CN105193881A - Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract as well as preparation method and application thereof - Google Patents
Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract, a cosmetic additive applying the Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract for realizing skin whitening, moisturizing and antioxidant activity and a combined application of the Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract in cosmetics. The Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract is ethanol extract of Bergenia purpurascens(Hook.f.et Thoms.)Engl. rhizomes and has good DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity and good tyrosinase activity inhibition effect. The Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract has good antioxidant capacity and melanin removal capacity in the aspect of skin care, that is, the Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract has good skin cosmetic activity application potential in aspects of anti-wrinkle, sunscreen, whitening, melanin (spot) removal. With effective use of Bergenia purpurascens(Hook.f.et Thoms.)Engl. which is the Naxi traditional medicinal plant, the Bergenia purpurascens(Hook.f.et Thoms.)Engl. extract with very high biological activity is obtained, can be used for preparing good antioxidant and tyrosinase activity inhibitor and is a good raw material for herbal cosmetics.
Description
Technical field
The invention belongs to cosmetics and medical art, be specifically related to extract natural active matter-Rhizoma Seu Herba Bergeniae extract from a kind of Naxi traditional drugs phytolith Chinese cabbage, the cosmetics being active component with it or medicine, and the application of Rhizoma Seu Herba Bergeniae extract in the various skin cosmetics such as preparation moisturizing, skin care, crease-resistant, sun-proof, whitening, also have in preparation treatment or prevent in dermopathic medicine application
Technical background
Rhizoma Seu Herba Bergeniae (Bergeniapurpurascens (Hook.f.etThoms.) Engl.), has another name called triaenophora rupestris, short Chinese cabbage, is Saxifragaceae Bergenia plant, and rhizome is thick, more piece, raw meat matter fibrous root.Phyllopodium is raw, film quality, blade obovate meat, raceme, floral white or pink.Rhizoma Seu Herba Bergeniae mainly distributes height above sea level more than 3700 meters, happiness warm and moist and half cloudy environment, and tolerance to cold is strong, is afraid of high temperature and high light, not drought-resistant.
The Land use systems of Rhizoma Seu Herba Bergeniae is common is medicinal, is the traditional trauma hemostasis medicine of Naxi.Rhizoma Seu Herba Bergeniae has the multiple medicinal functions such as hemostasis, antiinflammatory, cough-relieving, records " Rhizoma Seu Herba Bergeniae: reinforcement is strengthened, hemostasis, cough-relieving in " Traditional Chinese Medicine in Sichuan will ".It is weak that treating the liver is insufficiency of the spleen, over-strained hemoptysis, and internal injury is spat blood, pneumonopathy cough with asthma ".In addition, all beautiful physiology and appearances of the floral leaf due to Rhizoma Seu Herba Bergeniae self, are also widely used as ornamental plant.The present invention extracts the effective ingredient in Rhizoma Seu Herba Bergeniae root stock by experiment, and carried out cytotoxicity and cosmetics for skin activity experiment, specify that Rhizoma Seu Herba Bergeniae extract not only has good safety and also shows outstanding dual cosmetics for skin activity and good DPPH free radical scavenging effect and good tyrosinase activity inhibition, make it to become good natural skin care product raw material sources, development and utilization for Rhizoma Seu Herba Bergeniae provides better, better approach, and the sustainable use for Rhizoma Seu Herba Bergeniae future is significant.
Summary of the invention
The object of the invention is to provide Rhizoma Seu Herba Bergeniae extract, the cosmetics being active component with it, their preparation method, and is preparing the application in cosmetics.The present invention specify that Rhizoma Seu Herba Bergeniae extract has good skin effect and safety, becomes good natural skin care cosmetic raw materials.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
Rhizoma Seu Herba Bergeniae extract, is prepared by following method and obtains: getting Rhizoma Seu Herba Bergeniae root stock, pulverize, with the 85%-95% ethanol of the 15-30 times amount of Rhizoma Seu Herba Bergeniae, merceration 18-28h, then collects filtrate with buchner funnel, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 80-95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract.
Cosmetics, be made up of described Rhizoma Seu Herba Bergeniae extract and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
Described Rhizoma Seu Herba Bergeniae extract is preparing the application in cosmetics.
Described Rhizoma Seu Herba Bergeniae extract is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in whitening or antioxidative cosmetics, and is preparing the application improved in the cosmetics of skin metabolism function or skin barrier function.
Described Rhizoma Seu Herba Bergeniae extract is preparing the application in anesthetis, antifungal, antiinflammatory, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
Described Rhizoma Seu Herba Bergeniae extract is being prepared tyrosinase activity inhibitor and is preparing the application in antioxidant.
