CN105308185A - 使用羧基官能化超顺磁性纳米团簇的磁性分离方法 - Google Patents
使用羧基官能化超顺磁性纳米团簇的磁性分离方法 Download PDFInfo
- Publication number
- CN105308185A CN105308185A CN201480033159.1A CN201480033159A CN105308185A CN 105308185 A CN105308185 A CN 105308185A CN 201480033159 A CN201480033159 A CN 201480033159A CN 105308185 A CN105308185 A CN 105308185A
- Authority
- CN
- China
- Prior art keywords
- super
- nano cluster
- paramagnetism nano
- paramagnetism
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 137
- 238000007885 magnetic separation Methods 0.000 title claims description 7
- 230000008569 process Effects 0.000 title abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 81
- 244000005700 microbiome Species 0.000 claims abstract description 79
- 230000005291 magnetic effect Effects 0.000 claims description 58
- 239000000463 material Substances 0.000 claims description 47
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 38
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 36
- 230000015572 biosynthetic process Effects 0.000 claims description 27
- 125000000524 functional group Chemical group 0.000 claims description 27
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 238000003786 synthesis reaction Methods 0.000 claims description 24
- 239000002105 nanoparticle Substances 0.000 claims description 23
- 230000009871 nonspecific binding Effects 0.000 claims description 22
- 239000002861 polymer material Substances 0.000 claims description 22
- 238000012360 testing method Methods 0.000 claims description 19
- 241000894006 Bacteria Species 0.000 claims description 18
- 239000011248 coating agent Substances 0.000 claims description 18
- 238000000576 coating method Methods 0.000 claims description 18
- 235000013305 food Nutrition 0.000 claims description 16
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 13
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 238000004458 analytical method Methods 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 12
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 8
- 241000588724 Escherichia coli Species 0.000 claims description 8
- 239000000178 monomer Substances 0.000 claims description 8
- PQUXFUBNSYCQAL-UHFFFAOYSA-N 1-(2,3-difluorophenyl)ethanone Chemical compound CC(=O)C1=CC=CC(F)=C1F PQUXFUBNSYCQAL-UHFFFAOYSA-N 0.000 claims description 7
- 230000000968 intestinal effect Effects 0.000 claims description 7
- 229920000620 organic polymer Polymers 0.000 claims description 7
- 229940047670 sodium acrylate Drugs 0.000 claims description 7
- 239000007795 chemical reaction product Substances 0.000 claims description 6
- 239000011541 reaction mixture Substances 0.000 claims description 6
- 230000001900 immune effect Effects 0.000 claims description 4
- 238000001027 hydrothermal synthesis Methods 0.000 claims description 3
- 238000000386 microscopy Methods 0.000 claims description 3
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- 239000008247 solid mixture Substances 0.000 claims description 3
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- GAGFBARWNSCQEP-UHFFFAOYSA-N CC(O)=O.CC(O)=O.CC(O)=O.COC([SiH3])(OC)CC(OC)NCCN Chemical compound CC(O)=O.CC(O)=O.CC(O)=O.COC([SiH3])(OC)CC(OC)NCCN GAGFBARWNSCQEP-UHFFFAOYSA-N 0.000 claims description 2
- 241000186779 Listeria monocytogenes Species 0.000 claims description 2
- 238000003149 assay kit Methods 0.000 claims description 2
- 229940115931 listeria monocytogenes Drugs 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 145
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 28
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 23
- 230000000052 comparative effect Effects 0.000 description 19
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 16
- 239000008367 deionised water Substances 0.000 description 16
- 229910021641 deionized water Inorganic materials 0.000 description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- -1 trimethoxy-silylpropyl Chemical group 0.000 description 15
- 239000004743 Polypropylene Substances 0.000 description 12
