CN101700257B - Pomegranate peel polyphenol antibiosis and antiphlogosis effervescent tablet as well as preparation method and application thereof - Google Patents
Pomegranate peel polyphenol antibiosis and antiphlogosis effervescent tablet as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a pomegranate peel polyphenol antibiosis and antiphlogosis effervescent tablet as well as a preparation method and application thereof. The effervescent tablet is mainly prepared by raw material of pomegranate peel polyphenol, and auxiliary materials of sodium hydrogen carbonate, citric acid or tartaric acid, lactose, polyvinylpyrrolidone, magnesium stearate and superfine silica powder, has the functions of clearing heat, eliminating dampness, killing bugs and stopping itching, has strong suppression effect to women vagina pathogenic bacteria, features high safety, good stability, no obvious toxic or side effect, or drug tolerance, and provides convenience in using, carrying and storing for patients, is used for curing such diseases as vaginitis, vulvitis and the like caused by various trichomonad, epiphyte and bacteria, and can be used as preventability medicine for long-term use.
Description
Technical field
The present invention relates to a kind of is the effervescent tablet and the application thereof of the externally used antimicrobial antiphlogistic activity of feedstock production with the pomegranate peel polyphenol.
Background technology
Gynaecopathia is meant that female genital system infects caused disease.Because the particularity of female sex organ, gynaecopathia has become adult female's common, frequently-occurring disease, and its clinical manifestation is varied, and the cause of disease is complicated and often with multiple severe complication, and women's live and work has been brought very big influence.In addition, along with the change of current environmental pollution, competitive pressure, work rhythm quickening, life style, gynaecopathia will continue to rise to some extent, and present rejuvenation, development trend becomes younger.According to World Health Organization (WHO) Chinese women's investigation is shown: the female patient of China 41% has colpitis in various degree.Married women, especially at farming and pastoral area and outlying district sickness rate up to more than 70%, fertility back women almost 100% suffers from a kind of gynecological inflammation at least.Along with the raising of people's living standard, the maturation of consumption idea, health care consciousness strengthen gradually, and removing original is the purpose consumer group with the treatment, with simple cleaning-nursing, to keep healthy be that the consumer group of purpose enlarges rapidly.
Treat gynecological inflammation clinically mainly based on Western medicine and Chinese medicine.Western medicine has erious adverse reaction, especially with Liver and kidney toxicity for very; Behind the stopped treatment, the characteristics of easy recurrence.And Chinese medicine is suitable for long-term prescription because side effect is little, has adapted to the gynecological inflammation characteristics of recurrence easily, and clinical practice is very extensive.Chinese patent 200610164376.1: a kind of effervescent tablet for the treatment of female vagina and its production and use, Chinese patent 200910113244.X: a kind of effervescent tablets for women and its production and use, the two is compound Chinese medicinal preparation, the extraction process complexity, and the composition of performance drug effect is also complicated.And the crude drug that the present invention relates to is traditional Uygur medicine medicinal herbs most in use extract, be further research on the basis of the preparation method of a patent 200810073030.X pericarpium granati total phenols and application, its composition is single, extraction process is simple, and the main component of performance drug effect is the tannin compounds, determined curative effect aspect the treatment gynecological inflammation.According to domestic and foreign literature, more based on the listing of the effervescent tablet of chemical medicine, but be that the effervescent tablet that primary raw material is made does not see that as yet any report is arranged with traditional Uygur medicine medicinal herbs most in use.The present invention is excavating and is putting in order on the basis of uighur medicine theory, in conjunction with Uygur's medical science and modern Chinese medicine scientific principle opinion, screens meticulously and redesigns, and develops Uygur's new drug prescription, improves the Uigurs medicine research level.
Summary of the invention
The objective of the invention is to, a kind of anti-inflammation external effervescent tablet and its production and use is provided, this effervescent tablet is by the crude drug pomegranate peel polyphenol, the external used medicine that adjuvant sodium bicarbonate, lactose, citric acid or tartaric acid, polyvinylpyrrolidone, magnesium stearate and micropowder silica gel are made.Through the pharmacodynamics test checking, have heat clearing and damp drying, killing parasites for relieving itching has powerful inhibitory action to vaginal pathogenic.Safe, good stability, no obvious toxic and side effects and drug resistance.Be used for the treatment of diseases such as the various infusorian of women, fungus, bacterial vaginitis, vulvitis, also can be used as preventative medicine life-time service simultaneously.
