CN105193675A - Docynia delavayi(Franch.)Schneid. extract as well as preparation method and application of (Docynia delavayi(Franch.)Schneid. in preparation of cosmetics - Google Patents
Docynia delavayi(Franch.)Schneid. extract as well as preparation method and application of (Docynia delavayi(Franch.)Schneid. in preparation of cosmetics Download PDFInfo
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- CN105193675A CN105193675A CN201510724427.0A CN201510724427A CN105193675A CN 105193675 A CN105193675 A CN 105193675A CN 201510724427 A CN201510724427 A CN 201510724427A CN 105193675 A CN105193675 A CN 105193675A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0043—Nose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2013—Organic compounds, e.g. phospholipids, fats
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/2031—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyethylene oxide, poloxamers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4841—Filling excipients; Inactive ingredients
- A61K9/4858—Organic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses Docynia delavayi(Franch.)Schneid. plant extract as well as a combined application of Docynia delavayi(Franch.)Schneid. serving as a cosmetic additive with functions of skin whitening and moistening and with antioxidant activity in preparation of cosmetics. Docynia delavayi(Franch.)Schneid. extract is water extract or ethanol extract of Docynia delavayi(Franch.)Schneid., has good DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity and is a good natural antioxidant. In terms of human skin care, the Docynia delavayi(Franch.)Schneid. extract has excellent potential in application of skin cosmetics with anti-wrinkling, whitening and sun protection functions and the like. The medicinal plant from a Chinese traditional minority, Docynia delavayi(Franch.)Schneid. is effectively used for preparation of the Docynia delavayi(Franch.)Schneid. extract with very high activity for skin cosmetics, the extract can be used for preparation of cosmetics, is a good antioxidant, has excellent anti-wrinkling, whitening, sun protection functions and the like and is a good natural plant cosmetic raw material.
Description
Technical field
The invention belongs to cosmetics and medical art, be specifically related to extract natural active matter the traditional medicinal plants Yunnan Docynia delavayi (Franch.) Schneid. commonly used from a kind of Minorities In China area--Yunnan Docynia delavayi (Franch.) Schneid. extract, the cosmetics being active component with it or medicine, and the application of Yunnan Docynia delavayi (Franch.) Schneid. extract in the various skin cosmetics such as preparation moisturizing, skin care, crease-resistant, sun-proof, whitening, also have in preparation treatment or prevent in dermopathic medicine application
Technical background,
Yunnan Docynia delavayi (Franch.) Schneid. (Docyniadelavayi (Franch.) Schneid.), for Rosaceae Yi Yi belongs to aiphyllium.Sprig is sturdy, has yellow-white fine hair, gradually comes off, bronzing or puce during children; Blade lanceolar or ovum shape lanceolar, melonidum is comparatively large, avette or square is circular.Be grown in hill limes marginis, small stream side or shrubbery, be common in the ground such as Yunnan Province of China, Sichuan, Guizhou.
Yunnan Docynia delavayi (Franch.) Schneid. is one of regional conventional traditional medicinal plants of Chinese Minority Nationalities, its bark and leaves collection is brewed into cream and can effectively treats the wounds such as burn, scald, fracture.In addition, owing to being rich in organic acid in Docynia delavayi (Franch.) Schneid. fruit, and mouthfeel is unique, so the fruit that Docynia delavayi (Franch.) Schneid. fruit is ethnic groups generally to be had deep love for.But, the compositions of Yunnan Docynia delavayi (Franch.) Schneid. or its extract and extract thereof is used for have not been reported in cosmetics for skin.
Summary of the invention
The object of the invention is to provide Yunnan Docynia delavayi (Franch.) Schneid. extract, the cosmetics being active component with it, their preparation method, and is preparing the application in cosmetics.The present invention specify that Yunnan Docynia delavayi (Franch.) Schneid. extract has good skin effect and safety, becomes good natural skin care cosmetic raw materials.
