JP2014066734A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014066734A5 JP2014066734A5 JP2014002163A JP2014002163A JP2014066734A5 JP 2014066734 A5 JP2014066734 A5 JP 2014066734A5 JP 2014002163 A JP2014002163 A JP 2014002163A JP 2014002163 A JP2014002163 A JP 2014002163A JP 2014066734 A5 JP2014066734 A5 JP 2014066734A5
- Authority
- JP
- Japan
- Prior art keywords
- light
- entities
- entity
- switchable
- image
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 claims description 166
- 230000003213 activating effect Effects 0.000 claims description 43
- 230000004913 activation Effects 0.000 claims description 19
- 238000000926 separation method Methods 0.000 claims description 19
- 230000005284 excitation Effects 0.000 claims description 12
- 230000003287 optical effect Effects 0.000 claims description 12
- 238000012937 correction Methods 0.000 claims description 7
- 230000000415 inactivating effect Effects 0.000 claims description 7
- 201000009310 astigmatism Diseases 0.000 claims description 6
- 230000009849 deactivation Effects 0.000 claims description 3
- 238000003384 imaging method Methods 0.000 description 93
- 239000000523 sample Substances 0.000 description 45
- 210000004027 cell Anatomy 0.000 description 37
- 108010090804 Streptavidin Proteins 0.000 description 27
- 239000011324 bead Substances 0.000 description 27
- 230000006870 function Effects 0.000 description 25
- 230000000670 limiting effect Effects 0.000 description 16
- 239000011521 glass Substances 0.000 description 15
- 239000000975 dye Substances 0.000 description 13
- 102000029749 Microtubule Human genes 0.000 description 12
- 108091022875 Microtubule Proteins 0.000 description 12
- 230000027455 binding Effects 0.000 description 12
- 239000000872 buffer Substances 0.000 description 12
- 210000004688 microtubule Anatomy 0.000 description 12
- 238000009826 distribution Methods 0.000 description 11
- 238000011088 calibration curve Methods 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 235000018102 proteins Nutrition 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- JLDSMZIBHYTPPR-UHFFFAOYSA-N Alexa Fluor 405 Chemical group CC[NH+](CC)CC.CC[NH+](CC)CC.CC[NH+](CC)CC.C12=C3C=4C=CC2=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C1=CC=C3C(S(=O)(=O)[O-])=CC=4OCC(=O)N(CC1)CCC1C(=O)ON1C(=O)CCC1=O JLDSMZIBHYTPPR-UHFFFAOYSA-N 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 239000006059 cover glass Substances 0.000 description 7
- 239000002096 quantum dot Substances 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 238000013480 data collection Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 239000000835 fiber Substances 0.000 description 6
- 238000000799 fluorescence microscopy Methods 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 102000005853 Clathrin Human genes 0.000 description 5
- 108010019874 Clathrin Proteins 0.000 description 5
- 239000012190 activator Substances 0.000 description 5
- 239000012472 biological sample Substances 0.000 description 5
- 229930193282 clathrin Natural products 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000010166 immunofluorescence Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 5
- QGKMIGUHVLGJBR-UHFFFAOYSA-M (4z)-1-(3-methylbutyl)-4-[[1-(3-methylbutyl)quinolin-1-ium-4-yl]methylidene]quinoline;iodide Chemical compound [I-].C12=CC=CC=C2N(CCC(C)C)C=CC1=CC1=CC=[N+](CCC(C)C)C2=CC=CC=C12 QGKMIGUHVLGJBR-UHFFFAOYSA-M 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003086 colorant Substances 0.000 description 4
