CN105378486B - 生产omega-羟基化的脂肪酸衍生物的方法 - Google Patents

生产omega-羟基化的脂肪酸衍生物的方法 Download PDF

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CN105378486B
CN105378486B CN201480033702.8A CN201480033702A CN105378486B CN 105378486 B CN105378486 B CN 105378486B CN 201480033702 A CN201480033702 A CN 201480033702A CN 105378486 B CN105378486 B CN 105378486B
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fatty acid
omega
escherichia coli
hydroxy fatty
recombinant escherichia
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CN105378486A (zh
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A·W·席尔默
H·王
S·B·德尔卡黛莉
胡志浩
L·G·霍姆
B·朱
C·常
E·E·波波瓦
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Genomatica Inc
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Genomatica Inc
REG Life Sciences LLC
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CN201480033702.8A 2013-06-14 2014-06-16 生产omega-羟基化的脂肪酸衍生物的方法 Active CN105378486B (zh)

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CN202410503321.7A CN118460487A (zh) 2013-06-14 2014-06-16 生产omega-羟基化的脂肪酸衍生物的方法
CN202410501856.0A CN118374462A (zh) 2013-06-14 2014-06-16 生产omega-羟基化的脂肪酸衍生物的方法

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US201361835464P 2013-06-14 2013-06-14
US61/835,464 2013-06-14
PCT/US2014/042594 WO2014201474A1 (en) 2013-06-14 2014-06-16 Methods of producing omega-hydroxylated fatty acid derivatives

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US (3) US10711288B2 (https=)
EP (2) EP3745133A1 (https=)
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AU (2) AU2014277874B2 (https=)
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Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220053681A (ko) 2013-06-14 2022-04-29 게노마티카 인코포레이티드 오메가-수산화 지방산 유도체를 생성하는 방법들
US11008597B2 (en) 2014-04-10 2021-05-18 Genomatica, Inc. Chemo-enzymatic process
WO2015176020A1 (en) 2014-05-16 2015-11-19 Provivi, Inc. Synthesis of olefinic alcohols via enzymatic terminal hydroxylation
CA2951925A1 (en) * 2014-06-16 2015-12-23 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptides with improved properties
KR20170029625A (ko) 2014-07-18 2017-03-15 알이지 라이프 사이언시스, 엘엘씨 지방 디올의 미생물 생산
US11319562B2 (en) 2015-04-15 2022-05-03 Ramon Gonzalez Modified fatty acid biosynthesis with ACP-dependent thiolases
