CN105393741A - Breeding method for further optimizing wild improved variety of dendrobium huoshanense - Google Patents

Breeding method for further optimizing wild improved variety of dendrobium huoshanense Download PDF

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Publication number
CN105393741A
CN105393741A CN201510695786.8A CN201510695786A CN105393741A CN 105393741 A CN105393741 A CN 105393741A CN 201510695786 A CN201510695786 A CN 201510695786A CN 105393741 A CN105393741 A CN 105393741A
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pollination
dendrobium
wild
plant
carry out
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CN201510695786.8A
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何斌
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Anhui Huoshan County Yaowanggu Forest & Agricultural Science & Technology Co Ltd
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Anhui Huoshan County Yaowanggu Forest & Agricultural Science & Technology Co Ltd
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Priority to CN201510695786.8A priority Critical patent/CN105393741A/en
Publication of CN105393741A publication Critical patent/CN105393741A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a breeding method for further optimizing a wild improved variety of dendrobium huoshanense. The method comprises steps: (1) building a base; (2) planting plants; (3) managing after planting; (4) performing artificial pollination; (5) performing management after pollination; (6) and harvesting fruits. In the method, artificial pollination is performed by mutual pollination between each two clusters of plants, so as to maintain good hereditary stability, and improve stress resistance and growing speed of the plants. Arranged umbrella-type protective screens further ensure activities of insects would not conduct pollens among different plants in the same cluster and among plants in different clusters or damage pollination. Applied organic fertilizer is prepared according to natural growth laws of dendrobium, and the organic fertilizer is rich in nutrient, and is natural organic matter, and would not cause any damage to the dendrobium or cause drug residue, thereby ensuring quality of the dendrobium and security. Germination rate of seeds bred by the method is high, seedlings are in order and stable in property, and are good in stress resistance, and vigorous in growth vigor.

