CN105283560B - 基于纳米孔的通过混合的fret检测的核酸分析 - Google Patents

基于纳米孔的通过混合的fret检测的核酸分析 Download PDF

Info

Publication number
CN105283560B
CN105283560B CN201480030047.0A CN201480030047A CN105283560B CN 105283560 B CN105283560 B CN 105283560B CN 201480030047 A CN201480030047 A CN 201480030047A CN 105283560 B CN105283560 B CN 105283560B
Authority
CN
China
Prior art keywords
nanopore
energy transfer
resonance energy
pore
fret
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201480030047.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN105283560A (zh
Inventor
马丁·胡伯
巴森·E·克兰西
保罗·哈登伯尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantapore Inc
Original Assignee
Quantapore Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantapore Inc filed Critical Quantapore Inc
Publication of CN105283560A publication Critical patent/CN105283560A/zh
Application granted granted Critical
Publication of CN105283560B publication Critical patent/CN105283560B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN201480030047.0A 2013-05-24 2014-05-23 基于纳米孔的通过混合的fret检测的核酸分析 Active CN105283560B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361827519P 2013-05-24 2013-05-24
US61/827,519 2013-05-24
PCT/US2014/039444 WO2014190322A2 (en) 2013-05-24 2014-05-23 Nanopore-based nucleic acid analysis with mixed fret detection

Publications (2)

Publication Number Publication Date
CN105283560A CN105283560A (zh) 2016-01-27
CN105283560B true CN105283560B (zh) 2018-11-30

Family

ID=51934368

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480030047.0A Active CN105283560B (zh) 2013-05-24 2014-05-23 基于纳米孔的通过混合的fret检测的核酸分析

Country Status (7)

Country Link
US (4) US9862997B2 (https=)
EP (1) EP3004385B1 (https=)
JP (2) JP6399610B2 (https=)
CN (1) CN105283560B (https=)
AU (1) AU2014268322B2 (https=)
CA (1) CA2910019A1 (https=)
WO (1) WO2014190322A2 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
CA2910019A1 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
ES2789000T3 (es) 2014-10-10 2020-10-23 Quantapore Inc Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente
CN107002126B (zh) 2014-10-24 2021-05-25 昆塔波尔公司 使用纳米结构阵列的聚合物的高效光学分析
WO2016200737A1 (en) * 2015-06-09 2016-12-15 Quantapore, Inc. Nanopore-based analysis of compounds using mobile fret pairs
CN108473933A (zh) * 2015-12-08 2018-08-31 昆塔波尔公司 使核酸移位通过纳米孔的方法
WO2017123647A1 (en) 2016-01-15 2017-07-20 Quantapore, Inc. Optically-based nanopore analysis with reduced background
WO2018081178A1 (en) * 2016-10-24 2018-05-03 Two Pore Guys, Inc. Fractional abundance of polynucleotide sequences in a sample
WO2017180319A1 (en) * 2016-04-14 2017-10-19 Quantapore, Inc. Mixed optical signals in polymer analysis with nanopores
EP3464612B8 (en) 2016-05-31 2024-12-11 Switchback Systems, Inc. Two-color nanopore sequencing
JP2019522983A (ja) * 2016-07-05 2019-08-22 クアンタポール， インコーポレイテッド 光学ベースのナノポア配列決定
CA3031812A1 (en) 2016-08-19 2018-02-22 Quantapore, Inc. Optically-based nanopore sequencing using quenching agents
JP2019528776A (ja) * 2016-10-10 2019-10-17 クアンタポール， インコーポレイテッド 光学に基づくナノポア配列決定における蛍光標識組成物の制御
JP7012841B2 (ja) * 2017-10-23 2022-01-28 エフ．ホフマン－ラ ロシュ アーゲー 浸透性の不均衡を利用した膜内のタンパク質ナノポアの除去および再挿入
JP7390027B2 (ja) 2017-10-31 2023-12-01 エンコディア， インコーポレイテッド 核酸エンコーディングおよび／または標識を使用する解析のためのキット
AU2019269615B2 (en) * 2018-05-17 2023-04-06 Recognition AnalytiX, Inc. Device, system and method for direct electrical measurement of enzyme activity
EP4273550A3 (en) 2018-05-17 2024-04-03 Northeastern University Lipid-free anchoring of thermophilic bacteriophage g20c portal adapter into solid-state nanopores
CN113166209B (zh) 2018-10-08 2025-01-07 弗里堡大学阿道夫梅克尔研究所 成孔肽的基于寡核苷酸的调节以增加孔尺寸、膜亲和性、稳定性和抗菌活性
DE102019200929A1 (de) * 2019-01-25 2020-07-30 Robert Bosch Gmbh Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls
US12351855B2 (en) 2019-01-30 2025-07-08 Arizona Board Of Regents On Behalf Of Arizona State University Bioelectronic circuits, systems and methods for preparing and using them
CN110243792A (zh) * 2019-06-11 2019-09-17 山东师范大学 一种基于量子点和四面体dna结构的荧光化学传感器及其检测方法和应用
CN110736775A (zh) * 2019-10-11 2020-01-31 深圳清华大学研究院 一种固态纳米孔修饰处理方法
IL297732A (en) 2020-04-30 2022-12-01 Univ Arizona State Methods for sequencing biopolymers
EP4102210A1 (en) * 2021-06-07 2022-12-14 Oxford University Innovation Limited Nanophotonic nanopore, memory system comprising a nanophotonic nanopore, and method of reading and/or writing information from and/or to an analyte
CN113533275B (zh) * 2021-06-30 2023-11-28 东南大学 固态纳米孔-荧光共振能量转移复合检测方法及系统

