CN105230573A - Indoor cultivation method for ostracoda - Google Patents

Abstract

The invention discloses an indoor cultivation method for ostracoda. In indoor conditions, ostracoda is put in a transparent glass ware, aeration or boiled water which has been put for a while is used as cultivation water, clean and high-temperature disinfected fine sandstones are used as a water-bed matrix where the ostracoda can breed and inhabit, chlorella is used as nutrients needed by vital movement of the ostracoda, then the glass ware is put in a place where is sufficient in sunlight but without direct sunlight, the indoor temperature is controlled within the range from 24 to 26 DEG C during cultivation. The ostracoda can normally breed, and the vitality is vigorous in the above condition. The indoor cultivation method for the ostracoda is low in cost, and easy to operate, a technical issue that most of the ostracoda species can hardly be cultivated indoor can be solved, and base condition guarantee can be provided to scientific research on the ostracoda in future.

Description

A kind of indoor culture method of mussel-shrimp

Technical field

The present invention relates to aquatic animal cultural technique field, particularly relate to the cultural method of miniature crustacean mussel-shrimp.

Background technology

Ostracode animal is under the jurisdiction of Arthropoda Crustacea, and its shell is long general in 0.5 ~ 2.0 millimeter, has bag that carapace extends to form by the bivalve of whole body, and external form is rolled up between bivalve as a curling shrimp.Namely mussel-shrimp occurred from the Paleozoic Era, was an ancient monoid in crustacean, and today is still flourishing in various water body, was the common a kind of mini-Frac test of water body.The calcareous housing of mussel-shrimp is easy to be kept in each geochronic stratum, therefore it has abundant fossil record, the fossil species recorded at present has an appointment 30,000 kind, and they are in the researchs such as oil detection, ancient lake transition, geological environment succession important " information carrier ".In addition, except species enrich, individual small and fossil species and recent species all easily obtain except a large amount of colony's samples, mussel-shrimp also has shorter single generation cycle, and batch (-type) is casted off a skin growth, and ontogeny is easily followed the trail of and evolve the feature such as stable.Because these features can compare favourably with fruit bat, so some scholars thinks that mussel-shrimp is " model animal that in Study on Evolution, application potential is huge ", there is the good reputation of " in water fruit bat ".These species are widely applied in the biological study of organs of vision evolution, modes of reproduction succession and bioluminescence Forming Mechanism etc. at present.But so far, rarely have report about mussel-shrimp Laboratory culture successful story, which has limited the performance of mussel-shrimp in research field effects such as evolution biologies.Therefore, build mussel-shrimp indoor culture system, can be and carry out the research such as experimental ecology, evolution biology using it as experiment material basic condition guarantee is provided; The cultivation system of standards and norms, also can increase the comparativity of each laboratory research achievement simultaneously.

Summary of the invention

The method of the indoor culture mussel-shrimp that object of the present invention aims to provide a kind of easy enforcement, easily promotes, cost is low, effective.

The object of the present invention is achieved like this:

An indoor culture method for mussel-shrimp, is characterized in that the method comprises the steps:

(1) structure of breeding environment

Get transparent glass vessels, it is cleaned with running water after the liquor potassic permanganate that concentration is 0.01-0.05% soaks 1-3 days, then carries out autoclave sterilization and dry; Get 30 object fine sand stones cleaned and carry out autoclave sterilization, be paved with bottom glassware after oven dry, thickness is 1-2cm, according to the difference of Ostracode species, in glassware, inject aeration or the running water after boiling, as the breeding water body of freshwater species, or aeration or the running water after boiling are mixed with the salt water that concentration is 2-7 ‰, as the breeding water body of salt water species, water level distance vessel open place 3-4cm; The glassware building breeding water body is placed in the indoor that temperature is 24-28 DEG C, is positioned over bright and clear, but without the position of direct sunlight;

(2) input of nutriment

A, in the glassware building breeding water body, add calcium chloride solution, make the concentration of calcium ion in glassware be 70-200mg/L, calcareous with what maintain required for mussel-shrimp growth and shelling;

B, drop in the glassware building breeding water body chlorella pyrenoidosa ( chlorellapyrenoidosa) algae liquid as the nutriment of mussel-shrimp, the volume ratio of algae liquid and water body is 1:100;

(3) maintenance of breeding water body

Within 6-8 days, supplement an aquaculture water, make water body remain limpid bright, maintain comparatively fixing water level; In addition, fresh chlorella algae liquid within 6-8 days, is supplemented once; Wherein:

Described running water aeration at least 5 days, boiling tap water places cooling at least 1 day after 10 minutes; Described fine sand stone at 121 DEG C, steam sterilizing 20 minutes under the condition of 100KPa.