- 238000005314 correlation function Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000002441 reversible effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000012103 Alexa Fluor 488 Substances 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 238000001493 electron microscopy Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 108091006047 fluorescent proteins Proteins 0.000 description 3
- 102000034287 fluorescent proteins Human genes 0.000 description 3
- 238000010569 immunofluorescence imaging Methods 0.000 description 3
- 239000003446 ligand Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 238000009827 uniform distribution Methods 0.000 description 3
- 239000012114 Alexa Fluor 647 Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 150000001412 amines Chemical group 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000004397 blinking Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000010191 image analysis Methods 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 239000010954 inorganic particle Substances 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 230000002186 photoactivation Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 229920002379 silicone rubber Polymers 0.000 description 2
- 239000004945 silicone rubber Substances 0.000 description 2
- 229910000033 sodium borohydride Inorganic materials 0.000 description 2
- 239000012279 sodium borohydride Substances 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000269350 Anura Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000867607 Chlorocebus sabaeus Species 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 1
- 229920002274 Nalgene Polymers 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- GLEVLJDDWXEYCO-UHFFFAOYSA-N Trolox Chemical compound O1C(C)(C(O)=O)CCC2=C1C(C)=C(C)C(O)=C2C GLEVLJDDWXEYCO-UHFFFAOYSA-N 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 102000012883 Tumor Necrosis Factor Ligand Superfamily Member 14 Human genes 0.000 description 1
- 108010065158 Tumor Necrosis Factor Ligand Superfamily Member 14 Proteins 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 102000035181 adaptor proteins Human genes 0.000 description 1
- 108091005764 adaptor proteins Proteins 0.000 description 1
- 125000003172 aldehyde group Chemical group 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 208000036815 beta tubulin Diseases 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000010226 confocal imaging Methods 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 230000012202 endocytosis Effects 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000013101 initial test Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000008606 intracellular interaction Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000010859 live-cell imaging Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000004651 near-field scanning optical microscopy Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000985 reactive dye Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000005469 synchrotron radiation Effects 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US866107P | 2007-12-21 | 2007-12-21 | |
| US61/008,661 | 2007-12-21 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010539499A Division JP5526036B2 (ja) | 2007-12-21 | 2008-12-19 | 三次元の回折限界未満の画像解像技術 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014149455A Division JP2014199260A (ja) | 2007-12-21 | 2014-07-23 | 三次元の回折限界未満の画像解像技術 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014066734A JP2014066734A (ja) | 2014-04-17 |
