CN102755360A - Method for extracting pomegranate peel polyphenols from pomegranate peel - Google Patents
Method for extracting pomegranate peel polyphenols from pomegranate peel Download PDFInfo
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Abstract
The invention belongs to the field of extraction from plants and particularly relates to a method for extracting pomegranate peel polyphenols from pomegranate peel. The method has the advantages of simplicity and convenience in operation, and high extraction efficiency. Particularly, the method comprises the steps of taking water as a solvent, extracting with microwaves to obtain coarse extract of pomegranate peel polyphenols, separating with macroporous resin, concentrating under reduced pressure, and vacuum freeze-drying to obtain the pomegranate peel polyphenols. The obtained coarse extract of pomegranate peel polyphenols and pomegranate peel polyphenols are higher in the total content of polyphenols, and the yield of the coarse extract reaches 40-50%, so that a new choice is provided for obtaining of pomegranate peel polyphenols.
Description
Technical field
The invention belongs to field of plant extraction, be specifically related to a kind of method of extracting pomegranate peel polyphenol from Pericarpium Granati.
Background technology
Pericarpium Granati (Pomegranate Peel) is the dried peel of Punica granatum L., and the acid of its property, taste are sent out puckery, have hemostasis, astringing intestine to stop diarrhea, parasiticidal effectiveness, are clinical conventional Chinese medicines.Pomegranate peel polyphenol is one type of general name with polyphenol hydroxyl chemical compound; Comprise multiple chemical compounds such as Galla Turcica (Galla Helepensis) tannin, elladitannin, ellagic acid, chlorogenic acid, gallic acid, catechin, epicatechin, anthocyanidin, ferulic acid and quercetin; Mainly be present in the Pericarpium Granati, content is about 10 ~ 20% of its dry weight.
In recent years, big quantity research shows, pomegranate peel polyphenol has free radical resisting, antimutagenic, aging resistance, antibacterial, angiocardiopathy preventing, improve multiple function such as climacteric syndrome, and effect is widely all arranged in fields such as medicine, article of everyday use and food.
In recent years; Have what pomegranate peel polyphenol was studied and applied for a patent: Wang Chaoping etc. (number of patent application CN201010613907) disclose a kind of method of from Pericarpium Granati, extracting 2-acetonylpiperidine; Enzyme is closed ultrasonic broken wall treatment close ultrasonic lixiviate with alcohol and effectively combine, from Pericarpium Granati, extract 2-acetonylpiperidine.Guo Changjiang etc. (number of patent application CN200810153555) disclose a kind of method for distilling of fresh pomegranate bark antioxidants, fresh pomegranate are immersed in the mixed solvent, and soaking and extracting 0.1～2 hour, concentrating under reduced pressure is dry, obtains pomegranate bark antioxidants.Its external to superoxide anion, hydroxy radical, lipid free radical the removing ability and the protective effect of LDL oxidation all is better than the pomegranate fruit extract.Yang Mingfu etc. (number of patent application CN 200810306391) disclose a kind of method of extracting Punicalagin and ellagic acid simultaneously from Pericarpium Granati.Pericarpium Granati is earlier with 25～35% ethanol extractions, and extracting solution concentrates the back and adsorbs with macroporous resin, and concentrate eluant promptly gets the Punicalagin extract after the desorption; The Pericarpium Granati that will extract Punicalagin again is with 70～90% ethanol percolate extraction, and adding NaOH was hydrolyzed after extracting solution concentrated and removes alcohol, added HCl after the hydrolysis, produced deposition, separated, and gained precipitates and is the ellagic acid extract.
Above-mentioned method for preparing many with organic solvent as extracting medium, Shang Weijian is the relevant report of extracting medium with water.
Summary of the invention
The technical problem that the present invention solved provides a kind of brand-new method from Pericarpium Granati extraction pomegranate peel polyphenol, and this method has advantage easy and simple to handle, that extraction efficiency is high.
The present invention is characterized in that from the method for Pericarpium Granati extraction pomegranate peel polyphenol: be solvent with water, adopt microwave extraction to obtain the pomegranate peel polyphenol crude extract, separate through macroporous resin then, obtain pomegranate peel polyphenol behind concentrating under reduced pressure, the vacuum freeze-drying.Wherein, total polyphenols content 30-55% in the gained pomegranate peel polyphenol crude extract; Total polyphenols content is 65-80% in the gained pomegranate peel polyphenol.