The pomegranate peel polyphenol anti-inflammation effervescent tablet of a kind of external of the present invention, this effervescent tablet is a radix to make 1000, is to be made by crude drug pomegranate peel polyphenol 60-720g, adjuvant sodium bicarbonate 50-300g, citric acid or tartaric acid 70-350g, lactose 160-700g, polyvinylpyrrolidone 2-50g, magnesium stearate 2-30g and micropowder silica gel 7-70g.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet, with per 1000 is radix, crude drug is preferred: pomegranate peel polyphenol 100-600g, adjuvant is preferred: sodium bicarbonate 80-200g, citric acid or tartaric acid 110-250g, lactose 220-540g, polyvinylpyrrolidone 5-25g, magnesium stearate 4-20g and micropowder silica gel 10-60g.
The preparation method of described pomegranate peel polyphenol anti-inflammation effervescent tablet follows these steps to carry out:
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, add 5-30 and doubly measure 50-70% ethanol warm macerating, temperature is 30-70 ℃, extracts each 1-3 hour 1-4 time, filter, decompression filtrate recycling ethanol through macroporous resin chromatography, adsorbs 6-12h with medicinal liquid, it is closely colourless earlier to be eluted to effluent with distilled water, reuse 40-80% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses the 60-140 mesh sieve, get pomegranate peel polyphenol, standby;
B, get adjuvant sodium bicarbonate, citric acid or tartaric acid, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the pomegranate peel polyphenol of step a, mixture and the lactose mix homogeneously of step b, with the anhydrous alcohol solution that contains the 2-15% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, the 14-20 mesh sieve is granulated, temperature 30-75 ℃ dry 1-4 hour, 14-20 mesh sieve granulate, add magnesium stearate, micropowder silica gel mix homogeneously, tabletting can obtain effervescent tablet;
Or will be divided into two parts behind the pomegranate peel polyphenol of step a and the adjuvant lactose mixing, make soft material with the 60-95% ethanol liquid that contains the 2-10% polyvinylpyrrolidone by normal pharmacy rule method with sodium bicarbonate, citric acid or tartaric acid respectively, the 14-20 mesh sieve is granulated, temperature 30-75 ℃ dry 1-4 hour, 14-20 mesh sieve granulate, add magnesium stearate, micropowder silica gel mix homogeneously, tabletting can obtain effervescent tablet.
Macroporous resin described in the step a is D101, D141, AB-8, HPD-100, HPD-300, HPD-450 or HPD-750 type macroporous resin.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is as the purposes of preparation treatment women by the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is as the purposes of preparation treatment women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis.
Pomegranate peel polyphenol anti-inflammation effervescent tablet of the present invention and its production and use, volume is little, carry, easy to use, storage period is long, overcome the lotion poor stability, the patient carries and store shortcomings such as inconvenience.Only need join during use in an amount of warm water, just disintegrate rapidly, than the high 5-6 of conventional tablet disintegration rate doubly.
The pharmacological action of pomegranate peel polyphenol anti-inflammation effervescent tablet of the present invention is:
Punica granatum L. polyphenol is the general name that a class has the polyphenol hydroxyl chemical compound, comprise multiple chemical compounds such as ellagitannin, gallotannin, ellagic acid, gallic acid, catechin, anthocyanidin, chlorogenic acid, ferulic acid, mainly be present in the Pericarpium Granati, content is about the 10-20% of its dry mass.Have multiple physiology and pharmacological actions such as antioxidation, defying age, anti-cancer and cancer-preventing, antibiotic, moistening skin beautifying, blood pressure lowering and prevention cardiovascular and cerebrovascular disease.Compare with grape polyphenols with tea polyphenols, the non-oxidizability and the antibacterial activity of Punica granatum L. polyphenol are higher, and study of anti-atherogenic effect is more remarkable.