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010117470A2 (en) * 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CN102084001A (zh) * 2008-03-28 2011-06-01 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
CN102083998A (zh) * 2007-06-19 2011-06-01 斯特拉托斯基因公司 通过展开进行高通量核酸测序
WO2012121756A1 (en) * 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing

Family Cites Families (207)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH614781A5 (https=) 1977-06-27 1979-12-14 Contraves Ag
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US5131755A (en) 1988-02-19 1992-07-21 Chadwick Curt H Automatic high speed optical inspection system
JP3123249B2 (ja) 1991-09-10 2001-01-09 株式会社日立製作所 Ｄｎａ分子の長さ計測方法および計測装置
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
EP0636186B1 (en) 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Probe composition and method
US5387926A (en) 1992-06-30 1995-02-07 California Institute Of Technology High speed digital framing camera
US5798042A (en) 1994-03-07 1998-08-25 Regents Of The University Of California Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters
US5624845A (en) * 1995-03-16 1997-04-29 International Business Machines Corporation Assembly and a method suitable for identifying a code
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6794127B1 (en) 1997-06-16 2004-09-21 Diversa Corporation Capillary array-based sample screening
WO1997039150A1 (en) 1996-04-15 1997-10-23 University Of Southern California Synthesis of fluorophore-labeled dna
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
DE19714221A1 (de) 1997-04-07 1998-10-08 Zeiss Carl Fa Konfokales Mikroskop mit einem motorischen Scanningtisch
CZ297165B6 (cs) 1997-04-21 2006-09-13 Randox Laboratories Ltd. A British Company Of Ardmore Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
AU1603199A (en) 1997-12-03 1999-06-16 Curagen Corporation Methods and devices for measuring differential gene expression
EP1040349B2 (de) 1997-12-17 2012-12-19 Ecole Polytechnique Federale De Lausanne (Epfl) Positionierung und elektrophysiologische charakterisierung einzelner zellen und rekonstituierter membransysteme auf mikrostrukturierten trägern
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
CN1248741C (zh) 1997-12-23 2006-04-05 斯特里斯公司 具有集成过滤器的抗菌混合剂输送系统
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
DE19804372A1 (de) 1998-02-04 1999-08-05 Michael W Dr Dr Dahm Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits
GB9805301D0 (en) 1998-03-12 1998-05-06 Imperial College Detector
US6306589B1 (en) 1998-05-27 2001-10-23 Vysis, Inc. Biological assays for analyte detection
US6504943B1 (en) 1998-07-20 2003-01-07 Sandia Corporation Information-efficient spectral imaging sensor
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6790671B1 (en) 1998-08-13 2004-09-14 Princeton University Optically characterizing polymers
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6906749B1 (en) 1998-09-16 2005-06-14 Dalsa, Inc. CMOS TDI image sensor
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
EP1271154A3 (en) 1998-09-18 2005-08-17 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
US6252303B1 (en) 1998-12-02 2001-06-26 Advanced Micro Devices, Inc. Intergration of low-K SiOF as inter-layer dielectric
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
FR2788008B1 (fr) 1998-12-30 2001-03-23 Inst Curie Milieu thermosensible pour la separation electrocinetique d'especes au sein d'un canal de separation
WO2000043547A1 (en) 1999-01-20 2000-07-27 Northwestern University Dna mobility modifier
US6249341B1 (en) 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6975400B2 (en) 1999-01-25 2005-12-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6473176B2 (en) 1999-01-25 2002-10-29 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6671044B2 (en) 1999-01-25 2003-12-30 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells in broad flat flow