The present invention provides prevention simultaneously or treats dermopathic pharmaceutical composition, is made up of described Rhizoma Seu Herba Bergeniae extract and medicine common carrier.
And, the application that described Rhizoma Seu Herba Bergeniae extract prevents in preparation or treats in dermopathic medicine.
Present invention also offers the preparation method of Rhizoma Seu Herba Bergeniae extract, the method comprises the steps: to get Rhizoma Seu Herba Bergeniae root stock, pulverize, with the 85%-95% ethanol of the 15-30 times amount of Rhizoma Seu Herba Bergeniae, merceration 18-28h, then collects filtrate with buchner funnel, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 80-95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract.
Compared with prior art, the present invention has possessed following excellent benefit: the present invention obtains Rhizoma Seu Herba Bergeniae extract by carrying out concentration extraction to Rhizoma Seu Herba Bergeniae plant, cytotoxicity experiment and cosmetics for skin activity experiment have been carried out to Rhizoma Seu Herba Bergeniae extract, demonstrate the safety of Objective extraction thing, specify that the cosmetic active that Objective extraction thing is excellent.The invention provides a kind of better use pattern newly of Rhizoma Seu Herba Bergeniae, expand the source of natural skin care product plant material simultaneously.
Accompanying drawing illustrates:
Fig. 1: Rhizoma Seu Herba Bergeniae is to the scavenging capacity of DPPH free radical;
Fig. 2: Rhizoma Seu Herba Bergeniae extract is to the inhibitory action of tryrosinase;
Fig. 3: preparation technology's flow chart of Rhizoma Seu Herba Bergeniae extract.
Detailed description of the invention:
Below in conjunction with accompanying drawing, with embodiments of the invention, the present invention is more described in detail, but do not limit the scope of the invention.
Embodiment 1:
The preparation of Rhizoma Seu Herba Bergeniae extract:
Take Rhizoma Seu Herba Bergeniae sample (Bergeniapurpurascens (Hook.f.etThoms.) Engl.) (root stock) 16g, by sample grind into fine powder, with 85% ethanol 25 times amount (481-482mL) merceration 24h, then collect filtrate with buchner funnel, repeat 3 times.Filtrate is used Rotary Evaporators distilling under reduced pressure, concentrated filtrate, concentrated filtrate cold drying, obtains crude extract.By crude extract through macroporous resin (D101) adsorption bleaching, after 95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract 2.2g, extraction ratio is 13.75%.
Or, take Rhizoma Seu Herba Bergeniae sample (Bergeniapurpurascens (Hook.f.etThoms.) Engl.) (root stock) 16g, by sample grind into fine powder, with 95% ethanol 20 times amount merceration 20h, then collect filtrate with buchner funnel, repeat 4 times.Filtrate is used Rotary Evaporators distilling under reduced pressure, concentrated filtrate, concentrated filtrate cold drying, obtains crude extract.By crude extract through macroporous resin (D101) adsorption bleaching, after 90% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract 2.3g, extraction ratio is 14.38%.
Embodiment 2:
The cytotoxicity experiment of Rhizoma Seu Herba Bergeniae extract: by MTS colorimetry, determines the CC of sample
50(50%cytotoxicconcentration), namely to 50% cell produce toxicity time drug level, thus determine the secure sample concentration of following activity experiment.
Experimental technique: on 96 porocyte culture plates, mixes B16 cell with the drug solution to be measured of variable concentrations, sets 3 repeating holes, arranges the blank of not drug containing, 37 DEG C, 5%CO simultaneously
2cultivate 24h, adopt MTS colorimetric determination cytotoxicity, microplate reader measures OD value, measures wavelength 490nm.Calculate CC
50value.
Cell survival rate (%)=experimental port OD
490nm/ blank well OD
490nm× 100%
Experimental result: the cell survival rate of Flos Carthami inflorescence extract is 99.532%.Can obviously find out, after cell culture 24h, cell quantity does not almost change, and illustrate that Rhizoma Seu Herba Bergeniae extract does not have toxic action to cell, the safety as the natural material of cosmetics for skin is guaranteed.
Embodiment 2:
DPPH free radical scavenging is tested: by medicine to be measured and DPPH (final concentration is 100 μMs) hybrid reaction, set 3 repeating holes, arrange blank and the Trolox positive control of not drug containing simultaneously, 30 DEG C, 1h, microplate reader measures OD value, and determined wavelength is 515nm.Calculate antioxygen rate.