WO2016168247A1 (en) * 2015-04-15 2016-10-20 William Marsh Rice University Modified fatty acid biosynthesis with acp-dependent thiolases
KR101903551B1 (ko) * 2015-10-27 2018-10-04 한국생명공학연구원 중쇄 디올의 생산 방법
KR101903553B1 (ko) * 2015-10-27 2018-10-04 한국생명공학연구원 중쇄 아미노카르복시산의 생산 방법
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
CN107201356A (zh) * 2016-03-17 2017-09-26 中国科学院青岛生物能源与过程研究所 支撑p450脂肪酸脱羧酶活性的还原伴侣蛋白组合及其应用
CN111094558A (zh) 2017-04-03 2020-05-01 基因组股份公司 对产生中链脂肪酸衍生物具有改善的活性的硫酯酶变体
KR102170282B1 (ko) * 2017-12-15 2020-10-29 건국대학교 산학협력단 α,ω-디올류 생산하는 재조합 미생물 및 α,ω-디올류의 생산방법
TWI701040B (zh) * 2018-03-19 2020-08-11 國立臺灣大學 蛋白質的用途及包含該蛋白質之全細胞催化系統及其應用
EP3792351A4 (en) 2018-04-16 2022-01-19 Korea Research Institute of Bioscience and Biotechnology Method for producing multi-hydroxy derivatives of polyunsaturated fatty acids
EP3824071A4 (en) * 2018-07-17 2022-04-20 Conagen Inc. BIOSYNTHETIC MANUFACTURE OF GAMMA LACTONES
KR102117828B1 (ko) 2018-07-19 2020-06-02 서울대학교산학협력단 레귤론 변이를 통한 지방산 및 지방산 유도체 생산에서 불포화도 감소
US12098168B2 (en) 2018-08-31 2024-09-24 Genomatica, Inc. XylR mutant for improved xylose utilization or improved co-utilization of glucose and xylose preliminary
CN111218485B (zh) * 2018-11-26 2023-01-24 中国科学院大连化学物理研究所 一种生产ω-1-羟基脂肪酸的方法
CN111218430B (zh) * 2018-11-26 2022-06-14 中国科学院大连化学物理研究所 一种nadh类似物依赖型的细胞色素p450还原酶及其应用
CN111592996A (zh) * 2019-12-09 2020-08-28 福建省水产研究所(福建水产病害防治中心) 一种芽孢杆菌cqn-2高密度发酵培养方法
US20240229086A1 (en) * 2021-03-29 2024-07-11 Genomatica, Inc. Production Of Non-Native Monounsaturated Fatty Acids In Bacteria
JP2023005283A (ja) * 2021-06-28 2023-01-18 出光興産株式会社 ペプチドタグ付加タンパク質を用いた代謝物生産
WO2023178211A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Microorganisms and methods for production of fatty acid derivatives with reduced levels of byproducts
CN114480513A (zh) * 2022-04-01 2022-05-13 北京林业大学 一种基因编辑酵母体外合成麝香酮的方法
CN115537369B (zh) * 2022-11-09 2024-03-01 云南农业大学 2-十二烯二酸促进蒙氏假单胞菌生长和定殖的应用
CN116064362B (zh) * 2022-12-15 2025-10-28 浙江大学 一种利用葡萄糖从头合成12-氨基月桂酸的基因工程菌及其构建和应用
CN116790529B (zh) * 2023-05-24 2025-01-17 天津大学 P450 bm3蛋白突变体及其应用
CN117343970B (zh) * 2023-10-24 2025-01-21 杭州佳嘉乐生物技术有限公司 一种提升植物油脂亲肤感的油脂发酵方法
CN120699859A (zh) * 2024-11-13 2025-09-26 中国科学院深圳先进技术研究院 重组菌及其构建方法和应用
CN120254135B (zh) * 2025-06-04 2025-08-29 中国农业科学院北京畜牧兽医研究所 一种高精度、抗干扰的内毒素分析方法及试剂盒

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1305083C (en) 1987-02-18 1992-07-14 Yoshiharu Kimura Microorganism belonging to genus rhodococcus, and a process for producing alkene derivative and unsaturated fatty acid
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
WO1999018224A1 (en) 1997-10-06 1999-04-15 The Centre National De Recherche Scientifique Plant fatty acid hydroxylase genes
RU2352549C2 (ru) 2002-04-29 2009-04-20 Дау Глобал Текнолоджиз Инк. Интегрированные химические способы промышленного применения растительных масел из семян
US20040242831A1 (en) 2003-05-30 2004-12-02 Dong Tian Enzyme catalyzed polyesters and polyol polymers
WO2006051729A1 (ja) 2004-11-10 2006-05-18 Marine Biotechnology Institute Co., Ltd. P450遺伝子の単離法
JP2009079057A (ja) 2005-04-22 2009-04-16 Mitsubishi Chemicals Corp ポリエステル及びその製造方法
EP2024491B1 (en) 2006-05-19 2014-11-19 LS9, Inc. Production of fatty acids and derivatives thereof
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) * 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
DK2121546T3 (en) 2006-10-13 2018-03-12 Elevance Renewable Sciences Process for preparing omega-dicarboxylic acid olefin derivative by metathesis
EP2076484B1 (en) 2006-10-13 2020-01-08 Elevance Renewable Sciences, Inc. Synthesis of terminal alkenes from internal alkenes via olefin metathesis
US8110093B2 (en) 2007-03-14 2012-02-07 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CA3178593A1 (en) 2007-03-28 2008-10-02 Genomatica, Inc. Enhanced production of fatty acid derivatives
US20080293060A1 (en) 2007-04-23 2008-11-27 Ls9, Inc. Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes
JP5912251B2 (ja) 2007-05-22 2016-06-01 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 炭化水素産生遺伝子およびその使用方法
JP5324129B2 (ja) * 2007-05-25 2013-10-23 神戸天然物化学株式会社 Cyp153による芳香族化合物の製造方法
US20100199548A1 (en) 2007-07-06 2010-08-12 Ls9, Inc. Systems and methods for the production of fatty esters
FR2921362B1 (fr) 2007-09-20 2012-09-21 Arkema France Procede de synthese d'acides gras omega-insatures
CA2692266C (en) 2007-09-27 2019-04-16 Ls9, Inc. Reduction of the toxic effect of impurities from raw materials by extractive fermentation
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
JP2011520455A (ja) 2008-05-16 2011-07-21 エルエス9・インコーポレイテッド 炭化水素を製造するための方法および組成物
WO2009142983A1 (en) 2008-05-23 2009-11-26 Alliant Techsystems Inc. Broadband patch antenna and antenna system
US8273694B2 (en) 2008-07-28 2012-09-25 Jeffrey A Brown Synthetic compositions obtained from algae
US20100071259A1 (en) 2008-08-18 2010-03-25 Ls9, Inc. Systems and methods for production of mixed fatty esters
CN102131752A (zh) 2008-08-28 2011-07-20 三井化学株式会社 烯烃制造方法
JP2012504963A (ja) 2008-10-07 2012-03-01 エルエス9・インコーポレイテッド 脂肪アルデヒドを生産するための方法および組成物
KR101700296B1 (ko) 2008-10-28 2017-01-26 알이지 라이프 사이언시스, 엘엘씨 지방족 알코올들을 생산하기 위한 방법들과 조성물들
US7989875B2 (en) 2008-11-24 2011-08-02 Nxp B.V. BiCMOS integration of multiple-times-programmable non-volatile memories
EP3564345A1 (en) 2008-12-23 2019-11-06 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
US8071799B2 (en) 2009-01-29 2011-12-06 Energy & Environmental Research Center Foundation Chain-selective synthesis of fuel components and chemical feedstocks
JP5353768B2 (ja) 2009-03-06 2013-11-27 国立大学法人東京工業大学 樹脂組成物
CA2758298A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
CN111808892A (zh) 2009-04-27 2020-10-23 基因组股份公司 脂肪酸酯的产生
BRPI1009980A2 (pt) 2009-05-01 2015-08-25 Univ California Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa
CN105969710A (zh) 2009-09-25 2016-09-28 Reg生命科学有限责任公司 脂肪酸衍生物的产生
US8237003B2 (en) 2009-11-09 2012-08-07 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8809563B2 (en) 2009-11-09 2014-08-19 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8361769B1 (en) 2009-11-16 2013-01-29 U.S. Department Of Energy Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
US20110124071A1 (en) 2009-11-17 2011-05-26 LS9, Inc Methods and compositions for producing hydrocarbons
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
US20110250663A1 (en) * 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
SG10201504187YA (en) 2010-05-28 2015-06-29 Solazyme Inc Tailored oils produced from recombinant heterotrophic microorganisms
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
JP2012097163A (ja) * 2010-10-29 2012-05-24 Dainippon Printing Co Ltd ポリエステル樹脂組成物
WO2012071439A1 (en) 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
MX344012B (es) 2011-02-02 2016-12-02 Terravia Holdings Inc Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes.
WO2013006733A2 (en) * 2011-07-06 2013-01-10 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
EP2639308A1 (de) * 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
KR20210118216A (ko) * 2012-10-15 2021-09-29 게노마티카 인코포레이티드 특정 길이의 지방 알콜 및 관련 화합물의 생산을 위한 미생물 및 방법
FR3003577B1 (fr) 2013-03-19 2016-05-06 Ferropem Inoculant a particules de surface
KR20220053681A (ko) 2013-06-14 2022-04-29 게노마티카 인코포레이티드 오메가-수산화 지방산 유도체를 생성하는 방법들
CA2951925A1 (en) 2014-06-16 2015-12-23 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptides with improved properties
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Bacterial CYP153A monooxygenases for the synthesis of omega-hydroxylated fatty acids;Sumire Honda et al.;《ChemComm》;20120326;第48卷;摘要部分 *

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