Description

The mating system that the wild breeding of a kind of Dendrobidium huoshanness is optimized further
Technical field
The invention belongs to field of planting, be specifically related to the mating system that the wild breeding of a kind of Dendrobidium huoshanness is optimized further.
Background technology
Dendrobidium huoshanness (formal name used at school: DendrobiumhuoshanenseC.Z.TangetS.J.Cheng) is commonly called as a meter dry measure used in former times, is the herbaceous plant of the orchid family Dendrobium.Stem is upright, meat, not branch, and tool 3-7 saves, pistac, is sometimes with lilac red spot, faint yellow after dry.Leaf keratin, 2-3 piece of alternate, in the top of stem, tiltedly goes out, ligule Long Circle.Raceme 1-3, send from the old stem top of the leaf that fallen, a tool 1-2 flower; Flower pistac, carries out; Petal ovum shape Long Circle, tip is blunt, tool 5 arteries and veins; The nearly rhombus of lip, long and wide about equal.China national geography famous special product.Main product is in Huoshan County of Anhui Province of Da Bie Mountain area, and mostly growth is between the overhanging cliff cliffstone gap that cloud and mist curls up and on old trees that reach into the skies, to be born in the woods of mountain region on trunk and on the rock of mountain valley.Dendrobidium huoshanness can increase substantially the main matter that in human body, SOD(delays senility) level, to often staying up late, require mental skill, tobacco and wine are excessive, and the empty weak crowd of body, it is very suitable often to drink.Dendrobidium huoshanness has function of improving eyesight, also YIN and YANG balance regulating, strengthening yang and invigorating kidney, beauty treatment can stay and holds, thus reach effect of health-care.
Mostly stem of noble dendrobium natural breed is self-pollination, and having a small amount of is cross pollination, and pollen is invested in beggar room by the main gathering honey action by insect, completes pollination.Also have the benefit reported and adopt cross pollination at present, but the present inventor finds that in a large amount of experiments seedling still exists uneven, that performance difference is larger phenomenon, the demand optimizing provenance can not be met.
Summary of the invention
In order to solve the problem, the invention provides the mating system that the wild breeding of a kind of Dendrobidium huoshanness is optimized further, further can optimize original breeding, improve quality, expanding production.
The present invention realizes by following technical solution:
The mating system that the wild breeding of Dendrobidium huoshanness is optimized further, comprises the steps:
(1) construction of base: imitate on wild base at suitable Dendrobidium huoshanness growing environment and cultivation seedbed is set, seedbed is laid the thick culture medium of 8 ~ 12cm;
(2) plant field planting: the wild breeding of the Dendrobidium huoshanness that outward appearance is good, growing way is vigorous is colonizated on seedbed with the distance between rows and hills of 40 ~ 45cm × 35 ~ 40cm;
(3) manage after field planting: to pollination, execute twice fertilizer after field planting, fertilizer will cover fully, applied before at 10 in the morning at every turn;
(4) artificial pollination: the flowers are in blossom 2 days time carry out artificial pollination, carry out in the mode of pollination mutually between every two clumps, choose two clumps of dendrobium plant, carry out mark, be designated as A, B respectively, male column cap pollen toothpick is spent by the A clump stem of noble dendrobium to take off, and be positioned in the glass dish disinfected in advance, pollinated again in the style ovary of B clump dendrobium plant, in like manner, spent by the B clump stem of noble dendrobium male column cap pollen to pollinate in the same way in the style ovary of A clump dendrobium plant, all the other plant pollinate in this way between two, and carry out mark;
(5) pollination after management: the umbrella-shaped protective screening cover making wide 20 ~ 25cm, high 15 ~ 20cm, is put in above the dendrobium plant after pollination, every morning 8:00 ~ 9:00 carry out for time 1h Artificial Fog keep the skin wet, and every 30 ~ 40 days apply a fertilizer.
(6) fruit harvest: after fruit maturation, for subsequent use as planting fruit after plucking.
Further, step (3), fertilizer described in (5) are made up of the material of following weight portion: 15 ~ 20 parts of rapeseed dregs, 10 ~ 15 parts of silkworm excrements, 5 ~ 8 parts of humus, 10 ~ 13 parts of ash.
Further, step (4) the described artificial pollination time is selected in the 8:00 ~ 10:00 in fine day morning and directly carries out.
Further, step (5) described guard order number is 70 ~ 80 orders.
The present invention has following beneficial effect: the present invention adopts the mode of pollination mutually between every two group planting strains to carry out artificial pollination, maintain the genetic stability that offspring is good, turn improve its resistance and growth rate, the activity that the umbrella-shaped protective screening simultaneously arranged further ensures insect etc. does not carry out pollen conduction or infringement between same Cong Yi strain and different group planting strain, the organic manure applied forms according to the rule configuration of stem of noble dendrobium self-sow, not only nutritious, and be natural organic matter, any infringement and medicament residue can not be caused to the stem of noble dendrobium, ensure that its quality and safety.The germination rate that this method brings out seed is high, and seedling is neat, and proterties is stablized, and strong stress resistance, growing way is vigorous.