Pomegranate peel polyphenol anti-inflammation effervescent tablet extracorporeal bacteria inhibitor test research of the present invention:
Quality Control bacterial strain large intestine Erichsen bacterium ATCC25922, staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853 and Candida albicans ATCC10231
Culture medium: MH agar culture medium, MH broth bouillon, husky Bao Luoshi agar culture medium, husky Bao Luoshi broth bouillon, produce by sky, Hangzhou and microorganism reagent company limited.
Experimental technique
(1) minimum inhibitory concentration (M1C) is measured: adopt agar dilution
The medicinal liquid that is subjected to reagent anti-inflammation external effervescent tablet variable concentrations of getting pretreatment quantitatively adds in the corresponding plate, 20,10,5,2.5,1.25,0.625,0.313mg/mL with the quantitative pour plate of the husky Bao Luoshi agar culture medium of MH/, the final concentration of given the test agent is respectively:.Get the above-mentioned various test organismss that the husky Bao Luoshi meat soup of MH/ liquid is cultivated respectively, being configured to concentration with normal saline is 1 * 10
7The bacterium liquid of CFU/ml, every some inoculation 5 μ l, total number of bacteria is about 0.5 * 10
5CFU establishes simultaneously and does not contain medicine but the inoculation and the normal control of medicine group equivalent test organisms, puts 37 ℃ and cultivates observed result behind the 24h, and the high dilution of the medicine of not growing with bacterial strain of the same race is its MIC value.
(2) minimum bactericidal concentration (MBC) is measured: adopt the test tube doubling dilution
Each test organisms is inoculated broth bouillon respectively, cultivate 16～18h, be diluted to 1 * 10 with normal saline then for 37 ℃
7CFU/ml.20,10,5,2.5,1.25,0.625,0.313mg/ml with corresponding broth bouillon dilution given the test agent, final concentration is respectively:.The medicinal liquid that is subjected to reagent anti-inflammation external effervescent tablet variable concentrations of getting pretreatment respectively adds in the corresponding sterile test tube, every pipe 2ml (control tube adds the 2ml culture medium).Inoculate above-mentioned each test organisms liquid 0.1ml respectively, observed result behind 37 ℃ of cultivation 18h～24h, be inoculated in the corresponding agar culture medium to draw 0.1ml in the culture tube of asepsis growth respectively, cultivating the 18h-24h observed results for 37 ℃, is minimum bactericidal concentration (MBC) with the minimum reagent substrate concentration that is subjected to of asepsis growth.The results are shown in Table 1.
Table 1 extracorporeal bacteria inhibitor test result
Conclusion: pomegranate peel polyphenol anti-inflammation effervescent tablet all has in various degree antibacterial and bactericidal action to staphylococcus aureus, escherichia coli, bacillus pyocyaneus, Bacillus proteus, klebsiella pneumoniae, Candida albicans, streptococcus faecalis, especially the inhibitory action to staphylococcus aureus and bacillus pyocyaneus is the strongest, and its minimal inhibitory concentration MIC is respectively 0.313mg/mL, 2.5mg/mL.
Pomegranate peel polyphenol anti-inflammation effervescent lamellar body giving drugs into nose of the present invention is imitated and is learned experimental study:
Pityrol ointment, lot number 080903, Kang Hua pharmaceutcal corporation, Ltd in Xi'an provides; Dimethylbenzene, Xi'an chemical reagent factory, lot number 070704; Carrageenin, U.S. Sigma company product, 070103;
Kunming kind white mice, Wistar rat, male and female half and half.
(1) mice caused by dimethylbenzene xylene ear swelling test
50 of Kunming mouses, male and female half and half are divided into 5 groups at random by body weight, are respectively substrate matched group, Pityrol ointment positive controls and are subjected to high, medium and low 3 the dosage groups of reagent effervescent tablets for women; Every group of positive and negative two sides of the equal auris dextra of mice is coated with relative medicine, and 0.1ml/, positive controls only is coated with Pityrol ointment 0.1g/; Left side ear contrast, more than respectively organize coating 2 times every day, 3d continuously.30min after not inferior administration, the positive and negative two sides of auris dextra is coated with 100% caused by dimethylbenzene xylene inflammation, and 0.02ml/ only takes off cervical vertebra and puts to death behind the 30min, cut ears along the auricle baseline, is that the 8mm card punch is laid auricle at the same position of left and right ear respectively with diameter.Analytical balance is accurately weighed, and is the swelling degree with two auricle weight differences, respectively organizes the swelling degree, and calculates and respectively organize inhibitory rate of intumesce.