Antioxygen rate (%)=(1-experimental port OD
515nm/ blank well OD
515nm) × 100%
Experimental result: in an experiment, Rhizoma Seu Herba Bergeniae extract shows significant DPPH radical scavenging activity, and according to Concentraton gradient, present good dose-effect relationship (see Fig. 1), the good natural vegetation source possessing crease-resistant or sun-proof potentiality cosmetic active can be used as.
Embodiment 3:
Tryrosinase Inhibition test: medicine to be measured is mixed with L-Dopa (final concentration 1.25mM), add tryrosinase (final concentration 25U/mL) and start reaction, set 3 repeating holes, blank and the KojicAcid positive control of not drug containing are set simultaneously, room temperature, 5min, microplate reader measures OD value, and determined wavelength is 490nm.Calculate inhibitory activity against tyrosinase.
Inhibitory activity against tyrosinase (%)=(1 – (sample OD
490nm-sample Background control OD
490nm)/experiment contrast hole OD
490nm) × 100%
Experimental result: in control experiment, Rhizoma Seu Herba Bergeniae extract (IC
50=49.302 μ g/mL) show the inhibition (see Fig. 2) good to tryrosinase.The active application potential of the cosmetics for skin of the tool whitening of Rhizoma Seu Herba Bergeniae extract or de-black pigment (speckle) is described, can by as whitening or the good vegetation raw material of de-black pigment natural cosmetics.
The invention provides the topical composition with Rhizoma Seu Herba Bergeniae extract or Rhizoma Seu Herba Bergeniae extract mixtures, its acceptable carrier pharmaceutically or on cosmetics.These compositionss can suppress the tyrosinase activity of skin, and B16 cell melanin generates inhibit activities, and elastin laminin is active, and TNF-α secretes inhibit activities, and short collagen secretion is active, suppresses the oxidation of skin.These compositionss can be used for microgroove and/wrinkle, skin erythema, dryness, responsive skin, skin pruritus, colour of skin inequality or skin black speck, chafing and the uncomfortable disease of other skin related to this.These compositionss include the extract obtained from Rhizoma Seu Herba Bergeniae or the active component obtained from this plant.These compositionss can suppress the lipoxidase of skin further, and COX (Cycloxygenase) is active, MMP-1 and MMP-3 is active.In these compositionss, the content of Rhizoma Seu Herba Bergeniae extract is the 0.001wt%-20.0wt% of compositions gross weight.These compositionss can be skin care liquid, protective skin cream or Emulsion etc.The extract of Rhizoma Seu Herba Bergeniae can be water solubility extract, and this extract can use alcohol (such as methanol, ethanol, propanol, butanols etc.) to extract.
Compositions containing Rhizoma Seu Herba Bergeniae extract in the present invention can be used for topical skin care or hair products.These compositionss can make emulsifying agent (such as oil-in-water, Water-In-Oil, silicon Water-In-Oil, water bag silicone oil, W/O/W, Water-In-Oil bag oil, Water-In-Oil bag silicone oil etc.), cream, skin care liquid, liquid (such as water preparation or water-alcohol solution), anhydrous substrate (such as lip pomade or powder etc.), gel, ointment, milk, ointment, spray, solid formulation, eye cream etc.These compositionss of local application can alleviate the outward appearance (as skin erythema, allergic skin, red swelling of the skin, inflammation etc.) of skin erythema, also can alleviate the pain because erythema causes.Erythema generally can by the electrotherapy of skin sunburn, skin, skin burn, Contact hyper sensitization, systemic anaphylaxis, dermal toxicity, exercise, insect bite, bacteriological infection, viral infection, fungal infection, protozoa infection, massage and blow wind-induced dermatitis etc.The outward appearance of dry skin, sensitive skin or skin itching or reduction colour of skin inequality can be treated.The outward appearance of microgroove or wrinkle can be reduced.These extracts can be water miscible or non-water-soluble extracts, and their available alcohol (methanol, ethanol, propanol or butanols etc.), glycol, oil, water etc. extract.