Embodiment
Embodiment 1
The mating system that the wild breeding of Dendrobidium huoshanness is optimized further, comprises the steps:
(1) construction of base: be suitable in imitative wild base Dendrobidium huoshanness growing environment base is arranged and cultivate seedbed, seedbed is laid the culture medium that 8cm is thick;
(2) plant field planting: the wild breeding of the Dendrobidium huoshanness that outward appearance is good, growing way is vigorous is colonizated on seedbed with the distance between rows and hills of 40cm × 35cm;
(3) manage after field planting: to pollination, execute twice fertilizer after field planting, fertilizer will cover fully, applied before at 10 in the morning at every turn;
(4) artificial pollination: the flowers are in blossom 2 days time carry out artificial pollination, carry out in the mode of pollination mutually between every two clumps, choose two clumps of dendrobium plant, carry out mark, be designated as A, B respectively, male column cap pollen toothpick is spent by the A clump stem of noble dendrobium to take off, and be positioned in the glass dish disinfected in advance, pollinated again in the style ovary of B clump dendrobium plant, in like manner, spent by the B clump stem of noble dendrobium male column cap pollen to pollinate in the same way in the style ovary of A clump dendrobium plant, all the other plant pollinate in this way between two, and carry out mark;
(5) pollination after management: the umbrella-shaped protective screening cover making wide 20cm, high 16cm, is put in above the dendrobium plant after pollination, every morning 8:00 ~ 9:00 carry out for time 1h Artificial Fog keep the skin wet, and every 35 days apply a fertilizer.
(6) fruit harvest: after fruit maturation, for subsequent use as planting fruit after plucking.
Further, step (3), fertilizer described in (5) are made up of the material of following weight portion: 15 parts of rapeseed dregs, 12 parts of silkworm excrements, 6 parts of humus, 10 parts of ash.
Further, step (4) the described artificial pollination time is selected in the 8:00 ~ 10:00 in fine day morning and directly carries out.
Further, step (5) described guard order number is 70 orders.
The percentage of fertile fruit of the present embodiment can up to more than 97%, completely filled fruit, and percentage of seedgermination is more than 45%, and it is vigorous that seedling growth is educated by institute, and seedling is neat and strengthen, and anti-adversity, growth rate etc. comparatively male parent female parent promote all to some extent, optimized varieties success.
Embodiment 2
The mating system that the wild breeding of Dendrobidium huoshanness is optimized further, comprises the steps:
(1) construction of base: imitate on wild base at suitable Dendrobidium huoshanness growing environment and cultivation seedbed is set, seedbed is laid the culture medium that 12cm is thick;
(2) plant field planting: the wild breeding of the Dendrobidium huoshanness that outward appearance is good, growing way is vigorous is colonizated on seedbed with the distance between rows and hills of 45cm × 38cm;
(3) manage after field planting: to pollination, execute twice fertilizer after field planting, fertilizer will cover fully, applied before at 10 in the morning at every turn;
(4) artificial pollination: the flowers are in blossom 2 days time carry out artificial pollination, carry out in the mode of pollination mutually between every two clumps, choose two clumps of dendrobium plant, carry out mark, be designated as A, B respectively, male column cap pollen toothpick is spent by the A clump stem of noble dendrobium to take off, and be positioned in the glass dish disinfected in advance, pollinated again in the style ovary of B clump dendrobium plant, in like manner, spent by the B clump stem of noble dendrobium male column cap pollen to pollinate in the same way in the style ovary of A clump dendrobium plant, all the other plant pollinate in this way between two, and carry out mark;
(5) pollination after management: the umbrella-shaped protective screening cover making wide 25cm, high 20cm, is put in above the dendrobium plant after pollination, every morning 8:00 ~ 9:00 carry out for time 1h Artificial Fog keep the skin wet, and every 30 days apply a fertilizer.
(6) fruit harvest: after fruit maturation, for subsequent use as planting fruit after plucking.
Further, step (3), fertilizer described in (5) are made up of the material of following weight portion: 20 parts of rapeseed dregs, 12 parts of silkworm excrements, 8 humus, 13 parts of ash.
Further, step (4) the described artificial pollination time is selected in the 8:00 ~ 10:00 in fine day morning and directly carries out.
Further, step (5) described guard order number is 80 orders.
The percentage of fertile fruit of the present embodiment can up to more than 98%, completely filled fruit, and percentage of seedgermination is more than 48%, and it is vigorous that seedling growth is educated by institute, and seedling is shape together, and anti-adversity, growth rate etc. comparatively male parent female parent promote all to some extent, optimized varieties success.