Table 2 is subjected to the influence (x ± SD) of reagent xylol induced mice auricle edema
Annotate: compare with the substrate matched group
*P＜0.05,
*P＜0.01.
By the result of the test of table 2 as seen: positive controls, be subjected to the swelling degree of the middle and high dosage group of reagent and matched group that utmost point significant difference is more all arranged.Show to be subjected to the middle and high dosage group of reagent can suppress the mice auricle swelling that is caused by dimethylbenzene, its suppression ratio is respectively 35.9% and 46.5%, shows certain dose-effect relationship
(2) carrageenin causes the sufficient sole of the foot (pawl) swelling test
50 of Wistar rats, male and female half and half are divided into 5 groups at random by body weight, are respectively substrate matched group, Pityrol ointment positive controls and are subjected to high, medium and low 3 the dosage groups of reagent effervescent tablets for women, 10 every group; The right back foot of each administration group rat is smeared administration, 0.1ml/100g, 2 times/d, continuous 3d.Behind the last administration 30min, measure right back sufficient sole of the foot thickness, the right back sufficient sole of the foot of every then Mus is injected 1% carrageenin solution, 50 μ l, and the injection back is measured 1h, 2h, 3h, 4h, 6h with micrometer callipers and respectively organized the right back sufficient sole of the foot thickness of rat, represents the swelling degree according to difference before and after its injection.
Table 3 is subjected to the reagent on Carrageenan to cause the influence (x ± SD) of rat paw edema
As can be seen from Table 3: be subjected to the basic, normal, high dosage of reagent (0.025g/kg, 0.05g/kg, 0.10g/kg) group and positive controls significant difference to be arranged causing scorching back 0.5h, illustrate to be subjected to reagent can suppress rat paw edema (P＜0.05) due to the carrageenin than the substrate matched group.1,2,4 and 6h be subjected to the swelling degree of each dosage group of reagent and matched group comparison that though reducing tendency is arranged, do not have statistical significance.
Conclusion: the pharmacodynamics test shows in the body, and the anti-inflammation effervescent tablet can suppress the mice auricle swelling that caused by dimethylbenzene, suppresses the rat paw edema that caused by carrageenin, and with matched group significant difference (P＜0.05) is arranged relatively.Show that by internal and external test effervescent tablets for women has the excellent antibiotic antiinflammatory action.
In sum, pomegranate peel polyphenol anti-inflammation effervescent tablet is as the All Pure Nature national medicine, and the collection treatment is the external use gynaecological good medicine of one with prevention.
The specific embodiment
Embodiment 1:(is a radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, and adding 5 times of amount concentration is that 30 ℃ of warm macerating of 50% ethanol temperature extract 3 hours time 1 time, filter, decompression filtrate recycling ethanol, with medicinal liquid through AB-8 type macroporous resin chromatography, absorption 6h, it is closely colourless to be eluted to effluent with distilled water earlier, reuse 40% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses 60 mesh sieves, get pomegranate peel polyphenol 60g, standby;
B, get adjuvant sodium bicarbonate 50g, citric acid 70g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 160g mix homogeneously of step b, with the anhydrous alcohol solution that contains 2% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 14 mesh sieves are granulated, 30 ℃ of dryings of temperature 1 hour, 14 mesh sieve granulate add magnesium stearate 4g, micropowder silica gel 10g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse;
Or will be divided into two parts behind the powder of step a and the adjuvant lactose 160g mixing, respectively with sodium bicarbonate 50g, citric acid 70g with the 60% ethanol liquid that contains 2% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 14 mesh sieves are granulated, 30 ℃ of dryings of temperature 1 hour, 14 mesh sieve granulate add magnesium stearate 4g, micropowder silica gel 10g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