Topical skin care compositions in the present invention includes the extract of Rhizoma Seu Herba Bergeniae or the component obtained from Rhizoma Seu Herba Bergeniae extract or composition.These compositionss of local application can be treated or prevent dermatosis.Dermatosis comprises dry skin, skin itching, chafing, erythema, skin allergy, pruritus, Aranea blood vessel, passeris montani saturati speckle, senile plaque, senile purpura, seborrheic keratosis, melasma, pimple, microgroove or wrinkle, tuberosity, skin sun-damaged, dermatitis (seborrheic dermatitis, nummular dermatitis, contact dermatitis, atoipc dermatitis, exfoliative dermatitis, Perioral Dermatitis and stasis dermatitis etc.), psoriasis, folliculitis, acne erythematosa, acne, pus born of the same parents are sick, erysipelas, erythrasma, eczema, sunburn, skin burn, open wound, infective factors etc.Dermatosis also can by exposing under ultraviolet light, age, radiation, be chronically exposed to the sun under, environmental pollution, air pollution, wind, hot and cold, chemicals, pathology, smoking or lack nutrition etc. and cause.This method increases the conversion rate of keratodermatitis by local application compositions of the present invention, increase the synthesis of collagen protein in fibroblast, strengthen cellular anti-oxidant defense mechanism (can improve as outer antioxidative strengthens, supplement or prevent cellular antioxidants as Skin Cell---keratinocyte, melanocyte, reduce in Langerhans cell or prevent skin, cell, the catalyzing enzyme of albumen and lipid oxidation damage and the loss of glutathion), melanic generation in check melanin cell, reduce or stop skin oxidative damage (comprising the oxidation of albumen in the quantity and/or skin reducing lipid peroxidation enzyme).
Compositions in the present invention can reduce the quantity of internal oxidation and/or the damage of external oxygen voltinism in cell, increases the synthesis of collagen protein in cell, reduces the generation of the infection cell factor in cell.These cells comprise human epidermic keratinocyte, fibroblasts of adult human dermis, or human melanoma cell, or their combination in any (as Human keratinocytes and the combination of human fibroblasts or the combination of people's keratinocyte and human melanoma cell).
Compositions in the present invention can treat hyperpigmentation, and this method also can comprise determines that a people is excessively calm the need for the treatment of skin pigment.Also can be used for reducing senile plaque, hypopigmentation of skin, the freckle in skin appearance by the compositions used in the present invention, reduce or prevent microgroove or the wrinkle of skin, increase the tightness of skin.
There is containing the compositions of Rhizoma Seu Herba Bergeniae extract in the present invention the ability of the activity of antioxidation, restraint of tyrosinase, suppression Elastase, suppression TNF-α enzyme, suppression lipoxidase, suppression COX-1 and/or COX-2 enzyme and suppression MMP-1 and/or MMP-2.These compositionss can be used as topical skin composition, comprise acceptable carrier on Dermatology.The dermatosis relevant to the activity of tryrosinase, Elastase, TNF-α enzyme, lipoxidase, COX-1 and/or COX-2 enzyme and MMP-1 and/or MMP-2 in skin oxidative, Skin Cell can be treated or prevent to these compositionss.
Embodiment 4:
Obtain Rhizoma Seu Herba Bergeniae extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:1, pelletizing press sheet.
Embodiment 5:
Obtain Rhizoma Seu Herba Bergeniae extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:2, pelletizing press sheet.
Embodiment 6:
Obtain Rhizoma Seu Herba Bergeniae extract by embodiment 1, capsule preparations method makes capsule routinely.
Embodiment 7:
First obtain Rhizoma Seu Herba Bergeniae extract by embodiment 1, then make tablet as follows
Tablet: Rhizoma Seu Herba Bergeniae extract 100mg
Starch is appropriate
Semen Maydis pulp is appropriate
Magnesium Stearate proper quantity
Embodiment 8:
Capsule: Rhizoma Seu Herba Bergeniae extract 100mg
Starch is appropriate
Magnesium Stearate proper quantity
Preparation method: mixed with auxiliary agent by Rhizoma Seu Herba Bergeniae extract, sieve, Homogeneous phase mixing in suitable container, loads hard gelatin capsule the mixture obtained.
Embodiment 9:
Preparation method: at every turn add a kind of composition under stirring in the double distilled water of proper volume, separates until completely dark, and then adds another kind of composition.After adding water to 2ml, this solution is filtered on sterilizing filter, to load in bottle and to separate according to suitable dosage.
Embodiment 10:
Drop pill: Rhizoma Seu Herba Bergeniae extract 1g
Polyethylene glycol 6000 9g
Method for making: the preparation of Rhizoma Seu Herba Bergeniae extract 6000 fused solution: take Rhizoma Seu Herba Bergeniae extract by above-mentioned recipe quantity, add appropriate dehydrated alcohol, after slight fever is dissolved, add (60 DEG C of water bath heat preservations) in the Polyethylene Glycol fused solution of recipe quantity, be uniformly mixed, till ethanol is waved to the greatest extent, be statically placed in 60 DEG C of water-baths and be incubated 30 minutes, treat that bubble eliminates, then the above-mentioned mixing fused solution eliminating bubble is proceeded in surge drum, under the condition of insulation 80-85 DEG C, control to drip speed, instill dropwise in condensed fluid, complete Deng condensation, incline condensed fluid, collect drop pill, drop is clean and remove the condensed fluid on ball with filter paper, place in silica gel drier or natural drying.