- DZRJLJPPUJADOO-UHFFFAOYSA-N chaetomin Natural products CN1C(=O)C2(Cc3cn(C)c4ccccc34)SSC1(CO)C(=O)N2C56CC78SSC(CO)(N(C)C7=O)C(=O)N8C5Nc9ccccc69 DZRJLJPPUJADOO-UHFFFAOYSA-N 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 12
- 239000002245 particle Substances 0.000 description 12
- 229920001155 polypropylene Polymers 0.000 description 12
- 239000000377 silicon dioxide Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 239000012530 fluid Substances 0.000 description 10
- 238000007747 plating Methods 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 235000015278 beef Nutrition 0.000 description 9
- DIUIQJFZKRAGBZ-UHFFFAOYSA-N chetoseminudin A Natural products O=C1C(SSS2)(CO)N(C)C(=O)C32CC2(N4C5=CC=CC=C5C(CC56C(N(C)C(CO)(SS5)C(=O)N6C)=O)=C4)C4=CC=CC=C4NC2N31 DIUIQJFZKRAGBZ-UHFFFAOYSA-N 0.000 description 9
- 238000002360 preparation method Methods 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- 230000000813 microbial effect Effects 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 229920002125 Sokalan® Polymers 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 235000015220 hamburgers Nutrition 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000005336 cracking Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- NQXWGWZJXJUMQB-UHFFFAOYSA-K iron trichloride hexahydrate Chemical compound O.O.O.O.O.O.[Cl-].Cl[Fe+]Cl NQXWGWZJXJUMQB-UHFFFAOYSA-K 0.000 description 5
- 239000006249 magnetic particle Substances 0.000 description 5
- 239000004584 polyacrylic acid Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 229960001866 silicon dioxide Drugs 0.000 description 5
- 235000012239 silicon dioxide Nutrition 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- 241000607768 Shigella Species 0.000 description 4
- 239000006087 Silane Coupling Agent Substances 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 239000011258 core-shell material Substances 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000009940 knitting Methods 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 3
- 241000186781 Listeria Species 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 241000607142 Salmonella Species 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- BCKXLBQYZLBQEK-KVVVOXFISA-M Sodium oleate Chemical compound [Na+].CCCCCCCC\C=C/CCCCCCCC([O-])=O BCKXLBQYZLBQEK-KVVVOXFISA-M 0.000 description 3
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 description 3
- 241000607734 Yersinia <bacteria> Species 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000007834 ligase chain reaction Methods 0.000 description 3
- 230000002101 lytic effect Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 239000011164 primary particle Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- SCPYDCQAZCOKTP-UHFFFAOYSA-N silanol Chemical compound [SiH3]O SCPYDCQAZCOKTP-UHFFFAOYSA-N 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 2
- 241000193738 Bacillus anthracis Species 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 241000588807 Bordetella Species 0.000 description 2
- 241000589968 Borrelia Species 0.000 description 2
- 241000589876 Campylobacter Species 0.000 description 2
- 241000222122 Candida albicans Species 0.000 description 2
- 241000193163 Clostridioides difficile Species 0.000 description 2
- 241000193155 Clostridium botulinum Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000194033 Enterococcus Species 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 208000016604 Lyme disease Diseases 0.000 description 2
- 241000186359 Mycobacterium Species 0.000 description 2
- 241000588653 Neisseria Species 0.000 description 2
- 241000191940 Staphylococcus Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 241000607598 Vibrio Species 0.000 description 2
- 241000607626 Vibrio cholerae Species 0.000 description 2
- 241000607272 Vibrio parahaemolyticus Species 0.000 description 2
- 241000607265 Vibrio vulnificus Species 0.000 description 2
- 241000607447 Yersinia enterocolitica Species 0.000 description 2
- 229940048053 acrylate Drugs 0.000 description 2