The invention has the beneficial effects as follows :filled up the blank lacking mussel-shrimp indoor culture technology in the world, solve the difficult problem that when application mussel-shrimp carries out biological study, experiment material cannot ensure, the present invention is without the need to professional equipment, non-environmental-pollution, simple to operate, cultivating condition is controlled.

Embodiment

Below in conjunction with specific embodiment, clear, complete description is carried out to technical scheme of the present invention.

Embodiment 1

Nothing even bucket star is situated between ( cypridopsisvidua) indoor culture method, comprise the following steps:

(1) structure of breeding environment

Get the beaker of 1 liter, concentration be 0.03% liquor potassic permanganate soak after 1 day with running water clean, then at 121 DEG C, sterilizing 20 minutes under the condition of 100KPa is also dried stand-by, dry after the 30 order fine sand stones cleaned are added water boil, join after oven dry in beaker, make the thickness of microlith sand in beaker be about 1 ㎝.By abundant for running water aeration after 5 days, join in beaker and make liquid level concordant with the graduation mark of 1000 milliliters on beaker.By airconditioning control room temperature at 25 DEG C, will cultivate object fresh water species mussel-shrimp-nothing even bucket star be situated between ( cypridopsisvidua) put into beaker after, beaker is placed into light better, but without the place of direct sunlight.

(2) input of nutriment

A, in beaker, add appropriate calcium chloride solution, make the concentration of calcium ion be 150mg/L.

B, in beaker, add the algae liquid of 10ml chlorella pyrenoidosa.

(3) maintenance of breeding water body

Within one week, supplement an aquaculture water, make water body remain limpid bright, maintain comparatively fixing water level; In addition, the fresh chlorella algae liquid of 2-3ml within one week, is supplemented.

Embodiment 2

Unexpected lake flower is situated between ( limnocythereinopinata) indoor culture method, comprise the following steps:

(1) structure of breeding environment

Get the glassware of 5 liters, clean with running water after soaking 3 days with the liquor potassic permanganate that concentration is 0.05%, then carry out autoclave sterilization and dry stand-by, by 30 clean order fine sand stones at 121 DEG C, steam sterilizing 20 minutes under the condition of 100KPa; Join after oven dry in beaker, addition is as the criterion to be paved with beaker bottom.Boiling tap water was cooled to room temperature after 10 minutes, places after at least 1 day, join in glassware.In glassware, add appropriate using sea area for aquatic breeding salt, the salinity in water is controlled about 6 ‰.Put into cultivation object salt water kind mussel-shrimp---unexpected lake flower is situated between ( limnocythereinopinata) after, put into by glassware in constant temperature illumination box, intensity of illumination is set as 2500-3000LX, and temperature is set to 25 DEG C, humidity 60%, and Light To Dark Ratio is 12h:12h.

(2) input of nutriment

A, in glassware, add a certain amount of calcium chloride solution, make the concentration of calcium ion in beaker be about 120mg/L.

B, in glassware, add the algae liquid of 40ml chlorella pyrenoidosa.

(3) maintenance of breeding water body

Within one week, supplement an aquaculture water, make water body remain limpid bright, maintain comparatively fixing water level; In addition, the fresh chlorella algae liquid of 3-5ml within one week, is supplemented.

Claims (1)

1. an indoor culture method for mussel-shrimp, is characterized in that the method comprises the steps:

(1) structure of breeding environment

Get transparent glass vessels, it is cleaned with running water after the liquor potassic permanganate that concentration is 0.01-0.05% soaks 1-3 days, then carries out autoclave sterilization and dry; Get 30 object fine sand stones cleaned and carry out autoclave sterilization, be paved with bottom glassware after oven dry, thickness is 1-2cm, according to the difference of Ostracode species, in glassware, inject aeration or the running water after boiling, as the breeding water body of freshwater species, or aeration or the running water after boiling are mixed with the salt water that concentration is 2-7 ‰, as the breeding water body of salt water species, water level distance vessel open place 3-4cm; The glassware building breeding water body is placed in the indoor that temperature is 24-28 DEG C, is positioned over bright and clear, but without the position of direct sunlight;

(2) input of nutriment

A, in the glassware building breeding water body, add calcium chloride solution, make the concentration of calcium ion in glassware be 70-200mg/L, calcareous with what maintain required for mussel-shrimp growth and shelling;

B, drop in the glassware building breeding water body chlorella pyrenoidosa ( chlorellapyrenoidosa) algae liquid as the nutriment of mussel-shrimp, the volume ratio of algae liquid and water body is 1:100;

(3) maintenance of breeding water body

Within 6-8 days, supplement an aquaculture water, make water body remain limpid bright, maintain comparatively fixing water level; In addition, fresh chlorella algae liquid within 6-8 days, is supplemented once; Wherein:

Described running water aeration at least 5 days, boiling tap water places cooling at least 1 day after 10 minutes; Described fine sand stone at 121 DEG C, steam sterilizing 20 minutes under the condition of 100KPa.