Concrete, the present invention comprises the steps: from the method for Pericarpium Granati extraction pomegranate peel polyphenol
A, pulverizing Pericarpium Granati; Preferably, Pericarpium Granati is crushed to>40 orders, further preferred powder is broken to the 80-100 order;
B, extraction: solvent is a water, adopts microwave extraction, and dry extraction liquid gets the pomegranate peel polyphenol crude extract; Wherein, the microwave extraction condition is: microwave power 200-800W, temperature 60-95 ℃, liquid material be than 15:1-40:1, extraction time 60-180S; Preferred microwave extraction condition is: 75 ℃ of temperature, liquid material be than 30:1, extraction time 140S;
C, purification: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 4-12mg/mL, crosses D101, D140 or AB-8 macroporous resin, and speed is 0.5-5.5BV/h; Adopt consumption to clean greater than the water of 5BV; The employing consumption is 1-6.5BV, the concentration ethanol elution greater than 60%v/v/; Collect eluent, drying gets pomegranate peel polyphenol;
Further, preferred purification condition is: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 10mg/mL; The macroporous resin resin adopts the D101 macroporous resin; The speed that the solution that the pomegranate peel polyphenol crude extract adds water preparation is crossed macroporous resin is 2BV/h; Adopt the water of consumption 5BV to clean; Adopt the ethanol of concentration greater than 70%v/v/, consumption is the 5.5BV eluting.
To sum up; Method for distilling of the present invention is a solvent with water, utilizes microwave from Pericarpium Granati, can effectively extract pomegranate peel polyphenol, and total polyphenols content is higher in gained pomegranate peel polyphenol crude extract and the pomegranate peel polyphenol; The crude extract yield reaches 40-50%, for obtaining pomegranate peel polyphenol a kind of new selection is provided.
Description of drawings
Each solvent total polyphenols extraction ratio of Fig. 1
Fig. 2 pomegranate peel polyphenol concentration is selected
Fig. 3 purification eluant strength is selected
The dynamic adsorption of Fig. 4 polyphenol
The selection of Fig. 5 eluent consumption
The specific embodiment
Below through to the description of specific embodiment of the invention explanation but do not limit the present invention.
The present invention is characterized in that from the method for Pericarpium Granati extraction pomegranate peel polyphenol: be solvent with water, adopt microwave extraction to obtain the pomegranate peel polyphenol crude extract, separate through macroporous resin then, obtain pomegranate peel polyphenol behind concentrating under reduced pressure, the vacuum freeze-drying.
One, the selection of extraction step:
1, the inventor once attempted with existing water boiling and extraction method: Pericarpium Granati is used water boiling and extraction; 100 ℃, solid-liquid ratio are 1:30, extract 15min; Extract 2 times, extracting solution is thickness relatively, and is difficult to carry out sucking filtration; Soluble protein, pectin and soluble saccharide content are more in the extracting solution, and total polyphenols content is 13.18g/100g.This water extract is to DPPH, OH, O
2 -Partly remove concentration and suppress the MDA 503nhibiting concentration and be respectively: 28.46mg/L, 79.31mg/L, 375.69mg/L, 60.56mg/L.The mode that adopts Direct Water to carry, in view of its impurity content is high, its oxidation resistance is also relatively low.
2, thereafter, the inventor has also attempted following three kinds of method for distilling.
Ultrasonic-assisted extraction: 40% alcoholic solution, the solid-liquid ratio of 1:30,60 ℃ extract 30 minutes, extract 3 times, the ultrasonic operating frequency of 36kHz, concentrate, lyophilization, the total polyphenols extraction ratio is 25.86g/100g.
Heat reflow method: concentration of alcohol is 70%, extract temperature is that 89 ℃, solid-liquid ratio are 1:26, extract 2.5h, extract 2 this, concentrate, lyophilization, the total polyphenols extraction ratio is 21.46g/100g.
The microwave-assisted extraction method: extracting temperature is 75 ℃, and extraction time is 140S, and solid-liquid ratio is 1:30, is to extract solvent with water, extract 2 times, and concentrated, lyophilization, the total polyphenols extraction ratio is 22.06g/100g.
This shows the microwave-assisted extraction method, is to extract solvent with water, does not poison, and cost is low, save time, and extraction ratio is suitable with ultrasonic-assisted extraction and the heat reflow method used always.
