CN105362368B - A kind of herbal composite and its preparation method and application with acne-removal function - Google Patents
A kind of herbal composite and its preparation method and application with acne-removal function Download PDFInfo
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- CN105362368B CN105362368B CN201510329409.2A CN201510329409A CN105362368B CN 105362368 B CN105362368 B CN 105362368B CN 201510329409 A CN201510329409 A CN 201510329409A CN 105362368 B CN105362368 B CN 105362368B
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Abstract
The invention discloses a kind of herbal composites with acne-removal function, its raw material is grouped as by following group: 10~20 parts by weight of strophanthus divaricatus, 4~8 parts by weight of radix scutellariae, 6~10 parts by weight of 6~10 parts by weight of balsam pear, 4~8 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra, preparation method includes: that will extract to obtain concentrate with 20~30% alcohol refluxs after above-mentioned each component co-grinding, the concentrate is isolated and purified into obtain eluent through AB-8 macroporous absorbent resin, by the eluent through heating in vacuum concentration and vacuum freeze drying to get the herbal composite with acne-removal function.Herbal composite of the invention has the features such as using safe, significant curative effect, uneasy to recur to the treatment of facial acne vulgaris.It can avoid the side effect generated when prolonged application hormone and antibiotic, while also having certain positive effect on conditioning skin state, improvement Endocrine Levels.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, and in particular to a kind of herbal composite and its preparation with acne-removal function
Methods and applications.
Background technique
Acne (Acne) is common chronic pilosebaceous inflammations dermatoses, and clinical manifestation is blackhead, mound
Rash, warts, tumour, tubercle, and visible cicatrization.It is apt to occur in face, neck, trunk top etc..Disease incidence is higher, and 90% or more
Puberty men and women can have different degrees of skin lesion, and serious person can cause to disfeature, and 15% state of an illness is heavier and needs to treat.
The treatment of acne is always one of the heat subject of dermatology's research, and the cause of disease is complicated, belongs to multi-factor comprehensive and causes a disease,
The definite cause of disease is not yet completely understood.At present both at home and abroad to the treatment of acne vulgaris, mostly used according to the severity of acne lesions
Oral antibiotic, retinoid drug, antiandrogen drug etc..Though there is certain therapeutic effect, there is recurrence, liver kidney after being discontinued
The side effects such as damage and gastrointestinal reaction.
Summary of the invention
It is an object of the invention to overcome prior art defect, a kind of herbal composite with acne-removal function is provided.
Another object of the present invention is to provide the preparation methods of the above-mentioned herbal composite with acne-removal function.
A further object of the present invention is to provide the applications of said herbal medicine composition.
The specific technical solution of the present invention is as follows:
A kind of herbal composite with acne-removal function, raw material are grouped as by following group: 10~20 weight of strophanthus divaricatus
Part, 4~8 parts by weight of radix scutellariae, 6~10 parts by weight of 6~10 parts by weight of balsam pear, 4~8 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra, preparation method
Include: to be concentrated after being extracted after above-mentioned each component co-grinding with 20~30% alcohol refluxs, obtains concentrate, which is passed through
AB-8 macroporous absorbent resin isolates and purifies to obtain eluent, by the eluent through heating in vacuum concentration and vacuum freeze drying to get
The herbal composite with acne-removal function.
In a preferred embodiment of the invention, 14~18 parts by weight of strophanthus divaricatus, 4~6 parts by weight of radix scutellariae, balsam pear 7
7~9 parts by weight of~9 parts by weight, 5~7 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra.
It is further preferred that its raw material components is grouped as by following group: 15 parts by weight of strophanthus divaricatus, 6 parts by weight of radix scutellariae, balsam pear
8 parts by weight of 8 parts by weight, 6 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra.