- 150000001408 amides Chemical class 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000005137 deposition process Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003302 ferromagnetic material Substances 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000012252 genetic analysis Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000005389 magnetism Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000005408 paramagnetism Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 239000002002 slurry Substances 0.000 description 2
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical class [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000001308 synthesis method Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 229940118696 vibrio cholerae Drugs 0.000 description 2
- 229940098232 yersinia enterocolitica Drugs 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- LDHQCZJRKDOVOX-UHFFFAOYSA-N 2-butenoic acid Chemical compound CC=CC(O)=O LDHQCZJRKDOVOX-UHFFFAOYSA-N 0.000 description 1
- CVOFKRWYWCSDMA-UHFFFAOYSA-N 2-chloro-n-(2,6-diethylphenyl)-n-(methoxymethyl)acetamide;2,6-dinitro-n,n-dipropyl-4-(trifluoromethyl)aniline Chemical compound CCC1=CC=CC(CC)=C1N(COC)C(=O)CCl.CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O CVOFKRWYWCSDMA-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- GFDKDHGFNSWQRU-UHFFFAOYSA-N C(C=C)(=O)O.C[Na] Chemical compound C(C=C)(=O)O.C[Na] GFDKDHGFNSWQRU-UHFFFAOYSA-N 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241001333951 Escherichia coli O157 Species 0.000 description 1
- JOYRKODLDBILNP-UHFFFAOYSA-N Ethyl urethane Chemical compound CCOC(N)=O JOYRKODLDBILNP-UHFFFAOYSA-N 0.000 description 1
- 239000012692 Fe precursor Substances 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000605909 Fusobacterium Species 0.000 description 1
- 241000589989 Helicobacter Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical group [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 244000285963 Kluyveromyces fragilis Species 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241001138501 Salmonella enterica Species 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical group [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 241000607477 Yersinia pseudotuberculosis Species 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000000908 ammonium hydroxide Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 229940075612 bacillus cereus Drugs 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000009583 bone marrow aspiration Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 229940038704 clostridium perfringens Drugs 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000007821 culture assay Methods 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 230000005595 deprotonation Effects 0.000 description 1
- 238000010537 deprotonation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000009881 electrostatic interaction Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940023064 escherichia coli Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- OUDSFQBUEBFSPS-UHFFFAOYSA-N ethylenediaminetriacetic acid Chemical compound OC(=O)CNCCN(CC(O)=O)CC(O)=O OUDSFQBUEBFSPS-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229940032296 ferric chloride Drugs 0.000 description 1
- 229940044631 ferric chloride hexahydrate Drugs 0.000 description 1
- 239000002902 ferrimagnetic material Substances 0.000 description 1
- 239000011554 ferrofluid Substances 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 244000078673 foodborn pathogen Species 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000012456 homogeneous solution Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 150000003949 imides Chemical class 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 238000001725 laser pyrolysis Methods 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N lysine Chemical compound NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 108010056929 lyticase Proteins 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 125000002524 organometallic group Chemical group 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000002907 paramagnetic material Substances 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 1
