429A (ja) * 2011-12-27 2013-08-08 Canon Inc 画像処理装置、画像処理方法、およびプログラム
CN102565795B (zh) * 2011-12-30 2014-01-15 北京华航无线电测量研究所 一种圆柱阵面三维成像系统的多角度成像显示方法
CN102520408B (zh) * 2011-12-30 2013-07-03 北京华航无线电测量研究所 一种圆柱阵面三维成像系统的三维成像方法
CN102565796B (zh) * 2011-12-30 2013-09-18 北京华航无线电测量研究所 一种圆柱阵面三维成像系统的成像方法
DE102012200344A1 (de) * 2012-01-11 2013-07-11 Carl Zeiss Microscopy Gmbh Mikroskopsystem und Verfahren für die 3-D hochauflösende Mikroskopie
EP2660639B1 (en) * 2012-05-02 2016-04-13 Centre National De La Recherche Scientifique Method and apparatus for single-particle localization using wavelet analysis
US10955331B2 (en) 2012-06-22 2021-03-23 The Regents Of The University Of Colorado, A Body Corporate Imaging or measurement methods and systems
US20140022373A1 (en) * 2012-07-20 2014-01-23 University Of Utah Research Foundation Correlative drift correction
JP6131568B2 (ja) * 2012-10-30 2017-05-24 株式会社ニコン 顕微鏡装置及び画像形成方法
US9717402B2 (en) * 2012-11-21 2017-08-01 Align Technology, Inc. Confocal imaging using astigmatism
US9063434B2 (en) 2013-01-11 2015-06-23 University Of Utah Research Foundation Sub-diffraction-limited patterning and imaging via multi-step photoswitching
JP6349091B2 (ja) * 2013-01-18 2018-06-27 国立大学法人 東京大学 超解像蛍光イメージング用プローブ
WO2014144820A1 (en) 2013-03-15 2014-09-18 The Regents Of The University Of Colorado 3-d localization and imaging of dense arrays of particles
WO2014145606A1 (en) 2013-03-15 2014-09-18 Sloan-Kettering Institute For Cancer Research Multimodal silica-based nanoparticles
DE102013102988A1 (de) * 2013-03-22 2014-09-25 Leica Microsystems Cms Gmbh Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten
US9435993B2 (en) 2013-03-24 2016-09-06 Bruker Nano, Inc. Three dimensional microscopy imaging
DE102013208926A1 (de) 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013208927A1 (de) * 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013106895B4 (de) * 2013-07-01 2015-09-17 Leica Microsystems Cms Gmbh Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten
US10036735B2 (en) 2013-08-26 2018-07-31 The Regents Of The University Of Colorado, A Body Corporate Imaging through scattering media with high signal to noise ratio and resolution
DE102013015933A1 (de) 2013-09-19 2015-03-19 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie
WO2015046440A1 (ja) 2013-09-27 2015-04-02 株式会社ニコン 解析装置、顕微鏡装置、解析方法、及びプログラム
CN104677865B (zh) * 2013-12-02 2018-03-20 大连光耀辉科技有限公司 一种显微分析装置
JP6284372B2 (ja) * 2014-01-21 2018-02-28 オリンパス株式会社 走査型レーザ顕微鏡および超解像画像生成方法
US8977024B1 (en) * 2014-01-31 2015-03-10 Afraxis, Inc. Distributed anatomical image analysis
KR102313982B1 (ko) 2014-03-11 2021-10-18 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 프로그램가능한 핵산 프로브를 이용한 고처리량 고도 다중 영상화
US9810893B2 (en) 2014-03-27 2017-11-07 The Board Of Trustees Of The Leland Stanford Junior University Phase mask imaging with characterization of rotational mobility
US20150302884A1 (en) * 2014-04-18 2015-10-22 Battelle Memorial Institute Apparatus and method of multi-bit and gray-scale high density data storage
RU2016144055A (ru) 2014-05-29 2018-07-02 Мемориал Слоун Кеттеринг Кэнсэ Сентр Конъюгаты лекарственного средства с наночастицами
ES2968004T3 (es) 2014-07-30 2024-05-06 Harvard College Construcción de bibliotecas de sondas