| JP2014066734A5 true JP2014066734A5 (enExample) | 2014-09-04 |
Family
ID=40506438
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010539499A Active JP5526036B2 (ja) | 2007-12-21 | 2008-12-19 | 三次元の回折限界未満の画像解像技術 |
| JP2014002163A Withdrawn JP2014066734A (ja) | 2007-12-21 | 2014-01-09 | 三次元の回折限界未満の画像解像技術 |
| JP2014149455A Withdrawn JP2014199260A (ja) | 2007-12-21 | 2014-07-23 | 三次元の回折限界未満の画像解像技術 |
| JP2016223789A Active JP6452663B2 (ja) | 2007-12-21 | 2016-11-17 | 三次元の回折限界未満の画像解像技術 |
| JP2018231326A Pending JP2019070820A (ja) | 2007-12-21 | 2018-12-11 | 三次元の回折限界未満の画像解像技術 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010539499A Active JP5526036B2 (ja) | 2007-12-21 | 2008-12-19 | 三次元の回折限界未満の画像解像技術 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014149455A Withdrawn JP2014199260A (ja) | 2007-12-21 | 2014-07-23 | 三次元の回折限界未満の画像解像技術 |
| JP2016223789A Active JP6452663B2 (ja) | 2007-12-21 | 2016-11-17 | 三次元の回折限界未満の画像解像技術 |
| JP2018231326A Pending JP2019070820A (ja) | 2007-12-21 | 2018-12-11 | 三次元の回折限界未満の画像解像技術 |
Country Status (5)
| Country | Link |
|---|---|
| US (6) | US8564792B2 (enExample) |
| EP (2) | EP2232244A1 (enExample) |
| JP (5) | JP5526036B2 (enExample) |
| CN (2) | CN101918816B (enExample) |
| WO (1) | WO2009085218A1 (enExample) |
Families Citing this family (93)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7838302B2 (en) | 2006-08-07 | 2010-11-23 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| DE102007045897A1 (de) * | 2007-09-26 | 2009-04-09 | Carl Zeiss Microimaging Gmbh | Verfahren zur mikroskopischen dreidimensionalen Abbildung einer Probe |
| CN101918816B (zh) | 2007-12-21 | 2015-12-02 | 哈佛大学 | 三维中的亚衍射极限图像分辨率 |
| US8693742B2 (en) | 2008-12-17 | 2014-04-08 | The Regents Of The University Of Colorado | Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function |
| US12161734B2 (en) | 2009-07-02 | 2024-12-10 | Sloan-Kettering Institute For Cancer Research | Multimodal silica-based nanoparticles |
| EP3499233A3 (en) | 2009-07-02 | 2019-09-18 | Sloan-Kettering Institute for Cancer Research | Fluorescent silica-based nanoparticles |
| US8610893B2 (en) * | 2009-10-16 | 2013-12-17 | Basf Se | Marking agents having narrow bands |
| DE102009060793A1 (de) * | 2009-12-22 | 2011-07-28 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Verfahren zur zwei- oder dreidimensionalen Positionsbestimmung von Objekten |
| EP2372347A1 (en) * | 2010-03-26 | 2011-10-05 | Institut Pasteur | Method to increase the number of detectable photons during the imaging of a biological marker |
| US8620065B2 (en) | 2010-04-09 | 2013-12-31 | The Regents Of The University Of Colorado | Methods and systems for three dimensional optical imaging, sensing, particle localization and manipulation |
| JP5459399B2 (ja) * | 2010-06-03 | 2014-04-02 | 株式会社ニコン | 顕微鏡装置 |
| DE102010036709A1 (de) * | 2010-07-28 | 2012-02-02 | Leica Microsystems Cms Gmbh | Einrichtung und Verfahren zur mikroskopischen Bildaufnahme einer Probenstruktur |
| JP2013539074A (ja) | 2010-09-24 | 2013-10-17 | カール・ツァイス・マイクロスコピー・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | ３ｄ局在顕微鏡法並びに４ｄ局在顕微鏡法及び追跡方法並びに追跡システム |
| WO2012058638A2 (en) | 2010-10-29 | 2012-05-03 | President And Fellows Of Harvard College | Nucleic acid nanostructure barcode probes |