Claims (4)

1. the mating system optimized further of the wild breeding of Dendrobidium huoshanness, is characterized in that, comprise the steps:
(1) construction of base: select imitative wild base to be suitable for Dendrobidium huoshanness environmental foundations being arranged cultivation seedbed, seedbed is laid the thick culture medium of 8 ~ 12cm;
(2) plant field planting: the wild breeding of the Dendrobidium huoshanness that outward appearance is good, growing way is vigorous is colonizated on seedbed with the distance between rows and hills of 40 ~ 45cm × 35 ~ 40cm;
(3) manage after field planting: to pollination, execute twice fertilizer after field planting, fertilizer will cover fully, applied before at 10 in the morning at every turn;
(4) artificial pollination: the flowers are in blossom 2 days time carry out artificial pollination, carry out in the mode of pollination mutually between every two clumps, choose two clumps of dendrobium plant, carry out mark, be designated as A, B respectively, male column cap pollen toothpick is spent by the A clump stem of noble dendrobium to take off, and be positioned in the glass dish disinfected in advance, pollinated again in the style ovary of B clump dendrobium plant, in like manner, spent by the B clump stem of noble dendrobium male column cap pollen to pollinate in the same way in the style ovary of A clump dendrobium plant, all the other plant pollinate in this way between two, and carry out mark;
(5) pollination after management: the umbrella-shaped protective screening cover making wide 20 ~ 25cm, high 15 ~ 20cm, is put in above the dendrobium plant after pollination, every morning 8:00 ~ 9:00 carry out for time 1h Artificial Fog keep the skin wet, and every 30 ~ 40 days apply a fertilizer;
(6) fruit harvest: after fruit maturation, for subsequent use as planting fruit after plucking.
2. the mating system optimized further of the wild breeding of a kind of Dendrobidium huoshanness according to claim 1, it is characterized in that, step (3), fertilizer described in (5) are made up of the material of following weight portion: 15 ~ 20 parts of rapeseed dregs, 10 ~ 15 parts of silkworm excrements, 5 ~ 8 parts of humus, 10 ~ 13 parts of ash.
3. the mating system optimized further of the wild breeding of a kind of Dendrobidium huoshanness according to claim 1, is characterized in that, 8:00 ~ 10:00 that step (4) the described artificial pollination time is selected in fine day morning directly carries out.
4. the mating system optimized further of the wild breeding of a kind of Dendrobidium huoshanness according to claim 1, it is characterized in that, step (5) described guard order number is 70 ~ 80 orders.
CN201510695786.8A 2015-10-26 2015-10-26 Breeding method for further optimizing wild improved variety of dendrobium huoshanense Pending CN105393741A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114651677A (en) * 2022-04-06 2022-06-24 宁夏大学 Method for optimizing artificial breeding conditions of wild sweet clover-shaped astragalus seeds in arid region

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005192480A (en) * 2004-01-07 2005-07-21 Masakatsu Yato Dendrobium
CN101536668A (en) * 2009-01-14 2009-09-23 大连普瑞康生物技术有限公司 Hybridization pollination method of dendrobium officinale
CN102657078A (en) * 2012-05-25 2012-09-12 苏州神元生物科技有限公司 Method of artificial pollination for Dendrobium candidum
CN103109661A (en) * 2013-01-31 2013-05-22 安徽霍山县药王谷林农科技有限公司 Method of imitating wild cultivation in dendrobe forest
CN104025987A (en) * 2014-06-24 2014-09-10 韶关车八岭农业科技有限公司 Dendrobium officinale planting method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005192480A (en) * 2004-01-07 2005-07-21 Masakatsu Yato Dendrobium
CN101536668A (en) * 2009-01-14 2009-09-23 大连普瑞康生物技术有限公司 Hybridization pollination method of dendrobium officinale
CN102657078A (en) * 2012-05-25 2012-09-12 苏州神元生物科技有限公司 Method of artificial pollination for Dendrobium candidum
CN103109661A (en) * 2013-01-31 2013-05-22 安徽霍山县药王谷林农科技有限公司 Method of imitating wild cultivation in dendrobe forest
CN104025987A (en) * 2014-06-24 2014-09-10 韶关车八岭农业科技有限公司 Dendrobium officinale planting method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114651677A (en) * 2022-04-06 2022-06-24 宁夏大学 Method for optimizing artificial breeding conditions of wild sweet clover-shaped astragalus seeds in arid region

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