CN105249098A - 一种紫薯乳酸菌饮料的制作方法 - Google Patents
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Abstract
本发明涉及一种紫薯乳酸菌饮料的制作方法,包括:(1)紫薯汁的制备,(2)菌种的活化与驯化,(3)紫薯乳酸菌饮料的发酵。本发明以紫薯为主要发酵基质,利用五步法驯化的复合乳酸菌为发酵剂,采用特有的培养基进行发酵,制备出具有改善肠道功能、抗氧化等功能的紫薯功能性乳酸菌饮料。产品香味独特,营养价值高。
Description
技术领域
本发明属于食品加工技术领域,具体是一种以紫薯为主要原料的乳酸菌饮料。
背景技术
乳酸菌饮料是一种风味独特的发酵乳饮料,集口感、营养于一身,具备调配型酸乳饮料所没有的优点。乳酸菌饮料是向培养基中接入乳酸菌进行发酵制得,部分乳糖转化为乳酸,有利于乳糖不耐受症人群的食用,同时蛋白则被转化为多种氨基酸、肽等,可促进人体的消化吸收,并生成多种呈味物质,致使产品具备更好的风味及口感。现有乳酸菌发酵饮料中,以牛奶为发酵基料较多,也有研究采用大豆等与牛奶调配的,但是产品较少,而以紫薯为基料的还很鲜见。
发明内容
为了丰富紫薯的产品形式、提升紫薯的加工附加值,本发明提供一种紫薯乳酸菌饮料的制作方法。
一种紫薯乳酸菌饮料的制作方法,包括以下步骤:
(1)紫薯汁的制备:称取100g去皮的紫薯肉,加入1000mL蒸馏水,捣碎混匀,再加入18-20g的牛乳粉,70-80g的蔗糖,2-3g的高甲氧基果胶,1-3g黄原胶,2-3g羧甲基纤维素钠,混匀,调节pH至6.0-6.2,95℃高压灭菌10min,立刻冷却至40℃,得到紫薯汁;
(2)紫薯乳酸菌饮料的发酵:取100g紫薯汁,加入活化及驯化后的复合乳酸菌3-4g,在38-40℃条件下培养7-8h,所得发酵液即为紫薯乳酸菌饮料。
其中,复合乳酸菌活化与驯化的方法如下:
(1)将1.0×1015-1.0×1016CFU/g的嗜热链球菌0.04-0.06g和1.0×1014-1.0×1015CFU/g的保加利亚乳杆菌0.04-0.06g接种于脱脂乳培养基中,38-40℃环境下培养至酸度达到70-80°T时,完成活化;
(2)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入100g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入活化后的菌种,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅰ;
(3)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入80g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅰ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅱ;
(4)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入60g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅱ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅲ;
(5)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入40g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅲ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅳ;
(6)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入20g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅳ,随即置于38-40℃环境下驯化培养12h,制得复合乳酸菌。
本发明的有益技术效果体现在:
(1)当发酵基质中含有足够的营养成分,乳酸菌才能正常生长和大量繁殖,因此本发明采用五步法降低培养基中奶粉的浓度来进行驯化培养,使乳酸菌逐渐适应紫薯汁的生长环境,提高酸度,使驯化后的酸度比驯化前显著性增长。乳酸菌发酵环境是一种不稳定的混合体系,故控制发酵稳定性是本发明的独特之处。
(2)本发明采用复合果胶、黄原胶和羧甲基纤维素钠强化紫薯乳液,充分利用几种营养稳定剂之间的协同作用,有利于紫薯乳酸菌饮料的发酵。
具体实施方式
下面通过实施例,对本发明作进一步说明。
实施例1
(1)紫薯汁的制备:称取100g去皮的紫薯肉,加入1000mL蒸馏水,捣碎混匀,再加入20g的牛乳粉,80g的蔗糖,3g的高甲氧基果胶,3g黄原胶,3g羧甲基纤维素钠,混匀,调节pH至6.0,95℃高压灭菌10min,立刻冷却至40℃,得到紫薯汁;
(2)紫薯乳酸菌饮料的发酵:取100g紫薯汁,加入活化及驯化后的复合乳酸菌4g,在40℃条件下培养8h,所得发酵液即为紫薯乳酸菌饮料。
其中,复合乳酸菌活化与驯化的方法如下:
(1)将1.0×1016CFU/g的嗜热链球菌0.06g和1.0×1014CFU/g的保加利亚乳杆菌0.05g接种于脱脂乳培养基中,40℃环境下培养至酸度达到80°T时,完成活化;
(2)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入100g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入活化后的菌种,随即置于40℃环境下驯化培养12h,制得发酵剂Ⅰ;
(3)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入80g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅰ,随即置于40℃环境下驯化培养12h,制得发酵剂Ⅱ;