- 238000006068 polycondensation reaction Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000012056 semi-solid material Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000007614 solvation Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000004154 testing of material Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- FRGBDYDAIWEXJX-UHFFFAOYSA-K trisodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(3-trimethoxysilylpropyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].CO[Si](OC)(OC)CCCN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O FRGBDYDAIWEXJX-UHFFFAOYSA-K 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
- G01N15/0612—Optical scan of the deposits
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/019—Biological contaminants; Fouling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361833545P | 2013-06-11 | 2013-06-11 | |
| US61/833,545 | 2013-06-11 | ||
| PCT/US2014/041016 WO2014200790A1 (en) | 2013-06-11 | 2014-06-05 | Magnetic separation process using carboxyl-functionalized superparamagnetic nanoclusters |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105308185A true CN105308185A (zh) | 2016-02-03 |
Family
ID=52022669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201480033159.1A Pending CN105308185A (zh) | 2013-06-11 | 2014-06-05 | 使用羧基官能化超顺磁性纳米团簇的磁性分离方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20160122797A1 (enExample) |
| EP (1) | EP3008203A4 (enExample) |
| JP (1) | JP2016526378A (enExample) |
| CN (1) | CN105308185A (enExample) |
| WO (1) | WO2014200790A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954268A (zh) * | 2016-04-22 | 2016-09-21 | 华南师范大学 | 一种基于四氧化三铁纳米簇催化信号放大的可视化检测传感器及其构建与应用 |
| CN108496231A (zh) * | 2016-08-19 | 2018-09-04 | 阿莫生命科学有限公司 | 制备超顺磁性纳米复合材料的方法及使用该方法制备的超顺磁性纳米复合材料 |
| CN110501208A (zh) * | 2018-05-17 | 2019-11-26 | 国家纳米科学中心 | 叶酸功能化的链霉亲和素修饰的磁性纳米颗粒、其制备方法和应用 |
| CN110548489A (zh) * | 2019-09-12 | 2019-12-10 | 安徽工业大学 | 一种氨基磁性纳米粒子的制备方法及其在dna提取中的应用 |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015166415A1 (en) * | 2014-04-28 | 2015-11-05 | Universidade De Aveiro | Chelator modified magnetic silica nanoparticles, their use and preparation |
| CN107073161B (zh) | 2014-10-01 | 2020-11-03 | 3M创新有限公司 | 用于清创术的多孔装置、套件和方法 |
| FR3071609B1 (fr) * | 2017-09-27 | 2019-10-04 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Procede de detection de microorganismes dans un echantillon |
| KR102008622B1 (ko) * | 2018-09-18 | 2019-08-07 | (주)바이오액츠 | 생체물질 도입을 위한 링커 및 상기 링커가 결합된 자성 나노입자 |
| KR20240099505A (ko) * | 2022-04-18 | 2024-06-28 | 엔. 잉글리쉬 | 용매화된 나노클러스터를 제조하는 방법 및 생성기 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432780A (zh) * | 2011-09-16 | 2012-05-02 | 复旦大学 | 一种表面羧基功能化的核壳式磁性复合微球及其制备方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7795041B2 (en) * | 2004-02-11 | 2010-09-14 | Massachusetts Institute Of Technology | Multi-polymer-coated magnetic nanoclusters |
| WO2008123844A2 (en) * | 2006-02-08 | 2008-10-16 | University Of Toledo | System for detecting nanoparticles using modulated surface plasmon resonance (mspr) |
| EP2205717B1 (en) * | 2007-10-03 | 2017-08-16 | 3M Innovative Properties Company | Microorganism concentration process |
| BRPI0817602B1 (pt) * | 2007-10-03 | 2018-01-16 | 3M Innovative Properties Company | Processo para capturar ou concentrar micro- organismos e kits de diagnóstico |
| BRPI0817415B8 (pt) * | 2007-10-03 | 2021-07-27 | 3M Innovative Properties Co | processo e kit para capturar ou concentrar microorganismos |
| EP2414503B1 (en) * | 2009-04-03 | 2014-04-23 | 3M Innovative Properties Company | Microorganism concentration process and device |
| CN102686995A (zh) * | 2009-12-22 | 2012-09-19 | 3M创新有限公司 | 微生物浓集方法以及用于其中的浓集剂 |
| JP5957437B2 (ja) * | 2010-03-24 | 2016-07-27 | ヴァイヴ クロップ プロテクション インコーポレイテッドＶｉｖｅ Ｃｒｏｐ Ｐｒｏｔｅｃｔｉｏｎ Ｉｎｃ． | 中性有機化合物とポリマーナノ粒子とを配合する方法 |
-
2014
- 2014-06-05 CN CN201480033159.1A patent/CN105308185A/zh active Pending
- 2014-06-05 WO PCT/US2014/041016 patent/WO2014200790A1/en not_active Ceased
- 2014-06-05 JP JP2016519547A patent/JP2016526378A/ja not_active Withdrawn
- 2014-06-05 US US14/895,993 patent/US20160122797A1/en not_active Abandoned