US6608682B2 (en) 1999-01-25 2003-08-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
WO2000056937A2 (en) 1999-03-25 2000-09-28 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
US20040214221A1 (en) 1999-05-07 2004-10-28 Klaus Muehlegger High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used
US7122152B2 (en) 1999-05-10 2006-10-17 University Of Florida Spatiotemporal and geometric optimization of sensor arrays for detecting analytes fluids
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US8206568B2 (en) 1999-06-22 2012-06-26 President And Fellows Of Harvard College Material deposition techniques for control of solid state aperture surface properties
EP2383776B1 (en) 1999-06-22 2015-02-25 President and Fellows of Harvard College Solid state nanopore device for evaluating biopolymers
US6464842B1 (en) 1999-06-22 2002-10-15 President And Fellows Of Harvard College Control of solid state dimensional features
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7105300A (en) 1999-09-03 2001-04-10 Lifebeam Technologies, Inc. Optical system for rapid polymer analysis
EP1212462B1 (en) 1999-09-07 2006-01-25 The Regents of the University of California Methods of determining the presence of double stranded nucleic acids in a sample
US6335420B1 (en) 1999-11-22 2002-01-01 Ibc Advanced Technologies, Inc. Polyamide ligand-containing polymeric resins and methods of using the same for removing, separating and/or concentrating desired metal ions from solutions
CA2398107C (en) 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
US20060292041A1 (en) 2000-03-23 2006-12-28 Dugas Matthew P Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US6616895B2 (en) 2000-03-23 2003-09-09 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US20050153284A1 (en) 2000-06-30 2005-07-14 Zeno Foldes-Papp Single molecule sequencing method
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US6608680B2 (en) 2000-08-25 2003-08-19 Amnis Corporation TDI imaging system for kinetic studies
US6583865B2 (en) 2000-08-25 2003-06-24 Amnis Corporation Alternative detector configuration and mode of operation of a time delay integration particle analyzer
US6934408B2 (en) 2000-08-25 2005-08-23 Amnis Corporation Method and apparatus for reading reporter labeled beads
AU2001293232A1 (en) 2000-08-29 2002-03-13 The Rockefeller University Methods employing fluorescence quenching by metal surfaces
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US6998251B2 (en) 2001-01-12 2006-02-14 Syngenta Participations Ag Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics
US6743905B2 (en) 2001-04-16 2004-06-01 Applera Corporation Mobility-modified nucleobase polymers and methods of using same
KR100889351B1 (ko) 2001-05-15 2009-03-18 가부시키가이샤 에바라 세이사꾸쇼 Ｔｄｉ 검출 디바이스, 피드-스루 장비 및 이들 디바이스를 이용하는 전자빔 장치
US20040175710A1 (en) 2001-05-22 2004-09-09 Haushalter Robert C. Method for in situ, on-chip chemical synthesis
US6618140B2 (en) 2001-06-18 2003-09-09 Amnis Corporation Spectral deconvolution of fluorescent markers
US7009163B2 (en) 2001-06-22 2006-03-07 Orbotech Ltd. High-sensitivity optical scanning using memory integration
WO2003003015A2 (en) 2001-06-28 2003-01-09 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
US7280207B2 (en) 2001-07-25 2007-10-09 Applera Corporation Time-delay integration in a flow cytometry system
WO2003010289A2 (en) 2001-07-25 2003-02-06 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
JP2004537053A (ja) 2001-07-25 2004-12-09 アプレラ コーポレイション 電気泳動検出システムにおける時間遅延積分
US20080288178A1 (en) 2001-08-24 2008-11-20 Applera Corporation Sequencing system with memory
US6627433B2 (en) 2001-08-24 2003-09-30 Applera Corporation Multi-channel analyte-separation device employing side-entry excitation
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6706203B2 (en) 2001-10-30 2004-03-16 Agilent Technologies, Inc. Adjustable nanopore, nanotome, and nanotweezer
DE10154291B4 (de) 2001-11-05 2005-05-19 Hain Lifescience Gmbh Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes
DE10162535A1 (de) 2001-12-19 2003-07-03 Gnothis Holding Sa Ecublens Sequenzierung über Lochmembranen
US7008547B2 (en) 2002-03-14 2006-03-07 Sarnoff Corporation Solid phase sensors
JP3752466B2 (ja) 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
US7744816B2 (en) 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
US8278055B2 (en) 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
EP1504114B1 (en) 2002-05-10 2017-07-12 The Texas A & M University System Stochastic sensing through covalent interactions
US7005264B2 (en) 2002-05-20 2006-02-28 Intel Corporation Method and apparatus for nucleic acid sequencing and identification
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
US6952651B2 (en) 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
TWI234584B (en) 2002-08-16 2005-06-21 Univ Nat Taiwan Normal Nucleic acid sequencing method
WO2004029580A2 (en) 2002-09-25 2004-04-08 Amersham Biosciences (Sv) Corp High density fluidic chip design and method of sample injection
AU2003301244A1 (en) 2002-10-15 2004-05-04 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
CN1161613C (zh) 2002-10-22 2004-08-11 中国人民解放军南京军区联勤部军事医学研究所 免疫细胞微流体阵列
US7390457B2 (en) 2002-10-31 2008-06-24 Agilent Technologies, Inc. Integrated microfluidic array device
US20040137158A1 (en) 2003-01-15 2004-07-15 Kools Jacques Constant Stefan Method for preparing a noble metal surface
US7410564B2 (en) 2003-01-27 2008-08-12 Agilent Technologies, Inc. Apparatus and method for biopolymer identification during translocation through a nanopore
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
WO2004085609A2 (en) 2003-02-28 2004-10-07 Brown University Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same
US7250115B2 (en) 2003-06-12 2007-07-31 Agilent Technologies, Inc Nanopore with resonant tunneling electrodes
US7248771B2 (en) 2003-06-16 2007-07-24 Brigham Young University Integrated sensor with electrical and optical single molecule sensitivity
US7444053B2 (en) 2003-06-16 2008-10-28 The Regents Of The University Of California Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity
WO2005017025A2 (en) 2003-08-15 2005-02-24 The President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US7075161B2 (en) 2003-10-23 2006-07-11 Agilent Technologies, Inc. Apparatus and method for making a low capacitance artificial nanopore
US20050095599A1 (en) 2003-10-30 2005-05-05 Pittaro Richard J. Detection and identification of biopolymers using fluorescence quenching
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
US7271896B2 (en) 2003-12-29 2007-09-18 Intel Corporation Detection of biomolecules using porous biosensors and raman spectroscopy
US20050164211A1 (en) 2004-01-22 2005-07-28 Hannah Eric C. Carbon nanotube molecular labels
WO2005071110A2 (en) 2004-01-23 2005-08-04 Lingvitae As Improving polynucleotide ligation reactions
US20050186576A1 (en) 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
US7279337B2 (en) 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US20050227239A1 (en) 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
US20070264623A1 (en) 2004-06-15 2007-11-15 President And Fellows Of Harvard College Nanosensors
US20060019259A1 (en) 2004-07-22 2006-01-26 Joyce Timothy H Characterization of biopolymers by resonance tunneling and fluorescence quenching
JP4897681B2 (ja) 2004-07-23 2012-03-14 エレクトロニック・バイオサイエンシーズ・エルエルシー イオン・チャネルを通過する時間的に変化する電流を検出するための方法及び装置
US20070054276A1 (en) 2004-08-12 2007-03-08 Sampson Jeffrey R Polynucleotide analysis and methods of using nanopores
US7972858B2 (en) 2004-08-13 2011-07-05 President And Fellows Of Harvard College Ultra high-throughput opti-nanopore DNA readout platform
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006137891A2 (en) 2004-09-29 2006-12-28 University Of Florida Research Foundation, Inc. Membrane with nanochannels for detection of molecules