US20160069903A1 (en) 2014-09-10 2016-03-10 Fundació Institute De Ciències Foròniques Method for detecting cells
EP3215806A4 (en) 2014-11-05 2018-06-06 The Regents Of The University Of Colorado 3d imaging, ranging, and/or tracking using active illumination and point spread function engineering
EP3237890A4 (en) * 2014-12-16 2018-11-07 President and Fellows of Harvard College Triggered assembly of metafluorophores
US9921161B1 (en) * 2015-01-08 2018-03-20 Daniel Feldkhun Structured light active localization microscopy
JP6628969B2 (ja) 2015-03-06 2020-01-15 シスメックス株式会社 細胞分析装置、細胞分析装置の制御方法およびプログラム
WO2016157346A1 (ja) * 2015-03-27 2016-10-06 株式会社ニコン 顕微鏡装置、観察方法、及び制御プログラム
DE102015105018A1 (de) * 2015-03-31 2016-10-06 Laser-Laboratorium Göttingen e.V. Verfahren und Rasterfluoreszenzlichtmikroskop zum mehrdimensional hochauflösenden Abbilden einer Struktur oder eines Wegs eines Partikels in einer Probe
US10341640B2 (en) 2015-04-10 2019-07-02 The Board Of Trustees Of The Leland Stanford Junior University Multi-wavelength phase mask
CA2991920C (en) * 2015-04-23 2024-05-14 The University Of British Columbia Multifocal method and apparatus for stabilization of optical systems
CA2985126A1 (en) 2015-05-29 2016-12-08 Cornell University Methods of treatment using ultrasmall nanoparticles to induce cell death of nutrient-deprived cancer cells via ferroptosis
WO2017090211A1 (ja) * 2015-11-27 2017-06-01 株式会社ニコン 顕微鏡装置
KR101766064B1 (ko) * 2015-11-30 2017-08-08 연세대학교 산학협력단 내부 전반사 형광 현미경
US10783697B2 (en) 2016-02-26 2020-09-22 Yale University Systems, methods, and computer-readable media for ultra-high resolution 3D imaging of whole cells
JP2019527466A (ja) * 2016-06-14 2019-09-26 エバナ テクノロジーズ ユーエービー 多分割レンズ及びウェハをダイシングまたは切断するためのレーザー加工システム
JP6423841B2 (ja) 2016-10-11 2018-11-14 浜松ホトニクス株式会社 試料観察装置及び試料観察方法
EP3539036A4 (en) 2016-11-08 2020-06-17 President and Fellows of Harvard College MATRIX AND DELETE
US20190276881A1 (en) 2016-11-08 2019-09-12 President And Fellows Of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
DE102017211031A1 (de) 2016-11-21 2018-05-24 Carl Zeiss Microscopy Gmbh Verfahren und Mikroskop zum Ermitteln einer Fluoreszenzintensität
US11067510B2 (en) * 2016-11-29 2021-07-20 University of Pittsburgh—of the Commonwealth System of Higher Education System and method for estimating and compensating for sample drift during data acquisition in fluorescence microscopy
WO2018107054A1 (en) 2016-12-09 2018-06-14 Ultivue, Inc. Improved methods for multiplex imaging using labeled nucleic acid imaging agents
CN106772975B (zh) * 2017-01-19 2019-08-13 宁波纳美致生物科技有限公司 针对细胞或组织不同深度超分辨率显微成像的照明系统
JP2020520953A (ja) 2017-05-25 2020-07-16 メモリアル スローン ケタリング キャンサー センター ジルコニウム−89で標識した超小型ナノ粒子およびその方法
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
DE102017111578A1 (de) 2017-05-29 2018-11-29 Wladimir Schaufler Verfahren zur Unterscheidung einzelner fluoreszierender Markierungsstoffmoleküle in der SPDM-Lokalisationsmikroskopie durch deren zeitliches Langzeit-Emissionsverhalten über 10ms
EP3418391B1 (en) 2017-06-23 2022-09-28 Fundació Institut de Ciències Fotòniques Method for quantifying protein copy-number
EP3642599B1 (en) * 2017-06-23 2025-01-08 Memorial Sloan-Kettering Cancer Center Method of imaging in vivo tissues using nanoparticles comprising a reference dye and a sensor dye.