Embodiment 2 (is radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, and adding 10 times of amount concentration is that 50 ℃ of warm macerating of 60% ethanol temperature extract each 1 hour 4 times, filter, decompression filtrate recycling ethanol, with medicinal liquid through D101 type macroporous resin chromatography, absorption 12h, it is closely colourless to be eluted to effluent with distilled water earlier, reuse 60% ethanol elution, be collected into eluent colourless till, concentrate, after 50 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses 80 mesh sieves, get pomegranate peel polyphenol 120g, standby;
B, get adjuvant sodium bicarbonate 80g, tartaric acid 110g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 220g mix homogeneously of step b, with the anhydrous alcohol solution that contains 6% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 16 mesh sieves are granulated, 40 ℃ of dryings of temperature 3 hours, 16 mesh sieve granulate add magnesium stearate 4g and micropowder silica gel 10g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse;
Or will be divided into two parts behind the powder of step a and the adjuvant lactose 220g mixing, respectively with sodium bicarbonate 80g, tartaric acid 110g with the 80% ethanol liquid that contains 4% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 16 mesh sieves are granulated, 50 ℃ of dryings of temperature 4 hours, 16 mesh sieve granulate add magnesium stearate 4g and micropowder silica gel 10g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
Embodiment 3 (is radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, and adding 15 times of amount concentration is that 50 ℃ of warm macerating of 60% ethanol temperature extract each 2 hours 2 times, filter, decompression filtrate recycling ethanol, with medicinal liquid through HPD300 type macroporous resin chromatography, absorption 8h, it is closely colourless to be eluted to effluent with distilled water earlier, reuse 60% ethanol elution, be collected into eluent colourless till, concentrate, after 55 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses 80 mesh sieves, get pomegranate peel polyphenol 315g, standby;
B, get adjuvant sodium bicarbonate 150g, citric acid 200g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 450g mix homogeneously of step b; With the anhydrous alcohol solution that contains 8% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 18 mesh sieves are granulated, 60 ℃ of dryings of temperature 2 hours, 18 mesh sieve granulate, add magnesium stearate 15g, micropowder silica gel 40g mix homogeneously, tabletting can obtain effervescent tablet, and this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse;
Or will be divided into two parts behind the powder of step a and the adjuvant lactose mixing, respectively with sodium bicarbonate 150g, citric acid 200g with the 80% ethanol liquid that contains 8% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 18 mesh sieves are granulated, 60 ℃ of dryings of temperature 2 hours, 18 mesh sieve granulate add magnesium stearate 15g, micropowder silica gel 40g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
Embodiment 4 (is radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, adds 60 ℃ of warm macerating of 20 times of amount 65% ethanol and extracts each 2 hours 3 times, filter, decompression filtrate recycling ethanol, with medicinal liquid through HPD-100 type macroporous resin chromatography, absorption 10h, it is closely colourless to be eluted to effluent with distilled water earlier, reuse 70% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses 120 mesh sieves, get pomegranate peel polyphenol 500g, standby;
B, get adjuvant sodium bicarbonate 200g, tartaric acid 250g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 540g mix homogeneously of step b; Make soft material according to a conventional method with the anhydrous alcohol solution that contains 12% polyvinylpyrrolidone, 16 mesh sieves are granulated, 70 ℃ of temperature dryings 3 hours, 16 mesh sieve granulate, add magnesium stearate 20g, micropowder silica gel 60g mix homogeneously, tabletting can obtain effervescent tablet, and this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Or will be divided into two parts behind the powder of step a and the adjuvant lactose 540g mixing, respectively with sodium bicarbonate 200g, tartaric acid 250g with the 80% ethanol liquid that contains 7% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 16 mesh sieves are granulated, 70 ℃ of dryings of temperature 3 hours, and 16 mesh sieve granulate, add magnesium stearate 20g, micropowder silica gel 60g mix homogeneously, the pharmaceutical methods tabletting is checked routinely, packing, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