US7371533B2 (en) 2004-10-05 2008-05-13 University Of Ottawa Methods for separation of polymeric compounds
WO2006052882A1 (en) 2004-11-09 2006-05-18 President And Fellows Of Harvard College Formation of eddies in constrained fluidic channels and uses thereof
US7609309B2 (en) 2004-11-18 2009-10-27 Kla-Tencor Technologies Corporation Continuous clocking of TDI sensors
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060210995A1 (en) 2005-03-15 2006-09-21 Joyce Timothy H Nanopore analysis systems and methods of using nanopore devices
ATE529734T1 (de) 2005-04-06 2011-11-15 Harvard College Molekulare charakterisierung mit kohlenstoff- nanoröhrchen-steuerung
US20060231419A1 (en) 2005-04-15 2006-10-19 Barth Philip W Molecular resonant tunneling sensor and methods of fabricating and using the same
US7879575B2 (en) 2005-04-27 2011-02-01 The Trustees Of The University Of Pennsylvania Nanostructures that provide a modified nanoenvironment for the enhancement of luminescence
EP1721657A1 (en) 2005-05-13 2006-11-15 SONY DEUTSCHLAND GmbH A method of fabricating a polymeric membrane having at least one pore
JP2007006720A (ja) 2005-06-28 2007-01-18 Toshiba Corp 個体識別方法、並びに個体識別検査のためのアレイ、装置及びシステム
US20090148348A1 (en) 2005-08-11 2009-06-11 Eksigent Technologies, Llc Plastic surfaces and apparatuses for reduced adsorption of solutes and methods of preparing the same
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007041340A2 (en) 2005-09-30 2007-04-12 The Trustees Of Columbia University In The City Of New York Microfluidic cells with parallel arrays of individual dna molecules
WO2007041621A2 (en) 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
KR100721430B1 (ko) 2005-10-12 2007-05-23 학교법인 포항공과대학교 나노다공성 멤브레인 및 이의 제조방법
US7397232B2 (en) 2005-10-21 2008-07-08 The University Of Akron Coulter counter having a plurality of channels
US7835870B2 (en) 2005-11-01 2010-11-16 Georgia Institute Of Technology Methods and systems for evaluating the length of elongated elements
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
DE602006016984D1 (de) 2005-12-12 2010-10-28 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
EP1987275A1 (en) 2006-02-13 2008-11-05 Koninklijke Philips Electronics N.V. Microfluidic device for molecular diagnostic applications
US20070224613A1 (en) 2006-02-18 2007-09-27 Strathmann Michael P Massively Multiplexed Sequencing
US20070202008A1 (en) 2006-02-28 2007-08-30 Schembri Carol T Systems and methods of lipoprotein size fraction assaying
US20070215472A1 (en) 2006-03-15 2007-09-20 Slater Gary W Electroosmotic flow for end labelled free solution electrophoresis
US20070218494A1 (en) 2006-03-17 2007-09-20 Gary Slater Branched polymer lables as drag-tags in free solution electrophoresis
US7777505B2 (en) 2006-05-05 2010-08-17 University Of Utah Research Foundation Nanopore platforms for ion channel recordings and single molecule detection and analysis
US7849581B2 (en) 2006-05-05 2010-12-14 University Of Utah Research Foundation Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof
JP2009543058A (ja) 2006-06-30 2009-12-03 アプライド バイオシステムズ， エルエルシー 結合相互作用を分析する方法
US7553730B2 (en) 2006-07-14 2009-06-30 Agilent Technologies, Inc. Methods of fabrication employing nanoscale mandrels
US20080032290A1 (en) 2006-08-03 2008-02-07 Young James E Nanopore flow cells
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
WO2008049795A1 (en) 2006-10-23 2008-05-02 Flexgen Bv Method and system for calibrating laser focus and position in micro-arrays
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US7544578B2 (en) 2007-01-03 2009-06-09 International Business Machines Corporation Structure and method for stochastic integrated circuit personalization
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US20100035268A1 (en) 2007-02-21 2010-02-11 Joseph Beechem Materials and methods for single molecule nucleic acid sequencing
US20080254995A1 (en) 2007-02-27 2008-10-16 Drexel University Nanopore arrays and sequencing devices and methods thereof