KR20240042123A (ko) * 2017-08-30 2024-04-01 바이오액시알 사스 단일 분포 및 딥 러닝에 기초한 초해상도 계측 방법
JP7228917B2 (ja) * 2018-01-02 2023-02-27 キングス カレッジ ロンドン 局在化顕微鏡法のための方法及びシステム
CN113366116A (zh) 2018-12-13 2021-09-07 哈佛学院院长及董事 用于merfish和其他应用的扩增方法和系统
CN111323397B (zh) * 2018-12-14 2023-03-28 深圳华大生命科学研究院 光学成像系统、成像检测系统与方法及基因测序方法
US11255785B2 (en) * 2019-03-14 2022-02-22 Applied Materials, Inc. Identifying fiducial markers in fluorescence microscope images
US11469075B2 (en) 2019-03-14 2022-10-11 Applied Materials, Inc. Identifying fiducial markers in microscope images
DE102019108696B3 (de) 2019-04-03 2020-08-27 Abberior Instruments Gmbh Verfahren zum Erfassen von Verlagerungen einer Probe gegenüber einem Objektiv
CA3161149A1 (en) 2019-11-20 2021-05-27 President And Fellows Of Harvard College Methods for multi-focal imaging for molecular profiling
IL273118B (en) 2020-03-05 2022-03-01 Allen Richter Self-adaptive electromagnetic radiation guide
KR102619825B1 (ko) * 2020-10-20 2024-01-03 한양대학교 산학협력단 초고해상도 형광 현미경용 이미징 버퍼 용액 및 이를 이용한 초고해상도 형광 현미경 이미징 방법
KR102654655B1 (ko) * 2020-10-20 2024-04-05 한양대학교 산학협력단 초고해상도 형광 현미경 이미징 방법 및 초고해상도 형광 현미경 이미징 장치
WO2022102584A1 (ja) * 2020-11-16 2022-05-19 株式会社ニコン 顕微鏡
CN119693238A (zh) * 2025-02-27 2025-03-25 深圳市真迈生物科技有限公司 提升图像分辨率的方法、系统及装置
TWI905063B (zh) * 2025-06-04 2025-11-11 優立測科技股份有限公司 高速光學元件對位系統與方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4549807A (en) * 1983-10-07 1985-10-29 At&T Bell Laboratories Process for measuring fluorescence
CN1779436A (zh) * 2004-11-26 2006-05-31 上海爱建纳米科技发展有限公司 用于原子力显微镜的同轴照明显微镜光学系统
WO2006123967A2 (en) * 2005-05-18 2006-11-23 Andrey Alexeevich Klimov Fluorescent nanoscopy method
CN1963469A (zh) * 2006-11-28 2007-05-16 南开大学 超短脉冲激光诱导的多光子电离非线性显微成像方法和系统及其应用

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1252408A (en) 1916-09-25 1918-01-08 Willard Daubenberg Attachment for submarines.