Embodiment 5 (is radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, adding 30 times of amount concentration is 70% ethanol warm macerating, temperature is 70 ℃, extracts each 1 hour 4 times, filter, decompression filtrate recycling ethanol through the HPD-450 macroporous resin chromatography, adsorbs 12h with medicinal liquid, it is closely colourless earlier to be eluted to effluent with distilled water, reuse 80% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses 140 mesh sieves, get pomegranate peel polyphenol 720g, standby;
B, get adjuvant sodium bicarbonate 300g, tartaric acid 350g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 700g mix homogeneously of step b, with the anhydrous alcohol solution that contains 15% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 20 mesh sieves are granulated, 75 ℃ of dryings of temperature 4 hours, 20 mesh sieve granulate add magnesium stearate 30g, micropowder silica gel 70g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Or will be divided into two parts behind the powder of step a and the adjuvant lactose 700g mixing, make soft material with the 95% ethanol liquid that contains 10% polyvinylpyrrolidone by normal pharmacy rule method with sodium bicarbonate 300g, tartaric acid 350g respectively, 20 mesh sieves are granulated, 75 ℃ of dryings of temperature 4 hours, 20 mesh sieve granulate add magnesium stearate, micropowder silica gel mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
Embodiment 6 (is radix to make 1000)
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, and adding 25 times of amount concentration is 65% ethanol warm macerating, and temperature is 60 ℃, extracts 3 times, each 3 hours, filter decompression filtrate recycling ethanol, medicinal liquid through the D141 macroporous resin chromatography, is adsorbed 10h, and it is closely colourless to be eluted to effluent with distilled water earlier, reuse 70% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, concentrating under reduced pressure dry extract powder, pulverize, cross 120 mesh sieves, get pomegranate peel polyphenol 650g, standby;
B, get adjuvant sodium bicarbonate 220g, citric acid 280g, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the powder of step a, mixture and the lactose 600g mix homogeneously of step b, with the anhydrous alcohol solution that contains 13% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, 16 mesh sieves are granulated, 65 ℃ of dryings of temperature 3 hours, 16 mesh sieve granulate add magnesium stearate 24g, micropowder silica gel 55g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Or will be divided into two parts behind the powder of step a and the adjuvant lactose 600g mixing, make soft material with the 75% ethanol liquid that contains 9% polyvinylpyrrolidone by normal pharmacy rule method with sodium bicarbonate 220g, citric acid 280g respectively, 16 mesh sieves are granulated, 65 ℃ of dryings of temperature 3 hours, 16 mesh sieve granulate add magnesium stearate 24g, micropowder silica gel 55g mix homogeneously, tabletting, can obtain effervescent tablet, this effervescent tablet is Lycoperdon polymorphum Vitt or lark ellipse.
Described pomegranate peel polyphenol anti-inflammation effervescent tablet is treated the purposes of women by the preventative medicine of infusorian, fungus, bacterial vaginitis, vulvitis as preparation treatment women by the purposes of the external used medicine of infusorian, fungus, bacterial vaginitis, vulvitis or as preparation.
Using method: 1 effervescent tablet is got final product with 50ml warm water dissolving afterflush pudendum, once a day, each a slice.
The present invention is Lycoperdon polymorphum Vitt or lark ellipse effervescent tablet, has heat clearing and damp drying, and killing parasites for relieving itching has powerful inhibitory action to vaginal pathogenic, safe, good stability, no obvious toxic and side effects and drug resistance.Be used for the treatment of diseases such as the various infusorian of women, fungus, bacterial vaginitis, vulvitis, also can be used as preventative medicine life-time service simultaneously.