EP2156179B1 (en) 2007-04-04 2021-08-18 The Regents of The University of California Methods for using a nanopore
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
CA2689626C (en) 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
GB0713143D0 (en) 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
US8273532B2 (en) 2007-10-02 2012-09-25 President And Fellows Of Harvard College Capture, recapture, and trapping of molecules with a nanopore
GB0721340D0 (en) 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
US20110165652A1 (en) * 2008-01-14 2011-07-07 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
US8852864B2 (en) 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
WO2009108914A1 (en) 2008-02-28 2009-09-03 Electronic Bio Sciences, Llc System and method to improve sequencing accuracy of a polymer
CN102084003A (zh) 2008-04-04 2011-06-01 生命科技公司 用于成像和测序的扫描系统和方法
US20110172404A1 (en) 2008-05-19 2011-07-14 Cornell University Self-Assembly of Nanoparticles Through Nuclei Acid Engineering
US8013300B2 (en) 2008-06-20 2011-09-06 Carl Zeiss Nts, Llc Sample decontamination
US8628919B2 (en) 2008-06-30 2014-01-14 Bionano Genomics, Inc. Methods and devices for single-molecule whole genome analysis
EP2310534B1 (en) 2008-07-07 2018-09-05 Oxford Nanopore Technologies Limited Base-detecting pore
ATE544063T1 (de) 2008-07-17 2012-02-15 Koninkl Philips Electronics Nv Nanoporenvorrichtung und verfahren zur nukleinsäureanalyse
CA2774710C (en) 2008-09-22 2016-08-02 University Of Washington Msp nanopores and related methods
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CN201302544Y (zh) 2008-12-04 2009-09-02 浙江大学 高俘获率高灵敏度的微流控spr生物传感装置
WO2010080617A2 (en) 2008-12-19 2010-07-15 The Board Of Trustees Of The University Of Illinois Detecting and sorting methylated dna using a synthetic nanopore
CA2756233A1 (en) 2009-03-26 2010-09-30 Trustees Of Boston University Method for imaging on thin solid-state interface between two fluids
JP5372570B2 (ja) 2009-03-30 2013-12-18 株式会社日立ハイテクノロジーズ ナノポアを用いたバイオポリマー決定方法、システム、及びキット
WO2010129869A1 (en) 2009-05-07 2010-11-11 The Trustees Of Boston University Manufacture of nanoparticles using nanopores and voltage-driven electrolyte flow
US8926904B2 (en) 2009-05-12 2015-01-06 Daniel Wai-Cheong So Method and apparatus for the analysis and identification of molecules
US9255002B2 (en) 2009-09-22 2016-02-09 Cornell University Apparatus and method for forming self-assembly arrays
EP2483680A4 (en) * 2009-09-30 2014-01-01 Quantapore Inc ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE
US20140335513A9 (en) 2009-09-30 2014-11-13 Quantapore, Inc. Hybrid nanopore device with optical detection and methods of using same
EP2491138A1 (en) 2009-10-21 2012-08-29 Bionano Genomics, Inc. Methods and related devices for single molecule whole genome analysis
US9127313B2 (en) 2009-12-01 2015-09-08 Oxford Nanopore Technologies Limited Biochemical analysis instrument
CN102918166A (zh) 2010-03-30 2013-02-06 波士顿大学董事会 用于纳米孔解链依赖性核酸测序的工具和方法
US20130256118A1 (en) 2010-05-11 2013-10-03 Trustees Of Boston University Use of Nanopore Arrays For Multiplex Sequencing of Nucleic Acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
CN103069267B (zh) 2010-09-29 2016-05-11 株式会社日立高新技术 生物聚合物的光学的解析装置以及方法
WO2012170499A2 (en) 2011-06-06 2012-12-13 The University Of North Carolina At Greensboro Nanopore fabrication and applications thereof
US20130040827A1 (en) 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
GB2497784A (en) 2011-12-20 2013-06-26 Base4 Innovation Ltd Identification of polymers using nanopore devices
GB201212135D0 (en) 2012-07-09 2012-08-22 Base4 Innovation Ltd Improved sequencing apparatus
WO2014066905A2 (en) 2012-10-28 2014-05-01 Quantapore, Inc. Reducing background fluorescence in mems materials by low energy ion beam treatment
CA2910019A1 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
CN107002126B (zh) * 2014-10-24 2021-05-25 昆塔波尔公司 使用纳米结构阵列的聚合物的高效光学分析