| DE102010044013A1 (de) | 2010-11-16 | 2012-05-16 | Carl Zeiss Microimaging Gmbh | Tiefenauflösungsgesteigerte Mikroskopie |
| FR2978254B1 (fr) | 2011-07-21 | 2014-01-24 | Imagine Optic | Methode et dispositif optique pour la localisation d'une particule en super resolution |
| WO2013015734A1 (en) * | 2011-07-24 | 2013-01-31 | Applied Presicion, Inc. | Fluorescence microscopes with polychroic mirror changers |
| WO2013016356A1 (en) * | 2011-07-25 | 2013-01-31 | Mad City Labs, Inc. | Active-feedback positional drift correction in a microscope image using a fiduciary element held on a nanopositioning stage |
| DE102011053232B4 (de) | 2011-09-02 | 2020-08-06 | Leica Microsystems Cms Gmbh | Mikroskopische Einrichtung und mikroskopisches Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten |
| US10001622B2 (en) * | 2011-10-25 | 2018-06-19 | Sanford Burnham Medical Research Institute | Multifunction autofocus system and method for automated microscopy |
| DE102011055294B4 (de) | 2011-11-11 | 2013-11-07 | Leica Microsystems Cms Gmbh | Mikroskopische Einrichtung und Verfahren zur dreidimensionalen Lokalisierung von punktförmigen Objekten in einer Probe |
| US9291562B2 (en) * | 2011-11-15 | 2016-03-22 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | Method and apparatus for tracking a particle, particularly a single molecule, in a sample |
| US9693034B2 (en) | 2011-12-13 | 2017-06-27 | The Board Of Trustees Of The Leland Stanford Junior University | Apparatus and method for localizing objects for distance and/or in three dimensions using a spiral point spread function |
| WO2013090360A2 (en) | 2011-12-15 | 2013-06-20 | President And Fellows Of Harvard College | High resolution dual-objective microscopy |
| JP2013152429A (ja) * | 2011-12-27 | 2013-08-08 | Canon Inc | 画像処理装置、画像処理方法、およびプログラム |
| CN102565795B (zh) * | 2011-12-30 | 2014-01-15 | 北京华航无线电测量研究所 | 一种圆柱阵面三维成像系统的多角度成像显示方法 |
| CN102520408B (zh) * | 2011-12-30 | 2013-07-03 | 北京华航无线电测量研究所 | 一种圆柱阵面三维成像系统的三维成像方法 |
| CN102565796B (zh) * | 2011-12-30 | 2013-09-18 | 北京华航无线电测量研究所 | 一种圆柱阵面三维成像系统的成像方法 |
| DE102012200344A1 (de) | 2012-01-11 | 2013-07-11 | Carl Zeiss Microscopy Gmbh | Mikroskopsystem und Verfahren für die 3-D hochauflösende Mikroskopie |
| EP2966492B1 (en) | 2012-05-02 | 2020-10-21 | Centre National De La Recherche Scientifique | Method and apparatus for single-particle localization using wavelet analysis |
| EP2864468B1 (en) | 2012-06-22 | 2020-08-05 | The Regents of the University of Colorado, a body corporate | Method and system for estimating a position and an angular orientation of a particle |
| US20140022373A1 (en) * | 2012-07-20 | 2014-01-23 | University Of Utah Research Foundation | Correlative drift correction |
| JP6131568B2 (ja) * | 2012-10-30 | 2017-05-24 | 株式会社ニコン | 顕微鏡装置及び画像形成方法 |
| US9717402B2 (en) * | 2012-11-21 | 2017-08-01 | Align Technology, Inc. | Confocal imaging using astigmatism |
| US9063434B2 (en) | 2013-01-11 | 2015-06-23 | University Of Utah Research Foundation | Sub-diffraction-limited patterning and imaging via multi-step photoswitching |
| JP6349091B2 (ja) * | 2013-01-18 | 2018-06-27 | 国立大学法人 東京大学 | 超解像蛍光イメージング用プローブ |
| CN105307687A (zh) | 2013-03-15 | 2016-02-03 | 索隆-基特林癌症研究协会 | 多模态的基于二氧化硅的纳米粒子 |
| US9858464B2 (en) | 2013-03-15 | 2018-01-02 | The Regents Of The University Of Colorado, A Body Corporate | 3-D localization and imaging of dense arrays of particles |