- 2014-06-05 EP EP14811476.2A patent/EP3008203A4/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432780A (zh) * | 2011-09-16 | 2012-05-02 | 复旦大学 | 一种表面羧基功能化的核壳式磁性复合微球及其制备方法 |
Non-Patent Citations (2)
| Title |
|---|
| S. SINGH ET AL.: "Surface engineered magnetic nanoparticles for removal of toxic metal ions and bacterial pathogens", 《JOURNAL OF HAZARDOUS MATERIALS》 * |
| SOPHIE LAURENT ET AL.: "Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization,Physicochemical Characterizations, and Biological Applications", 《CHEMICAL REVIEWS》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105954268A (zh) * | 2016-04-22 | 2016-09-21 | 华南师范大学 | 一种基于四氧化三铁纳米簇催化信号放大的可视化检测传感器及其构建与应用 |
| CN108496231A (zh) * | 2016-08-19 | 2018-09-04 | 阿莫生命科学有限公司 | 制备超顺磁性纳米复合材料的方法及使用该方法制备的超顺磁性纳米复合材料 |
| CN108496231B (zh) * | 2016-08-19 | 2021-07-30 | 阿莫生命科学有限公司 | 制备超顺磁性纳米复合材料的方法及使用该方法制备的超顺磁性纳米复合材料 |
| CN110501208A (zh) * | 2018-05-17 | 2019-11-26 | 国家纳米科学中心 | 叶酸功能化的链霉亲和素修饰的磁性纳米颗粒、其制备方法和应用 |
| CN110548489A (zh) * | 2019-09-12 | 2019-12-10 | 安徽工业大学 | 一种氨基磁性纳米粒子的制备方法及其在dna提取中的应用 |
| CN110548489B (zh) * | 2019-09-12 | 2022-06-24 | 安徽工业大学 | 一种氨基磁性纳米粒子的制备方法及其在dna提取中的应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014200790A1 (en) | 2014-12-18 |
| EP3008203A1 (en) | 2016-04-20 |
| EP3008203A4 (en) | 2017-01-25 |
| US20160122797A1 (en) | 2016-05-05 |
| JP2016526378A (ja) | 2016-09-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105308185A (zh) | 使用羧基官能化超顺磁性纳米团簇的磁性分离方法 | |
| JP6023698B2 (ja) | 試料からの低濃度の細菌の抽出 | |
| CN102027367B (zh) | 促进生物反应的屏障 | |
| JP5451623B2 (ja) | 微生物濃縮プロセス | |
| CN101907556B (zh) | 利用磁纳米颗粒富集结合双色流式细胞术检测大肠杆菌的方法 | |
| Son et al. | Quantitative DNA hybridization in solution using magnetic/luminescent core–shell nanoparticles | |
| EP2588863B1 (en) | Capture of micro-organisms | |
| Xu et al. | A fluorescent aptasensor coupled with nanobead-based immunomagnetic separation for simultaneous detection of four foodborne pathogenic bacteria | |
| US9766237B2 (en) | Method of capturing bacteria on polylysine-coated microspheres | |
| Zhao et al. | Rapid detection of Cronobacter sakazakii in dairy food by biofunctionalized magnetic nanoparticle based on nuclear magnetic resonance | |
| CN101458251A (zh) | 高通量检测生物大分子的磁性颗粒微阵列装置及其使用方法 | |
| Konry et al. | Particles and microfluidics merged: perspectives of highly sensitive diagnostic detection | |
| CN114966012A (zh) | 基于超顺磁性二维材料的低场核磁共振均相免疫检测食源性致病菌的方法 | |
| CN108300758A (zh) | 一种Hemin杂化纳米花及其制备方法和应用 | |
| Shirzad et al. | One-pot rapid visual detection of E. coli O157: H7 by label-free AuNP-based plasmonic-aptasensor in water sample | |
| Chang et al. | Comparing recovering efficiency of immunomagnetic separation and centrifugation of mycobacteria in metalworking fluids | |
| Zhang et al. | Functionalization of high-moment magnetic nanodisks for cell manipulation and separation | |
| Armstrong et al. | Magnetic capture device for large volume sample analysis | |
| Wang et al. | A broad-range method to detect genomic DNA of multiple pathogenic bacteria based on the aggregation strategy of gold nanorods | |
| US20170328900A1 (en) | Methods and materials for capture antibody targeted fluorescent in-situ hybridization (cat-fish) | |
| US9719989B2 (en) | Method for detecting food poisoning bacteria using magnetic nanoparticles and solution having high viscosity | |
| US10752892B2 (en) | Multilayer complex, method for manufacturing said complex and use of said complex | |
| Krizkova et al. | Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation: Rapid Superparamagnetic-Beads-Based Automated Immunoseparation of Zn-Proteins from Staphylococcus aureus with Nanogram Yield | |
| Stroot et al. | Capture antibody targeted fluorescence in situ hybridization (CAT-FISH): Dual labeling allows for increased specificity in complex samples | |
| Dester | Extraction, Concentration, and Detection of Foodborne Pathogens Using Glycan-Coated Magnetic Nanoparticles and a Gold Nanoparticle Colorimetric Biosensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160203 |
|
| RJ01 | Rejection of invention patent application after publication |