Claims (2)
1. external pomegranate peel polyphenol anti-inflammation effervescent tablet, it is characterized in that this effervescent tablet is a radix to make 1000, be to be made by crude drug pomegranate peel polyphenol 60-720g, adjuvant sodium bicarbonate 50-300g, citric acid or tartaric acid 70-350g, lactose 160-700g, polyvinylpyrrolidone 2-50g, magnesium stearate 2-30g and micropowder silica gel 7-70g, concrete operations follow these steps to carry out:
A, at first the weighting raw materials Pericarpium Granati is ground into coarse powder, adding 5-30, doubly to measure concentration be 50-70% ethanol warm macerating, temperature 30-70 ℃, extract each 1-3 hour 1-4 time, filter, decompression filtrate recycling ethanol through macroporous resin chromatography, adsorbs 3-12h with medicinal liquid, it is closely colourless earlier to be eluted to effluent with distilled water, reuse 40-80% ethanol elution, be collected into eluent colourless till, concentrate, after 60 ℃ of temperature are removed ethanol, the dry extract powder that gets of concentrating under reduced pressure is pulverized, and crosses the 60-140 mesh sieve, obtain pomegranate peel polyphenol, standby;
B, get adjuvant sodium bicarbonate, citric acid or tartaric acid, pulverize separately is crossed 100 mesh sieves, and is standby;
C, with the pomegranate peel polyphenol of step a, mixture and the lactose mix homogeneously of step b, with the anhydrous alcohol solution that contains the 2-15% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, the 14-20 mesh sieve is granulated, temperature 30-75 ℃ dry 1-4 hour, 14-20 mesh sieve granulate, add magnesium stearate, micropowder silica gel mix homogeneously, tabletting can obtain effervescent tablet;
Or will be divided into two parts behind the pomegranate peel polyphenol of step a and the adjuvant lactose mixing, respectively with sodium bicarbonate, citric acid or tartaric acid with the 60-95% ethanol liquid that contains the 2-10% polyvinylpyrrolidone routinely pharmaceutical methods make soft material, the 14-20 mesh sieve is granulated, temperature 30-75 ℃ dry 1-4 hour, 14-20 mesh sieve granulate, add magnesium stearate, micropowder silica gel mix homogeneously, tabletting can obtain effervescent tablet.
2. according to the described pomegranate peel polyphenol anti-inflammation of claim 1 effervescent tablet, it is characterized in that macroporous resin described in the step a is D101, D141, AB-8, HPD-100, HPD-300, HPD-450 or HPD-750 type macroporous resin.
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| CN103155953A (en) * | 2011-12-19 | 2013-06-19 | 陕西恒田化工有限公司 | Pomegranate peel polyphenol plant fungicide and application and preparation method |
| CN102755360A (en) * | 2012-07-24 | 2012-10-31 | 西华大学 | Method for extracting pomegranate peel polyphenols from pomegranate peel |
| CN102823874A (en) * | 2012-09-21 | 2012-12-19 | 耿福能 | Pomegranate effervescent tablets and preparation method thereof |
| CN102861116B (en) * | 2012-10-18 | 2015-02-25 | 中国科学院新疆理化技术研究所 | Method for preparing pericarpium granati polyphenol gel for treating gynecological inflammation |
| CN102949373B (en) * | 2012-11-04 | 2014-07-09 | 耿福能 | Foot bath effervescent tablets and preparation method thereof |
| CN102940595A (en) * | 2012-12-04 | 2013-02-27 | 中国科学院新疆理化技术研究所 | Mouthwash with functions of antibiosis, antiphlogosis and deodorization, and preparation method and use thereof |
| CN102973617B (en) * | 2012-12-11 | 2015-07-29 | 青岛文创科技有限公司 | A kind of extracting method of pomegranate peel polyphenol |
| CN104688786B (en) * | 2013-12-05 | 2018-06-05 | 舒梅 | A kind of externally-applied medicinal composition and its preparation method and application |
| CN104688648B (en) * | 2013-12-05 | 2017-12-26 | 耿福能 | A kind of composition for oral cavity and preparation method thereof |
| CN104825504A (en) * | 2015-04-28 | 2015-08-12 | 中国科学院新疆理化技术研究所 | Anti-inflammatory application of pomegranate flower polyphenol |
| CN105878306A (en) * | 2016-04-11 | 2016-08-24 | 浙江中医药大学 | Preparation method of pomegranate peel polyphenols |
| CN110292582A (en) * | 2019-08-08 | 2019-10-01 | 枣庄学院 | A kind of ellagic acid-montmorillonite effervescent agent and preparation method thereof |
| CN112494619A (en) * | 2020-08-27 | 2021-03-16 | 新疆医科大学 | Traditional Chinese medicine effervescent tablet for treating cervical cancer and preparation method and application thereof |
| CN112999177A (en) * | 2021-01-30 | 2021-06-22 | 成都大学 | Chinese herbal medicine bacteriostatic effervescent tablet containing ligusticum wallichii extract and preparation method thereof |
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