In order to realize above-mentioned purpose of the present invention, the invention provides following technical scheme:
Yunnan Docynia delavayi (Franch.) Schneid. extract, is prepared by following method and obtains: getting Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, with the 85%-95% ethanol of Yunnan Docynia delavayi (Franch.) Schneid. bark 15-30 times amount, merceration 18-28h, collects filtrate, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 85%-95% ethanol elution, concentrated evaporate to dryness, frozen drying, obtains Yunnan Docynia delavayi (Franch.) Schneid. alcohol extract; Or get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, wash, carry out screening and removing impurities, obtain crude extract, then with little fiery infusion crude extract 8-15h, collect filtrate, concentrated evaporate to dryness obtains Yunnan Docynia delavayi (Franch.) Schneid. bark water extract.
Invention also provides the preparation method of above-mentioned Yunnan Docynia delavayi (Franch.) Schneid. extract, comprise the steps: to get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, with the 85%-95% ethanol of Yunnan Docynia delavayi (Franch.) Schneid. bark 15-30 times amount, merceration 18-28h, collects filtrate, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 85%-95% ethanol elution, concentrated evaporate to dryness, frozen drying, obtains Yunnan Docynia delavayi (Franch.) Schneid. bark ethanol extraction; Or get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, wash, carry out screening and removing impurities, obtain crude extract, then with little fiery infusion crude extract 8-15h, collect filtrate, concentrated evaporate to dryness obtains Yunnan Docynia delavayi (Franch.) Schneid. bark water extract.
Present invention also offers a kind of cosmetics, be made up of above-mentioned Yunnan Docynia delavayi (Franch.) Schneid. extract and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
And described Yunnan Docynia delavayi (Franch.) Schneid. extract is preparing the application in cosmetics.
Described Yunnan Docynia delavayi (Franch.) Schneid. extract is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in skin-lightening cosmetic.
Described Yunnan Docynia delavayi (Franch.) Schneid. extract is preparing the application in anesthetis, antifungal, antiinflammatory, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
Described Yunnan Docynia delavayi (Franch.) Schneid. extract is preparing the application in anesthetis, antifungal, antiinflammatory, antioxidant, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin.
Described Yunnan Docynia delavayi (Franch.) Schneid. extract is preparing the application in antioxidant.
Present invention also offers a kind of prevention or treat dermopathic pharmaceutical composition, being made up of described Yunnan Docynia delavayi (Franch.) Schneid. extract and medicine common carrier.
The application that Yunnan of the present invention Docynia delavayi (Franch.) Schneid. extract prevents in preparation or treats in dermopathic medicine.
The invention provides the topical composition with natural plant composition or natural plant composition mixture, its acceptable carrier pharmaceutically or on cosmetics.These compositionss can suppress the tyrosinase activity of skin, and B16 cell melanin generates inhibit activities, and elastin laminin is active, and TNF-α secretes inhibit activities, and short collagen secretion is active, suppresses the oxidation of skin.These compositionss can be used for microgroove and/wrinkle, skin erythema, dryness, responsive skin, skin pruritus, colour of skin inequality or skin black speck, chafing and the uncomfortable disease of other skin related to this.These compositionss include the extract obtained from the Docynia delavayi (Franch.) Schneid. of Yunnan or the active component obtained from this plant.These compositionss can suppress the lipoxidase of skin further, and COX (Cycloxygenase) is active, MMP-1 and MMP-3 is active.In these compositionss, the content of Yunnan Docynia delavayi (Franch.) Schneid. extract is the 0.001wt%-20.0wt% of compositions gross weight.These compositionss can be skin care liquid, protective skin cream or Emulsion etc.The extract of Yunnan Docynia delavayi (Franch.) Schneid. can be water solubility extract, and this extract can use alcohol (such as methanol, ethanol, propanol, butanols etc.) to extract.
Compositions containing Yunnan Docynia delavayi (Franch.) Schneid. extract in the present invention can be used for topical skin care or hair products.These compositionss can make emulsifying agent (such as oil-in-water, Water-In-Oil, silicon Water-In-Oil, water bag silicone oil, W/O/W, Water-In-Oil bag oil, Water-In-Oil bag silicone oil etc.), cream, skin care liquid, liquid (such as water preparation or water-alcohol solution), anhydrous substrate (such as lip pomade or powder etc.), gel, ointment, milk, ointment, spray, solid formulation, eye cream etc.
Topical skin care compositions in the present invention includes the extract of Yunnan Docynia delavayi (Franch.) Schneid. or the component obtained from the Docynia delavayi (Franch.) Schneid. extract of Yunnan or composition.These compositionss of local application can alleviate the outward appearance (as skin erythema, allergic skin, red swelling of the skin, inflammation etc.) of skin erythema, also can alleviate the pain because erythema causes.Erythema generally can by the electrotherapy of skin sunburn, skin, skin burn, Contact hyper sensitization, systemic anaphylaxis, dermal toxicity, exercise, insect bite, bacteriological infection, viral infection, fungal infection, protozoa infection, massage and blow wind-induced dermatitis etc.The outward appearance of dry skin, sensitive skin or skin itching or reduction colour of skin inequality can be treated.The outward appearance of microgroove or wrinkle can be reduced.These extracts can be water miscible or non-water-soluble extracts, and their available alcohol (methanol, ethanol, propanol or butanols etc.), glycol, oil, water etc. extract.
Topical skin care compositions of the present invention includes the extract of Yunnan Docynia delavayi (Franch.) Schneid. or the component obtained from the Docynia delavayi (Franch.) Schneid. extract of Yunnan or composition.These compositionss of local application can be treated or prevent dermatosis.Dermatosis comprises dry skin, skin itching, chafing, erythema, skin allergy, pruritus, Aranea blood vessel, passeris montani saturati speckle, senile plaque, senile purpura, seborrheic keratosis, melasma, pimple, microgroove or wrinkle, tuberosity, skin sun-damaged, dermatitis (seborrheic dermatitis, nummular dermatitis, contact dermatitis, atoipc dermatitis, exfoliative dermatitis, Perioral Dermatitis and stasis dermatitis etc.), psoriasis, folliculitis, acne erythematosa, acne, pus born of the same parents are sick, erysipelas, erythrasma, eczema, sunburn, skin burn, open wound, infective factors etc.Dermatosis also can by exposing under ultraviolet light, age, radiation, be chronically exposed to the sun under, environmental pollution, air pollution, wind, hot and cold, chemicals, pathology, smoking or lack nutrition etc. and cause.This method increases the conversion rate of keratodermatitis by local application compositions of the present invention, increase the synthesis of collagen protein in fibroblast, strengthen cellular anti-oxidant defense mechanism (can improve as outer antioxidative strengthens, supplement or prevent cellular antioxidants as Skin Cell---keratinocyte, melanocyte, reduce in Langerhans cell or prevent skin, cell, the catalyzing enzyme of albumen and lipid oxidation damage and the loss of glutathion), melanic generation in check melanin cell, reduce or stop skin oxidative damage (comprising the oxidation of albumen in the quantity and/or skin reducing lipid peroxidation enzyme).
Compositions of the present invention can reduce the quantity of internal oxidation and/or the damage of external oxygen voltinism in cell, increases the synthesis of collagen protein in cell, reduces the generation of the infection cell factor in cell.These cells comprise human epidermic keratinocyte, fibroblasts of adult human dermis, or human melanoma cell, or their combination in any (as Human keratinocytes and the combination of human fibroblasts or the combination of people's keratinocyte and human melanoma cell).Compositions of the present invention can treat hyperpigmentation, and this method also can comprise determines that a people is excessively calm the need for the treatment of skin pigment.Also can be used for reducing senile plaque, hypopigmentation of skin, the freckle in skin appearance by the compositions used in the present invention, reduce or prevent microgroove or the wrinkle of skin, increase the tightness of skin.
The present invention has antioxidation, restraint of tyrosinase, suppression Elastase containing the compositions of Yunnan Docynia delavayi (Franch.) Schneid. extract, suppress TNF-α enzyme, suppresses lipoxidase, suppresses COX-1 and/or COX-2 enzyme and suppresses the ability of activity of MMP-1 and/or MMP-2.These compositionss can be used as topical skin composition, comprise acceptable carrier on Dermatology.The dermatosis relevant to the activity of tryrosinase, Elastase, TNF-α enzyme, lipoxidase, COX-1 and/or COX-2 enzyme and MMP-1 and/or MMP-2 in skin oxidative, Skin Cell can be treated or prevent to these compositionss.
Compared with prior art, the present invention possesses following excellent benefit: the present invention obtains Objective extraction thing by carrying out concentration extraction to Yunnan Docynia delavayi (Franch.) Schneid. plant, cytotoxicity experiment and cosmetics for skin activity experiment have been carried out to Objective extraction thing, demonstrate the safety of Objective extraction thing, specify that the cosmetic active that Objective extraction thing is excellent and pharmaceutically active.What invention increases Yunnan Docynia delavayi (Franch.) Schneid. utilizes function and added value, has expanded again the source of natural skin care product plant material.The present invention extracts the activity extract of Yunnan Docynia delavayi (Franch.) Schneid. bark by experiment, and carried out cytotoxicity and cosmetics for skin activity experiment, specify that Yunnan Docynia delavayi (Franch.) Schneid. extract not only has good safety and also shows good antioxidant activity, make it to become good natural skin care product material of vegetable origin, breach the limitation of Yunnan Docynia delavayi (Franch.) Schneid. tradition Land use systems, widen the utilization ways of Yunnan Docynia delavayi (Franch.) Schneid., the utilization of resources improving Yunnan Docynia delavayi (Franch.) Schneid. has been worth and is significant.
Accompanying drawing illustrates:
Fig. 1: Yunnan Docynia delavayi (Franch.) Schneid. bark water extract is to the scavenging capacity of DPPH free radical;
Fig. 2: Yunnan Docynia delavayi (Franch.) Schneid. bark alcohol extract is to the scavenging capacity of DPPH free radical;
Fig. 3: preparation technology's flow chart of Yunnan Docynia delavayi (Franch.) Schneid. extract.
Detailed description of the invention:
Below in conjunction with accompanying drawing, with embodiments of the invention, the present invention is more described in detail, but do not limit the scope of the invention.
Embodiment 1:
The preparation of Yunnan Docynia delavayi (Franch.) Schneid. extract:
Take Yunnan Docynia delavayi (Franch.) Schneid. (Docyniadelavayi (Franch.) Schneid.) bark 15g, by sample grind into fine powder, with 85% ethanol 25 times amount (452mL) merceration 24h, then collect filtrate with buchner funnel, repeat 3 times.Filtrate is used Rotary Evaporators distilling under reduced pressure, concentrated filtrate, concentrated filtrate cold drying, obtains crude extract.By crude extract through macroporous resin (D101) adsorption bleaching, after 95% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Yunnan Docynia delavayi (Franch.) Schneid. bark alcohol extract 1.4g, extraction ratio is 9.3%.
Or, take Yunnan Docynia delavayi (Franch.) Schneid. (Docyniadelavayi (Franch.) Schneid.) bark 15g, by sample grind into fine powder, with 95% ethanol 20 times amount merceration 20h, then collect filtrate with buchner funnel, repeat 4 times.Filtrate is used Rotary Evaporators distilling under reduced pressure, concentrated filtrate, concentrated filtrate cold drying, obtains crude extract.By crude extract through macroporous resin (D101) adsorption bleaching, after 90% ethanol elution, eluent is concentrated evaporate to dryness, frozen drying, obtain Yunnan Docynia delavayi (Franch.) Schneid. bark alcohol extract 1.4g, extraction ratio is 9.3%.
Or get Yunnan Docynia delavayi (Franch.) Schneid. bark 100g, pulverize, wash, carry out screening and removing impurities, obtain crude extract, then with little fiery infusion crude extract 8h, collect filtrate, concentrated evaporate to dryness obtains Yunnan Docynia delavayi (Franch.) Schneid. bark water extract 13.6g, and extraction ratio is 13.6%.
Embodiment 2:
The cytotoxicity experiment of Yunnan Docynia delavayi (Franch.) Schneid. extract: by MTS colorimetry, determines the CC of sample
50(50%cytotoxicconcentration), namely to 50% cell produce toxicity time drug level, thus determine the secure sample concentration of following activity experiment.
Experimental technique: on 96 porocyte culture plates, mixes B16 cell with the drug solution to be measured of variable concentrations, sets 3 repeating holes, arranges the blank of not drug containing, 37 DEG C, 5%CO simultaneously
2cultivate 24h, adopt MTS colorimetric determination cytotoxicity, microplate reader measures OD value, measures wavelength 490nm.Calculate CC
50value.
Cell survival rate (%)=experimental port OD
490nm/ blank well OD
490nm× 100%
Experimental result: the cell survival rate of Yunnan Docynia delavayi (Franch.) Schneid. bark ethanol extraction is 87.255%, the cell survival rate of Yunnan Docynia delavayi (Franch.) Schneid. bark water extract is 96.200%, belong to normal level, obviously can find out that Yunnan Docynia delavayi (Franch.) Schneid. extract does not have toxic action to cell, the safety as the natural material of cosmetics for skin is guaranteed.
Embodiment 3:
DPPH free radical scavenging is tested: by medicine to be measured and DPPH (final concentration is 100 μMs) hybrid reaction, set 3 repeating holes, arrange blank and the Trolox positive control of not drug containing simultaneously, 30 DEG C, 1h, microplate reader measures OD value, and determined wavelength is 515nm.Calculate antioxygen rate.
Antioxygen rate (%)=(1-experimental port OD
515nm/ blank well OD
515nm) × 100%
Experimental result: in an experiment, Yunnan Docynia delavayi (Franch.) Schneid. bark extract all shows significant DPPH radical scavenging activity, and according to Concentraton gradient, present good dose-effect relationship (see Fig. 1), the good natural vegetation source possessing crease-resistant or sun-proof potentiality cosmetic active can be used as.
Embodiment 4:
Obtain Yunnan Docynia delavayi (Franch.) Schneid. extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:1, pelletizing press sheet.
Embodiment 5:
Obtain Yunnan Docynia delavayi (Franch.) Schneid. activity extract by embodiment 1, add excipient in extract and excipient weight than the ratio of 1:2, pelletizing press sheet.
Embodiment 6:
Obtain Yunnan Docynia delavayi (Franch.) Schneid. extract by embodiment 1, capsule preparations method makes capsule routinely.
Embodiment 7:
First obtain Yunnan Docynia delavayi (Franch.) Schneid. extract by embodiment 1, then make tablet as follows
Tablet: Yunnan Docynia delavayi (Franch.) Schneid. extract 100mg
Starch is appropriate
Semen Maydis pulp is appropriate
Magnesium Stearate proper quantity
Embodiment 8:
Capsule: Yunnan Docynia delavayi (Franch.) Schneid. extract 100mg
Starch is appropriate
Magnesium Stearate proper quantity
Preparation method: mixed with auxiliary agent by red Yunnan Docynia delavayi (Franch.) Schneid. extract, sieve, Homogeneous phase mixing in suitable container, loads hard gelatin capsule the mixture obtained.
Embodiment 9:
Preparation method: at every turn add a kind of composition under stirring in the double distilled water of proper volume, separates until completely dark, and then adds another kind of composition.After adding water to 2ml, this solution is filtered on sterilizing filter, to load in bottle and to separate according to suitable dosage.
Embodiment 10:
Drop pill: Yunnan Docynia delavayi (Franch.) Schneid. extract 1g
Polyethylene glycol 6000 9g
Method for making: the preparation of Yunnan Docynia delavayi (Franch.) Schneid. extract 6000 fused solution: take Yunnan Docynia delavayi (Franch.) Schneid. extract by above-mentioned recipe quantity, add appropriate dehydrated alcohol, after slight fever is dissolved, add (60 DEG C of water bath heat preservations) in the Polyethylene Glycol fused solution of recipe quantity, be uniformly mixed, till ethanol is waved to the greatest extent, be statically placed in 60 DEG C of water-baths and be incubated 30 minutes, treat that bubble eliminates, then the above-mentioned mixing fused solution eliminating bubble is proceeded in surge drum, under the condition of insulation 80-85 DEG C, control to drip speed, instill dropwise in condensed fluid, complete Deng condensation, incline condensed fluid, collect drop pill, drop is clean and remove the condensed fluid on ball with filter paper, place in silica gel drier or natural drying.
Embodiment 11:
Tablet: embodiment 1 gained Yunnan Docynia delavayi (Franch.) Schneid. extract 10mg, lactose 180mg, starch 55mg, magnesium stearate 5mg;
Preparation method: by extract, the mixing of newborn sugar and starch, evenly moistening with water, sieves the mixture after moistening drying, after sieve, adds magnesium stearate, and then by mixture tabletting, the heavy 250mg of every sheet, compounds content is 10mg.
Embodiment 12:
Ampulla: embodiment 1 gained Yunnan Docynia delavayi (Franch.) Schneid. extract 2mg, sodium chloride 10mg;
Preparation method: be dissolved in appropriate water for injection by Yunnan Docynia delavayi (Franch.) Schneid. extract and sodium chloride, filters gained solution, aseptically loads in ampoule bottle.
Embodiment 13:
Capsule: embodiment 1 gained Yunnan Docynia delavayi (Franch.) Schneid. extract 10mg, lactose 187mg, magnesium stearate 3mg;
Preparation method: mixed with auxiliary agent by Yunnan Docynia delavayi (Franch.) Schneid. extract, sieve, Homogeneous phase mixing, loads hard gelatin capsule the mixture obtained, and the heavy 200mg of each capsule, active component content is 10mg.
Embodiment 14:
Skin whitener formula (W%) containing Yunnan Docynia delavayi (Franch.) Schneid. extract:
Deionized water adds to 100
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 15:
Emulsion formulations (W%) containing Yunnan Docynia delavayi (Franch.) Schneid. extract:
Deionized water adds to 100
The method making cosmetics routinely obtains the cosmetics of the above-mentioned formula of the present invention.
Embodiment 16:
Formula:
A oil phase w (mass percent)
Cera Flava 2%, stearic acid 5%, octadecanol 3%, lanoline 2%, sorbester p17 3%, POLYSORBATE 80 3%, paraffin oil 3%.
B aqueous phase w (mass percent)
Glycerol 5%, Yunnan of the present invention Docynia delavayi (Franch.) Schneid. extract 5%, triethanolamine 0.3%, distilled water 75%.
C essence is appropriate, propylparaben 0.2%
Method:
Oil phase is placed in thermostat water bath be heated with stirring to 90 DEG C be fused into homogeneous phase completely after, slowly add aqueous phase, continue stir 30min; Add essence, propylparaben during slow cooling to 45 DEG C, continue to stir; When temperature is down to about 35 DEG C, stop stirring, leave standstill, cooling, obtains newborn creamy product.
Claims (9)
1. Yunnan Docynia delavayi (Franch.) Schneid. extract, is prepared by following method and obtains: getting Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, with 85% ethanol of Yunnan Docynia delavayi (Franch.) Schneid. bark 3-5 times of volume, merceration 18-28h, collects filtrate, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 85%-95% ethanol elution, concentrated evaporate to dryness, frozen drying, obtains Yunnan Docynia delavayi (Franch.) Schneid. bark alcohol extract; Or get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, wash, carry out screening and removing impurities, obtain crude extract, then with little fiery infusion crude extract 8-15h, collect filtrate, concentrated evaporate to dryness obtains Yunnan Docynia delavayi (Franch.) Schneid. bark water extract.