(4)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入60g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅱ,随即置于40℃环境下驯化培养12h,制得发酵剂Ⅲ;
(5)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入40g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅲ,随即置于40℃环境下驯化培养12h,制得发酵剂Ⅳ;
(6)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入20g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅳ,随即置于40℃环境下驯化培养12h,制得复合乳酸菌。
Claims (2)
1.一种紫薯乳酸菌饮料的制作方法,其特征在于包括以下步骤:
(1)紫薯汁的制备:称取100g去皮的紫薯肉,加入1000mL蒸馏水,捣碎混匀,再加入18-20g的牛乳粉,70-80g的蔗糖,2-3g的高甲氧基果胶,1-3g黄原胶,2-3g羧甲基纤维素钠,混匀,调节pH至6.0-6.2,95℃高压灭菌10min,立刻冷却至40℃,得到紫薯汁;
(2)紫薯乳酸菌饮料的发酵:取100g紫薯汁,加入活化及驯化后的复合乳酸菌3-4g,在38-40℃条件下培养7-8h,所得发酵液即为紫薯乳酸菌饮料。
2.根据权利要求1所述的一种紫薯乳酸菌饮料的制作方法,其特征在于,活化及驯化后的复合乳酸菌的制备方法如下:
(1)将1.0×1015-1.0×1016CFU/g的嗜热链球菌0.04-0.06g和1.0×1014-1.0×1015CFU/g的保加利亚乳杆菌0.04-0.06g接种于脱脂乳培养基中,38-40℃环境下培养至酸度达到70-80°T时,完成活化;
(2)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入100g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入活化后的菌种,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅰ;
(3)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入80g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅰ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅱ;
(4)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入60g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅱ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅲ;
(5)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入40g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅲ,随即置于38-40℃环境下驯化培养12h,制得发酵剂Ⅳ;
(6)紫薯洗净、去皮后称取100g,加入1000mL蒸馏水、捣碎,加入20g牛乳粉、80g的蔗糖、3g的高甲氧基果胶、3g黄原胶和3g羧甲基纤维素钠,混匀,95℃杀菌10min,冷却至40℃后,接入发酵剂Ⅳ,随即置于38-40℃环境下驯化培养12h,制得复合乳酸菌。
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CN106070656A (zh) * | 2016-06-23 | 2016-11-09 | 徐州工程学院 | 一种紫薯乳酸菌复合饮料及其制备方法 |
CN106173652A (zh) * | 2016-07-11 | 2016-12-07 | 湖北省农业科学院农产品加工与核农技术研究所 | 红薯益生菌饮料及其制作方法 |
CN106306951A (zh) * | 2016-08-24 | 2017-01-11 | 徐州工程学院 | 一种多菌联合发酵紫薯复合饮料制备方法 |
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CN104082413A (zh) * | 2014-06-13 | 2014-10-08 | 宁波大学 | 一种无糖型紫薯酸奶的制备方法 |
CN104472705A (zh) * | 2014-11-26 | 2015-04-01 | 苏州苏东庭生物科技有限公司 | 一种紫薯酸乳饮料的加工方法 |
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CN104082413A (zh) * | 2014-06-13 | 2014-10-08 | 宁波大学 | 一种无糖型紫薯酸奶的制备方法 |
CN104472705A (zh) * | 2014-11-26 | 2015-04-01 | 苏州苏东庭生物科技有限公司 | 一种紫薯酸乳饮料的加工方法 |
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CN106070656A (zh) * | 2016-06-23 | 2016-11-09 | 徐州工程学院 | 一种紫薯乳酸菌复合饮料及其制备方法 |
CN106173652A (zh) * | 2016-07-11 | 2016-12-07 | 湖北省农业科学院农产品加工与核农技术研究所 | 红薯益生菌饮料及其制作方法 |
CN106306951A (zh) * | 2016-08-24 | 2017-01-11 | 徐州工程学院 | 一种多菌联合发酵紫薯复合饮料制备方法 |
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