| DE102013102988A1 (de) | 2013-03-22 | 2014-09-25 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| US9435993B2 (en) | 2013-03-24 | 2016-09-06 | Bruker Nano, Inc. | Three dimensional microscopy imaging |
| DE102013208926A1 (de) * | 2013-05-14 | 2014-11-20 | Carl Zeiss Microscopy Gmbh | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| DE102013208927A1 (de) * | 2013-05-14 | 2014-11-20 | Carl Zeiss Microscopy Gmbh | Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie |
| DE102013106895B4 (de) * | 2013-07-01 | 2015-09-17 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| WO2015031395A1 (en) | 2013-08-26 | 2015-03-05 | The Regents Of The University Of Colorado | Imaging through scattering media with high signal to noise ratio and resolution |
| DE102013015933A1 (de) | 2013-09-19 | 2015-03-19 | Carl Zeiss Microscopy Gmbh | Hochauflösende Scanning-Mikroskopie |
| WO2015046440A1 (ja) * | 2013-09-27 | 2015-04-02 | 株式会社ニコン | 解析装置、顕微鏡装置、解析方法、及びプログラム |
| CN104677865B (zh) * | 2013-12-02 | 2018-03-20 | 大连光耀辉科技有限公司 | 一种显微分析装置 |
| JP6284372B2 (ja) * | 2014-01-21 | 2018-02-28 | オリンパス株式会社 | 走査型レーザ顕微鏡および超解像画像生成方法 |
| US8977024B1 (en) * | 2014-01-31 | 2015-03-10 | Afraxis, Inc. | Distributed anatomical image analysis |
| JP6757255B2 (ja) | 2014-03-11 | 2020-09-16 | プレジデント アンド フェローズ オブ ハーバード カレッジ | プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング |
| US9810893B2 (en) | 2014-03-27 | 2017-11-07 | The Board Of Trustees Of The Leland Stanford Junior University | Phase mask imaging with characterization of rotational mobility |
| US20150302884A1 (en) * | 2014-04-18 | 2015-10-22 | Battelle Memorial Institute | Apparatus and method of multi-bit and gray-scale high density data storage |
| US10111963B2 (en) | 2014-05-29 | 2018-10-30 | Memorial Sloan Kettering Cancer Center | Nanoparticle drug conjugates |
| ES3014093T3 (en) | 2014-07-30 | 2025-04-16 | Harvard College | Method for determining nucleic acids |
| US20160069903A1 (en) | 2014-09-10 | 2016-03-10 | Fundació Institute De Ciències Foròniques | Method for detecting cells |
| EP3215806A4 (en) | 2014-11-05 | 2018-06-06 | The Regents Of The University Of Colorado | 3d imaging, ranging, and/or tracking using active illumination and point spread function engineering |
| EP3237890A4 (en) * | 2014-12-16 | 2018-11-07 | President and Fellows of Harvard College | Triggered assembly of metafluorophores |
| US9921161B1 (en) * | 2015-01-08 | 2018-03-20 | Daniel Feldkhun | Structured light active localization microscopy |
| JP6628969B2 (ja) * | 2015-03-06 | 2020-01-15 | シスメックス株式会社 | 細胞分析装置、細胞分析装置の制御方法およびプログラム |
| EP3276335B1 (en) * | 2015-03-27 | 2021-06-09 | Nikon Corporation | Microscope device, viewing method, and control program |
| DE102015105018A1 (de) * | 2015-03-31 | 2016-10-06 | Laser-Laboratorium Göttingen e.V. | Verfahren und Rasterfluoreszenzlichtmikroskop zum mehrdimensional hochauflösenden Abbilden einer Struktur oder eines Wegs eines Partikels in einer Probe |
| US10341640B2 (en) | 2015-04-10 | 2019-07-02 | The Board Of Trustees Of The Leland Stanford Junior University | Multi-wavelength phase mask |
| CA2991920C (en) * | 2015-04-23 | 2024-05-14 | The University Of British Columbia | Multifocal method and apparatus for stabilization of optical systems |
| US10736972B2 (en) | 2015-05-29 | 2020-08-11 | Memorial Sloan Kettering Cancer Center | Methods of treatment using ultrasmall nanoparticles to induce cell death of nutrient-deprived cancer cells via ferroptosis |
| EP3385769A4 (en) * | 2015-11-27 | 2019-10-30 | Nikon Corporation | MICROSCOPE DEVICE |
| KR101766064B1 (ko) * | 2015-11-30 | 2017-08-08 | 연세대학교 산학협력단 | 내부 전반사 형광 현미경 |
| US10783697B2 (en) | 2016-02-26 | 2020-09-22 | Yale University | Systems, methods, and computer-readable media for ultra-high resolution 3D imaging of whole cells |
| WO2017216603A1 (en) * | 2016-06-14 | 2017-12-21 | Evana Technologies, Uab | Laser processing method and a system for wafer dicing or cutting by use of a multi-segment focusing lens |
| JP6423841B2 (ja) * | 2016-10-11 | 2018-11-14 | 浜松ホトニクス株式会社 | 試料観察装置及び試料観察方法 |
| EP4386761A3 (en) | 2016-11-08 | 2024-09-04 | President and Fellows of Harvard College | Matrix imprinting and clearing |
| EP4339956A3 (en) | 2016-11-08 | 2024-06-19 | President and Fellows of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| DE102017211031A1 (de) | 2016-11-21 | 2018-05-24 | Carl Zeiss Microscopy Gmbh | Verfahren und Mikroskop zum Ermitteln einer Fluoreszenzintensität |
| WO2018102147A1 (en) * | 2016-11-29 | 2018-06-07 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | System and method for estimating and compensating for sample drift during data acquisition in fluorescence microscopy |
| AU2017370751B2 (en) | 2016-12-09 | 2023-11-09 | Ultivue, Inc. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
| CN106772975B (zh) * | 2017-01-19 | 2019-08-13 | 宁波纳美致生物科技有限公司 | 针对细胞或组织不同深度超分辨率显微成像的照明系统 |
| EP3634500B1 (en) | 2017-05-25 | 2023-10-25 | Memorial Sloan-Kettering Cancer Center | Ultrasmall nanoparticles labeled with zirconium-89 and methods thereof |
| US11788123B2 (en) | 2017-05-26 | 2023-10-17 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| DE102017111578A1 (de) | 2017-05-29 | 2018-11-29 | Wladimir Schaufler | Verfahren zur Unterscheidung einzelner fluoreszierender Markierungsstoffmoleküle in der SPDM-Lokalisationsmikroskopie durch deren zeitliches Langzeit-Emissionsverhalten über 10ms |
| EP3418391B1 (en) | 2017-06-23 | 2022-09-28 | Fundació Institut de Ciències Fotòniques | Method for quantifying protein copy-number |
| WO2018237253A1 (en) * | 2017-06-23 | 2018-12-27 | Memorial Sloan Kettering Cancer Center | Method of imaging in vivo tissues using nanoparticles comprising a reference dye and a sensor dye. |
| JP2020533563A (ja) * | 2017-08-30 | 2020-11-19 | バイオアキシアル エスエーエス | 特異分布と深層学習とに基づく超解像計測法 |
| WO2019135069A1 (en) * | 2018-01-02 | 2019-07-11 | King's College London | Method and system for localisation microscopy |
| CN113366116A (zh) | 2018-12-13 | 2021-09-07 | 哈佛学院院长及董事 | 用于merfish和其他应用的扩增方法和系统 |
| CN111323397B (zh) * | 2018-12-14 | 2023-03-28 | 深圳华大生命科学研究院 | 光学成像系统、成像检测系统与方法及基因测序方法 |
| WO2020186029A1 (en) | 2019-03-14 | 2020-09-17 | Applied Materials, Inc. | Identifying fiducial markers in microscope images |
| US11255785B2 (en) * | 2019-03-14 | 2022-02-22 | Applied Materials, Inc. | Identifying fiducial markers in fluorescence microscope images |
| DE102019108696B3 (de) | 2019-04-03 | 2020-08-27 | Abberior Instruments Gmbh | Verfahren zum Erfassen von Verlagerungen einer Probe gegenüber einem Objektiv |
| EP4062154A1 (en) * | 2019-11-20 | 2022-09-28 | President and Fellows of Harvard College | Methods for multi-focal imaging for molecular profiling |
| IL273118B (en) | 2020-03-05 | 2022-03-01 | Allen Richter | Self-adaptive electromagnetic radiation guide |
| KR102654655B1 (ko) * | 2020-10-20 | 2024-04-05 | 한양대학교 산학협력단 | 초고해상도 형광 현미경 이미징 방법 및 초고해상도 형광 현미경 이미징 장치 |
| KR102619825B1 (ko) * | 2020-10-20 | 2024-01-03 | 한양대학교 산학협력단 | 초고해상도 형광 현미경용 이미징 버퍼 용액 및 이를 이용한 초고해상도 형광 현미경 이미징 방법 |
| JP7563479B2 (ja) * | 2020-11-16 | 2024-10-08 | 株式会社ニコン | 顕微鏡およびプログラム |
| CN119693238A (zh) * | 2025-02-27 | 2025-03-25 | 深圳市真迈生物科技有限公司 | 提升图像分辨率的方法、系统及装置 |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1252408A (en) | 1916-09-25 | 1918-01-08 | Willard Daubenberg | Attachment for submarines. |
| US4549807A (en) * | 1983-10-07 | 1985-10-29 | At&T Bell Laboratories | Process for measuring fluorescence |
| US5736410A (en) * | 1992-09-14 | 1998-04-07 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| US6008373A (en) * | 1995-06-07 | 1999-12-28 | Carnegie Mellon University | Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer |
| BR9908767B1 (pt) * | 1998-03-16 | 2011-09-06 | sistema de formação de imagem confocal, sistema de focalização para uso com um substrato, e, processos de examinar um objeto, e de focalização para uso com um substrato. | |
| AU6842401A (en) | 2000-06-14 | 2001-12-24 | Clontech Lab Inc | Fluorescent timer proteins and methods for their use |
| JP2002062261A (ja) | 2000-08-21 | 2002-02-28 | Olympus Optical Co Ltd | 光学装置および顕微鏡 |
| US6844150B2 (en) * | 2000-08-24 | 2005-01-18 | The Regents Of The University Of California | Ultrahigh resolution multicolor colocalization of single fluorescent probes |
| US7309568B2 (en) * | 2001-09-26 | 2007-12-18 | Hitachi, Ltd. | Method in inspecting DNA and apparatus therefor |
| JP5414147B2 (ja) | 2003-04-13 | 2014-02-12 | マックス−プランク−ゲゼルシヤフト・ツーア・フェルデルング・デア・ヴィッセンシャフテン・アインゲトラーゲナー・フェライン | 立体的高解像度結像 |
| US7430045B2 (en) * | 2003-04-13 | 2008-09-30 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | High spatial resolution imaging |
| US7116590B2 (en) * | 2004-08-23 | 2006-10-03 | Micron Technology, Inc. | Memory address repair without enable fuses |
| US7054051B1 (en) | 2004-11-26 | 2006-05-30 | Alces Technology, Inc. | Differential interferometric light modulator and image display device |
| CN1779436A (zh) * | 2004-11-26 | 2006-05-31 | 上海爱建纳米科技发展有限公司 | 用于原子力显微镜的同轴照明显微镜光学系统 |
| RU2305270C2 (ru) | 2005-05-18 | 2007-08-27 | Андрей Алексеевич Климов | Способ флуоресцентной наноскопии (варианты) |
| EP3203235A1 (en) * | 2005-05-23 | 2017-08-09 | Harald F. Hess | Optical microscopy with phototransformable optical labels |
| JP4779518B2 (ja) | 2005-09-08 | 2011-09-28 | パナソニック株式会社 | 蛍光読取装置および微生物計数装置 |
| JP4572162B2 (ja) | 2005-11-22 | 2010-10-27 | 株式会社ジーオングストローム | 顕微鏡装置 |
| US7564544B2 (en) * | 2006-03-22 | 2009-07-21 | 3i Systems Corporation | Method and system for inspecting surfaces with improved light efficiency |
| DE102006021317B3 (de) | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US7838302B2 (en) | 2006-08-07 | 2010-11-23 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| US7776613B2 (en) | 2006-08-07 | 2010-08-17 | President And Fellows Of Harvard College | Sub-diffraction image resolution and other imaging techniques |
| JP4609418B2 (ja) * | 2006-11-15 | 2011-01-12 | トヨタ自動車株式会社 | シフト切換機構の制御装置および制御方法 |
| CN100529737C (zh) * | 2006-11-28 | 2009-08-19 | 南开大学 | 超短脉冲激光诱导的多光子电离非线性显微成像方法和系统及其应用 |
| US7924432B2 (en) * | 2006-12-21 | 2011-04-12 | Howard Hughes Medical Institute | Three-dimensional interferometric microscopy |
| US20080241843A1 (en) * | 2006-12-21 | 2008-10-02 | Stanford University | Single-cell analysis systems, methods of counting molecules in a single-cell, cylindrical fluorescence detection systems |