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102083998A (zh) * 2007-06-19 2011-06-01 斯特拉托斯基因公司 通过展开进行高通量核酸测序
CN102084001A (zh) * 2008-03-28 2011-06-01 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
WO2010117470A2 (en) * 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
WO2012121756A1 (en) * 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
纳米孔单分子DNA检测方法及人造纳米孔的制备;司儆舟等;《国际生物医学工程杂志》;20091231;第32卷(第6期);353-358 *

Also Published As

Publication number Publication date
US20160076091A1 (en) 2016-03-17
AU2014268322A1 (en) 2015-10-29
US9862997B2 (en) 2018-01-09
JP2018099141A (ja) 2018-06-28
EP3004385A2 (en) 2016-04-13
CN105283560A (zh) 2016-01-27
WO2014190322A3 (en) 2015-01-22
AU2014268322B2 (en) 2019-01-24
WO2014190322A2 (en) 2014-11-27
US20190360035A1 (en) 2019-11-28
JP2016524469A (ja) 2016-08-18
EP3004385B1 (en) 2018-11-28
US20180073072A1 (en) 2018-03-15
CA2910019A1 (en) 2014-11-27
US20210130887A1 (en) 2021-05-06
EP3004385A4 (en) 2017-02-22
JP6399610B2 (ja) 2018-10-10

Similar Documents

Publication Publication Date Title
CN105283560B (zh) 基于纳米孔的通过混合的fret检测的核酸分析
US11041197B2 (en) Efficient optical analysis of polymers using arrays of nanostructures
US20220349000A1 (en) Nanopore-based polymer analysis with mutually-quenching fluorescent labels
US20250320543A1 (en) Method of translocating nucleic acids through nanopores

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant