CN105194102A - Camellia sinensis var.assamica extract, preparation method thereof and application of Camellia sinensis var.assamica extract in cosmetics - Google Patents

Camellia sinensis var.assamica extract, preparation method thereof and application of Camellia sinensis var.assamica extract in cosmetics Download PDF

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Publication number
CN105194102A
CN105194102A CN201510727904.9A CN201510727904A CN105194102A CN 105194102 A CN105194102 A CN 105194102A CN 201510727904 A CN201510727904 A CN 201510727904A CN 105194102 A CN105194102 A CN 105194102A
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China
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daye tea
extract
tea extract
skin
assamica
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CN201510727904.9A
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Chinese (zh)
Inventor
杨立新
许刚
刘爱忠
裴盛基
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Kunming Institute of Botany of CAS
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Kunming Institute of Botany of CAS
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Priority to CN201510727904.9A priority Critical patent/CN105194102A/en
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Abstract

The invention discloses Camellia sinensis var.assamica extract, a cosmetic additive adopting the Camellia sinensis var.assamica extract for realizing skin whitening, moisturizing and antioxidant activity and an application of the Camellia sinensis var.assamica extract in cosmetics. The Camellia sinensis var.assamica extract is tea-seed oil of Camellia sinensis var.assamica, has good activity for promoting collagen secretion and is a good active agent for promoting collagen secretion. The Camellia sinensis var.assamica extract has good moistening and moisture preserving functions as well as a skin metabolism improvement function and a skin barrier function in the aspect of skin care of a human body. With the effective utilization of a main raw material of pu'er tea in China, namely the tea-seed oil of the Camellia sinensis var.assamica, the Camellia sinensis var.assamica extract with very high skin cosmetic activity is obtained, and the extract can be used for preparing a good collagen promoting active agent, has excellent functions of winkle resisting, skin whitening, sunscreen and the like and is a good raw material for herbal cosmetics.

Description

Daye tea extract and preparation method thereof and the application in cosmetics
Technical field
The invention belongs to cosmetics and medical art, be specifically related to extract natural active matter from a kind of China Folium camelliae assamicae primary raw material daye tea--daye tea extract, the cosmetics being active component with it or medicine, and the application of daye tea extract in the various skin cosmetics such as preparation moisturizing, skin care, crease-resistant, sun-proof, whitening, also have in preparation treatment or prevent in dermopathic medicine application.
Technical background,
Daye tea (Camelliasinensisvar.assamica) is the evergreen dungarunga of Theaceae Camellia or shrub.Be thick with leaves, leaf ellipse is main, the poly-point of blade tip or gradually point, and blade face is swelled, leaf color is green glossy, and blade is thick and soft, blade back of the body volume or slightly infolding, leaf margin microwave, have obvious trunk, branch part is obvious, is grown on the ground such as Pu'er, the Yunnan Province of China province west and south, Jinghong.
Daye tea is that Yunnan Province of China economizes one of distinctive seeds, because containing abundant tea polyphenols, aminoacid, caffeine in its blade, becomes one of indispensable raw material of Folium camelliae assamicae.The present invention extract by experiment daye tea spend in effective ingredient, and carried out cytotoxicity and cosmetics for skin activity experiment, specify that daye tea extract not only has good safety and also shows outstanding cosmetics for skin activity and good short collagen secretion activity, make it to become good natural skin care product raw material sources, development and utilization for daye tea provides better, better approach, and the sustainable use for daye tea future is significant.
Summary of the invention
The object of the invention is to provide daye tea extract, the cosmetics being active component with it, their preparation method, and is preparing the application in cosmetics.The present invention specify that daye tea extract has good skin effect and safety, becomes good natural skin care cosmetic raw materials.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
Daye tea extract, prepared by following method and obtain: getting daye tea seed, carry out screening and removing impurities, carry out after the cold refining of hydraulic cold and physical squeezing become crude oil under the condition of pressure 350Pma-450Pma and temperature 25 DEG C-35 DEG C, with pure washing, precipitation, filtration, dehydration, filtration, obtain daye tea seed oil and be daye tea extract.
Present invention also offers a kind of cosmetics, be made up of above-mentioned daye tea extract and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
And described daye tea extract is preparing the application in cosmetics.
Described daye tea extract is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in skin-lightening cosmetic.
Described daye tea extract is preparing the application in anesthetis, antifungal, antiinflammatory, antioxidant, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
Described daye tea extract is preparing the application in anesthetis, antifungal, antiinflammatory, antioxidant, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
The application of described daye tea extract in the short collagen secretion activator of preparation.
Present invention also offers a kind of prevention or treat dermopathic pharmaceutical composition, being made up of described daye tea extract and medicine common carrier.
The application that daye tea extract of the present invention prevents in preparation or treats in dermopathic medicine.
The present invention additionally provides the preparation method of daye tea extract simultaneously, comprise the steps: to get daye tea seed, carry out screening and removing impurities, at pressure 350Pma-450Pma, carry out after the cold refining of hydraulic cold and physical squeezing become crude oil under the condition that temperature is 25 DEG C-35 DEG C, with pure washing, precipitation, filtration, dehydration, filtration, obtain daye tea seed oil and be daye tea extract.
Compared with prior art, the present invention possesses following excellent benefit: the present invention obtains Objective extraction thing by carrying out concentration extraction to daye tea plant, cytotoxicity experiment and cosmetics for skin activity experiment have been carried out to Objective extraction thing, demonstrate the safety of Objective extraction thing, specify that the cosmetic active that Objective extraction thing is excellent and pharmaceutically active.What invention increases daye tea utilizes function and added value, has expanded again the source of natural skin care product plant material.The present invention extracts the activity extract of daye tea bark by experiment, and carried out cytotoxicity and cosmetics for skin activity experiment, specify that daye tea extract not only has good safety and also shows good antioxidant activity, make it to become good natural skin care product material of vegetable origin, breach the limitation of daye tea tradition Land use systems, widen the utilization ways of daye tea, the utilization of resources improving daye tea has been worth and is significant.
Accompanying drawing illustrates:
Fig. 1: daye tea extract is to the facilitation of HDFa collagen secretion;
Fig. 2: preparation technology's flow chart of daye tea extract.
Detailed description of the invention:
Below in conjunction with accompanying drawing, with embodiments of the invention, the present invention is more described in detail, but do not limit the scope of the invention.
Embodiment 1:
The preparation of daye tea extract:
Get daye tea seed 1000g, carry out screening and removing impurities, carry out after the cold refining of hydraulic cold and physical squeezing become crude oil under the condition of pressure 350Pma and temperature 30 DEG C, with pure washing, precipitation, filtration, dehydration, filtration, obtain daye tea seed oil and daye tea extract 172.4g, extraction ratio is 17.2%.
Or get daye tea seed 1000g, carry out screening and removing impurities, carry out after the cold refining of hydraulic cold and physical squeezing become crude oil under the condition of pressure 450Pma and temperature 25 DEG C, with pure washing, precipitation, filtration, dehydration, filtration, obtain daye tea seed oil and daye tea extract 175g, extraction ratio is 17.5%.
Embodiment 2:
The cytotoxicity experiment of daye tea extract: by MTS colorimetry, determines the CC of sample 50(50%cytotoxicconcentration), namely to 50% cell produce toxicity time drug level, thus determine the secure sample concentration of following activity experiment.
Experimental technique: on 96 porocyte culture plates, mixes B16 cell with the drug solution to be measured of variable concentrations, sets 3 repeating holes, arranges the blank of not drug containing, 37 DEG C, 5%CO simultaneously 2cultivate 24h, adopt MTS colorimetric determination cytotoxicity, microplate reader measures OD value, measures wavelength 490nm.Calculate CC 50value.
Cell survival rate (%)=experimental port OD 490nm/ blank well OD 490nm× 100%
Experimental result: the cell survival rate of daye tea ethanol extraction is 96.288%, belong to normal level, obviously can find out that daye tea extract does not have toxic action to cell, so daye tea extract is guaranteed as the safety of the natural material of cosmetics for skin.
Embodiment 3:
Short HDFa collagen secretion experiment:
On 12 porocyte culture plates, mixed by HDFa cell with the drug solution to be measured of variable concentrations, the blank arranging not drug containing is shone and TGF-β positive control.37 DEG C, 5%CO 2cultivate 3 days, collect cells and supernatant, be stored in-80 DEG C; Add MTS, adopt the OD value of MTS colorimetric determination 490nm; Detect the secretion of collagen protein by the method provided in collagen E LISA test kit, microplate reader measures OD value, and determined wavelength is 450nm.Calculate collagen secretion increment rate.
Cell survival rate (%)=experimental port OD 490nm/ blank well OD 490nm× 100%
Collagen secretion increment rate (%)=(experimental port OD 450nm/ cell survival rate/blank well OD 450 nm-1) × 100%
Experimental result: with the Dual culture of people fibroblast, daye tea extract (EC 50=53.030 μ g/mL) present the active and dose-effect relationship (see Fig. 1) that tool is very strong of good short collagen secretion, illustrates that daye tea extract has and well moisten or moisturizing and improve the cosmetics for skin activity application potential of skin own metabolism function or skin barrier function.
The invention provides the compositions with natural plant composition or natural plant composition mixture, its acceptable carrier pharmaceutically or on cosmetics.These compositionss can suppress the tyrosinase activity of skin, and B16 cell melanin generates inhibit activities, and elastin laminin is active, and TNF-α secretes inhibit activities, and short collagen secretion is active, suppresses the oxidation of skin.These compositionss can be used for microgroove and/wrinkle, skin erythema, dryness, responsive skin, skin pruritus, colour of skin inequality or skin black speck, chafing and the uncomfortable disease of other skin related to this.These compositionss include the extract obtained from daye tea or the active component obtained from this plant.These compositionss can suppress the lipoxidase of skin further, and COX (Cycloxygenase) is active, MMP-1 and MMP-3 is active.In these compositionss, the content of daye tea extract is the 0.001wt% to 20.0wt% of compositions gross weight.These compositionss can be skin care liquid, protective skin cream or Emulsion etc.The extract of daye tea can be water solubility extract, and this extract can use alcohol (such as methanol, ethanol, propanol, butanols etc.) to extract.
The compositions that the present invention contains daye tea extract can be used for topical skin care or hair products.These compositionss can make emulsifying agent (such as oil-in-water, Water-In-Oil, silicon Water-In-Oil, water bag silicone oil, W/O/W, Water-In-Oil bag oil, Water-In-Oil bag silicone oil etc.), cream, skin care liquid, liquid (such as water preparation or water-alcohol solution), anhydrous substrate (such as lip pomade or powder etc.), gel, ointment, milk, ointment, spray, solid formulation, eye cream etc.
Topical skin care compositions of the present invention includes the extract of daye tea or the component obtained from daye tea extract or composition.These compositionss of local application can alleviate the outward appearance (as skin erythema, allergic skin, red swelling of the skin, inflammation etc.) of skin erythema, also can alleviate the pain because erythema causes.Erythema generally can by the electrotherapy of skin sunburn, skin, skin burn, Contact hyper sensitization, systemic anaphylaxis, dermal toxicity, exercise, insect bite, bacteriological infection, viral infection, fungal infection, protozoa infection, massage and blow wind-induced dermatitis etc.; The outward appearance of dry skin, sensitive skin or skin itching or reduction colour of skin inequality can be treated; The outward appearance of microgroove or wrinkle can be reduced.These extracts can be water miscible or non-water-soluble extracts, and their available alcohol (methanol, ethanol, propanol or butanols etc.), glycol, oil, water etc. extract.
Topical skin care compositions in the present invention includes the extract of daye tea or the component obtained from daye tea extract or composition.These compositionss of local application can be treated or prevent dermatosis.Dermatosis comprises dry skin, skin itching, chafing, erythema, skin allergy, pruritus, Aranea blood vessel, passeris montani saturati speckle, senile plaque, senile purpura, seborrheic keratosis, melasma, pimple, microgroove or wrinkle, tuberosity, skin sun-damaged, dermatitis (seborrheic dermatitis, nummular dermatitis, contact dermatitis, atoipc dermatitis, exfoliative dermatitis, Perioral Dermatitis and stasis dermatitis etc.), psoriasis, folliculitis, acne erythematosa, acne, pus born of the same parents are sick, erysipelas, erythrasma, eczema, sunburn, skin burn, open wound, infective factors etc.Dermatosis also can by exposing under ultraviolet light, age, radiation, be chronically exposed to the sun under, environmental pollution, air pollution, wind, hot and cold, chemicals, pathology, smoking or lack nutrition etc. and cause.This method increases the conversion rate of keratodermatitis by local application compositions of the present invention, increase the synthesis of collagen protein in fibroblast, strengthen cellular anti-oxidant defense mechanism (can improve as outer antioxidative strengthens, supplement or prevent cellular antioxidants as Skin Cell---keratinocyte, melanocyte, reduce in Langerhans cell or prevent skin, cell, the catalyzing enzyme of albumen and lipid oxidation damage and the loss of glutathion), melanic generation in check melanin cell, reduce or stop skin oxidative damage (comprising the oxidation of albumen in the quantity and/or skin reducing lipid peroxidation enzyme).
The compositions that the present invention contains daye tea extract can reduce the quantity of internal oxidation and/or the damage of external oxygen voltinism in cell, increases the synthesis of collagen protein in cell, reduces the generation of the infection cell factor in cell.These cells comprise human epidermic keratinocyte, fibroblasts of adult human dermis, or human melanoma cell, or their combination in any (as Human keratinocytes and the combination of human fibroblasts or the combination of people's keratinocyte and human melanoma cell).
The compositions that the present invention contains daye tea extract can treat hyperpigmentation, and this method also can comprise determines that a people is excessively calm the need for the treatment of skin pigment.Also can be used for reducing senile plaque, hypopigmentation of skin, the freckle in skin appearance by the compositions used in the present invention, reduce or prevent microgroove or the wrinkle of skin, increase the tightness of skin.
There is containing the compositions of daye tea extract in the present invention the ability of the activity of antioxidation, restraint of tyrosinase, suppression Elastase, suppression TNF-α enzyme, suppression lipoxidase, suppression COX-1 and/or COX-2 enzyme and suppression MMP-1 and/or MMP-2.These compositionss can be used as topical skin composition, comprise acceptable carrier on Dermatology.The dermatosis relevant to the activity of tryrosinase, Elastase, TNF-α enzyme, lipoxidase, COX-1 and/or COX-2 enzyme and MMP-1 and/or MMP-2 in skin oxidative, Skin Cell can be treated or prevent to these compositionss.
Embodiment 4:
Obtain daye tea extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:1, pelletizing press sheet.
Embodiment 5:
Obtain daye tea activity extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:2, pelletizing press sheet.
Embodiment 6:
Obtain daye tea extract by embodiment 1, capsule preparations method makes capsule routinely.
Embodiment 7:
First obtain daye tea extract by embodiment 1, then make tablet as follows
Tablet: daye tea extract 100mg
Starch is appropriate
Semen Maydis pulp is appropriate
Magnesium Stearate proper quantity
Embodiment 8:
Capsule: daye tea extract 100mg
Starch is appropriate
Magnesium Stearate proper quantity
Preparation method: mixed with auxiliary agent by red daye tea extract, sieve, Homogeneous phase mixing in suitable container, loads hard gelatin capsule the mixture obtained.
Embodiment 9:
Preparation method: at every turn add a kind of composition under stirring in the double distilled water of proper volume, separates until completely dark, and then adds another kind of composition.After adding water to 2ml, this solution is filtered on sterilizing filter, to load in bottle and to separate according to suitable dosage.
Embodiment 10:
Drop pill: daye tea extract 1g
Polyethylene glycol 6000 9g
Method for making: the preparation of daye tea extract 6000 fused solution: take daye tea extract by above-mentioned recipe quantity, add appropriate dehydrated alcohol, after slight fever is dissolved, add (60 DEG C of water bath heat preservations) in the Polyethylene Glycol fused solution of recipe quantity, be uniformly mixed, till ethanol is waved to the greatest extent, be statically placed in 60 DEG C of water-baths and be incubated 30 minutes, treat that bubble eliminates, then the above-mentioned mixing fused solution eliminating bubble is proceeded in surge drum, under the condition of insulation 80-85 DEG C, control to drip speed, instill dropwise in condensed fluid, complete Deng condensation, incline condensed fluid, collect drop pill, drop is clean and remove the condensed fluid on ball with filter paper, place in silica gel drier or natural drying.
Embodiment 11:
Tablet: embodiment 1 gained daye tea extract 10mg, lactose 180mg, starch 55mg, magnesium stearate 5mg;
Preparation method: by extract, the mixing of newborn sugar and starch, evenly moistening with water, sieves the mixture after moistening drying, after sieve, adds magnesium stearate, and then by mixture tabletting, the heavy 250mg of every sheet, compounds content is 10mg.
Embodiment 12:
Ampulla: embodiment 1 gained daye tea extract 2mg, sodium chloride 10mg;
Preparation method: daye tea extract and sodium chloride are dissolved in appropriate water for injection, filters gained solution, aseptically loads in ampoule bottle.
Embodiment 13:
Capsule: embodiment 1 gained daye tea extract 10mg, lactose 187mg, magnesium stearate 3mg;
Preparation method: mixed with auxiliary agent by daye tea extract, sieve, Homogeneous phase mixing, loads hard gelatin capsule the mixture obtained, and the heavy 200mg of each capsule, active component content is 10mg.
Embodiment 14:
Skin whitener formula (W%) containing daye tea extract:
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 15:
Emulsion formulations (W%) containing daye tea extract:
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 16:
Formula:
A oil phase w (mass percent)
Cera Flava 2%, stearic acid 5%, octadecanol 3%, lanoline 2%, sorbester p17 3%, POLYSORBATE 80 3%,
Paraffin oil 3%.
B aqueous phase w (mass percent)
Glycerol 5%, daye tea extract 5% of the present invention, triethanolamine 0.3%, distilled water 75%.
C essence is appropriate, propylparaben 0.2%
Method:
Oil phase is placed in thermostat water bath be heated with stirring to 90 DEG C be fused into homogeneous phase completely after, slowly add aqueous phase, continue stir 30min; Add essence, propylparaben during slow cooling to 45 DEG C, continue to stir; When temperature is down to about 35 DEG C, stop stirring, leave standstill, cooling, obtains newborn creamy product.

Claims (9)

1. daye tea extract, prepared by following method and obtain: getting daye tea seed, carry out screening and removing impurities, at pressure 350Pma-450Pma, carry out after the cold refining of hydraulic cold and physical squeezing become crude oil under the condition that temperature is 25 DEG C-35 DEG C, with pure washing, precipitation, filtration, dehydration, filtration, obtain daye tea seed oil and be daye tea extract.
2. cosmetics, be made up of daye tea extract according to claim 1 and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
3. daye tea extract according to claim 1 is preparing the application in cosmetics.
4. daye tea extract according to claim 1 is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in skin-lightening cosmetic.
5. daye tea extract according to claim 1 is preparing the application in anesthetis, antifungal, antiinflammatory, antioxidant, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
6. the application of daye tea extract according to claim 1 in the short collagen secretion activating agent of preparation.
7. prevent or treat dermopathic pharmaceutical composition, being made up of daye tea extract according to claim 1 and medicine common carrier.
8. daye tea extract according to claim 1 is in the application preparing prevention or treat in dermopathic medicine.
9. the preparation method of daye tea extract, comprises the steps: to get daye tea seed, carries out screening and removing impurities, at pressure 350Pma-450Pma, carries out hydraulic cold under the condition that temperature is 25 DEG C-35 DEG C, obtains daye tea seed oil.
CN201510727904.9A 2015-10-30 2015-10-30 Camellia sinensis var.assamica extract, preparation method thereof and application of Camellia sinensis var.assamica extract in cosmetics Pending CN105194102A (en)

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Application publication date: 20151230