A kind of preparation method of the above-mentioned herbal composite with acne-removal function, includes the following steps:
(1) each component is weighed by weight:；
(2) by after above-mentioned each component co-grinding, 2~3 times are extracted with 20~30% alcohol refluxs of 6~8 times of volumes, often
It is divided into 1.5~2.5h between secondary refluxing extraction, is all filtered after each refluxing extraction, remerges filtrate, recycles ethyl alcohol, decompression
It is concentrated to give concentrate；
(3) above-mentioned concentrate is isolated and purified with AB-8 macroporous absorbent resin, specifically: by the above-mentioned concentration of 7~8BV
Liquid by being equipped with the chromatographic column of AB-8 macroporous absorbent resin, is saturated resin adsorption with the flow velocity of 2.5~3BV/h, then with 5~
The distilled water of 7BV is rinsed with the flow velocity of 2.5~3.5BV/h to colourless, then with 50~70% ethyl alcohol of 5~6BV with 2.5~3BV/
The flow velocity of h elutes, and obtains eluent；
(4) above-mentioned eluent is concentrated under the conditions of -0.04~-0.10MPa, 45~60 DEG C, recycles ethyl alcohol, vacuum refrigeration
Drying is to get the herbal composite with acne-removal function.
In a preferred embodiment of the invention, the step (2) are as follows: after above-mentioned each component co-grinding, with 8
30% alcohol reflux of times volume extracts 2 times, is divided into 2h between each refluxing extraction, is all filtered after each refluxing extraction,
Filtrate is remerged, ethyl alcohol is recycled, concentrate is concentrated under reduced pressure to obtain；
In a preferred embodiment of the invention, the step (3) are as follows: by above-mentioned concentrate AB-8 macroporous absorption
Resin is isolated and purified, specifically: by the above-mentioned concentrate of 7BV with the flow velocity of 2.5BV/h by being equipped with AB-8 macroporous absorption tree
The chromatographic column of rouge, is saturated resin adsorption, is then rinsed with the flow velocity of 3BV/h to colourless, then with 5BV's with the distilled water of 6BV
50% ethyl alcohol is eluted with the flow velocity of 2.5BV/h, obtains eluent.
In a preferred embodiment of the invention, the step (4) are as follows: by above-mentioned eluent -0.05~-
0.09MPa, it is concentrated under the conditions of 50~60 DEG C, recycling ethyl alcohol, vacuum freeze drying are to get the Chinese herbal medicine with acne-removal function
Composition.
A kind of above-mentioned herbal composite with acne-removal function is preparing the application in cosmetics.
The beneficial effects of the present invention are:
1, it was found that if reusing clearing damp medicine, helping with cool blood when external curing acne species discosmetic dermatosis in research
Medicine, curative effect is very significant, thus the preferred strophanthus divaricatus of herbal composite of the invention, balsam pear and radix scutellariae, to reach clearing heat and expelling damp
Purpose, can make inflammatory papule, tubercle prompt resolution, and Radix Salviae Miltiorrhizae and radix paeoniae rubra then can cooling and activating blood, to therapeutic effect
Consolidation is dispelled with acne print, is played an important role.
2, herbal composite of the invention has the features such as using safe, significant curative effect, uneasy to recur, can avoid long-term
The side effect generated when using hormone and antibiotic, at the same conditioning skin state, improve Endocrine Levels on also have it is certain
Positive effect；
3, herbal composite of the invention has apparent inhibitory effect to the common bacteria in acne, and clinical effectiveness is aobvious
It writes, can fundamentally kill pathogenic germ, accelerate skin metabolism, not stay acne print, it is uneasy to recur.
4, preparation method process of the invention is simple, and equipment requirement is low, is suitble to industrialized production.
Specific embodiment
Technical solution of the present invention is further explained and described below by way of specific embodiment.
Embodiment 1
(1) each component is weighed by weight: 15 parts by weight of strophanthus divaricatus, 6 parts by weight of radix scutellariae, 8 parts by weight of balsam pear, 6 weight of Radix Salviae Miltiorrhizae
Measure part and 8 parts by weight of radix paeoniae rubra；
(2) it by after above-mentioned each component co-grinding, is extracted 2 times with 30% alcohol reflux of 8 times of volumes, each refluxing extraction
Between be divided into 2h, be all filtered after each refluxing extraction, remerge filtrate, recycled ethyl alcohol, concentrate is concentrated under reduced pressure to obtain；
(3) above-mentioned concentrate is isolated and purified with AB-8 macroporous absorbent resin, specifically: by the above-mentioned concentrate of 7BV
With the flow velocity of 2.5BV/h by the chromatographic column equipped with AB-8 macroporous absorbent resin, it is saturated resin adsorption, then uses the steaming of 6BV
Distilled water is rinsed with the flow velocity of 3BV/h to colourless, then is eluted with 50% ethyl alcohol of 5BV with the flow velocity of 2.5BV/h, and eluent is obtained；
(4) above-mentioned eluent is concentrated under the conditions of -0.05~-0.09MPa, 50~60 DEG C, recycles ethyl alcohol, vacuum refrigeration
Drying is to get the herbal composite with acne-removal function.
Bacteriostatic activity test
Sample solution is prepared: the freeze-drying sample of herbal composite is dissolved with dimethylformamide (DMF), is configured to maximum
Concentration is 0.97g/ml.
Method: it (is stored in kitchen meat culture solution, paraffin envelope from propionibacterium acnes (P.acne) strain is taken out in refrigerator
Mouthful), 1ml strain is drawn with aseptic straw and is put into sterilized 1% THIOGLYCOLLIC ACID salt culture solution test tube, is placed in 37 in anaerobic jar
DEG C culture 48h.Staphylococcus aureus is taken out simultaneously, staphylococcus epidermis (storage and brucella broth culture solution) is inoculated in inclined-plane
Culture solution, 37 DEG C of cultures are for 24 hours.By the propionibacterium acnes in logarithmic growth phase, staphylococcus aureus, staphylococcus epidermis
It is diluted to 0.5 maxwell reduced turbidity with 0.9% physiology, is inoculated in equipped in sterilized culture solution test tube, adjustment bacterium solution is dense eventually
Degree is 103~104CFU/ml draws a certain amount of sample solution and is added in the culture solution test tube containing bacterium solution, every kind of three set level of medicine
Row pipe.Three control tubes: culture solution containing 1%DMF separately are set, are inoculated with propionibacterium acnes, golden yellow grape respectively in culture solution
Coccus, staphylococcus epidermis, the ultimate density 10 for making bacterium solution3~104CFU/ml.Propionibacterium acnes, which are placed in anaerobic jar, (to be detested
Oxygen culture gas: N280%, CO210%, H210%) it observes after 37 DEG C of culture 48h as a result, staphylococcus aureus, epidermis Portugal
Result is observed in the 37 DEG C of cultures of grape coccus afterwards for 24 hours.It the results are shown in Table 1 according to the bacteriostatic test of maximum concentration.
1 bacteriostatic activity test result of table
Control group " +++ " be it is not antibacterial, the part " ⊥ " is antibacterial, and "-" is antibacterial
Clinical effectiveness:
According to Ministry of Public Health's dermatosis treating medicine guideline of clinical investigations, anti-acne effect is counted after 140 person-times of uses.
Criterion of therapeutical effect: cure: original skin lesion is subsided.In addition to something lost has slight pigmentation, no new skin lesion generates face invariably
Suitable sense；Effective: it is substantially reduced that 70% or more of inflammatory papules recession still has a little new fash that subjective symptoms occurs；It improves: scorching
30% or more disease property papule recession still has new fash to occur, but inflammatory papules mitigate subjective symptoms mitigation earlier above；It is invalid: treatment
Front and back is without significant change or exacerbation.
Treatment results: effective 52 of 56 are cured and are improved 24, invalid 8.Total effective rate is 94.29%.
The pharmacological results that the present invention is done show propionibacterium acnes, staphylococcus aureus, staphylococcus epidermis
It is more sensitive to the active constituent of the herbal composite.Clinical effectiveness is significant.Therefore, herbal composite of the invention is used
In preventing and treating whelk, effect is obvious, can fundamentally inhibit or kill pathogenic germ, accelerate skin metabolism,
Acne print is not stayed, it is uneasy to recur, it is a kind of safety, good traditional Chinese medicine for external application can be applied in the preparation of acne-eliminating cosmetic.
Skilled person will appreciate that still being able to obtain when technical solution of the present invention changes in parameters described below
Same as the previously described embodiments or similar technical effect:
A kind of herbal composite with acne-removal function, raw material are grouped as by following group: 10~20 weight of strophanthus divaricatus
Part, 4~8 parts by weight of radix scutellariae, 6~10 parts by weight of 6~10 parts by weight of balsam pear, 4~8 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra, preparation method
Include: to be concentrated after being extracted after above-mentioned each component co-grinding with 20~30% alcohol refluxs, obtains concentrate, which is passed through
AB-8 macroporous absorbent resin isolates and purifies to obtain eluent, by the eluent through heating in vacuum concentration and vacuum freeze drying to get
The herbal composite with acne-removal function.
In a preferred embodiment of the invention, 14~18 parts by weight of strophanthus divaricatus, 4~6 parts by weight of radix scutellariae, balsam pear 7
7~9 parts by weight of~9 parts by weight, 5~7 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra.
A kind of preparation method of the above-mentioned herbal composite with acne-removal function, includes the following steps:
(1) each component is weighed by weight:；
(2) by after above-mentioned each component co-grinding, 2~3 times are extracted with 20~30% alcohol refluxs of 6~8 times of volumes, often
It is divided into 1.5~2.5h between secondary refluxing extraction, is all filtered after each refluxing extraction, remerges filtrate, recycles ethyl alcohol, decompression
It is concentrated to give concentrate；
(3) above-mentioned concentrate is isolated and purified with AB-8 macroporous absorbent resin, specifically: by the above-mentioned concentration of 7~8BV
Liquid by being equipped with the chromatographic column of AB-8 macroporous absorbent resin, is saturated resin adsorption with the flow velocity of 2.5~3BV/h, then with 5~
The distilled water of 7BV is rinsed with the flow velocity of 2.5~3.5BV/h to colourless, then with 50~70% ethyl alcohol of 5~6BV with 2.5~3BV/
The flow velocity of h elutes, and obtains eluent；
(4) above-mentioned eluent is concentrated under the conditions of -0.04~-0.10MPa, 45~60 DEG C, recycles ethyl alcohol, vacuum refrigeration
Drying is to get the herbal composite with acne-removal function.
The foregoing is only a preferred embodiment of the present invention, the range that the present invention that therefore, it cannot be limited according to is implemented, i.e.,
Equivalent changes and modifications made in accordance with the scope of the invention and the contents of the specification should still be within the scope of the present invention.
Claims (7)
1. a kind of herbal composite with acne-removal function, it is characterised in that: its raw material is grouped as by following group: strophanthus divaricatus 10
~20 parts by weight, 4~8 parts by weight of radix scutellariae, 6~10 parts by weight of 6~10 parts by weight of balsam pear, 4~8 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra,
Preparation method includes the following steps:
(1) each component is weighed by weight；
(2) it by after above-mentioned each component co-grinding, is extracted 2~3 times with 20~30% alcohol refluxs of 6~8 times of volumes, is returned every time
Stream is divided into 1.5~2.5h between extracting, and is all filtered after each refluxing extraction, remerges filtrate, recycles ethyl alcohol, is concentrated under reduced pressure
Obtain concentrate；
(3) above-mentioned concentrate is isolated and purified with AB-8 macroporous absorbent resin, specifically: by the above-mentioned concentrate of 7~8BV with
The flow velocity of 2.5~3BV/h is saturated resin adsorption, then with 5~7BV by the chromatographic column equipped with AB-8 macroporous absorbent resin
Distilled water rinsed with the flow velocity of 2.5~3.5BV/h to colourless, then with 50~70% ethyl alcohol of 5~6BV with 2.5~3BV/h's
Flow velocity elution, obtains eluent；
(4) above-mentioned eluent is concentrated under the conditions of -0.04~-0.10MPa, 45~60 DEG C, recycling ethyl alcohol, vacuum refrigeration are dry
It is dry to get the herbal composite with acne-removal function, the herbal composite is external preparation.
2. a kind of herbal composite with acne-removal function as described in claim 1, it is characterised in that: strophanthus divaricatus 14~18
Parts by weight, 4~6 parts by weight of radix scutellariae, 7~9 parts by weight of 7~9 parts by weight of balsam pear, 5~7 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra.
3. a kind of herbal composite with acne-removal function as claimed in claim 2, it is characterised in that: its raw material components by
Following group is grouped as: 15 parts by weight of strophanthus divaricatus, 6 parts by weight of radix scutellariae, 8 parts by weight of 8 parts by weight of balsam pear, 6 parts by weight of Radix Salviae Miltiorrhizae and radix paeoniae rubra.
4. a kind of herbal composite with acne-removal function as described in claim 1, it is characterised in that: the step (2)
Are as follows: it after above-mentioned each component co-grinding, is extracted 2 times with 30% alcohol reflux of 8 times of volumes, is divided between each refluxing extraction
2h is filtered after each refluxing extraction, remerges filtrate, recycles ethyl alcohol, concentrate is concentrated under reduced pressure to obtain.
5. a kind of herbal composite with acne-removal function as described in claim 1, it is characterised in that: the step (3)
Are as follows: above-mentioned concentrate is isolated and purified with AB-8 macroporous absorbent resin, specifically: by the above-mentioned concentrate of 7BV with 2.5BV/h
Flow velocity by being equipped with the chromatographic column of AB-8 macroporous absorbent resin, be saturated resin adsorption, then with the distilled water of 6BV with 3BV/
The flow velocity of h is rinsed to colourless, then is eluted with 50% ethyl alcohol of 5BV with the flow velocity of 2.5BV/h, and eluent is obtained.
6. a kind of herbal composite with acne-removal function as described in claim 1, it is characterised in that: the step (4)
Are as follows: above-mentioned eluent is concentrated under the conditions of -0.05~-0.09MPa, 50~60 DEG C, recycles ethyl alcohol, vacuum freeze drying, i.e.,
Obtain the herbal composite with acne-removal function.
7. the herbal composite in a kind of claim 1 to 6 described in any claim with acne-removal function is in preparationization
Application in cosmetic.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461850A (en) * | 2008-03-30 | 2009-06-24 | 郑沛 | Application of Chinese medicinal composition in preparing medicament for treating acne |
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| CN101336879A (en) * | 2008-08-07 | 2009-01-07 | 北京昂立达技术有限责任公司 | Art pumpkin extract and use as anti-corrosive bacteria inhibitor and anti acne agent in cosmetics |
| CN103432244B (en) * | 2013-09-11 | 2016-01-20 | 余红波 | A kind of compositions with acne-removal function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101461850A (en) * | 2008-03-30 | 2009-06-24 | 郑沛 | Application of Chinese medicinal composition in preparing medicament for treating acne |
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Address after: 361000 No. 1999, Guan Kou Middle Road, Jimei District, Xiamen, Fujian. Applicant after: Xiamen Medical College Address before: 361000 No. 8, Qian Pu Yan Road, Siming District, Xiamen, Fujian. Applicant before: Xiamen Medical College |
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