2. the preparation method of Yunnan Docynia delavayi (Franch.) Schneid. extract, is characterized in that the method comprises the steps: to get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, with the 85%-95% ethanol of Yunnan Docynia delavayi (Franch.) Schneid. bark 15-30 times amount, merceration 18-28h, collects filtrate, repeat 2-5 time, filtrate decompression is distilled, concentrated filtrate, cold drying, obtains crude extract, is decoloured by crude extract through macroporous resin adsorption, after 85%-95% ethanol elution, concentrated evaporate to dryness, frozen drying, obtains Yunnan Docynia delavayi (Franch.) Schneid. bark alcohol extract; Or get Yunnan Docynia delavayi (Franch.) Schneid. bark, pulverize, wash, carry out screening and removing impurities, obtain crude extract, then with little fiery infusion crude extract 8-15h, collect filtrate, concentrated evaporate to dryness obtains Yunnan Docynia delavayi (Franch.) Schneid. bark water extract.
3. cosmetics, be made up of Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. extract and cosmetics common carrier, described carrier is selected from one or more Aquo Systems, glycerol, C1-4 alcohol, fatty alcohol, polyhydric alcohol, aliphatic ether, fatty ester, ethylene glycol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water, or their combination.
4. the application in cosmetics prepared by Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. peel extract.
5. Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. peel extract is in preparation moisturizing or skin care is crease-resistant or sun-proof or application in skin-lightening cosmetic.
6. the application in anesthetis, antifungal, antiinflammatory, antiseptic, chelating agen, coloring agent, depigmenting agent, softening agent, emulsifying agent, agents for defoliating, film former, aromatic, wetting agent, anthelmintic, lubricant, humidizer, medicament, light stabilizer, antiseptic, Derma-Guard, skin penetration enhancer, sunscreen, stabilizing agent, surfactant, thickening agent, viscosity modifier, vitamin prepared by Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. peel extract.
7. the application in antioxidant prepared by Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. peel extract.
8. prevent or treat dermopathic pharmaceutical composition, being made up of Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. extract and medicine common carrier.
9. Yunnan according to claim 1 Docynia delavayi (Franch.) Schneid. peel extract is in the application preparing prevention or treat in dermopathic medicine.
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CN106038738A (en) * | 2016-04-18 | 2016-10-26 | 四川师范大学 | Docynia delavayi polyphenol extract and preparation method thereof |
CN111758576A (en) * | 2020-08-04 | 2020-10-13 | 西南林业大学 | Rapid propagation method for in vitro of docynia delavayi |
CN114652806A (en) * | 2022-05-13 | 2022-06-24 | 云南中医药大学 | Dai medicine composition for treating chloasma as well as preparation method and application thereof |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106038738A (en) * | 2016-04-18 | 2016-10-26 | 四川师范大学 | Docynia delavayi polyphenol extract and preparation method thereof |
CN106038738B (en) * | 2016-04-18 | 2020-06-12 | 四川师范大学 | Docynia plant polyphenol extract and preparation method thereof |
CN111758576A (en) * | 2020-08-04 | 2020-10-13 | 西南林业大学 | Rapid propagation method for in vitro of docynia delavayi |
CN111758576B (en) * | 2020-08-04 | 2022-02-25 | 西南林业大学 | Rapid propagation method for in vitro of docynia delavayi |
CN114652806A (en) * | 2022-05-13 | 2022-06-24 | 云南中医药大学 | Dai medicine composition for treating chloasma as well as preparation method and application thereof |
CN114652806B (en) * | 2022-05-13 | 2023-07-07 | 云南中医药大学 | Dai medicine composition for preventing, improving or treating chloasma and preparation method and application thereof |
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