3, extract the screening of solvent
Getting Pericarpium Granati 10g, 80-100 order, 70 ℃ of temperature, solid-liquid ratio is 1:30, microwave-assisted extraction time 2min, uses methanol, ethanol, water to carry out lixiviate as solvent respectively, and the result sees Fig. 1.Wherein, the extraction ratio of methanol is 20.32%, alcoholic acid extraction ratio is 19.22%, the extraction ratio of water is 19.39%, and extraction ratio is all more approaching, considers from economic angle with safety, so experimental selection water is solvent.
Through above-mentioned 3 investigation, confirm that finally extraction step of the present invention is the microwave-assisted extraction method: be solvent with water, adopt microwave extraction, dry extraction liquid gets the pomegranate peel polyphenol crude extract; The microwave extraction condition is: microwave power 200-800W, temperature 60-95 ℃, liquid material be than 15:1-40:1, extraction time 60-180S.Preferred microwave extraction condition is: 75 ℃ of temperature, liquid material be than 30:1, extraction time 140S.
Two, the selection of purification step
1, the selection of macroporous resin
The microwave-assisted extraction method that Pericarpium Granati adopts extraction step optimization to obtain obtains the pomegranate peel polyphenol crude extract, adds the solution that water is mixed with 9mg/mL then, adopts in the table 1 after all types of macroporous resin adsorption.Measure the polyphenol concentration Pe (mg/mL) of supernatant then, follow solution in the elimination triangular flask, distilled water wash twice adds dehydrated alcohol the same terms then and handled 3 hours, surveys the polyphenol concentration Pd (mg/mL) of its stripping liquid.Calculate the polyphenol mass fraction of adsorption rate, adsorbance, desorption efficiency, the response rate and desorbing thing respectively by following formula.
Adsorbance (mg/g dried resin)=(Po-Pe) * Vo/m
Adsorption rate (%)=(Po-Pe) * 100/Po
Desorption efficiency (%)=Pd*100/ (Po-Pe)
The response rate (%)=Pd*100/Po
Desorbing thing polyphenol mass fraction (%)=Pd*Vd * 100/Md
More than various in: Po is the mass concentration (mg/mL) of polyphenol in the Pericarpium Granati extract, and Vo is Pericarpium Granati extract liquor capacity (mL), and Vd is stripping liquid volume (mL), and M is resin quality (g), and Md is the quality (mg) of solid content in the stripping liquid.
The result: each macroporous resin is seen table 1 to the static saturated adsorption and desorption parameter of pomegranate peel polyphenol, and the result shows: D101, D140 or AB-8 adsorption rate maximum and desorption efficiency are higher relatively, can be used as the used macroporous resin of purification step of the present invention.And preferably adopt the D101 resin.
Each macroporous resin of table 1 is to the static saturated adsorption and desorption parameter of pomegranate peel polyphenol
2, pomegranate peel polyphenol concentration is selected
Investigate the influence of the difference of pomegranate peel polyphenol concentration to its resin absorption effect, experimental result is seen Fig. 2.
Can know from Fig. 2, constantly strengthen with pomegranate peel polyphenol concentration, resin be adsorbed with downward trend, the difference of 4-10mg/mL is little, and has than big-difference greater than 10mg/mL, the consideration when economizing on, the concentration of selecting 10mg/mL here is upper prop concentration.
3, the eluant concentration of ethanol is selected
Investigate ethanol elution agent variable concentrations to the influence of resin to pomegranate peel polyphenol absorption, experimental result Fig. 3.
Can know from Fig. 3; The concentration change of release agent is bigger to the influence of D101 resin absorption pomegranate peel polyphenol, strengthens with the concentration of resolving agent, and desorption efficiency also has the trend of rising; After the alcohol desorption agent concentration reaches 70%v/v; Resolution factor tends to be steady, thus can select concentration to get final product greater than 60%v/v, and consider to select the concentration ratio of 70%v/v more suitable from the angle of economy.
4, macroporous resin is to the dynamic adsorption and the desorption experiment of pomegranate peel polyphenol
1) macroporous resin is to the polyphenol dynamic adsorption
Through the mensuration of different phase effluent, draw the dynamic adsorption curve of resin, experimental result Fig. 4.
Can be known that by Fig. 4 with the increase of advancing the post amount, the polyphenol content of effluent is also increasing, this shows that into the post amount is big more, and the adsorption effect of resin is poor more, and this is because the approaching equilibrated cause of the absorption of resin.Under this kind condition, when advancing the post amount greater than 4BV, polyphenol content stops to strengthen in the effluent, and this is that the absorption that proves resin has reached balance.
2) resin water for cleaning amount experimental result
There is certain polysaccharide to exist in the Pericarpium Granati extract; Can take macroporous resin that it and the absorption difference of pomegranate peel polyphenol are separated both; After Pericarpium Granati extract solution advanced post by certain condition, wait resin fully to adsorb, water cleans resin; Unnecessary polysaccharide is removed, and the polysaccharide reaction is seen table 2 with the polyphenol Loss Rate.
The reaction test of table 2 polysaccharide
Annotate :+be the positive reaction of polysaccharide;-be the negative reaction of polysaccharide
Can learn by table 2, when eluting water during greater than 5BV, the polysaccharide reaction that is negative; Show like this and removed a large amount of impurity, but with the increasing of the eluting water yield, wherein the turnover rate of polyphenol is also constantly increasing; So take all factors into consideration, the rinse water consumption with 5BV is selected in final decision.
3) ethanol elution agent consumption is confirmed
Macroporous resin is seen Fig. 5 to pomegranate peel polyphenol desorption experiment curve.
Can learn by Fig. 5; When the consumption of strippant is 5.5BV; The desorbing degree of pomegranate peel polyphenol reaches capacity basically, and polyphenol content is 0.5mg/mL in ethanolysis imbibition this moment, and most polyphenol escapes during for 5BV at the release agent consumption; Consider efficient and economic factor, selecting ethanol release agent consumption here is 5.5BV.
5, the mensuration result behind the pomegranate peel polyphenol purification
Pomegranate peel polyphenol after the D101 type of learning from else's experience purification by macroporous resin is handled is mixed with the solution that concentration is 0.1mg/mL with it, and blank pipe is distilled water; With its at absorbance 283nm place measured value, parallel do 5 this, bring the polyphenol regression equation into; Calculate its result, data are shown in table 3 table.
Table 3 polyphenol purification thing assay
Can find out from top experimental result; Pomegranate peel polyphenol content behind the purification of macroporous resin can reach 71.64% ± 0.56 (RSD=0.78%); Than before not during purification 49.31% pomegranate peel polyphenol content increased by 22.33%; This explanation the method has certain purification effect, can be used for the purifies and separates of pomegranate peel polyphenol.
Through above-mentioned 5 investigation, confirm that finally purification step of the present invention is: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 4-12mg/mL, crosses D101, D140 or AB-8 macroporous resin, and speed is 0.5-5.5BV/h; Water for cleaning is 1-6BV, and concentration is greater than the ethanol elution agent of 60%v/v/, and use amount is 1-6.5BV, and collection, drying get pomegranate peel polyphenol.Preferred purification condition is: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 10mg/mL, crosses the D101 macroporous resin, and speed is 2BV/h; Water for cleaning is 5BV, 70% ethanol elution agent, and use amount is 5.5BV, collection, drying get pomegranate peel polyphenol.
Below, foregoing of the present invention is remake further detailed description through the specific embodiment of embodiment form.
1, pulverizes, sieves
Pericarpium Granati (buying from Chengdu Chinese medicine beverage company limited), the 80-100 order is sieved in oven dry, pulverizing.
2, thick polyphenol extracts
Adopt microwave extracting appearance (Xinyi Microwave Chemistry Tech Co., Ltd.) to carry out assisted extraction; Optimizing extracting parameter is: extract 75 ℃ of temperature, liquid material than 30:1, extraction time 140S; Extract polyphenols with 80-100 purpose Punica granatum L. PIFEN; Total polyphenols content is 22.06% in the crude extract, and behind concentrating under reduced pressure and vacuum freeze-drying, total polyphenols content is 49.31%.
3, separation, purification
Adopt the D101 macroporous resin, speed is 2BV/h, and advancing post concentration is 10mg/mL, and water for cleaning is 5BV; 70% ethanol elution agent, use amount is 5.5BV, obtains pomegranate peel polyphenol; Behind concentrating under reduced pressure and vacuum freeze-drying, get pomegranate peel polyphenol, total polyphenols content is 71.64%.
Main active is Punicalagin, ellagic acid, chlorogenic acid, epicatechin, Quercetin in the gained pomegranate peel polyphenol of the present invention, adopts RC-HPLC to analyze.
The Punicalagin chromatographic condition: chromatographic column is Hypersil ODS2 (250nm * 4.6nm; 5 μ m Dalian are according to Lyntech Corporation (US) 10177 South 77th East Avenue Tulsa, Oklahoma 74133 U.S.), mobile phase is methanol-2% glacial acetic acid (7:93), volume flow is 1.0mL/min; The detection wavelength is 232nm; 25 ℃ of column temperatures, greater than 8000, separating degree is greater than 1.5 by ellagic acid for theoretical cam curve.
Ellagic acid, chlorogenic acid, epicatechin and Quercetin mix the mark chromatographic condition: chromatographic column is Hypersil ODS2 (250nm * 4.6nm; 5 μ m Dalian are according to Lyntech Corporation (US) 10177 South 77th East Avenue Tulsa, Oklahoma 74133 U.S.); Mobile phase is acetonitrile-0.4% phosphoric acid (17:83); Flow velocity is 1.0mL/min, detects wavelength 310nm, and column temperature is 30 ℃; Greater than 8000, the separating degree of ellagic acid (retention time is 8.681min), Quercetin (retention time is 17.238min), chlorogenic acid (retention time is 4.392min), epicatechin (retention time is 5.463min) is greater than 1.5 by ellagic acid for theoretical cam curve.
Analyze through RC-HPLC, in the embodiment 1 gained pomegranate peel polyphenol, Punicalagin, ellagic acid, chlorogenic acid, epicatechin, Quercetin are respectively: 46.91%, 9.44%, 0.53%, 0.32%, 0.75%.
DPPH, OH, O
2 -Assay method with MDA:
1, the removing of DPPH is active
Accurately take by weighing DPPH standard substance 20mg, dehydrated alcohol is settled to 500mL, and getting concentration is 0.04mg/mL solution.Get this solution 3mL, add the Pericarpium Granati extracting solution 1mL of variable concentrations, room temperature 30min measures absorbance, and blank control group replaces sample with the 1mL dehydrated alcohol, and is calculated as follows clearance rate:
DPPH clearance rate=(1-A
Sample/ A
Blank) * 100%
2, the removing of OH is active
Adopt the ferrous ion catalyzing hydrogen peroxide to produce hydroxy radical (Fonton reaction) method.
Get 0.75mmol/L orthophenanthroline solution 1mL, PBS liquid 2mL and distilled water 1mL fully behind the mixing, add 0.75mmol/L ferrous sulfate 1mL, and mixing adds 0.01% hydrogen peroxide 1mL, 37 ℃ of incubation 60min, and the 536nm place surveys its absorbance, and its value is A
PReplace the 1mL hydrogen peroxide with the 1mL distilled water, recording absorbance is A
BReplace the 1mL distilled water with sample 1mL, recording absorbance is A
SOH clearance rate (d%) computing formula:
3, O
2 -Removing active
Get 0.05mol/L, the Tris-HCl buffer 4.5mL of pH8.2 places 25 ℃ of water-bath preheating 20min in test tube; Add the Pericarpium Granati extracting solution 0.1mL of variable concentrations, 2.5mol/L pyrogallol 0.4mL reacts 4min behind the mixing in 25 ℃ of water-baths; Use two cessation reactions of 8mol/L HCl immediately; And measure absorbance (returning to zero) with distilled water at 299 ± 1nm place, the 0.1mL distilled water of blank control group replaces sample, and is calculated as follows clearance rate:
Clearance rate=(1-A
Sample/ A
Blank] * 100%
4, lipid peroxidation inhibitory action
Mice is got hepatic tissue after putting to death, and cleans blood stains, shreds with 3% homogenate of normal saline system.Get this homogenate 0.3mL, add people's different quality concentration sample 0.17mL, 0.0024% hydrogen peroxide 0.1mL; 0.03mmol/L ferrous sulfate 0.1mL, 37 ℃ of insulation 60min add people 1mL20% trichloroacetic acid and 1mL0.67%TBA; Water-cooled behind 90 ℃ of insulation 15min, the centrifugal 20min of 4000r/min gets supernatant and measures the A value at the 532nm place; It is blank pipe that distilled water replaces supplying the reaction tube of test agent, and lipid peroxidation suppression ratio E is:
E=(1-A
Sample/ A
Blank) * 100%
Result: embodiment 1 gained pomegranate peel polyphenol is to DPPH, OH, O
2 -Be respectively with the MDA 503nhibiting concentration: 26.92mg/mL, 49.88mg/mL, 482.45mg/mL, 3.13mg/mL.
Antioxidant effect with reference to embodiment 3 detection methods contrasts pomegranate peel polyphenol and gallic acid, green tea polyphenol.
The result is following:
Pomegranate peel polyphenol, gallic acid and green tea polyphenol are partly removed concentration to what DPPH removed, are respectively 26.92mg/L, 37.65mg/L, 22.48mg/L.