Embodiment 11:
Tablet: embodiment 1 gained Rhizoma Seu Herba Bergeniae extract 10mg, lactose 180mg, starch 55mg, magnesium stearate 5mg;
Preparation method: by Rhizoma Seu Herba Bergeniae extract, the mixing of newborn sugar and starch, evenly moistening with water, sieves the mixture after moistening drying, after sieve, adds magnesium stearate, and then by mixture tabletting, the heavy 250mg of every sheet, compounds content is 10mg.
Embodiment 12:
Ampulla: embodiment 1 gained Rhizoma Seu Herba Bergeniae extract 2mg, sodium chloride 10mg;
Preparation method: Rhizoma Seu Herba Bergeniae extract and sodium chloride are dissolved in appropriate water for injection, filters gained solution, aseptically loads in ampoule bottle.
Embodiment 13:
Capsule: embodiment 1 gained Rhizoma Seu Herba Bergeniae extract 10mg, lactose 187mg, magnesium stearate 3mg;
Preparation method: mixed with auxiliary agent by Rhizoma Seu Herba Bergeniae extract, sieve, Homogeneous phase mixing, loads hard gelatin capsule the mixture obtained, and the heavy 200mg of each capsule, active component content is 10mg.
Embodiment 14:
Skin whitener formula (W%) containing Rhizoma Seu Herba Bergeniae extract:
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 15:
Emulsion formulations (W%) containing Rhizoma Seu Herba Bergeniae extract:
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 16:
Formula:
A oil phase w (mass percent)
Cera Flava 2%, stearic acid 5%, octadecanol 3%, lanoline 2%, sorbester p17 3%, POLYSORBATE 80 3%, paraffin oil 3%.
B aqueous phase w (mass percent)
Glycerol 5%, Rhizoma Seu Herba Bergeniae 5% of the present invention, triethanolamine 0.3%, distilled water 75%.
C essence is appropriate, propylparaben 0.2%
Method:
Oil phase is placed in thermostat water bath be heated with stirring to 90 DEG C be fused into homogeneous phase completely after, slowly add aqueous phase, continue stir 30min; Add essence, propylparaben during slow cooling to 45 DEG C, continue to stir; When temperature is down to about 35 DEG C, stop stirring, leave standstill, cooling, obtains newborn creamy product.
Claims (9)
1. Rhizoma Seu Herba Bergeniae extract, is prepared by following method and obtains: getting Rhizoma Seu Herba Bergeniae root stock, pulverize, with the 85%-95% ethanol of the 15-30 times amount of Rhizoma Seu Herba Bergeniae, merceration 18-28h, then collects filtrate with buchner funnel, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 80-95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract.
2. cosmetics, be made up of Rhizoma Seu Herba Bergeniae extract according to claim 1 and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
3. Rhizoma Seu Herba Bergeniae extract according to claim 1 is preparing the application in cosmetics.
4. Rhizoma Seu Herba Bergeniae extract according to claim 1 is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in whitening or antioxidative cosmetics, and is preparing the application improved in the cosmetics of skin metabolism function or skin barrier function.
5. Rhizoma Seu Herba Bergeniae extract according to claim 1 is preparing the application in anesthetis, antifungal, antiinflammatory, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
6. Rhizoma Seu Herba Bergeniae extract according to claim 1 is being prepared tyrosinase activity inhibitor and is preparing the application in antioxidant.
7. prevent or treat dermopathic pharmaceutical composition, being made up of Rhizoma Seu Herba Bergeniae extract according to claim 1 and medicine common carrier.
8. Rhizoma Seu Herba Bergeniae extract according to claim 1 is in the application preparing prevention or treat in dermopathic medicine.
9. the preparation method of Rhizoma Seu Herba Bergeniae extract, is characterized in that the method comprises the steps: to get Rhizoma Seu Herba Bergeniae root stock, pulverize, with the 85%-95% ethanol of the 15-30 times amount of Rhizoma Seu Herba Bergeniae, merceration 18-28h, then collects filtrate with buchner funnel, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 80-95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Rhizoma Seu Herba Bergeniae extract.
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