| US8217992B2 (en) * | 2007-01-11 | 2012-07-10 | The Jackson Laboratory | Microscopic imaging techniques |
| EP2008449B1 (en) | 2007-01-30 | 2011-10-12 | F. Poszat HU, L.L.C. | Image transfer apparatus |
| EP2012173A3 (en) | 2007-07-03 | 2009-12-09 | JDS Uniphase Corporation | Non-etched flat polarization-selective diffractive optical elements |
| CN101918816B (zh) | 2007-12-21 | 2015-12-02 | 哈佛大学 | 三维中的亚衍射极限图像分辨率 |
| US7772569B2 (en) * | 2008-04-01 | 2010-08-10 | The Jackson Laboratory | 3D biplane microscopy |
| US8248609B2 (en) * | 2008-11-04 | 2012-08-21 | The Johns Hopkins University | Cylindrical illumination confocal spectroscopy system |
| DE102008054438A1 (de) | 2008-12-09 | 2010-06-24 | Seereal Technologies S.A. | Optisches Bauteil zum Ablenken von das optische Bauteil durchlaufende Lichtstrahlen |
| WO2010143601A1 (ja) | 2009-06-11 | 2010-12-16 | 国立大学法人筑波大学 | ２ビーム型光コヒーレンストモグラフィー装置 |
| US8101129B2 (en) | 2009-10-13 | 2012-01-24 | Chuang Shu-Yuan | Nano filter structure for breathing and manufacturing method thereof |
| US9492322B2 (en) | 2009-11-16 | 2016-11-15 | Alcon Lensx, Inc. | Imaging surgical target tissue by nonlinear scanning |
| JP2013539074A (ja) | 2010-09-24 | 2013-10-17 | カール・ツァイス・マイクロスコピー・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング | ３ｄ局在顕微鏡法並びに４ｄ局在顕微鏡法及び追跡方法並びに追跡システム |
| US20140333750A1 (en) | 2011-12-15 | 2014-11-13 | President And Fellows Of Harvard College | High resolution dual-objective microscopy |
| ES2898616T3 (es) | 2012-04-03 | 2022-03-08 | Univ Court Univ St Andrews | Imágenes de alta resolución de volúmenes extendidos |
| WO2015117115A1 (en) | 2014-02-03 | 2015-08-06 | President And Fellows Of Harvard College | Three-dimensional super-resolution fluorescence imaging using airy beams and other techniques |
-
2008
- 2008-12-19 CN CN200880121492.2A patent/CN101918816B/zh active Active
- 2008-12-19 CN CN201510702700.XA patent/CN105403545B/zh active Active
- 2008-12-19 JP JP2010539499A patent/JP5526036B2/ja active Active
- 2008-12-19 US US12/746,784 patent/US8564792B2/en active Active
- 2008-12-19 WO PCT/US2008/013915 patent/WO2009085218A1/en not_active Ceased
- 2008-12-19 EP EP08866669A patent/EP2232244A1/en not_active Withdrawn
- 2008-12-19 EP EP22172052.7A patent/EP4060327A1/en not_active Withdrawn
-
2013
- 2013-05-21 US US13/899,215 patent/US9077975B2/en active Active
- 2013-09-09 US US14/022,168 patent/US9137516B2/en active Active
-
2014
- 2014-01-09 JP JP2014002163A patent/JP2014066734A/ja not_active Withdrawn
- 2014-07-23 JP JP2014149455A patent/JP2014199260A/ja not_active Withdrawn
-
2015
- 2015-08-07 US US14/821,569 patent/US9712805B2/en active Active
-
2016
- 2016-11-17 JP JP2016223789A patent/JP6452663B2/ja active Active
-
2017
- 2017-06-15 US US15/623,658 patent/US10412366B2/en active Active
-
2018
- 2018-12-11 JP JP2018231326A patent/JP2019070820A/ja active Pending
-
2019
- 2019-07-31 US US16/527,739 patent/US20200099916A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6452663B2 (ja) | 三次元の回折限界未満の画像解像技術 | |
| JP2014066734A5 (enExample) | ||
| JP6661154B2 (ja) | 回折限界以下の画像解像技術および他の画像化技術 | |
| US11988604B2 (en) | Optical microscopy with phototransformable optical labels | |
| US20140333750A1 (en) | High resolution dual-objective microscopy | |
| WO2013090360A2 (en) | High resolution dual-objective microscopy | |
| WO2015117115A1 (en) | Three-dimensional super-resolution fluorescence imaging using airy beams and other techniques |