Pomegranate peel polyphenol, gallic acid and green tea polyphenol are partly removed concentration to what OH removed, are respectively 49.88mg/L, 51.85mg/L, 66.67mg/L.
Pomegranate peel polyphenol, gallic acid and green tea polyphenol are to O
2 -That removes partly removes concentration, is respectively 482mg/L, 289.20mg/L, 1043.91mg/L.
The 503nhibiting concentration that pomegranate peel polyphenol, gallic acid and green tea polyphenol are removed MDA is respectively 3.13mg/L, 22.82mg/L, 3.47mg/L.
The result shows: gained pomegranate peel polyphenol of the present invention is higher than gallic acid and approaching with green tea polyphenol in the removing activity of DPPH.In the removing to OH, the pomegranate peel polyphenol activity is higher than gallic acid and green tea polyphenol.To O
2 -Scavenging action in, the removing activity of pomegranate peel polyphenol is higher than green tea polyphenol.In the inhibitory action to lipid peroxidation, pomegranate peel polyphenol is higher than the inhibition activity of gallic acid and green tea polyphenol.Because gained pomegranate peel polyphenol of the present invention has very high antioxidant activity, can be made into health product, medicine and daily cosmetics with anti-oxidation function.
Claims (10)
1. extract the method for pomegranate peel polyphenol from Pericarpium Granati, it is characterized in that: be solvent with water, adopt microwave extraction to obtain the pomegranate peel polyphenol crude extract, separate through macroporous resin then, obtain pomegranate peel polyphenol behind concentrating under reduced pressure, the vacuum freeze-drying.
2. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 1 is characterized in that:
A, pulverizing Pericarpium Granati;
B, extraction: solvent is a water, adopts microwave extraction, and dry extraction liquid gets the pomegranate peel polyphenol crude extract;
Wherein, the microwave extraction condition is: temperature 60-95 ℃, liquid material be than 15:1-40:1, extraction time 60-180S;
C, purification: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 4-12mg/mL, crosses D101, D140 or AB-8 macroporous resin, and speed is 0.5-5.5BV/h; Adopt consumption to clean greater than the water of 5BV; The employing consumption is 1-6.5BV, the concentration ethanol elution greater than 60%v/v/; Collect eluent, drying gets pomegranate peel polyphenol.
3. according to claim 2ly extract the method for pomegranate peel polyphenol from Pericarpium Granati, it is characterized in that: the preferred microwave extraction condition of step B is: 75 ℃ of temperature, liquid material be than 30:1, extraction time 140S.
4. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 2, it is characterized in that: in the step C purification condition: the pomegranate peel polyphenol crude extract adds the solution that water is mixed with 10mg/mL.
5. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 2, it is characterized in that: in the step C purification condition: the macroporous resin resin adopts the D101 macroporous resin.
6. according to claim 2ly extract the method for pomegranate peel polyphenol from Pericarpium Granati, it is characterized in that: in the step C purification condition: the speed that the solution that the pomegranate peel polyphenol crude extract adds the water preparation is crossed macroporous resin is 2BV/h.
7. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 2 is characterized in that: in the step C purification condition: adopt the water of consumption 5BV to clean.
8. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 2 is characterized in that: in the step C purification condition: adopt the ethanol of concentration greater than 70%v/v/, consumption is the 5.5BV eluting.
9. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 2 is characterized in that: steps A is pulverized the Pericarpium Granati granularity extremely>40 orders.
10. the method from Pericarpium Granati extraction pomegranate peel polyphenol according to claim 1 is characterized in that: steps A is pulverized the Pericarpium Granati granularity to the 80-100 order.
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| CN114466648A (en) * | 2019-10-01 | 2022-05-10 | 莱雅公司 | Pomegranate extracts and cosmetic use thereof |
| CN112028798A (en) * | 2020-09-17 | 2020-12-04 | 永州职业技术学院 | Garlic microwave high-fire enzyme deactivation device for garlic alliin extraction and use method thereof |
| CN113717139A (en) * | 2021-09-15 | 2021-11-30 | 耒阳市刘燕酿制生物科技有限公司 | Method for extracting quercetin from pomegranate peel |
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| CN115606734B (en) * | 2022-09-15 | 2024-04-30 | 滇西科技师范学院 | Antioxidant extract of elaeagnus conferta roxb as well as preparation method and application thereof |
| CN115918819A (en) * | 2022-12-02 | 2023-04-07 | 广东省农业科学院蚕业与农产品加工研究所 | Pretreatment method for processing soft-seed pomegranate |
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