US5736410A (en) 1992-09-14 1998-04-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
JP4812937B2 (ja) * 1998-03-16 2011-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 共焦点顕微鏡イメージングシステム
AU6842401A (en) 2000-06-14 2001-12-24 Clontech Lab Inc Fluorescent timer proteins and methods for their use
JP2002062261A (ja) 2000-08-21 2002-02-28 Olympus Optical Co Ltd 光学装置および顕微鏡
US6844150B2 (en) 2000-08-24 2005-01-18 The Regents Of The University Of California Ultrahigh resolution multicolor colocalization of single fluorescent probes
US7309568B2 (en) 2001-09-26 2007-12-18 Hitachi, Ltd. Method in inspecting DNA and apparatus therefor
US7430045B2 (en) 2003-04-13 2008-09-30 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. High spatial resolution imaging
JP5414147B2 (ja) 2003-04-13 2014-02-12 マックス−プランク−ゲゼルシヤフト・ツーア・フェルデルング・デア・ヴィッセンシャフテン・アインゲトラーゲナー・フェライン 立体的高解像度結像
US7116590B2 (en) * 2004-08-23 2006-10-03 Micron Technology, Inc. Memory address repair without enable fuses
US7054051B1 (en) 2004-11-26 2006-05-30 Alces Technology, Inc. Differential interferometric light modulator and image display device
EP2453238B1 (en) 2005-05-23 2016-12-21 Harald F. Hess Optical microscopy with phototransformable optical labels
JP4779518B2 (ja) 2005-09-08 2011-09-28 パナソニック株式会社 蛍光読取装置および微生物計数装置
JP4572162B2 (ja) 2005-11-22 2010-10-27 株式会社ジーオングストローム 顕微鏡装置
US7564544B2 (en) * 2006-03-22 2009-07-21 3i Systems Corporation Method and system for inspecting surfaces with improved light efficiency
DE102006021317B3 (de) 2006-05-06 2007-10-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe
US7776613B2 (en) 2006-08-07 2010-08-17 President And Fellows Of Harvard College Sub-diffraction image resolution and other imaging techniques
US7838302B2 (en) 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
JP4609418B2 (ja) 2006-11-15 2011-01-12 トヨタ自動車株式会社 シフト切換機構の制御装置および制御方法
US7924432B2 (en) 2006-12-21 2011-04-12 Howard Hughes Medical Institute Three-dimensional interferometric microscopy
US20080241843A1 (en) * 2006-12-21 2008-10-02 Stanford University Single-cell analysis systems, methods of counting molecules in a single-cell, cylindrical fluorescence detection systems
US8217992B2 (en) 2007-01-11 2012-07-10 The Jackson Laboratory Microscopic imaging techniques
KR101165650B1 (ko) 2007-01-30 2012-07-17 에프. 포스잣 후, 엘.엘.씨. 이미지 전달 장치
EP2012173A3 (en) 2007-07-03 2009-12-09 JDS Uniphase Corporation Non-etched flat polarization-selective diffractive optical elements
WO2009085218A1 (en) 2007-12-21 2009-07-09 President And Fellows Of Harvard College Sub-diffraction limit image resolution in three dimensions
US7772569B2 (en) * 2008-04-01 2010-08-10 The Jackson Laboratory 3D biplane microscopy
US8248609B2 (en) * 2008-11-04 2012-08-21 The Johns Hopkins University Cylindrical illumination confocal spectroscopy system
DE102008054438A1 (de) 2008-12-09 2010-06-24 Seereal Technologies S.A. Optisches Bauteil zum Ablenken von das optische Bauteil durchlaufende Lichtstrahlen
US8879070B2 (en) 2009-06-11 2014-11-04 University Of Tsukuba Two beams formed by Wollaston prism in sample arm in an optical coherence tomography apparatus
US8101129B2 (en) 2009-10-13 2012-01-24 Chuang Shu-Yuan Nano filter structure for breathing and manufacturing method thereof
US9492322B2 (en) 2009-11-16 2016-11-15 Alcon Lensx, Inc. Imaging surgical target tissue by nonlinear scanning
WO2012039636A2 (en) 2010-09-24 2012-03-29 Christian Soeller "3d localisation microscopy and 4d localisation microscopy and tracking methods and systems"
US20140333750A1 (en) 2011-12-15 2014-11-13 President And Fellows Of Harvard College High resolution dual-objective microscopy
CA2869359C (en) 2012-04-03 2021-03-23 University Court Of The University Of St Andrews High resolution imaging of extended volumes
WO2015117115A1 (en) 2014-02-03 2015-08-06 President And Fellows Of Harvard College Three-dimensional super-resolution fluorescence imaging using airy beams and other techniques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4549807A (en) * 1983-10-07 1985-10-29 At&T Bell Laboratories Process for measuring fluorescence
CN1779436A (zh) * 2004-11-26 2006-05-31 上海爱建纳米科技发展有限公司 用于原子力显微镜的同轴照明显微镜光学系统
WO2006123967A2 (en) * 2005-05-18 2006-11-23 Andrey Alexeevich Klimov Fluorescent nanoscopy method
CN1963469A (zh) * 2006-11-28 2007-05-16 南开大学 超短脉冲激光诱导的多光子电离非线性显微成像方法和系统及其应用

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